Register your instrument!

www.eppendorf.com/myeppendorf

CellXpert® C170i

Operating Manual
Copyright © 2022 Eppendorf SE, Germany. All rights reserved, including graphics and images. No part of
this publication may be reproduced without the prior permission of the copyright owner.

Eppendorf® and the Eppendorf Brand Design are registered trademarks of Eppendorf SE, Germany.

CellXpert® and VisioNize® are registered trademarks of Eppendorf SE, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

The software included in this product contains copyrighted software that is licensed under the GPL. A copy
of that license is included in the settings of the device. You may obtain the complete corresponding source
code from us for a period of three years after our last shipment of this product. Please direct your request to
eppendorf@eppendorf.com.

Operating
CellXpert®
on
see
p.
Tab.
Fig.
p.
English
Operating Manual
C170i
(EN)
Manual

6731 900.010-09/052022
 Table of contents
CellXpert® C170i 3
English (EN)

Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.4 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3 Personal protective equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.4 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4.1 Description of ATEX Guideline 94/9EC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.5.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.6 Warning signs on the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1 Product overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1.1 CellXpert C170i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1.2 Inside the chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
3.1.3 Inner doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3.1.4 Water level sensor (option) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.1.5 Relative humidity sensor (option) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.1.6 Connectivity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4.1 Utilities requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4.3 Positioning the incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.4 Initial setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.4.1 Leveling the incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.4.2 Fixing the incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
4.5 Making connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
4.5.1 Electrical connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
4.5.2 Gas connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
4.5.3 O2 sensor (option) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
4.6 Door damper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
4.6.1 Installation of the door damper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
4.6.2 Disassembly of the door damper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
4.7 Alarm relay for Building Management System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
4.7.1 Incubator operating with and without alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
4.8 Connecting to VisioNize Lab Suite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
4.8.1 Registration to VisioNize Lab Suite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
 Table of contents
4 CellXpert® C170i
English (EN)

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.1 Preparing for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.2 Functions and limitations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.1 Temperature management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.2 Gas pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.3 CO2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.4 O2 (option). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.5 Relative humidity (option). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.2.6 Water level (option). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.2.7 Feature for maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.3 Opening and closing doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
5.3.1 Opening the inner and the outer door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
5.3.2 Closing the inner and the outer door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
5.3.3 Opening and closing door segments (option). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
5.4 Using the water tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
5.4.1 Using the water tray with the optional water level sensor . . . . . . . . . . . . . . . . . . . . . . 53
5.5 Using the access ports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
5.6 Safety shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
5.6.1 Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
5.6.2 Gas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
5.7 Switching off the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.1 Intuitive operating concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.2 Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.3 Home screen overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3.1 Status bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3.2 Function area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.3.3 Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.4 Information bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.4.1 Editing the information bar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.4.2 Editing the message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7 Starting the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

7.1 Operating the user interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.2 Selecting functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.3 Setting the values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
7.3.1 Setting a value with the slider. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
7.3.2 Changing a value incrementally with the fine adjustment . . . . . . . . . . . . . . . . . . . . . . 66
7.3.3 Selecting the last used value. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.3.4 Setting a value with the number pad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.3.5 Activate or deactivate the gas control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.4 Switching between the functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

8 Menu area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.1 Selecting the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
8.2 Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
8.2.1 The About this CellXpert C170i menu item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
8.2.2 The System Settings menu item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
8.2.3 The Device Settings menu item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
 Table of contents
CellXpert® C170i 5
English (EN)

8.3 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
8.3.1 Calling up the alarm overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
8.3.2 Setting alarms and alarm limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.4 Events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
8.4.1 Retrieving events. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
8.4.2 Filtering events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
8.4.3 Calling up more information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
8.4.4 Exporting events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
8.5 Charts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
8.5.1 Opening the chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
8.5.2 Selecting functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
8.5.3 Selecting the time span. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
8.5.4 Displaying values from the chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
8.5.5 Exporting the chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
8.6 Export . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
8.7 Recurring tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
8.7.1 Predefined tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
8.7.2 Perform a recurring task . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
8.7.3 Edit a recurring task . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
8.7.4 Define a new recurring task . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
8.8 Operation Records . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.8.1 Accessing Operation Records . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.8.2 Creating an experiment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.8.3 Filtering entries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.8.4 Exporting entries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.8.5 Displaying information on an experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.9 Screen Lock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.9.1 Locking the touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.9.2 Unlocking the touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
8.9.3 Activating or deactivating the Automatic Screen Lock . . . . . . . . . . . . . . . . . . . . . . . . 101

9 User administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

9.1 The user administration concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.1.1 User roles for working with user administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.1.2 Working without user administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.1.3 User rights. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.2 Establishing the user management. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.2.1 Creating an administrator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.2.2 Editing the user administration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
9.2.3 Deactivating the user administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.3 User login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
9.4 Editing user accounts as an administrator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
9.4.1 Creating a user account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
9.4.2 Editing user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
9.4.3 Deleting a user account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
9.4.4 Resetting the password/PIN for a user account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
9.5 Administrating your own user account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
 Table of contents
6 CellXpert® C170i
English (EN)

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.1.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.1.2 Daily checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.1.3 Weekly checks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.1.4 Monthly checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.1.5 Yearly checks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
10.2 Performance check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
10.3 Exterior cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
10.3.1 Cleaning the touch screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
10.4 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
10.4.1 Preparing the disinfection/decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
10.4.2 Disinfecting the exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
10.4.3 Disassembling the interior equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
10.4.4 Disinfecting/Decontaminating the interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
10.4.5 Reassembling the interior equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
10.4.6 Putting into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
10.5 High-temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
10.6 Contamination Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
10.6.1 Set the status to Contaminated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
10.6.2 Reset the status to Not Contaminated. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134

11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
11.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135

12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147

12.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
12.1.1 Conditions of transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
12.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
12.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

13 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149

13.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
13.1.1 Equipment dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
13.1.2 Internal dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
13.1.3 Floor space required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
13.1.4 Transporting dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
13.1.5 Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
13.1.6 Stacked CellXpert C170i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
13.2 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
13.3 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
13.4 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
13.4.1 Temperature management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
13.4.2 High-temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
13.4.3 CO2 control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
13.4.4 Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
13.4.5 O2 control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
13.4.6 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
 Table of contents
CellXpert® C170i 7
English (EN)

14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155

14.1 Shelves and shelf rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
14.2 Gas system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
14.3 Electrics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
14.4 Connectivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
14.5 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
14.6 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
14.7 Upgrade options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
 Table of contents
8 CellXpert® C170i
English (EN)
 Operating instructions
CellXpert® C170i 9
English (EN)

1 Operating instructions
1.1 Using this manual

 Read this operating manual completely before using the device for the first time. Observe the
instructions for use of the accessories where applicable.
 This operating manual is part of the product. Please keep it in a place that is easily accessible.
 Enclose this operating manual when transferring the device to third parties.
 The current version of the operating manual for all available languages can be found on our webpage
www.eppendorf.com/manuals.

1.2 Danger symbols and danger levels

The safety instructions of this operating manual indicate the following danger symbols and danger levels:

1.2.1 Danger symbols

Hazard point Hot surface

Electric shock Material damage

Explosion Heavy load

Inhalation Danger of cuts

Biohazard Magnetic fields

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
 Operating instructions
10 CellXpert® C170i
English (EN)

1.3 Symbols used

Depiction Meaning
1. Actions in the specified order
2.
 Actions without a specified order
• List
Text Display or software texts
Additional information

1.4 Glossary

High-temperature disinfection/HTD: Germ reducing method used to destroy microorganisms with

temperatures of 120-180 °C.

Relative humidity/RH: The ratio of the amount of water vapor present in the air at a specific temperature to
the maximum amount that the air could hold at that temperature, expressed as a percentage.

Stacking stand: Rack system for at most two devices to place them on top of each other and to fix them to
the wall.

VisioNize touch enabled device: Device from Eppendorf, which comes with the VisioNize touch interface.
VisioNize touch enabled devices are able to connect with the web application VisioNize Lab Suite, when
connected to the Internet.

VisioNize Lab Suite: VisioNize Lab Suite is a cloud-based platform where customers can access services
that can be tailored to their specific needs. The VisioNize Lab Suite is designed as a provider-independent
solution and works with different degrees of connectivity from Eppendorf devices to devices from
third-party suppliers.

VisioNize Services: Applications for data management, notification by e-mail/SMS, remote monitoring,
maintenance planning, and for accessing device-related documents of connected and non-connected
devices. Use of VisioNize Lab Suite and Services requires purchase of additional subscription. For more
information: https://www.eppendorf.com/visionize
 Safety
CellXpert® C170i 11
English (EN)

2 Safety
2.1 Intended use

The CellXpert C170i CO2 incubator is designed to and serve to provide a stable and homogeneous
atmosphere required for cell culture by controlling temperature, carbon dioxide, and optionally oxygen for
cultivating samples and cells from biological laboratories. It is intended exclusively for use indoors and for
operation by qualified staff for research purposes only.

CAUTION! Lack of safety due to incorrect accessories or spare parts

 Accessories and spare parts that are not recommended by Eppendorf compromise the
safety, functions, and precision of the device. Eppendorf cannot be held liable or accept
any liability for damage resulting from the use of non-recommended accessories and spare
parts.
 Only use accessories and original spare parts recommended by Eppendorf.

2.2 User profile

The device and accessories may only be operated by trained and skilled personnel.

Before using the device, read the operating manual carefully and familiarize yourself with the device’s
mode of operation.

CAUTION! Risk of personal injury or damage to equipment due to misappliance of

equipment

 The equipment must be operated as described in this manual. If operational guidelines are
not followed, equipment damage and personal injury can occur.
 Do not use the equipment in a hazardous atmosphere or with hazardous materials for
which the equipment was not designed.
 Eppendorf is not responsible for any damage to the equipment that may result from the
use of an accessory not manufactured by Eppendorf.

2.3 Personal protective equipment

Personal protective equipment protects your life and your health.

 Always wear the personal protective equipment required for the biosafety level and by the laboratory
regulations.
 Always wear protective clothing, protective gloves, and safety boots.
 If additional protective equipment is required, this is indicated above the respective instruction.
 Safety
12 CellXpert® C170i
English (EN)

2.4 Application limits

2.4.1 Description of ATEX Guideline 94/9EC

DANGER! Explosion hazard

 Do not operate the device in areas where work is completed with explosive substances.
 Do not use this device to process any explosive or highly reactive substances.
 Do not use this device to process any substances which could create an explosive
atmosphere.

DANGER! Explosion hazard

 Use gases in this equipment only within the range between their lower explosion limit
(LEL) and their upper explosion limit (UEL).
 If your process requires or produces gases, be sure to verify their LEL and UEL
concentration range (available online or ask your gas supplier).

CAUTION! Lack of safety due to incorrect use of materials

 Do not use this device to process any flammable materials, or use materials where the
transfer of mechanical energy to glass apparatus could lead to breakage.

Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially
explosive atmospheres.

The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab.

The use of substances which may contribute to a potentially explosive atmosphere is not permitted.

The final decision regarding the risks associated with using these types of substances is the user's
responsibility.
 Safety
CellXpert® C170i 13
English (EN)

2.5 Warnings for intended use

Before using the device, read the operating manual and observe the following general safety instructions.

2.5.1 Personal injury and damage to device

DANGER! Unconsciousness and death by elevated levels of CO2

Elevated levels of CO2 may be found in the operating area of the CO2 incubator.

 Wear personal protective equipment.

 If the laboratory is not properly ventilated, use a CO2/O2 alarm system.
 Check the tube connection system with a leakage test.
 Read the Information on performing a risk assessment for the operation of incubators with
CO2 and N2 from Eppendorf SE.

WARNING! Lack of safety due to incorrect gas installation or insufficient ventilation

 Only trained personnel may install and connect the gas tubing.

WARNING! Risk of personal injury and of material damage

Heavy devices placed on the incubator may topple and injure persons or cause damage to the
material.

 Only stack one CellXpert incubator on top of the other CellXpert incubator. Use the
stacking stand and the safety fastening kit.
 Do not place any other heavy devices on the incubator.

WARNING! Risk of suffocation

 Observe the national guidelines on handling gases and on the equipment and operation of
laboratories.
 Prevent a too high concentration of CO2 in breathing air while working in the laboratory.
 Prevent a displacement of O2 in breathing air due to working with N2.

WARNING! Risk of personal injury

Burns due to hot surface.

 Do not touch the equipment during the high-temperature disinfection cycle.

 Do not open the doors during the cycle.
 Do not open the doors after a system crash or a mains/power outage during the
high-temperature disinfection.
 Safety
14 CellXpert® C170i
English (EN)

WARNING! Risk of personal injury

Scalding due to hot water.

 Remove water from the water tray before starting the high-temperature disinfection cycle.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national regulations,
the biosafety level of your laboratory, and the manufacturers' Safety Data Sheets and
application notes.
 Wear your personal protective equipment.
 Consult the "Laboratory Biosafety Manual" (source: World Health Organization, Laboratory
Biosafety Manual, as amended) for comprehensive regulations on the handling of germs or
biological material of risk group II or higher.

WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.

 Wear your personal protective equipment.

 Observe the national regulations for handling these substances.
 Observe the manufacturers' Safety Data Sheets and application notes.

WARNING! Electric shock due to damage to the device or mains/power cord.

 Only switch on the device if the device and the mains/power cord are undamaged.
 Only operate devices which have been installed or repaired properly.
 In case of danger, disconnect the device from the mains/power supply voltage. Disconnect
the mains/power plug from the device or the earth/grounded socket. Use the
disconnecting device intended for this purpose (e.g., the emergency switch in the
laboratory).

WARNING! Danger due to strong magnetic field

Magnetic fields may affect pacemakers and defibrillators. Pacemakers may be reset.

 Keep a distance of at least 20 cm from the magnet.

WARNING! Risk of personal injury

 Do not open the device!

 Do not operate a broken device! (E.g. if the exterior is damaged)
 Do not modify the device!

WARNING! Risk of personal injury and equipment damage

Single devices or stacked devices on the stacking stand may tip over if not secured with the
safety latch.

 Secure basic devices or two stacked devices on the stacking stand to the wall with the
safety latch.
 Safety
CellXpert® C170i 15
English (EN)

CAUTION! Risk of personal injury

 At least 4 people are required to safely lift the incubator.

 The incubator has a high center of gravity. Make sure that it does not topple over when it is
moved.

CAUTION! Risk of personal injury

 Observe the safety standards of your lab.

 Use your personal protective equipment.

CAUTION! Risk of personal injury

 Before closing the glass door, make sure that the shelves are properly installed inside the
chamber. Slamming the glass door against the shelf could cause broken glass and injury.

NOTICE! Material damage due to incorrect connections.

 Only make electrical connections to devices that are described in the operating manual.
 Other connections are only permitted the consent of Eppendorf SE.
 Only connect devices that meet the safety requirements defined in DIN EN 62368-1.

NOTICE! Damage to the incubator

Lifting the incubator by the door will cause permanent damage to the incubator.

 Lift the incubator only by the lifting handles.

 Never lift the incubator by its door.

NOTICE! Damage to door latch and hinge

 Do not lean on the door.

 Do not put any items on the door.

NOTICE! Risk of material damage

 Never put any liquid material on the top of the incubator. Spilled liquid could cause a short
circuit. This will cause permanent damage to the incubator.

NOTICE! Risk of material damage

 To avoid possible damage to the CO2 sensor due to condensation, never leave water in the
water tray while the incubator is switched off, or when a high-temperature disinfection
cycle is initiated.
 Safety
16 CellXpert® C170i
English (EN)

NOTICE! Risk of material damage

The gas tubing and in-line gas filter will burst or crack due to high pressure.

 Incoming CO2 and N2 gas pressure must not exceed 0.15 MPa (1.5 bar, 21.8 psi).

NOTICE! Risk of material damage

 The instrument or equipment, and its external connections, to be used inside the chamber
should be specified as suitable for use in a humid environment, and at 37 °C. If in doubt,
consult with the manufacturer of the equipment.
 Always ensure that the connections are properly and securely made.
 Instruments transfer heat into the chamber. Do not allow too much heat to be transferred
into the chamber. Too much heat will affect the temperature control or activate the
temperature limiter; this could cause the loss of sample.

NOTICE! Risk of material damage

 Do not modify the device; this could cause the loss of sample.

NOTICE! Damage due to overheating.

