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Scanning Electron Microscope: Andhra Mahila Sabha Arts and Science Amberpet

The document provides an overview of Scanning Electron Microscopes (SEM), detailing their principles, components, and working mechanisms. It highlights the importance of sample preparation, types of signals generated, and various applications in fields such as material science, biology, and forensics. Additionally, it discusses the advantages and limitations of SEM technology.

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Tejashwini Poshamoni
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4 views11 pages

Scanning Electron Microscope: Andhra Mahila Sabha Arts and Science Amberpet

The document provides an overview of Scanning Electron Microscopes (SEM), detailing their principles, components, and working mechanisms. It highlights the importance of sample preparation, types of signals generated, and various applications in fields such as material science, biology, and forensics. Additionally, it discusses the advantages and limitations of SEM technology.

Uploaded by

Tejashwini Poshamoni
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Scanning Electron Microscope

ANDHRA MAHILA SABHA ARTS AND SCIENCE


AMBERPET
DEPARMENT:ORGANIC CHEMISTRY

Submitted by
A. Shravani
105824503015
Introduction

 SEM is a type of electron


microscope.
 Uses a focused electron beam
to produce high-resolution
surface images.
 Developed in the 1930s,
widely used since the 1960s.
Principle of SEM

 SEM uses electrons instead of light.


 Electron beam interacts with atoms in the
sample.
 Signals from interactions form detailed
images.
Parts of SEM

 Detectors (SE, BSE, etc.)


 Electron gun
 Electromagnetic lenses
 Scanning coils
 Sample chamber
 Display/Monitor system
Working of SEM

 Electron gun emits a beam of


electrons.
 Beam is focused by
electromagnetic lenses.
 Beam scans the surface of the
sample.
 Interaction signals are collected
to form an image.
Sample Preparation

 Samples must be dry and conductive.


 Non-conductive samples are coated with gold or carbon.
 Mounted on stubs using conductive adhesives.
Types of Signals and Images

 Secondary Electrons (SE):


Surface topography
 Backscattered Electrons
(BSE): Composition
contrast
 X-rays: Elemental
analysis (EDS/EDX)
Applications

 Material science: Fracture analysis, metallurgy


 Biology: Cell surface imaging
 Forensics: Gunshot residue, trace evidence
 Electronics: Microchip inspection
 Nanotechnology: Nanoparticles
Advantages and Limitations

Advantages:
 High resolution
 3D-like imaging
 Large depth of field
Limitations:
 Expensive
 Requires vacuum
 Samples must be conductive
Conclusion

 SEM is crucial in scientific and industrial


research.
 Enables high-resolution surface imaging and
analysis.
 Widely applied in multiple domains for
precise visualization.

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