Shimadzu FTIR 8400s Manual
Shimadzu FTIR 8400s Manual
FTIR-8400S
Shimadzu
F o u r i e r Tra n s f o r m
I n f r a re d S p e c t ro p h o t o m e t e r
Discover the Advantages of the FTIR-8400S
FTIR-8400S
S h i m a d z u Fo u r i e r Tra n s fo r m I n f ra r e d S p e c t r o p h o t o m e t e r
D i s c ove r t h e A d va n t a g e s o f t h e F T I R - 8 4 0 0 S
Shimadzu was the first company to offer dynamic alignment as a standard feature in an
affordable FTIR spectrometer. With state-of-the-art technology, the Shimadzu dynamic alignment
system continuously maintains optimal alignment during data acquisition, ensuring consistently
reproducible spectra without the need for tedious mechanical adjustments.
FTIR-8400S is combined with the IRsolution - a 32 bit high performance FTIR software - to
analyze your samples easily and securely.
FTIR-8400S
[M a x i m u m r e s o l u t i o n o f 0 . 8 5 c m - 1 . E x c e l l e n t s i g n a l - t o - n o i s e o f 2 0 , 0 0 0 : 1 o r b e t t e r .]
D y n a m i c a l i g n m e n t e n s u r e s r e p ro d u c i b i l i t y by m a i n t a i n i n g o p t i mu m a l i g n m e n t
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Ve rs ati l i ty
Versatility 1
[Interface with a wide variety of sampling accesories. Microscopy and automation options are available.]
Match the versatile FTIR-8400S with automation and microscopy options, as well as a wide variety of Shimadzu
and other commercially available accessories, to easily accommodate a myriad of sample types.
Left Side : System I (High Pressure Device type) Infrared spectrum of diphenhydramin hydrochloride(powder sample)
Right Side : System H(ViewIR type)
measured with DuraSampllR2A (ATR-corrected spectrum)
The DuraSampllR2A has two types of diamond prism, a diamond
prism supported by a ZnSe lens and a diamond prism supported
by a KRS-5 lens. The ZnSe type can measure up to around
600cm-1. On the other hand, the KRS-5 type can measure up to
around 400cm-1. The infrared spectrum of diphenhydramin
hydrochloride measured with the diamond prism supported by a
KRS-5 lens is shown above. The KRS-5 type can give the infrared
spectrum without noise up to 400cm-1. Plastics, rubbers, files and
liquid samples can be measured with the DuraSampllR2A.
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Ve rs ati l i ty
Versatility 2
For Liquid samples
For measuring liquid samples, demountable or AT R - 8 2 0 0 H A (P/N 208-97240-91)
sealed liquid cells may be used. Additionally, the
A liquid sample trough plate and a solids flat
ATR (Attenuated Total Reflectance) accessories
plate prisms are available to conveniently
are also convenient.
accommodate a range of liquid, solid and film
To measure liquids with ATR, sample preparation
samples. The ZnSe prism is standard in most
is as simple as dispensing the liquid onto the
accessories. A Ge prisms is available for high
prism of the accessory. The ATR correction
refractive index samples such as black
program in the IRsolution software converts the
rubber.Both the ZnSe prism and the Ge prism
measured ATR spectrum into a normal
are water resistant, so even aqueous solutions
transmittance measurement by correcting for
can be easily and conveniently measured.
depth penetration differences.
F o r p ow d e r s a m p l e s
For measuring powder samples, conventional D R S - 8 0 0 0 A (P/N 206-62301-91)
KBr pellet methods may be used. The Diffuse
Sample is mixed with KBr powder, placed in a
reflectance method is also convenient and
sample cup and measured.With the Kubelka-
requires less sample preparation. In the Diffuse
Munk conversion in the IRsolution software, the
reflectance method, the sample is mixed with
diffuse reflected spectra can be converted into
KBr powder and then measured, there is no
transmission spectra for comparison purposes.
need for making a pellet. Single Bounce ATR
accessories may also be used for powder
samples in some cases.
