3D Cell Culture Global Market

{Technology (Scaffold based platforms and Scaffold free

platforms); Products (Cells & Tissues, Media, Sera &
Reagents, Microplates & Others, Assay kits, Scaffolds and
Others)]; Applications (Basic Research, Toxicity & Drug Safety
2018
Screening, Tissue Engineering, Stem Cell Research, Drug
Discovery, Cancer Research and Gene Therapy); END-USERS
(Academic Institutes, Biotechnology & Pharmaceutical
Industries, and Others); Regional (North America, Europe,
Asia Pacific and Rest of the World)}

– Forecast to 2024

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LIST OF COMPANIES MENTIONED IN THE REPORT

Company Headquarters

300 Microns GmbH Germany

3D Biotek, LLC U.S.

3D Matrix Co. Japan

3Dtro AB Sweden

Advanced Biomatrix, Inc. U.S.

AIM Biotech PTE Ltd. Singapore

Akron Biotechnology, LLC U.S.

amsbio LLC U.K.

Ascendance U.S.

Aspect biosystems Canada

Asterand Bioscience U.S.

Avanticell Science U.K.

BASF Germany

Becton, Dickinson and Company U.S.

BellBrooks Labs U.S.

Bellco Glass, Inc U.S.

BioCellChallenge France

Biogelx Ltd. U.K.

Biolamina AB Sweden

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Company Headquarters

Biomerix U.S.

BioVision, Inc. U.S.

Biozol GmbH Germany

BISS TGT India

BRTI Life Sciences U.S.

Celartia U.S.

Cell Applications, Inc. U.S.

Cell bio labs U.S.

Cell spring Switzerland

CellecBiotek AG Switzerland

Cellendes GmbH Germany

Cellevate AB Sweden

Cellink Sweden

CELLnTEC Advanced Cell Systems AG Switzerland

Celprogen U.S.

CelVivo IVS Denmark

Charles River Laboratories U.S.

CNBio Innovations Ltd. U.K.

Corning Inc. U.S.

CTIBiotech France

Cyfuse Biomedical KK Japan

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Company Headquarters

Cytoo France

Dia Genode Belgium

DWK Life Sciences Germany

EBERS Medical Technology SL Spain

Ectica Technologies AG Switzerland

Electrospinning Company U.K.

Emulate, Inc. U.S.

ESI Bio U.S.

FiberCell Systems Inc. U.S.

Fluidinova SA Portugal

Fujifilm Japan

Genlantis (Gene Therapy Systems, Inc.) U.S.

Global Cell Solutions, Inc U.S.

Gradientech AB Sweden

Greiner Bio-One GmbH Austria

Guangzhou JET Bio-Filtration Co., Ltd. China

Keranetics U.S.

Hokkaido Soda Japan

Hurel Corp U.S.

ibidi GmbH Germany

Insphero Switzerland

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Company Headquarters

Invivo Sciences U.S.

Irvine Scientific U.S.

IV Tech Srl Italy

Japan Vilene Company Japan

JSR Corporation (Organogenix Inc.) Japan

Kirkstall Ltd U.K.

Koken Co. Ltd. Japan

Kolloidis Biosciences U.S.

Kuraray Co., Ltd. Japan

Lena Biosciences, Inc. U.S.

Lifecore Biomedical, U.S.

Locate Therapeutics U.K.

Lonza Group Switzerland

Matricel GmbH Germany

MatTek Corporation U.S.

Menicon Life Science Japan

Merck Kgaa Germany

Microtissues, Inc. U.S.

Miltenyi Biotec GmbH Germany

Mimetas B.V. Netherlands

Mirus Bio U.S.

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Company Headquarters

Nano 3D Biosciences Inc U.S.

Nanofiber Solutions U.S.

Nanolive SA Switzerland

Nanopharma a.s. Czech Republic

Neuromics Inc U.S.

Organovo Holdings, Inc U.S.

PepGel LLC U.S.

PerkinElmer U.K.

Pishon Biomedical Taiwan

Prodizen Korea

Promega Corporation U.S.

PromoCell GmbH Germany

Protista International AB Sweden

QGel Bio Switzerland

Quorus Biotech (Pty) Ltd. South Africa

RealBio Technology Inc. U.S.

