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Microscope Lab Brian

The document outlines the procedure for using a compound light microscope, detailing its parts and their functions. It describes an experiment to test the hypothesis that higher magnification results in a larger image, which was confirmed through observations of the letter 'e' and an insect sample. The lab was deemed successful as the hypothesis was proven correct, and additional observations were made about the specimens.

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8 views6 pages

Microscope Lab Brian

The document outlines the procedure for using a compound light microscope, detailing its parts and their functions. It describes an experiment to test the hypothesis that higher magnification results in a larger image, which was confirmed through observations of the letter 'e' and an insect sample. The lab was deemed successful as the hypothesis was proven correct, and additional observations were made about the specimens.

Uploaded by

252345
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Brian Nakatani

Microscope Lab
Carrico
B1
8/28/2024

How do you use a compound light microscope?


1. base: The base of the microscope is the part that holds the microscope and touches the
floor
2. Stage: The stage of the microscope is where you put your specimen for examination.
3. Illuminator: The illuminator lights up the specimen so that you can better see t
4. Coarse focus: The big coarse focus knob moves the stage up and down in big
movements
5. Fine focus: The fine focus knob moves the stage up and down in smaller but more
precise increments than the coarse focus
6. Arm: The arm of the microscope holds the lens and the eyepiece above the stage
7. Lens: The lens is what actually magnifies the specimen and can be rotated to have a
higher or lower magnification
8. Eyepiece: The eyepiece is what you look through to see the magnified specimen.
Hypothesis
If I use a higher magnification lens then the specimen will appear larger because a higher
magnification is related to a bigger image.

Materials:
1. 1x compound light microscope
2. 1x 4x zoom lens
3. 1x 10x zoom lens
4. 1x 40x magnification lens
5. 1x slide
6. 1x cover slip
7. 1x letter “e” from newspaper
8. 1x drop of water
9. 1x sheet of paper
10. 1x small circular object
11. pencil/colored pencils
12. One insect prepared sample
Procedier
1. Plug in the compound light microscope and turn it on
2. Put the letter “e” on the microscope slide, put a drop of water on it and carefully put a
cover slip on it and then load the prepared slide onto the microscope stage
3. Center the “e” on the center of the stage
4. Set the zoom on the microscope to 4x zoom
5. Look in the eyepiece and use the coarse adjustment knob to focus the microscope until
the “e” is clear
6. Use the circle object to trace a perfect circle on your paper and then draw what you see
in the eyepiece
7. Adjust the lens to the 10x lens, repeat steps 5-6 except that you might want to use the
fine adjustment knob to focus
8. Adjust the lens to the 40x lens, repeat steps 5-6 except that you might want to entirely
use the fine focus knob
9. Lower the stage and replace the “e” slide with the insect slide
10. Set the lens to 10x, adjust the focus until the insect is clear, then Use the circle object to
trace a perfect circle on your paper and then draw what you see in the eyepiece
The purpose of this Lab was to investigate the purpose of a microscope,
My data shows that when the microscopes lens were changed to higher magnification the image
got bigger. This was shown with the attached data and observations, the 40x image show the
“e” flipped horizontally and vertically. The 100x image shows that the newspaper was made out
of fibers and those fibers are impossible to see with the naked eye. The 400x image shows that
the “e” itself is colored black using drops of ink.

This data proves my hypothesis that If I use a higher magnification lens then the specimen will
appear larger because a higher magnification is related to a bigger image, because when I used
a higher magnification lens, the image did indeed get bigger. This strongly supports my
hypothesis because the zoom for the lens was changed in correlation with the number on the
lens.

During this lab I might have made a mistake when putting the coverslip on the “e” because there
were still bubbles on the view. This may have skewed the results because the distortion from
the bubble may have changed what I would have seen. This lab was also useful because I
learned how to use a microscope and I made a second observation during this lab. The
observation was that the newspaper of which the “e” was printed on is made of strips of paper
that are somehow bound together..

In conclusion this lab was a complete success, because my hypothesis was proven correct, I
learned how to use a microscope, and I made a second observation.

1. Why do you place one hand under the base of the microscope as you carry it?
To avoid risk of dropping it
2. What Kind of light source does your microscope have?
An illuminator on the base
3. How is the image of an object seen through a high power objective different from the
image seen through a low power objective?
The image is bigger and more magnified
4. How does the letter “e” as seen through a microscope differ from the way “e” normally
appears.
It is upside down and backwards
5. When you move the slide to the left what direction does it appear to move?
Right
6. When you move the slide up, what direction does it appear to move?
Down

1. Explain why a specimen to be viewed under a microscope must be thin.


Because if it is not the thickness will interfere with the lens and be hard to view.
2. Why should a microscope slide and coverslip be held by their edges
So you don't get them dirty
3. Why should you use a piece of lens paper only once
Because after the first time you might have specimens mix and that is bad.
4. Why is it a good idea to place your microscope away from the edge of your table.
So it doesn’t get knocked off and break
5. Suppose you were observing an organism through a microscope and it moved down and
then to the right, what does this tell you about the actual movement of the organism?
It moved up and then to the left

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