For gases
Various gas cells are available for gas sample
measurement. The path length is selected
according to concentration of the gas. Various
path length cells are available ranging from 5cm
to 20m.
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For solid samples, films, cast resins
The transmission method is effective for film AT R - 8 0 0 0 A (P/N 206-62303-91)
samples up to 100μm thick. For films thicker
This accessory can measure film samples
than 100μm, multi layer film and cast resins, ATR
clamped firmly on one or both sides of the prism.
(Attenuated Total Reflectance) is recommended.
A large contact area results in good sensitivity.
Samples are clamped to the prism to ensure
The KRS-5 prism is standard, a Ge prism is also
contact. With the ATR method, a spectrum is
available for samples with a high refractive
taken through a depth of a few μm within the
index.The incident angle on the sample is
sample. For samples with high refractive indices,
switchable between 30/45/60°, allowing for
the Ge prism is suitable. For hard samples, the
accumulation of spectra at different depths.
diamond prism is preferred. With the ATR
correction available within the IRsolution
AT R - 8 2 0 0 H A (P/N 208-97240-91)
software, ATR spectra is converted into a normal
transmittance spectra. Another method for these A liquid sample trough and a solids flat plat prism are available
sample types, involves scratching the surface of to conveniently accommodate a range of liquid, solid and film
the sample and measuring with a diffuse samples. The ZnSe prism is standard in most accessories.
reflectance accessory. A Ge prisms is available for high refractive index samples.
The measuring light is reflected through the prism 10 times.
The sample is clamped firmly to the prism to ensure contact
with an attached clamp and easily adjustable clamp pressure.
S i C S a m p l e r (P/N 200-66750)
This sample preparation accessory has SiC
emery paper that is used to scratch the sample
of interest. The powder of the sample on the
emery paper is directly analyzed with a Diffuse
Reflectance Accessory.
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Ve rs ati l i ty
Versatility 2
For coating films on metals or resins
For coatings, Reflectance is the measurement S R M - 8 0 0 0 A (P/N 206-62304-91)
method is a choice. Selection of Specular
Specular Reflectance accessory with 10°
Reflection or Reflection absorption method is
reflection. Samples are placed on the top of the
dependent on the thickness of the coating
accessory with the measurement side down.
sample.
Analysis of μm thick thin films is possible.
The specular reflectance spectra can convert
into a transmittance spectra with Kramers-Kronig
conversion in the IRsolution software.
R A S - 8 0 0 0 A (P/N 206-62302-91)
Reflection absorption spectroscopy method
accessory with 70° or 75° reflection angle. Analysis of
nm thick thin films is possible. The optional GPR-8000
polarizer allows for higher sensitivity measurement.
When a polarized light beam is incident on a metal
substrate, the phase of the light is changed. In
vertical polarization, the vectors of the polarized
light are opposite to each other, and hence no
stationary waves are produced . In parallel
polarization, the the vectors of the polarized light
meet at the point to produce stationary waves,
which enhances the sensitivity of the measurement.
A c c e s s o r y fo r a u t o m a t e d a n a ly s i s
Various automation accessories, are available to A S C - 8 0 0 0 T (P/N 206-63900)
provide efficient analysis of large numbers of
ASC-8000T is an auto sample changer for
samples.
performing transmission measurements on up to
18 samples. The holder is designed for 13mm
diameter KBr pellets and optional film-holder or
cell plates for Nujol mulls are also available.
The ASC-8000T can be easily controlled by the
IRsolution software.
Applications
D R S - 8 0 1 0 A S C (P/N 206-62308)
DRS-8010ASC are automatic diffuse reflectance
accessories. The DRS-8010ASC accepts up to
24 samples. The DRS-8010ASC can be easily
controlled by IRsolution software.
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Versatility 3
Space Efficient Laptop PC Control
A unique laptop PC rack option (note1) reduces the
system footprint to just 630mm (W) by 592mm (D),
and the height to only 530mm. Process your data
anywhere with this portable PC option.
