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Procedure Procedure: A. Make Gut Extract: A. Make Gut Extract

This document outlines procedures for extracting and testing gut enzymes. The procedures involve: 1) extracting gut enzymes from intestines by surgically removing the intestines, cleaning them, and grinding them in a solution over several days; 2) testing the bile extract's ability to break down fat using a cooking oil experiment; 3) testing for the presence of the enzyme amylase using a starch solution that should change color in the presence of amylase; 4) similarly testing for the presence of the enzyme maltase using a maltose solution; and 5) testing for the presence of the enzyme trypsin using an egg white solution that should change color with the biuret reagent in the presence of trypsin.

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11 views5 pages

Procedure Procedure: A. Make Gut Extract: A. Make Gut Extract

This document outlines procedures for extracting and testing gut enzymes. The procedures involve: 1) extracting gut enzymes from intestines by surgically removing the intestines, cleaning them, and grinding them in a solution over several days; 2) testing the bile extract's ability to break down fat using a cooking oil experiment; 3) testing for the presence of the enzyme amylase using a starch solution that should change color in the presence of amylase; 4) similarly testing for the presence of the enzyme maltase using a maltose solution; and 5) testing for the presence of the enzyme trypsin using an egg white solution that should change color with the biuret reagent in the presence of trypsin.

Uploaded by

Muthiaranifs
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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PROCEDURE

a.
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Make gut extract:


Surgical carp on the stomach.
Separate the gut of other organs carefully. Take the small intestine
by cutting from the latter part of the stomach to the beginning of
the large intestine.
Take the bile bag carefully and not to break
Open small intestine by means of longitudinal slashed.
Clean the small intestine of the premises of distilled water, and
then insert it into the mortar.
Take 20 ml of 50% glycerol and insert it into the mortar, and puree
intestines. Take 4-5 drops toulen, puree back. After the intestinal
smooth enter into the bottle, then close tightly. Wrap the bottle with
carbon paper.
Save the gut extract in the dark for 6-7 days.
After 6-7 days, strain the gut extract with filter paper.
Perform the test on the filtered solution is proving tests amylase,
maltase, and trypsin.

b. Test effect of bile for fat:


1. Prepare 2 Test Tube,
2. Give Label A And B
3. Take in bile into test tube a with little cut in surface
4. Dilute the bile with aquades become 20 ml
5. Take in 20 ml aquades into test tube b as control
6. Add to each test tube 2 ml cooking oil
7. Shake it and allow it to 5-10 minutes
8. Observe what happened at 2 substance that in test tube
9. Compare how big the clots of fat in each test tube

c. Tests proving the existence of amylase:


1. Provide two test tubes and labeled A and B. Pour benedict reagent
into the tube 2 ml respectively.
2. Prepare sua another tube and labeled C and D.
3. Enter cooked starch solution dilute each 2 ml into the tube C and D.
For C tube add 1 ml of extract specific whereas for D tube add 1 ml of
distilled water. Shake the tube stretcher for 5-10 minutes.
4. Apply a drop of as much as 5 drops of the solution in the tube C to
tube A, and the solution in the tube D to tube B.
5. Heat the tubes A and B for 5 minutes and observe the color change
that occurs in the tube solution A and B.

d. Tests proving the existence of maltase:


1. Provide two test tubes and labeled A and B. Pour benedict reagent into
the tube 2 ml respectively.
2. Prepare sua another tube and labeled C and D.
3. Enter maltose dilute each 2 ml into the tube C and D. For C tube add 1
ml of extract specific whereas for D tube add 1 ml of distilled water.
Shake the tube stretcher for 5-10 minutes.
4. Apply a drop of as much as 5 drops of the solution in the tube C to tube
A, and the solution in the tube D to tube B.
5. Heat the tubes A and B for 5 minutes and observe the color change
that occurs in the tube solution A and B.

e. Test proving tripsin


1. Prepare 2 test tube
2. Give the label a and b
3. Add to a and b 1 ml white egg dilute
4. Burn in after boiling
5. Chill its tube
6. After it add the extract of intestine to test tube a 1 ml
7. Add 1 ml aquades into test tube b
8. Chill it 5-10 minutes
9. Drop reagen biuret 5 drops to test tube a
10. Observe the change of color which happened in test tube

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