International Symposium on Applied Chemistry 2015

(ISAC 2015)

PREFACE

One of the main missions of the Indonesian Institute of

Sciences (Lembaga Ilmu Pengetahuan Indonesia – LIPI) is to foster
the scientific researches and to lead the scientific activities in
Indonesia. As a part of the responsibility to the scientific
communities, LIPI is actively supporting scientific activities including
some scientific meetings for both local and global audiences.

In the recent globalization era, scientific achievements play

important role in maintaining sustainable economic development,
and are indeed the foundation of knowledge based society. In the
fields of engineering sciences, in particular, no one can avoid global
competition and on the other hand global collaboration as well. Since
the fields follow universal standards and norms without any local
competitiveness, the engineering scientists should be encouraged to
open their minds globally and to actively interact with their global
peers. To this aim, a global scientific conference is very important to
provide a place for scientific interactions among scientists worldwide.

Starting from this year, the deputyship of engineering

sciences in LIPI supports a conjunction of several existing
conferences in Indonesia, and publishing the proceedings at the
globally indexed publishers. This initiative is intended to attract more
valuable participants from local and abroad. There are 6 conferences
which have agreed to join this initiative, ranging from the fields of
chemistry, physics, informatics, mechatronics, energy, electronics,
telecommunications and appropriate technology. It is hoped that this
would not only gather a wide range of scientists and encourage
interactions among them in the same field, but also across the

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research fields. LIPI would also like to invite local and global
institutions to join this initiative.

LIPI is grateful to all distinguished plenary speakers,

presenters, participants and the scientific reviewers of all
conferences. Since the initiative is new, comments and suggestions
are most welcome and greatly appreciated.

Hope all of you enjoy the conference, have fruitful

discussions and a pleasant stay in Bandung City – the Paris van
Java.

L.T. Handoko
Deputy Chairman for Engineering Sciences
Indonesian Institute of Sciences – LIPI

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COMMITTEE

Advisory Board:

• Prof. Dr. Ir. Iskandar Zulkarnaen (Chairman of Indonesian

Institute of Sciences)
• Dr. Laksana Tri Handoko (Deputy Chairman for Engineering
Sciences of the Indonesian Institute of Sciences)
• Dr. Muhamad Abdulkadir Martoprawiro (Chairman of
Indonesian Chemical Society)
• Dr. Agus Haryono (Director of the Center for Chemistry-
Indonesian Institute of Sciences)
• Dr. Subagjo (Indonesian Catalysis Society)

Scientific and Editorial (S&E) Board:

• Prof. Dr. Silvester Tursiloadi (Coordinator of S&E board,

Research Center for Chemistry, LIPI, Indonesia)
• Prof. Dr. Shogo Shimadzu (Chiba University, Japan)
• Prof. Nico Voelcker (University of South Australia, Australia)
• Prof. Reinout Heijungs (Leiden University, Netherland)
• Dr. Yuni Krisyuningsih Krisnandi (University of Indonesia)
• Prof. Dr. Dong Jin Suh (Korean Institute of Science and
Technology, South Korea)
• Dr. Yessi Permana (Bandung Institute of Technology,
Indonesia)
• Prof. L. Broto Kardono (Ministry of Research and Higher
Education, Indonesia)
• Prof. Dato' Dr. Ir. Abdul Wahab Mohammad (CESPRO,
University Kebangsaan Malaysia)
• Dr. Oaki Yuya (Keio University, Japan)
• Prof. Dr. Arief Budiman (Gadjah Mada University, Indonesia)
• Prof. Muhammad Hanafi (Research Center for Chemistry,
LIPI, Indonesia, Indonesia)
• Prof. Yanni Sudiyani (Research Center for Chemistry, LIPI,
Indonesia)

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• Dr. Habil Andreas Martin (Leibniz Institut fuer tKaalyse

LIKAT, Germany)
• Mikhail A. Salaev, Ph.D. (Tomsk State UniversiR tyu,sia)
• Dr. Ahmad Hanafi Setiawan (Research Center for
Chemistry, LIPI, Indonesia, Indonesia)
• Prof. Dr. Khalid M. Khan (ICCBS, University of Kaacrhi,
Pakistan)
• Dr. Rosi Ketrin (Research Center for Chemistry,PLI,I
Indonesia)
• Dr. Tjandrawati Mozef (Research Center for Chemriys,t LIPI,
Indonesia)
• Dr. Jarnuzi Gunlazuardi (University of Indonesia)
• Dipl. Ing. Haznan Abimanyu, Ph.D (Indonesian Checmail
Society)
• Dr. Arthur Lelono (International Center of Intesrdciplinary
and Advanced Research, LIPI, Indonesia)
• Ir. Edi Iswanto Wiloso, M.ASc. (Research Centerr fo
Chemistry, LIPI, Indonesia)
• Dr. Samuel Toba (American Chemical Society, UnitS edtate
of America)
• Dr. drh. Sulistyani, M.Sc (Bogor Institute of Acgurilture,
Indonesia)
• Prof. Habibah Wahab (Malaysia Institute of Pharmeauctical
and Nutraceutical, Malaysia)
• Dr. Oman Zuas, M.Sc (Research Center for Chemis,trLyIPI,
Indonesia)
• Dr. Wahyu Dwianto, M.Agr. (Research Center for
Biomaterials, LIPI, Indonesia)
• Dr. Ir. Syahrul Aiman (Research Center for Chemriys,t LIPI)
• Dr. Ir. Anny Sulaswatty (Research Center for Chesm triy,
LIPI)
• Dr. Nina Artanti (Research Center for ChemistryI,PLI)
• Dr. Sri Pudjiraharti (Research Center for Chemiys,trLIPI)
• Dr. Sri Hartati (Research Center for Chemistry,PLI)I
• Dr. Jamillah Abbas (Research Center for ChemistrLyI,PI)
• Dr. Dieni Mansur (Research Center for ChemistryI,PLI)

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• Dr. Yenni Meliana (Research Center for Chemistry, LIPI)

• Dr. Indri Badria Adilina (Research Center for Chemistry,
LIPI)

Organizing Committe:

• Chair person : Dr. Nino Rinaldi

• Co Chair person : Dr. Heru Susanto
• General secretary : A. Rahman Setiawan, M.Eng
• Program coordinator : Dr. Arthur Ario Lelono

Members of Organizing Committee:

• T. Beuna Bardant, M.Sc

• Dr. Ahmad Darmawan
• Sujarwo Suryaputra, B.Sc
• Agnes Irwanti, SE, M.Sc
• Th. Ningsi Astuti, SE
• Anniesa Dieni Lestari, S.Ds
• Witta Kartika Restu, B.Eng
• Eni Suryani, SP
• Gian Primahana, M.Sc
• Faiza Maryani, M.Sc
• Sri Fahmiati, M.Sc
• Luthfiana Nurul Hidayati, B.Eng
• Nandang Sutiana, A.Md
• Dian Haelani, B.Sc
• Moch. Muslih
• M. Arifuddin Fitriady S.T.

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EVENT SCHEDULE
SCIENCE & TECHNOLOGY FESTIVAL 2015
ASTON TROPICANA HOTEL
BANDUNG, 5-7 OCTOBER 2015

th
DAY 1 (OCTOBER 5 , 2015)

nd
08.00-09.00 - Registration Alamanda5 (2
Floor)

- Coffee Break Royal Palm 123

th
(8 Floor)

09.00-09.15 Safety Information from Hotel Royal Palm 123

th
Management (8 Floor) MC

09.15-10.00 Opening Session:

1. Report by OC Royal Palm 123

2. Opening Remark by Head of (8th Floor)
Indonesian Institute of
Sciences
MC
3. MoU Signing between Deputy
of Engineering Science LIPI
and CERN (European Council
for Nuclear Research)
4. Photo Session
10.00-12:00 Keynote Speeches

1. Global Collaboration and Its Royal Palm

Importance by Dr. Paolo th
123(8 Floor)
Giubellino - CERN

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2. A d v a n c e d F u n c t i o n a l MC
Material by Prof. Nico
Voelcker – University of South
Australia.

