Microbiological Safety Cabinet

BSC-4FA2-HA
User Manual

BIOBASE GROUP

Version 2021.09
 Preface
Thank you very much for purchasing our class II A2 Microbiological Safety Cabinet.
Please read the “1. Precaution” and “4.4 Instructions for Operation” before operating this unit to
assure proper operation. After reading these documents, be sure to store them securely together with
the “Warranty” at a hand place for future reference.

Warning: Before operating the unit, be sure to read carefully and fully understand important
warnings in the operating instructions.

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 Content
Preface.................................................................................................................................................... 1
Content................................................................................................................................................... 2
1. Precautions......................................................................................................................................... 4
2.Warranty.............................................................................................................................................. 7
3. Unpacking, Installation, Debugging...................................................................................................8
3.1 Unpacking................................................................................................................................. 8
3.2 Accessories checking.............................................................................................................. 10
3.3 Installation conditions and using environment........................................................................10
3.4 Installation............................................................................................................................... 11
3.5 Checking after installation.......................................................................................................14
4. User Instructions...............................................................................................................................15
4.1 Functions................................................................................................................................. 15
4.1.1 Product Concept............................................................................................................ 15
4.1.2 Application Range.........................................................................................................15
4.1.3 Working theory/Air flow pattern and protected area.................................................... 15
4.1.4 Protected objects............................................................................................................15
4.1.5 Technical parameters.....................................................................................................16
4.1.6 Performance Index........................................................................................................ 16
4.2 Product structure......................................................................................................................17
4.2.1 Structural composition of BSC-4FA2-HA.................................................................... 17
4.2.2 Structure introduction....................................................................................................17
4.3 Control panel........................................................................................................................... 20
4.3.1 Touch screen display window....................................................................................... 20
4.3.2 Buttons...........................................................................................................................21
4.4. Instructions for Operation...................................................................................................... 22
4.5 Daily maintenance...................................................................................................................22
4.5.1 Preparations before maintenance...................................................................................22
4.5.2 Clean the cabinet surface...............................................................................................23

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 4.5.3 Overall maintenance period...........................................................................................23
4.5.4 Maintenance methods....................................................................................................23
4.6 Methods and procedures for disinfection................................................................................ 24
4.7 Replacement parts list............................................................................................................. 27
4.8 Wiring diagram........................................................................................................................28
5. Trouble shooting and Labels............................................................................................................ 29
5.1 Common faults & solution...................................................................................................... 29
5.1.1 Warning and reminder...................................................................................................29
5.1.2 Trouble shooting............................................................................................................29
5.1.3 Simple accessories replacement.................................................................................... 30
5.2 Label Description.................................................................................................................... 33
6.EMC.................................................................................................................................................. 36

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 1. Precautions

Under all conditions marked with , it is necessary to consult the

document, so as to clarity the nature of potential risks and any

countermeasures that must be taken.

Actions or operations which are prohibited.

Actions or operations which must be followed

Make sure input voltage is correct and stable. The rated load of main power socket should be
higher than cabinet consumption. Plug must be well grounded.

In order to avoid air turbulence, the operator should slightly move his arms during
experiment. Hands should stay inside the working area at least 1 minute before operating. In
order to decrease the times of arms moving into and out of the working area, prepare all the
necessary items inside the cabinet before starting experiment.

Moving principles of different samples inside cabinet: When two or more samples need to be
moved, be sure that low-polluting samples move to high-polluting samples. Movement of
items should also follow the principles of slow-moving.

Samples placed in parallel: Samples should be placed in the cabinet parallel to avoid
cross-contamination between samples and blocking back air grille.

In order to avoid samples being sucked into the negative passage or the blower, do not place
soft and slight samples (for example: soft tissue) on the surface during experiment;

The weight of items placed in the cabinet should be no more than 23kg/(25×25cm2)；

Avoid vibration: avoid using vibration equipment (e.g. centrifuges, vortex oscillator, etc.)
inside the cabinet. Vibration would cause lower cleanliness of operating area and affect
operator protection.

No flame: No flame is allowed inside the cabinet. Using of fire will lead to airflow disorder,
and filter damage. If sterilization is required during the experiment, infrared sterilizer is
highly recommended.

The UV lamp can only be turned on when the front window is closed and the fluorescent
lamp is off.When UV lamp working avoid looking straight.

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ULPA filter life: With the usage time increasing, dust and bacteria accumulate inside ULPA
filter. Filter Resistance is getting bigger, when it reaches the maximum point, there will be
audible and visual alarm. Please replace new ULPA filter, otherwise it will affect the safety
performance of the equipment. The used filter should be processed as medical waste.

There is a negative passage surrounding the work area, which is sealed strictly in the factory.
The operator is not allowed to remove or loose screws of those parts. If necessary, please
contact service personal.

Front Grille is used for air intake and drain. Do not block it, otherwise it will affect airflow.
Armrest is recommended to solve this problem and reducing the operator's wrist fatigue.

Long-term use of Microbiological Safety Cabinets will inevitably cause pollution (e.g. ULPA
filters, corner cabinets, etc.). In order to sterilize thoroughly every 500 hours, formalin
(formaldehyde) fumigation sterilizer is recommended. After sterilization, neutralize
formaldehyde gas with ammonium hydrogen carbonate. Make sure no sterilization gas
escapes during the whole process.

The maximum storage period is one year. If the period is more than one year, performance
test should be done.

In transportation process should take appropriate protective measures according to diagram

on the surface of package. Purchaser in receiving should carefully check the integrity of the
box. if there is damage and crush, please refuse to accept. And timely contact with our
company.

When Microbiological Safety Cabinet installed and used move again, please timely contact
with our company.

