Thermo Scientific

CW3 Cell Washer

Instruction Manual
50144259-f • 11 / 2016

Visit us online to register your warranty:

www.thermoscientific.com/labwarranty
 Table of Contents
1. Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1. 1. Items Supplied. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1. 2. Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1. 3. Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1. 4. Symbols Used on the Cell Washer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

1. 5. Symbols Used in the Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2. Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2. 1. Directives and Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2. 2. Mains Supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2. 3. Location and Function of Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2. 4. Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2. 5. Condition-setting Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3. Transport and Set Up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3. 1. Before Setting Up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3. 2. Location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3. 3. Transporting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3. 4. Leveling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3. 5. Mains Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3. 6. Setting Up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3. 7. Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

3. 8. Shipping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4. 1. Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4. 1. 1. Injection Volume Adjustment and Pump Ventilation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4. 1. 2. Setting the Operating Conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4. 1. 3. Preparation of the Sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4. 2. Operation Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Thermo Scientific CW3 | 3



4. 2. 1. Automatic Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4. 2. 2. Manual Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4. 2. 3. Agitation-Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4. 2. 4. Operational Sequence for Antiglobulin Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5. Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5. 1. Pump, Tank and Tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5. 2. Rotor, Distributor and Bowl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5. 3. Chamber, Splash Guard, Drain Cover and Door Stopper Parts. . . . . . . . . . . . . . . . . . . . . . . 36

5. 3. 1. Drain Cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5. 3. 2. Splash Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5. 3. 3. Checking and Replacing the Door Stopper Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5. 4. Shipping and Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

6. Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

6. 1. Mechanical Emergency Door Release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

6. 2. Error Codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

6. 3. Troublshooting when no Error Code is indicated. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Decontamination Information Certificate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Chemical Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

4 | CW3 Thermo Scientific

 Items Supplied

1. Preface

1. 1. Items Supplied
Article No. Description Quantity
Thermo Scientific CW3 cell washer 1

S402776A Bowl Assembly 1

S413259C Tank (5 L) 1

4744346 Tubing 3m

75000015 Tube Connector 1

S4011034 Drain Tube 2.5 m

Test Tubes (12 mm in diameter and 75

480270 50 (1 box)
mm in length)

76003500 Grease 1

8046004 Hex. Wrench 1

S411107 Motor Guard Plate 1

Hose Band
4666354 Small 1
4666357 Medium 1
4666355 Big 2

S413181A L-shaped Adapter 1

480879 D10 Adapter 25

Power Cord 1

Instruction Manual 1 

Rotor Assembly Options 1

75000020 24 place rotor assembly with distributor

75000021 12 place rotor assembly with distributor

Thermo Scientific CW3 | 5

Items Supplied

Article No. Description Quantity

Replacement Parts

Rotor Assembly 1

75000022 24 places

75000023 12 places

Distributor Assembly 1

75000024 24 places

75000025 12 places

6 | CW3 Thermo Scientific

 Intended Use

1. 2. Intended Use
The Thermo Scientific CW3 cell washer is designed to perform cell washing in multiple washing cycles using saline solu-
tion. The cell washer provides blood cells after sample separation, which can be used for further blood testing such as
Antiglobulin test, ABO compatibility, Rh testing, Cross-matching and Antibody screening.
The centrifuge should always be operated by a trained individual such as a clinical laboratory technologist or a person
with a similar education.

1. 3. Precautions
Carefully read the following safety precautions for a thorough understanding.
Protection is impaired if the cell washer is not used as specified in the intended use.
Follow the instructions and procedures described in this instruction manual to operate this cell washer safely.
Be sure to observe the precautions in this instruction manual and on your equipment.
It is the general obligation of the operator to make sure, that the proper protective clothing is used. Mind the „Laboratory
Biosafety Manual“ of the World Health Organization (WHO) and the regulations in your country.
Safety messages are indicated as follows. They are stated with signal words combined with the safety alert symbol to call
your attention to items or operations that could be dangerous to you or other persons using this equipment. The definitions
of signal words are as follows:
ƒƒ WARNING: Warning notes indicate any condition or practice, which if not strictly observed, could result in seri-
ous injury or possible death.
ƒƒ CAUTION: Caution notes indicate any condition or practice, which if not strictly observed or stopped, could
result in personal injury, damage or destruction of the equipment.
ƒƒ NOTICE: Notes indicate an area or subject of special merit, emphasizing either the product’s capabilities or
common errors in operation or maintenance.
Do not operate this cell washer in any manner not described in this Instruction Manual. Should you have any troubles with
this cell washer, call a Thermo Fisher Scientific authorized sales/service representative.
The precautions described in this Instruction Manual are carefully developed to cover all possible risks. It is important that
you are alert for unexpected incidents. Be careful when operating this cell washer.

Thermo Scientific CW3 | 7

Precautions

WA R N ING

1. The cell washer is neither inert nor protected against explosion. Never use the cell washer in an explosion-prone
environment.
2. Do not set up the cell washer in or near places where flammable gases are generated or chemicals are stored.
3. If centrifuging any hazardous materials mind the „Laboratory Biosafety Manual“ of the World Health Organization
(WHO). Look on the internet page of the World Health Organization (www.who.int) for the „Laboratory Biosafety
Manual“.
For materials in a higher risk group, extra safety measures have to be taken.
4. Take all necessary safety measures before using samples that are toxic or radioactive, or blood samples that are
pathogenic or infectious. You use such samples at your own responsibility.
a. If the cell washer, rotor, or an accessory is contaminated by samples that are toxic or radioactive, or blood
samples that are pathogenic or infectious, be sure to decontaminate the item according to good laboratory
procedures and methods.
b. If there is a possibility that the cell washer, rotor, or an accessory is contaminated by samples that might
impair human health (for example, samples that are toxic or radioactive, or blood samples that are
pathogenic or infectious), it is your responsibility to sterilize or decontaminate the cell washer, rotor, or
the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales/service
representative.
c. It is your responsibility to sterilize and/or decontaminate the cell washer, rotor, or parts properly before
returning them to a Thermo Fisher Scientific authorized sales/service representative.
5. Do not centrifuge explosive or flammable materials or substances which could react violently
6. Because the liquid samples, the saline, etc. are used in this cell washer, your cell washer must be grounded
properly.
7. To avoid electrical shocks, do not handle the power cord or turn on or off the POWER switch with wet hands.
8. The cell washer itself may move if the rotor fails during high-speed rotation. Make sure there is a 30 cm area
around the cell washer that will allow for such movement and do not allow individuals to enter into that area
during operation. Also do not place dangerous objects such as flammable or explosive materials on top of the
cell washer or in the surrounding area.
9. While the rotor spins, never unlock the door.
10. Repairs, disassembly, and other modifications to the cell washer are strictly prohibited unless performed by a
Thermo Fisher Scientific authorized sales/service representative.

8 | CW3 Thermo Scientific

 Precautions

C A U T I ON

1. Turn off the cell washer at the main switch. The mains plug must be freely accessible at all times.
Press STOP to shut the cell washer down.
Pull out the power supply plug or disconnect the power supply in an emergency.
2. Use the cell washer only with correct installed rotor and distributor assembly and correct inserted bowl, splash
guard and drain cover.
3. Do not move or relocate the cell washer while the rotor spins.
4. Do not lean on the cell washer.
5. Do not pour any solution such as water, detergent, or disinfectant directly into the rotor chamber. If you do so, the
bearings of the drive unit might corrode or deteriorate.
6. Connect the saline tank to the pump inlet connector of the cell washer correctly with the hose bands (standard
accessories). Otherwise the liquid might leak from the tube and the liquid might get into the inside of the cell
washer. If you think liquid is inside of the cell washer, contact a Thermo Fisher Scientific authorized sales/service
representative to clean and dry the cell washer.
7. Before you operate the cell washer, remove any dropped objects and tube fragments from inside the rotor
chamber.
8. Always check for corrosion and damage on the rotor before using it. Do not use a corroded or damaged rotor.
9. Be sure to use the specified test tubes with a wall thickness of 1 mm or more. When using thin test tubes such
as disposable test tubes, do not use them repeatedly. If any scratches, crack, inside warp, etc. is found on the
test tubes, do not use them because they cannot bear a centrifugal force.
10. Be sure to load test tubes to all the holders.
11. Use one or two drops (about 50 μl) of 3-5% erythrocyte suspension as sample volume for blood cell washing.
When using precipitated erythrocyte layer, use one or two drops (about 50 μl or less) per test tube. Use the 80%
or less of the test tube capacity as sample volume for centrifugation.
12. If you observe some abnormality in this product, stop using it immediately and contact a Thermo Fisher Scientific
authorized sales/service representative. Notify the service representative of the alarm code if displayed.
13. If the cell washer will not be used for a long time, remove the power cord from the socket.
14. For connection to a different outlet, the power cord might be needed to be replaced.
15. Follow local electrical codes.
16. Depending on the magnitude, an earthquake might damage the cell washer.
If you observe some abnormality, contact a Thermo Fisher Scientific authorized sales/service representative.

NOTICE

Usually the control panel and the surface of the cell washer get warm during operation.

Thermo Scientific CW3 | 9

Symbols Used on the Cell Washer

1. 4. Symbols Used on the Cell Washer

This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your environment safe.

This symbol refers to information on hazards, described within the manual.

This symbol demands to disconnect mains before transporting or servicing the cell washer.