 Do not place the device close to sources of heat (e.g., radiator, drying cabinet).
 Do not expose the device to direct sunlight.
 Ensure that all sides of the device are an adequate distance from the wall and adjacent
devices to guarantee unobstructed air circulation.

NOTICE! Damage to device or malfunctions due to a damaged touch screen.

 Do not operate the device.

 Switch off the device, disconnect the mains/power plug and have the touch screen
replaced by a service technician who has been authorized by Eppendorf.

The mains/power switch and the disconnecting device for the mains/power line must be easily
accessible during operation (e.g., a residual current circuit breaker).

Do not use a USB stick with a cable.

If high-temperature settings are used, be aware of heated parts, when touching the incubator
or coming into contact with interior parts.
 Safety
CellXpert® C170i 17
English (EN)

2.6 Warning signs on the device

Symbol Meaning Location
Hazard point On the name plate at the right
side next to the mains/power
 Observe the operating manual switch

Risk of burns from hot surfaces Top left and right on the door
panel, visible when outer door is
opened

Read the operating manual Right side, next to the mains/

power switch

Warning Top left and top right on the front

Strong magnetic fields produced panel, visible when outer door is
by the magnets used for door opened.
locking Right side next to the door closing
magnets (left side when door
hinge is on the right side).
 Safety
18 CellXpert® C170i
English (EN)
 Product description
CellXpert® C170i 19
English (EN)

3 Product description
3.1 Product overview
3.1.1 CellXpert C170i
Abb. 3-1: CellXpert C170i: Front and rear view

1 2 3 4 5

C170

10 9 8 7 6

Fig. 3-1: CellXpert C170i: Front and rear view

1 Door hinge 6 N2 gas connection

(option)
2 Control panel
7 Hole for installing the lifting handle
3 Door handle
(4)
4 Access ports
8 Name plate
5 CO2 gas connection
9 Mains/power switch
10 Service interface
 Product description
20 CellXpert® C170i
English (EN)

Abb. 3-2: CellXpert C170i: Control unit

Fig. 3-2: CellXpert C170i: Control unit

1 Touch screen 2 USB port

 Product description
CellXpert® C170i 21
English (EN)

Abb. 3-3: CellXpert C170i: Connections at the rear of the device

1 2

CO2

8 Intranet

Expansion
7 Connector

6
BMS

CH.4

CH.3

N2
CH.2

CH.1

3
5
Power

4 3

Fig. 3-3: CellXpert C170i: Connections at the rear of the device

1 Upper access port 5 Mains/power cord socket

2 CO2 tube connector 6 Connection for Building Management System
(BMS)
3 N2 tube connector
4 channels
(option)
7 Expansion connector
4 Lower access port
(option)
8 Connection for intranet
 Product description
22 CellXpert® C170i
English (EN)

3.1.2 Inside the chamber

Abb. 3-4: CellXpert C170i: Inside the chamber

1 2

6
7

Fig. 3-4: CellXpert C170i: Inside the chamber

1 Humidity sensor 5 Upper access port

(option)
6 Lower access port
2 Combined inlet for N2 gas (option) and for CO2
7 Water level sensor
gas
(option)
3 CO2 sensor
4 O2 sensor
(option)
 Product description
CellXpert® C170i 23
English (EN)

3.1.3 Inner doors

There are three options available for the inner doors:

• Non-segmented inner door
• Inner door with 4 door segments
• Inner door with 8 door segments
Abb. 3-5: Non-segmented inner door

Fig. 3-5: Non-segmented inner door

1 Inner door latch 2 Sample port

 Product description
24 CellXpert® C170i
English (EN)

Abb. 3-6: Inner door with 4 door segments

1
2

Fig. 3-6: Inner door with 4 door segments

1 Inner door latch 3 Sample port

2 Door segment handle
 Product description
CellXpert® C170i 25
English (EN)

Abb. 3-7: Inner door with 8 door segments

1
2

Fig. 3-7: Inner door with 8 door segments

1 Inner door latch 3 Sample port

2 Door segment handle
 Product description
26 CellXpert® C170i
English (EN)

3.1.4 Water level sensor (option)

The water level sensor is located above the water tray. The flexible end of the water level sensor allows the
user to remove and insert the water tray easily.

3.1.5 Relative humidity sensor (option)

The humidity sensor is located near the top left corner of the chamber. During the high-temperature
disinfection, the humidity sensor must be covered with the protective cover.

3.1.6 Connectivity

CellXpert C170i can be integrated into the digital solution of Eppendorf: VisioNize Lab Suite.

VisioNize Lab Suite is a cloud-based platform intended to be used for:

• connecting internet-enabled laboratory devices for data management, notification services by e-mail/
SMS, remote monitoring, maintenance planning, and for accessing device-related documents
• manually managing laboratory devices for data management, maintenance planning, and for accessing
device-related documentation

Learn more about VisioNize Lab Suite by visiting www.eppendorf.com/visionize

Accessories are required for connecting to VisioNize (see Connectivity on p. 155).

 Product description
CellXpert® C170i 27
English (EN)

3.2 Delivery package

 Check whether the delivery is complete.

 Check all parts for transport damage.
 Keep the transport box and packing material in order to transport and store the device
safely.

Report any damage to the carrier and to your local Eppendorf sales order department immediately.

Quantity Description Notes

1 Device Provided
1 Operating manual Provided
1 Information on performing a risk assessment Provided
for the operation of incubators with CO2 and
N2
4 Non-tip perforated shelf Installed
1 Shelf rack Installed
1 Water tray Installed
2 Plug for access port Installed
1 (O2 option) O2 sensor Installed
1 (H2O level Water level sensor Installed
option)
1 (rH sensor Relative humidity sensor Installed
option)
1 (rH sensor Protective cover for relative humidity sensor Packed in accessory kit
option)
1 Mains/power cord Packed in box inside outer carton
1 10 mm (0.4 in) outer diameter tubing with Packed in accessory kit
(2 for O2 option) 6.5 mm (0.2 in) inner diameter with in-line
gas filter
Length: 3 m
4 Lifting handle Packed in accessory kit
3 Spare shelf rack foot Packed in accessory kit
3 Spare shelf rack spacer Packed in accessory kit
4 Silicone foot Packed in accessory kit
1 Safety fastening kit Packed in accessory kit
1 BMS plug Packed in accessory kit
2 Open-end wrench for adjusting the feet Packed in accessory kit
(8 mm and 13 mm)
4 Non-slipping cap for adjustable feet Packed in accessory kit
1 Door damper Packed in accessory kit
 Product description
28 CellXpert® C170i
English (EN)
 Installation
CellXpert® C170i 29
English (EN)

4 Installation
4.1 Utilities requirements

The following utilities requirements are needed for operation:

Utility Requirement
Electricity Use an earthed/grounded mains/power supply which corresponds to the
electrical prerequisites on the name plate. The mains/power supply has to be
equipped with a residual current circuit breaker.
Mechanical Use the safety fastening kit for fixing the incubator or 2 stacked incubators to
the wall.
CO2 gas Cylinder with 100 % CO2 vapor withdrawal, together with a two-stage
regulator for pressure control between 0.05 MPa and 0.15 MPa (7.2–21.8 psi,
0.5–1.5 bar)
For O2 option: N2 gas Cylinder with 100 % N2 vapor withdrawal, together with a two-stage regulator
for pressure control between 0.05 MPa and 0.15 MPa (7.2–21.8 psi, 0.5–
1.5 bar)

Keep the packaging and the transport securing device for later transport or storage.

4.2 Selecting the location

CAUTION! Lack of safety due to humid storage and transport conditions

 Evaluate the device for condensation, when received and unpacked for installation.
 If condensation due to storage and transport in humid conditions has occurred, allow the
device to dry out for 12 hours in an environment of 18–28 °C and 20–80 % relative
humidity. Only with a drying-out process is the device able to meet safety requirements.

NOTICE! Damage due to overheating.

 Do not place the device close to sources of heat (e.g., radiator, drying cabinet).
 Do not expose the device to direct sunlight.
 Ensure that all sides of the device are an adequate distance from the wall and adjacent
devices to guarantee unobstructed air circulation.

The mains/power switch and the disconnecting device for the mains/power line must be easily
accessible during operation (e.g., a residual current circuit breaker).

The main gas valve must be easily accessible during operation.

 Installation
30 CellXpert® C170i
English (EN)

Do not place the incubator directly on the floor as it will aspirate dust.

The CellXpert C170i fits on the workbench, under a workbench or it can be double-stacked to save space.
Select a level surface capable of withstanding the operating weight of the incubator. The actual operating
weight depends on both the options installed, and the material stored in the incubator.

Minimum floor space required

• Width: 81 cm (31.9 in)
• Depth: 60 cm (23.6 in)
• Height: 95 cm (37.4 in)

Optimal distance
• 15 cm (6 in) at the door hinge side (opening of 90°)
• 10 cm (4 in) at the opposite side for mounting the lifting handles

The correct location features

• a minimum ventilation clearance of 5 cm (2 in) to the left and to the right
a minimum ventilation clearance of 5 cm (2 in) upward
a minimum ventilation clearance of 3 cm (1.2 in) at the rear side
• a minimum clearance of 5 cm (2 in) on the right side to use the mains/power switch
• protection from direct sunlight
• protection from vibrations
• a clear distance to heat sources, e.g., heaters, autoclaves, or drier compartments
• a clear distance to airflow of heating, air-conditioning ducts, or other drafts
• a clear distance to cold sources, e.g., ultra-low temperature freezers
• an even, stable ground to avoid the device falling down

The electrical connections feature

• a matching of the mains/power connection with the specifications on the name plate
• access to the cutting unit of the power system circuit (to unplug the mains/power cord) during operation

Unplug the device to completely disconnect it from the power source. Install the device in a way that allows
easy access to the mains/power plug in case of emergency.
 Installation
CellXpert® C170i 31
English (EN)

4.3 Positioning the incubator

WARNING! Danger due to strong magnetic field

Magnetic fields may affect pacemakers and defibrillators. Pacemakers may be reset.

 Keep a distance of at least 20 cm from the magnet.

CAUTION! Risk of injury from lifting and carrying heavy loads

 At least 4 people are required to safely lift the incubator.

 Use a transport aid for transporting the incubator.

CAUTION! Risk of personal injury

 The incubator has a high center of gravity. Make sure that it does not topple over when it is
moved.

NOTICE! Damage to the incubator

Lifting the incubator by the door will cause permanent damage to the incubator.

 Lift the incubator only by the lifting handles.

 Never lift the incubator by its door.

NOTICE! Damage to the incubator

Pushing, pulling, or turning the incubator on its feet and over barriers will cause damage to
the incubator feet.

 Only lift the incubator by the lifting handles.

 At least 4 people are required to safely lift the incubator.
 Check if the lock nuts are tightened to the bottom plate of the incubator.
 Installation
32 CellXpert® C170i
English (EN)

1 Holes for lifting handles

1. Install the 4 lifting handles into the tapped holes.

2. Carefully move the incubator to the operational location using the lifting handles.
3. Check to ensure that the door cable is not pinched and may be moved.

For non-slip application use the supplied non-slipping caps:

1. Lift the incubator on one side with the lifting handles.

2. Place one non-slipping cap under each of the 2 incubator feet.
3. Repeat the procedure on the other side of the incubator.
4. Remove the lifting handles and store them for further use.
5. Install the cover caps into the tapped holes on both sides of the incubator.
 Installation
CellXpert® C170i 33
English (EN)

4.4 Initial setup

4.4.1 Leveling the incubator

Prerequisites
A spirit level with the ends facing to the left and to the right is placed on one shelf.

Do not place the spirit level on the housing.

NOTICE! Risk of material damage

Due to an unleveled device, it is possible that not all cells are covered with the medium. This
could cause the loss of sample.

 Level the device by adjusting its feet.

NOTICE! Damage to the incubator

The feet may bend if they are turned out to far.

 Turn out the feet less than of 2 cm.

 Tight the lock nut on each foot.

1 2 3

13 mm

13 mm 8 mm

1. Loosen the lock nut with the supplied wrench of 13 mm.

2. Adjust the leveling feet with the wrench of 8 mm until the incubator is level and stable.
3. Turn the spirit level so that the ends face forward and rearward.
4. Level the incubator by adjusting the height of the feet.
5. Place the spirit level on other shelves. Adjust the height of the feet if necessary.
6. Lock the leveling feet by tightening the lock nuts on each foot with the wrench of 13 mm.
 Installation
34 CellXpert® C170i
English (EN)

4.4.2 Fixing the incubator

WARNING! Risk of personal injury and equipment damage

Single devices or stacked devices on the stacking stand may tip over if not secured with the
safety latch.

 Secure basic devices or two stacked devices on the stacking stand to the wall with the
safety latch.

WARNING! Risk of personal injury and equipment damage

When moving a stack of two incubators, the upper incubator may topple, if it is still fixed to
the wall.

 Disengage the safety latch before moving the incubators.

1. Disconnect the upper and the lower part of the

magnetic safety latch by pushing them aside from
each other.

2. To attach the lower part of the safety latch to the

top side of the incubator, use the spare part screw
M4x8, Torx 20, and a washer.
 Installation
CellXpert® C170i 35
English (EN)

3. Attach the other part of the safety latch and a

washer to the wall. The screw must have a pullout
strength of at least 250 N (25 kg).

4. Click the two parts of the magnetic safety latch

together.

5. Fasten the belt. Do not fasten too tightly.

 Installation
36 CellXpert® C170i
English (EN)

4.5 Making connections

4.5.1 Electrical connection

WARNING! Danger due to incorrect voltage supply.

 Only connect the device to voltage sources which correspond with the electrical
requirements on the name plate.
 Only use earth/grounded sockets with a protective earth (PE) conductor.
 Only use the mains/power cord supplied.

WARNING! High voltage

 Before taking this device in operation, always make sure that this equipment is properly
earthed / grounded by means of the supplied mains/power cord.

Do not use a multiple socket extension cord.

 Make sure that the voltage requirements match the information on the name plate.
 Incubators with 100 V – 127 V: Connect only 1 device to 1 fuse.
 Incubators with 220 V – 240 V: Connect up to 2 devices to 1 fuse.
 Use the mains/power cord supplied to connect the incubator to the correct mains/power supply voltage.

4.5.2 Gas connection

DANGER! Unconsciousness and death by elevated levels of CO2

Elevated levels of CO2 may be found in the operating area of the CO2 incubator.

 Wear personal protective equipment.

 If the laboratory is not properly ventilated, use a CO2/O2 alarm system.
 Check the tube connection system with a leakage test.
 Read the Information on performing a risk assessment for the operation of incubators with
CO2 and N2 from Eppendorf SE.

WARNING! Lack of safety due to incorrect gas installation or insufficient ventilation

 Only trained personnel may install and connect the gas tubing.

WARNING! Risk of suffocation

 Observe the national guidelines on handling gases and on the equipment and operation of
laboratories.
 Prevent a too high concentration of CO2 in breathing air while working in the laboratory.
 Prevent a displacement of O2 in breathing air due to working with N2.
 Installation
CellXpert® C170i 37
English (EN)

NOTICE! Risk of material damage

The gas tubing and in-line gas filter will burst or crack due to high pressure.

 Incoming CO2 and N2 gas pressure must not exceed 0.15 MPa (1.5 bar, 21.8 psi).

The main gas valve must be easily accessible during operation.

Only use dry gas.

Do not bend the gas tubing.

Abb. 4-1: Connections for CO 2 and N2 at the rear of CellXpert C170i

Fig. 4-1: Connections for CO2 and N2 at the rear of CellXpert C170i

1 CO2 tube connector 2 N2 tube connector (option)

1. Attach the longer part of the supplied gas tubing to the pressure regulator outlet. Ensure that the gas
supply is connected to the INLET side of the in-line gas filter. Attach the shorter part of the supplied gas
tubing to the CO2 tube connector at the rear of the incubator. Push the gas tubing into the tube
connector until it stops. Check if the gas tubing is fixed by lightly pulling on it. It must not move. Secure
the connected end at the pressure regulator.
 Installation
38 CellXpert® C170i
English (EN)

2. For O2 option: Attach the longer part of the supplied gas tubing to the pressure regulator outlet. Ensure
that the gas supply is connected to the INLET side of the in-line gas filter. Attach the shorter part of the
supplied gas tubing to the N2 tube connector at the rear of the incubator. Push the gas tubing into the
tube connector until it stops. Check if the gas tubing is fixed by lightly pulling on it. It must not move.
Secure the connected end at the pressure regulator.
3. A large-size cylinder of CO2 with vapor withdrawal (and optional a large-size cylinder of N2 with vapor
withdrawal) is required to supply the incubator. The cylinder controls primary gas pressure. Fitting a
two-stage CO2 (and optional N2) pressure regulator is recommended.
4. Check the pressure settings of the CO2 and the N2 connections.

