General Biochemistry Laboratory 5. Nitrous acid is a weak acid.

Would you
Monthly Grading Period expect solutions of nitrous acid and nitric
Problem Set acid, of equal concentration, to have the same
pH? Explain.
6. What is the pH of the solution if [OH-]=2.5
I. Concentration Units & Dilution
x 10-7?
1. A sample of 6.45 g cysteine is dissolved in
7. Find the pH for the following solutions if it
80.1 g of H2O. Calculate the %w/w of
contains:
cysteine in the solution. 7.45% (w/w)cys
[a] 1.8 x 10-6 M barium hydroxide
2. Ten milliters of ethanol (=0.789 g/mL) is
[b] 0.150 M acetic acid, Ka=1.8 x 10-5
added to sufficient amount of water to
[c] 0.40 M ammonia soln’, Kb=1.8 x 10-5
produce 100.0 mL solution. The density of
[d] 0.105 M urea, Ka=0.67
the solution is 0.982 g/mL. Find the
[e] 0.027 M lactic acid, Ka=1.38 x 10-4
concentration in:
[f] 0.105 M aniline, pKa=4.60
[a] volume % 10.00%(v/v)
[g] 0.10 M ascorbic acid, pKa=4.10
[b] mass % 8.03%(w/w)
8. Write the formula of the conjugate base of
[c] mole fraction Xethanol=0.033
the following acids:(a)CH3CH(OH)CO2H (b)
[d] molarity 1.71 M
H2PO42- (c) C2H2O4
3. What is the molarity of a solution made
5. Identify the acid-base conjugate pairs in
when water is added to 11.0 g urea
each of the following reactions:
(MM=60.07) to make 100. mL of solution?
[a] HCO3- + HCO3-  H2CO3 + CO32-
4. A solution made by adding 1.63 g of K 2S
[b] +NH3CH(CH2SH)CO2H + H2O 
(MM=110.3) to 10.0 mL of H2O has a volume +
NH3CH(CH2SH)CO2- + H3O+
at 20C of 11.1 mL. Find the molarity &
[c] HHb + HCO3-  Hb- + H2CO3
normality of the solution.
6. Match the following titration curves to
5. Give the molar concentration of HCl with a
corresponding identity of the analyte
specific gravity of 1.18 and has 37.0% (w/w)
purity. MM=36.5 12.0 M
b
7. How would you prepare 250. mL of 0.866 M
HCl starting from a 5.07 M solution? a
8. Calculate the p-values of the following
solutions: [a] 1.56 x 10-9 M Mg2+ [b] 8.76 x
10-7 M H+ ; Calculate the molar
concentrations of [a] pNi=12.6 [b]
pOH=5.39
9. Water is added to 25.0 mL of a 0.866 M
c d
KNO3 solution until the volume of the
solution is exactly 750. mL. What is the
concentration of the final solution?