This symbol demands to not pour the open cell washer with water.

This symbol shows the inflow and draining directions of the cell washer. The arrow pointing towards the cell
washer shows the inflow direction. The arrow pointing away from the cell washer shows the draining
direction.

This symbol demands to make sure, that the drain cover is installed in the cell washer door before you start
the CW3 cell washer. If not, biological hazard is possible when contaminated samples are used.
See “5. 3. 1. Drain Cover” on page 37 for removing and installation.

Caution: Federal law restricts this device to sale by or on the order with a qualified clinical facility manager or
equivalent.

10 | CW3 Thermo Scientific

 Symbols Used in the Manual

The CE mark states that this product is meeting all requirements for the European Economic Area.

This CSA mark states that this product is meeting all requirements for Canada and USA.

This symbol marks the manufacturer of this product.

This symbol marks the manufacturing date of this product.

This symbol marks the catalogue number of this product.

This symbol marks this product as intended to be used as an in vitro diagnostic medical device.

1. 5. Symbols Used in the Manual

This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your environment safe.

Thermo Scientific CW3 | 11

Symbols Used in the Manual

2. Technical Specifications
Environmental Conditions Altitudes of up to 2000 m above sea level
Max. relative humidity 80% up to 31 °C;
decreasing linearly up to 50% relative humidity at 40 °C
Environmental Conditions Temperature: -10 °C to +55 °C
during Storage and Shipping Humidity: 15% to 85%
Permissible Ambient Temperature
+2 °C to +35 °C
during Operation

Average Heat Dissipation 54 Wh / 184 Btu/h / 194 kJ/h

Overvoltage Category II

Pollution Degree 2

IP 20

Running Time Automatic: 1 – 99 sec / manual: 1 – 999 sec

Maximum Speed² nmax 3000 rpm

Minimum Speed³ nmin 330 rpm

Maximum RCF-Value at nmax 1180 x g

Noise Level at Maximum Speed¹ < 53 dB (A)

Maximum Kinetic Energy 0.46 kJ

Dimensions
Height (open door / closed door) 630 mm / 410 mm
Width 370 mm
Depth 450 mm
Weight ²
28 kg
1
Front Side Measurement, 1 m in front of the instrument at 1.6 m height. 3 washing-cycles each with about 35 s centrifugation.

2
Mode manual centrifuge

2
Mode manual decant

12 | CW3 Thermo Scientific

 Directives and Standards

2. 1. Directives and Standards

Region Directive Standard
Europe 98/79/EC In Vitro Diagnostic Medical EN 61010-1
220-230 V, 50/60 Hz Devices (IVD) EN 61010-2-020
2014/35/EU Low Voltage (protective goals) IEC 61010-2-101
2006/42/EC Machinery (protective goals) EN 61326-1 Class B
2014/30/EC Electromagnetic Compatibility EN ISO 13485
(EMC) (protective goals)
2011/65/EC RoHS – Directive on the
Restriction of the use of certain Hazardous
Substances in electrical and electronic
equipment
USA & Canada FDA – 510(k) cleared CAN/CSA-C22.2 No. 61010-1-04
120 V, 60 Hz Regulation Name: Automated Cell-Washing UL Std. No. 61010-1
Centrifuge for Immunohematology CAN/CSA-C22.2 No. 61010-2-020-09- Part 2-020
Regulatory Class: 2 IEC 61010-2-020
Product Code: KSN IEC 61010-2-101
China IEC 61010-1
220-230 V, 50/60 Hz IEC 61010-2-020
IEC 61010-2-101
EN 61326-1 Class B

2. 2. Mains Supply
The following table contains an overview of the electrical connection data. This data is to be taken into consideration, when
selecting the mains connection socket.

Unit Thermo Scientific CW3 cell washer

Article No. 75007404 75007405

Mains Voltage 120 V 220-30 V

Frequency 60 Hz 50 / 60 Hz

Rated Current 2.7 A 1.5 A

Power Consumption 135 W 135 W

Equipment Fuse 10 AT 10 AT

Building Fuse 15 AT 16 AT

Thermo Scientific CW3 | 13

Location and Function of Parts

2. 3. Location and Function of Parts

Front view
11
1

3
10
4
9

5
8

No. Part Description

1 Nozzle Through this nozzle, saline is supplied from the pump to the distributor.
2 Drain cover Receives the waste liquid decanted from the rotor and discharges it to the outside.
3 Distributor Distributes the saline supplied from the pump to each test tube held on the rotor.
4 Rotor A 12 or 24-sample rotor.
5 Splash guard Designed to keep saline, waste liquid, etc. from leaking to the internal mechanism of the
cell washer. Removable.
6 Condition-setting panel See “2. 5. Condition-setting Panel” on page 17.
7 POWER switch Switches on and off the cell washer power. It doubles as a circuit protector. It
automatically switches off the cell washer power if abnormal current passes.
8 Control panel See “2. 4. Control Panel” on page 16.
9 Door lock lever Keeps the door locked while the rotor is running.
10 Bowl Rotates together with the rotor. It determines the angle to which the test-tube holders of
the rotor swing.
11 Door Test tubes are set on the rotor after opening the door. The drain cover and nozzle are
installed on the back of the door.

14 | CW3 Thermo Scientific

 Location and Function of Parts

Rear view
1

2 5

3 4

No. Part Description

1 View port You can measure the rotor speed through the view port by an optical reflection type
rotation meter.
2 Drain connector The overflowed waste liquid is drained through this connector.
3 Power connector To be connected to a power supply.
4 Sensor connector To be connected to the sensor which detects the level of saline in the tank.
5 Auxiliary drain tube The chamber is drained through this tube if the drain connector is blocked.
6 Pump Provided for supplying saline into the cell washer.
7 Pump inlet connector To be connected to the saline tank by tubing.
8 Door stopper parts Designed to keep the open angle (60°) of the door to prevent the door to be wide-open.

Thermo Scientific CW3 | 15

Control Panel

2. 4. Control Panel

10
1
9
8
7
6
2

3 4 5

No. Name Function

1 PROCESS LED Shows the current process of blood cell washing. Indicates the process setting in manual
mode. While the cell washer operates, the LED of the active process blinks.
2 CYCLE indicator Indicates the number of washing cycles. While the cell washer operates, the CYCLE
indicator shows the number of the remaining cycles.
3 MANUAL switch To manually start a single process (WASH, CENTRIFUGE, DECANT or AGITATE). The LED
lights when the manual mode is set.
4 OPEN switch To open the door. If it is pressed continuously, the cell washer does not accept any input
of the other switches for 4 seconds (alarm buzzer sounds).
5 STOP switch To stop a process. It is also used for stopping the end buzzer and alarm buzzer. When the
operation is stopped in the automatic mode or in the agitation/centrifugation (see No. 9),
the operation can be restarted at the stopped point by pressing the START switch. When
the operation is stopped in the manual mode, the operation is not restarted at the
stopped point by pressing the START switch.
The LED lights when the operation is stopped. When the operation is stopped in
automatic mode, its LED blinks.
6 AGI/CENT switch Sets the agitation time (agitation of liquid in test tubes) and the centrifugation time
(manual mode) (see the figure below). Its LED lights when it is set.
Operation is started when START is pressed.

Manual-mode set speed

1 2

1 Agitation set time

2 Manual-mode centrifugation time

This cell washer does not accept the input of START when the agitation set time is
0 second.
7 START switch To start a series of processes or a single process.
8 FEED switch In automatic mode, it causes the value of washing cycle setting to count down. In manual
mode, it is used for settings desired for one of the processes.
9 AUTO switch To start automatic washing cycles. The LED lights when the automatic mode is set.
10 TIME indicator Indicates the remaining time of centrifugation (in 3 digits). In case of an abnormality, the
proper error code is shown. It also shows the rotor speed (×10 rpm) when PUSH SPEED
on the condition-setting panel is pressed.

16 | CW3 Thermo Scientific

 Condition-setting Panel

2. 5. Condition-setting Panel

1 2 3 4 5 6 7 8 9 10 11 12

No. Name Function

1 CYCLE setting switch Sets the number of the washing cycles to be repeated (9 cycles at maximum).
2 AUTO CENTRI TIME setting switch Sets the centrifugation time of automatic-mode operation (99 seconds at
maximum). The standard centrifugation time is 35 seconds.
3 MANUAL CENTRI TIME setting switch Sets the centrifugation time of manual-mode operation (999 seconds at
maximum).
4 MANUAL SPEED setting switch Sets one of the following rotor speeds for manual-mode centrifugation:
3,000 rpm (High) and 1,200 rpm (Low)
5 DECANT SPEED setting switch Sets the rotor speed for automatic- or manual-mode decantation using the H/L
switch and adjusting a number. If the number is set between 4 and 9, the rotor
speed is the same as if 0 is set.

Number
Switch
0 1 2 3
H 350 rpm 400 rpm 450 rpm 500 rpm
L 330 rpm 370 rpm 410 rpm 450 rpm
Decantation is completed in a short time. Note that the above value may not
necessarily match the speed indicated by the PUSH SPEED switch.
If you increase the DECANT SPEED, the amount of the remained blood cells
tends to decrease.
If you decrease the DECANT SPEED, the amount of the remained blood cells
tends to increase.
6 PUSH SPEED switch Keep the PUSH SPEED switch pressed when the cell washer started spinning.
The actual spinning speed of the rotor will be shown (×10 rpm) on the TIME
indicator on the control panel. Example: 1200 rpm are shown as 120 on the
TIME indicator (120 x 10 = 1200).
7 OVERFLOW setting switch When the pump is set to overflow the cell washer will overflow the inserted
tubes with solution from the saline tank.

