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Gram Staining MCQs ARS

The document contains multiple-choice questions (MCQs) focused on Gram staining techniques for the ARS Prelims Exam. Key concepts include the development of the technique, the roles of various stains and reagents, and the differences between Gram-positive and Gram-negative bacteria. It also covers the implications of improper staining procedures and alternative techniques for specific bacteria.

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349 views2 pages

Gram Staining MCQs ARS

The document contains multiple-choice questions (MCQs) focused on Gram staining techniques for the ARS Prelims Exam. Key concepts include the development of the technique, the roles of various stains and reagents, and the differences between Gram-positive and Gram-negative bacteria. It also covers the implications of improper staining procedures and alternative techniques for specific bacteria.

Uploaded by

dr.rangot
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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MCQs on Gram Staining Techniques for ARS Prelims Exam

1. Who developed the Gram staining technique?


(a) Hans Christian Gram

2. Gram staining is used to differentiate bacteria based on their:


(c) Cell wall composition

3. The primary stain used in Gram staining is:


(b) Crystal violet

4. The mordant in Gram staining is:


(a) Iodine

5. The decolorizing agent used in Gram staining is:


(d) Alcohol or acetone

6. The counterstain in Gram staining is:


(c) Safranin

7. Gram-positive bacteria appear:


(b) Purple or blue

8. Gram-negative bacteria appear:


(d) Pink or red

9. The primary function of iodine in Gram staining is:


(c) To form a complex with crystal violet

10. The role of alcohol in Gram staining is to:


(a) Decolorize Gram-negative bacteria

11. Gram-positive bacteria retain the crystal violet stain because of:
(c) Thick peptidoglycan layer

12. The lipid content in the cell wall of Gram-negative bacteria is:
(b) Higher than Gram-positive bacteria

13. The peptidoglycan layer in Gram-negative bacteria is:


(d) Thin

14. Why do Gram-negative bacteria lose the primary stain?


(a) Alcohol dissolves the outer membrane

15. The final step in Gram staining is:


(c) Counterstaining with safranin

16. If iodine is skipped in Gram staining, what will happen?


(b) Gram-positive bacteria may appear pink

17. If alcohol is overused in Gram staining, what will happen?


(c) Gram-positive bacteria may also get decolorized

18. The Gram reaction depends on:


(a) Cell wall composition

19. What happens if decolorization is too short?


(b) Gram-negative bacteria may appear Gram-positive

20. The outer membrane of Gram-negative bacteria contains:


(c) Lipopolysaccharides (LPS)

21. Gram staining is classified as a:


(b) Differential staining technique

22. Gram staining is most useful for:


(a) Bacterial classification

23. The alternative counterstain to safranin is:


(c) Carbol fuchsin

24. Which staining technique is used for Mycobacterium instead of Gram staining?
(d) Acid-fast staining

25. Gram-variable bacteria show:


(b) Mixed pink and purple cells

... (truncated for brevity, the full list will be included in the PDF)

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