Patient Name : Ms.

SOUNDARYA AELIGALA Collected : 27/May/2025 06:09PM

Age/Gender : 31 Y 4 M 30 D /F Received : 28/May/2025 09:17AM
UHID/MR No : APJ1.0015528877 Reported : 29/May/2025 10:28AM
Visit ID : DMKNOPV46903 Status : Final Report
Ref Doctor : Dr.SELF Client Name : APOLLO 24X7
IP/OP NO : Center location : Lanco Hills,Hyderabad

DEPARTMENT OF MOLECULAR BIOLOGY.

COVID 19 RT PCR WITH HOME COLLECTION

Test Name Result Unit Bio. Ref. Interval Method

COVID-19(SARS-CoV 2)-REAL TIME PCR(RT-PCR)
SAMPLE TYPE PHARYNGEAL SWAB
SARS-CoV-2 DETECTED
N-Gene CT VALUE 22
ORF1ab Gene CT VALUE 24

Comment:
Biological Reference Rang
*Ct value < 35 is Positive
*Ct value >35 is Negative

*Ct cutoff suggested by ICMR

Ct value does not correlate with
disease severity

Please Note:
*CT values vary with the type of kit used, the types of samples collected and the various pre-analytical factors.
*The published studies are conflicting to definitively prove a direct correlation between disease severity/infectiousness and CT
values therefore; relying on CT values for determining infectiousness of COVID-19 patients and deciding management protocols
is left to clinical discretion.

Kit used: CoviPath ™ COVID-19 RTPCR KIT

Result Interpretation
Indicates presence of detectable levels
Detected (Positive) Result
SARS-CoV-2 specific RNA (ORF1ab gene and/ or N gene) in patient’s sample.

Indicates absence of detectable levels

Not Detected (Negative) Result
SARS-CoV-2 specific RNA (ORF1ab gene and/ or N gene) in patient’s sample.

Page 1 of 4

SIN No:MO02990071
This test has been performed at Apollo Health and Lifestyle Ltd- Hyderabad.
Patient Name : Ms.SOUNDARYA AELIGALA Collected : 27/May/2025 06:09PM
Age/Gender : 31 Y 4 M 30 D /F Received : 28/May/2025 09:17AM
UHID/MR No : APJ1.0015528877 Reported : 29/May/2025 10:28AM
Visit ID : DMKNOPV46903 Status : Final Report
Ref Doctor : Dr.SELF Client Name : APOLLO 24X7
IP/OP NO : Center location : Lanco Hills,Hyderabad

DEPARTMENT OF MOLECULAR BIOLOGY.

COVID 19 RT PCR WITH HOME COLLECTION

Indicates the target concentration is too low, or presence of interfering substances

Indeterminate Result
leading to PCR inhibition.

NOTE:

If either of the genes cannot be determined or will give indeterminate results, further testing maybe required using another kit
with a different primer and probe.
A Not Detected (Negative) Resultdo not preclude SARS-CoV-2 and should not be used as the sole basis for patient
management decisions. Kindly repeat test after 48 to 72 hrs if clinically suspected.
Lower respiratory tract specimens are more representative and are preferred.
If at a later date, suspicion of COVID-19 is strong, a fresh sample for Real Time PCR after a gap of 2-4 days after collection
of first sample maybe considered.
Please contact your Physician for necessary action to be taken and to limit the spread of infection.
Clinical Correlation and Correlation with the history of the patient is required before arriving at any conclusion. Presence of
Non-Specific interfering substances during this assay to be kept in mind. Please correlate clinically before arriving at any
conclusion.
False positive and false negative results can be due to multiple factors including sampling technique, transport & interference in
the assay can affect the result.
Presence PCR inhibitors in sample may lead to false negative or invalid results.
Mutation in the target sequence of SARS-CoV-2 or change in the sequence due to virus evolution may lead to false negative
results.
Invalid Result: There is no typical S-shape amplification curve or Ct >35 or No Ct detected for target genes and internal
control, indicating the specimen concentration is too low, or there are interfering substances that inhibit the reaction. If upon
retest, the result is invalid again, another fresh sample should be collected and tested.