Easy Operation
The simplicity of the software makes it perfect for routine work within QA/QC departments or educational
institutions, the main procedure is to operate some limit number of functions. In either case, procedures can be
simplified by standard Easy-Scan program, all procedures including the setting of measurement parameters,
analysis of the sample, data processing, saving, and printing the results are performed with one click operation.
BKG Measurement
Peak Detection
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Software
IRsolution
IRsolution software provides windows based 32bit software control for the FTIR-8400S. With IRsolution, operations
for FTIR analysis can be performed easily and quickly using dedicated analysis screens
Spectrum measurement
Data display and comparison to other spectra
Data processing, quantitation and spectral searching
Report Generation
As the operation progresses, the IRsolution software automatically advances into the optimum mode. Optional
software such as PLS quantitation modules are available for increased versatility.
You can quickly access the many useful functions of IRsolution by customizing the User Interface of software.
Status Monitor
View Mode
After the data is acquired, you can use the View Mode to
zoom in on certain areas or compare with other spectra.
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Data Manipulation, Quantitation and Spectrum Search
After the data acquisition has been completed, you can extract
a variety of specific information from measured spectrum.
The IRsolution software has many functions ranging from Peak
detection, spectral subtraction, Kubelka-Munk conversion and
ATR correction functions to optional Spectrum searching and
Quantitation. The results of data processing can be imported
into Word Processing and Spread
Sheet software applications.
Pesk Picking
Spectrum Search
Quantitation
Quantitation
In addition to multi-point calibration curve methods, the IRsolution
software offers MLR (Multi Linear Regression) and optional PLS
(Partial Least Squares) analysis that is useful for NIR measurements.
Spectrum Search
The IRsolution software allows the creation of user libraries from
acquired spectra, and spectral searching of user and commercial
libraries.
Report Generator
Data is not useful unless you can get the
results in the desired format.
The IRsolution software includes a powerful
report generator with customized layout
features. With the Report Generator, you can
create reusable print templates using any
data including the spectrum, calibration
curve, quantitation results and peak pick
tables. You can set the thickness and color of
the graph lines as well as the font size.
Annotations may be attached on the graph to
comment on the results.
The print layout can be saved as a template
file for later use, ensuring accurate repeat
reporting.
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Va lid ati on Re l i a b i l i t y
Reliability 1
FTIR-8400S has many features on hardware and within the software that improve reliability. Measurement
conditions of the FTIR-8400S are constantly monitored and may be reported at anytime.
IRsolution is complied to GLP and FDA 21 CFR Part 11. note1)
note1) IRsolution Agent software is needed to fully comply to FDA 21 CFR Part 11 regulation.
note2) The electronic signature should be done by IRsolution Agent software for FDA 21 CFR Part 11. Operation Log
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H a rdw a r e R e l i a b i l i t y
Reliability 2
The Interferometer is one of the most important parts of the FTIR. For accurate data collection, the interferometer
must scan with high precision. The FTIR-8400S includes the Patented FJS system which scans the moving mirror
with a smooth and stable mechanism. The optimization and stabilization of the FTIR-8400S interferometer by
Dynamic Alignment system ensures consistent and repeatable results. Since the beam splitter within the
interferometer is susceptible to moisture, the optical system of the FTIR-8400S is sealed and desiccated.
Laser detector
Sealing case
Blue : Spectrum Before Atmosphere Correction Red : Spectrum After Atmosphere Correction
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Optional Software
Optional Software
Wide varieties of optional software to enhance IRsolution capabilities
Powerful 32-bit Windows based software - IRsolution - has various optional software modules to enhance its performance:
IRsolution Agent
Mapping software - AIM-MAP (for the AIM-8800 Microscope)
PLS quantitation software
Curvefitting (Peak split) software
PharmaReport Program note)
Macro Platform
IRsolution includes a wide selection of data manipulation operations which include:
spectral subtraction and Kubelka-Munk conversion; Variety of Quantitation operations - Multi-point Method and Multiple Linear Regression
(MLR) method; as well as Spectral search functions. The optional software for IRsolution expands the application capabilities of IRPrestige-21.
note) In order to use PharmaReport Program, the separately available Macro Platform is required.