Regenovo China

Reprocell (Reinnervate Ltd. ) U.K.

Sangi Co., Ltd. Japan

Sartorius Stedim Biotech U.K.

Spheritech Limited U.K.

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Company Headquarters

STEMCELL Technologies Inc. Canada

StemTek Spain

Sumitomo Bakelite Co. Ltd. Japan

Synthecon, Inc U.S.

SynVivo LLC U.S.

Takara Bio Inc (Clontech) Japan

Tetec AG (B Braun) Germany

The Well Bioscience Inc. U.S.

Thermo Fisher Scientific U.S.

Trevigen, Inc. U.S.

UPM Finland

Vornia Biomaterials Ltd. Ireland

Xanofi U.S.

zPREDICTA, Inc. U.S.

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1 EXECUTIVE SUMMARY

The first attempt to culture cell artificially dates back to 1907, where Dr. Ross Harrison, an
Anatomy Professor, developed hanging drop technique to grow three-dimensional (3D) frog
embryonic nerve fragments via a coverslip. Since then, life science researchers have
depended on primarily adherent, two-dimensional (2D) cell culture techniques where cells of
interest are cultured in plastic or glassware for study of human cells, tissues, and disease
models. As intricacies of these techniques are understood advanced 3D models have been
developed which greatly influence the in-vitro culturing and driving cell culture market. In
contrast to 2D environment, cells grown in three-dimensions display higher degree of
intercellular interactions, assume more physiologically relevant morphologies and preserve
higher-order tissue processes. Tools, technologies, and protocols are making in-vitro 3D
analysis simple, cheap, reproducible and scalable-enough for adaptation in drug
development. As these tools have become more accessible and validated, researchers in
both academia and biopharma are shifting their attention from 2D to 3D cell culture. As
estimated by IQ4I Research, the 3D cell culture global market is valued at $XX million in
2017 and is expected to grow at a low double digit CAGR to reach $1,485.1 million by 2024.

Rising investments in research and development, increasing need for organ transplantation,
use of 3D cell culture models as a tool against animal testing, use of microfluidic technology,
and increasing focus on regenerative medicine are some of the factors that are driving the
market growth. The entry of new players with novel technologies and introduction of
technology driven products are creating an opportunity in the global 3D cell culture market.
Whereas, Lack of experienced and skilled research professionals, lack of consistency in 3D
cell culture products and budget restriction for small and medium-sized laboratories are
hindering the market growth. Stringent regulatory process controls and ethical concern over
animal source usage are the major threat to the market.

The recent progress in microfluidic technology, scaffolds, spheroids and gels-based systems
is making lot of difference in the market as they provide micro-scale complex structures and
well-controlled parameters to mimic the in vivo environment of cells and thereby gives an
opportunity for scientists to explore new applications such as tissue engineering, stem cell,
drug discovery and regenerative medicine. The rapid advancements in the field of cell
culture such as 3D bioprinting, organ-on-chip, and single use bioreactors is advancing day-

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by-day and has been accepted as the future direction of 3D cell culture market. Now-a-days,
ultra low attachment micro-plates (ULA) applications are increasing as researchers are
focusing on single spheroid and microtissue production for downstream assay compatibility
driving demand for ultra low attachment microplates. For instance, InSphero AG
(Switzerland), Corning Incorporated (U.S.), Perkin Elmer (U.S) are some the major
companies manufacturing these plates.

The 3D cell culture technology global market is broadly classified into scaffold based
platforms and scaffold free platforms, of which the scaffold based platforms, hold the largest
share in 2017. However the scaffold free platforms are expected to grow at a faster rate.
Furthermore scaffolds hold the largest share among the scaffold based platforms that
include hydrogels and other products as well. Similarly, the forced floating method occupied
the largest share within the scaffold free market when compared to the other methods such
as hanging drop method, agitation based methods and microfluidics.

A number of product categories are available in the 3D cell culture market that include cells
& tissues, media, sera and reagents, microplates and others, assay kits, scaffolds and other
products. The media, sera and reagents segment occupy the largest share in 2017.
However the scaffolds market seems to be the fastest growing, due to the advancements in
technologies in this area including the uptake of bioprinting technology.