12:00-13.00 Lunch & Exhibition Foyer Alamanda

nd
345 (2 Floor)

13.00 -13.30 Poster Presentation Foyer Royal

th
Palm (8 Floor)

13.30-15.00 Concurrent Oral Session I Royal Palm 123

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5 paper presentations x 3 rooms (8 Floor)

15.00-15.15 Coffee/Tea Break Foyer Royal

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Palm (8 Floor)

15.15-16.30 Concurrent Oral Session II Royal Palm 123

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5 paper presentations x 3 rooms (8 Floor)

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DAY 2 (OCTOBER 6 , 2015)

07.30-08.30 Registration Royal Palm 123

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(8 Floor)

08.30-10.00 Leading Talks I Royal Palm 123

th
1. Ni based alloy catalysts for (8 Floor) MC
chemoselective hydrogenation
of unsaturated carbonyls (Prof.

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Dr. Shogo Shimadzu - Chiba

University, Japan)
2. Hierarchical Structure Control
of Organic and Inorganic
Polymer Materials (Dr. Oaki
Yuya - Keio University, Japan)
10.00-10.15 Coffee/Tea Break Royal Palm 23
th
(8 Floor)
10.15-11.45 Leading Talks II Royal Palm 23
th
1. Biorefinery–Opportunities for (8 Floor)
Sustainable energy,
environment and economic
growth (Prof. Dato' Ir. Dr.
Abdul Wahab Mohammad -
Universiti Kebangsaan
Malaysia, Malaysia)
2. Separation of Toxic Metal Ions
from Other Metal Ions
/Environmental Chemistry
(Prof. Yu Komatsu-Kanazawa
Institute of Technology, Japan)
11.45-12.00 Greeting from Director of Research Royal Palm 23
th
Center for Chemistry, Indonesian (8 Floor)
Institute of Sciences

12.00 - 13.00 Lunch and Exhibition Foyer Alamanda

nd
345 (2 Floor)

13.00 -13.30 Poster Presentation Foyer Royal

th
Palm (8 Floor)

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13.30-15.00 Concurrent Oral Session III Royal Palm 123

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5 paper presentations x 3 rooms (8 Floor)

15.00-15.15 Coffee/Tea Break Foyer Royal

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Palm (8 Floor)

15.15-16.30 Concurrent Oral Session IV Royal Palm 123

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5 paper presentations x 3 rooms (8 Floor)

16.30-16.45 Announcement of Excursion Royal Palm

ORAL PRESENTATION SCHEDULE

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DAY 1 (OCTOBER 5 , 2015) (Royal Palm 1)

Time Paper Paper Titlle Presenter

Code
13.30- Organic Chemistry I
15.00 Chair-person : Dr. YuyaOaki

13.30- 002 Mixed linker metal organic SitiNurhazlin

13.45 framework as an efficient and Jaluddin
reusable catalyst for Suzuki
Miyaura coupling reaction
13.45- 054 Microwave-assisted preparation Ivy Librando
14.00 of 1,3-dialkylimidazolium
bromide ionic liquids and their
utilization in the Fischer indole
synthesis of 1,2,3,4-
tetrahydrocarbazole

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14.00- 074 Microwave-assisted Fischer Ramon M.

14.15 indole synthesis of 1,2,3,4- Eduque, Jr.
tetrahydrocarbazole using
pyridinium-based ionic liquids
14.15- 077 Impact strength and thermal Halimatud
14.30 degradation of Waste dahlia
polypropylene (wPP)/oil palm Nasution
empty fruit bunch (OPEFB)
composites: effect of maleic
anhydride-g-polypropylene
(MAPP) Addition
14.30- 089 Synthesis of 1,4-butanediol Evi
14.45 monooleate and 1,4-butanediol, Triwulandari
9-hydroxy-10-methoxy-
monostearate from palm oil as
modifier of epoxy resin for
coating
14.45- 107 Study on the nitration reaction Haryanto
15.00 of N,N-diphenylamine
compounds and Its
characterization as organic light
emitting diode (OLED) material
15.00-15.15 Coffee break
15.15- Organic Chemistry II
16.30 Chair-person : Ramon M.
Eduque, Jr.
15.15- 061 Performance of M-silicate and Nabila
15.30 M-polystyrenesulfonate (M = Karina Putri
Mg, Ca) in extraction of
carotenoids from crude palm oil
using toluene as a collector
15.30- 113 Structural properties of Siti Ulfa
15.45 Ni/γ−Al2O3 and Cu/γ−Al2O3
catalyst and its application for
hydrogenation of furfurylidene
acetone

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15.45- 119 Enzymatic extract fractionation Lloyd Arvin

16.00 of (Cymbopogon winterianus Malaluan
Jowitt) Citronella through
supercritical carbon dioxide
16.00- 132 The stability of poly Nasrul
16.15 (ethersulfone) membranes Arahman
treated in hot water and
hypochlorite solution
16.15- 133 Two step of methanol synthesis Hendriyana
16.30 from biomass-derived syngas:I.
carbonylation of methanol with
CO in presence potassium
methoxide catalyst
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DAY 1 (OCTOBER 5 , 2015) (Royal Palm 2)

Time Paper Paper Titlle Presenter

Code
13.30- Medicinal Chemistry I
15.00 Chair-person : Prof.
Mohamed El Borai
13.30- 017 In vivo study of ethyl acetate Tjandrawati
13.45 extract of "Sukun" Mozef
(Artocarpusaltilis (Parkinson)
Fosberg) in preventing
atherosclerosis
13.45- 111 Regenerating NADH enhances Fithriani
14.00 ethanol production in aerobic Fithriani
culture of eecombinant
Escherichia coli
14.00- 055 Effect of Ca2+ ion to the activity Rukman
14.15 and stability of lipase isolated Hertadi
from Chromohalobacter
japonicus BK-AB18
14.15- 095 Antiglycation and antioxidant Sagarika
14.30 potentials and total phenolic Ekanayake
contents of decoctions from

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antidiabetic plants

14.30- 108 XPS analysis and antibacterial Hardi Ys

14.45 assay of novobiocin coating
14.45- 123 Potency of Rhizophora spp. Wulan Tri
15.00 extract as antioxidant and Wahyuni
inhibitor of
acetylcholinesterase
15.00- 15.15 Coffee break

15.15- Medicinal Chemistry II

16.30 Chair-person : Siti Nurhazlin
Jaluddin
15.15- 135 Novel synthesis, antimicrobial, Mohamed
15.30 antioxidant and antitumor El-Borai
evaluation of imidazolyl
pyrazolopyridine and
pyrazoloquinoxaline derivatives
15.30- 019 Cloning and sequencing Candra
15.45 haloacid dehalogenase gene Tahya
from Klebsiella pneumoniae
ITB1
15.45- 062 Screening of carbonic Reza
16.00 anhydrase II inhibitor from Aditama
flavones group with docking
and molecular dynamics
simulation
16.00- 064 Antibacterial and antioxidant Melanny
16.15 activity of 1,3-aiaryl-prop-2-en- Sulistyowaty
1-on derivatives
16.15- 060 Isolation and characterization of Dian Siti S.
16.30 organic solvent stable protease
isolated by Pseudomonas
stutzeri BK AB-12

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DAY 1 (OCTOBER 5 , 2015) (Royal Palm 3)

Time Paper Paper Titlle Presenter

Code
13.30-15.00 Environmental Chemistry I
Chair-person : Prof. Nico
Voelker
13.30- 021 Activated carbon from lignin- Feni Amriani
13.45 based black liquor coagulated
by polyaluminium chloride
13.45- 072 Study of softening hard water Uji Pratomo
14.00 using Pistacia vera shell as
adsorbant for calcium and
magnesium ion removal
14.00- 023 Optimization of saccharification Eka
14.15 and fermentation process in Triwahyuni
bioethanol production from oil
palm fronds
14.15- 094 The potential of energy Rina
14.30 efficiency on recycle paper Masriani
bioprocess
14.30- 025 Effect of electrodeposition D.
14.45 reactor type on nickel recovery Djaenudin
from electroplating wastewater
14.45- 016 Alkaline delignification using Muryanto
15.00 black liquor for oil palm pmpty
fruit bunch
15.00-15.15 Coffee break
15.15- Environmental Chemistry II
16.30 Chair-person : Prof. Sagarika
Ekanayake
15.15- 045 Ultrasonic approach in butterfly Bea Subrata
15.30 pea extraction and extract
sterilization by ultrafiltration for
eye drop active ingredient

