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GYC

This document summarizes the GYC AGAR medium used for detecting acid-producing microorganisms such as acetic acid bacteria. The medium contains dextrose, yeast extract, calcium carbonate, and bacteriological agar. Acetic acid bacteria will produce clear zones around colonies as the acid produced neutralizes the calcium carbonate. The medium supports growth of Acetobacter aceti as confirmed through testing of the type strain. The prepared medium should be stored at 2-8°C.

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3K views2 pages

GYC

This document summarizes the GYC AGAR medium used for detecting acid-producing microorganisms such as acetic acid bacteria. The medium contains dextrose, yeast extract, calcium carbonate, and bacteriological agar. Acetic acid bacteria will produce clear zones around colonies as the acid produced neutralizes the calcium carbonate. The medium supports growth of Acetobacter aceti as confirmed through testing of the type strain. The prepared medium should be stored at 2-8°C.

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GYC AGAR

CAT N: 2026 Acetic Bacteria Medium

FORMULA IN g/l
Dextrose 50.0 Calcium Carbonate 5.0

Yeast Extract 10.0 Bacteriological Agar 20.0

Final pH 6.8 0.2 at 25C

Acetobacter aceti
ATCC 15973

PREPARATION
Suspend 85 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. If desired, cool to 45-50C and aseptically add 70 ml of ethanol. AVOID
OVERHEATING. DO NOT AUTOCLAVE. Mix well and dispense into plates. The prepared medium should be stored at 2-
8C. The color of the prepared medium is light amber, slightly opalescent.

The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.

USES
GYC AGAR described by Swings (1992) detects the presence of acid-producing microorganisms and is regarded as
standard growth medium for acetic acid bacteria.

Dextrose is the fermentable carbohydrate providing carbon and energy. Yeast extract is source of vitamins, particularly
the B-group. Grown on this medium, Acetobacter will produce clear zones or halos around colonies because the acid
being produced will neutralize the CaCO3. Unlike the lactic acid bacteria, acetic acid bacteria are obligate aerobes and so
it is necessary to use spread plates. Bacteriological agar is the solidifying agent.

Inoculate and incubate at 28-30C for 3-5 days.

MICROBIOLOGICAL TEST
The following results were obtained from type cultures in the performance of the medium after incubation at a
temperature of 28-30C and observed after 3-5 days.

Microorganisms Growth
Acetobacter aceti ATCC 15973 Good

BIBLIOGRAPHY
Madigan M.Martinko J (editors) (2005). Brock Biology of Microorganisms (11th ed. Edicin).

LABORATORIOS CONDA, S.A. www.condalab.com


STORAGE 25C

Once opened keep powdered medium closed to avoid hydration.


2C

LABORATORIOS CONDA, S.A. www.condalab.com

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