Setting Pumping operation for overflow

0 No operation
1 Operates only the first cycle.
2 Operates only the first and the second cycles.
3 Operates only the first, second and the third cycles.
4 Operates only the first, second, third and the fourth cycles.
5 Operates only the first, second, third, fourth and the fifth cycles.
6 to 9 Selectable up to 9 in the same manner.

Thermo Scientific CW3 | 17

Condition-setting Panel

No. Name Function

8 PROGRAM switch Sets the operation program.

Setting Program (No setting more than 1)

0 5-second centrifugation is not performed at the final process of
the final cycle in automatic mode.
1 5-second centrifugation is performed at the final process of the
final cycle in automatic mode.
2 You can add the conditions to the centrifugation process at
manual mode:
ƒƒ “3000 rpm for 15 seconds”: “H” is displayed on the
CYCLE indicator and “15” is displayed in the TIME
indicator.
ƒƒ “1200 rpm for 60 seconds”: “L” is displayed on the
CYCLE indicator and “60” is displayed in the TIME
indicator.
See the following procedure:

1. Press MANUAL.
The condition displayed on the condition-setting panel
is performed.
2. Press FEED.
Centrifugation (3000 rpm for 15 seconds) is performed.
3. Press FEED again.
Centrifugation (1200 rpm for 60 seconds) is performed.

5-second centrifugation is not performed at the final process of

the final cycle in the automatic mode.
(If the dial is set between 3 and 9, the operation program is the same as that of
dial setting 0.)
9 AGITATE TIME setting switch Sets the agitation time (99 seconds at maximum).
10 MELODY setting switch Sets the end buzzer.

Setting Tunes
0 No sound
1 Electronic beep (three beeps)
2 Oh Susanna
3 My Bonnie
4 My Old Kentucky Home
5 De Camptown Races
6, 7, 8 or 9 Electronic beep (two beeps)
You can turn down the sound level by covering the hole with an adhesive tape
(this hole is above the right side of the CYCLE setting switch).
11 OPTION switch This is a push-button switch for saline calibration purpose.
12 SALINE PRIME switch To be used for pump bleeding at the time of initial operation. The pump for
saline supply operates while this switch is kept depressed.
This switch is ineffective while the cell washer is in operation.

18 | CW3 Thermo Scientific

 Before Setting Up

3. Transport and Set Up

3. 1. Before Setting Up
1. Check the cell washer and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher
Scientific immediately if any damage is discovered.
2. Remove the packaging.
3. Check, if the items supplied are complete. „Items Supplied“ on page 5.
If the items supplied are incomplete, please contact Thermo Fisher Scientific.

3. 2. Location
WARNING The cell washer is neither inert nor protected against explosion. Never use the cell washer in an explosion-
prone environment.
WARNING UV rays reduce the stability of plastics. Do not subject the cell washer, rotor and plastic accessories to direct
sunlight.
The cell washer is only to be operated indoors.
The set-up location must fulfill the following requirements:
ƒƒ Set up the cell washer in a room where the ambient temperature is 5 to 35 °C.
ƒƒ A safety zone of at least 30 cm must be maintained around the cell washer.
People have to stay out of this safety zone while centrifuging.
ƒƒ The supporting structure must be stable and free of resonance.
ƒƒ The supporting structure must be suitable for horizontal setup of the cell washer.
ƒƒ The set-up location must be well-ventilated at all times.
ƒƒ The cell washer is not to be exposed to heat and strong sunlight.

3. 3. Transporting
Due to its weight, the cell washer should be carried by at least two people. Always lift the cell washer at both sides.
To prevent possible injuries at least two people should lift and carry the cell washer by holding it at the bottom from
opposite sides.
WARNING Always lift the cell washer from both sides. Never lift the cell washer by its front panel, its back panel or at its
door. Always remove the rotor before moving the cell washer.

Transport the cell washer and accessories upright within the associated packaging, if possible.
NOTICE The original cell washer packaging is a one way packaging. Only keep the two styropor pieces for setting up
the cell washer (“3. 6. Setting Up” on page 20). Do not keep the rest of the one way packaging. Contact a shipping
company or the customer service for the transport. Always remove the rotor before moving the cell washer. If you do not
remove the rotor you might damage the cell washer drive or cell washer spindle.

Thermo Scientific CW3 | 19

Leveling

3. 4. Leveling
C A U T I ON
If the cell washer is not leveled, imbalances can occur and the cell washer can be damaged.
Do not place anything under the cell washer feet to level the cell washer.

The cell washer is to be placed in horizontal and level supporting structures or benching.
Horizontal level is to be checked when moving the cell washer to a new location.

3. 5. Mains Connection
NO T I C E
Plug the cell washer in grounded electrical sockets only.

1. Turn off the power supply switch.

2. Check whether the cable complies with the safety standards of your country.
3. Make sure that the voltage and frequency correspond to the figures on the rating plate.

3. 6. Setting Up
NO T I C E
Setting up the CW3 Cell Washer is easier when done by 2 people.

1.  Install the motor guard plate

1. Lift the front bottom of the cell washer. Put the two styropor pieces from the one way cell washer packaging
below the cell washer feet. Carefully put the cell washer on the styropor pieces.

2. Loose the wing nuts counterclockwise. ➀

20 | CW3 Thermo Scientific

 Setting Up

3. Remove the motor holder. ➁

➁ ➁

➀ ➀

4. Place the motor guard plate. ➀

5. Secure the motor guard plate with the wing nuts clockwise. ➁

➀ ➀

➁ ➁

NOTICE Keep the removed motor holder for future transportation.

2.  Connect power cable

Connect the power cord to the power connector at the rear of the cell washer.
Plug the power cord in the socket.
Check for grounding.

3.  Connect sensor cable

Connect the sensor cable from the top of the tank with the sensor connector at the backside of the cell washer. (See
the green highlighted sensor cable on the picture in „Connect tubing“ on page 26)

4.  Switch on cell washer

Switch on the power supply switch.
The liquid level sensor in the tank activates. Error code “E2” (the saline tank needs to be refilled) is indicated and the
buzzer sounds during the power-on. The buzzer stops as STOP is pressed.

5.  Open lid
Press OPEN and open the door.

6.  Switch off cell washer

Switch off the power supply switch.

Thermo Scientific CW3 | 21

Setting Up

7.  Remove packaging
Remove the packagings from the rotor chamber.

8.  Remove the drain cover

1. Slide the pins in direction of the arrows at both sides.

2. Pull the drain cover forward to remove it.

9.  Remove the splash guard

Remove the splash guard from the rotor chamber.

22 | CW3 Thermo Scientific

 Setting Up

10.  Check rubber gasket

Make sure that the rubber gasket is in position. The rubber gasket must cover the base of the decantation coil.

To get the rubber gasket in position: push the decantation coil in one direction and push the rubber gasket in the
opposite direction where it is not in correct position.
If the rubber gasket is not in position, fluids can damage the cell washer.

11.  Install splash guard

Install the splash guard in the rotor chamber.

Thermo Scientific CW3 | 23

Setting Up

12.  Install drain cover

1. Insert the drain cover into the drain cover holder to install it.

2. Push the pins in the door safely until a click is heard.

CAUTION When installing the drain cover, check that the nozzle at the center of the door is put through the hole of
the drain cover. Install the drain cover correctly.

13.  Install bowl
1. Apply silicone grease (76003500) to the inside of the bowl and the o-ring of the decantation coil lightly.

2. Put both hands on the inside of the bowl and push down the bowl horizontally.

3. When the bowl is pushed about one-third or a half of the bowl height, put both hands and push down the bowl
furthermore turning the bowl until the bottom of the decantation coil becomes even with the bottom of the bowl.
Check that they are even by passing fingers over the surface.

4. The bowl must be correctly installed and align with the decantation coil before performing a test run.
If not, the door lock will not work, the door cannot be opened, the bowl will contact the drain cover and the
cell washer will be out of order.
If you set the bowl correctly, the tube holders will touch the metal part of the bowl when the rotor is installed.
Check that the bowl can be turned freely. If the bowl can not be turned freely, the center packing and/or the rubber
gasket might not be mounted properly.

24 | CW3 Thermo Scientific

 Setting Up

14.  Install rotor
1. Remove the rubber bands from the rotor.

2. Set the rotor on the drive shaft.

3. Turn the rotor about a quarter so that the coupling pins are inserted into the rotor shaft holes.

4. Turn the top of the rotor clockwise and counterclockwise to check that the coupling pins are inserted into the
rotor shaft holes (no free turn) after setting.

CAUTION If the rotor is not set correctly, the rotor holder may come off the bowl and overswing.

Thermo Scientific CW3 | 25

Setting Up

15.  Install distributor
Set the distributor on the top of the rotor.

Make sure that the pins at the bottom of the distributor move in the holes at the top of the rotor.