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15.30- 047 Ultrasound assisted extraction Erico

15.45 of bitter gourd fruit (Momordica Himawan
charantia) and vacuum
evaporation to concentrate the
extract
15.45- 059 The preliminary study of the Ariestya
16.00 dye extraction from the Arbita
avocado seed Using ultrasonic
assisted extraction
16.00- 076 Potential of oil palm empty fruit Lin Marlina
16.15 bunch (EFB) as media for
Oyster mushroom,
pleurotusostreatus cultivation
16.15- 120 Inhibition of microbial growth by Wiratni
16.30 nano-chitosan for fresh tilapia Budhijanto
(Oreochromissp) preservation

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DAY 2 (OCTOBER 6 , 2015) (Royal Palm 1)

Paper Paper Titlle Presenter

Code
13.30- Material Chemistry I
15.00 Chair-person : Prof. Shogo
Shimazu
13.30- 007 Cyclic oxidation of aluminide Eddy Basuki
13.45 coated two phase α2-Ti3Al/γ-
o
TiAl alloys at 1000 C
13.45- 011 Synthesis and characterization Sherly
14.00 of ZnO nanopowders by sol-gel Kasuma
method with various additives Warda
Ningsih
14.00- 029 Fabrication of SiO2-TiO2/PVDF Muhamad
14.15 copolymer nanofiber composite Nasir
by electrospinning process

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14.15- 097 Hydrogenation of biomass- Rodiansono

14.30 derived furfural over highly
dispersed-Aluminium hydroxide
supported Ni-Sn(3.0) alloy
catalysts
14.30- 116 MnO2/CNT as ORR Chee Woon
14.45 electrocatalyst in air-cathode
microbial fuel cells
14.45- 003 Voltammetric determination of Shanty
15.00 Cr (VI) using gold
nanoparticles-modified glassy
carbon electrode
15.00-15.15 Coffee break

15.15- Material Chemistry II

16.30 Chair-person : Prof. Abdul
Wahab
15.15- 058 Synthesis and characterization Hans
15.30 of carbon nanospheres using Kristianto
cooking palm oil as natural
precursors onto activated
carbon support
15.30- 063 Esterification of free fatty acid Arif Hidayat
15.45 on palm fatty acid distillate
using activated carbon catalysts
generated from coconut shell
15.45- 100 Cracking Callophyllum Savitri
16.00 inophyllum L. oil to bio-gasoline Soeraswo
by micro-porous based zeolite
and Al2O3 catalysts
16.00- 114 Effect of CuO nanoparticle on Huei Ruey
16.15 mechanical and thermal Ong
properties of palm oil based
alkyd/epoxy resin blend

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16.15- 134 Effect of regeneration David

16.30 temperature on particle Bahrin
characteristics and extent of
regeneration of saturated SO2-
adsorption of CuO/g-Al2O3
adsorbent

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DAY 2 (OCTOBER 6 , 2015) (Royal Palm 2)

Paper Paper Titlle Presenter

Code
13.30- Organic Chemistry III
15.00 Chair-person : Dr.
Norhanifah Mohd Yazid
13.30- 081 Technology for encapsulation of H. Husniati
13.45 gluten
13.45- 112 Novel method for Evana
14.00 microfibrillated cellulose based Yuanita
on ArengaPinnata "ijuk"
fiber:preparation and
characterization
14.00- 115 Enzymatic conversion of Chi Shein
14.15 glycerol to glyceric acid with Hong
immobilized laccase in Na-
alginate matrix
14.15- 026 Extraction and quantification of Zul Ilham
14.30 toxic compound mimosine from
Leucaena leucocephala leaves
14.30- 084 Evolution of microstructure Agus
14.45 model aluminum based Pramono
composite by novelty process:
repetitive press-roll bonding
14.45- 057 Effect of glycerol as carbon Monica Putri
15.00 source for biosurfactant
production by halophilic
bacteria Pseudomonas stutzeri

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BK AB-12
15.00-15.15 Coffee break

15.15- Green Chemistry

16.30 Chair-person : Prof. Yu
Komatsu
15.15- 066 Characteristics and bond Euis
15.30 performance of wood adhesive Hermiati
made from natural rubber latex
and alkaline pretreatment lignin
15.30- 005 The influence of Norhanifah
15.45 deproteinisation in the Mohd Yazid
morphology of natural rubber
latex particles and subsequent
film formation
15.45- 014 Chemical modification of Arief
16.00 upgraded bentonite as filler of Ramadhan
natural rubber/organobentonite
nanocomposite
16.00- 048 Influence of carbon black and Ika Ulfah
16.15 silica filler on the rheological
and mechanical properties of
natural rubber compound
16.15- 027 Effect of accelerated stability Yenni
16.30 test on characteristics of Meliana
emulsion systems with chitosan
as a stabilizer

th
DAY 2 (OCTOBER 6 , 2015) (Royal Palm 3)

Paper Paper Titlle Presenter

Code
13.30- Analytical Chemistry I
15.00 Chair-person : Mohamad
Raizul Zinalibdin

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13.30- 001 Development semi empirical S Sudibyo

13.45 equation of limiting current for
Lead Magneto
electrodeposition
13.45- 042 A physico-chemical analysis of Chinthani
14.00 coconut shell powder Liyanage
14.00- 098 The initial study of polyaniline Purnama
14.15 with manganese oxides for Ningsih
electrochemical capacitors
14.15- 118 Proficiency testing and N. Nuryatini
14.30 laboratory performance in the
field of pH measurement: An
Indonesian experiment
14.30- 032 Analytical method development Yosi
14.45 for bisphenol A in tuna by using Aristiawan
High Performance Liquid
Chromatography-UV
14.45- 018 Performance test of vitamin B6 T. Beuna
15.00 test kit candidate Bardant
15.00-15.15 Coffee break

15.15- Analytical Chemistry II

16.30 Chair-person : Prof. Dr. Hisao
Kokusen
15.15- 015 A novel molecularly imprinted Mohamad
15.30 polymer acrylonitrile-butadiene- Raizul
styrene membrane for Zinalibdin
adsorption of hippuric acid
15.30- 033 Laboratory performance Yohanes
15.45 evaluation on analysis of Ridwan
cadmium and lead in herb
through participation in the
regional laboratory comparison
APMP.QM-P10
15.45- 038 Characteristics of feed coal and Diah
16.00 particulate matter in the vicinity Lestiani
of coal-fired power plant in

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Cilacap, Central Java,

Indonesia

16.00- 082 Comparison two analytical Harry

16.15 methods for determination of Budiman
propane in gas mixtures
16.15-
16.30

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TABLE OF CONTENTS

PREFACE ............................................................................i
COMMITTEE .........................................................................iii
EVENT SCHEDULE ........................................................................ vi
TABLE OF CONTENTS ......................................................................xx
Nanostructured Silicon in Nanomedicine
Nicolas H. Voelcker* …...................................................................xxxvi

Ni based alloy catalysts for chemoselective hydrogenation of

unsaturated carbonyls
SHIMAZU, Shogo* ......................................................................xxxvii

Biorefinery – Opportunities for Sustainable energy, environment and

economic growth
Abdul Wahab Mohammad, PhD …….................................................xxxviii

Separation of toxic metal ions from other metal ions (environmental

chemistry)
Yu Komatsu .................................................................................xxxiv

Hierarchical Structure Control of Organic and Inorganic Polymer

Materials
Yuya Oaki ...................................................................................xl

[001] Development of semi empirical equation of limiting current

for Lead (Pb) Magneto electrodeposition
a b,
Sudibyo and N. Aziz * ......................................................................1

[002] Mixed linker metal organic framework as an efficient and

reusable catalyst for Suzuki Miyaura coupling reaction
a, b
Siti Nurhazlin Jaluddin *, Karimah Kassim ,Wan Nazihah Wan
a
Ibrahim ..............................................................................................2

