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Semen Collection Methods

This presentation includes modern techniques of semen collection in animals. It is of Veterinary importance

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Nabeel
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0% found this document useful (0 votes)
49 views28 pages

Semen Collection Methods

This presentation includes modern techniques of semen collection in animals. It is of Veterinary importance

Uploaded by

Nabeel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
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Semen Collection Methods

Preparation of AV
• Semen is an organic fluid, also known as
seminal fluid, that usually contains spermatozoa.
• It is secreted by the gonads.
• Seminal fluid contains several components
besides spermatozoa: proteolytic and other
enzymes as well as fructose are elements of
seminal fluid which promote the survival of
spermatozoa and provide a medium through
which they can move or "swim."
Sperm Pathway Out of Body
Seminiferous tubules

Rete Testis

Efferent ducts

Caput Epididymus

Corpus Epididymus

Cauda Epididymus

Ductus deferens

Ampulla

Pelvic Urethra

Penile Urethra
Objective of semen collection

a. Maximum use of superior sires

b. Evaluate if male is sterile or fertile


Methods of Semen Collection
• Recovery
• Massage
• Artificial vagina
• Digital manipulation
• Electro-ejaculation
Recovery
• Taking semen from vagina with
spoon/siphon after breeding

• By placing the rubber breeding


bag/sponge in vagina before copulation

Recovered semen is of poor quality


Massage Method
In bulls
•By inserting hand in the rectum
•Pressure exerted in milking motion on the
ampulla, prostate and vesicular gland
•Semen drips without erection and extension
•Semen is of poor quality & contaminated with
urine and debris
In Poultry
•only practical solution to get ejaculation
Artificial Vagina Methods
• Artificial vagina (AV) is used to collect
semen from cattle and horses and also
sheep and goats
Components of AV

• Rigid rubber casing with an opening at 10 cm


from one end
• Rough-textured latex inner liner
• Rubber band
• Rubber funnel
• Graduated glass collection tube
• Insulated and zippered jacket during cold
season
Different shape and size by species
AV
AV’s for Cattle
AV’s for sheep
AVs for Stallion
Requirements of AV

• Pressure: By filling water and a little air


into the space between casing and inner
liner
• Temperature: By warm water of 39 °C at
collection (temperature of water at
filling:43-46 °C)
• Lubrication: Around front area of liner with
sterile and harmless lubricating jelly (KY
Jelly)
Collecting from the bull

• AV should be held parallel to the expected path


of bull's penis, after false mounts
• Guide penis to the side by grasping sheath and
direct it into AV
• Bull will thrust and ejaculate simultaneously
Precautions:
a. Prevent cold shock
b. Protect collection tube from sunlight
c. Avoid contamination of semen by urine, water
or lubricating jelly
d. Use a different sterile AV for each ejaculation
Advantages of AV method
Disadvantages of AV Method
Electro-ejaculation
Principle:
• Electrically stimulating nerves of male reproductive system
Uses for bulls:
• For crippled, aged or sexually low active dairy bulls
• For checking semen quality of beef bulls prior to natural
breeding season
Equipment:
• A bipolar electrode and a variable source of alternating
current: voltage: 0 - 30 V, amperage: 0.5 - 1.0 A;
alternating + and - rings spaced 4cm apart, running
longitudinally along the electrode
• Semen quality as compared with AV ejaculates: larger
volume, lower in sperm concentration, sometimes semen
contamination by urine
Power
control

Probe

Collecting
tube handle
Electro-ejaculation procedure
Advantages of Electro-
ejaculation
• Semen can be collected from males that
are too young or old or unable to mount
due too weak or injured legs.
• No female or dummy is required for
collection.
• Less chance of contamination.
Disadvantages of Electro-
ejaculation
• Highly technical method and need
considerable skill and practice.
• The semen generally gets contaminated
with urine.
• Some males resist too much to this
method and refuse collection.
• Sciatic nerves are temporarily affected
during the operation but is relatively minor
if the electrodes are kept over the ampullar
region.
Digital Manipulation

• By applying pressure and massage to the


penis
• Cone attached to a collection tube, is
slipped over the erected penis to facilitate
harvesting the semen
• Used in dogs
• Semen is collected from chickens and
turkeys using a form of digital manipulation
The Ejaculate
Spec. Volume Conc. Color
(ml) (# cells/ml)
Bull 5 - 15 800 M - 1 B Creamy
white
Ram .8 -1.2 2-3B Creamy
and thick
Boar 150 - 200 200 - 300 M Milky
Stallion 60 - 100 200 - 500 M Grayish
white
Rooster .2 - 1.5 500 - 800 M White
thik

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