The ground bearing requirements: ground bearing capacity ≥350KPa.

Total weight: 145kg.

Allowable pressure of water socket: ≤0.8MPa. (Optional)

For experiments that produce waste liquid, please timely drain wastewater in the sump
through drain valve. If the liquid waste is harmful to human’s body, according to the grade of
pollution should add the corresponding grade container, or connect to the corresponding
grade drainpipes, and drain waste into the experimental equipment to immediately dispose.

Person can operate Microbiological Safety Cabinet only if he is qualified after training,
during using Microbiological Safety Cabinet if it is a harmful experiment, operator must wear
appropriate protective gloves, masks and lab coats, etc., and avoid touching mouth, eyes and

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 face.

There is risk to move the parts moving. Relevant personnel can use this equipment must after
training. while the front window must be raised to 200mm and in this case person can operate
in the cabinet. In this way we can reduce harm out of front window trouble.

When the equipment is repaired or scrapped. The internal parts can’t be thrown about because
of biological hazards. please deal with them according to local regulations.

Serious declaration: we will take no responsibility for risks caused by improper operation
and man-made damages!

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 2.Warranty
1) Warranty is 12 months from EX-factory date (excluding consumable accessories and Fluorescent
lamp, fuse).
2) We will take no responsibility for risks caused by improper operation and man-made damages.
3) After the expiration of warranty, our company is also responsible for repairs, but the
corresponding maintenance cost should be charged.
4) Lifetime of Microbiological Safety Cabinet is 8 years from production date on the label.
5) We can provide equipment drawings and necessary technical data for maintenance companies or
personnel trained by our company.
Warranty declaration: One-year Warranty, Life-long Maintenance

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 3. Unpacking, Installation, Debugging
Please firstly check if packing box is in good condition. If the packing box is damaged, please take
photos.

3.1 Unpacking
Choose the proper unpacking method according to the actual situation.
For wooden box:
1) Method 1 Use M8 Wrench to unpack

Picture 1

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2) Method 1 Necessary tools for unpacking: Electric drill with hexagon dead M8

Picture2
Rapid unpacking diagram (Picture 3). Disassemble the screws shown in the below Picture, then
move the wooden pieces to right and left.

Picture 3

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3.2 Accessories checking
Refer to the packing list and check the accessories.
BSC-4FA2-HA Packing List
Items Model Position Quantity

Main Body Wooden box 1 set

Base Stand Placed in two carton box 1 set

UV Lamp (T6 30W) Top or back of main body 1 pc

Protective fuse(12.5A,250V) 2 pcs

Protective fuse(8A,250V) 1 pcs

Protective fuse(6.3A,250V) 1 pcs

Protective fuse(2.5A,250V) 2 pcs

Protective tube(63mA,250V) 1 pc

Power Cord 1 pc
User manual Placed in a transparent 1 copy
Test report plastic bag 1 sheet
Quality certification card 1 sheet
Drain Valve 1 pc
Stainless steel hex cylinder head bolt 5 pcs
M10×20 steel flat washer10
Stainless 5 pcs
Stainless steel spring washer 10 5 pcs
Inner hexagon wrench 1 pc
Big rubber gasket 1 pc
Small rubber gasket 1 pc

3.3 Installation conditions and using environment

To avoid disturbances to the safety cabinet and its operator, follow the following guidelines, while
determining a suitable location for the cabinet:
Microbiological Safety Cabinet should be placed in airflow protected area. Front window operation
door shouldn’t be against over door or window,but far away from Ventilation and air conditioning
vents.In order to against outside various high-speed current of air to interference of operation area,
such as door, window, fan, draught of sending air, compressed air etc.
Test shows that if other interference airflow exceeds the intake airflow rate of the safety ca
binet, the indoor air will enter the working area of the cabinet.So it is very necessary to place the cabi
net in the correct position.It should also pay attention to the relationship between the safety cabinet e
xhaust air and indoor ventilation airflow or exhaust duct.Exhaust air discharged from the top , When

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placing the cabinet, it should avoid restricting its exhaust.Biological safety cabinets should be in the
direction of the air flow downstream and the side of the security cabinet at least 300mm space to help
check.
Working environment:
(1) Only is suitable for indoor use;
(2) Altitude not exceeding 2000m;
(3) Ambient temperature: 15℃-35℃;
(4) Relative Humidity: ≤75%;
(5) Atmospheric pressure range: 70 kPa-106 kPa;
(6) Electrical parameters: Consistent with the rated voltage of the Microbiological Safety Cabinet
(See 2.1.5 technical parameter performance index);
(7) Power supply need to be grounded; (Judging method: testing the Jine phase and the neutral phase
of the power supply with multimeter, the Jine phase to ground voltage should be grid voltage and
the neutral phase to ground voltage should be 0, otherwise the power supply ground is bad).
(8) Overvoltage category: II
(9) Pollution degree: 2

3.4 Installation
a. Remove all the package materials;
b. Inspect the surface of main body to make sure whether there is scratch, deformation or
uncorrelated things;
c. Chor forklift;eck the attachment and information according to the packaging list in instruction.
d. Move the whole device to the final installation location,where is convenient to install.
e. This equipment shouldn’t be hosting.Wooden packaging should be used as tap support in transpor
process, carried by hydraulic truck or forklift.
f. The whole power line 3 * 1.0mm² * 3000mm; the whole ground line is yellow-green Φ1.0mm2

The base stand will be packed at back of main body, please take it out before installation.
DO NOT INVERT, DISASSEMBLE OR TITILE THE CABINET during transportation.
g. The base stand assembly
Referring to Picture 4 assemble the base stand.