A default pressure setting for CO2 of 0.1 MPa (14.5 psi, 1 bar) or in the range of 0.05–
0.15 MPa (7.2–21.8 psi, 0.5–1.5 bar) is recommended.
O2 option: A default pressure setting for N2 of 0.1 MPa (14.5 psi, 1 bar) or in the range of
0.05–0.15 MPa (7.2–21.8 psi, 0.5–1.5 bar) is recommended.
5. To disconnect the gas tubing, switch off the gas pressure and press down the small ring of the tube
connector and pull out the gas tubing.

4.5.3 O2 sensor (option)

Never disconnect the O2 sensor.

If the incubator is provided with the O2 option, the O2 sensor already is mounted. The sensor is individually
calibrated for each incubator. Do not use O2 sensors from other devices. If an O2 sensor is defective,
contact the authorized service.

4.6 Door damper

The door damper allows a soft closing of the outer door. If required, you can install the door damper
additionally.

Delivery package
• Door damper
• Cranked wrench key Tx10
• Flat head screwdriver
• Plug
 Installation
CellXpert® C170i 39
English (EN)

4.6.1 Installation of the door damper

The installation point is at the bottom of the inner side of the outer door.

Prerequisites
The holder for the door damper is installed.

1. Insert the longer and thicker part of the door

damper into the holder.

2. Press the door damper into the holder until it

stops.

4.6.2 Disassembly of the door damper

1. Remove the door damper with the flat head

screwdriver.
 Installation
40 CellXpert® C170i
English (EN)

4.7 Alarm relay for Building Management System

Only connect devices that meet the safety requirements defined in IEC 60950-1.

The connection to the Building Management System (BMS) allows the centralized monitoring of the device.
Alarm sources are programmable with the user interface.

The system is activated by alarm conditions:

• over-temperature
• under-temperature
• errors and warnings
• CO2 high
• CO2 low
• power failure

The power failure alarm cannot be switched off. If mains/power supply voltage fails or the device is being
switched off, all relays switch to alarm: Common and normally open contacts are connected.

The system is connected via a 12-pin connector at the rear of the incubator. The matching plug is provided.

Connect the device to the BMS with an unshielded cable harness of single-conductor or multi-conductor
type with a cross section of AWG 28–16 or 0.08–1.5 mm². Mount the ferrite core to the cable as described
in the instructions for use for the ferrite core.

The maximum cable length must be shorter than 30 m.

Abb. 4-2: BMS connector

BMS Pin
12
CH.4 11
10
9
CH.3 8
7
6
CH.2 5
4
3
CH.1 2
1

Fig. 4-2: BMS connector

 Installation
CellXpert® C170i 41
English (EN)

Pin Designation
1 Channel 1 common
2 Channel 1 normally closed
3 Channel 1 normally open
4 Channel 2 common
5 Channel 2 normally closed
6 Channel 2 normally open
7 Channel 3 common
8 Channel 3 normally closed
9 Channel 3 normally open
10 Channel 4 common
11 Channel 4 normally closed
12 Channel 4 normally open

The relays must not be operated at more than 2 A and 30 V DC/AC.

Default settings are:

Channel 1 (relay 1) Temperature alarm

Channel 2 (relay 2) CO2 alarm
Channel 3 (relay 3) O2 alarm (OFF, if option is not available)
Channel 4 (relay 4) Water level alarm (OFF, if option is not available)

The alarm sources for the channels can be programmed at the user interface.
 Installation
42 CellXpert® C170i
English (EN)

4.7.1 Incubator operating with and without alarm

Abb. 4-3: Incubator operating with alarm

Incubator with alarm triggered or power off

Ch x – normally closed

Ch x – normally open

BMS socket
Relay coil deenergized Relay contacts are floating/
by incubator control galvanically isolated,
because of alarm or isolation voltage max 30 V
power off to protective earth

Ch x – common

Fig. 4-3: Incubator operating with alarm

Abb. 4-4: Incubator operating without alarm

Incubator operating, no alarm

Ch x – normally closed

Ch x – normally open
BMS socket

Relay coil Relay contacts are floating/

energized by galvanically isolated,
incubator control isolation voltage max 30 V
when powered up to protective earth

Ch x – common

Fig. 4-4: Incubator operating without alarm

 Installation
CellXpert® C170i 43
English (EN)

4.8 Connecting to VisioNize Lab Suite

The following steps describe how to connect a device with VisioNize touch interface to VisioNize Lab Suite.

Requirements:
• Network socket of the local network near the device
• Standard Ethernet cable
• Internet access with open ports to the following URL:
– URL: www.eppendorf.com
– Port: 443 TCP
– Protocol: MQTT via web sockets
To avoid unauthorized access, data loss, sample loss, and data abuse, protect your VisioNize
touch enabled device against unauthorized access from the Internet.

 Ask your IT system administrator for advice.

Connect to the network

Prerequisites
All devices are integrated into the local network and have the port open to the following URL
www.eppendorf.com.

The software version of the device is compatible with VisioNize Lab Suite. (Check by tapping Menu >
Settings > About this device: scroll down to Software version.)

1. Connect the ethernet socket at the rear site of the

device to an unlocked socket of the network with
a standard ethernet cable.
2. To check the network connection, open the
settings on the touch screen of the device.
3. Tap Menu > Settings > System Settings > Network.

4. Switch on DHCP as network protocol.

 Installation
44 CellXpert® C170i
English (EN)

DHCP
5. If you do not use DHCP, switch off Enable DHCP
and tap Manual Setup.
6. Enter the device settings.

7. To check the entries, tap Back.

When the device is successfully connected, the IP
address is displayed next to IP Addresses.
If the IP address is not displayed, note the MAC
address and contact your local IT administrator.

8. To check if your device is correctly configured to

connect to VisioNize, use the function Check
Cloud Connectivity Prerequisites in Menu >
Contacts & Support > Diagnostics.
 Installation
CellXpert® C170i 45
English (EN)

4.8.1 Registration to VisioNize Lab Suite

VisioNize touch enabled devices already have the certificate to connect to VisioNize Lab Suite.

Log in to your VisioNize Lab Suite account by visiting: https://

<your_tenant_name>.visionizelabsuite.eppendorf.com. To request a demo or learn more about VisioNize
Lab Suite, visit https://www.eppendorf.com/visionize. To subscribe to VisioNize Lab Suite, visit: https://
www.eppendorf.com/visionize-subscription.

After logging in to your VisioNize Lab Suite account:

1. Add the device to your device list.

2. Navigate to the Device management.
3. Click Add device.
4. Select a VisioNize touch enabled device.
5. Enter the serial number.
6. Navigate to Menu > Settings > About this device at
the device display to check the serial number.
7. Confirm by clicking Submit.
8. Navigate to the tab Requests in the device
management app.
9. Select the device you want to add.
10. Click Accept device once you have connected the
device to your local network.
11. If the button Accept device is grayed out, check if
your device is connected to the local network.
 Installation
46 CellXpert® C170i
English (EN)
 Operation
CellXpert® C170i 47
English (EN)

5 Operation
5.1 Preparing for operation

DANGER! Unconsciousness and death by elevated levels of CO2

Elevated levels of CO2 may be found in the operating area of the CO2 incubator.

 Wear personal protective equipment.

 If the laboratory is not properly ventilated, use a CO2/O2 alarm system.
 Check the tube connection system with a leakage test.
 Read the Information on performing a risk assessment for the operation of incubators with
CO2 and N2 from Eppendorf SE.

NOTICE! Damage to electronic components due to condensation

Condensate can form in the device after it has been moved from a cool environment to a
warmer environment.

 After installing the device, wait for at least 12 hours. Only then connect the device to the
mains/power supply.

NOTICE! Risk of material damage

The gas tubing and in-line gas filter will burst or crack due to high pressure.

 Incoming CO2 and N2 gas pressure must not exceed 0.15 MPa (1.5 bar, 21.8 psi).

After switching on the incubator, sensors have to initialize. It may take a few minutes until
values are displayed.

1. Using the mains/power cord provided, connect the incubator to an earthed/grounded mains/power
supply.
2. Turn on the CO2 gas supply with the pressure regulator. Set the gas pressure to 0.1 MPa (1 bar,
14.5 psi). For O2 option: Turn on the N2 gas supply with the pressure regulator. Set the gas pressure to
0.1 MPa (1 bar, 14.5 psi).
The CO2 gas pressure range is adjustable between 0.05 MPa (flow pressure; 0.5 bar; 7.2 psi)
and 0.15 MPa (pressure at rest; 1.5 bar; 21.8 psi). The N2 gas pressure range is adjustable
between 0.05 MPa (flow pressure; 0.5 bar; 7.2 psi) and 0.15 MPa (pressure at rest; 1.5 bar;
21.8 psi).
Open the stop valve completely to ensure an adequate volume flow.
Ensure that the gas pressure and the volumetric gas flow rate are sufficient, especially if more
than one device is connected to the CO2 gas source.
 Operation
48 CellXpert® C170i
English (EN)

3. The chamber setpoints are pre-programmed at 37 °C and 5 % CO2.

• The CO2 sensor needs 30 minutes for initialization after the switch-on of the device. No
process value is displayed during the initialization phase.
• The O2 sensor (option) needs 10 minutes for initialization after the switch-on of the device.
No process value is displayed during the initialization phase.
• If power is interrupted to the incubator long enough for the temperature to drop below the
setpoint, the CO2 valve is deactivated until the temperature setpoint is achieved again.
(The deactivation serves to avoid spurious CO2 readings while the incubator is below its
temperature setpoint).
4. Switch on the incubator using the on-off switch at the right side of the device. Leave the incubator on
until the set chamber temperature and CO2 concentration have been reached.

The display illuminates immediately.

5. To allow conditions to stabilize, leave the incubator running for at least 2 hours (preferably overnight).
CO2 sensors are calibrated at the factory to control accurately at 5 % CO2 and 37 °C.
Changing the temperature or the CO2 setpoint leads to deviations in accuracy.
 Operation
CellXpert® C170i 49
English (EN)

5.2 Functions and limitations

5.2.1 Temperature management

The temperature is adjustable from 22 °C to 50 °C. Incubators are meant to operate with 4 °C above
ambient temperature at minimum. If the setpoint is below 4 °C above ambient temperature, the incubator
tries to adjust the temperature. It is possible that a sensor error occurs after a certain period. Restart the
incubator and adjust another setpoint.

Avoid condensation and reduce the temperature setpoint by:

• cooling down the incubator as fast as possible by opening the doors
• emptying and refilling the water tray with water with a maximum temperature of the new setpoint

5.2.2 Gas pressure

CO2 and N2 are connected to the tube connector. Gas pressures range from 0.05 – 0.15 MPa (7.2 – 21.8 psi,
0.5 – 1,5 bar). If the pressure is out of the range, the following reactions occur at certain values:

Pressure Value Reaction

> 1.8 bar The valve closes to protect the device. The CO2
control is stopped until the pressure is low enough.
< 0.2 bar An error occurs. The CO2 control is stopped until
the pressure is high enough.

You can switch off the pressure monitor for low gas pressure (see Gas system – Pressure Monitor on p. 81).

5.2.3 CO2

The CO2 concentration is adjustable from 0.1 % to 20 %. It is possible to deactivate the gas control and to
operate with temperature control only.

If you want to reduce the setpoint for the CO2 concentration during the operation, let CO2 escape by
opening the outer and the inner door.

The sensors have to reinitialize after a switch-on of the device or after a high-temperature disinfection took
place. The status Initializing Sensor is described in the function areas. It is not possible to adjust a setpoint
during this period.

5.2.4 O2 (option)

If you want to know whether the O2 option is installed on your device, tap Menu > Settings > About this
CellXpert C170i > Hardware Configuration.

The O2 concentration is adjustable from 1 % respective from 0.1 % to 20 % via N2 connection. It is

possible to deactivate the gas control.
 Operation
50 CellXpert® C170i
English (EN)

If the O2 sensor is temporarily not needed, deactivate the O2 sensor. Tap the Settings > Device Settings >
Home Screen Configuration menu items.

If you want to increase the setpoint for the O2 concentration during the operation, let O2 stream into the
incubator by opening the outer and the inner door.

It is not possible to adjust a high CO2 concentration together with a high O2 concentration. If setpoints have
been chosen beyond physical opportunities, an alarm message may occur.

After the switch-on of the device or after a high-temperature disinfection, the O2 sensor has to reinitialize.
The status Initializing Sensor is described in the function areas. It is not possible to adjust a setpoint during
the initialization.

A low O2 concentration of 1 % respective from 0.1 % to 20 % might lead to a formation of small droplets
outside the chamber. If small droplets appear, adjust the ambient conditions by increasing the ambient
temperature to >22 °C and/or by decreasing the relative humidity to <40 %.

5.2.5 Relative humidity (option)

The humidity sensor detects the relative humidity in the incubator. During a high-temperature disinfection,
it is necessary to protect the sensor with the cover. The alarm has a time-out for 3.5 h after the door closing
and for 5 h after the switch-on of the device.

5.2.6 Water level (option)

A hint appears on the screen, when the water level falls below 0.5 L. The hint recurs every 15 minutes until
the water tray is refilled. The alarm function is ready for action 2 hours after the door has been closed and
1 hour after the device has been switched on.

5.2.7 Feature for maintenance

The CellXpert C170i features the option of activating an automatic reminder for common tasks (see
Recurring tasks on p. 93).
 Operation
CellXpert® C170i 51
English (EN)

5.3 Opening and closing doors

WARNING! Danger due to strong magnetic field

Magnetic fields may affect pacemakers and defibrillators. Pacemakers may be reset.

 Keep a distance of at least 20 cm from the magnet.

CAUTION! Cuts due to broken glass

A damaged glass door leads to cuts on the hands.

 Remove the broken glass with a suitable tool.

CAUTION! Risk of personal injury

 Before closing the glass door, be sure that the shelves are properly installed inside the
chamber. Slamming the glass door against the shelf could cause broken glass and potential
injury.

5.3.1 Opening the inner and the outer door

1. To open the outer door, pull the door handle.

Abb. 5-1: Hint on the touch screen

Fig. 5-1: Hint on the touch screen

When the outer door is opened, a hint is displayed on the touch screen.
 Operation
52 CellXpert® C170i
English (EN)

2. Turn the inner door latch 90° to the top. To open the inner door, pull on the inner door latch.
Abb. 5-2: Inner door latch with magnet

1 2

Fig. 5-2: Inner door latch with magnet

1 Inner door latch closed 2 Inner door latch opened

5.3.2 Closing the inner and the outer door

1. Close the inner door and turn the inner door latch 90° to the side until it is fixed by the magnet.
The inner door is not correctly closed until the inner door latch is oriented horizontally. An
incorrectly closed door could cause condensation.
2. Close the outer door until it is fixed by the magnets.

5.3.3 Opening and closing door segments (option)

Prerequisites:

• A 4 or 8-segmented inner door is installed.

• The outer door is open.
1. To open the door segment, pull the door segment handle.

2. Close the door segment.

The handle is fixed by the magnet.
 Operation
CellXpert® C170i 53
English (EN)

5.4 Using the water tray

NOTICE! Risk of material damage

 To avoid possible damage to the CO2 sensor, never leave water in the water tray while the
incubator is switched off, or when a high-temperature disinfection cycle is initiated.

NOTICE! Risk of material damage due to spilled water

Spilled water could cause corrosion.

 To avoid possible damage to the device, observe the maximum capacity of the water tray.
 Do not move the device when the water tray is filled.

• Always leave the water tray in place.

• Use warm, distilled, sterile water only in the water tray. The usage of any other types of
water including deionized water may cause corrosion inside the incubator.

1. Fill the water tray with 1.5–2.5 liters of warm, distilled, sterile water.
2. The usage of any biocide in the water tray is not recommended. To reduce the possibility of
contamination, empty the tray every 7–14 days, clean it with a solution of 70 % isopropyl alcohol and
30 % distilled water, and then refill it with 1.5–2.5 liters of warm distilled, sterile water.
The humidity level within the chamber is not adjustable. The chamber reaches between 85 %
and 95 % relative humidity at 37 °C (depending on ambient humidity) with the water tray.

5.4.1 Using the water tray with the optional water level sensor

NOTICE! Risk of material damage

 Do not push parts of the interior equipment against the water level holder.

1. The flexible part of the water level sensor flips forward or backward when you pull out or push in the
water tray.
 Operation
54 CellXpert® C170i
English (EN)

5.5 Using the access ports

It is possible to insert components, e.g., sensors, into the chamber via the access ports.