16.  Connect tubing

➅
➃

➂
➁ ➄ ➀

➀ Saline tank

➁ Waste drain

➂ Sensor cable

➃ Drain tube

➄ Gray outlet

➅ Inlet tube

1. Put the saline tank at a place higher than the cell washer or behind the cell washer. ➀

2. Put the waste drain at a place lower than the cell washer. ➁
Make sure that the waste drain is lower than the cell washer to prevent backward flow of waste liquid.

26 | CW3 Thermo Scientific

 Setting Up

Prepare the saline tank and a drain for waste following your laboratory standards. A waste liquid tank is
shown as an example in the picture.

3. Connect the sensor cable of the saline tank to the connector at the rear of the cell washer. ➂

4. Connect the drain tube or the L-shaped adapter, which can be connected to the drain tube, to the drain connector. ➃
Apply a thin layer of silicone grease (76003500) to the drain connector. Do not force the drain tube or the
L-shaped adapter into the drain connector.
Tighten it with the hose band (big).
Hold the L-shaped adapter facing downward and connect it to the drain connector. If not waste liquid can
stow and result in bad drainage.

✓
Make sure to position the L-shaped adapter correctly.

Place the end of the drain tube in the waste liquid tank or a sink. Position them higher than the liquid level.

5. The gray outlet at the rear center of the cell washer is used for draining off waste liquid from the chamber. ➄
After blood cell washing, waste liquid keeps draining off through the connector. The auxiliary drain tube under
the connector is to drain waste liquid flowed from the chamber into the vicinity of the driving motor. Waste liquid
may not always drain off through it. Prepare a drain or tank for waste liquid coming out of the auxiliary drain
tube of the rear of the cell washer when operated. Instead of preparing an additional drain or tank for waste
liquid, you may lengthen the tube (You can use the rest of the tube of the tank). You can insert the tube in the
waste liquid drainage you use for the drain tube.
CAUTION Make sure that the tips of the drain tubes are above the waste liquid level in the tank. Otherwise, the
chamber cannot be drained completely and waste liquid stagnates in the chamber because of poor drainage. Poor
drainage can also be caused if the tube is bent or flattened or if waste liquid stagnates in the middle of the tube.
Set the tubes properly so that waste liquid drains off smoothly. Be careful not to set the drain tubes at a place higher
than the drain outlet of the cell washer.

6. Connect the saline tank to the pump inlet connector of the cell washer. ➅
Connect the tubing to the pump inlet connector. Tighten it with the hose band (medium).
Connect the tubing with the nozzle of the tank carefully. Tighten it with the hose band (small).
If it is not connected using the hose bands liquid can leak from the tube and might get inside of the cell
washer. If the liquid might have leaked into the inside of the cell washer, contact a Thermo Fisher Scientific
authorized sales/service representative for cleaning and drying the cell washer.

Thermo Scientific CW3 | 27

Storage

3. 7. Storage
WARNING If necessary clean, disinfect or decontaminate the entire system when removing the cell washer and
accessories from use. In doubt contact the Thermo Fisher Scientific customer service.
ƒƒ Before storing the cell washer and the accessories, it must be cleaned and if necessary desinfected and decon-
taminated.
ƒƒ Cell washer and accessories have to be thoroughly dried before storage.
ƒƒ Store the cell washer in a clean and dust-free location.
ƒƒ Be sure to place the cell washer on its feet.
ƒƒ Avoid direct sunlight.

3. 8. Shipping
WARNING Before shipping the cell washer and accessories you have to clean and if necessary disinfect or decontaminate
the entire system. In doubt contact the Thermo Fisher Scientific customer service.
Before shipping the cell washer please bear the following in mind:
ƒƒ The cell washer must be clean and decontaminated.
ƒƒ The decontamination must be confirmed in a decontamination certificate (“Decontamination Information
Certificate” on page 42). Contact customer service for more details.
ƒƒ Install the motor holder for shipping.

28 | CW3 Thermo Scientific

 Preparation

4. Operation
WA R N I NG
Use the cell washer only with correct installed rotor, distributor assembly and correct inserted bowl, splash guard
and drain cover.
Hazardous substances can leak out of the cell washer, if bowl and drain cover are missing.

C A U T I ON
Open the door carefully after blood cell washing. Otherwise the remaining waste liquid from the inside of the drain
cover might splash.

C A U T I ON
Close the door by pushing down at the forward center of the door until a short beep is heard.
If the door is closed insufficiently, an alarm (E1: DOOR OPEN) is indicated and the cell washer does not start opera-
tion. If this alarm is indicated, close the door and press START again.

4. 1. Preparation
1. Prepare the test tubes (10 or 12 mm in diameter and 75 mm in length). When using the test tubes 10 mm in diam-
eter, set the attached D10 adapter to the rotor holder. Make sure that the brim of the adapter is pointing outwards.
CAUTION Be sure to use specified test tubes with a wall thickness of 1 mm or more. When using thin test tubes
such as disposable test tubes, do not use them repeatedly. If any scratch, crack, inside warp, etc. is found on the
test tubes, do not use them because they cannot bear a centrifugal force. It is recommended to use tempered hard
glass test tubes for this cell washer.
2. Check and prepare the saline tank and a drain for waste following your laboratory standards.
CAUTION Make sure that the tips of the drain tubes are above the waste liquid level in the tank. Otherwise, the
chamber cannot be drained completely and waste liquid stagnates in the chamber because of poor drainage. Poor
drainage can also be caused if the tube is bent or flattened or if waste liquid stagnates in the middle of the tube.
Set the tubes properly so that waste liquid drains off smoothly. Be careful not to set the drain tubes at a place higher
than the drain outlet of the cell washer.

4. 1. 1. Injection Volume Adjustment and Pump Ventilation

Adjustment of saline injection volume
The CW3 cell washer is factory-adjusted to the injection volume of saline for use with the 24 place rotor and the 12 mm
diameter test tubes.
When using the 24 place rotor and 12 mm diameter test tubes with this cell washer, there is no need to adjust the injec-
tion volume. When using the 12 place rotor or 10 mm diameter test tubes, adjust the flow rate of the pump as follows:
1. Remove the protective cover of the pump at the rear of the cell washer (it is secured with two screws).
2. Loosen the two locking screws of the pump counterclockwise.
3. Turn the adjusting screw to set the indicator value appropriate to your rotor and tube settings.
4. Tighten the two locking screws clockwise.
Reference (Index of injection volume)

Thermo Scientific CW3 | 29

Preparation

5. Reinstall the protective cover of the pump.

Rotor Test tube Injection volume Indicator setting

(+/- 10%) ➁
➀
12 place 12 mm diameter 48 ml 45 %
12 place 10 mm diameter 32 ml 30 %
24 place 12 mm diameter 96 ml 80 %
24 place 10 mm diameter 64 ml 60 %
➂

➀ Adjusting screw
➁ Indicator (red dot)
➂ Locking screws

6. Saline calibration
a. Press OPTION with a beaker held against the nozzle. The saline pump runs for 5 seconds.
b. Compare the preset volume in the above table and the actual volume injected in the beaker.
c. Set the adjusting dial screw when the actual volume is not in the preset volume range.
Repeat step a and b until calibration is succesful.

Pump ventilation
Fill the saline tank with saline. Hold a beaker or other container to the nozzle. Press SALINE PRIME on the condi-
tion-setting panel to discharge saline from the nozzle until no bubbles come out of the nozzle.

30 | CW3 Thermo Scientific

 Preparation

4. 1. 2. Setting the Operating Conditions

The operating conditions are factory-set as follows.
Setting dials and switches Factory-setting
1 CYCLE 3 cycles
2 AUTO CENTRI TIME 35 seconds
3 MANUAL CENTRI TIME 60 seconds
4 MANUAL SPEED L (1200 rpm)
5 DECANT SPEED H-1 (400 rpm)
6 OVERFLOW 1 (Operates only the first cycle)
7 PROGRAM 1 (5-second centrifugation is performed at the final cycle in automatic mode)
8 AGITATE TIME 5 seconds
9 MELODY 1 (Electronic beep (three beeps))
Agglutination of blood cells is influenced by centrifugal force and centrifugation time. If centrifugal force and centrifugation
time are insufficient, agglutination can be insufficient causing incorrect results. Excessive centrifugation can harden the
blood cells causing incorrect agglutination or difficult resuspension. The weak reaction may disappear. Centrifugal condi-
tions are influenced by the amount of blood cells, specific gravity of blood cells, etc. The above settings are just a guide.
Determine the optimum conditions according to the following evaluation.
1. The supernatant is transparent and no blood cell is suspended after centrifugation.

2. The blood cells that are precipitated at the bottom of the test tubes show clear outlines after centrifugation.

3. The blood cells are easily removed from the bottom and disentangled by a light agitation after decantation.

4. Check the reaction using reagents whether negative or positive.

5. Check the amount of the remained blood cells. If the amount of the remained blood cells is small, lower the
DECANT SPEED.

Thermo Scientific CW3 | 31

Preparation

4. 1. 3. Preparation of the Sample

C A U T I ON
When the sample or liquid spilled, it can leak into the cell washer.
If the sample or liquid leaked into the cell washer, contact Thermo Fisher Scientific service for cleaning and drying.

Blood cell washing

Use one or two drops (about 50 μl) of 3-5% erythrocyte suspension in one test tube.
CAUTION When centrifuging precipitated erythrocyte layer, use one or two drops (about 50 μl or less) per test tube. If not
a series of blood cell washing processes can be not complete.