There are a number of applications in which 3D cell culture products are used that include
basic research, toxicity and drug safety screening, tissue engineering, stem cell research,
drug discovery, gene therapy and cancer research. The toxicity and drug safety screening is
a field in which recent advanced are recognized making it the largest segment in 2017. From
an end-users perspective the biotechnology and pharmaceutical industries occupy the
largest share in 2017 followed by CROs and hospitals is growing at the fast rate.

Geographically, 3D cell culture market is divided into North America, Europe, Asia-Pacific
and Rest of the world. North America dominated the market with largest share in 2017, due
to increased government funding, rising incidence of cancer, development of new models for
candidate drug screening and cytotoxicity testing, expanding biopharmaceutical industries
and high healthcare expenditure. Europe accounts for second largest share followed by
Asia-pacific region and it is expected to grow at the highest rate due to increased healthcare
awareness, improved economic growth, low operating cost and increased outsourcing.
Significant new product launches, collaborations and partnerships joint ventures are some of
the industry trends that are playing a major role for the market growth.

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Some of the major companies that manufacture 3D cell culture products include, Becton
Dickenson & Company (U.S.), Thermofisher Scientific (U.S.), Corning (U.S.), Lonza
(Switzerland), Merck KGaA (Germany), Kuraray Co. Ltd. (Japan), Insphero (Switzerland),
Nano 3D Biosciences (U.S.), JSR Corporation (Organogenix Inc.) (Japan), and Organovo
Holdings Inc. (U.S.).

Emerging players in the 3D cell culture market include, 3D Matrix Co (Japan), AMSBio LLC
(U.S.), Ascendance (U.S.), BioCellChallenge (France), Biolamina AB (Sweden),
CellecBiotek AG (Switzerland), CelVivo IVS (Denmark), Cytoo (France), Fujifilm (Japan),
Hurel Corp (U.S.), Nanopahrma AS (Czech Republic), Prodizen (Korea), etc.

FIGURE 1

3D CELL CULTURE GLOBAL MARKET REVENUE,

BY REGION (2016-2024) ($MN)

1,600.0
Represents CAGR % (2017-2024)
1,400.0 XX%

1,200.0 XX%
Market Size ($Mn)

1,000.0
XX%
800.0

600.0

400.0 XX%

200.0

-
2016 2017 2018 2019 2020 2021 2022 2023 2024
North America Europe Asia-Pacific RoW

Source: The Society for In-vitro Biology (SIVB), International Society for Stem Cell Research (ISSCR), American
Type Culture Collection (ATCC), European Collection of Authenticated Cell Cultures (ECACC), Cell Culture and
Bio-processing Asia Congress (CCBPAC), WHO, Industry Expert Interviews and IQ4I analysis

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2 3D CELL CULTURE GLOBAL MARKET, BY

TECHNOLOGY

FIGURE 2

3D CELL CULTURE GLOBAL MARKET REVENUE, BY TECHNOLOGY,

(2017-2024) ($MN)

1,200.0

1,000.0
Market Size ($MN)

800.0

600.0

400.0

200.0

-
2016 2017 2018 2024
Scaffold Free platforms Scaffold based platforms

FIGURE 3

3D CELL CULTURE SCAFFOLD BASED PLATFORMS GLOBAL MARKET

REVENUE, BY TYPE, (2017) ($MN)

Scaffold Free Platforms Scaffold Based Platforms Scaffolds Hydrogels Others

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FIGURE 4

3D CELL CULTURE GLOBAL MARKET REVENUE, BY PRODUCT,

(2017 V/S 2024) ($MN)

2017 2024

Media, Sera and Reagents Scaffolds Cells& tissues Microplates and Others Assay Kits Others

FIGURE 5

3D CELL CULTURE GLOBAL MARKET REVENUE, BY APPLICATION,

(2017 V/S 2024) ($MN)

450.0

400.0

350.0
Market Size ($MN)

300.0

250.0

200.0

150.0

100.0

50.0

-
Basic Toxicity and Tissue Stem cell Drug Gene Cancer
Research Drug Safety engineering research Discovery therapy Research
screening
2017 2024