Prerequisites

• The component, e.g., sensor, with wire is placed in the chamber.

• Both of the access ports are closed with the blind plugs.
• Make sure that the component is not bigger than the inner diameter of 25 mm (0.98 in) of the access
port.
1. Remove one of the blind plugs.
2. Pull the component wire through the open access port.
3. For optimal gas tightness, make a cut into the cover of the blind plug. Wrap the component wire around
the plug.
Abb. 5-3: Wire wrapped around the blind plug

Fig. 5-3: Wire wrapped around the blind plug

4. Refit the blind plug. Make sure that the plug is securely fixed and flush with the rear panel.
5. Put the end of the wire on the top of the incubator.

Make sure that the access port is cleaned and dried before reinstallation of the plug.
 Operation
CellXpert® C170i 55
English (EN)

5.6 Safety shutdown

NOTICE! Risk of material damage

The gas tubing and in-line gas filter will burst or crack due to high pressure.

 Incoming CO2 and N2 gas pressure must not exceed 0.15 MPa (1.5 bar, 21.8 psi).

5.6.1 Temperature

The CellXpert C170i features a shutdown at 10 K over the setpoint of one heat circuit or 5 K below the
setpoint according to standard DIN 12880 (temperature limiter class 1).

• Restart the device.

• If the error occurs again, contact Eppendorf Service.

5.6.2 Gas

The inlet gas valve switches off above 1.8 bar (0.18 MPa).

5.7 Switching off the device

1. Switch off the device.
2. Disconnect the power cord.
3. Switch off the gas pressure.
4. Disconnect the pressure tube.
5. Empty the water tray.
6. Clean the device.
7. Let the incubator dry with opened doors.
8. Let the incubator cool down.
 Operation
56 CellXpert® C170i
English (EN)
 Overview of operating controls
CellXpert® C170i 57
English (EN)

6 Overview of operating controls

6.1 Intuitive operating concept

Eppendorf offers a cross-product operating concept which supports swift familiarization with different
Eppendorf products. For different Eppendorf products, the basic operating controls are compatible with
each other by using an intuitive touch user interface.

6.2 Symbols
Symbol Description
Status: Function active

Status: Function inactive

Door open

Active error

Active alarm

Warning

Notice

Open alarm setting

Open event log

Open chart

Chart duration

Close

Filter

Export data

Change function position

Default alarm setting

 Overview of operating controls
58 CellXpert® C170i
English (EN)

Symbol Description
Fine increment adjustment

Display recently used values

6.3 Home screen overview

Abb. 6-1: Home screen

Fig. 6-1: Home screen

1 Status bar 3 Toolbar

Information on user logged in, date, time, device Buttons for navigation and operation of the
status, device name device
2 Function area
Standard functions of the device

6.3.1 Status bar

• Define the device name in Menu > Settings > About this CellXpert C170i.
• Log in as a user via User Management.
• Define time and date in Menu > Settings > System Settings > Date & Time.

If there is an active alarm, the exceeded alarm limit is highlighted in red.

 Overview of operating controls
CellXpert® C170i 59
English (EN)

6.3.2 Function area

Primary functions are displayed on the left. Additional functions and options are displayed on the right.

Abb. 6-2: Function area

1
6
2

4
7
5

Fig. 6-2: Function area

1 Name of the function 5 Setpoint of a non-controlled function

displayed in large black characters
2 Setpoint of a controlled function
displayed in small black characters 6 Chart
preview of the two most important functions
3 Actual value of a controlled function
displayed in large blue characters 7 Event log
preview of the notifications and messages
4 Actual value of a non-controlled function
displayed in small grey characters
 Overview of operating controls
60 CellXpert® C170i
English (EN)

6.3.3 Toolbar

1 2 3 4

1 Home 3 Disinfect
Tap the Home button to show the home screen. Tap the Disinfect button to start the
high-temperature disinfection program (see
2 Back
High-temperature disinfection on p. 127).
Tap the Back button to go the previous window.
4 Menu
Tap the Menu button to open functions such as
Settings, Alarms, or Events.
 Overview of operating controls
CellXpert® C170i 61
English (EN)

6.4 Information bar

If messages are present, the information bar appears instead of the status bar. The information bar displays
warning, alarm, and error messages.

Abb. 6-3: Information bar

1 2 3 4

Fig. 6-3: Information bar

1 Number of unacknowledged messages 3 Mute the audible alarm

Temporarily disables the audible alarm
2 Current messages
Red information bar: Unacknowledged alarm or 4 Acknowledge the current message
error messages When the current message has been
Yellow information bar: Unacknowledged acknowledged, it is deleted from the information
warning messages bar.
When all messages have been acknowledged, the
information bar disappears.
 Overview of operating controls
62 CellXpert® C170i
English (EN)

6.4.1 Editing the information bar

• To display the log of all unacknowledged messages, tap the number next to the bell symbol.
• To disable the audible alarm temporarily, tap the mute symbol.
• To confirm the current message, tap the checkmark symbol.

The alarm symbol is displayed until values are within the alarm limits.
 Overview of operating controls
CellXpert® C170i 63
English (EN)

6.4.2 Editing the message

• To call up the current message, tap the information bar. The message appears in the Event log.
• To acknowledge the message, tap the Acknowledge button.
• To change the alarm limits, tap the Alarm (bell symbol) symbol.
• To change the setpoint of the function, tap the button above the Alarm symbol.
 Overview of operating controls
64 CellXpert® C170i
English (EN)
 Starting the application
CellXpert® C170i 65
English (EN)

7 Starting the application

7.1 Operating the user interface

The touch screen is designed to be operated with your fingers only without the need for tools.
Laboratory gloves made of nitrile or latex can be worn. Alternatively, a stylus can be used, for
example, when thick gloves need to be worn in the laboratory.

If liquid comes into contact with the touch screen, the functions displayed on the touch screen
may be triggered.

 Do not drop any liquids onto the touch screen.

 Do not spill any liquids onto the touch screen.

7.2 Selecting functions

Tap the Temperature function in the function area. The parameter settings are displayed.
 Starting the application
66 CellXpert® C170i
English (EN)

7.3 Setting the values

The setpoints can be adjusted with the slider or with the number pad.

7.3.1 Setting a value with the slider

Tap the slider and drag it to the right or to the left.

The setpoint changes.

7.3.2 Changing a value incrementally with the fine adjustment

• Tap the + button. The setpoint is incrementally increased.

• Tap the - button. The setpoint is incrementally decreased.
 Starting the application
CellXpert® C170i 67
English (EN)

7.3.3 Selecting the last used value

1. Tap into the center of the fine adjustment. A list with the last used setpoints is displayed.
2. Select the setpoint from the list.
 Starting the application
68 CellXpert® C170i
English (EN)

7.3.4 Setting a value with the number pad

1. Tap the displayed setpoint. The number pad appears.

2. Enter the new setpoint.
3. Confirm your entry. The number pad disappears.
 Starting the application
CellXpert® C170i 69
English (EN)

7.3.5 Activate or deactivate the gas control

• To activate or to deactivate the CO2 control tap the status button.

• To activate or to deactivate the O2 control (option) tap the status button.
 Starting the application
70 CellXpert® C170i
English (EN)

7.4 Switching between the functions

To switch to another function, tap on the symbol of the required function. The active function is highlighted
in blue.
 Menu area
CellXpert® C170i 71
English (EN)

8 Menu area

The Menu area contains all of the software settings.

Abb. 8-1: The Menu screen

Fig. 8-1: The Menu screen

Settings Alarms
Information on the device and licenses Activate alarms and set alarm limits
Device, maintenance and system settings
Maintenance & Qualification
Activate user management
Perform recurring tasks
Contacts & Support
Login/Logout
Information on local contact persons
User management
Screen Lock
Users
Lock and unlock the touch screen
User profile settings
Clean the touch screen
Operation Records
Events
Define and export records of your experiments
Event log with messages and alarms
Charts
View functions in a diagram
Export
Export charts, logs, data and Operation Records
 Menu area
72 CellXpert® C170i
English (EN)

8.1 Selecting the menu

Tap the Menu button. The menu items available for the device are displayed.

Select a menu item.

 Menu area
CellXpert® C170i 73
English (EN)

8.2 Settings

This area provides you with information on the device. You can adjust device settings and enable the user
management.

• Tap the Menu > Settings menu items.

The following settings are available:

About this CellXpert C170i User Management

View information on the device and licenses Create user management
System Settings Maintenance & Qualification
Configure date, time, and network Configure recurring tasks
Device Settings
Configure sounds, relays, display, and Operation
Records
 Menu area
74 CellXpert® C170i
English (EN)

8.2.1 The About this CellXpert C170i menu item

Abb. 8-2: Available fields

Fig. 8-2: Available fields

Name Serial number

The administrator can enter the device name. The serial number of the device, see the name
plate.
Registration Number
The administrator can enter the inventory Software Version
number of the device. User software version
Location Hardware Configuration
The administrator can enter the location of the View information on optional extensions.
device.
License Information
Article Number View information on licenses.
The article number of the model.

 Tap the Menu > Settings > About this CellXpert

C170i menu items.
 Menu area
CellXpert® C170i 75
English (EN)

8.2.2 The System Settings menu item

Abb. 8-3: Available settings

Fig. 8-3: Available settings

Date & Time Install Software Update from USB Drive

Set the date, time, and time zone.
Network
Set the parameters for network operation.

 Tap the Menu > Settings > System Settings menu

items.

8.2.2.1 Date & Time – Setting the date and time automatically

Prerequisites
• The device is connected to the network.
• A time server is available.

If you are connected to the VisioNize Lab Suite, use the automatic date and time setting to
avoid asynchronism.

Changing the date, time, or time zone may temporarily affect the appearance of the chart. The
log may be incorrectly sorted.
1. Tap the Menu > Settings > System Settings > Date
& Time menu items.
2. Switch on the Automatic date & time switch.
3. Tap the Select timezone menu item.
4. Select the continent.
5. Select the time zone.
6. Close the selection.
The Date & Time menu appears.
 Menu area
76 CellXpert® C170i
English (EN)

8.2.2.2 Date & Time – Setting the date and time manually

If you set the date and time incorrectly, some features of the VisioNize Lab Suite might not
work, e.g., Events.

Changing the date, time, or time zone may temporarily affect the appearance of the chart. The
log may be incorrectly sorted.
1. Tap the Menu > Settings > System Settings > Date
& Time menu items.
2. Switch off the Automatic date & time switch.
The Set date and Set time menu items become
active.
3. Tap the Set date menu item.
4. Set the current date.
5. Tap the Confirm button.
The date is saved.
The Date & Time menu appears.
6. Tap the Set time menu item.
7. Set the time.
8. Tap the Confirm button.
The time is saved.
The Date & Time menu appears.
9. Tap the Select timezone menu item.
10. Select the continent.
11. Select the time zone.
12. Close the selection.
The Date & Time menu appears.

8.2.2.3 Network

An Internet connection is not needed for the operation. The operator is responsible for the data security
when connecting the device with the Internet.

Only a network administrator is allowed to connect the device with an internal network or the Internet.

Check the settings for the internal network or the Internet before connecting the device.
 Menu area
CellXpert® C170i 77
English (EN)

Abb. 8-4: Available settings

Fig. 8-4: Available settings

IP Addresses Manual Setup

Current IP address of the device Enter the network settings manually. Active when
the Enable DHCP switch is switched off
MAC Addresses
Address by which the device can be uniquely Self Signed Certificate
identified in the network Generates an own certificate
Enable remote access Enable OPC/REST access
Allow device communication with external
software
Enable DHCP
The device uses an IP address that was assigned
by a DHCP server

1. Tap the Menu > Settings > System Settings >

Network > Manual Setup menu items.
2. Deactivate Enable DHCP with the switch.
The Manual Setup button appears.
3. Tap the Manual Setup button.
4. Enter and confirm your entry.
5. Tap the Back button.
The data is saved. The window for the network
settings appears.

6. Deactivate Enable DHCP with the switch.

The Manual Setup button appears.
7. Tap the Manual Setup button.
8. Enter and confirm your entry.
9. Tap the Back button.
The data is saved. The window for the network
settings appears.
 Menu area
78 CellXpert® C170i
English (EN)

8.2.2.4 Install Software Update from USB Drive

1. Download the update from the website www.eppendorf.com/software-downloads/ .

2. Unzip the update file at the top level directory of a USB drive.
The device has only access to data at the top level of the USB drive.
• Do not save the data in a folder.
3. Tap Menu > Settings > Install Software Update from USB Drive.
4. Insert the USB drive into the USB port of the device.
An installation dialogue appears on the screen.
5. Confirm the installation.
The device will prepare the update and then reboot.
6. Wait for the update file to be copied to the device.
7. Unplug the USB drive to start the installation process.
Do not abort the software update. If the update is stopped, this may result in loss of data and
the device will have to be reset to the factory settings. If this happens contact the authorized
service.

8.2.3 The Device Settings menu item

Abb. 8-5: Available settings

Fig. 8-5: Available settings

Acoustic Signals Relays Settings

Settings for the audible alarm and the volume Configuration of the BMS relay contact alarm
Display Settings Gas System
Settings for the brightness of the display Configuration of the low pressure monitor
Home Screen Configuration Operation Records
Configuration of the home screen Configuration of the Operation Records template

1. Tap the Menu > Settings > Device Settings menu

items.
 Menu area
CellXpert® C170i 79
English (EN)

8.2.3.1 Acoustic Signals – Setting the audible alarm

Due to safety reasons, it is not possible to totally disable the alarm signals.

1. Tap the Menu > Settings > Device Settings >

Acoustic Signals menu items.
2. To activate the interface signals incl. door alarm,
tap the Alarms and Interface button.
3. To change the volume of the interface signals,
swipe the slider.
4. To check the volume of the alarms or interface
signals, tap the respective Test button.
5. To activate only the alarm signals (all notifications
from the information bar like errors, alarms, and
warnings), tap the Alarms button.

8.2.3.2 Display settings – Setting the touch screen

1. Tap the Menu > Settings > Device Settings >

Display Settings menu items.
The slider for Display Brightness is displayed.
2. To change the brightness of the display, swipe the
slider.

8.2.3.3 Home Screen Configuration – Configuring the home screen

1. Tap the Menu > Settings > Device Settings > Home
Screen Configuration menu items.
You can specify which functions appear on the
home screen. You can set the order of the
functions on the home screen. You can choose
the following functions:
• Temperature
• CO2 Concentration
• O2 Concentration (option)
• Relative Humidity (option)

2. For a function to appear on the home screen,

activate the switch.
 Menu area
80 CellXpert® C170i
English (EN)

3. Set the order of the functions on the home screen

using the arrow keys.
On the home screen, the functions appear in the
same order as in the list.
• If 2 areas are not occupied on the home screen,
the event log appears on the home screen.
• If 4 areas are not occupied on the home screen,
the event log and chart appears on the home
screen.

If the O2 sensor is temporarily not needed, deactivate the switch. After activating the switch,
the sensor needs some time to reinitialize.

8.2.3.4 Relay settings – Configuring the BMS relay contact alarm

You can choose events for the 4 relays of the device. A signal is transmitted to the respective relay if a
chosen event occurs. The relays can be connected to a BMS.

1. Select the relay you want to edit.

2. Tap on the black pen of the relay.
An overlay appears with events to select for each relay:
• Temperature alarm
• Temperature high alarm
• Temperature low alarm
• CO2 alarm
• CO2 high alarm
• CO2 low alarm
• O2 alarm (option)
• O2 high alarm (option)
• O2 low alarm (option)
• Humidity alarm (option)
• Water level alarm (option)
• Warnings
• Error
• Any alarm/Warning/Error
• Off

3. Select an event.

The selected relay transmits a signal, if there is an event happening.

 Menu area
CellXpert® C170i 81
English (EN)

8.2.3.5 Relay settings – Test Relay

1. Tap the Menu > Settings > Device Settings > Relay
Settings menu items.
2. Tap the Test Relay button.
The relay is active for 15 seconds to check the
correct connection to the BMS.

8.2.3.6 Gas system – Pressure Monitor

If applications need low or very low pressure, switch off the pressure monitor for low gas pressure to
prevent warnings and errors to be generated.

1. Tap the Menu > Settings > Device Settings > Gas
System menu items.
2. To deactivate the pressure monitor, slide the
switch to the O position.

Without the monitoring of the low pressure, other messages may occur. Since the gas system
might work beyond the specified conditions (0.05–0.15 MPa), timeout warnings for the gas
concentration may occur, followed by error messages.