SARS-CoV-2 Virus:

SARS-CoV-2 is an enveloped single stranded positive sense RNA virus belongs to family Coronoviridae. It carries largest single
stranded RNA genome of ~30kb and is the causative pathogen of ongoing COVID-19 pandemic (3). The capsid consists of the
nucleocapsid protein N and this is further surrounded by a membrane, that contains three proteins: the membrane protein (M) and the
envelope protein (E), which are involved in the virus budding process, and the spike glycoprotein (S), which is a key player in binding
host receptor and mediating membrane fusion and virus entry into host cells (4).
Page 2 of 4

SIN No:MO02990071
This test has been performed at Apollo Health and Lifestyle Ltd- Hyderabad.
Patient Name : Ms.SOUNDARYA AELIGALA Collected : 27/May/2025 06:09PM
Age/Gender : 31 Y 4 M 30 D /F Received : 28/May/2025 09:17AM
UHID/MR No : APJ1.0015528877 Reported : 29/May/2025 10:28AM
Visit ID : DMKNOPV46903 Status : Final Report
Ref Doctor : Dr.SELF Client Name : APOLLO 24X7
IP/OP NO : Center location : Lanco Hills,Hyderabad

DEPARTMENT OF MOLECULAR BIOLOGY.

COVID 19 RT PCR WITH HOME COLLECTION

Disclaimer:
This Test is based on real-time reverse transcriptase PCR technology for the qualitative detection of severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) specific RNA

*ICMR ID- DLUAHTMH-

*ICMR ID-AHLLBHT- HYDERABAD *ICMR ID-AHLVDLA- VIJAYAWADA
MUMBAI
*ICMR ID-ADNTKLKWB -
*ICMR ID-AHLLKDLKAP- KURNOOL *ICMR ID-AHLLDLDD-DELHI
KOLKATA
*ICMR ID-APHLILKDLCTN-
*ICMR ID-AHLLKDIALBK- BENGALURU *ICMR ID-AHHHSPPMH- PUNE
CHENNAI
*ICMR ID-APHLTHLFLADLCK-
LUCKNOW

(For kits determining RdRp gene or ORF1b, sensitivity / specificity of such kits shall be mentioned).

COVID-19 Advisory

Page 3 of 4

SIN No:MO02990071
This test has been performed at Apollo Health and Lifestyle Ltd- Hyderabad.
Patient Name : Ms.SOUNDARYA AELIGALA Collected : 27/May/2025 06:09PM
Age/Gender : 31 Y 4 M 30 D /F Received : 28/May/2025 09:17AM
UHID/MR No : APJ1.0015528877 Reported : 29/May/2025 10:28AM
Visit ID : DMKNOPV46903 Status : Final Report
Ref Doctor : Dr.SELF Client Name : APOLLO 24X7
IP/OP NO : Center location : Lanco Hills,Hyderabad

DEPARTMENT OF MOLECULAR BIOLOGY.

COVID 19 RT PCR WITH HOME COLLECTION

*** End Of Report ***

Page 4 of 4

SIN No:MO02990071
This test has been performed at Apollo Health and Lifestyle Ltd- Hyderabad.
 Patient Name : Ms.SOUNDARYA AELIGALA Collected : 27/May/2025 06:09PM
Age/Gender : 31 Y 4 M 30 D /F Received : 28/May/2025 09:17AM
UHID/MR No : APJ1.0015528877 Reported : 29/May/2025 10:28AM
Visit ID : DMKNOPV46903 Status : Final Report
Ref Doctor : Dr.SELF Client Name : APOLLO 24X7
IP/OP NO : Center location : Lanco Hills,Hyderabad

TERMS AND CONDITIONS GOVERNING THIS REPORT

1. Reported results are for information and interpretation of the referring doctor or such other medical professionals, who understand
reporting units, reference ranges and limitation of technologies. Laboratories not be responsible for any interpretation whatsoever
2. This is computer generated medical diagnostics report that has been validated by an Authorized Medical Practitioner/Doctor. The
report does not need physical signature.
3. Partial reproduction of this report is not valid and should not be resorted to draw any conclusion.
4. In the case you are not the intended recipient of this report. Please immediately return the same to the concerned issuing desk. Any
disclosure, copy or distribution of any contents of this report, is unlawful and is strictly prohibited.
5. Results delays may occur due to unforeseen circumstances such as non-availability of kits, equipment breakdown, natural calamities,
IT downtime, logistic delays or any other unavoidable event. For certain tests based on analyte stability, criticality of results and in the
interest of patient for having appropriate medical diagnosis, the same test may be outsourced to other accredited laboratory.
6. It is presumed that the tests performed are, on the specimen / sample being to the patient named or identified and the verifications of
particulars have been confirmed by the patient or his / her representative at the point of generation of said specimen
7. The reported results are restricted to the given specimen only. Results may vary from lab to lab and from time to time for the same
parameter for the same patient (within subject biological variation).
8. The patient details along with their results in certain cases like notifiable diseases and as per local regulatory requirements will be
communicated to the assigned regulatory bodies
9. The patient samples can be used as part of internal quality control, test verification, data analysis purposes within the testing scope of
the laboratory.
10. This report is not valid for medico legal purposes. It is performed to facilitate medical diagnosis only

SIN No:MO02990071
This test has been performed at Apollo Health and Lifestyle Ltd- Hyderabad.