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Food Tests - Year 10

This document outlines the procedures for common food tests to identify macromolecules like carbohydrates, proteins, and fats. It describes using iodine to test for starch (turns black), Benedict's solution to test for sugars (turns orange), Biuret's reagent to test for proteins (turns purple), and Sudan III to test for fats (forms a red-stained oil layer). The purpose is to teach students how to perform basic nutrient identification tests in a practical lab setting.
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0% found this document useful (0 votes)
215 views17 pages

Food Tests - Year 10

This document outlines the procedures for common food tests to identify macromolecules like carbohydrates, proteins, and fats. It describes using iodine to test for starch (turns black), Benedict's solution to test for sugars (turns orange), Biuret's reagent to test for proteins (turns purple), and Sudan III to test for fats (forms a red-stained oil layer). The purpose is to teach students how to perform basic nutrient identification tests in a practical lab setting.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
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Food test required practical

Starter:
Fill in table
Food What foods you find it in Its role in the body
group

Ext: what is the difference between an organ and


a tissue?
Positive foods Negative foods

Starch—
Iodine

Glucose
(sugar)—
Benedict’s
solution
Protein—
Biuret test

Fats—
Sudan III
Practical time!
Testing for glucose

Blue Benedict’s solution turns orange/red when it is heated


to 70C with glucose.
Benedict’s
solution

orange/red
chopped hot water = glucose
up food

heat
Orange/red = glucose
Testing for starch

Orangey-brown iodine turns blue-black when it reacts with


starch.

Iodine

drop iodine
solution onto the
food

black = starch
Testing for protein

In the Biuret test, the solution turns purple when it reacts


with protein.

Biuret’s
reagent
shake

chopped up
food

purple = protein
Testing for fats

Sudan III solution is used to test fats.

Transfer
grind food into a beaker
And add water
Half fill the test tube
with the solution
add 3 drops of
Sudan III and shake.

Red stained oil layer=fat


Positive foods Negative foods

Starch—
Iodine

Glucose
(sugar)—
Benedict’s
solution
Protein—
Biuret test

Fats—
Sudan III
Aim: Test for the presence of different nutrients in
foods.

HoM: Personal organisation.

Test Nutrient Starting Positive


chemical colour colour change
Iodine

Benedict’s
solution
Biuret’s
solution
Ethanol &
water
Aim: Test for the presence of different nutrients in
foods.

HoM: Personal organisation.

Test Nutrient Starting Positive


chemical colour colour change
Iodine Starch Brown Blue/Black

Benedict’s Glucose Blue Orange


solution
Biuret’s
Protein Blue Purple
solution
Ethanol & Fats Clear Milky white
water emulsion
On whiteboards

What chemical do you use in


the food test for starch?
On whiteboards

What chemical do you use in


the food test for fats?
On whiteboards

What chemical do you use in


the food test for protein?
On whiteboards

What chemical do you use in


the food test for sugar?
Exam question - digestion

Describe the food tests used to identify food


groups. You need to include the test for protein,
lipids and different carbohydrates. You need to
state which reagent is used and the positive result
for each food group (8 marks).
Answer - digestion
Plenary SPLAT

STOMACH GALL BLADDER

IODINE BLACK

LIPID INTESTINE

ORGAN TISSUE

BENEDICTS REAGENT PANCREAS

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