wk7

Reg. No. :

Question Paper Code : 27076

B.E./B.Tech. DEGREE EXAMINATION, NOVEMBER/DECEMBER 2015.

Second Semester

Biotechnology

QP
BT 6202 — MICROBIOLOGY

(Regulations 2013)

Time : Three hours Maximum : 100 marks

Answer ALL questions.

PART A — (10 × 2 = 20 marks)

1.

2.
E
Define microbiology and microorganisms.

What is TEM?
CO
3. What is the connection between virus and cancer?

4. What processes are involved in bacteriophages assembly?

5. Define nutrition and nutrients based on need and quantity.

6. Define growth factors with examples.

7. Describe the basic effects of temperature on microbes.

8. What antimicrobial processes are inhibited in the presence of extraneous

AU

organic matter.

9. Identify major methods of food preservation

10. What is meant by biotransformation?

PART B — (5 × 16 = 80 marks)

11. (a) Explain the main features of the scientific method, and differentiate
between inductive method and deductive reasoning between hypothesis
and theory.
Or
(b) Compare and contrast three common laboratory techniques for
separating bacteria in a mixed sample.
 wk7

12. (a) Describe 10 unique characteristics of viruses.

Or
(b) Describe the various stages of the multiplication of bacteriophages.

13. (a) Discuss different types of autotrophs and their energy sources.

Or
(b) Describe the process of population growth and how it is measured.

14. (a) Briefly explain how the type of microorganisms present will influence the
effectiveness of exposure to antimicrobial agents.

QP
Or
(b) Explain the desirable features of antimicrobial agents and what are the
factors that influence their effectiveness.

15. (a) Describe industrial fermentation and the role of the fementor.

Or
(b) Describe nitrogen fixation, ammonification nitrification and
denitrification and how microbes are involved in these process.
E ————––––——
CO
AU

2 27076
 WS6

Reg. No. :

Question Paper Code : 71485

B.E./B.Tech. DEGREE EXAMINATION, APRIL/MAY 2017.

Second Semester

Biotechnology

QP
BT 6202 –– MICROBIOLOGY

(Regulations 2013)

Time : Three hours Maximum : 100 marks

Answer ALL questions.

PART A — (10 × 2 = 20 marks)

1.

2.
E
Write the contributions of Louis Pasteur.

What is numerical aperture?

CO
3. Name any four major characteristics of microorganisms.

4. Give the functions of fimbriae.

5. Define selective media.

6. How do you calculate doubling time?

7. Mention the role of HEPA filter.

AU

8. What is decimal reduction time?

9. Enlist any two organic acids produced by fungi.

10. State the importance of Bacillus thuringiensis.

PART B — (5 × 16 = 80 marks)

11. (a) What are stain and dye? Describe the various differential staining
methods. (16)
Or
(b) Give an account on Koch postulates and explain about theory of
spontaneous generation and germ theory of diseases. (16)
 WS6

12. (a) Describe in detail the various steps involved in replication of lysogenic /
temperate phage with illustration. (16)

Or
(b) Discuss the classification and reproduction of fungi in detail. (16)

13. (a) How do you classify micro-organisms on the basis of nutritional

requirements? What is the elementary composition of bacteria and
discuss their physiological role. (16)

Or
(b) Define bacterial growth and discuss various phases of it. Add a note
on chemostat and turbidostat. (16)

QP
14. (a) Briefly discuss each of the following treatments used to control microbial
growth : (i) autoclaving (ii) Pasteurisation (iii) Filtration (iv) Ionising
radiation. (4 × 4)

Or
(b) Name four commercially available antiviral drugs and give details on
various anti-viral agents with their mode of action. (16)

15. (a)
E
Discuss on the production of secondary metabolites and their biological
applications in industry. (16)
CO
Or
(b) Write notes on: (i) Biofertilizer (ii) Biopesticide (8 + 8)

————––––——
AU

2 71485
 Sp 1

Reg. No.