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[003] Voltammetric determination of Cr(VI) using gold

nanoparticles-modified glassy carbon electrode
Santhy Wyantuti, Safri Ishmayana, Yeni Wahyuni Hartati .................3
nd
[004] Utilization of distillation residue of 2 generation
bioethanol for fine chemicals production
a, a a
Sabar P.Simanungkalit *, Dieni Mansur , Nino Rinaldi , Robert
a
R.Widjaya ..........................................................................................4

[005] The Influence of Deproteinisation in the Morphology of

Natural Rubber Latex Particles and Subsequent Film Formation
a, b c
Norhanifah M.Y. *, Nurulhuda. A and Asrul. M ...............................5

[006] Recovery of inulin fiber from local red dahlia (Dahlia sp. L)
tuber through enzymatic hydrolysis using Aspergillus sp.-CBS5
and bacillus sp.-cbs6 for functional food
Agustine Susilowati, Aspiyanto, Hakiki Melanie, Jeti Mulyati and Yati
Maryati ........................................................................................6

[007] Cyclic Oxidation of Aluminide Coated Two Phase α2-Ti3Al/γ-

o
TiAlAlloys at 1000 C
a b a
Eddy A. Basuki *, Dita Siti Hajar , Farhan Rahman and Djoko
c
Prajitno ...............................................................................................7

[008] A New Flavonoid Derivative as Cytotoxic Compound

Isolated From Ethyl Acetate Extract of Macaranga Gigantifolia
Merr. Leaves
Akhmad Darmawan, Megawati, Puspa Dewi N. Lotulung, Sofa Fajriah,
Gian Primahana, and Lia Meiliawati .....................................8

[009] Reducing salt from autolysate of fermented mung bean

(phaseolus radiatus l.) using diafiltration-nanofiltration (df-nf)
mode for quality improvement of savory flavor product
Aspiyanto, Agustine Susilowati and Hakiki Melanie ...........................9

[010] Evaluation of culture media for butyrolactone i production

by Aspergillus terreus LS07 AS ANTIDIABETIC

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RiznaTriana Dewi*, Hani Mulyani, Puspa D.N. Lotulung and Nina

Artanti ......................................................................................10

[011] Synthesis and Characterization of ZnO Nanoparticles by

Sol-Gel Method with Various Additives
Sherly Kasuma Warda Ningsih*, Miftahul Khair, Silvi Veronita .......11

[012] Determination of Assigned Values for Cadmium, Cobalt and

Manganese in Drinking Water Proficiency Testing
a
Eka Mardika Handayani *, Isna Komalasari, Rosi Ketrin ................12

[013] Characterization of Inulin from Local Red Dahlia (Dahliasp.

L) Tubers by Infrared Spectroscopy
Hakiki Melanie , Agustine Susilowati, Yety M. Iskandar, Puspa D.
Lotulung and Desak G. S. Andayani ...........................................13

[014] Chemical Modification of Upgraded Bentonite as Filler of

Natural Rubber/Organobentonite Composite
a a
Arief Ramadhan *, Mohamad Irfan Fathurrohman , Bambang
b
Soegijono ........................................................................................14

[015] A novel molecularly imprinted Acrylonitrile-Butadiene-Styrene

(ABS) membrane for absorption of hippuric acid
ab a
Mohamad Raizul Zinalibdin , Abdul Rahim Yacob and Mohd Marsin
a
Sanagi ................................................................................15

[016] Alkaline Delignification of Oil Palm Empty Fruit Bunch

using Black Liquor from Pretreatment
a a a b
Muryanto *, Eka Triwahyuni , Haznan Abimayu , Agung Cahyono ,
b a
Effendi Tri Cahyono , Yanni Sudiyani ...........................16

[017] Bioactivity of Ethyl Acetate Fraction from the leaves of

“Sukun” (Artocarpus altilis (Parkinson) Fosberg) in Preventing
Atherosclerosis

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a a b
Tjandrawati Mozef *, Chandra Risdian , Elin Y. Sukandar , Andreanus
b
A. Soemardji ................................................................17

[018] Performance Test of Vitamin B6 Test Kit Candidate

1 1 1
Teuku Beuna Bardant , Anastasia Fitria Devi , Zatil Afrah Athailah ,
1 2 1
Wirasuwasti Nugrahani , Ahmad Latiful Qolbi , Aspiyanto .........................18

[019] Cloning and Sequencing of Haloacid Dehalogenase Gene

from Klebsiella pneumoniae ITB1
a a
Candra Yulius Tahya , Enny Ratnaningsih * ....................................19

[020] Synthesis and Toxicity Assessments Some para-methoxy

Chalcones Derivatives
a b b
Fitra Perdana *, Yum Eryanti , Adel Zamri .....................................20

[021] Activated Carbon from Lignin-BasedBlack Liquor

Coagulated by Polyaluminium Chloride
a a a a
Feni Amriani , Vera Barlianti , Muryanto , Ajeng Arum Sari ...........21

[023] Optimization of Saccharification and Fermentation Process

in Bioethanol Production from Oil Palm Fronds
a b a
Eka Triwahyuni *, Sri Hariyanti , Deliana Dahnum , Muhammad
b a
Nurdin , Haznan Abimanyu .............................................................22

[024] Effect of Extraction Method on Total Flavonoid, Total

Phenolic Content, Antioxidant and Antibacteria Activity of
Zingiber officinale Rhyzome
a a a
Rina Andriyani *, Thelma A. Budiana , Sri Pudjiraharti ..................23

[025] Effect of electrodeposition reactor type on nickel recovery

from electroplating wastewater
a a a
Djaenudin , Diana Rahayuning Wulan , Hari Rom Hariyadi ...........24

[026] Extraction and Quantification of Toxic Compound Mimosine

from Leucaena leucocephala Leaves

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Zul Ilham*, Hanisjasymah Hamidon, Nurul Amalina Rosji, Norfadhilah

Ramli, Normaniza Osman ............................................25

[027] Effect of Accelerated Stability Test on Characteristics of

Emulsion Systems with Chitosan as a Stabilizer
a
Witta Kartika Restu , Yulianti Sampora, Yenny Meliana, Agus
Haryono* ..........................................................................................26

[028] Formulation of Instant Functional Drinks Based on Ginger

and DFA III
1 1 1
D. Ratnaningrum* , Thelma A. Budiwati , W. Kosasih , and S.
1
Pudjiraharti .....................................................................................27

[029] Fabrication of SiO2-TiO2/PVDF Copolymer Nanofiber

Composite by Electrospinning Process
a b c
Muhamad Nasir , Juliandri , Bambang Prihandoko .......................28

[030] Preparation of Inulin from Dahlia Tubers

a a a
Wawan Kosasih , Sri Pudjiraharti , Diah Ratnaningrum , Rina
a a
Andryani , Sri Priatni .......................................................................29

[031] Effect of alkaline pretreatment on properties of

lignocellulosic oil palm waste
Vera Barlianti*, Deliana Dahnum, Hendris Hendarsyah, and Haznan
Abimanyu ............................................................................30

[032] Analytical Method Development for Bisphenol A in Tuna by

using High Performance Liquid Chromatography-UV
Yosi Aristiawan*, Nurhani Aryana, Dillani Putri, Dyah Styarini ........31

[033] Laboratory Performance Evaluation on Analysis of

Cadmium and Lead in Herb Through Participation in The
Regional Laboratory ComparisonAPMP QM-P10
a b
Yohanes Susanto Ridwan and Willy Cahya Nugraha ...................32

[034] Surface Analysis of Carboxylic Acids Modified SiO2 on

PMMA/ENR 50/LiBF4 Electrolytes

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a a
Siti Izzati Husna Mohd Azuan ,*, Famiza Abdul Latif , Sharil Fadli
a
Mohamad Zamri .....................................................................33

[035] Electrochemical Lipase Biosensor Based On 1-(2-Ethoxy-

Ethyl)-1- Methylpyrrolidinium Bis(Trifluoromethylsulfonyl)imide
ForTriglycerideDetermination In Olive Oil
a a
Nurul Huwaida Rosli , Zainiharyati Mohd Zain *, Nor Monica Ahmad .......34