8.2.3.7 Operation Records Settings

1. If you want to see your company name on all exported documents of the Operation Records, tap the
Menu > Settings > Device Settings > Operation Records Settings menu items and enter your company
name.
 Menu area
82 CellXpert® C170i
English (EN)

8.3 Alarms

Alarms can be triggered for several conditions.

• Door alarm: triggers if the outer door has been open for too long.
• Temperature alarm: triggers if the temperature in the interior exceeds the alarm limits.
• CO2 alarm: triggers if the CO2 concentration in the interior exceeds the alarm limits.
• O2 alarm (option): triggers if the O2 concentration in the interior exceeds the alarm limits.
• Relative humidity alarm (option): triggers if the humidity in the interior deceeds the alarm limits.
• Water level alarm (option): triggers if the filling level of the water tray is below 0.5 L.

An alarm message appears in the information bar if a value is out of a specified range of setpoints. The
alarm is active until the value is within the set alarm limit again. The alarm system is paused for a defined
time span so that no unnecessary alarm messages are triggered after:

• switching on the incubator

• changing a setpoint
• closing the outer door
• high-temperature disinfection
 Menu area
CellXpert® C170i 83
English (EN)

8.3.1 Calling up the alarm overview

• Tap the Menu > Alarms menu items.

Abb. 8-6: Overview with device alarms, warning limits and alarm limits

1 2 3 4

Fig. 8-6: Overview with device alarms, warning limits and alarm limits

1 Lower alarm limit 3 Alarm activated (temperature)

2 Upper alarm limit 4 Alarm not activated (CO2)
 Menu area
84 CellXpert® C170i
English (EN)

If there is an active alarm, the exceeded alarm limit is highlighted in red.

8.3.2 Setting alarms and alarm limits

When delivered the factory set alarm limits are:

• Temperature: ±0.5 K
• CO2 and O2: ±0.5 %
• RH: 80 %
• Door: 30 s
• Water level: on

You can set the alarm limits closer to the setpoint. Alarm messages may occur more frequently
then.

If you change a setpoint, the alarm limits change relatively.

1. Tap the Menu > Alarms menu items.

2. To call up an alarm, tap the corresponding line.
 Menu area
CellXpert® C170i 85
English (EN)

1 2 3 4

1 High alarm 4 Actual value

2 Low alarm 5 Activate or deactivate alarm
3 Sets the alarm limits around the setpoint in line
with factory settings

The window for the alarm settings appears.

3. To change an alarm limit, tap the alarm limit.
 Menu area
86 CellXpert® C170i
English (EN)

The number pad appears.

4. Enter the new alarm limit.
5. Confirm your entry.
The entered alarm limit appears on the display.
 Menu area
CellXpert® C170i 87
English (EN)

8.4 Events

This area contains messages and alarms with the corresponding time and, if applicable, the user. You can
filter and export Events.

Abb. 8-7: The Event log screen

1 2 3 4

Fig. 8-7: The Event log screen

1 List with all notifications and messages 3 Filter options: This button is highlighted in blue
if messages are filtered.
2 Confirm all notifications
4 Export list with messages to a USB storage
medium

Up to 100 000 notifications and messages can be saved. The oldest entries are overwritten if there are more
than 100 000 entries.
 Menu area
88 CellXpert® C170i
English (EN)

8.4.1 Retrieving events

1. Tap the Menu > Events menu items.

8.4.2 Filtering events

1. Tap the Filter button

2. Select the filter. If a filter is enabled, a checkmark appears next to the filter. The Filter symbol is
highlighted in blue.
3. To disable all filters, tap the Clear all filters button.

8.4.3 Calling up more information

1. Select a message or notification from the event list.

A window with further information appears.
2. To navigate to messages or notifications, use the arrow keys.

8.4.4 Exporting events

Currently filtered event logs are exported.

1. Plug in a USB storage medium.

2. To export Events as a list, tap the Export button.
3. To finish the procedure, acknowledge the notification.
 Menu area
CellXpert® C170i 89
English (EN)

8.5 Charts

The chart shows 2 functions on 2 y-axes. The time is shown on the x-axis.

The data of the last 7 days is stored uncompressed. Data older than 7 days up to 6 months is
stored compressed. The compression of the data may affect the appearance of the chart.
Abb. 8-8: Chart screen

Fig. 8-8: Chart screen

1 Select the function 3 Name of the function of the displayed values

The chart displays the values of the selected
4 Export chart data to a USB storage medium
function.
2 Select the time span
 Menu area
90 CellXpert® C170i
English (EN)

8.5.1 Opening the chart

1. Tap the Menu > Charts menu items.

8.5.2 Selecting functions

1. To select which function is shown, tap the correspondent button.

A window with 2 lines appears.

2. To select the function for the left y-axis, tap the upper line.
 Menu area
CellXpert® C170i 91
English (EN)

3. To select the function for the right y-axis, tap the lower line.

A window with the available functions appears.

4. Select the function.
The function appears in the tapped line.
5. Confirm your selection.
The functions appear in the chart.

8.5.3 Selecting the time span

1. To select the time span, tap the correspondent button.

2. Select the time span.

The time span appears on the x-axis.

The program measures the data of a function every 10 seconds. If you select a large time span, the
resolution of the displayed data is adjusted.
 Menu area
92 CellXpert® C170i
English (EN)

8.5.4 Displaying values from the chart

1. To display numerical values from the chart, select a defined time.

2. Tap the corresponding point in the chart. The functions appear in a separate window.

8.5.5 Exporting the chart

1. To export the data from the chart, tap the Export button.
The currently selected time span and the currently selected parameters are exported.

8.6 Export

You can export charts, logs and data for service to a USB storage medium.

1. Connect a USB storage medium.

2. Tap the Menu > Export menu items.
A list for the export appears.
3. Select which data to export.
Data shown in gray will not be exported.
4. Tap the Export button.
The data is stored. The notice Export successful
appears if the export is finished.
5. Confirm the export.
6. Remove the USB storage medium.
 Menu area
CellXpert® C170i 93
English (EN)

8.7 Recurring tasks

The CellXpert C170i features the option of activating an automatic reminder for common tasks. Eppendorf
provides a set of predefined tasks with the delivery of the incubator. The user can edit existing tasks or
define new tasks.

8.7.1 Predefined tasks

Task name Description

Routine maintenance by epServices Tasks offered by Eppendorf: If you want
maintenance to be executed regularly, contact your
local Eppendorf partner.
High-temperature disinfection Tasks assisted by the operation of the incubator.
Performance check
Chamber cleaning Tasks executed manually and independently from
Water refill the operation software.

8.7.2 Perform a recurring task

1. Tap the Menu > Maintenance & Qualification > Recurring Tasks menu items.

A list with predefined tasks is displayed.

2. Select a task.
 Menu area
94 CellXpert® C170i
English (EN)

3. Tap the Perform task button.

If you select the High Temperature Disinfection task, a software-guided procedure starts.

If you select the Performance Check tasks, a software-guided procedure starts.

If you select other tasks, you can confirm the execution of the tasks.

Only an authorized service technician can execute tasks offered by Eppendorf.

The entry of the last execution of a task is updated, when this task is executed again.

A warning gives the user a hint, that a task has to be executed. To define an interval for the warning, see
Edit a recurring task.

8.7.3 Edit a recurring task

You can edit an existing task.

1. Tap the Menu > Settings > Recurring Tasks menu items.

A list with predefined tasks is displayed.

2. Select a task.
 Menu area
CellXpert® C170i 95
English (EN)

3. Tap the Edit button.

A wizard window appears that explains the task.

4. Tap the Continue button.
 Menu area
96 CellXpert® C170i
English (EN)

5. Select to have the incubator warn you when the task is overdue. When selecting Off, you can still define
an interval without a warning.
6. Tap the Continue button.

7. Define the interval: yearly, monthly, weekly, or daily. Additionally you can set a reminder.
8. Tap the Finish button.

8.7.4 Define a new recurring task

To set a customized reminder, you can define a new task. Tasks that have been defined by yourself are
deletable.
 Menu area
CellXpert® C170i 97
English (EN)

1. Tap the Add Task button.

A wizard window appears.

2. Enter the task name

 Menu area
98 CellXpert® C170i
English (EN)

3. Tap the Next button.

4. Select to have the incubator warn you when the task is overdue. When selecting Off, you can still define
an interval without a warning.
5. Tap the Continue button.
 Menu area
CellXpert® C170i 99
English (EN)

6. Define the interval: yearly, monthly, weekly, or daily. Additionally you can set a reminder.
7. Tap the Finish button.

8.8 Operation Records

All experiments are documented and stored. If there are more than 1000 entries, the oldest
entries are overwritten.

8.8.1 Accessing Operation Records

1. Tap the Menu button.

2. Tap the Operation Records button.
The list of experiments that have been completed is displayed.

8.8.2 Creating an experiment

1. Tap the + button.

2. Enter the record name for the experiment.
3. Enter the start date.
4. Enter the finish date.
5. Confirm the entries.

8.8.3 Filtering entries

1. Tap the button for the filter options.

The Set Filters window with the following filter options opens:
• Result: filter by result All Records, Record contains warnings, Record contains alarm or Record
contains error
• From date: filter from a date
• Until date: filter up to a date
• Operation name: filter by operation name
• User: filter by user if user management has been set
In this window, entries are confirmed with the Confirm button or reset with the Reset Filters button.
2. Assign values to the required filter options and tap on Confirm.
The filtered entries are displayed.

8.8.4 Exporting entries

1. Connect a USB stick and tap the Export button.

The entries are saved in a PDF document. If the export was successful, the Export successful window
appears.
 Menu area
100 CellXpert® C170i
English (EN)

8.8.5 Displaying information on an experiment

1. Tap the desired list entry.

Information on the selected experiment is displayed and can also be exported.
• Result: The result Successfully Created, Record contains warnings, Record contains alarm or Record
contains error is displayed.
• Initial Parameters: The set temperature, CO2 concentration, O2 concentration including alarm limits,
and RH alarm limit are displayed.
• Time Span: The start time and end time are displayed.
• Events: Event information is displayed.
• System: The system information is displayed.

You can define your company name for the Operation Records (see Operation Records Settings on p. 81).

8.9 Screen Lock

You can lock the touchscreen to avoid inadvertent changes during operation.

8.9.1 Locking the touchscreen

 Tap the Menu > Screen Lock menu items.

 Menu area
CellXpert® C170i 101
English (EN)

8.9.2 Unlocking the touchscreen

 Press and hold Unlock Screen until the touchscreen becomes unlocked.

8.9.3 Activating or deactivating the Automatic Screen Lock

1. Tap the Menu > Settings > Device Settings > Screen
Lock menu items.

2. To activate or deactivate the Automatic Screen

Lock tap the status button.

3. If you activate the Automatic Screen Lock, you can

adjust the time for the Screen Lock to come on by
tapping Set Timer.
 Menu area
102 CellXpert® C170i
English (EN)
 User administration
CellXpert® C170i 103
English (EN)

9 User administration
9.1 The user administration concept

The user administration can be used to organize access to the incubator. There are three user roles:

• Administrator
• User with standard rights
• User with restricted rights

9.1.1 User roles for working with user administration

Administrator (role with extra rights)

• Configuring the incubator
• Access to user administration

User with standard rights

• A normal user can operate the incubator without restrictions.

User with restricted rights

• A user with restricted rights can operate the incubator with certain restrictions, i.e. acknowledge
notifications and view settings.

Logged out user

• A logged out user can view all relevant information.

9.1.2 Working without user administration

Without user administration, all users have the same rights as an administrator.
 User administration
104 CellXpert® C170i
English (EN)

9.1.3 User rights

Tasks User with restricted User with standard Administrator/user

rights rights (rights without user
administration)
Change parameters x x
Change alarm limits x
Change settings x
Change relay settings x
Change alarm volume x x
Change home screen x x
configuration
Change own PIN/ x x x
password
Change user name x
Change user rights x
Acknowledge alarm x x
Acknowledge error x
Acknowledge warnings x x x
Start high-temperature x x
disinfection
Start performance check x
Start relay test function x
Export event log x x x
Export charts x x x
Login/logout x x x
View administrator x x x
settings
View user settings x x x
View device information x x x
Configure network x
settings
Create user account x
Reset user password x
Delete user account x
 User administration
CellXpert® C170i 105
English (EN)

9.2 Establishing the user management

NOTICE! Data loss due to loss of the administrator password

The administrator is only able to change his password or PIN using his access data. No
changes will be possible in the user administration or the system settings if the
administrator's access data is lost.
In this case, the device will have to be reset to the factory settings by an authorized service
technician. All user accounts and data and settings stored on the device will be deleted.

 Create a second user account with administrator rights.

 Keep the administrator password secure.

9.2.1 Creating an administrator

Prerequisites
• The incubator is ready to operate.
• The home screen is active.

1. Tap the Menu button and navigate to the Settings

> User Management menu item.
2. To activate the user administration, slide the User
Management switch to the I position.
The Login mode window appears.

3. Define the login mode for all users.

4. Continue the process with Continue.
The Administrator credentials window appears.

5. Enter the user name for the first administrator in

the Enter User Name field.
6. Continue the process with Continue.
The Administrator credentials window appears.
 User administration
106 CellXpert® C170i
English (EN)

7. Enter PIN or password in the Enter PIN/Password

field. Confirm the entry in the Repeat PIN/
Password field.
8. Continue the process with Continue.
The User Management successfully enabled
window appears.
The user administration is activated.
The user account for the first administrator is
created.

9. Confirm the message.

The User Management window appears.
It is now possible to edit the user administration.

9.2.2 Editing the user administration

Prerequisites
• The incubator is ready to operate.
• The administrator is logged in.
• The home screen is active.

1. Tap the Menu button and navigate to the Settings

> User Management menu item.

2. Define the settings for the user administration.

 User administration
CellXpert® C170i 107
English (EN)

• User Management: Switch the user administration on and off.

• Automatic Logout: Define the time after which a user is automatically logged out if the touch screen is
not used.
• Login Mode: Define the login mode for all users.
• Grant all users extra privileges: Restricted rights (Restricted User) or standard rights (User) are active for
all users.

When the Grant all users extra privileges option is activated, a login is not necessary. Also,
users who are not recorded in the user administration can operate the device with these rights
(Restricted User / User).

9.2.3 Deactivating the user administration

NOTICE! Data loss in case of deactivation of the user administration

Deactivating the user administration deletes all user accounts.

 Check whether deactivation of the user administration is required.

 Inform all users that the user accounts have been deleted.

Prerequisites
• The incubator is ready to operate.
• The administrator is logged in.
• The home screen is active.

1. Tap the Menu button and navigate to the Settings

> User Management menu item.
2. To deactivate the user administration, slide the
User Management switch to the 0 position.
The Disabling User Management window appears.

3. Continue the process with Continue.

The Confirm deleting all user accounts window
appears.
 User administration
108 CellXpert® C170i
English (EN)

4. Enter the PIN/password.

5. Complete the process with Confirm.
The user administration is deactivated. All user
accounts are deleted.

9.3 User login

Prerequisites
The user administration is set up, and you can login as a user or administrator.

Abb. 9-1: The Login screen

1 2

4 3

Fig. 9-1: The Login screen

1 Edit box 3 Abort login

2 Number pad 4 Delete the entry step by step

Logging in as a user
1. Tap the Menu > Login menu items.
2. Enter your user ID/user name and your PIN/password.
If the correct PIN or password was entered, the user is automatically logged in.
 User administration
CellXpert® C170i 109
English (EN)

Logging out as a user

1. Tap the Menu > Logout menu items.

9.4 Editing user accounts as an administrator

NOTICE! Data loss due to unauthorized use of the administrator password.

The administrator password protects the device software from unwanted access.

 Make a note of the administrator password.

 Store the administrator password in a secure location.
 Only provide the administrator password to individuals who work with the system
configuration.
 Please contact Eppendorf SE if you have any problems with the administrator password.

9.4.1 Creating a user account

You can create 999 user accounts.

Prerequisites
• The administrator is logged in.

1. Tap the Menu button and navigate to the User

menu item.
The list with the user accounts is displayed.
2. Tap the Add User button.
The Enter User Name for the new user field
appears.

3. Enter the user name.

4. Confirm your entry.
The User credentials window appears.
The user account is created. The user data
appears in the window.
The user is assigned to the user group Restricted
User.
 User administration
110 CellXpert® C170i
English (EN)

5. Optional export of user data: Connect a USB

storage medium and tap the Export button.
6. Confirm the export.
The user data is exported to the USB storage
medium in a TXT file.
7. If the export is successful, the Export successful
window appears.
To complete the process, confirm the message.