Note: The base mounting bolts are fixed to the base stand. When install the base stand,
except the 4 bolts marked with red circle in Picture 5, please remove the other bolts and install.

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 Right bracket

The bolts in the red ring

shall not be removed

M10 * 20 hexagon socket

Left bracket
head cap screw

Picture 4

Remove the M10×20 Hexagon socket head screws, Buckle plug, M10 Stainless steel flat washer,
M10 Stainless steel spring washer, M10 Stainless steel cap nuts, referring to Picture 5 assembled
base, firmly fastening requirements.
For the connection between the cabinet and the base, it is recommended to use an elevator.
Refer to Picture 5 to connect base stand and main body

Cabinet mounting
holes

Locating bolts

Picture 5

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Take out the M10*20 Hexagon socket head screws, Flat washer 10, Spring washer 10 from the
accessory box, and fasten tightly according to Picture 6.

M10*20 Hexagon socket

head bolt + flat washer 10 +
spring washer 10 ,through the
base, connect with the M10
welded nut on the bottom
of the cabinet side
Picture 6

h. Adjustment of Foot master Caster

Picture 7

Clockwise rotate caster’ red part to low down the base feet and the height of the cabinet. Low down
all four casters can move the cabinet position. Counterclockwise rotate casters’ red part can rise the
base leg and height of cabinet. Adjust all four casters at the same time can makes cabinet stable.
i. Installation of Drain valve.

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 Picture8

1. Drain valve connecter

2. Shim (Inner diameter*outer diameter*thicknessΦ20*Φ28*2mm)
3. Safety cabinet bottom installation holes
4. Ball coupling fastening nut
5. Rubber gasket (Inner diameter*outer diameter*thicknessΦ13*Φ19*2mm)
6. Drain valve
Take out drain valve coupling, shim(Inner diameter*outer diameter*thicknessΦ20*Φ28*2mm), Ball
nut, Rubber gasket(Inner diameter*outer diameter*thicknessΦ13*Φ19*2mm), Drain valve,
assembling from up to down as Picture 8 illustrated.

Don’t place equipment where difficult to operate and disconnect device.

3.5 Checking after installation

Checking Items Normal situation

Inflow （0.53±0.025）m/s ,downflow

Wind speed display
（0.33±0.025）m/s

Blower Blower runs normal

Fluorescent lamp Lamp lights after pressing button

Lamp lights after pressing button (please

UV Lamp
close the glass door first)

Display screen buttons All buttons can be used

Press the socket key, multimeter testing

Socket
output supply voltage

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 4. User Instructions
4.1 Functions
4.1.1 Product Concept
This cabinet defined as class II A2 type Microbiological Safety Cabinet, It totally meets the
European standard for biological cabinet EN 12469:2000 standard. Microbiological Safety Cabinet is
a kind of negative pressure filtration system for protecting operator, the laboratory environment and
work materials, the front opening which air flow inward have protection function for operator, the
filtered laminar flow generated by vertical ULPA can protect work materials, what’s more, the
polluted air flow become pure after processed by ULPA filter. When it’s used in microbiology
experiment environment filled with volatile or toxic chemical and radionuclide, suitable exhaust
hood in function have to be linked.
4.1.2 Application Range
Microbiological Safety Cabinet is necessary equipment in the laboratory in the search of
microbiology, biomedical, DNA recombinant, animal experiment, and biological products, especially
in the occasion that operator need to adopt protective measure, such as medical and health, pharmacy,
medical research. Our equipment provides a safety working environment which don’t have bacterial
and dust in the process of bacterial culture.
4.1.3 Working theory/Air flow pattern and protected area

Picture9
4.1.4 Protected objects
Microbiological Safety Cabinets (BSCs) are designed to protect the operator, the laboratory
environment and work materials from exposure to infectious aerosols and splashes that may be
generated when manipulating materials containing infectious agents, such as primary cultures, stocks
and diagnostic specimens.

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4.1.5 Technical parameters
Model
BSC-4FA2-HA
Parameters

Power Supply （230V±10%）；50Hz

External
1383*800*2260（mm）
Size(W*D*H)

Working Zone
1280*600*650（mm）
Size(W*D*H)

Rated power 1200 W

Total Airflow
490 m3/h
Volume

Downflow Velocity 0.33 m/s

Inflow Velocity 0.53 m/s

Fluorescent lamp
14W*2
Consumption

UV Lamp
30W
Consumption

ULPA Filter 99.9995％（Diameter:0.12μm）

Noise ≤65dB(A)

Socket 230V * 3.0A(1)

Notes :(1) Electric consumption power including two powers which operation area needs to load,the
total load power is 230V * 3.0A.
(2) The Company reserves the right to change the design of product, we will change design of
product without prior notice.
4.1.6 Performance Index
1) Biological safety functions
Personnel protection, tested by potassium iodide (KI) method, the protection factor of the front
window operation port should not be less than 1×105;
Sample protection, microbial colony count ≤5CFU;
Cross contamination protection, microbial colony count ≤2CFU.
2) Leak-proof Cabinet
If cabinet pressurized to 250Pa, Paint 25 g/L of soap solution with a paint brush, without any leakage
of soap bubbles.
3) Integrity of ULPA Filter

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Scan and detect the ULPA filter, the leakage rate at any point should not be ＞0.01%.
4) Vibration amplitude
The net vibration amplitude between frequency 20Hz and 2000Hz is no more than 5μm (rms).
5) Illumination
The work surface illumination is no less than 750 lux.
6) Electrical properties
Basic insulation: between live parts and the metal shell in the AC voltage 1500V, continuous 1min
without breakdown.
Reinforced insulation: between live parts and non-metallic shell in the AC voltage 3000V, continuous
1min without breakdown.
Leakage current ≤10mA.
Grounding resistance ≤0.1Ω

