Scribd
Upload
78 views26 pages

Carbonaceous Constituents: Biochemical Oxygen Demand

1) The document discusses biochemical oxygen demand (BOD), which is a measure of the oxygen utilized by bacteria to oxidize organic matter in a wastewater sample over 5 days. 2) The BOD test involves diluting a wastewater sample, measuring the initial and final dissolved oxygen, and calculating the oxygen used to oxidize organic matter. 3) A BOD test is conducted for 5 days because 60-70% of carbonaceous oxidation occurs within this period, allowing the test to measure carbonaceous demand before nitrification begins interfering.

Uploaded by

SanthoshMBSanthu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
78 views26 pages

Carbonaceous Constituents: Biochemical Oxygen Demand

1) The document discusses biochemical oxygen demand (BOD), which is a measure of the oxygen utilized by bacteria to oxidize organic matter in a wastewater sample over 5 days. 2) The BOD test involves diluting a wastewater sample, measuring the initial and final dissolved oxygen, and calculating the oxygen used to oxidize organic matter. 3) A BOD test is conducted for 5 days because 60-70% of carbonaceous oxidation occurs within this period, allowing the test to measure carbonaceous demand before nitrification begins interfering.

Uploaded by

SanthoshMBSanthu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 26

Carbonaceous Constituents

Biochemical Oxygen Demand

-Pratik Prakash Kulkarni


Department of Civil Engineering
IIT Guwahati
Effect of Oxygen Demanding Wastes
on Rivers
• Chhatrapati Rajaram Sugar mill
– Accidental release of molasses
Carbonaceous Constituents
• Carbonaceous constituents are measured in
terms of BOD, COD or TOC analyses.
• While the BOD has been the common
parameter to characterize carbonaceous
material in wastewater, COD is becoming
more common in most current comprehensive
computer simulation design models.
Biochemical Oxygen Demand
• Sewage contains Organic matter
– Fats, Proteins and Carbohydrates

• Requires common basis for quantification

• The BOD test gives a measure of the oxygen


utilized by bacteria during the oxidation of
organic material contained in a wastewater
sample.
Biochemical Oxygen Demand

• The test is based on the premise that all the


biodegradable organic material contained in the
wastewater sample will be oxidized to CO2 and
H2O, using molecular oxygen as the electron
acceptor.

• It is a direct measure of oxygen requirements and


an indirect measure of biodegradable organic
matter.
Biochemical Oxygen Demand
Organic matter + O 2 
Microorganisms
 CO 2 + H 2O + New Cells + Stable Products

95-99%

60-70%
60-70%

Variation in DO profile during BOD test with duration of incubation at 20℃


Biochemical Oxygen Demand
• To perform this test on industrial waste
generally there is requirement to add
microbial seed.

• This seed is added as sewage or the water


from river or stream or soil sample where the
industry discharges its waste
1.The sample is taken into a BOD bottle of
volume 300mL. Say 10 mL

Aspirator Bottles
BOD Bottle

Dilution water
2. Diluted it with sufficient amount of
dilution water if required. Say 290mL

BOD Bottle

3. Initial DO is to be calculated and final DO after 3days @27⁰C or 5days @20⁰C is


measured

4. Difference between these reading multiplied by dilution factor gives BOD value.
Source Sawyer Mccarty and Parkin chemistry for environmental engineering
Biochemical Oxygen Demand
𝐷1 − 𝐷2
𝐵𝑂𝐷, 𝑚𝑔/𝐿 =
𝑃

When the dilution water is seeded

𝐷1 − 𝐷2 − 𝐵1 − 𝐵2 𝑓
𝐵𝑂𝐷𝑚𝑔/𝐿, =
𝑃

Where:
D1: Dissolved oxygen of diluted sample immediately after preparation, mg/L
D2: Dissolved oxygen of diluted sample immediately after 5day incubation at 20⁰C,
mg/L
B1: Dissolved oxygen of seed control before incubation, mg/L
B2: Dissolved oxygen of seed control after incubation, mg/
f : Fraction of seeded dilution water volume in sample to volume of seeded dilution
water in seed control=1-P=290/300
P: Fraction of w/w sample to total combined volume i.e. 10/300
Biochemical Oxygen Demand

• As the microbial activity depends on


temperature, BOD test is highly depends on
temperature.