Centrifugation
Use the 80% or less of the test tube capacity as sample volume for centrifugation. The tubes are centrifuged at an angle

of 38.5°.

1 2

38.5°

1 Centrifugal force
2 Drive shaft

32 | CW3 Thermo Scientific

 Operation Modes

4. 2. Operation Modes
4. 2. 1. Automatic Mode
Step Operation Description
1 Switch on AUTO. AUTO lights up.
The automatic mode is selected automatically when the power switch is
switched on.
2 Switch on START. START lights up.
To stop the cell washer temporarily in the middle of a process, press the STOP
switch and the cell washer stops at once.
For restarting the process, press the START switch.
3 Upon finish of all processes The end buzzer sounds and START lights off.
The door is opened.
The buzzer stops as STOP is switched on.

NOTICE In automatic mode, the set number of cycles can be decreased if you push FEED. For example, if three cycles
are set, the number changes 321 as FEED is pressed.

4. 2. 2. Manual Mode
In manual mode the process steps (WASH, CENTRIFUGE, DECANT, AGITATE) can be run as single process steps. More
information on the single process steps: „Operational Sequence for Antiglobulin Test“ on page 34.
Step Operation Description
1 Switch on MANUAL. MANUAL lights up.
2 Press FEED once or multiple times to set The CENTRIFUGE process is set preferentially.
the desired process manually. Possible process steps:
The LED of the set PROCESS lights up.
1. DECANT - Pressing FEED once (1x) will set the DECANT process. The
according LED lights up.
2. AGITATE - Pressing FEED twice (2x) will set the AGITATE process. The
according LED lights up.
3. WASH - Pressing FEED three times (3x) will set the WASH process. The
according LED lights up.
4. CENTRIFUGE - Pressing FEED four times (4x) will set the CENTRIFUGE
process as set preferentially when MANUAL is pressed. The according LED
lights up.
Make sure that the desired values on the condition-setting panel for the
manually set process step are set.
3 Switch on START. START lights up.
Upon finish of the selected process The end buzzer sounds and the door is opened.
START lights off.

NOTICE The door is automatically opened after the rotor is stopped by pressing STOP in the middle of operation.
NOTICE When power fails during operation and then it is restored, or when POWER is switched off during operation and
then turned on again, the automatic-mode operation is set (AUTO lights up) and the cell washer stops. In such cases, set
the desired process again and perform operation in the manual mode.

4. 2. 3. Agitation-Centrifugation
Step Operation Description
1 Switch on AGI/CENT. AGI/CENT lights up.
2 Switch on START. START lights up.
To stop the cell washer temporarily in the middle of a process, press STOP. For
restarting the process, press START.
3 Upon finish of the process The end buzzer sounds and START lights off.
AGI/CENT lights up and the door is opened.

Thermo Scientific CW3 | 33

Operation Modes

4. 2. 4. Operational Sequence for Antiglobulin Test

3000
➊ Rotor speed (rpm)
➎ ➋ Process (details below)
➊ 1200
➌ 1 cycle
400

➋      ➍ Time
➌ ➎ Injection of saline
➍

Reference
Process Operation Description
Illustration
 WASH A fixed amount of saline is pumped into the
distributor when the rotor speed reaches
1200 rpm. The saline is injected by centrifgual
force from the distributor into the test tubes. The
blood cells in the test tubes are sufficiently
suspended in the saline.

 CENTRIFUGE Blood cells are centrifuged.

The standard centrifugation time is 35 sec.
(selectable). Before the rotor decelerates, the
injection of saline continues to overflow the test
tubes.
(Overflow is also selectable.)

 DECANT The rotor spins at low speed with the rotor holder
kept at an angle to slightly open its top end by
repeated 3 or 4 times

magnetic force. By this operation only the saline

Washing cycle

decants from the test tubes and the blood cells

remain.

 AGITATE The rotor repeats rotation and stops at short, quick

intervals to disentangle the remaining blood cells.

 CENTRIFUGE The rotor spins about 5 seconds to collect the

blood cells adhered to the wall surfaces of the test
tubes at the bottom. This is done to ensure the
reaction with the Antiglobulin reagent. This
operation is performed at the end of the washing
cycle.

34 | CW3 Thermo Scientific

 Operation Modes

5. Maintenance
WA R N I NG
Cell washer, rotor or accessories can be contaminated by samples.
Decontaminate according to good laboratory procedures and methods.

WA R N I NG
If there is a possibility that the cell washer, rotor, or an accessory is contaminated by samples that might impair
human health (for example, samples that are toxic or radioactive, or blood samples that are pathogenic or infectious),
it is your responsibility to sterilize or decontaminate the cell washer, rotor, or the accessory correctly before request-
ing repairs from Thermo Fisher Scientific service. Note that Thermo Fisher Scientific can not repair the cell washer,
rotor or the accessory unless sterilization or decontamination is completed.

WA R N I NG
It is your responsibility to sterilize and/or decontaminate the cell washer, rotor, or parts correctly before returning
them. In such cases copy the decontamination sheet at the end of this manual, fill it out and attach it to the item to
be returned. Thermo Fisher Scientific may ask you about the treatment for the cell washer, rotor or the part if the
decontamination is checked and judged as insufficient by Thermo Fisher Scientific. It is your responsibility to bear the
cost of sterilization or decontamination. Note that Thermo Fisher Scientific can not repair or inspect the cell washer,
rotor or the accessory unless sterilization or decontamination is completed.

C A U T I ON
Do not operate the cell washer in any manner not described in this instruction manual. Should you have any troubles
with the cell washer, call Thermo Fisher Scientific service.

C A U T I ON
Refrain from using any other cleaning or decontamination procedure than those recommended here, if you are not
entirely sure that the intended procedure is safe for the equipment.
Use only approved cleansers.
If in doubt, contact Thermo Fisher Scientific.

Thermo Scientific CW3 | 35

Pump, Tank and Tubing

5. 1. Pump, Tank and Tubing

ƒƒ Wash the inside of the tank and the tubing once a week. If saline is contaminated by bacteria, it may cause
hemolysis or poor result.
ƒƒ If the cell washer is not used for a long period, drain out saline from the tank, tubing and pump.
ƒƒ Check the volume of saline inside the tank before operation. Connect the tube securely to the saline tank and
the pump inlet connector with the hose bands. When the tube is loose, replace it.
ƒƒ Replace the drain tube and the tube every 1 to 3 years depending on the degree of discoloration.

Washing
1. Fill up the saline tank with washing solution (0.5% Sodium Hypochlorite Solution).

2. Fill up the fluid passage with washing solution according to the same procedure as pump ventilation (“4. 1. 1.
Injection Volume Adjustment and Pump Ventilation” on page 29) and leave it for 12 to 24 hours.

3. After 12 to 24 hours drain out the washing solution from the saline tank and fill up the tank with distilled water.

4. Flow distilled water 2 to 3 liters from the saline tank according to the same procedure as pump bleeding.

5. Exchange distilled water in the saline tank with saline.

6. Flow saline 2 to 3 liters from the saline tank in the same manner as described in point 4.
It is recommended to use saline that includes antiseptics.
The pump is a replacement part. Liquid may leak from the bellows of the pump because of deterioration. Deterioration
depends on the installation environment such as ultraviolet rays and temperature. We highly recommended to replace the
bellows of the pump (S413230A) every three years.

5. 2. Rotor, Distributor and Bowl

After operation remove the rotor, distributor and bowl. Wash and dry them well.
See the following table for cleaning.
Part Cleaning
Rotor Soak in 0.5% sodium hypochlorite solution. Wash and dry.
Distributor, bowl Soak in a 0.5% sodium hypochlorite solution (within 1 hour). Wash and dry.

CAUTION The distributor is made of polycarbonate. For decontamination make sure that you use a 0.5% sodium
hypochlorite solution or the distributor might deteriorate. Use neutral detergent (pH 5 to 9). Do not dip the distributor in
the diluted detergent solution for a long time or the strength of the distributor can decrease.

5. 3. Chamber, Splash Guard, Drain Cover and

Door Stopper Parts
Remove the splash guard and the drain cover from the cell washer. Wash and dry them well.
Use a 0.5% sodium hypochlorite solution for cleaning. When washing the inside of the chamber, be careful not to pour
washing solution on the decantation coil. Wipe the decantation coil with a cloth or paper moistened with washing solution.

36 | CW3 Thermo Scientific

 Chamber, Splash Guard, Drain Cover and Door Stopper Parts

5. 3. 1. Drain Cover
Removing
1. Slide the pins in direction of the arrows at both sides.
2. Pull the drain cover forward to remove it.

Mounting
1. Insert the drain cover into the drain cover holder to install it.
2. Push the pins in the door safely until a click is heard.
CAUTION When installing the drain cover, check that the nozzle at the center of the door is put through the hole of the
drain cover. Install the drain cover correctly.

5. 3. 2. Splash Guard
Removing
1. Remove the drain cover. See “5. 3. 1. Drain Cover” on page 37.
2. Remove the distributor, rotor and bowl.
3. Remove the splash guard by pulling it up.