4.2 Product structure

4.2.1 Structural composition of BSC-4FA2-HA

1 Fuma caster
2 Alarm rest plate
3 Air intake grid
4 Door glass
5 LCD display
6 Power lock
7 Fluorescent lamp
8 UV lamp
Side window
9
glass
10 Waterproof socket
11 Stepper motor
Counterweight
12
plate
Picture10

4.2.2 Structure introduction

1) Driving System of Front Window
The glass door drive system consists of stepping motor(Optional), front glass door, traction
mechanism, counterweight, limit switch and so on.
2) Air Filtration System
Air Filtration System is the most important system of BSC. It consists of blower, supply filter and
exhaust filter. The function of Air Filtration System is transferring filtered air to work area, ensure

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the down flow velocity, and keep Class 10 cleanness of work area.
3) UV Light
UV lamp is inside work area. So UV lamp can well sterilize all space of work area. Emission of
253.7 nanometers can ensure most efficient decontamination.
4) LED lamps light source
LED lamps are used for lighting to ensure that the average illumination in the operation area meets
the standard requirements.
5) Sampling port
The sampling port reserved on the safety cabinet for testing the aerosol concentration of the upstream
of the ULPA filter is located at the lower part of the worktable in the working area. The transparent
pipes marked with"filter upstream" are the sampling ports for testing the aerosol concentration of the
upstream of the air supply filter and air exhaust filter, as shown in Picture11.

Picture11

The air pipe should not be blocked and please do not hang anything on the pipes,
otherwise it will affect wind speed and pressure.
6) Waterproof Socket
Two waterproof socket are arranged at the back of the operation area, which can supply power to the
equipment in the operation area. The user can power on/off waterproof socket through the socket
key.

Please make sure the total load of sockets should be ≤ 230V*3.0A；

The equipment is equipped with the main power fuse and waterproof socket fuse, which are located
on the strong electrical circuit board. Each fuse has its one label, where the user can see the model
and specification of the fuse.
7) Power lock
When the power cord is connected to main power, switch the key for power lock, then the equipment
is powered on.
8) Capacitive touch screen

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Capacitive touch screen works by the current induction of human body. Capacitive touch screen is a
four-layer composite glass screen. The inner surface and interlayer of the glass screen are coated
with a layer of ITO, respectively, and the outer layer is a thin layer of silica glass protective layer.
The interlayer ITO coating is used as the working face, four electrodes are led out from four corners,
and the inner layer ITO is the shielding layer to ensure a good working environment. When the
finger touches the metal layer, due to the electric field of the human body, a coupling capacitance is
formed between the user and the surface of the touch screen. For high-frequency current, the
capacitance is a direct conductor, so the finger absorbs a small current from the contact point. The
current flows out from the electrodes on the four corners of the touch screen, and the current flowing
through the four electrodes is proportional to the distance between the finger and the four corners.
The controller obtains the position of the touch point through the accurate calculation of the four
current ratios.

Picture 12

The touch screen display technology adopted by our company can reflect the working condition of
the instrument and equipment in real time, such as the effective working state of the filter.
9) Structure
a) There are side windows on both sides of the biological safety cabinet to facilitate observation
of the experiment.
b) The glass door can be used manually and electrically at the same time, and the glass door can
be pulled down to close the front window when the power is off.
c) Microbiological Safety Cabinet’s both sides and back area are negative pressure air channel.
And the negative pressure keeps work area away from contamination.
d) Cabinet body is built of 1.2mm cold-rolled steel with anti-powder coating. Strong and steady.
e) Work area is fully made of 304 stainless steel which looks beautiful and with corrosion
resistance performance.
f) Base stand is made of cold-rolled steel with anti-powder coating.
g) Soft touch type control panel, easy to handle and beautiful appearance.

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10) Warnings and reminders
Digital differential pressure display, digital wind speed display, audible and visual alarm system.
a）Alarm for glass door exceeding safety height
When the front glass door is too high, it will give an audible and visual alarm, and the alarm will
flash on the touch screen. At this time, you only need to adjust the height of the front window glass
(the opening height of the glass door is set at 200 mm).
b）Filter differential pressure alarm
If the exhaust and supply air filter of the equipment fails to meet the specified requirements, it will
give an audible and visual alarm, and the alarm will flash on the touch screen to prompt the user to
replace the filter immediately, so as to protect the safety of the user.

4.3 Control panel

Picture 13
1 Fan 2 UV 3 Lighting 4 Settings
5 Socket 6 Silence 7 Screen-locking 8 Glass door up/ down
Exhaust filter
Alarm display Clock Dynamic diagram
9 10 11 12 differential pressure
window display of airflow pattern
display of
Air supply filter Inflow wind
Down-flow wind
13 differential 14 speed 15
speed display
pressure display display

4.3.1 Touch screen display window

Through the touch screen display window, you can view the working status and performance of the
device.

