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CID : 2416900741
Name : MR.PRAVIN BHASATE
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Age / Gender : 34 Years / Male Application To Scan the Code

Consulting Dr. :- Collected : 17-Jun-2024 / 09:28

Reg. Location : Swargate, Pune (Main Centre) Reported : 17-Jun-2024 / 13:32

HIV 1+O/2 Antibodies & HIV 1 p24 Antigen

PARAMETER RESULTS METHOD
HIV 1+O/2 Antibodies and HIV 1 Non Reactive ECLIA
p24 Antigen, Serum
Test specifications:
‡ CLIA: Relative Sensitivity: 100% (100/100) Relative Specificity: 100% (100/100) with a 95% confidence interval (CI) of ²¬
‡ ECLIA: Sensitivity 100%, Specificity 99.63%
‡ CMIA: Analytical sensitivity of < 50 pg/mL to HIV-1 p24 Ag, Specificity >/= 99.5% interval (CI) of ²
‡ ELFA: Sensitivity -100.00% (95% confidence interval: 99.29% - 100.00%).
‡ Tridot (Immunofiltration)- Sensitivity:100%, Specificity:100%
Intended Use:
‡ The HIV Ag/Ab (Generation IV) assay is for the simultaneous qualitative detection of HIV p24 antigen and antibodies to human
immunodeficiency virus type 1 and/or type 2 (HIV-1/HIV-2) in human serum or plasma.
‡ This assay is intended to be used as an aid in the diagnosis of HIV-1/HIV-2 infection and as a screening test for donated blood and
plasma.
‡ An HIV Ag/Ab result does not distinguish between the detection of HIV p24 antigen, HIV-1 antibody, or HIV-2 antibody.
Clinical Significance:
‡ Human Immunodeficiency Virus (HIV) infection is the cause of Acquired Immunodeficiency Syndrome (AIDS) as well as symptomatic
disease prior to development of AIDS.
‡ HIV transmission is due to direct contact with infected body fluids; primarily blood, semen, vaginal and cervical secretions, breast milk
and amniotic fluid.
‡ The contact is usually mediated by sexual contact, IV drug abuse & blood exposure.
‡ Antibodies against HIV are nearly always detected in AIDS patients and HIV infected asymptomatic individuals.
‡ HIV 2 virus is similar to HIV 1 virus, however is less pathogenic, have longer latency period with slower progression to disease, lower
viral titres and lower rates of vertical and horizontal transmission.
Confirmatory Test: HIV RNA PCR
Limitations of the test:
‡ Heterophile antibodies in human serum can react with reagent immunoglobulins, interfering with in-vitro immunoassays.
‡ Patients routinely exposed to animals or animal serum products can be prone to this interference.
Reference:
‡ HIV kit pack insert
‡ Wallach's Interpretation of Diagnostic Tests
‡ Bakerman's ABC's of Interpretive Laboratory Data
Disclaimer: Pre and post counselling for HIV test will be performed by referring physician/authority whenever patient is referred.

*Sample processed at SUBURBAN DIAGNOSTICS (INDIA) PVT. LTD Pune Lab, Pune Swargate
*** End Of Report ***

Dr.KARAN MAURYA
D.N.B (Path)
Pathologist

Page 1 of 3
 Authenticity Check

CID : 2416900741
Name : MR.PRAVIN BHASATE
Use a QR Code Scanner
Age / Gender : 34 Years / Male Application To Scan the Code

Consulting Dr. :- Collected : 17-Jun-2024 / 12:23

Reg. Location : Swargate, Pune (Main Centre) Reported : 17-Jun-2024 / 14:35

EXAMINATION OF SEMINAL FLUID

PARAMETER RESULT BIOLOGICAL REF RANGE METHOD
Abstinence 2 days 2-7 days -
Collection time 12.18 pm -
Sample receival time 12.25 pm -
Complete collection Yes - -
PHYSICAL EXAMINATION
Ejaculate volume 2.0 ml >/=1.4 ml -
Color Creamish white Grey opalescent -
Odour Normal Normal -
Liquefaction More than 60 min Within 60 min (Normal) -
Viscosity Normal = <2 cm after liquefaction -
(Normal)
CHEMICAL EXAMINATION
pH 8.0 >/=7.2 pH Indicator
Fructose (Qualitative) Positive Positive Seliwanoff
MICROSCOPIC EXAMINATION
SPERM COUNT
No. of sperms / ml 8.5 million/ml >/=15 million/ml Microscopy
No. of sperms / ejaculate 17 million >/=39 million Calculated
MOTILITY
Rapidly Progressive (a) % 10 - Microscopy
Slow Progressive (b) % 35 - Microscopy
Non Progressive (c) % 10 - Microscopy
Immotile (d) % 45 <20 Microscopy
Total motility (a+b+c) 55 >/=42% Calculated
All progressive (a+b) 45 >/=30% Calculated
SPERM MORPHOLOGY
Total normal forms % 8 >/= 04 Microscopy
Abnormal head forms % 72 - Microscopy
Abnormal midpiece % 10 - Microscopy

Page 2 of 3
 Authenticity Check

CID : 2416900741
Name : MR.PRAVIN BHASATE
Use a QR Code Scanner
Age / Gender : 34 Years / Male Application To Scan the Code

Consulting Dr. :- Collected : 17-Jun-2024 / 12:23

Reg. Location : Swargate, Pune (Main Centre) Reported : 17-Jun-2024 / 14:35

Abnormal tail % 8 - Microscopy

Excessive residual cytoplasm % 2 - Microscopy

OTHER PARAMETER
Sperm aggregates Absent Absent Microscopy
Agglutination (Grade) Absent Absent Microscopy
Epithelial Cells / hpf 0-1 Microscopy
Round cells / hpf 0-1 0-2/hpf Microscopy
Red Blood Cells / hpf Absent 0-2 /hpf Microscopy

Note:

‡ Semen analysis (SA) aids in investigating the male fertility status as well as monitoring spermatogenesis during and following male
fertility regulation and other interventions.
‡ Certain key patient factors like age, testicular size, endocrine status, medications (like alpha blockers,SSRI), supplements or non-
prescribed medications (like anabolic steroids) may influence the outcome.
‡ The measurement of ejaculate characteristics depends on the activity of accessory glands, abstinence period and the completeness of
the ejaculate submitted.
‡ A high number of immotile and non-viable sperms may be due to epididymal pathology. A high number of immotile and viable sperms
may be due to structural defects in the flagellum.
‡ If the semen analysis is abnormal, then the analysis can be repeated 3 months after completion of another complete spermatogenesis
cycle. It can be repeated earlier if the sperm count is low or absent

Reference:

‡ WHO laboratory manual for the examination and processing of human semen, sixth edition. Geneva: World Health Organization; 2021.
‡ Sunder M, Leslie SW. Semen Analysis. [Updated 2022 Oct 24].
‡ Boitrelle, F.; Shah, R.Saleh, R.; Henkel, R.; Kandil, H.Chung, E.; Vogiatzi, P.; Zini, A Arafa, M.; Agarwal, A. The Sixth Edition of the
WHO Manual for Human Semen Analysis: A Critical Review and SWOT Analysis. Life 2021, 11, 1368

*Sample processed at SUBURBAN DIAGNOSTICS (INDIA) PVT. LTD Pune Lab, Pune Swargate
*** End Of Report ***

Dr.CHANDRAKANT PAWAR
M.D.(PATH)
Pathologist

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