Mounting
1. Insert the splash guard.
2. Install the distributor, rotor and bowl. See “3. 6. Setting Up” on page 20.
3. Mount the drain cover. See “5. 3. 1. Drain Cover” on page 37.
CAUTION Do not store washing solution in the chamber. Do not spill washing solution on the decantation coil or liquid
can leak into the cell washer causing a failure.
If a test tube is broken, be sure to remove the fragments inside the chamber and the drain tube of the drain cover thor-
oughly with a brush.

5. 3. 3. Checking and Replacing the Door Stopper Parts

If you observe the below, call a service technician.
1. The rubber mount cracks or deteriorates.
2. The door keeps being wide-open after you open the door.
If the door keeps being wide-open, drops of waste liquid on the drain cover might fall on the rear of the cell washer.

Thermo Scientific CW3 | 37

Shipping and Disposal

5. 4. Shipping and Disposal

WA R N I NG
When removing the centrifuge and accessories from use for disposal
you have to clean and if necessary disinfect or decontaminate the entire system. In doubt contact the Thermo Fisher
Scientific customer service.

For the disposal of the centrifuge mind the regulations in your country. Contact the Thermo Fisher Scientific Customer
Service for the disposal of the centrifuge. For contact information check the backpage of this manual or visit www.ther-
moscientific.com/centrifuge
For the countries of the European Union the disposal is regulated by the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC.
Mind the information on transport and shipping (“3. Transport and Set Up” on page 19, “3. 8. Shipping” on page 28).

38 | CW3 Thermo Scientific

 Mechanical Emergency Door Release

6. Troubleshooting

6. 1. Mechanical Emergency Door Release

C A U T I ON
Opening the door while the rotor spins is very dangerous. Never unlock the door while the rotor spins. If the door
is opened while the rotor still spins, close the door immediately. Do not run the cell washer with the allen wrench
inserted in the hole of the cell washer.

The door can not be opened and closed except when the cell washer is switched on and the rotor has stopped. If the door
can not be opened because of a power failure, open the door according to the following procedure.
1. Check that the rotor does not spin.
Listen carefully to make sure that no spinning sound is heard.
It takes about one minute until the rotor spinning at 3,000 rpm (maximum speed) stops completely. Wait for a
sufficient time.
2. Insert the attached allen wrench into the small hole to unlock the door.
A small hole is provided on both sides of the cell washer.
Insert the attached allen wrench straight into the small hole and push it in until a click is heard. Then insert the allen
wrench into the small hole of the other side in the same manner. The door unlocks and opens.

Thermo Scientific CW3 | 39

Error Codes

6. 2. Error Codes
If any abnormality occurs during operation, corresponding error code blinks on the TIME indicator, the alarm buzzer sounds,
and the cell washer stops. Take the action described in the following table or contact Thermo Fisher Scientific service.
Error code Desciption Troubleshooting
E1 The door is open. Close the door safely and press START.
E2 The saline tank needs to be refilled. Refill the tank and press STOP to clear the error code. In the manual
mode, CENTRIFUGE and DECANT processes can be executed even when
this error code is shown.
The liquid level sensor may be faulty if the alarm buzzer sounds even
though there is enough saline. Contact a Thermo Fisher Scientific
authorized sales/service representative for repair. The error code can be
temporarily cleared by pressing MANUAL and STOP simultaneously for
three seconds or more (operation in automatic mode is possible). It returns
to the original state by turning on and off the power switch.
E3 A power failure occurs. If a power failure occurs while the cell washer is running, the cell washer
stops spinning after the power failure and the error code “E3” is shown.
In order to clear the error code, press any of the switches on the control
panel after a lapse of one minute or more from when the error code is
indicated. If the error persists, contact Thermo Fisher Scientific service.
This error can be caused by voltage drop if your cell washer is connected
to an outlet to which multiple appliances are connected when the rotor
accelerates. Turn off the cell washer power and connect the cell washer to
an outlet that can supply stable voltage to the cell washer.
E4 The power supply is incorrect. The frequency of the power supply is incorrect. Turn off the power switch
and on again. If the error persists, contact Thermo Fisher Scientific
service.
E5 The rotor overspeeds. This error code is shown when the rotor runs at a speed higher than the
specified speed. Contact Thermo Fisher Scientific service.
E6 An incorrect current flows. This error code is shown when an incorrect current flows in the circuit.
Contact Thermo Fisher Scientific service.
E7 The speed sensor is defective. The sensor to detect the rotor speed is defective. Contact Thermo Fisher
Scientific service.
E8 The current sensor is defective. The sensor to detect the current is defective. Contact Thermo Fisher
Scientific service.
E9 The RAM is defective. Turn off and on the power switch. If the error persists, the microcomputer
of the cell washer to control its operation is defective. Contact Thermo
Fisher Scientific service.
E10 The rotor overspeeds (hardware detection). This error code is shown when the rotor runs at a speed higher than the
specified speed. Contact Thermo Fisher Scientific service.
E11 The triac is defective. The element on the circuit board is defective. Contact Thermo Fisher
Scientific service.
E14 The door lock is defective. The door lock is defective. Contact Thermo Fisher Scientific service.
Others System error Contact Thermo Fisher Scientific service.

40 | CW3 Thermo Scientific

 Troublshooting when no Error Code is indicated

6. 3. Troublshooting when no Error Code is

indicated
WA R N I NG
Unauthorized disassembly of the cell washer except by Thermo Fisher Scientific service is strictly prohibited.

No. Symptom Possible cause Remedy

1 POWER does not light its LED even if it 1. A power failure occurs. 1. Wait until power is restored.
is turned on. 2. The power cord is disconnected. 2. Contact Thermo Fisher Scientific
service.
2 The cell washer does not start 1. The door is not closed. 1. Close the door.
operation. 2. The door switch is defective. 2. Contact Thermo Fisher Scientific
service.
3 Injection of saline does not take place. 1. No saline is in the tank. 1. / 2. For the the first two possible
2. The pump is not filled with causes: refill the tank with saline to
saline. fill the pump.

3. The pump is defective. 3. Contact Thermo Fisher Scientific

service.
4. The pump connector is loose.
4. Retighten it securely.
4 Saline (waste liquid) scatters in the 1. Injection volume is excessive 1. Adjust the injection volume
chamber. and the test tubes are overflown correctly.
1. The amount of blood cells after with solution. 2. / 3. Contact Thermo Fisher Scientific
washing is less than before. 2. The nozzle is out of position and service.
2. No washing is executed. fails to supply the distributor
with solution.
3. Washing is executed.
3. The drain cover is broken.
5 No decantation is executed. The coil is disconnected. Contact Thermo Fisher Scientific
service.
6 Abnormal noise is heard. 1. The bowl and the rotor contact 1. Set the bowl and the rotor correctly.
1. Contacting noise the drain cover. 2. Contact Thermo Fisher Scientific
2. Others 2. The bearing and the locking service.
screw for the decantation coil
are defective.
7 The cell washer intensely vibrates. 1. Imbalance operation. 1. Balance correctly.
2. The bowl, rotor and distributor 2. Set the bowl, rotor and distributor
are not set correctly. correctly.
3. The center packing is not 3. Mount the center packing correctly.
mounted correctly. 4. Contact Thermo Fisher Scientific
4. Others. service.
8 A test tube breaks. 1. The test tube is not strong. 1. Use test tubes strong enough to
2. The test tube is different in size. bear the centrifugal force.

3. The rotor holder is deformed. 2. Use applicable-sized test tubes.

3. Contact Thermo Fisher Scientific
service.
9 Hemolysis is found in the blood cells. 1. Density of saline is improper. 1. Exchange for saline of proper
2. Sample is contaminated by density (0.9%).
bacteria. 2. Clean the tank, tube and pump
3. Fragments of a test tube are (especially the inside of the tank).
mixed. 3. Remove glass fragments in the
chamber and the drain cover.

Thermo Scientific CW3 | 41

Troublshooting when no Error Code is indicated

Decontamination Information
Certificate
INSTRUCTIONS
When an instrument used with radioactive, pathogenic, or otherwise hazardous materials requires servicing by Thermo
Fisher Scientific personnel either at the customer’s laboratory or at Thermo Fisher Scientific facilities, the following proce-
dure must be complied with to insure safety of our personnel:
1. The instrument or part to be serviced shall be cleaned of all blood and other encrusted material and decontaminated
prior to servicing by our representative.
No radioactivity shall be detectable by survey equipment.
2. A Decontamination Information Certificate shall be completed and attached to the instrument or part.
If an instrument or part to be serviced does not have a Decontamination Information Certificate attached to it, and, in our
opinion, presents a potential radioactive or biological hazard, our representative will not service the equipment until proper
decontamination and certification has been completed.
If an instrument is received at our service facilities and, in our opinion, poses a radioactive or biological hazard, the sender
will be contacted for instructions as the equipment is to be disposed. Disposition costs will be borne by the sender.
Additional certificates are available from your local technical or customer service representative. In the event these certif-
icates are not available, a written statement certifying that the instrument or part has been correctly decontaminated and
outlining the procedures used will be acceptable.
NOTE Thermo Fisher Scientific Service representatives will indicate on a Customer Service Repair Report if
decontamination was required, and if so, what the contaminate was and what procedure was used. If no decontamination
was required, it should be stated.

Decontamination Information Certificate

Complete and attach to equipment BEFORE servicing.