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4.3.2 Buttons
Password interface: after power on, the password interface will pop up. The initial password is 2222,
and the password can be modified. The modification method is shown below.
Button function: After pressing the corresponding function button, there will be icon display on the
display screen and the corresponding function will be started.
In case the fan button is pressed, there will be an animation demonstration after the fan runs; and the
fan will stop automatically when the glass door is closed.
Press the UV button to turn on the UV disinfection lamp and press it again to turn off the UV
disinfection lamp.
When the lighting button is pressed, the LED lamp is turned on, and when pressed again, the LED
lamp is turned off.
When the socket button is pressed, the socket is powered on, and if pressed again, the socket is
powered off.
Horn button: when the machine is in alarm state, press this button to cancel the alarm, and press it
again to start the alarm function.
Lock screen function: touch the lock screen button, the operation interface is in the locked state, at
this time other function buttons are in the locking state to prevent disoperation.
Interlock: UV lamp and fan are interlocked with each other. That’s to say, UV lamp cannot be turned
on when the lighting button is turned on; when UV lamp is turned on, the LED lamp can be turned
on and UV lamp is turned off; Note: when the fan is in normal operation, if the glass door is closed,
the fan will stop working, but there is an icon. After the glass door is lifted, the fan will resume
operation, and the UV function is the same as above.
Alarm window: when an alarm is triggered, the alarm information will be displayed in the
corresponding alarm window. When multiple alarms appear at the same time, it will be broadcast
circularly.
Button memory function: fan, LED lamp and lighting are provided with power-off memory function.
If the power is turned on after power failure, the corresponding function button will be opened,
where there is no need to re-input the password but enter the main interface.
User setting button: setting interface (click setting button in the main interface to enter the settings
interface).
Date and time adjustment: click the time in the upper right corner of this interface to enter the time
adjustment interface. To adjust the corresponding date and time in this interface, press the OK button
to save and exit.
Timing on / off function: click Time Mode button in the user settings interface to enter the setting
interface. Under this interface, the timing on/off function and timing shutdown function of fan, UV
lamp and socket can be set. For specific operation, input the time for timing on/off operation in the
corresponding function bar, and press the corresponding ON button after input to turn on the function.
At the same time, in the main interface, there will be an icon prompt in front of the corresponding
operation button, and the corresponding function will automatically be on/off according to the set
time. To turn off this function, click OFF button. Note: fan and UV lamp functions are interlocked by
glass doors.
View the working time of filter, UV lamp: click the Device Life button in the user settings interface
to enter the view interface to view the service life.
User password modification: in the settings interface, click the Change Password button to enter the

21
password modification interface. Enter the original password to enter the modification interface to
modify the user password and click “confirm” button to exit.
Glass door up button: press the up button continuously, the glass door will continue to rise, and stop
when it is 200 mm away from the worktable, continue to press the up button until the lowest point of
travel is reached, and release the button, the glass door will stop moving.

4.4. Instructions for Operation

a、Connect the same power reply, as required of equipment
b、Open the power lock, LCD display lights up and alarm rings at the same time, then the machine
enters to standby status. Wait for the operator to input button to use it.
c、Press POWER button, then the following functions are available: Fluorescent lamp, UV lamp,Fan,
Mute, Sockets, Front window up and down, Reservation timing

When front window is closed and fluorescent light is off, then press the UV button to
select the sterilization function.

When UV lamp working avoid looking straight.

d、Please move the front window at 200mm height from the worktable, turn on the fan, make sure the
experiment should be started after fan working for at least 10 minutes.

(1) For operating safety, please put testing materials inside the cabinet in advance, and
keep the front window at 200mm height from the worktable during operation.
(2)UV lamp intensity should be tested regularly. If there is no test conditions, it should be
replace when the UV timer on the display indicate the working time reaches to 1000 hours.
e、Please move the front window at 200mm height from the worktable, turn on the fan, make sure the
experiment should be started after fan working for at least half an hour.

For operating safety, please put testing materials inside the cabinet in advance, and keep
the front window at 200mm height from the worktable during operation.
After finishing the experiment, please move the front window down to the bottom, and make sure to
sterilize the cabinet by UV lamp for 30 minutes before turning off the cabinet.

4.5 Daily maintenance

Because the operating time will directly affect the judgment of maintenance needs, we recommend
the user keep a detailed record of operating time for reference.

When doing maintenance, please pay attention to cut off the power, so as to avoid electric
shock!
4.5.1 Preparations before maintenance
Soap, hot water or warm water, a soft cotton cloth, dry cloth or towel, medical alcohol or other
disinfectants, 100 dilution of household bleach, abrasive household cleaners, sterile water

Before fumigation should not open the cabinet enclosure, prohibit removing anyparts
from the Microbiological Safety Cabinet!

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4.5.2 Clean the cabinet surface
1) Clean the operating area surface
Wipe the entire surface with a soft cotton cloth or towel soaked with concentrated liquid soap, then
wipe up the soap with another cotton cloth or towel soaked with clean hot or warm water, and then
wipe the surface with a dry cotton cloth or towel rapidly.
Cleaning method: When the test substance is accidentally spilled on the work surface, firstly with a
paper towel to wipe up, then wash and clean with water or other materials (Notice: Cleaning material
used does not set off a chemical reaction with the test substance) , waste liquid discharges from the
bottom drain valve in the front left . After wiping twice, then use the solution of the test lytic
substance (Notice: The solution can used doesn’t set off a chemical reaction with the test substance)
to wash work surface, while using a hair brush cleaning twice, then wipe and wash with water or
other materials , waste liquid discharges from the sump drain valve in the left front, then clean the
sump.

Before fumigation should not open the cabinet enclosure, prohibit removing any parts
from the Microbiological Safety Cabinet!
Fumigation method: Safety cabinet should be sealed prior before fumigation to ensure that no
formaldehyde leak into the laboratory or another room. Fumigation for safety cabinet and filter
should ensure that export of ULPA filter and piping systems exposed for more than 14 hours in order
to ensure that microorganisms that pierce the filter is inactivate. When half of formaldehyde is
evaporated, turn on cabinet fan running for 10s - 15s, making the whole area filled with
formaldehyde; After complete evaporation, and then turn on the cabinet fan once more running for
10s - 15s. After the fumigation, Microbiological Safety Cabinet sealing device should be removed
and let the exhaust fan on for not less than 8 hours, so before using or servicing the cabinet to
remove residual formaldehyde vapor.
There are signs of contamination, work surface,sump and etc,we can use medical alcohol or other
disinfectant to wipe.
For the contaminated or dirty work surface or sump., use 70% medical alcohol or other disinfectant
to wipe.

