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Class 12th Biology - MCQ Test Paper

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114 views4 pages

Class 12th Biology - MCQ Test Paper

Uploaded by

abubakrsiddique2704
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Class 12th Biology - MCQ Test Paper

Chapter: Biotechnology and Its Principles


Time: 1 Hour
Max Marks: 20

1. Which of the following is not a tool of recombinant DNA technology?


(a) Restriction enzymes
(b) DNA ligase
(c) Gel electrophoresis
(d) RNA polymerase

2. Plasmids are often used in genetic engineering because they:


(a) Can replicate independently
(b) Can transfer genes into plant cells
(c) Are found in all organisms
(d) Are resistant to antibiotics

3. EcoRI is an example of a:
(a) DNA polymerase
(b) Exonuclease
(c) Restriction endonuclease
(d) RNA polymerase

4. Which enzyme is used to join the DNA fragments?


(a) DNA helicase
(b) DNA ligase
(c) RNA polymerase
(d) Restriction endonuclease

5. The selectable marker gene in a vector helps in:


(a) Identifying the transformed cells
(b) Replication of the vector
(c) Restricting the growth of bacteria
(d) Cloning of the foreign gene

6. The origin of replication (ori) in a cloning vector is responsible for:


(a) Restricting DNA
(b) Controlling plasmid copy number
(c) Initiating transcription
(d) All of the above

7. The enzyme that cuts DNA at specific sequences, producing sticky ends or
blunt ends, is:
(a) DNA ligase
(b) Reverse transcriptase
(c) Restriction endonuclease
(d) DNA polymerase

8. The plasmid used by Agrobacterium tumefaciens to genetically modify plants


is called:
(a) F-plasmid
(b) T-plasmid
(c) R-plasmid
(d) Ti-plasmid

9. What is the purpose of Polymerase Chain Reaction (PCR) in biotechnology?


(a) To cut DNA at specific sites
(b) To amplify DNA
(c) To join DNA fragments
(d) To separate DNA fragments by size

10. DNA fragments are separated in gel electrophoresis based on their:


(a) Size
(b) Charge
(c) Shape
(d) Sequence

11. Which of the following steps is NOT a part of recombinant DNA technology?
(a) Isolation of DNA
(b) Cutting of DNA using restriction enzymes
(c) Amplification using RNA polymerase
(d) Ligation of DNA fragment into a vector

12. Which of the following organisms is most commonly used for the production
of recombinant insulin?
(a) Yeast
(b) Escherichia coli
(c) Bacillus subtilis
(d) Streptococcus

13. Restriction enzymes are used in genetic engineering because they:


(a) Bind to DNA and initiate replication
(b) Synthesize new DNA strands
(c) Cut DNA at specific sequences
(d) Amplify the DNA sequence

14. In recombinant DNA technology, the term competent cells refers to:
(a) Cells that can synthesize proteins efficiently
(b) Cells that have the ability to take up foreign DNA
(c) Cells that contain recombinant plasmids
(d) Cells that are used for cloning genes

15. The enzyme reverse transcriptase is used to:


(a) Replicate DNA
(b) Convert RNA into DNA
(c) Synthesize mRNA from DNA
(d) Cut DNA at specific sites

16. Which of the following is a function of DNA ligase in recombinant DNA


technology?
(a) Joining DNA fragments
(b) Replicating DNA
(c) Cutting DNA
(d) Amplifying DNA

17. The polymerase chain reaction (PCR) involves repeated cycles of:
(a) Denaturation, annealing, and extension
(b) Translation, transcription, and replication
(c) Separation, ligation, and transformation
(d) Transcription, translation, and transformation

18. pBR322 is a widely used plasmid vector. It contains genes for resistance to:
(a) Ampicillin and kanamycin
(b) Tetracycline and kanamycin
(c) Ampicillin and tetracycline
(d) Streptomycin and tetracycline

19. The insertion of a gene into a plasmid using a restriction enzyme results in:
(a) A recombinant plasmid
(b) A vector-free gene
(c) An antibody
(d) A ribozyme

20. Which of the following is used to introduce recombinant DNA into host cells?
(a) Micropipette
(b) Gel electrophoresis
(c) Biolistics or gene gun
(d) Restriction enzyme

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