I R s o l u t i o n A g e n t (P/N 206-21600-92)
IRsolution Agent software is an Add-On software module for
IRsolution to meet FDA 21 CFR Part 11 regulations and/or to
manage obtained data. IRsolution Agent software consists of a
database function and a data registration function from
IRsolution to IRsolution Agent, as well as electronic signature
capabilities.
M a p p i n g P ro g r a m A I M - M A P (P/N 206-72332-91)
Mapping Software - AIM-MAP - captures visual images from
the AIM-8800 microscope and creates a large composite
visual image of your sample. Mapping conditions such as
Mapping area, Scan intervals, Background position and etc.
are set up on the composite image. You can operate the AIM-
8800 microscope and AIM-MAP through simple point and click
operations. AIM-MAP has 3 types of mapping modes - Area
mapping, Line mapping and Random mapping. Not only can
traditional transmittance/reflectance measurements be done,
but also micro-ATR mapping with an optional ATR objective
are available. IRsolution mapping has various data
manipulation functions such as extracting a spectrum from
mapped data or reprocessing functional-group mapping.
The mapped data can be shown as 3D bird's-eye view,
counter plot or spectral overlay mode.
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PLS Quantitation Software (P/N 206-72331-91)
PLS (Partial Least Squares) quantitation is a widely used
Chemometrics method like Multiple Linear Regression (MLR) method
for quantitation of multicomponents.
IRsolution has PLS I and PLS II method for PLS quantitation. IRsolution
analyzes PLS calibration curves by calculating "Influence", "Residuals",
"Scores", "Loadings", "Press Values", etc.
Curve fitting (Peak split) result of peak (3150-2750cm-1) of ABS resin spectrum.
Top : Target peak and specified peaks
Bottom : Component peaks and a synthesized peak
M a c ro P l a t fo r m (P/N 206-72330-91)
Macro Platform is a module to run Macro programs provided by Shimadzu on IRsolution software. Please contact your Shimadzu
representatives when you need an automatic measurement system with an automatic sample changer or to do routine operation with repeated
specified operations.
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Specifications Dimensions
Interferometer Michelson type with 30° incident angle, dynamic
With desktop PC (Unit : mm)
alignment, sealed and desiccated
Optical system Single beam optics 305
Beam splitter Germanium-coated KBr plate
Light source High brightness ceramic
326
584
Detector Temperature controlled high sensitivity detector
(DLATGS detector)
S/N ratio Greater than 20,000:1 (KRS-5 window, 4cm -1 ,
1 minute, 2200cm-1, P-P)
Wavenumber range 7,800cm -1 - 350cm -1 622
460
Resolution 0.85, 1, 2, 4, 8, 16cm -1
Mirror speed 3 steps; 2.8, 5, 9mm/sec
Data sampling He-Ne laser
Sample compartment W200mm x D230mm x H170mm
250
Data processing Arithmetic operation between spectrum and constant,
functions Arithmetic operation among spectra, Spectral subtraction,
Peak detection, Smoothing, Derivative, Baseline correction,
Data correction, Normalization, Kubelka-Munk conversion,
Kramers-Kronig analysis, ATR correction
Optional programs IRsolution Agent, PLS quantitation, Curve fitting, With laptop PC using rack (Unit : mm)
Mapping, Macro platform, PharmaReport Program
Validation programs Compliance to Japanese/European Pharmacopoeia 630
and ASTM 320
GLP/GMP Operation log recording, Software protection by
user name/password, Management of user level
260
FDA 21 CFR Part 11 (note1) Electronic signature, Data manipulation history
592
recording, Saving of measured interferogram/
background spectrum/manipulated spectra
Ambient conditions 15 - 30°C
Less than 70%
Power requirement AC100/120/220/230/240V 50/60Hz 160VA (note2)
(note1) IRsolution Agent software is needed to fully comply to FDA 21 CFR part 11.
(note2) PC requires additional power.
530
PC Specifications 260
JQA-0376