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The report details an experiment measuring glucose concentration over time using a spectrophotometer to assess absorbance at 540 nm. Nine samples were taken at five-minute intervals for 40 minutes, with absorbance values recorded and averaged. The data was analyzed using the Beer-Lambert law to create a calibration curve for glucose concentration over time.

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16 views2 pages

Report TP

The report details an experiment measuring glucose concentration over time using a spectrophotometer to assess absorbance at 540 nm. Nine samples were taken at five-minute intervals for 40 minutes, with absorbance values recorded and averaged. The data was analyzed using the Beer-Lambert law to create a calibration curve for glucose concentration over time.

Uploaded by

serghaneomar81
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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REPORT TP : DIFFUSION

MADE BY :
-OMAR SERGHANE -SALMA NAAMA -HAMZA KOUJILI
-KAWTAR TOUZANI -RANIA SERROUKH

CONCENTRATION AT DIFFERENT TIME POINTS


By using the spectophotometer, we were able the measure the absorbance at 540 nm of
different samples, each taken at different time (every 5 minutes for 40 minutes) which
accounts to 9 samples including that taken at the beginning of the experiment. The
measuremants made by the machine can be witnessed in the following chart.

Absorbance Absorbance Average corrected


(experiment1) (experiment2) Absorbance

0.128 0.124 0.012

0.663 0.633 0.534

0.792 0.798 0.681

0.888 0.86 0.76

0.927 0.926 0.8125

0.991 1.033 0.898

1.032 1.014 0.909

By applying the equation of Beer-Lambert, we get to plot absorbance versus


glucose concentration, and get the following diagram :

1
Now we use the following calbration curve to obtain the concentration

Of glucose over time

Glucose concentration

0 250 292 313 333


354 375

Now we get to plot concentration versus time :

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