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Water Lab Report - E.coli (FINAL)

Water Supply E.Coli in water Lab Report Detection of E.Coli In water sample The presence of Micoorganism in water

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4K views6 pages

Water Lab Report - E.coli (FINAL)

Water Supply E.Coli in water Lab Report Detection of E.Coli In water sample The presence of Micoorganism in water

Uploaded by

SP Aslam Syzn
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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TITLE:

IDEXX ColilertTest Method For The Simultaneous Detection Of Total Coliform


and E. Coli In Water
OBJECTIVE:
To detect and confirm of total coliform and E. Coli in water.
INTRODUCTION:
IDEXX Colilert reagent is used around the world for the detection of coliforms
and E. coli in water. Colilert is used for the simultaneous detection, specific
identification, and confirmation of total coliforms and E. Coli in water. Colilert
based on the Defined Substrate Technology(DSTTM). DST utilizes indicator
nutrients which cause target microbes contained in the sample and
incubated in the DST reagent system to produce a colour change (or another
signal, ie: fluorescence), both indicating and confirming their presence. The
indicator-nutrient is cleaved by the target microbe which metabolizes the
nutrient and frees the indicator to express a specific color. Total coliform and
E. Coli are specifically and simultaneously detected and identified the 1
CFU/100ml of sample, in 24 hours or less, by inoculating the reagent with the
water sample and incubating it.
APPARATUS:
IDEXX Colilert Reagent
E. Coli
Paddy Water Sample

PROCEDURE:
1. Contents of one pack to a 100ml water sample was added to a sterile
vessel.
2. The vessel was cap and shake until dissolved.
3. The reagent mixture was poured into a Quanti tray and sealed in an
INDEXX Quanti Tray Sealer.
4. Next, the sealed tray was placed in a 35C incubator for 24 hours.
5. Then, the result was read according to the result interpretation table.
6. If no yellow color is observed, the test is negative.
7. If the sample has a yellow color equal to or greater than the comparator,
the presence of total coliforms is confirmed.
8. The number of positive wells was counted and MPN table provided was
referred.

RESULTS:
Number of box
Group

W1
W2
W3
W4
W5
W6

Type of

Large

Small

MPN of

Bacteria
Coliform

46

48

Bacteria
533.5

E. coli

30

58.8

Coliform

47

47

665.3

E. coli

27

15

60.8

Coliform

48

48

1011.2

E. coli

28

47.3

Coliform

47

48

691.0

E. coli

42

39

227.7

Coliform

47

48

691.0

E. coli

47

38

472.1

Coliform

47

48

691.0

E. coli

47

38

472.1

QUESTIONS:
MICROBIOLOGICAL ANALYSIS DETERMINATION OF E. COLI AND TOTAL
COLIFORM
1. What is the family name of coliform and E. Coli group?
Coliform bacteria and E. Coli are the type of bacteria that are part of the
enterobacteriaceae family.

2. What is the use of UV light? Why that bacteria must be observed under UV
light?
The use of UV light is to detect the presence of bacteria. Because
bacteria tend to congregate on surfaces where high levels of flavin are
present. Flavin is a material that emits a fluorescent glow when
exposed to UV light.

3. What to do if water sample has some background color? How to make the
dilution?
If there is color, you should examine again with more dilution. Dilute the
samples

well

with

PBS

of

0.9%

NaCl.

PBS

or

0.9%

NaCl

is

recommendable for the dilution. (If you must use water, apply 0.9 mL or
1.0 mL of sample solution and incubate at 35 or 30, respectively.)

DISCUSSION:
The

Colilert-18

system

for

enumeration

of

total

coliforms

and Escherichia coli is approved by the U.S. Environmental Protection Agency


for use in drinking water analysis and is also used by various agencies and
research studies for enumeration of indicator organisms in fresh and saline
waters.

Coliform

bacteria

are

widely

used

as

indicators

of

fecal

contamination of both fresh and marine waters. Certain members of the


coliform group live outside of the gastrointestinal tract in the environment
and may create a false indication of fecal contamination. Index colilert test
method is used for the simulteneous detection oftotal coliform and E.Coli in
water. From our experiment, MPN of bacteria of coliform in water sample 3 is
very higher than other 5 water sample. It is 1011.2. we can see the present
of coliform by look at the water sample that turn into yellow flourescent. MPN
of bacteria for water sample 1,2, is 533.5, 665.3 while water sample 4,5,6 is
in the same value. It is 691.0.For MPN of bacteria for E.coli is very higher in
water sample 5 and 6. The value is 472.1. other MPN of bacteria in water
sample 1,2,3 and 4 are 58.8, 60.8, 47.3, and 227.7. from this experiment, we
can conclude that the water sample that has higher number of MPN of
bacteria for E.coli is very polluted.

REFERENCES:
1. Avocet Environmental Testing. Total Coliform Bacteria Testing. Retrieved
April

15,

2013,

from

http://www.avocetlab.com/drinking-water/what-

mean/interpreting-results/

2. John Lindquist. (2009). Bacteriology 102. A General Overview of Coliforms.


Retrieved

April

15,

2013,

from

http://www.splammo.net/bact102/102coliforms.html

3. Ian L. Pepper, Charles P. Gerba. (2011). Experiment 17: Defined Substrate


Technology for Detection of Coliforms and Fecal Coliforms. Environmental
Microbiology: A Laboratory Manual (pp. 129-133). Academic Press.
Retrieved

April

15,

2013

from

http://books.google.com.my/books?

id=0cFbAoJ_c7UC&pg=PA132&dq=coliform+e.

+coli+colilert+test+experiment&hl=en&sa=X&ei=zK1rUZKmH4HIrQeji4G
wAQ&ved=0CC0Q6AEwAA#v=onepage&q=coliform%20e.%20coli
%20colilert%20test%20experiment&f=false

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