9.4.2 Editing user accounts

Users with restricted user rights or standard rights can only edit their own Full Name and
E-mail entries. Users can always change their own PIN or password.
Administrators can assign a new user ID to a user account and change the rights.

Prerequisites
• The administrator is logged in.

1. Tap the Menu button and navigate to the User

menu item.
The list with the user accounts appears. Entries
that can be edited are indicated by a black pen.
2. Select the user account.
3. Optional: Enter the full name.
4. Optional: Enter the e-mail address.
5. To change the user ID, tap User-ID.
6. Select the new user ID.

7. To change the user group and the corresponding

rights, tap Role.
The list with the available user groups appears.
8. Assign a user group to the user.
• Restricted User
• User
• Administrator
The selected parameters are stored and appear in
the user account.
 User administration
CellXpert® C170i 111
English (EN)

9.4.3 Deleting a user account

Prerequisites
• The administrator is logged in.

1. Tap the Menu button and navigate to the User

menu item.
The list with the user accounts appears.
2. Select the user account to be deleted.
3. Tap the Recycle bin symbol.
The Confirm the deletion of: window appears.

4. Confirm that you want to delete the user account.

The user account is deleted.

9.4.4 Resetting the password/PIN for a user account

If a user has forgotten their password/PIN, the administrator can generate a new password.

The administrator can only change their password or PIN with their current access data. If the
administrator’s access data is lost, changes are no longer possible in the user administration.
In this case, the device must be reset to the factory settings by an authorized service
technician. All user accounts and the programs, log files, and logs stored on the device will be
deleted.

 Create a second user account with the administrator rights.

Prerequisites
• The administrator is logged in.

1. Tap the Menu button and navigate to the User

menu item.
The list with the user accounts appears.
2. Select the user account.
3. Tap the Reset Password/PIN button.
The Do you want to reset the Password/PIN for:
window appears.
 User administration
112 CellXpert® C170i
English (EN)

4. Confirm the process with Reset.

The New Credentials window appears.
The new password/PIN is created automatically.

5. To export the user data, connect the USB storage

medium and tap the Export button.
6. Confirm the export.
The user data is exported to the USB storage
medium in a TXT file.
7. If the export is successful, the Export successful
window appears.
To complete the process, confirm the message.

9.5 Administrating your own user account

Users can only edit their own Full Name and E-mail entries. Users can always change their own PIN or
password.

Prerequisites
• The user is logged in.

1. Tap the Menu button and navigate to the User

menu item.
The list with the user accounts appears. Entries
indicated by a black pen can be edited.
2. Select the user account.
3. Optional: Enter the full name.
4. Optional: Enter the e-mail address.
5. To change the password/PIN, tap the Change
Password/PIN button.
6. Enter the current password/PIN in the Enter
current Password/PIN field.
7. Enter the new password/PIN in the Enter new
Password/PIN and Repeat new Password/PIN
fields.
8. Confirm your entry.
The message Password/PIN successfully changed
appears.
The new password or the new PIN is active.
 Maintenance
CellXpert® C170i 113
English (EN)

10 Maintenance
10.1 Routine maintenance
10.1.1 General

WARNING! Risk of injury to personnel or damage to equipment!

 Do not move 2 stacked incubators for maintenance or cleaning.

WARNING! Risk of injury to personnel or damage to equipment!

 While moving the incubator, ensure that the door is closed.

 Two trained persons are needed for moving an incubator.
 Risk of the device falling due to its high center of gravity.

WARNING! Risk of injury to personnel or damage to equipment

 While cleaning or disinfecting/decontaminating the chamber, take care not to break small
sensors/other parts or destroy your protective gloves by wiping with too much force.

To ensure that chamber conditions remain stable, minimize the length of time that the door is open. When
you open the door, wipe off any condensation that may have formed at the inner seal to avoid condensation
build-up.

10.1.2 Daily checks

1. Check if the temperature level and the CO2 level are within the specification.
2. Check the reserve pressure in the CO2 cylinder (normally 725 psi (50 bar) when full). The design of the
incubator ensures low consumption of CO2. If there is a significant drop at the cylinder pressure, it
means that the cylinder is almost empty and should be replaced. Make sure that there are no leaks at
any of the connections. Checking the pressure and the connections helps to increase the lifetime of the
CO2 supply and to avoid accidentally running out of CO2.
3. Clean any spills in the chamber immediately.
4. Routinely check the screen for any alarms or events that may have occurred.

10.1.3 Weekly checks

Clean and refill the water tray with an appropriate amount of warm, distilled, sterile water. The use of warm
water ensures a rapid return to optimum chamber conditions.

10.1.4 Monthly checks

Clean the exterior and interior of the device.

 Maintenance
114 CellXpert® C170i
English (EN)

10.1.5 Yearly checks

Let the sensors be maintained by a qualified service technician.

Exchange the in-line gas filter.

10.2 Performance check

The performance check cannot replace a verification by Eppendorf Service.

Consider the accuracy and the measuring principle of the measuring instrument.
Check the accuracy of the measuring instrument, if there is a discrepancy between the
measured values and the displayed values. Exact limits are not definable for the values as they
depend on the measuring method.

If the measurement is reliable and the measuring instruments are suitable:

• Export the measuring result
• Contact your local Eppendorf partner

The performance check is used to check the accuracy of temperature and gas concentration with an
external measuring instrument. A programmable interval reminds you of the need to run a performance
check.

During a performance check the values, that were set by the user and that are not to be checked, do not
have to be modified. The device has default settings. Only the value you want to check will be switched to
its default setting.

Default settings
• Temperature: 37 °C
• CO2: 5 %
• O2: 10 %

If your samples tolerate the default setting of a value to be checked, they may remain in the
device. If not, take the samples out of the device.

Performance check for temperature

• If your cells are growable at 37 °C, samples may remain in the device during the performance check. If
not, take the samples out of the device.

Performance check for CO2

• If your cells are growable at 5 % CO2, samples may remain in the device during the performance check.
If not, take the samples out of the device.

Performance check for O2

• If your cells are growable at 10 % O2, samples may remain in the device during the performance check.
If not, take the samples out of the device.
 Maintenance
CellXpert® C170i 115
English (EN)

Prerequisites
• External measuring instruments are available.
• The incubator is ready to operate.
• The home screen is displayed on the touch screen.
• If using a user administration, the administrator has to be logged in.
1. Tap the button Menu on the control panel.
2. Tap the button Maintenance & Qualification.
3. Tap on Recurring Tasks.
4. Tap Performance check temperature, Performance check CO2 or Performance check O2.
Abb. 10-1: The guided procedure starts

Fig. 10-1: The guided procedure starts

5. Tap the button Continue.

Use a calibrated measuring instrument. Consider the specification of the measuring
instrument, particularly the operating conditions, and measurement accuracy.
 Maintenance
116 CellXpert® C170i
English (EN)

6. Specify the external measuring instrument by entering its name. Enter the last calibration date of the
measuring instrument.
Abb. 10-2: Documentation of the verification run

Fig. 10-2: Documentation of the verification run

 Maintenance
CellXpert® C170i 117
English (EN)

For temperature

To preserve the door seals, use the access port to bring the cable into the chamber.

1. Open the outer and the inner door.

2. Place the measuring instrument at the middle of the second shelf from the top.
3. Close the inner and outer door.
Abb. 10-3: Placement of the external temperature sensor

Fig. 10-3: Placement of the external temperature sensor

For CO2 and O2

Use a thin, but solid tubing with a maximum diameter of 6 mm. Take care that the tubing does
not become damaged.

1. Open the outer door.

2. Conduct the tubing through the sample port.
 Maintenance
118 CellXpert® C170i
English (EN)

3. Close the outer door.

If the displayed value at the analyzer is too low (CO2) or too high (O2), the analyzer cannot
draw the sample correctly. The displayed values from the analyzer and the incubator display
differ.

Solution
• Pull out the blind plug at the rear of the incubator.
• Repeat the measurement.
• Refit the blind plug after drawing the sample.

 Tap the button Start Verification.

Abb. 10-4: Depending on the performance check, temperature or gas concentration are equilibrated. The remaining run time is displayed.

Fig. 10-4: Depending on the performance check, temperature or gas concentration are equilibrated. The
remaining run time is displayed.

 Enter the detected value of temperature or gas concentration at the touch screen.
 Maintenance
CellXpert® C170i 119
English (EN)

Abb. 10-5: Entering the reference value

Fig. 10-5: Entering the reference value

 Confirm the value.

 Tap the button Continue.
 Maintenance
120 CellXpert® C170i
English (EN)

Abb. 10-6: The result of the performance check is displayed

Fig. 10-6: The result of the performance check is displayed

 To store the result on a USB storage medium, tap the button Export.
 Tap the button Confirm.
 Remove the external measuring instrument.
The incubator is automatically reset to the previously used temperature and gas concentration.
 Maintenance
CellXpert® C170i 121
English (EN)

10.3 Exterior cleaning

DANGER! Risk of electric shock from spilled liquid

 Switch off the device and disconnect it from the mains/power supply before starting
cleaning or disinfecting work.
 Do not allow any liquids to penetrate the inside of the housing.
 Do not spray the housing.
 Only connect the device to the mains/power supply, if it is completely dried.

NOTICE! Damage due to aggressive cleaning agent or sharp objects

Incorrect cleaning agents can damage the display, surfaces, and printing.

 Do not use corrosive cleaning agents, aggressive solvents, or abrasive polishes.

 Do not incubate the accessories in aggressive cleaning agents or disinfectants for an
extended period of time.
 Do not use sharp objects to clean the device.

1. Clean the exterior of the incubator by wiping it over with a soft cloth, moistened with soapy water.
2. Wipe the exterior surfaces again with a rinsed cloth.
 Maintenance
122 CellXpert® C170i
English (EN)

10.3.1 Cleaning the touch screen

The recommended cleaning agent for cleaning the touch screen is a solution of 70 % isopropanol
(isopropyl alcohol) and 30 % distilled water. Follow the appropriate safety regulations while you are using
this solution.

1. Tap Menu and then Screen Lock to activate the screen lock.
The touch screen is disabled.

2. Dampen a clean cloth with the alcoholic solution and wipe the touch screen.
3. To unlock the touch screen, press and hold Unlock Screen.
 Maintenance
CellXpert® C170i 123
English (EN)

10.4 Disinfection/Decontamination

WARNING! Danger due to contact with decontamination agent

 Wear protective equipment, gloves, and protective goggles during the cleaning process.
 Wear respiratory protection when aerosolization is suspected.

WARNING! Risk of personal injury or damage to equipment due to spilled infectious

material

 Immediately decontaminate the exterior and the interior of the device, if infectious
material was spilled.

WARNING! Risk of personal injury or damage to equipment

 While cleaning or disinfecting/decontaminating the chamber, take care not to break small
sensors/other parts or destroy your protective gloves by wiping with too much force.

NOTICE! Corrosion due to aggressive cleaning agents and disinfectants.

 Do not use any corrosive cleaning agents, aggressive solvents or abrasive polishes.
 Do not incubate the accessories in aggressive cleaning agents or disinfectants for longer
periods.

NOTICE! Risk of material damage

 Never use any of the following substances to clean the stainless steel, it will result in
damage: sodium azide, aqua regia, iodine, ferric chloride, or sulphuric acid.

NOTICE! Risk of material damage

 Do not spray disinfecting agent inside the chamber. Spraying will lead to sensor damage.

NOTICE! Risk of material damage

 Ensure that no liquid is spilled on the white porous cover of the humidity sensor in the
chamber.
 Maintenance
124 CellXpert® C170i
English (EN)

10.4.1 Preparing the disinfection/decontamination

The recommended disinfecting agent for use with the incubator is a solution of 70 % Isopropanol or
Ethanol with 30 % sterile distilled water.

If you have any further questions regarding the cleaning and disinfection or decontamination
or regarding the cleaning fluid to be used, contact your local distributor. The contact details
are provided on the back of this manual.

10.4.2 Disinfecting the exterior

It is not necessary to switch off and disconnect the device, when cleaning and disinfection are
part of the software-guided process of high-temperature disinfection.

1. Switch off the incubator.

2. Unplug the incubator from the mains/power supply.
3. Dampen a clean cloth with the alcoholic solution and wipe down all external surfaces, taking care to
keep the alcoholic solution from coming into contact with any mains/electrical outlets or assemblies.

10.4.3 Disassembling the interior equipment

1. Remove the water tray from the chamber.

2. Remove the shelves, bottom to top.
3. Remove the tie rod from the shelf racks.
4. Remove the shelf racks.

10.4.4 Disinfecting/Decontaminating the interior

It is not necessary to switch off and disconnect the device, when cleaning and disinfection are
part of the software-guided process of high-temperature disinfection.

1. Switch off the incubator.

2. Unplug the incubator from the mains/power supply.
3. Clean the water tray by rinsing it in sterile water, wiping it down with the alcoholic solution, and then
rinsing it again with sterile water.
To avoid liquid come into contact with the sensor or the sensor connection, wring out the
cloth.
4. Wipe down the inside of the chamber with the alcoholic solution, and leave it to dry completely.
5. Wipe the internal components of the chamber twice with the alcoholic solution. Wipe off excess liquid
and leave it to dry.
6. Wipe the inner door seal with the alcoholic solution, rinse, and leave it to dry.
 Maintenance
CellXpert® C170i 125
English (EN)

10.4.5 Reassembling the interior equipment

1. Place the shelf racks inside the chamber. Ensure that the shelf rack spacers fit snugly to the side walls.

2. To stabilize the shelf rack, install the tie rod.

Do not forget to mount the tie rod. Shelves can wobble and samples can be lost, especially
when using just one or two shelves.
 Maintenance
126 CellXpert® C170i
English (EN)

3. Install the shelves, top to bottom. Ensure that each shelf’s anti-tip groove is properly inserted (faced
downward and to the rear of the incubator) onto each of the shelf rack guides.
4. Refill the water tray. Slide the water tray onto the lowest shelf rack support. Ensure that the water tray is
pushed fully back.

10.4.6 Putting into operation

1. Plug in the incubator and switch it on.

2. To allow conditions to stabilize, leave the incubator on for at least 2 hours (preferably overnight).
 Maintenance
CellXpert® C170i 127
English (EN)

10.5 High-temperature disinfection

WARNING! Risk of personal injury

Burns due to hot surface.

 Do not touch the equipment during the high-temperature disinfection cycle.

 Do not open the doors during the cycle.
 Do not open the doors after a system crash or a mains/power outage during the
high-temperature disinfection.

WARNING! Risk of personal injury

Scalding due to hot water.

 Remove water from the water tray before starting the high-temperature disinfection cycle.

WARNING! Risk of personal injury or damage to equipment

 While cleaning or disinfecting/decontaminating the chamber, take care not to break small
sensors/other parts or destroy your protective gloves by wiping with too much force.

NOTICE! Risk of material damage

 Never let electrical equipment come into contact with the alcoholic solution.

NOTICE! Risk of material damage

 To avoid possible damage to the CO2 sensor due to condensation, never leave water in the
water tray while the incubator is switched off, or when a high-temperature disinfection
cycle is initiated.

NOTICE! Risk of material damage

 Put the protective cover up on the humidity sensor when executing a high-temperature
disinfection.
 Follow the instructions on the touch screen.

When using the high-temperature disinfection for the first time, it may cause a smell to
develop. Make sure that the location is ventilated.

To work contamination-free, clean and decontaminate the CO2 incubator regularly. The CellXpert C170i
offers the option of activating a reminder that the device is to be decontaminated (see Recurring tasks on
p. 93).
 Maintenance
128 CellXpert® C170i
English (EN)

Prerequisites
The samples have been taken out of the device.

The device is ready to operate.

The protective cover for the humidity sensor is readily available.

The Home screen is displayed on the touch screen.

When using a user administration, a user or an administrator has to be logged on.

1. Tap the button Disinfect.

Abb. 10-7: A software-guided process starts.

Fig. 10-7: A software-guided process starts.

Tap the button Continue. During the period of 14 hours, it is not possible to cultivate cells within the
device.
 Maintenance
CellXpert® C170i 129
English (EN)

2. Follow the instructions on the touch screen.

Tap the button Continue.

3. Follow the instructions on the touch screen.

Tap the button Continue.

 Maintenance
130 CellXpert® C170i
English (EN)

4. For the humidity sensor option: Follow the instructions on the touch screen.

Put the protective cover up on the humidity sensor and tap the button Continue.
5. Follow the instructions on the touch screen.

Tap the button Start Disinfection.