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FIGURE 6

3D CELL CULTURE GLOBAL MARKET REVENUE, BY END-USERS,

(2017 V/S 2024) ($MN)

800.0

700.0

600.0
Market size ($Mn)

500.0

400.0

300.0

200.0

100.0

-
Academic institues Biotechnology and Others
pharmaceutical
industries
2017 2024

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3 COMPETITIVE LANDSCAPE

3.1 MARKET SHARE ANALYSIS BY MAJOR PLAYERS

FIGURE 7

3D CELL CULTURE GLOBAL MARKET SHARE ANALYSIS,

BY KEY PLAYERS, 2017

BD Thermofisher Scientific Corning Lonza Merck Kuraray Insphero Others

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3.2 COMPANY MATRIX

TABLE 1

COMPANY MATRIX: COMPANY V/S PRODUCT OFFERING

Revenue

Others
Sl. Number of

XX
XX

XX
XX
XX
Company Headquarters ($ million
No. Employees
2017)

1 300 Microns GmbH Germany XX XX

2 3D Biotek, LLC U.S. <2 XX
3 3D Matrix Co. Japan XX XX
4 3Dtro AB Sweden - -
Advanced Biomatrix,
5
Inc. U.S. XX XX
6 AIM Biotech PTE Ltd. Singapore XX XX
Akron Biotechnology,
7
LLC U.S. XX XX
8 amsbio LLC U.K. XX XX
9 Ascendance U.S. XX XX
10 Aspect biosystems Canada XX <10
*Estimated revenue for FY2017 from Q3 2017
**Estimated revenue for FY2016 as results for FY2017 is not available

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4 MARKET ANALYSIS

4.1 DRIVERS AND OPPORTUNITIES

4.1.1 SUBSTANTIAL R&D INVE STMENTS FOR 3D CELL

CULTURE
The 3D cell culture techniques offer various advantages such as providing of dynamic spatial
gradients of soluble factor concentrations, a wider array of cell-cell and cell-matrix
interactions that regulate cell function and behavior differently; and the ability to support
multiple cell types with spatial organization to mimic native environments. The 3D cell culture
models helps in better understanding cellular systems than 2D in vitro models and is
increasingly being used in various applications such as toxicity screening, drug candidate
evaluation, tissue engineering, cancer research etc., as a result there has been increased
interest in development of 3D cell culture based models and various government and non-
governmental organization have began funding projects utilizing 3D cell culture models.

For example, in September 2017, National Institute of Health awarded about $30 million over
two years to a group of researchers who have been working on developing 3D models of
amyotrophic lateral sclerosis and other diseases. Similarly, in October 2015 InSphero AG
received €750,000 funding from EU-ToxRisk consortium for development of organotypic 3D
cell culture models for animal-free hazard and risk assessment of chemicals. Also, various
medical hospitals and university are funded for development of regenerative medicine and
tissue engineering solutions for unmet medical needs using 3D cell culture techniques. Thus,
such substantial R&D investments in the field of 3D cell culture is driving the growth of the
market.

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5 INTRODUCTION

5.1 RESEARCH METHODOLOGY

The research on 3D cell culture global market involved both widespread secondary and
primary research. The secondary research was carried out on company websites, annual
reports, SEC filings, associations, articles, press releases and journals to identify and collect
both the qualitative and quantitative data related to 3D cell culture products. The primary
sources were experts from supply side such as CEOs, directors, and VP’s, and from demand
side such as research directors, principal scientists, and research heads. All the primary
sources were interviewed to acquire and verify critical qualitative and quantitative information
and also to assess the future forecast of all segments of the 3D cell culture global market.
Along with these, the additional information extracted was about the key players in this
industry with their market revenues, products and developments. The factors responsible for
growth and restriction of the 3d cell cultureglobal market along with the future opportunities
were also determined through secondary research and verified through primary sources.

The following illustrative figure shows the market research methodology applied in making
this report on the 3D cell culture global market.