II. pH, Ka, Kb, Titration Curve

1. Calculate the [H+] and [OH-] for each of
solution:
the following :
[a] 0.76M HNO3
[b] Blood plasma, pH 7.4
[c] Gastric juice, pH 1.8
2. Find the molar concentration of
hydrochloric acid when the pOH of the
solution is 12.01.
3. Find the molar concentration of barium
hydroxide solution with a pH of 9.82.
4. Find the pH of the hydrochloric acid
solution when 5.00 mL of concentrated
hydrochloric acid, with a specific gravity of
1.18 and has 37%(w/w) purity, is diluted I. weak polyfunctional acid
to 2.0 L. II. strong monoprotic acid
III. weak monoprotic acid
 IV. weak base 9. Describe the preparation of 500. mL acetate buffer,
pH=5.0 from solid NaOAc (10.0g) and stock soln’
III. Buffer (Henderson-Hasselbalch of HoAc (95% w/w,=1.0498).
10. What are the components of the buffer solution
equation, Preparation, Buffer Capacity) when:
1. Calculate the pH of the buffer system made [a] 0.30 mol H3PO4 is mixed with
up of 0.150M NH3/0.350M NH4Cl. Kb=1.8 x 0.10 mol NaOH
10-5. [b] 0.20 mol H3PO4 is mixed with
2. The pH of a bicarbonate-carbonic acid buffer 0.30 mol of the base
is 8.00. Calculate the ratio of the [c] 0.20 mol H3PO4 is made to react
concentration of carbonic acid to that of the with 0.40 mol of the base
bicarbonate. [d] 65.0 g potassium primary
phosphate is made to react with
3. What is the pH of the buffer 0.10M
200.0 mL of 3M NaOH. Calculate
Na2HPO4/0.15M KH2PO4? Ka1=7.5 x 10-3, the pH of the solution.
Ka2=6.20 x 10-8, Ka3=4.8 x 10-13. 11. How much H3PO4 and NaOH are needed to
4. What is the pH of a 500. mL buffer prepare a buffer solution containing 0.200 mole
containing 0.250 moles HCO2H and 0.300 HPO42- and 0.500 mole PO43-?
moles HCO2Na? Ka=1.70 x 10-4. 12. Describe the preparation of 500. mL 0.250M
5. What is the final pH of a solution made by phosphate buffer, pH=11.5, from 6M NaOH and
mixing 100 ml of 0.05 M acetic acid and 100 5M H3PO4.
ml of 0.1 M sodium acetate? 13. What initial concentration of aqueous sodium
dihydrogen phosphate and aqueous sodium
6. Refer to the following buffers and the
hydrogen phosphate is needed to prepare 500 mL
corresponding pKa of the acid: a buffer with a pH of 6.89?
sodium hydrogen citrate/disodium hydrogen 14. Propionic acid, CH3CH2COOH, has a Ka of 1.3 x 10-
A 4.76
citrate 5
mol.L-1. What mass of sodium propionate,
B boric acid/ primary sodium borate 9.23 CH3CH2COONa, would have to be added to 1.00 L
C sodium bicarbonate/sodium carbonate 10.93 of 0.500 mol.L-1 propionic acid for a resulting
potassium dihydrogen phosphate/ disodium
D 7.21 solution of pH 5.00?
hydrogen phosphate
tris(hydroxymethyl)aminomethane
15. What is the pH of the buffer prepared by mixing
E hydrochloride/ 8.30 250. mL of 0.200 M benzoic acid and 100. mL of
tris(hydroxymethyl)aminomethane 0.250M NaOH? pKa=4.19
16. Prepare 200. mL of a 0.100 M formate buffer,
ph=4.50, from a 0.200 M stock soln’ of formic
Suggest a conjugate acid-base pair that could be
acid and its salt. Ka=4.27x10-7.
use to prepare the buffer with the given pH. 17. At the physiological pH of 7.4, what is the ratio of
Calculate also the ratio of salt to that of the salt concentration to acid concentration for a
acid: HPO4-- and H2PO4- buffering system if the pKa is
6.8? Which is the acid in this system?
pH Buffer [salt]/[acid] 18. Prepare 500. mL of 0.100 M bicarbonate buffer,
pH=6.00, from 0.100 M stock soln’ of carbonic
9.41 acid and its salt. Ka=4.27x10-7.
7.00 19. Calculate the pH change that take place when a
100. mL portion of (a) 0.0500 M NaOH and (b)
0.0500 M HCl is added to 400. mL of the buffer
6. Suggest a suitable buffer range for each of soln’ containing 0.200 M NH3 and 0.300 M NH4Cl.
the following substances: Ka=5.70x10-10.
A. Lactic acid (pKa=3.86) & its sodium salt: 20. A buffer containing 1.00 M concentrations of HOAc
_____________ and NaOAc has a pH of 4.742. (a) What is the pH
B. HEPES (pKa=8.30) in its zwitterionic form of the soln’ after 0.0100 mol of HCl has been
& its anionic form: _________ added to 1.00 L of the buffer? (b) What is the pH
of the soln’ after the addition of 0.0100 mol of
7. Describe the preparation of a 200. mL of a 0.200 M NaOH? (c) What is the buffer capacity and pH
benzoate buffer, pH=4.7 if your starting materials change for each soln’ [ in (a) and (b)]?
are 80.0 mL of 0.200 M C6H5COONa and 10.0 g 21. A buffered solution of pH = 4.4 is 0.1 M
C6H5COOH (122.13 g/mol). pKa=4.20. benzoic Acid, HC7H5O2, and 0.15 M sodium
8. Describe the preparation of 500. mL of 0.300 M benzoate, NaC7H5O2. After addition of 1.5 mL
acetate buffer, pH=5.0, from 0.300 M NaOAc and of 6.0 M HCl to 250 mL of the buffered
0.300 M HOAc. Ka=1.8x10-5. solution, what is the resulting pH?:
Ka= 6.6 x 10-5
22. What will be the ratio of acid-base pair of
0.115 M glycine solution that has a pH of
8.10? What is the charge of the predominant
form of glycine in the solution? pKa1=2.40,
pKa2=9.80
23. If 5.00 mL of 0.080 M HCl was added to
0.100 L glycine solution, what will be the
new pH of the solution? What is the buffer
capacity?
24. Can a buffer be prepared by adding 5.0 g of
glycine to 0.150 L of 1.04 M NaOH? Justify
your answer.