 Maintenance
CellXpert® C170i 131
English (EN)

6. The high-temperature disinfection proceeds in 3 steps: Heating to a disinfection temperature of 180 °C

(heating ramp), holding the disinfection temperature of 180 °C for 2 hours (disinfection) and cooling
down to the preassigned incubation temperature.
Abb. 10-8: Display of the current temperature and estimated time remaining

Fig. 10-8: Display of the current temperature and estimated time remaining

In order to obtain a successful result, keep the incubator doors closed until the
high-temperature disinfection process is completed.
If doors are opened during a high-temperature disinfection, you are asked whether to
continue or to cancel the process.

The estimated time remaining may vary depending on the target temperature and ambient
conditions.
The result of the high-temperature disinfection can be stored on a USB storage medium. Tap
the button Export.
 Maintenance
132 CellXpert® C170i
English (EN)

7. Tap the button Continue.

Abb. 10-9: Display of the result of the high-temperature disinfection after achieving the target temperature

Fig. 10-9: Display of the result of the high-temperature disinfection after achieving the target temperature
8. Follow the instructions on the touch screen.

Tap the button Continue.

 Maintenance
CellXpert® C170i 133
English (EN)

9. If the high-temperature disinfection was successful, tap the button Confirm.

 Maintenance
134 CellXpert® C170i
English (EN)

10.6 Contamination Status

If you have noticed a contamination in the device, you have the chance to inform other users via a message
on the touch screen.

10.6.1 Set the status to Contaminated

1. Tap the Menu > Maintenance & Qualification menu items.

2. Tap on This device is not contaminated.
The window Change Qualification appears.
3. Select Contaminated.
4. Tap the button Confirm.
The information bar displays the Contaminated message.

10.6.2 Reset the status to Not Contaminated

The following steps describe how to reset the status to Not Contaminated.

1. Tap the Menu > Maintenance & Qualification menu items.

2. Tap on This device is not contaminated.
The window Change Qualification appears.
3. Select Not Contaminated.
4. Tap the button Confirm.
The information bar displays the Not Contaminated message for 24 hours.
5. Perform a high-temperature disinfection.
 Troubleshooting
CellXpert® C170i 135
English (EN)

11 Troubleshooting
11.1 General errors

Technical faults may be triggered by faults such as a power failure or power fluctuations. Therefore, it is
sufficient to switch off the device briefly and switch it on after approximately 10 seconds. Check the cable
connections if necessary.

If the suggested troubleshooting measures fail repeatedly, please contact your local
Eppendorf partner. You can find the addresses on the webpage www.eppendorf.com/
worldwide.

Tab. 11-1: Door

Problem Possible cause Solution
Door longer open than (x) Door is open longer than Close the door.
30 seconds. Touch the hint on the display to
switch off the sound.
Set the signal time for door open
signal to another time span: 15,
30, 45, 60 or 90 seconds.
6731FW.50 Long door opening. Door is open longer than Close the door.
Close the door. 5 minutes. Quit the warning note. The
warning note for Door open will
not be shown again.

Tab. 11-2: Temperature

Problem Possible cause Solution
Condensation (Permanent) airflow due to open Relocate the incubator to a more
window or open door or air suitable place.
conditioning device Relocate the air conditioning
device.
Stop the airflow.
Temperature below alarm level Door is opened too long. Check the door opening time.
(x.y °C) Increase the temperature alarm
limit, e.g., 1.0 °C.
Door was opened too often. Check how often the door was
opened throughout a day.
Due to high impact of cold Use a prewarmed medium.
samples, the temperature setpoint Use a smaller number of cold
could not be reached in a specific samples.
time span.
(Permanent) airflow due to open Relocate the incubator to a more
window or open door or air suitable place.
conditioning device Relocate the air conditioning.
Stop the airflow.
 Troubleshooting
136 CellXpert® C170i
English (EN)

Problem Possible cause Solution

Temperature above alarm level Temperature setpoint was To let the incubator cool down,
(x.y °C) decreased without opening doors. open the outer and the inner door.
Increase the temperature alarm
limit, e.g. 1.0 °C.
Due to high impact of hot Use a colder medium.
samples, the temperature setpoint Use a smaller number of hot
could not be reached in a specific samples.
time span.
(Permanent) airflow due to open Relocate the incubator to a more
window or open door or air suitable place.
conditioning device Relocate the air conditioning
device.
Stop the airflow.
Another hot device is too close to Relocate the incubator to a more
the incubator. suitable place.
Relocate the heat emitting device.
Check the distance between the
devices. Increase the distance if
necessary.
A heat emitting device was Take out the heat emitting device.
inserted into the incubator and Increase the upper alarm limit.
generates too much heat.
6731FW.107 Temperature sensor The incubator is located in a cold Let the incubator acclimate at
(XY) is out of range. Check room <10 °C. least 12 h after shipment.
acclimatization and ambient Sensor defect Contact your local Eppendorf
conditions. partner.
6731FW.108 Setpoint not Ambient temperature is too close Change the setpoint to a higher
reached. Cooldown too slow. to the temperature setpoint (less temperature (>=4 °C above
Check environment conditions. than 4 °C difference). ambient temperature).
Ambient temperature is above the Reduce the ambient temperature
temperature setpoint. to >=4 °C to the temperature
setpoint, e.g., by venting the
room.
6731FW.109 X.Y °C not reached Technical fault Restart the incubator.
6731FW.110 X.Y °C not reached Incubator has been started with Restart the incubator.
in time. Check environment cold water.
conditions. Technical fault. Restart the incubator.
 Troubleshooting
CellXpert® C170i 137
English (EN)

Problem Possible cause Solution

6731FW.111 Over-temp detected The incubator features a Change the setpoint to a higher
by Temp limiter. Setpoint not shutdown at 10 K above the temperature (>=4 °C above
reached. Cooldown too slow. setpoint of a heat circuit ambient temperature).
Check environment conditions. according to standard DIN 12880. Reduce the ambient temperature
(Temperature limiter class 1). to >=4 °C to the temperature
setpoint, e.g., by venting the
room.
Restart the device. Increase the
temperature setpoint to >=4 °C
above ambient temperature.
6731W.112 Subnormal The incubator features a Restart the device. Increase the
temperature detected by Temp shutdown at 5 K under the temperature setpoint to >=4 °C
limiter setpoint of a heat circuit above ambient temperature.
according to standard DIN 12880.
(Temperature limiter class 1).

Tab. 11-3: CO2

Problem Possible cause Solution
CO2 Concentration above alarm CO2 set parameter decreased. Open the outer and the inner door
level (x.y%) CO2 cannot pass off the incubator. to let CO2 pass off the incubator.
The alarm limit is too close to the Set the alarm limit to the default
setpoint. limit.
Vessels located in front of the CO2 Relocate the vessels.
sensor.
CO2 Concentration below alarm CO2 set parameter increased. CO2 concentration cannot recover
level (x.y%) in time: Check the pressure
supply.
The alarm limit is too close to the Set the alarm limit to the default
setpoint. limit.
CO2 pressure below warning level CO2 cylinder is almost empty. Check CO2 supply. Exchange CO2
of 0.04 MPa cylinder and adjust gas supply to
0.1 MPa (or range of 0.05 MPa to
0.15 MPa).
Check gas connection on
disconnection or leakage, e.g.,
in-line gas filter, gas connection.
Check flow direction of the in-line
gas filter: Gas supply must be
connected to the INLET side of
the in-line gas filter.
The volumetric flow rate is too Increase the volumetric flow rate,
low. e. g., by opening the CO2 gas stop
valve.
 Troubleshooting
138 CellXpert® C170i
English (EN)

Problem Possible cause Solution

CO2 pressure below alarm level of CO2 cylinder is empty. Check CO2 supply. Exchange CO2
0.02 MPa cylinder and adjust gas supply to
0.1 MPa (or range of 0.05 MPa to
0.15 MPa).
Check gas connection on
disconnection or leakage, e.g.,
in-line gas filter, gas connection.
Check flow direction of the in-line
gas filter: Gas supply must be
connected to the INLET side of
the in-line gas filter.
Ventilate the room, if necessary.
The volumetric flow rate is too Increase the volumetric flow rate,
low. e. g., by opening the CO2 gas stop
valve.
In a short time alternating notice/ CO2 pressure dramatically Check gas connection on
alarm message decreased because of closed disconnection or leakage, e.g.,
CO2 pressure below warning level pressure supply or disconnection/ in-line gas filter, gas connection.
of 0.04 MPa crack of the pressure connection. Check flow direction of the in-line
and gas filter: Gas supply must be
CO2 pressure below alarm level of connected to the INLET side of
0.02 MPa the in-line gas filter.
Ventilate the room, if necessary.
The volumetric flow rate is too Increase the volumetric flow rate,
low. e. g., by opening the CO2 gas stop
valve.
CO2 pressure above alarm level of The CO2 pressure is above the Reduce the CO2 pressure to
0.18 MPa. Check gas supply. alarm level of 0.18 MPa. The inlet 0.1 MPa (14.5 psi, 1 bar) or to the
valve is closed due to the high range of 0.05–0.15 MPa (7.2–
pressure. 21.8 psi, 0.5–1.5 bar). Release the
pressure from the gas tubing that
leads to the incubator. To
disconnect the gas tubing, switch
off the gas pressure, press down
the small ring of the tube
connector, and pull out the gas
tubing.
No CO2 value displayed Initialization sensor is displayed Wait until the incubator is running
after (re-)starting the incubator or in the normal mode.
after HTD.
 Troubleshooting
CellXpert® C170i 139
English (EN)

Problem Possible cause Solution

Setpoint is displayed in red color The CO2 control ran into failure. 1. Tap the CO2 function in the
function area.
2. Restart CO2 control via active
error symbol by tapping the
red button with the X..
The CO2 sensor is defective. Switch off and restart the
incubator.
6731FW213 CO2 control software CO2 control loop took too long Check gas connection on
error or disconnection or leakage, e.g.,
CO2 control loop aborted in-line gas filter, gas connection.
or Check if access port is closed.
CO2 sensor or sensor software Restart the incubator.
Contact your local Eppendorf
defect.
partner.
6731FW214 CO2 control timeout CO2 value error. Check gas connection on
error CO2 setpoint not reached in time. disconnection or leakage, e.g.,
Vessels located in front of the CO2 in-line gas filter, gas connection.
Check if access port is closed.
sensor.
Relocate the vessels.
Too many vessels in the chamber
Restart the incubator.
under extreme conditions.
Contact your local Eppendorf
partner.
Different CO2 errors occur, e. g. Pressure monitor is switched off. Switch on the pressure monitor.
timeout Low CO2 pressure is not Check for warnings or errors.
monitored. Check CO2 supply. Exchange CO2
cylinder and adjust gas supply to
0.1 MPa (or range of 0.05 MPa to
0.15 MPa).
Gas cylinder is empty. Check gas connection on
disconnection or leakage, e. g.,
in-line gas filter, gas connection.
Ventilate the room, if necessary.
 Troubleshooting
140 CellXpert® C170i
English (EN)

Tab. 11-4: O2
Problem Possible cause Solution
No O2 value displayed Initialization sensor is displayed Wait until the incubator is running
after (re-)starting the incubator or in the normal mode.
after HTD.
The O2 option is not available. The O2 option was not ordered.
The O2 option is deactivated. Activate the O2 option.
Setpoint is displayed in red color The O2 control ran into failure. 1. Tap the O2 function in the
function area.
2. Restart O2 control via active
error symbol by tapping the
red button with the X..
The O2 sensor is not plugged in. Switch off the incubator and plug
in the O2 sensor.
The O2 sensor is defective. Switch off and restart the
incubator.
O2 Concentration above alarm The O2 set parameter has been Open the outer and the inner door
level (x.y%) decreased. O2 cannot come into to let O2 stream in the incubator.
the chamber because door is
closed.
The alarm limit is too close to the Set the alarm limit to the default
setpoint. limit.
Vessels located in front of the O2 Relocate the vessels.
sensor.
O2 Concentration below alarm The O2 setpoint has been If the O2 concentration cannot
level (x.y%) increased. build up in time, check the N2
pressure supply.
The alarm limit is too close to the Set the alarm limit to the default
setpoint. limit.
N2 pressure below warning level N2 gas cylinder is almost empty. Check N2 supply. Exchange the
of 0.04 MPa gas cylinder and adjust gas supply
to 0.1 MPa (or range of 0.05 MPa
to 0.15 MPa).
Check flow direction of the in-line
gas filter: Gas supply must be
connected to the INLET side of
the in-line gas filter.
Check gas connection on
disconnection or leakage, e.g.,
in-line gas filter, gas connection.
 Troubleshooting
CellXpert® C170i 141
English (EN)

Problem Possible cause Solution

N2 pressure below alarm level of N2 gas cylinder is empty. Check N2 supply. Exchange the
0.02 MPa gas cylinder and adjust gas supply
to 0.1 MPa (or range of 0.05 MPa
to 0.15 MPa).
Check flow direction of the in-line
gas filter: Gas supply must be
connected to the INLET side of
the in-line gas filter.
Check gas connection on
disconnection or leakage, e.g.,
in-line gas filter, gas connection.
Ventilate the room, if necessary.
In a short time alternating notice/ N2 pressure dramatically Check gas connection on
alarm message decreased because of closed disconnection or leakage, e.g.,
N2 pressure below warning level pressure supply or disconnection/ in-line gas filter, gas connection.
of 0.04 MPa crack of the pressure connection. Check flow direction of the in-line
and gas filter: Gas supply must be
N2 pressure below alarm level of connected to the INLET side of
0.02 MPa the in-line gas filter.
Ventilate the room, if necessary.
N2 pressure above alarm level of N2 pressure above alarm level of Reduce the N2 pressure to
0.18 MPa. Check gas supply. 0.18 MPa. The inlet valve is closed 0.1 MPa (14.5 psi, 1 bar) or to the
due to the high pressure. range of 0.05–0.15 MPa (7.2–
21.8 psi, 0.5–1.5 bar). Release the
pressure from the gas tubing that
leads to the incubator. To
disconnect the gas tubing, switch
off the gas pressure, press down
the small ring of the tube
connector, and pull out the gas
tubing.
6731FW313 O2 control software O2 control loop took too long Check gas connection on
error or disconnection or leakage, e.g.,
O2 control loop aborted in-line gas filter, gas connection.
or Check if access port is closed.
O2 sensor or sensor software Restart the incubator.
Contact your local Eppendorf
defect.
partner.
6731FW314 O2 control timeout O2 value error. Check gas connection on
error O2 setpoint not reached in time. disconnection or leakage, e.g.,
Vessels located in front of the O2 in-line gas filter, gas connection.
Check if access port is closed.
sensor.
Relocate the vessels.
Too many vessels in the chamber
Restart the incubator.
under extreme conditions.
Contact your local Eppendorf
partner.
 Troubleshooting
142 CellXpert® C170i
English (EN)

Problem Possible cause Solution

Different O2 errors occur, e g. Pressure monitor is switched off. Switch on the pressure monitor.
timeout Low N2 pressure is not monitored. Check for warnings or errors.
Gas cylinder is empty. Check N2 supply. Exchange N2
cylinder and adjust gas supply to
0.1 MPa (or range of 0.05 MPa to
0.15 MPa).
Check gas connection on
disconnection or leakage, e.g.,
in-line gas filter, gas connection.
Ventilate the room, if necessary.
Formation of droplets visible No well-balanced ambient Adjust the ambient conditions.
outside the chamber at the front conditions. Increase ambient temperature to
panel >22 °C.
Decrease ambient relative
humidity to <40 %.
Wipe off the droplets.

