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Urine Test

The document outlines an experiment to test urine for glucose presence using Benedict's and Fehling's tests. It describes the materials needed, the theory behind the tests, the procedure for conducting the tests, and how to interpret the results based on the color of the precipitate formed. Precautions for conducting the experiment are also provided to ensure accurate results.

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rtamannasweety19
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8 views2 pages

Urine Test

The document outlines an experiment to test urine for glucose presence using Benedict's and Fehling's tests. It describes the materials needed, the theory behind the tests, the procedure for conducting the tests, and how to interpret the results based on the color of the precipitate formed. Precautions for conducting the experiment are also provided to ensure accurate results.

Uploaded by

rtamannasweety19
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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EXPERIMENT 23.2 Aim. To test the given sample of urine for the presence of sugar in it. Materials required Urine sample, test tubes, test tube stand, Benedict's solution, spirit lamp, Fehling's solution A, Fehling’s solution B etc. Theory In normal urine, practically there is no glucose. Presence of glucose in urine is called glucosuria, To detect reducing sugars such as glucose, fructose etc. in urine, Benedict's or Fehling’ tests are done, CuSO, present in Benedict's solution or Fehling’s solution is reduced on boiling by the reducing substances (glucose, fructose etc.) to form the coloured precipitate of cuprous oxide. The light green, green yellow and brick red precipitates of cuprous oxides depend on the concentration of reducing substances present in urine. Glucose reduces the blue cupric sulphate of Benedict's reagent of Fehling’s reagent to a coloured insoluble precipitate. CH,O(CHOH), CHO + 2Cu"S0,~ + 2H,0 Fay Glucose Cupric sulphate (reducing sugar) (@lue solution) CH,OH(CHOH), COOH + Cu,0 + 4H" + SO,~ Gluconie acid Cuprous oxide (oxidized sugar) (red precipitate) Procedure 1. Benedicts’s test Take 2 mL of urine sample in a test tube and add 2-3 mL of Benedict's solution to it. Boil it for about 2 minutes and then cool it to room temperature. Repeat the reaction with water and use it as control. 2. Febling’s test Take 2 mL of urine sample in a test tube and add 2 mL of Fehling’s A and 2 mL of Fehling’s B solution to it. Boil for about 2 minutes and then cool it to room temperature. Repeat the reaction with water and use it as control. Observations A reddish-yellow precipitate is formed showing the presence of glucose (reducing sugar) in it. Note: The colour of the precipitate formed shows the amount of glucose present in the sample of urine. If the colour of the precipitate formed is (@ yellowish-green, it shows that the urine sample has glucose in traces. (i) reddish-yellow, it shows that the urine sample has moderate amount of glucose. (iif) reddish-orange, it shows that the urine sample has large amounts of glucose. Precautions Same as in Experiment 23.1. DA Precautions 1. All the test tubes must be thoroughly washed before use. 2. All the heating must be done carefully and with the help of test tube holders, 3. Outdated reagents and chemicals must not be used, 4. |. Wash all the test tubes after the experiment,

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