Disinfectants used for wiping should not damage 304 stainless steel.
2) Clean the external surface and front window.
Use soft cotton cloth or towel to wipe the surface with non-abrasive household cleanser.
4.5.3 Overall maintenance period
We suggest comprehensive maintenance period is one year or 1000 working hours.
4.5.4 Maintenance methods
1) Daily and weekly maintenance
a. Disinfect and clean operating area;
b. Clean the external surface and front window around the operating area;
c. Check the various functions of equipment;
d. Record this maintenance result
2) Monthly maintenance
a. Clean the external surface and front window.
b. Wipe the working table, inner wall surface of operating area (excluding the wind distributing grid

23
 of operating area) and the inner surface of glass door with 70﹪ medical alcohol.
c. Check the various functions of equipment;
d. Record this maintenance result;
3) Annual maintenance
a. Check the two conveyor belts of front window drive unit and ensure that their tightness is
coincident.
b. Check the fluorescent lamps.
c. Apply for testing the overall performance of cabinet on an annual basis to ensure the
performance safety. User is responsible for testing costs.
d. Record this maintenance result.
4.5.5 Storage conditions
Safety cabinet should be stored in a relative humidity no more than 75%, the temperature is below
40℃, in the warehouse with good ventilation performance, no acid, no alkali and no other corrosive
gases, storage period shall not exceed one year, safety cabinet for more than a year needs to
unpacked and checked. Only the tested and qualified safety cabinet can be sold.

4.6 Methods and procedures for disinfection

Disinfection is necessary when any contaminated part of the Microbiological Safety Cabinet needed
for routine maintenance, replacement filters, and performance testing, etc. Before doing certification
test and gas sterilization, all internal working surface and the exposed outer surface should be
disinfected with a suitable disinfectant. Besides, before doing certificate test, please use the
biological safety level 2 designated agents, in the form of gaseous state to entire safety cabinet to
ensure the disinfection is in line with the requirements. When the safety cabinet has been used, it is
recommended to use the biological safety level 3 designated agents. It should be disinfected first
while the cabinet with the potential hazard of contamination move the place. In addition, if the
reagents are overflowed and spilled after the experiment, the contaminated surface should be
properly disinfected. Please use the depolymerization of paraformaldehyde as a disinfectant on the
occasion stated below, while most time use the gas. Before disinfection by other methods, the cycle
parameters and the validity of these parameters must be given for each model and size. The
compatibility of materials and the degradation and absorption of the alternatives are the key factors
to maintain the integrity of the safety cabinet and the time required for disinfection. There are some
cases need to use these alternative methods, e.g. slow virus disease. The disinfection methods should
be negotiated by the users and the authentication institution. When use paraformaldehyde for air
disinfection, it is necessary to point out the area of regulation, the gas masks, protective facilities, the
corresponding testing, medical monitoring, hazards training, record keeping and according to the
following steps:

Before disinfection, all hydrogen chloride must be removed from the safety cabinet.
Hydrogen chloride in the presence of formaldehyde in the air will form a carcinogen two
chloromethyl ether (BCME) with the environment condition.
a、Multiply the height, width and depth to calculate the total volume of the safety cabinet
b、Safety cabinet total volume multiplied by 11g/m3, to determine the required quality of
paraformaldehyde. To confirm the quantity of ammonium hydrogen carbonate or its substitute
according to the stoichiometric method and provide ammonia and formaldehyde with

24
 neutralization reaction. Ammonium hydrogen carbonate need to be more than 10% to ensure the
complete reaction.
c、If there is exhaust pipe in safety cabinet, the pipeline must be airtight. The terminal air tightness
in the pipe can be realized, or if there is a valve regulating valve closed in the safe cabinet near. If
the exhaust pipe is longer than 3m, please increase the amount of paraformaldehyde in order to
compensate for the increased volume. If safety cabinet exhaust gas recirculation into the building
exhaust system, please disconnect safety cabinets and building system connection and make it
closed (can use plastic film and plastic strip).
d、If the security cabinet is released exhaust into the indoor, please seal exhaust port with plastic
straps.
e、For emergency exhaust, sterilize and neutralize formaldehyde, please preplaced a hosepipe near
the safety cabinet. And this hosepipe should be connected with chemical through hood or other
suitable discharge of poisonous gas exhaust device.
f、 Place the heating device such as a commercially available electric heating frying pan, or
formaldehyde generator / neutralizer on the workbench and set the Temperature on 232℃ -246℃,
poly formaldehyde is evenly sprayed on the heating surface of the heating device.