[036] Occurrence and Removal of Pharmaceutical Products in

Municipal Wastewater
Siti Norbayu Mohd.Subari, Rozita Osman , Halila Jasmani, Norashikin
Saim* ..............................................................................35

[037] Pyrolysis-gas chromatography-mass spectrometry (Py-GC-

MS)analysis of automotive paints traces
Raja Zubaidah Raja Sabaradin, Rozita Osman, Halila Jasmani,
Norashikin Saim* .............................................................................36

[038] Characteristics of Feed Coal and Particulate Matter in the

Vicinityof Coal-fired Power Plant in Cilacap, Central Java,
Indonesia
a a a a
Diah Dwiana Lestiani *, Muhayatun Santoso , Syukri Kurniawati ,
a a
Natalia Adventini , Djoko Prakoso D.A .......................37

[039] Extraction of Citalopram and Main Metabolites In Rabbit

Urine Samples from Fatal Citalopram Intoxication Using
QuECheRS and High Performance Liquid Chromatography
a b
Siti Aisyah Shamsuddin , Rumiza Abd Rashid , Farida Zuraina Mohd
a,b,*
Yusof .................................................................................38

[040] Synthesis of Vanillin Derivatives Using CuCl(OH).TMEDA

Catalyst
a a b
Michelle Ongkosoewito , Hery Sutanto , Yulia Anita * …......................39

[041] Application of mint oil as an antimicrobial agent in the

development of liquid surface disinfectant for food contact
surfaces
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Evita Santoso, Hery Sutanto*, Elisabeth K. Prabawati ....................40

[042] A Physico-Chemical Analysis of Coconut ShellPowder

a b
Chinthani D Liyanage *, Mevan Pieris ............................................41

[043] Development of Validated Method for Determining the

Organochlorine Pesticide Risidues in Lettuce Using QuEChERS
Technique
a* a a
Evita Boes , R. Tina Rosmalina Yohanes Susanto Ridwan , Willy
a a
Cahya Nugraha , and Retno Yusiasih ...................................42

[044] Application of mint oil as an antimicrobial agent in

homemade mayonnaise
a b a
Isabella Kartika Sari , Hery Sutanto , Elisabeth K. Prabawati * ......43

[045] Ultrasonic approach in butterfly pea extraction and extract

sterilization by ultrafiltration for eye drop active ingredient
Bea Anthika*, Samuel P. Kusumocahyo, Hery Sutanto ..................44

[046] Iodination Method of Quercetin for Synthesis of Anticancer

Labelled Compound
a b a
Maula Eka Sriyani , Dian Ayu Utami , Eva Maria Widyasari , Muharam
b
Marzuki ...........................................................................45

[048] Influence of Carbon Black and Silica Filler on the

Rheological and Mechanical Properties of Natural Rubber
Compound
Ika Maria Ulfah*, Riastuti Fidyaningsih, Sri Rahayu, Diah Ayu Fitriani, Dita
Adi Saputra, Dody Andi Winarto, and Lies A. Wisojodharmo ...........47

[049] Biotransformation of eugenol to dehydroeugenol catalyzed

by Brassica juncea Peroxidase and its cytotoxicity activities
a a a a
Yulia Anita , Andini Sundowo , Puspa Dewi N. L , Euis Filailla , Hani
a a a a
Mulyani , Chandra Risdian , Sofna Banjarnahor , Muhammad Hanafi
b
and Enade P istyastono .................................48

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[050] Screening and Characterization of Levan Secreted by

Halophilic Bacterium of Halomonas and Chromohalobacter
Genuses Originated from Bledug Kuwu Mud Crater
a b
Daris Qodarisman Nasir , Deana Wahyuningrum , Rukman Hertadi …....49

[051] Isolation of Cellulose Nanocrystals from Bacterial Cellulose

Produced from Pineapple Peel Waste Juice as Culture Medium
a,b, b b
Budiman Anwar *, Bunbun Bundjali , I Made Arcana * .................50

[052] Elucidation of Active Site Protective Residues in

Rhizomucor miehei Lipase by Targeted Molecular Dynamics
Approach
Yusran Abdillah Muthahari, Rukman Hertadi* ..................................51

[053] Isolation and characterization of levan from moderate

halophilic bacteria Bacillus licheniformis BK AG21
a b a
Mamay , Deana Wahyuningrum , Rukman Hertadi * ......................52

[054] Microwave-assisted Fischer indole synthesis of 1,2,3,4-

tetrahydrocarbazole catalyzed by 1,3-dialkylimidazolium bromide
ionic liquids
a a
Ivy L. Librando * and Evelyn C. Creencia .......................................53
2+
[055] Effect of Ca Ion to The Activity and Stability of Lipase
Isolated from Chromohalobacter japonicus BK-AB18
Rukman Hertadi* and Henny Widhyastuti .......................................54

[056] Cloning of Haloacid Dehalogenase Gene from Bacillus cereus

Local Strain with the Addition of Restriction Sites
Idris and Enny Ratnaningsih* ...........................................................55

[057] Effect of Glycerol as Carbon Source for Biosurfactant

Production by Halophilic Bacteria Pseudomonas stutzeri BK-
AB12
Monica Putri and Rukman Hertadi* ..................................................56

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 International Symposium on Applied Chemistry 2015
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[058] Synthesis and Characterization of Carbon Nanospheres

Using Cooking Palm Oil as Natural Precursors onto Activated
Carbon Support
a a a
Hans Kristianto , Cahyadi Dwi Putra , Arenst Andreas Arie *, Martin
b b
Halim , and Joong Kee Lee ..................................................57

[059] The Preliminary Study of The Dye Extraction from The

Avocado Seed Using Ultrasonic Assisted Extraction
a a a
Ariestya Arlene A *, Anastasia Prima K , Lidya Utama , Airin
a
Anggraini S ......................................................................................58

[060] Isolation and Characterization of Organic-Solvent Stable

Protease Isolated by Pseudomonas stutzeri BK AB-12
Dian Siti S, Ihsanawati, Rukman Hertadi* .......................................59

[061] Performance of M-Silicate and M-Polystyrene Sulfonate in

Extraction of Carotenoids From Crude Palm Oil
a a a
Nimpan Bangun *, Seribima Sembiring , Nabila Karina Putri ,
b
Justaman Arifin Karo Karo ..............................................................60

[062] Docking and Molecular Dynamics Simulation of Carbonic

Anhydrase II Inhibitors from Phenolic and Flavonoid Group
a b b a
Aditama, R. , Mujahidin, D. , Maolana Syah, Y. ,Hertadi R. * ........61

[063] Esterification of Free Fatty Acid on Palm Fatty Acid

Distillate using Activated Carbon Catalysts Generated from
Coconut Shell
a,b b c b,d
Arif Hidayat , Rochmadi , Karna Wijaya , Arief Budiman ...........62

[064] Antibacterial and Antioxidant Activity Evaluation of 1,3-

Diaryl-prop-2-en-1-one Derivatives
a a a
Melanny Ika Sulistyowaty *, Kholis Amalia Nofianti , Juni Ekowati ,
a a a
Galih Satrio Putra , Tri Widiandani , Tutuk Budiati .........63

[066] Characteristics and Bond Performance of Wood Adhesive

Made from Natural Rubber Latex and Alkaline Pretreatment
Lignin

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a a a
Euis Hermiati *, M. Adly Rahandi Lubis , Lucky Risanto , R. Permana
a b
Budi Laksana , Lukmanul Hakim Zaini ............................64

[067] Alpha Glucosidase Inhibitory Activity of Aqueous Extract of

Cymbopogon citratus, Musa paradisiaca, and Allium cepa L.
var.Aggregatum
a b a
Gregorius Herdiazto Adiyoga , Irvan Kartawiria , FilianaSantoso , and
a
Maria Dewi Puspitasari Tirtaningtyas Gunawan-Puteri *………….............65

[069] Effects of Ionic Liquid as Phase Modifier in PMMA/ENR 50

Blend Electrolyte
a, a
Lina Nadzirah Salahuddin *, Famiza Abdul Latif ............................67