Decontamination
Certified By Title/Position
Phone FAX Department
Institution Address
City State ZIP
Instrument Serial Number
Rotor Serial Number
Part Part Number
Hazardous Contaminant(s) Decontamination Date
Decontamination Method(s)
Decontamination
Certifier‘s Signature: Date:

42 | CW3 Thermo Scientific

 Troublshooting when no Error Code is indicated

Chemical Compatibility

PET¹, Polyclear™,Clear Crimp™

Composite Carbon Fiber/Epoxy
MATERIAL

Anodic Coating for Aluminum

Polyester, Glass Thermoset

Cellulose Acetate Butyrate

Polyurethane Rotor Paint

Rulon A™, Teflon™

Ethylene Propylene

Polyvynil Chloride

Silicone Rubber

Stainless Steel
Polycarbonate

Polypropylene
Polythermide

Polyethylene
Polyallomer

Polysulfone
ALluminum

Neoprene
Delrin™

Titanium

Tygon™
Noryl™
Buna N

Viton™
Nylon
Glass
CHEMICAL
2-mercaptoethanol S S U / S M S / S U S S U S S / S S S S U S S S S S S

Acetaldehyde S / U U / / / M / U / / / M U U U M M / M S U / S / U

Acetone M S U U S U M S S U U S U S U U U S S U U S M M S U U

Acetonitrile S S U / S M S / S S U S U M U U / S M U U S S S S U U

Alconox™ U U S / S S S / S S S S S S M S S S S S S S S S S S U

Allyl Alcohol / / / U / / S / / / / S / S S M S S S / M S / / S / /

Aluminum Chloride U U S S S S U S S S S M S S S S / S S S S S M U U S S

Formic Acid (100%) / S M U / / U / / / / U / S M U U S S / U S / U S / U

Ammonium Acetate S S U / S S S / S S S S S S S U / S S S S S S S S S S

Ammonium Carbonate M S U S S S S S S S S S S S U U / S S S S S S M S S S

Ammonium Hydroxide (10%) U U S U S S M S S S S S / S U M S S S S S S S S S M S

Ammonium Hydroxide (28%) U U S U S U M S S S S S U S U M S S S S S S S S S M S

Ammonium Hydroxide (conc.) U U U U S U M S / S / S U S U U S S S / M S S S S / U

Ammonium Phosphate U / S / S S S S S S S S / S S M / S S S S S S M S S S

Ammonium Sulfate U M S / S S U S S S S S S S S S / S S S S S S U S S U

Amyl Alcohol S / M U / / S S / M / S / M S S S S M / / / U / S / M

Aniline S S U U S U S M S U U U U U U U / S M U U S S S S U S

Sodium Hydroxide (<1%) U / M S S S / / S M S S / S M M S S S S S S M S S / U

Sodium Hydroxide (10%) U / M U / / U / M M S S U S U U S S S S S S M S S / U

Barium Salts M U S / S S S S S S S S S S S M / S S S S S S M S S S

Benzene S S U U S U M U S U U S U U U M U M U U U S U U S U S

Benzyl Alcohol S / U U / / M M / M / S U U U U U U U / M S M / S / S

Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S

Cesium Acetate M / S / S S S / S S S S / S S / / S S S S S S M S S S

Cesium Bromide M S S / S S S / S S S S S S S / / S S S S S S M S S S

Cesium Chloride M S S U S S S / S S S S S S S / / S S S S S S M S S S

Cesium Formate M S S / S S S / S S S S S S S / / S S S S S S M S S S

Cesium Iodide M S S / S S S / S S S S S S S / / S S S S S S M S S S

Cesium Sulfate M S S / S S S / S S S S S S S / / S S S S S S M S S S

Chloroform U U U U S S M U S U U M U M U U U M M U U S U U U M S

Chromic Acid (10%) U / U U S U U / S S S U S S M U M S S U M S M U S S S

Chromic Acid (50%) U / U U / U U / / / S U U S M U M S S U M S / U M / S

Cresol Mixture S S U / / / S / S U U U U U U / / U U / U S S S S U S

Cyclohexane S S S / S S S U S U S S U U U M S M U M M S U M M U S

Deoxycholate S S S / S S S / S S S S S S S / / S S S S S S S S S S

Distilled Water S S S S S S S S S S S S S S S S S S S S S S S S S S S

Dextran M S S S S S S / S S S S S S S S S S S S S S S M S S S

Diethyl Ether S S U U S S S U S U U S U U U U U U U U U S S S S M U

Diethyl Ketone S / U U / / M / S U / S / M U U U M M / U S / / S U U

Diethylpyro-carbonate S S U / S S S / S S U S U S U / / S S S M S S S S S S

Dimethylsulfoxide S S U U S S S / S U S S U S U U / S S U U S S S S U U

Dioxane M S U U S S M M S U U S U M U U / M M M U S S S S U U

Ferric Chloride U U S / / / M S / M / S / S / / / S S / / / M U S / S

Acetic Acid (Glacial) S S U U S S U M S U S U U U U U M S U M U S U U S / U

Acetic Acid (5%) S S M S S S M S S S S S M S S S S S S S M S S M S S M

Acetic Acid (60%) S S U U S S U / S M S U U M U S M S M S M S M U S M U

Ethyl Acetate M M U U S S M M S S U S U M U U / S S U U S M M S U U

Ethyl Alcohol (50%) S S S S S S M S S S S S U S U S S S S S S S S M S M U

Ethyl Alcohol (95%) S S S U S S M S S S S S U S U / S S S M S S S U S M U

Ethylene Dichloride S / U U / / S M / U U S U U U U U U U / U S U / S / S

Ethylene Glycol S S S S S S S S S S S S / S U S S S S S S S S M S M S

Thermo Scientific CW3 | 43

Troublshooting when no Error Code is indicated

PET¹, Polyclear™,Clear Crimp™

Composite Carbon Fiber/Epoxy
MATERIAL

Anodic Coating for Aluminum

Polyester, Glass Thermoset

Cellulose Acetate Butyrate

Polyurethane Rotor Paint

Rulon A™, Teflon™

Ethylene Propylene

Polyvynil Chloride

Silicone Rubber

Stainless Steel
Polycarbonate

Polypropylene
Polythermide

Polyethylene
Polyallomer

Polysulfone
ALluminum

Neoprene
Delrin™

Titanium

Tygon™
Noryl™
Buna N

Viton™
Nylon
Glass
CHEMICAL
Ethylene Oxide Vapor S / U / / U / / S U / S / S M / / S S S U S U S S S U

Ficoll-Hypaque™ M S S / S S S / S S S S / S S / S S S S S S S M S S S

Hydrofluoric Acid (10%) U U U M / / U / / U U S / S M U S S S S M S U U U / /

Hydrofluoric Acid (50%) U U U U / / U / / U U U U S U U U S S M M S U U U / M

Hydrochloric Acid (conc.) U U U U / U U M / U M U U M U U U / S / U S U U U / /

Formaldehyde (40%) M M M S S S S M S S S S M S S S U S S M S S S M S M U

Glutaraldehyde S S S S / / S / S S S S S S S / / S S S / / S S S / /

Glycerol M S S / S S S S S S S S S S S S / S S S S S S S S S S

Guanidine Hydrochloride U U S / S S S / S S S S S S S / / S S S S S S U S S S

Haemo-Sol™ S S S / / / S / S S S S S S S / / S S S S S S S S S S

Hexane S S S / S S S / S S U S U M U S S U S S M S U S S U S

Isobutyl Alcohol / / M U / / S S / U / S U S S M S S S / S S S / S / S

Isopropyl Alcohol M M M U S S S S S U S S U S U M S S S S S S S M M M S

Iodoacetic Acid S S M / S S S / S M S S M S S / M S S S S S M S S M M

Potassium Bromide U S S / S S S / S S S S S S S S S S S / S S S M S S S

Potassium Carbonate M U S S S S S / S S S S S S U S S S S S S S S S S S S

Potassium Chloride U S S / S S S S S S S S S S S / S S S S S S S U S S S

Potassium Hydroxide (5%) U U S S S S M / S S S S / S U S S S S S S S M U M S U

Potassium Hydroxide (conc.) U U M U / / M / M S S / U M U U U S M / M U / U U / U