Tab. 11-5: Humidity

Problem Possible cause Solution
RH below alarm level (x.y%) Door is opened too long. Check the door opening time.
Door was opened too often. Check how often the door was
opened in a day.
Decrease the alarm limit for
humidity to a lower value.
Relative humidity did not reach Confirm the notification and
the alarm limit within 3.5 hours check the RH value again after
after door opening respective some time or change the alarm
5 hours after switching on the limit to a lower value.
device.
The sensor measures wrong Remove the protective cover.
values, because the protective
cover is still covering the humidity
sensor.
No RH value displayed The RH option is not available. The RH option was not ordered.
The RH option is deactivated. Activate the RH option.
Water level hint is displayed Water level is below 0.5 L. Check the water tray weekly.
Refill the water tray or clean and
refill the water tray weekly.
 Troubleshooting
CellXpert® C170i 143
English (EN)

Problem Possible cause Solution

No water level alarm displayed The water level is at its optimum. See Settings > About this CellXpert
C170i and hardware
configuration.
The water level option is not The water level option was not
installed. ordered.
The water level alarm is Activate the water level alarm.
deactivated.
The water level sensor is Switch off and restart the
defective. incubator.
A low water level is not Fill the water tray, if necessary.
recognized during a timeout. The
alarm is disabled for 2 hours after
door closing, 1 hour after
switching on the device.
Water level sensor notifies low Water tray filled with too warm Fill the water tray with water that
although there is water in the water. is a little cooler than the set
water tray. temperature.
6731FW.454 Water level sensor The measured value is out of Restart the incubator.
error range.
No RH alarm The RH alarm is switched off. Switch on the RH alarm.
After a door was opened, it takes Wait until the alarm is armed.
3.5 hours until the relative Do not open the door.
humidity alarm is armed.
Varying RH values Ambient humidity level or water Accept slight deviations. If
level or other factors vary. necessary change the alarm limit
to a lower value.
 Troubleshooting
144 CellXpert® C170i
English (EN)

Tab. 11-6: High-temperature disinfection (HTD)

Problem Possible cause Solution
6731FW.110 180 °C not The water tray is not empty. Switch off and restart the incubator.
reached in time. Check Empty the water tray.
environment conditions Restart the HTD.
Technical fault. Contact your local Eppendorf partner.
HTD can not start with the Water level sensor detects Empty the water tray.
last step of the water.
software-guided process for
HTD
Sensor has been switched off The incubator was restarted Let the incubator cool down to <50 °C
due to high temperature with a too high residual before restarting the HTD.
• CO2 (6731FW.202) temperature after the HTD
• O2 (6731FW.302) has been interrupted.
• RH (6731FW.402)
• Water level
(6731FW.452)
6731FW.750 Error occurred Another error occurred Check previous error messages and follow
during High Temperature during the HTD. the solution table.
Disinfection
6731FW.751 High The incubator has been Restart the HTD.
Temperature Disinfection turned off during the HTD.
interrupted due to power A mains/power failure
loss occurred.
6731FW.753 Door has been Do not open the door during Restart the HTD.
opened during High the HTD.
Temperature Disinfection
 Troubleshooting
CellXpert® C170i 145
English (EN)

Tab. 11-7: General errors

Problem Possible cause Solution
USB stick is not recognized Invalid USB stick Use another USB stick.
Sample is partially dry Shelves are not properly leveled. Level the incubator by placing a
spirit level on a shelf:
• aligned left to right
• aligned front to rear
Add more medium to the cells.
No water in the water tray. Check the water tray weekly.
Refill it or clean and refill it
weekly.
Sample evaporation Low humidity due to empty water Check the water tray weekly.
tray. Refill it or clean and refill it
weekly.
Circular cell growth in cell culture Vibrations from other devices Check if vibration generating
dish result in sample vibrations. elevators or devices like freezers
or centrifuges are too close to the
incubator, see Installation.
Sample contaminated Consistent contamination Perform an HTD.
Contamination of sample Check your sample handling.
Check for contamination during
your application steps.
Condensation at the inner door Water tray filled with too warm Let the water cool down.
after filling of the water tray water. Fill the water tray with water that
is a little cooler than the set
temperature.
Fatal Error – Restart the device Software error Switch off and restart the
displayed on red screen incubator.
Sensor error The initialization of the sensor Restart the incubator.
• CO2 (6731FW.203) failed after switching on the
• O2 (6731FW.303) incubator.
• RH (6731FW.403)
• Water level (6731FW.453)
XY value over range: x.y The measured value is above the Restart the incubator.
or allowed range
XY value under range: x.y or
• CO2 (6731FW.204) the measured value is below the
• O2 (6731FW.304) allowed range.
• Water level (6731FW.404)
 Troubleshooting
146 CellXpert® C170i
English (EN)
 Transport, storage and disposal
CellXpert® C170i 147
English (EN)

12 Transport, storage and disposal

12.1 Transport

CAUTION! Risk of injury from lifting and carrying heavy loads

 At least 4 people are required to safely lift the incubator.

 Use a transport aid for transporting the incubator.

CAUTION! Risk of tipping over

Stacked incubators may topple during transport.

 Do not move 2 stacked incubators.

NOTICE! Damage due to incorrect packing.

Eppendorf SE is not liable for any damage caused by improper packing.

 Only store and transport the device in its original packing.

NOTICE! Damage to the incubator

Lifting the incubator by the door will cause permanent damage to the incubator.

 Lift the incubator only by the lifting handles.

 Never lift the incubator by its door.

12.1.1 Conditions of transport

 Temperature: -25–60 °C
 Relative humidity: 10–95 %
 Atmospheric pressure: 30–106 kPa

12.2 Storage

• Temperature: -25–55 °C
• Relative humidity: 10–95 %
• Atmospheric pressure: 70–106 kPa
 Transport, storage and disposal
148 CellXpert® C170i
English (EN)

12.3 Decontamination before shipment

If you are going to ship the device to the authorized Technical Service for repairs or to your authorized
dealer for disposal, note:

WARNING! Risk to health from contaminated device.

1. Observe the information contained in the decontamination certificate. It is available as a
PDF document on our webpage (https://www.eppendorf.com/decontamination).
2. Decontaminate all parts to be shipped.
3. Include the fully completed decontamination certificate in the shipment.

12.4 Disposal

Observe the relevant legal regulations when disposing of the product.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations
based on EU Directive 2012/19/EU pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business
sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste.
They are marked with the following symbol to indicate this:

As the disposal regulations may differ from one country to another within the EU, please contact your
supplier for more information.
 Technical data
CellXpert® C170i 149
English (EN)

13 Technical data
13.1 Weight/dimensions
13.1.1 Equipment dimensions
Abb. 13-1: Dimensions of CellXpert C170i (without safety latch)

C170i

h d

Fig. 13-1: Dimensions of CellXpert C170i (without safety latch)

Width (w) 71.8 cm (28.3 in)

Height (h) 90.0 cm (35.4 in)
Depth (d) 71.5 cm (28.1 in)
Depth w/o handle/control panel 65.5 cm (25.8 in)
Weight 107 kg (236 lb) – 113 kg (249 lb)
depending on options, w/o accessories

13.1.2 Internal dimensions

Width 53.9 cm (21.2 in)

Height 69.2 cm (27.2 in)
Depth 44.5 cm (17.5 in)
Volume (total) 167 L
Volume (useable, with 4 shelves) 124 L
 Technical data
150 CellXpert® C170i
English (EN)

13.1.3 Floor space required

Width 81 cm (31.9 in)

Depth 60 cm (23.6 in)
Height 95 cm (37.4 in)

13.1.4 Transporting dimensions

Width 120.4 cm (47.4 in), pallet included

Height 117.6 cm (46.3 in), pallet included
Depth 80 cm (31.5 in), pallet included
Weight 126 kg (278 lb) – 132 kg (291 lb)
depending on options

13.1.5 Shelves

Polished stainless steel, perforated (standard)

Width 52.2 cm (20.6 in)

Depth 42.8 cm (16.9 in)
Number of shelves 4 standard; upgrade to 8 shelves
Flatness tolerance of shelf 1.3 mm
Thickness of shelf 1.5 mm
Max. load to the shelf 10 kg
Max. load of the shelf rack 40 kg
 Technical data
CellXpert® C170i 151
English (EN)

13.1.6 Stacked CellXpert C170i

Abb. 13-2: Dimensions of 2 stacked CellXpert C170i (without safety latch)

C170i

d
h1
C170i

h2 h3

h4

Fig. 13-2: Dimensions of 2 stacked CellXpert C170i (without safety latch)

Height Depth Width

h1 h2 h3 h4 d w
194.6 cm 107.3 cm 104.5 cm 14.2 cm 71.5 cm 71.8 cm
(76.7 in) (42.3 in) (41.2 in) (5.6 in) (28.1 in) (28.3 in)
 Technical data
152 CellXpert® C170i
English (EN)

13.2 Power supply

Mains/power connection 100 V – 127 V ±10 %, 50 Hz – 60 Hz

220 V – 240 V ±10 %, 50 Hz – 60 Hz
Power consumption for 110 V – 127 V 1150 W
Power consumption for 220 V – 240 V 1150 W
Overvoltage category II (IEC 61010-1)
Protection class I
Specifications for mains/power cord AC 250V / 16A
3G 1.5 mm2
appliance coupler C19 according IEC 60320-1:2015

13.3 Interfaces
BMS relays 4
BMS max. current 2A
BMS max. voltage 30 V DC/AC
USB 2 x USB 2.0
Ethernet 1 x user
1 x service

13.4 Ambient conditions

Environment For indoor use only.

No wet environment.
Ambient temperature 18–28 °C
Relative humidity 20–80 %, non-condensing
Pollution degree 2
Atmospheric pressure 79.5–106 kPa (use up to a height of 2000 m above
sea level)

13.4.1 Temperature management

Range 4 °C above ambient temperature to 50 °C

Control increment 0.1 °C
Accuracy ±0.4 °C (DIN 12880) at 37 °C and ambient 22 °C
Stability ±0.1 °C at 37 °C and ambient 22 °C
Uniformity ±0.3 °C at 37 °C and ambient 22 °C
 Technical data
CellXpert® C170i 153
English (EN)

13.4.2 High-temperature disinfection

Max. temperature 180 °C / 2 h

Approx. duration 14 h

13.4.3 CO2 control

Range 0.1–20 %
Control increment 0.1 %
Accuracy ±0.3 % at 5 % CO2 at specified relative humidity and at
37 °C and ambient 22 °C
Stability ±0.1 % at 5 % CO2 at specified relative humidity and at
37 °C and ambient 22 °C
Uniformity ±0.1 % at 5 % CO2 at specified relative humidity and at
37 °C and ambient 22 °C
Gas tubing Inner diameter of 6.5 mm and outer diameter of 10 mm
Pore sizes of in-line gas filter 0.2 μm
Sensor type NDIR sensor
Required gas pressure 0.1 MPa (1 bar, 14.5 psi), range 0.05–0.15 MPa (0.5–
1.5 bar; 7.2–21.8 psi)

13.4.4 Humidity

Water tray capacity 2.5 Liters

Humidity 95 % at 37 °C

The relative humidity attained could vary in the range of 92.5 ±2.5 % depending on the ambient humidity
level and other factors.
 Technical data
154 CellXpert® C170i
English (EN)

13.4.5 O2 control

Range 1–20 % or 0.1–20 %, depending on option

Control increment 0.1 %
Accuracy ±0.25 vol% at 37 °C and at specified
relative humidity
Stability ±0.1 % at 37 °C and at specified relative
humidity
Uniformity ±0.1 % at 37 °C and at specified relative
humidity
Gas tubing Inner diameter of 6.5 mm and outer
diameter of 10 mm
Pore sizes of in-line gas filter 0.2 μm
Sensor type ZrO2 (Zirconium dioxide)
Required gas pressure 0.1 MPa (1 bar, 14.5 psi), range 0.05–
0.15 MPa (0.5–1.5 bar; 7.2–21.8 psi)

13.4.6 Calibration

The maximum operating ambient temperature is 28 °C.

Factory calibration of the incubator is carried out at 37 °C, 5 % CO2 and 90–95 % RH, in an
ambient temperature of 20–25 °C with no heat-generating apparatus inside the chamber.

Software calibration adjustments are required to optimize performance if the incubator is being used
outside the operating conditions. Performance specifications are also affected.

Contact Eppendorf Service for advice on calibration adjustments and relevant performance specifications.
Be prepared with the model and serial number of your incubator and the complete details of your operating
conditions.

Performance values represent the average of instruments checked in the factory under optimum conditions.
 Ordering Information
CellXpert® C170i 155
English (EN)

14 Ordering Information
14.1 Shelves and shelf rack
Order no. Description
(International)
6731 070.123 Shelf
for 170 L incubators, 1 piece
6710 859.009 Shelves
for 170 L incubators, 2 pieces
P0628-6390 Shelf rack
for 170 L incubators
P0628-6140 Water tray
for 170 L incubators
6710 859.106 Copper shelves
for 170 L incubators, 2 pieces
P0628-6260 Copper water tray
for 170 L incubators
6731 080.013 Copper kit
copper water tray and 4 copper shelves

14.2 Gas system

Order no. Description
(International)
6731 070.107 Gas tubing
3 m, with in-line gas filter

14.3 Electrics
Order no. Description
(International)
6731 070.069 BMS connector

14.4 Connectivity
Order no. Description
(International)
1006 073.006 Cable
to connect devices with ethernet interface to
VisioNize
VisioNize box, Ethernet, length 5 m
 Ordering Information
156 CellXpert® C170i
English (EN)

14.5 Housing
Order no. Description
(International)
6731 070.034 Plug for access port
2 pieces
6731 070.093 Stacking stand, lower frame
with castors
6731 070.085 Stacking stand, upper frame
for CellXpert C170/C170i
6731 070.158 Universal upper stacking stand
for 170 L incubators
6710 070.235 Safety fastening kit
6731 070.115 Door damper kit
6731 070.166 Door lock
6731 070.140 Protective cover for RH sensor
for high-temperature disinfection

14.6 Accessories
Order no. Description
(International)
P0628-5000 Automatic gas cylinder change-over unit
P0628-6150 New Brunswick™ Galaxy® Gas Analyzer
CO2
P0628-6831 New Brunswick™ Galaxy® Gas Analyzer
CO2, O2
P0628-7890 New Brunswick™ Galaxy® Gas Analyzer
CO2, O2, RH
P0628-7880 Temperature probe 100 mm tip
P0628-7881 Temperature probe 5 mm tip
 Ordering Information
CellXpert® C170i 157
English (EN)

14.7 Upgrade options

The following upgrade options are available:

• Reversal of door opening

• Inner door with 4 door segments
• Inner door with 8 door segments
• O2 option
• Humidity package (water level sensor and humidity sensor)
• Water level sensor
• Humidity sensor
• Copper kit (copper water tray and copper shelves)

Contact your local Eppendorf partner for ordering upgrade options.

 Index
158 CellXpert® C170i
English (EN)

Index Doors .................................................................... 51

A E
Acoustic signal ......................................................79 Error list.............................................................. 135
Administrator ......................................................105 Error message....................................................... 61
Alarm conditions ...................................................82 Error messages ................................................... 135
Alarm limits ...........................................................84 Events ................................................................... 87
Alarm message ......................................................61 Export chart .......................................................... 92
Alarm relay Export data ........................................................... 92
BMS .................................................................40
Exporting event .................................................... 88
Alarm setting .........................................................84
External sensor ..................................................... 54
Ambient conditions .............................................152
Audible alarm ........................................................79 F
Filling quantity...................................................... 53
B
Functions
BMS relay contact alarm .......................................80 Switch functions .............................................. 70

C G
CO2........................................................................49 Gas pressure monitoring ...................................... 81
CO2 control ...........................................................69
H
Connections...........................................................36
High-temperature disinfection ................... 127, 144
Contamination information .................................134
Home screen................................................... 58, 79
D HTD............................................................. 127, 144
Daily checks ........................................................113 Humidity ............................................................. 142
Date .......................................................................75 Humidity sensor.................................................... 50
Device
Register............................................................43 I
Device settings ......................................................78 Inner door latch .................................................... 52
Disinfecting agent ...............................................124 Inspection of boxes............................................... 27
Disinfection/Decontamination.............................123 Internet ................................................................. 76
Display...................................................................58
L
Display settings .....................................................79
Location ................................................................ 30
Disposal ...............................................................148
Login................................................................... 108
Door segment handle ............................................52
 Index
CellXpert® C170i 159
English (EN)

M Utilities requirements ........................................... 29

Menu .....................................................................71
V
Monthly checks ...................................................113
Values
Setting values .................................................. 66
N
Network .................................................................76
W
VisioNize touch enabled device.......................43
Warning message ................................................. 61
O Water level ............................................................ 50
O2 ..........................................................................49 Water tray ............................................................. 53
O2 control..............................................................69 Weekly checks .................................................... 113
O2 sensor ..............................................................38
Y
Outer door handle .................................................51
Yearly checks ...................................................... 114
P
Pressure setting.....................................................38
Protocol .................................................................87

R
Records..................................................................87
Register .................................................................43
Reminder ...............................................................93

S
Safety latch ............................................................34
Settings..................................................................73

T
Task reminder........................................................93
Temperature ..................................................49, 135
Time.......................................................................75
Touch screen .....................................57, 58, 79, 122

U
User administration.....................................106, 107
User requirement ..................................................11
 Index
160 CellXpert® C170i
English (EN)
Certificates
Evaluate Your Manual

Give us your feedback.

www.eppendorf.com/manualfeedback

Your local distributor: www.eppendorf.com/contact

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com