5.1.1 MARKET SIZE ESTIMATI ON

The size of the 3D cell culture global market and its various segments and sub-segments
were estimated and validated through secondary and primary research. All the 3D cell
culture market percentage shares splits, and breakdowns were determined using secondary
sources and verified through primary sources to get the final quantitative and qualitative
data. This data is consolidated and added with detailed inputs along with analysis from IQ4I
and presented in this report.

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FIGURE 8

3D CELL CULTURE GLOBAL MARKET:

TOP-DOWN AND BOTTOM-UP APPROACH

Global Cell Culture

Market

Global 3D cell culture market

Overall
market size
Market share of 3D cell culture segments and CAGR

Regional market shares

Market share of the players in
the overall 3D cell culture
Market share of sub market and sub markets
Segments in regional
markets

Sub segments Top players Market Revenue in the 3D Cell

market size Culture Market: Becton Dickenson & Company
and (U.S.), Thermofisher Scientific (U.S.), Corning
CAGR (U.S.), Lonza (Switzerland), Merck KGaA
(Germany), Kuraray Co. Ltd. (Japan), Insphero
(Switzerland), Nano 3D Biosciences (U.S.),
Organogenix (Japan), Organovo Holdings Inc.
(U.S.).

Top down approach was used to derive the 3D cell culture global market as well as the 3D
cell culture based on technology, products, applications, end-users and geography. Different
percentage splits were applied for different segments of the 3D cell culture market. Even the
geographical splits differ in each of the disciplines. The bottom-up approach was followed for
market share analysis of the key players and thereby deriving the final 3D cell culture global
market value.

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6 MAJOR COMPANIES

6.1 BECTON DICKINSON AND COMPANY

FIGURE 9

OVERVIEW: BECTON DICKINSON AND COMPANY

14,000.0 828.0 1,000.0

774.0

R&D Expenses ($Mn)

Becton Dickinson & Company 12,000.0
632.0 800.0
Revenue ($Mn)
10,000.0
8,000.0 600.0
Founded: 1897
6,000.0 400.0
Headquarters: U.S.
4,000.0
No. of employees: 49,900 2,000.0
200.0
Revenue: $12,093.0 Mn 10,282.0 12,483.0 12,093.0
0.0 0.0
R&D Expenses: $774.0 Mn 2015 2016 2017
Total revenue R&D Expenses

Revenue by Segment ($Mn) Revenue by Region ($Mn)

3,988.0
5,589.0

6,504.0
8,105.0

Medical Life sciences U.S. International

Source: Annual/Financial Reports, SEC Filings, Investor Presentations, Press Releases and IQ4I Analysis

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6.2 MARKET SEGMENTATION

FIGURE 10

3D CELL CULTURE GLOBAL MARKET: MARKET SEGMENTATION

3D Cell Culture Global Market

By Technology By Products By Application By End-Users

Scaffold Based Scaffold Free

Cells and Tissues Basic Research Academic Institutes
Platforms Platforms

Media, Sera Toxicity and Drug Biotechnology and

Scaffolds Hang Drop Method Pharmaceutical
and Reagents Safety Screening
Industries
Agitation Based Microplates and
Hydrogels Tissue Engineering Others
Methods Others

Others Forced Floating Assay Kits Stem Cell Research

Microfluidics Scaffolds Drug Discovery

Natural Scaffolds Cancer Research

Synthetic Scaffolds Gene Therapy

Hybrid Composite

Hydrogels

Others

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6.2.1 ASSUMPTIONS

Parameter Assumptions

The 3D cell culture global market revenue is

estimated to be XX% of the global Cell culture
Market Revenue
market. The value is cross-validated through
bottom-up and top-down approach as well.

The CAGR for the forecasted period (2017-2024) is

assumed to be normalized and the effect of
Market growth inflation, recession, economic downturn, regulatory
or policy changes, or other factors are not
considered.

The market shares of main segments are analyzed

based on the absolute data and primary inputs. In
Market share and size of segments
the case of the data non-availability, the % splits
and sub-segments
are arrived based on service portfolio of key players
and their contribution in 3D cell culture market.

Market share analysis of key players is performed

in two ways that are based on the primary research

Market Share rankings (Market penetration of players) and the

other is based on actual and estimated 3D cell
culture revenues of key players.

Note: Values in some of the segment or sub-segment do not add due to round-off errors.

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