Potassium Permanganate S S S / S S S / S S S U S S S M / S M S U S S M S U S

Calcium Chloride M U S S S S S S S S S S S S M S / S S S S S S M S S S

Calcium Hypochlorite M / U / S M M S / M / S / S M S / S S S M S M U S / S

Kerosene S S S / S S S U S M U S U M M S / M M M S S U S S U S

Sodium Chloride (10%) S / S S S S S S / / / S S S S S / S S S S / S S M / S

Sodium Chloride (sat'd) U / S U S S S / / / / S S S S S / S S / S / S S M / S

Carbon Tetrachloride U U M S S U M U S U U S U M U S S M M S M M M M U S S

Aqua Regia U / U U / / U / / / / / U U U U U U U / / / / / S / M

Solution 555 (20%) S S S / / / S / S S S S S S S / / S S S / S S S S S S

Magnesium Chloride M S S / S S S S S S S S S S S S S S S S S S S M S S S

Mercaptoacetic Acid U S U / S M S / S M S U U U U / S U U S M S U S S S S

Methyl Alcohol S S S U S S M S S S S S U S U M S S S S S S S M S M U

Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U

Methyl Ethyl Ketone S S U U S S M S S U U S U S U U U S S U U S S S S U U

Metrizamide™ M S S / S S S / S S S S / S S / / S S S S S S M S S S

Lactic Acid (100%) / / S / / / / / / M S U / S S S M S S / M S M S S / S

Lactic Acid (20%) / / S S / / / / / M S M / S S S S S S S M S M S S / S

N/Butyl Alcohol S / S U / / S / / S M / U S M S S S S M M S M / S / S

N/Butyl Phthalate S S U / S S S / S U U S U U U M / U U S U S M M S U S

N, N-Dimethylformamide S S S U S M S / S S U S U S U U / S S U U S M S S S U

Sodium Borate M S S S S S S S S S S U S S S S / S S S S S S M S S S

Sodium Bromide U S S / S S S / S S S S S S S S / S S S S S S M S S S

Sodium Carbonate (2%) M U S S S S S S S S S S S S U S S S S S S S S S S S S

Sodium Dodecyl Sulfate S S S / S S S / S S S S S S S / S S S S S S S S S S S

Sodium Hypochlorite (5%) U U M S S M U S S M S S S M S S S S M S S S M U S M S

Sodium Iodide M S S / S S S / S S S S S S S / / S S S S S S M S S S

Sodium Nitrate S S S / S S S S S S S S S S S S / S S S S S U S S S S

Sodium Sulfate U S S / S S S S S S S S S S S S S S S S S S S M S S S

Sodium Sulfide S / S S / / / S / / / S S S U U / / S / / / S S M / S

Sodium Sulfite S S S / S S S S M S S S S S S M / S S S S S S S S S S

Nickel Salts U S S S S S / S S S / / S S S S / S S S S S S M S S S

Oils (Petroleum) S S S / / / S U S S S S U U M S M U U S S S U S S S S

Oils (Other) S / S / / / S M S S S S U S S S S U S S S S / S S M S

Oleic Acid S / U S S S U U S U S S M S S S S S S S S S M U S M M

Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S

44 | CW3 Thermo Scientific

 Troublshooting when no Error Code is indicated

PET¹, Polyclear™,Clear Crimp™

Composite Carbon Fiber/Epoxy
MATERIAL

Anodic Coating for Aluminum

Polyester, Glass Thermoset

Cellulose Acetate Butyrate

Polyurethane Rotor Paint

Rulon A™, Teflon™

Ethylene Propylene

Polyvynil Chloride

Silicone Rubber

Stainless Steel
Polycarbonate

Polypropylene
Polythermide

Polyethylene
Polyallomer

Polysulfone
ALluminum

Neoprene
Delrin™

Titanium

Tygon™
Noryl™
Buna N

Viton™
Nylon
Glass
CHEMICAL
Perchloric Acid (10%) U / U / S U U / S M M / / M U M S M M / M S U / S / S

Perchloric Acid (70%) U U U / / U U / S U M U U M U U U M M U M S U U S U S

Phenol (5%) U S U / S M M / S U M U U S U M S M S U U S U M M M S

Phenol (50%) U S U / S U M / S U M U U U U U S U M U U S U U U M S

Phosphoric Acid (10%) U U M S S S U S S S S U / S S S S S S S S S U M U S S

Phosphoric Acid (conc.) U U M M / / U S / M S U U M M S S S M S M S U M U / S

Physiologic Media (Serum, Urine) M S S S / / S / S S S S S S S S S S S S S S S S S S S

Picric Acid S S U / S M S S S M S U S S S U S S S S U S U M S M S

Pyridine (50%) U S U U S U U / U S S U U M U U / U S M U S S U U U U

Rubidium Bromide M S S / S S S / S S S S S S S / / S S S S S S M S S S

Rubidium Chloride M S S / S S S / S S S S S S S / / S S S S S S M S S S

Sucrose M S S / S S S S S S S S S S S S S S S S S S S S S S S

Sucrose, Alkaline M S S / S S S / S S S S S S U S S S S S S S S M S S S

Sulfosalicylic Acid U U S S S S S / S S S U S S S / S S S / S S S U S S S

Nitric Acid (10%) U S U S S U U / S U S U / S S S S S S S S S M S S S S

Nitric Acid (50%) U S U M S U U / S U S U U M M U M M M S S S U S S M S

Nitric Acid (95%) U / U U / U U / / U U U U M U U U U M U U S U S S / S

Hydrochloric Acid (10%) U U M S S S U / S S S U U S U S S S S S S S S U M S S

Hydrochloric Acid (50%) U U U U S U U / S M S U U M U U S S S S M S M U U M M

Sulfuric Acid (10%) M U U S S U U / S S M U S S S S S S S S S S U U U S S

Sulfuric Acid (50%) M U U U S U U / S S M U U S U U M S S S S S U U U M S

Sulfuric Acid (conc.) M U U U / U U M / / M U U S U U U M S U M S U U U / S

Stearic Acid S / S / / / S M S S S S / S S S S S S S S S M M S S S

Tetrahydrofuran S S U U S U U M S U U S U U U / M U U U U S U S S U U

Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M

Trichloroacetic Acid U U U / S S U M S U S U U S M / M S S U U S U U U M U

Trichloroethane S / U / / / M U / U / S U U U U U U U U U S U / S / S

Trichloroethylene / / U U / / / U / U / S U U U U U U U U U S U / U / S

Trisodium Phosphate / / / S / / M / / / / / / S / / S S S / / S / / S / S

Tris Buffer (neutral pH) U S S S S S S / S S S S S S S S S S S S S S S S S S S

Triton X/100™ S S S / S S S / S S S S S S S S S S S S S S S S S S S

Urea S / U S S S S / / / / S S S M S S S S / S S S M S / S

Hydrogen Peroxide (10%) U U M S S U U / S S S U S S S M U S S S S S S M S U S

Hydrogen Peroxide (3%) S M S S S / S / S S S S S S S S M S S S S S S S S S S

Xylene S S U S S S M U S U U U U U U M U M U U U S U M S U S

Zinc Chloride U U S S S S U S S S S S S S S S S S S S S S S U S S S

Zinc Sulfate U S S / S S S S S S S S S S S S S S S S S S S S S S S

Citric Acid (10%) M S S M S S M S S S S S S S S S M S S S S S S S S S S

¹
Polyethlyeneterephtalate

S – Satisfactory.

M – Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of

use.

U – Unsatisfactory, not recommended.

/ – Performance unknown; suggest testing, using sample to avoid loss of valuable material.

NOTI C E
Chemical resistance data is included only as a guide to product use. Because no organized chemical compatibility
data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots.

Thermo Scientific CW3 | 45

Troublshooting when no Error Code is indicated

Index O
Operating Conditions 31
Operation 29
A Operation Modes 33

Agitation-Centrifugation 33
Antiglobulin Test 34 P
Automatic Mode 33
Precautions 7
Preface 5
B Preparation 29
Preparation of the Sample 32
Before Setting Up 19 Pump 36
Bowl 36 Pump Ventilation 29

C R
Chamber 36 Rotor 36
Chemical Compatibility 43
Condition-setting Panel 17
Control Panel 16 S
Setting Up 20
D Set Up 19
Shipping 28, 38
Decontamination Information Certificate 42 Splash Guard 36, 37
Directives and Standards 13 Storage 28
Disposal 38 Symbols Used in the Manual 11
Distributor 36 Symbols Used on the Cell Washer 10
Door Stopper 37
Door Stopper Parts 36
Drain Cover 36, 37 T
Tank 36
E Technical Specifications 12
Transport 19
Error Codes 40 Transporting 19
Troubleshooting 39
I Troublshooting 41
Tubing 36
Injection Volume Adjustment 29
Intended Use 7
Items Supplied 5

L
Leveling 20
Location 19
Location and Function of Parts 14

M
Mains Connection 20
Mains Supply 13
Maintenance 35
Manual Mode 33
Mechanical Emergency Door Release 39

46 | CW3 Thermo Scientific

Thermo Electron LED GmbH
Zweigniederlassung Osterode
Am Kalkberg, 37520 Osterode
Germany

thermoscientific.com/centrifuge
© 2016 Thermo Fisher Scientific Inc. All rights reserved.

Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of
Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain Performance
Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered trademark of Haemo-Sol.
Triton X-100 is a registered trademark of Sigma-Aldrich Co. LLC. Valox is a registered trademark of General Electric Co.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.
Shown pictures within the manual are examples and may differ considering the set parameters and language.

United States/Canada +1 866 984 3766 Netherlands +31 76 579 55 55 China +800 810 5118 or
Latin America +1 866 984 3766 Nordic/Baltic States/CIS +358 9 329 10200 +400 650 5118
Austria +43 1 801 40 0 Russia +7 812 703 42 15 Japan +81 3 5826 1616
Belgium +32 53 73 42 41 Spain/Portugal +34 93 223 09 18 Other Asian Countries +852 2885 4613
France +33 2 2803 2180 Switzerland +41 44 454 12 22 Australia +61 39757 4300
Germany national toll free 0800 1 536 376 UK/Ireland +44 870 609 9203 New Zealand +64 9 980 6700
Germany international +49 61 84 90 6000 India +91 22 6716 2200 Countries not listed +49 6184 90 6000 or
Italy +39 02 95059 552 +33 2 2803 2180

50144259 original instruction manual published in 11 / 2016.