[070] Production of Human Milk Fat Replacement Rich of 1,3 dioleoyl-

2-palmitoilglycerol From Enzymatic Interesterification Tripalmitin,
Ethyl Oleate And Mixture of VCO, Soybean Oil And Fish Oil
Sadiah Djajasoepena*, O. Suprijana, Saadah Diana R., Uji Pratomo,
and Sisca Puspitasari .......................................................68

[071] Structural Analysis of Halophilic Lipases Isolated From

Bledug Kuwu Mud Crater, Purwodadi, Indonesia: a Structural
Bioinformatics study
a b a
Mukhammad Asy’ari , Pingkan Aditiawati , Akhmaloka and Rukman
a
Hertadi * ............................................................................69

[072] Study of Softening Hard Water Using Pistacia Vera Shell As

Adsorbent for Calcium And Magnesium Removal
Uji Pratomo*, Anni Anggraeni, Rubianto A. Lubis and
Allyn Pramudya ................................................................................70

[073] Aqueous-Methanol Extract of Gracilaria verrucosa Induces

Cytochrome c Release From Mitochondria
a a
Mona Fitria , Fida Madayanti Warganegara * ..................................71

[074] Microwave-assisted Fischer indole synthesis of 1,2,3,4-

tetrahydrocarbazole using pyridinium-based ionic liquids
a, a
Ramon M. Eduque, Jr. * and Evelyn C. Creencia ..........................72
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(ISAC 2015)

[075] Characterization of Iron Metal Corrosion in Liquid Smoke

Coagulant
Afrizal Vachlepi1* dan Didin Suwardin1 ...........................................73

[076] Potential of Oil Palm Empty Fruit Bunch (EFB) as Media for
Oyster Mushroom, Pleurotus ostreatus Cultivation
a b
Lin Marlina *, Setiarti Sukotjo ..........................................................74

[077] Impact Strength and Thermal Degradation of Waste

Polypropylene (wPP)/Oil Palm Empty Fruit Bunch (OPEFB)
Composites: Effect of Maleic Anhydride -g-polypropylene (MAPP)
Addition
a a a
Halimatuddahliana Nasution *, Setiaty Pandia , Maulida , Mersi
a
Suriani Sinaga .................................................................................75

[078] Stability Study of Betacyanin Extract from Red Dragon Fruit

(Hylocereus polyrhizus) Peels
a b
Sri Priatni *, Aulia Pradita ...............................................................76

[079] Evaluation of Radiocesium Sorption in Some Materials as Safety

Barrier System of Radioactive Waste Disposal Demonstration Facility
a b b
Budi Setiawan , Elfira Mayasari , Shely Meidhika .........................77

[080] Identification of LL and meso-diaminopimelic acid (DAP) of

Actinomycete strains and Its Secondary Metabolite Production
Desak Gede Sri Andayani, Agustine S., Yetti Mulyati I., Puspa Dewi
L., Hakiki M. .............................................................................78

[081] Encapsulation of Gluten

a b c
Husniati *, Etik Mardliyati , Nofa Mardia Ningsih Kaswati ...............79

[082] Comparison between GC-TCD and GC-FID for the

determination of propane in gas mixture
a a a
Harry Budiman , Nuryatini , Oman Zuas .........................................80

[086] Synthesis of Asymmetric Curcumin analogues from

Cullilawan Oil using Conventional and Microwave Method
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a,c b
Imanuel Berly Delvis Kapelle , Tun Tedja Irawadi , Meika Syahbana
a a b
Rusli , Djumali Mangunwidjaja , Zainal Alim Mas’ud …………..81

[087] Aluminum based composite by Novelty Process: Repetitive

Press Roll Bonding (RPRB)
a,b a a
Agus Pramono *, Lauri Kollo , Renno Veinthal .............................82

[088] Determination of crystallization and melting behaviour of

poly-lactic acid and polypropylene blends as a food packaging
materials by Differential Scanning Calorimeter
a
Achmad Hanafi Setiawan ...............................................................83

[089] Synthesis of 1,4-butanediol monooleate and 1,4-butanediol, 9-

hydroxy-10-methoxy-monostearate from Palm Oil as Modifier of
Epoxy Resin for Coating
Evi Triwulandari, Muhammad Ghozali, and Yenny Meliana ............84

[090] Bioethanol production from glucose by thermophilic

microbes from Ciater hot springs
Takiyah Salim*, Lia Ratnawati, Wawan Agustina, Sriharti ...............85

[091] Measurement and Prediction of Condensate Gas Hydrates

Formation Conditions – A Study Case
2 1 2
Mimoun Hadj ,Samer Said , Otmanine Ghazi and Belloum
1
Mohamed ........................................................................................86

[094] The Potential of Energy Efficiency on Recycle Paper

Bioprocess
1,a 1,b 1,b
Rina Masriani *, Taufan Hidayat , Nina Elyani , Hendro
1,b 2,c
Risdianto and Zeily Nurachman .................................................88

[095] Comparison of Antiglycation and Antioxidant Potentials

and Total Phenolic Contents of Decoctions from Antidiabetic
Plants
a b a
P.R.D. Perera , S. Ekanayake *, K.K.D.S. Ranaweera ..................89

xxxi
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(ISAC 2015)

[096] Polymerization of Blending Cholesteryl Acrylate and Methyl

Phenyl Benzoyl Acrylate
1 2 2 3
Afrizal *, Muhammad Hikam , Bambang Soegiyono , Asep Riswoko ......90

[097] Hydrogenation of Biomass-derived Furfural over Highly

Dispersed-Aluminium Hydroxide Supported Ni-Sn(3.0) Alloy
Catalysts
a
Rodiansono *, Maria Dewi Astuti, Uripto Trisno Santoso, Shogo
b
Shimazu ..........................................................................................91

[098] The Initial Study of Polyaniline with Manganese Oxides for

Electrochemical Capacitors
a b b
Purnama Ningsih *, Clovia Z. Holdsworth and Scott W. Donne ...92

[099] Isolation of thermophilic microbes resistant to ethanol from

Ciater hot springs, Subang-West Java
Lia Ratnawati*, Takiyah Salim, Wawan Agustina, Sriharti ...............93

[100] Cracking Callophyllum innophyllum L.Oil to Bio-gasoline by

Micro-porous based Zeolite and Al2O3 Catalysts
a a a a
Savitri *, R. Effendi , G. Primahana , S. Tursiloadi .........................94

[101] Development of Green Nickel-Based Zeolite Catalysts for

Citronella Oil Conversion to Isopulegol
a b a a
S. Tursiloadi , A. A.Litiaz , R. Pertiwi , I. B. Adilina and K.C.
a
Sembiring * .......................................................................................95

[103] In vitro Anthelmintic Activity Evaluation of the Aerial Part of

Ruellia tuberosa Linn. Against Eudrilus eugeniae
a a
Kirstin Rhys S. Pueblos *, Jeanne Phyre B. Lagare , Radyn Vanessa
a a
Phaz P. Tapales , and Mark Tristan J. Quimque .............96

[104] Green Synthesis of Silver Nanoparticles using Rizhome

Extract of Galangal, Alpinia galanga
a b
Alyza A. A. Azmi *, Norhidayah M. Ahyat .......................................97

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[106] Structural and Morphological Analyses of Chitin and its

Complex upon Manganese Ion Adsorption
a a,
Meg Ina S. Acas and Mark Tristan J. Quimque * ...........................98

[107] Study on the Nitration Reaction of N,N-diphenylamine

Compounds and Its Characterization as Organic Light Emitting
Diode (OLED) Material
a, a a
Haryanto *, Deana Wahyuningrum , Anita Alni ............................100

[108] XPS Analysis and Antibacterial Assay of Novobiocin

Coating
a b c
Hardi Ys * , Susan Semple , Hans J. Griesser ............................101

[109] Functionalization of MCM-41 and SBA-15 Bottom Ash with

APTES
a a
Nurul Barakah Ab Rahman , Hamizah Md Rasid , Hashazirah
a a
Mohammad Hassan , Mohammad Noor Jalil * ..............................102

[111] Regenerating NADH enhances ethanol production in

aerobic culture of recombinant Escherichia coli
a b, b
Fithriani , Prayoga Suryadarma *, Djumali Mangunwidjaja .........103