The autoignition temp. of paraformaldehyde is 300℃.

g、Put the heating device used for neutralizer on the work surface. Neutralizer (ammonium
bicarbonate or equivalent substitute) it should be isolated from the air in the cabinet before we
use it . The following two examples tell us how to separate from air:
Example 1: Ammonium bicarbonate or substitute uniformly sprayed on the heating surface of the
heating device, cover it with aluminum foil to prevent ammonium bicarbonate or disinfection
alternatives reacting with formaldehyde. The place we put aluminum foil should enable the heating
ammonia escape, or to prepare removing the aluminum foil at the start in neutralization stage.
Removing the aluminum foil does not allow leaking out formaldehyde from Microbiological Safety
Cabinet;
Example 2: The safety cabinet with the glovesas a whole is sealed with plastic film. Ammonium
bicarbonate or equivalent substitute was placed in sealed containers in Microbiological Safety
Cabinet, in the neutralization stage personnel of disinfection through the glove disinfect the cabinet
without breaking the seal system. The ammonium bicarbonate or equivalent substitutes is removed
from the sealed container and uniformly sprayed on the heating surface of the heating device,the
heating device is energized,ammonium bicarbonate or equivalent substitute will be heated to release
ammonia;
h、Heating plate, water beaker and hygrometer should be put on the workbench for Microbiological
Safety Cabinet, do not connect the wires in the cabinet;
i、 Seal the front window working hole with a thick plastic film and plastic tape. Seal all possible
leak areas. Such as wire export,around front window working hole and the point where the
plastic film connect with Microbiological Safety Cabinet;
j、 Measured the temperature and humidity inside the cabinet;
k、Temperature should be above 21 ℃, humidity should be 60% to 85%. Heat water in the beaker
with heating plater to achieve the desired temperature and humidity;
l、 Before depolymerization formaldehyde in accordance with relevant provisions of the regulations
and strict security measures should limit access to the area around the cabinet or room.

25
 Occupational Safety and Health Administration guidelines on occupational exposure to
formaldehyde in claim airborne formaldehyde concentrations exceed permissible exposure limits
area should be prescribed as a control area, marked out with signs and symbols of the region,
only appropriately trained personnel. We must review and observe current regulations;
m、 Connect wire of the heating device with outside socket for Microbiological Safety Cabinet.
n、 After 25% of the formaldehyde is depolymerized, turn on the cabinet blower 10 s - l5 s.
Paraformaldehyde depolymerization 50%, repeat the above steps after the 75% and 100%. In
case the Microbiological Safety Cabinet blower does not work, use the auxiliary blower or fan to
promote air circulation inside the cabinet, or to extend the disinfection time, exceed the following
step p recommended time;
o、Disconnected the power of the heating plate and the heating device for paraformaldehyde ;
p、Microbiological Safety Cabinet keep for at least 6h, preferably overnight;
q、Prepare according to step g, power the heating device containing ammonium bicarbonate and
Microbiological Safety Cabinet to make ammonium bicarbonate vanish. Like operating with
paraformaldehyde , after 25% ammonium bicarbonate vanish,turn on the Microbiological Safety
Cabinet blower for 10 s - l5 s, if the fan does not work auxiliary fan or a fan to promote air
circulation in cabinet, or neutralization time extends to at least 6 h;
r、 Microbiological Safety Cabinet for at least 1 h after unfolding sealing film;
s、 The hose exhaust neutral formaldehyde out, tear plastic covers of biological safety cabinet vent ,
connect the hose with vent and seal, if the hose works, before the cabinet-through plastic window
cover will be inhaled, plastic cover ofthe front window working hole is cut out one or two small
openings (about 15 cm × 15 cm), make fresh air go into the cabinet, but neutral formaldehyde
from the Microbiological Safety Cabinet exhaust via hose.

You can use other methods to eliminate formaldehyde as long as the method safe and
effective to eliminate formaldehyde gas.

26
4.7 Replacement parts list
BSC-4FA2-HA Replacement parts list
No Designation Parameter

1 Lamp holder T8 GZ10

2 UV Lamp QT-FIT 5/8 1x18-39

3 LED Lamp PAK410110

4 UV lamp ballast QT-FIT 5/8 1x18-39

5 Upper filter (Exhaust filter) 800*430*69（mm）

6 Downflow filter (Supply filter) 1240*500*69（mm）

7 Fan DH230A2-AG5-05

LCD control board (strong circuit

board, weak circuit board, touch
8 Control panel
screen，Conversion board，Motor
board)

9 Front window 1314*700*6.0（mm）

10 Side window T（457/372.5）*688*5.0（mm）

27
4.8 Wiring diagram
BSC-4FA2-HA Wiring diagram

28
 5. Trouble shooting and Labels
5.1 Common faults & solution
5.1.1 Warning and reminder
Digital display of pressure difference, digital velocity display, audible and visual alarm system.
1) Over safety height alarm for front window
There will be audio and visual alarm when front window is lifting over safety height. Same time
LCD display will twinkle exclamation mark. Then just adjust the height of the front window.(Front
window height setting value is 200mm).
2) ULPA filter pressure difference alarm
There will be audio and visual alarm if pressure of air supply filter or exhaust filter can’t meet
present value, at the same time LCD display will twinkle exclamation mark. Remind the operator to
replace the filter immediately to protect the operator’s safety.
3) Velocity fluctuation alarm
There will be audio and visual alarm if the inflow velocity and down flow velocity below 20% of the
standard value, namely, inflow velocity below 0.42m/s, down flow velocity below 0.26m/s, at the
same time LCD display will twinkle exclamation mark to remind the operator pay attention.
5.1.2 Trouble shooting
Please confirm whether the power is connected or not, whether the power cord is obvious damaged
or not, whether the fuse is good or not, and whether the power locks are in the open state or not
before the fault diagnosis.
Faults Check parts Measures
Fluorescent lamp Tube Change it
doesn’t work Circuit Check circuit
Control panel Change it
UV lamp doesn’t First check the troubles according to the inspection of Fluorescent lamp, if
work the UV lamp still doesn’t work, then make the following analysis.