Question Paper Code : 77040

B.E./B.Tech. DEGREE EXAMINATION, APRIL/MAY 2015.

Second Semester

Biotechnology

BT 6202 — MICROBIOLOGY

QP
(Regulation 2013)

Time : Three hours Maximum : 100 marks

Answer ALL questions.

PART A — (10 × 2 = 20 marks)

1. What is the principle of acid fast staining?
2.
3.
4.
What are yeasts?
What is flagella?
E
What is scanning electron microscopy?
CO
5. What is a microbiological medium?
6. Define bioenergetics.
7. What are antibiotics?
8. What is synchronous growth?
9. What is a biosensor?
10. What are biofertilizers?
PART B — (5 × 16 = 80 marks)
AU

11. (a) Describe the following

(i) Gram’s staining (4)
(ii) Flagallar staining (4)
(iii) Capsular staining (4)
(iv) Fungal staining. (4)
Or
(b) Discuss the following
(i) TEM applications to study bacteria. (8)
(ii) Classification of microorganisms. (8)
 Sp 1

12. (a) Describe the classification, structure and multiplication of Algae. (16)

Or

(b) Describe the structures of

(i) Bacteriophages (8)
(ii) Fungi. (8)

13. (a) Describe a bacterial growth curve and its applications. (16)

Or

(b) Describe anaerobic bacterial metabolism with an example. (16)

QP
14. (a) Describe the following
(i) Chemical control of microorganisms. (8)
(ii) Antifungal agents. (8)

Or

(b) Discuss with a suitable example

(i)
(ii)
E
Anti bacterial agents.
Clinically important microorganisms.
(8)
(8)
CO
15. (a) Write notes on
(i) Food preservation (8)
(ii) Vit B12 production using microorganisms. (8)

Or

(b) Write notes on

(i) Bioleaching (8)
AU

(ii) Biogas. (8)

—————————

2 77040
 ws 19

Reg. No. :

Question Paper Code : 80159

B.E./B.Tech. DEGREE EXAMINATION, NOVEMBER/DECEMBER 2016.

Second Semester

Biotechnology

QP
BT 6202 — MICROBIOLOGY

(Regulations 2013)

Time : Three hours Maximum : 100 marks

Answer ALL questions.

PART A — (10 × 2 = 20 marks)

1. In the Gram stain why does alcohol decolourise Gram negative bacteria?

2.

3.
E
Define: Genus, Species and train.

What is a temperate phage? What is the name of its replication process?

CO
4. What is lower fungi and why it is called as lower fungi?

5. Enlist the purpose of differential media.

6. What are organotrophs?

7. Which type of materials require gamma rays for sterilization?

8. Name any two vapor-phase disinfectants.

AU

9. What are antibiotics?

10. Define botulism. Name the organism responsible for botulism.

PART B — (5 × 16 = 80 marks)

11. (a) How do you classify microorganisms? Mention about the methods
employed. (16)
Or

(b) Discuss how gram–positive, gram-negative, acid-fast bacteria and

mycoplasma differ in their cell wall structure and composition along with
their appropriate staining methods. (16)
 ws 19

12. (a) What are the different types of algae? Add a note on life cycle types of
algae. (16)

Or
(b) Describe the different life cycle patterns of Saccharomyce with suitable
illustrations. (16)

13. (a) How the organisms are classified based on its energy requirement and
carbon requirements? (16)

Or

(b) Write an essay on “The anaplerotic mechanisms utilized by bacteria to

replenish intermediates of biochemical pathways. (16)

QP
14. (a) Write in detail about the Bacterial resistance to antibiotics. (16)

Or
(b) Give a detailed note on host –microbe interactions and immunity. (16)

15. (a) Give an account of food preservation and botulism and their control. (16)
Or

(b)
E
Give a brief account on the following (i) Biosensors (ii) Bioleaching
(iii) Biopreservatives (iv) Bioremediation. (4×4)
CO
—————————
AU

2 80159