• Generally this temperature is chosen as 20⁰C


or 27⁰C.
Biochemical Oxygen Demand

95-99%

60-70%

Variation in DO profile during BOD test with duration of incubation at 20℃


Biochemical Oxygen Demand
• 𝐿𝑡 = 𝐿₀𝑒 −𝑘1𝑡
where, 𝐿₀: Ultimate BOD, mg/L
𝐿𝑡 : BOD remaining at any time, mg/L
𝑘1: 1st order reaction rate constant,1/d
t : time, d
• But 𝐿₀= 𝐿 + 𝑌
• ∴𝑌 = 𝐿₀(1- 𝑒 −𝑘1𝑡 )
• where, 𝑌: BOD Exerted at that time
Biochemical Oxygen Demand
• Rate constant 𝑘1 is dependent on
temperature, it can be calculated as,
• 𝑘1𝑡= 𝑘1 20⁰Ɵ𝑇−20
where, Ɵ=1.047
• The value of Ɵ is temperature dependent
Biochemical Oxygen Demand
1. Determine 1-day BOD and ultimate first stage
BOD for a w/w whose 5- day BOD is 200mg/L
at 20℃. The reaction constant k (base e) =
0.23𝑑 −1 . What would have been the 5-day
BOD if the test have been conducted at 25⁰C?
Given : k1 (to the base e)=0.23/d
BOD5=200mg/L at 20℃
Calculate:
L0
BOD1 at 20℃
BOD5 at 25℃
Biochemical Oxygen Demand
𝑌
Ans: Formula: 𝑌 = 𝐿₀(1 − 𝑒 −𝑘1𝑡 ), 𝐿₀= , 𝑘1𝑇⁰=𝑘1 2001.047 𝑇−20
(1−𝑒 −𝑘1𝑡 )
𝑌 200
1) Ultimate BOD: ∴ 𝐿₀= = = 293𝑚𝑔/𝐿
(1−𝑒 −𝑘1𝑡 ) (1−𝑒 −0.23∗5 )

2)Determine 1-day BOD:𝑌1 = 𝐿₀ 1 − 𝑒 −𝑘1∗1


= 293 1 − 𝑒 −0.23∗1 = 60.1𝑚𝑔/𝐿
3)Determine 5day BOD at 25⁰C:
𝑘125⁰= 𝑘1 2001.047 25−20 = 0.29𝑑−1
𝑌5 = 𝐿0 1 − 𝑒 −𝑘1∗5 =293 1 − 𝑒 −0.29∗5 = 224𝑚𝑔/𝐿
BOD
2. A BOD test was conducted at 20⁰C in which
15mL of waste sample was diluted with
dilution water to 300mL.
Given:
Initial DO of diluted sample D1 =8.8mg/L
Final DO after 5 days D2=1.9mg/L
Initial DO of seeded dilution water B1=9.1mg/L
Final DO of seeded dilution water B2=7.9mg/L
Calculate
5-day BOD at 20⁰C
BOD
𝐷1−𝐷2 − 𝐵1−𝐵2 𝑓
• Ans:Forrmula, 𝐵𝑂𝐷, 𝑚𝑔/𝐿 =
𝑃
300−15
Where 𝑓 = = 0.95
300
15
𝑃= = 0.05
300
8.8−1.9 − 9.1−7.9 0.95
𝐵𝑂𝐷, 𝑚𝑔/𝐿 = = 115.2mg/L
0.05
BOD
Homework
3. The BOD5of a wastewater is determined to be 150mg/L at 20⁰C.
The k is 0.23 per day. What would the BOD8 be if the test were run
at 15⁰C?
Gate 2000
4. The DO in an unseeded sample of diluted waste having an initial
DO of 9.5 mg/L is measured to be 3.5mg/L after 5 days. The
dilution factor is 0.03 and reaction rate constant, k=0.22/day (to
the base e at 20⁰C) Estimate
The 5 day BOD of the waste at 20⁰C
Ultimate Carbonaceous BOD
Remaining oxygen demand waste after 5-days
The 5 day BOD of this waste at 25⁰C assuming a temperature coefficient of 1.047
(Hint dilution factor=P)
Gate 2004:
5. A portion of w/w sample was subjected to
standard BOD test(5d,20⁰C), yielding a value of
180mg/L. The reaction rate constant (to the
base ‘e’) at 20⁰C was taken as 0.18/d.
temperature coefficient is 1.047. The
temperature at which the other portion of the
sample should be tested, to exert the same BOD
in 2.5days, is…
A. 4.9⁰C B. 24.9 ⁰C C. 31.7 ⁰C D. 35.0 ⁰C
Ans: D. 35.0 ⁰C
Why BOD test is conducted for 5th day
• BOD theoretically takes infinite time to go to completion because
the rate of oxidation is assumed to be proportional to the amount
of organic matter remaining.

• Within 20day period the oxidation of carbonaceous matter is about


95-99% and in 5days it is 60-70%

• 20days is long time to wait for test

• After 8-10 days nitrifying bacteria start using oxygen to convert


ammonia nitrogen to nitrite and then to nitrate.

• We are interested only in first stage BOD or carbonaceous BOD so


we have chosen 5days for the test
Why BOD test is conducted for 5th day
Why BOD test is conducted for 5th day
• Carbonaceous demand can be obtained by
supressing nitrifying bacteria by adding
growth inhibiters of nitrifying bacteria.
• Also this test is conducted on 5th day so as to
ensure less population of nitrifying bacteria.
BOD
• This test can be used to
1. Determine amount of oxygen required to
stabilize organic matter biologically
2. Determine size of treatment facility
3. Measure the efficiency of treatment facilities
4. Determine compliance with wastewater
discharge permits
Thank You

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy