Laboratory Operation
Laboratory Operation
Match the type of automated chemistry analyzer on the left with its definition on the
right:
Your answers are on the left. The correct answers are on the right and highlighted.
Feedback
Drift
Shift
Random errors
Bias 1-2 SD on either side of the mean
Feedback1
Given the following scenario, calculate the equipment cost per test performed:
A new chemistry analyzer costs $108,000.00 and will be expected to run specimens
for 6 years in the clinical laboratory. The maintenance costs are approximately
$1000.00 per year on this analyzer. The hospital laboratory expects to perform
approximately 1200 test per month on this analyzer. What is the equipment cost for
each test performed during the 6 year period?
$2.69
$0.39
$1.89
$1.32
Feedback4
The operating costs of the instrument over six years = $114,000 (analyzer cost +
maintenance fees)
Amount of tests run in 6 years = 1200 test per month x 12 months x 6 years = 86,400
The data showed that the more hours a student studied before the test, the higher the
score he or she was likely to get.
Independent
Dependent
Feedback2
In this case, the score is the dependent variable. The dependent variable is often the
quantity you want to measure; it the result of the experiment or test.
The hours a student studied is the independent variable in this case. It is the quantity
that is directly controlled by the observer or experimenter.
You answered the question correctly.
Feedback4
Which of the following procedures would you employ to monitor the precision of an
assay:
Feedback4
Feedback2
*Note in this question both sides were counted without separate values given for the
average to be obtained, therefore substitute the total cell count in the numerator with
the total squares counted in the denominator
Which infection control measure should be used by a healthcare worker who will be
collecting a blood specimen from a TB patient?
administrative controls
environmental controls
respiratory protection controls
Feedback3
Respiratory protection controls are used for situations with a high risk of exposure.
Feedback3
Measuring the turnaround times of troponin tests from the emergency department
would provide valuable information. This data represents circumstances in which
patient safety may be compromised if results are delayed and treatment is not started
as soon as possible.
Feedback3
Sitting is not a natural position for the body. Muscles will tire if you sit for long
periods of time. Take breaks about every 20 minutes to stand up and walk around.
Steroids
Enzymes
Drugs
Hormones
Feedback3
Thin-layer chromatography has the advantage of being able to test for a large number
of drugs at the same time.
Feedback1
Identify the phase of the total testing process in which each of these errors occurs.
Your answers are on the left. The correct answers are on the right and highlighted.
Feedback
Which of the following is a routine staining technique used in the clinical laboratory:
Feedback4
Wright, Gram, and Romanowsky stains are all commonly used in various areas of the
clinical laboratory.
Feedbackw2
Feedback3
Antithrombin serves as a coagulation inhibitor. When it binds with heparin,
coagulation inhibition is made much stronger. This interaction is the reason why
heparins are utilized as medications to produce anticoagulation (prevent coagulation).
In order to make a 1:10 dilution with 500 microliters of serum, the technologist would
have to use how much saline?
0.0045 mL
0.045 mL
0.45 mL
4.5 mL
49.5 mL
Feedback4
500 microliters of serum is equal to 0.5mL. Therfore, to make a 1:10 dilution with
0.5mL of serum, 4.5mL of saline must be added. 0.5mL = 1 part, so 9 parts of saline
needed = 4.5 m
Feedback2
Red = Fire hazard Yellow = Reactivity hazard Blue = Health hazard White = Specific
hazard
In absorption spectrophotometry:
Feedback4
Feedback
The most common type of osmometer uses freezing point depression. It is also known
as a cryoscope. Other types of osmometers can use either vapor pressure, or colloid
osmotic pressure.
Which of the following infectious agents represent the greatest risk to the laboratory
worker:
AIDS
Meningitis
Hepatitis
Influenza
Feedback
While AIDS is a more serious health threat, hepatitis is far more prevalent, and
therefore represents a greater overall risk.
Which of the following forms of microscopy is not commonly used in the clinical
laboratory:
Darkfield microscopy
Brightfield microscopy
X-Ray microscopy
Flourescence microscopy
Feedback
Brightfield microscopy is, of course, the most common form of microscopy in the
clinical laboratory; darkfield and flourescence microscopy are also used for specific
applications.
True
False
Feedback
The quality of a test to show a positive result in the presence of the disease it was
designed to detect is known as:
specificity
sensitivity
precision
Feedback
Sensitivity is the degree to which a laboratory test can correctly identify the presence
of a disease in a person who actually has it.
The accuracy of a test is its ability to discriminate between the positives and the
negatives. Two parameters of test accuracy are specificity and sensitivity.
Feedback
There is currently no vaccine that will protect against HCV infection.
Approximately 75 to 85% of individuals who are infected with Hepatitis C and
seroconvert develop a chronic form of the disease and about 70% of the
chronically-infected individuals will develop active liver disease.
HCV is primarily transmitted through blood and body fluids.
Individuals who are chronically infected with hepatitis C may have no
symptoms for many years.
You answered the question correctly.
What is ergonomics?
The correct answer is highlighted below
Feedback
Ergonomics is a scientific discipline that studies human capabilites in relation
to work demands.
Physicial ergonomics is the body's responses to physical and physiological
demands.
You answered the question correctly.
What are fires called that involve combustible materials such as paper, wood,
or cloth?
The correct answer is highlighted below
Class A
Class B
Class C
Class D
Feedback
Class A fires include ordinary combustible materials. These are the MOST
common type of fire and are designated Class A. These occur when a solid,
organic material such as paper, wood, cloth, rubber, or some plastics become
heated to their ignition point.
You answered the question correctly.
As defined by a Gaussian distribution curve, what percentage of values would
be expected to fall within two standard deviations of the mean:
The correct answer is highlighted below
75%
85%
95%
100%
Feedback
1 SD = 68.3%, 2 SD = 95.5%, 3 SD = 99.7
You answered the question incorrectly.
According to the OSHA Bloodborne Pathogens Standard, when should work
surfaces in the laboratory be decontaminated with an appropriate disinfectant?
(Choose all that apply)
The correct answers are highlighted below
If blood or other potentially infectious material has spilled on the work surface
Once a day if noticeably contaminated
Upon completion of procedures or the end of the work shift
At least once a week
Feedback
The Bloodborne Pathogens Standard states that work surfaces must be
decontaminated with an appropriate disinfectant when surfaces are noticeably
contaminated or after any spill of blood or other potentially infectious
materials, and after completion of procedures or at the end of the work shift.
You answered the question correctly.
Within what period of time after performance of the tuberculin skin test (TST)
should the result be read?
The correct answer is highlighted below
Immediately
12-24 hours
48-72 hours
5-7 days
Feedback
The result is read 48 - 72 hours after performance of the TST.
You answered the question correctly.
The positive square root of the variance of a set of values is called:
The correct answer is highlighted below
Median value
Mean value
Standard deviation
Coefficient of variation
Feedback
The standard deviation measures the spread of the data about the mean value.
It is useful in comparing sets of data which may have the same mean but a
different range. The standard deviation is calculated by taking the positive
square root of the variance of a set of values.
Conduct the procedure using the chemical fume hood and appropriate personal
protective equipment.
Put on gloves and safety goggles and conduct the procedure on the technical
workbench.
Put on gloves, safety goggles, and a laboratory apron, and conduct the procedure
on the technical workbench.
Feedback
The best practice would be to conduct the procedure in the chemical fume
hood so that the vapors are drawn away from your face. All appropriate
personal protective equipment must be used as well.
You answered the question incorrectly.
Which choice best describes the proper lifting procedure when lifting a heavy
item that will help to prevent back injuries?
The correct answer is highlighted below
Jerk the item up quickly to get to a full standing position.
Keep your legs straight while lifting the item.
Keep the item close to the body, bend the knees, and lift with the legs.
Stand next to the item to be lifted, grasp it by its sides, and swing it around in
front of you.
Feedback
To pick up the item, secure it firmly in your hands, keep the item close to the
body, bend your knees, keeping your back in its natural arched position, and
lift with your legs; leg muscles have more power than the smaller muscles in
the back. The object to be lifted should be directly in front of you. Lift it
straight up, using a smooth motion. Avoid asymmetric lifting (twisting while
lifting).
Feedback
Westgard rules are exclusively used for evaluating quality control results.
What section of the MSDS would provide information about whether a chemical is
carcinogenic?
The correct answer is highlighted below
Feedback
According to OSHA, carcinogens are agents that can cause cancer. In industry, there
are many potential exposures to carcinogens. Generally, workplace exposures are
considered to be at higher levels than for public exposures. Material safety data sheets
(MSDSs) should always contain an indication of carcinogenic potential and this
information will be located in the health hazard data section.