Micro Switch Check if Micro Switch is broken

Front window,
Check the front window, fluorescent lamp and the
Fluorescent lamp and
blower is open or not.
blower
Control panel Change it
Button doesn’t Make sure the power connects well and the fuse is
work well
Control panel Check if the button is broken
Make sure the connecting wire is connected well
Change control panel
Blower doesn’t Micro Switch Check if Micro Switch is broken or works fine
work
Front window Front window is open or not, blower works only
when the front window is open

29
 Blower If blower is broken, change it
Circuit Check circuit
Control panel Change it
No electricity in Socket Check if socket is broken
socket Socket fuse Check if socket fuse is broken
Circuit Check circuit
Control panel Change it
Pressure or air Gas circuit Check whether gas circuit has dropped, is broken,
speed displayed or jammed
incorrectly Control panel Change it
Front window Transmission part Check transmission connection and lead rail
doesn’t work
Circuit Check circuit
Control panel Change it
No electricity in Power supply Check power supply connects well
equipment
Power wire Check whether power wire has obvious damage
Fuse Check if the fuse is good
Power key Check if power key is open, is broken or not

Transformer Check whether the transformer works normally

Control panel Change it
Display doesn’t Connection winding Check whether the winding displacement
work displacement connects well.
Display screen Check whether the display screen is good

NOTES
(1) The above electrical parts must be operated by a qualified electrician in safety conditions
(cutting off power supply). The other parts are not allowed to remove; otherwise the user
should take responsibility by them;
(2) When failures are not occur and the operator can’t solve, please notify our maintenance
department immediately. For your safety, please do not maintain equipment by yourself;
(3) The maintenance of this equipment is undertaken by trained and recognized technicians;
(4) If you need to order parts, contact the agent or our technical service department, and please
indicate the model and serial number of the cabinet purchased.
5.1.3 Simple accessories replacement
1) Replace LED lamps light
When replacing, disconnect the power and open the operation panel in accordance with Figure 10.
Use the support frame of the operation panel (fixed on the inside of the operation panel as shown in
the figure), and then unplug the lead of the lamp in the direction shown by the arrow in Figure 11,
remove the amp, and install the corresponding type of lamp.

30
 Support bar

Take out one tip of

support bar behind
Hole plugs Remove the two hole plugs
the control panel to
from the left and right
mount on the cabinet
sides,Use screwdriver to
loose the screws

Picture 14
2)Replace the UV lamp
UV lamp should be replaced regularly according to the frequency of use, when using UV lamps
reach to the time of 1000 hours, we recommend to replace the lamp. When replacing, first make sure
the power is off, and then screw the bulb 90 °and take it off, then take the correspondence type of
lamp, and put it to the lamp holder and and screw 90 °in reverse direction. After replacing the UV

31
lamp, it needs to keep pressing the button of UV for about five seconds when the machine stays
standby.

Picture 15
3)Replace the fuse
Power plug fuse is located on the power supply board, when replace it, turn off the power and
disconnect plug, take out of the fuse and replace with a new fuse, and then press it back.

Picture 16

NOTE
(1) The above electrical parts must be operated by a qualified electrician in safety conditions
(cutting off power supply). The other parts are not allowed to remove; otherwise the user should
take responsibility by them;
(2) When failures are not occur and the operator can’t solve, please notify our maintenance
department immediately. For your safety, please do not maintain equipment by yourself;
(3) he maintenance of this equipment is undertaken by trained and recognized technicians;

32
5.2 Label Description

Biological hazard label

Ground label(Protected Ground

Earthing)

Safe operation glass door height

Filter scan label

Sewage valve label, sewage valve

biological hazard label, warning label

Waterproof socket power total capacity

Warning:Electrical Shock Attention

Use warning labels

33
 Operation area warning label

Operation area label

Label of air tap

Faucet label

Precautions for glass lifting

Front window warning film

UV lamp warning label

Electrical warning label

34
 Marking of left internal wiring

Marking of right internal wiring

Hole plugs

Support rod Remove label

Use instruction label for support rod

The switch warning label

35
 6.EMC
1) The device meets the emission and immunity requirements of EN 61326:2013.
2) The device is tested according to classA.
Biosafety cabinets need to take special precautions in terms of electromagnetic compatibility (EMC),
and must be installed and used according to the EMC information provided in this manual.
Perform-ance
Port Phenomenon Basic standard Test value
criterion

Enclosure Electrostatic discharge(ESD) IEC 61000-4-2 4kV contact discharge B

8kV air discharge B

Electromagnetic field IEC 61000-4-3 3 V/m (80 MHz to 1 GHz) A

3 V/m (1,4 MHz to 2 GHz) A

1 V/m (2,0 MHz to 2,7 GHz) A

Power frequency magnetic field IEC 61000-4-8 3 A/m (50Hz,60Hz)f A

AC power Voltage dip IEC 61000-4-11 0% during half cycle B

(including
0% during 1 cycle B
protective
earth) 70% during 25/30e cycles C

Short interruptions IEC 61000-4-11 0% during 250/300e cycles C

Burst IEC 61000-4-4 1kV (5/50 ns, 5kHz) B

Surge IEC 61000-4-5 0,5kVa)/1kVb) B

Conducted RF IEC 61000-4-6 3V (150kHz to 80MHz) A

Portable and mobile RF communication device can affect the operation of biosafety
cabinet.

In addition to the converters and cables allowed by the manufacturer of the biosafety
cabinet as replacement parts for internal components, the use of accessories, converters and
cables that are not specified may increase the radiation or reduce the anti-interference
capability of the biosafety cabinet

The biosafety cabinet should not be used close to or stacked with other device. If it must
be used close to or stacked with other device, it should be observed and verified if it can
operate normally under the configuration.

36
BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: export@biobase.com
Complaints: customer_support@biobase.cc
After-sales service: service_sd@biobase.cc; service_ivd@biobase.cc
Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com