[112] Multistages preparation for microfibrillated celluloses

based on Arenga Pinnata “ijuk” fiber
a,b, b b
Evana Yuanita *, Juniko Nur Pratama , Mochamad Chalid .......104

[113] Structural properties of Ni/γ−Al2O3 and Cu/γ−Al2O3 catalyst

and its application for hydrogenation of furfurylidene acetone
Siti Mariyah Ulfa*, Inra Sari, Chairulita Putri Kusumaningsih, M. Farid
Rahman ................................................................................105

[114] Effect of CuO nanoparticle on mechanical and thermal

properties of palm oil based alkyd/epoxy resin blend
a,b a, b
Huei Ruey Ong , Md. Maksudur Rahman Khan *, Ridzuan Ramli ,
a
Rosli Mohd Yunus ............................................................106

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[115] Enzymatic Conversion of Glycerol to Glyceric Acid with

Immobilised Laccase in Na-Alginate Matrix
Chi Shein Hong, Sim Yee Chin, Chin Kui Cheng, Mahmud Mohd
Sabri,and Gek Kee Chua* ....................................................107

[116] MnO2/CNT as ORR Electrocatalyst in Air-Cathode Microbial

Fuel Cells
a a b
Chee Wai Woon , Huei Ruey Ong , Kwok Feng Chong , Kar Min
a a,
Chan , Md. Maksudur Rahman Khan * ...................................108

[118] Proficiency testing and laboratory performance in the field

of pH measurement: An Indonesian experiment
a a a a
Nuryatini Hamim *, Sujarwo , Ayu Hindayani , Christin Elishian ,
a
Oman Zuas ...................................................................109

[119] Enzymatic Extract Fractionation of (Cymbopogon

winterianus Jowitt) Citronella through Supercritical Carbon
dioxide (SC-CO2)
a a
Lloyd Arvin M. Malaluan , Roberto M. Malaluan ...........................110

[120] Inhibition of Microbial Growth by Nano-chitosan for Fresh

Tilapia (Oreochromis sp) Preservation
a b
Budhijanto Budhijanto , Prihati Sih Nugraheni , and Wiratni
a
Budhijanto * ....................................................................................111

[122] Optimization of ketoprofen-loaded chitosan nanoparticle

ultrasonication process
a b c
Purwantiningsih Sugita , Laksmi Ambarsari , and Lidiniyah ........112

[123] Potency of Rhizopora spp. Extracts as Antioxidant and

Inhibitor of Acetylcholinesterase
a,b a,b a
Wulan T. Wahyuni , Latifah K. Darusman , Nurzakiah K. Surya …..113

[124] Bioactivity of Polysaccharide from Gracilaria verrucosa as

α-Glucosidase Inhibitor

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a a a
Vienna Saraswaty *, Tjandrawati Mozef , Chandra Risdian , Abdullah
b
Rasyid ............................................................................114

[125] Synthesis of 2-Allylphenyl Cinnamate and Brine Shrimp

Lethality Test Activity Evaluation
a a a
Gian Primahana *, Teni Ernawati , Puspa Dewi N. L , Indah Dewijanti
a a a
Dwiyatmi , Akhmad Darmawan , Muhammad Hanafi ...115

[126] Iron Carboyl Thiourea Hybrid Material Based onFunctionalised

Silica from Rice Husk Ash for Oxidation ofLimonene
a
Nadiah Ameram, Farook Adam * ..................................................116

[132] The stability of Poly(ethersulfone) membranes treated in

hot water and hypochlorite solution
a a a b
Nasrul Arahman *, Nursidik , Mukramah , Sharvina Maulidayanti , Ayu
c
Octria Putri .....................................................117

[133] Carbonylation of Methanol with CO in Presence Potassium

Methoxide Catalyst
a,b b b
Hendriyana , Subagjo , Herri Susanto * ....................................118

[134] Effect of Regeneration Temperature on Particle

Characteristics and Extent of Regeneration of Saturated SO2-
Adsorption of CuO/Ag-Al2O3 Adsorbent
a a a
David Bahrin , Subagjo , Herri Susanto * .....................................119

[135] Novel Synthesis, Antimicrobial, Antioxidant and Antitumor

Evaluation of Imidazolyl Pyrazolopyridine and Imidazolyl
Pyrazoloquinoxaline Derivatives
Mohamed A. El-Borai*, Mohamed E. Sadek, Mai M.El-Keiy .........120

xxxv
 International Symposium on Applied Chemistry
2015 (ISAC 2015)

Nanostructured silicon in nanomedicine

Nicolas H. Voelcker*
Future Industries Institute, University of South Australia, Australia,

Abstract
This talk will explore the application of nanostructured silicon
including porous silicon and silicon nanowires in localised drug
delivery, optical and electrochemical biosensors and tissue
engineering. I will first introduce nanostructured silicon material
properties in the context of biomaterials. This will be followed by an
overview of fabrication and characterisation aspects, including
describing strategies for nano- and microscale patterning and
gradient formation. Then I will give some examples of where porous
silicon has been used in the design of biosensors that incorporate
signal amplification mechanisms. In the following, I will highlight
applications of porous silicon nanoparticles for targeted drug delivery
and of silicon nanowires for gene delivery. The talk will end with an
example of porous silicon for the use as a biodegradable biomaterial
in ocular tissue engineering, followed by a summary and
conclusions.

* Corresponding author : nico.voelcker@unisa.edu.au

xxxvi
 International Symposium on Applied Chemistry
2015 (ISAC 2015)

Ni based alloy catalysts for

chemoselective hydrogenation of
unsaturated carbonyls
SHIMAZU, Shogo*
Department of Applied Chemistry and Biotechnology, Chiba University,
Japan

Abstract
Inexpensive Ni based alloy (Ni-M, M = Sn, Fe) catalysts (both bulk
and supported) were prepared by a simple hydrothermal method[1-
3]. Ni-Sn alloy catalyst showed high selectivity in hydrogenation of
various unsaturated carbonyl compounds, producing unsaturated
alcohols almost exclusively. Ni-Sn alloy catalyst supported on TiO2
showed a remarkable reduction of the reaction temperature from 453
to 383 K with high chemoselectivity. Both Ni3Sn2 and Ni3Sn alloy
phases were found to be responsible in the enhancement of the
chemoselectivity and reusable without any significant loss of
selectivity. In the case of Ni-Fe alloy, addition of Fe to Ni increased
the selectivity of furfuryl alcohol (FFalc) from furfural (FFald). Ni/Fe
ratio = 2 showed the highest performance compared with other
ratios. Hydrogen treatment of Ni-Fe (2:1) at high temperature was
the crucial step for obtaining Ni-Fe alloy (JCPDS-38-419) and
increasing the catalytic performance. Ni-Fe (2:1) H2-treated at 573 K
(HT-573) demonstrated that 100% FFald conversion was achieved at
1 MPa of initial H2 and 423 K with over 90% selectivity of FFalc for
60 min. Addition of TiO2 as a support with 25 wt% of Ni-Fe alloy
showed 100% conversion and 91% FFalc selectivity only for 30 min.
No significant loss of the catalytic performance was observed for Ni-
Fe (2:1) HT-573 after 4 times running. The results indicate high
stability and recyclability.

Key words: Chemoselective hydrogenation; Nickel based alloy

catalysts; Unsaturated alcohols synthesis; Furfural

*Corresponding author; e-mail: shimazu@faculty.chiba-u.jp

xxxvii
 International Symposium on Applied Chemistry
2015 (ISAC 2015)

Biorefinery – Opportunities for

sustainable energy, environment and
economic growth

Abdul Wahab Mohammad, PhD

Centre for Sustainable Process Technology, Faculty of Engineering and Built
Environment, Universiti Kebangsaan Malaysia

Abstract
Biorefinery may be defined as an integrated multifunctional plants
that uses biomass as a diverse source of raw materials for the
sustainable generation of a spectrum of different intermediates and
products (chemicals, materials, bioenergy/biofuels). Malaysia and
Indonesia, with a rich source of biomass from the oil palm, rice fields,
as well as other crops such as sago, has immense potential to
embark on biorefineries that can lead towards further economic
growth. Most of this biomass can be considered as lignocellulosic
biomass (LCB) which consists of cellulose, hemicellulose and lignin.
These LCB is a promising feedstock mainly because of its low cost,
abundant availability and low environmental impacts.
Commercialization of fine chemicals from LCB will create local job
markets and improve local economic development [1-3]. The
Malaysian National Biomass Strategy (NBS) [4] has identified that
the know-how to convert lignocellulosic biomass through biochemical
and thermochemical means into sugars and subsequent high value
products will allow Malaysia to tap into the high-value opportunities in
fine chemicals. In this talk, the author will present two cases of
possible biorefinery concept in Malaysia and how such biorefineries
may provide the solution for energy and environmental sustainability
as well as providing impetus for economic growth in Malaysia. The
first case utilizes the existing biomass (oil palm fronds or empty fruit
bunch) as feed materials while the second case considers palm oil
mill effluent (POME) as the feed stream. Both concepts are currently
being studied. Preliminary results will also be presented.

*Corresponding author; e-mail: wahabm@eng.ukm.my

xxxviii
 International Symposium on Applied Chemistry
2015 (ISAC 2015)

Separation of toxic metal ions from other

metal ions (environmental chemistry)

Yu Komatsu
Environmental Research Institute, Kanazawa Institute of Technology

Abstract:
The atomic plant accident was hard in Tohoku area Japan after the
big earthquake in March 11, 2011. We should clean up the wastes
including radioactive cesium ions. First, we made the new ion
exchanger to adsorb these dangerous elements. The hydrogen tetra
titanate hydrate fibers have ion-exchangeable protons which can ion
exchange with radioactive cesium ions. Cesium ions have strong ion
exchange ability compare to other alkali metal ions, such as lithium
ions, sodium ions, potassium ions and rubidium ions. After cesium
ions were ion exchanged with hydrogen ions which exist in the
hydrogen tetra titanate hydrate fibers, the fibers were heat-treated
with titanium dioxide. The heat-treated titanate fibers keep the
cesium ions almost completely.

key words: ion-echanger, dihydrogentetratitanate hydrated,

radioactive waste, cesium

*Corresponding author; e-mail: ykomatsu@neptune.kanazawa-

it.ac.jp

xxxix
 International Symposium on Applied Chemistry
2015 (ISAC 2015)

Hierarchical structure control of organic

rganic
and inorganic polymer materials
aterials

Yuya Oaki
Department of Applied Chemistry, Faculty of Science and Technology, Keio
University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522,
8522, Japan
email: oakiyuya@applc.keio.ac.jp

Abstract

Morphology control of organic and inorganic polymer

materials in multiple length scales is a central importance for
development of functional materials. The current presentation

shows morphology control of organic polymer materials from

nanoscopic to macroscopic
scopic scales based on growth control of
crystals. We recently developed these approaches based on
the bioinspired approaches. In general, molecular design of
monomers and their precise polymerization have been well

xl
 International Symposium on Applied Chemistry
2015 (ISAC 2015)

studied in previous works. However, it is not easy to achieve

simultaneous synthesis and morphogenesis of organic and
inorganic polymer materials. Here we focused on conductive
polymers as an organic polymer and inorganic monolayers as
an inorganic polymer. Our recent studies show that
interspace, surface, and structure of crystals facilitate
hierarchical morphology control of organic polymer
(1–10)
materials. The improved and emergent properties were
observed on the resultant polymer materials. If the
morphologies of layered inorganic compounds are controlled,
the lateral size and surface chemistry of the monolayered
(11–13)
materials as an inorganic polymer can be controlled.
These crystal-induced morphology control can be applied to a
variety of organic and inorganic polymer materials.

References:(1) Y. Oaki, M. Kijima, H. Imai, J. Am. Chem. Soc., 2011, 133,

8594. (2) M. Kijima, Y. Oaki, Y. Munekawa and H. Imai, Chem. Eur. J.,2013,
19, 2284. (3) Y. Munekawa, Y. Oaki and H. Imai, Langmuir, 2014, 30, 3236.
(4) Y. Munekawa, Y. Oaki, K. Sato and H. Imai, Nanoscale, 2015, 7, 3466. (5)
K. Sato, Y. Oaki, and H. Imai, Chem. Commun., 2015, 51, 7919. (6) K.
Kuwabara, Y. Oaki, R. Muramatsu and H. Imai, Chem. Commun., 2015, 51,
9698. (7) Y. Oaki, R. Muramatsu, H. Imai, Polym. J. 2015, 47, 183. (8) R.
Muramatsu, Y. Oaki, K. Kuwabara, K. Hayashi and H. Imai, Chem. Commun.,
2014, 50, 11840. (9) M. Okaniwa, Y. Oaki, S. Kaneko, K. Ishida, H. Maki, H.
Imai, Chem. Mater. 201527, 2677. (10) M. Okaniwa, Y. Oaki, H. Imai, Bull.
Chem. Soc. Jpn. 2015, in press. (11) K. Nakamura, Y. Oaki and H. Imai, J.
Am. Chem. Soc., 2013, 135, 4501. (12) M. Honda, Y. Oaki and H. Imai,
Chem. Mater., 2014, 26, 3579. (13) M. Honda, Y. Oaki and H. Imai, Chem.
Commun., 2015, 51, 10046.

xli
 001
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Development of semi empirical equation of

limiting current for Lead (Pb) Magneto
electrodeposition

Sudibyoa and N. Azizb,*

a
Indonesian Institute of Science, Division of Mineral Processing, Lampung 35361, Indonesia
b
School of Chemical Engineering, Universiti Sains Malaysia, Pulau Pinang 14300, Malaysia

Abstract
An electrodeposition process placed in a magnetic field or magneto
electrodeposition (MED) cause the increment of limiting current and the change of
electrodeposits become more compact. This method is important process to
produce of nano materials and micro electronics devices. Knowing the limiting
current in electrodeposition process is very important because the optimum mass
transport achieve at this condition. This limiting current under magnetic influence
(iB) can be predict accurately using semi empirical equation, which have
parameters that influence on mass transport phenomena on MED. The semi
empirical expression of iB for lead (Pb) MED was established for our experimental
setup.

Keywords: limiting current; lead; magnetoelectrodeposition, equation

1
 002
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Mixed linker metal organic framework as an

efficient and reusable catalyst for Suzuki Miyaura
coupling reaction

Siti Nurhazlin Jaluddina,*, Karimah Kassimb,Wan Nazihah Wan

Ibrahima
a
Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, 40450, Malaysia
b
Institute of Sciences, Universiti Teknologi MARA, Shah Alam, 40450, Malaysia

Abstract
Series of metal organic frameworks from nitrogen based ligand were synthesized as
efficient and reusable catalyst via mixed linker methods. The thermogravimetric
study of the mixed linker metal organic framework (MIXMOF) reveals that the
complexes are potential thermally stable materials. The palladium supported
catalysts exhibits high catalytic activity toward the Suzuki-Miyaura cross coupling
reaction and can be reused several times without any visible loss of activity even
after five consecutive times.

Keywords: Mixed linker metal organic framework, heterogeneous catalyst, Suzuki

Miyaura coupling reaction, reusable catalyst

2
 003
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Voltammetric determination of Cr(VI) using gold

nanoparticles-modified glassy carbon electrode

Santhy Wyantuti, Safri Ishmayana, Yeni Wahyuni Hartati

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas
Padjadjaran, Jln. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang, West Java,45363,
Indonesia

Abstract
Gold nanoparticles-modified glassy carbon electrode was utilized in the present
study to determine trace amount of Cr(VI). The steps employed in the present study
were fabrication of gold nanoparticles colloids, modification of glassy carbon
electrode using the gold nanoparticle colloids, and voltammetric determination of
Cr(VI) using the modified electrode. The results of the present study indicate that
290 µL of 0.1 M NaBH4 was required to form gold nanoparticles colloids. Self
assembly process was found to give better gold nanoparticles binding onto glassy
carbon electrode compared to adsorption process as indicated by more gold
nanoparticles attached onto the glassy carbon electrode. Modification of the glassy
carbon electrode using gold nanoparticles increase sensitivity as indicated by good
limit of detection (2.38 ng/L) with an acceptable linearity range within 0.050 – 0.250
µg/L. The correlation coefficient and precision of the method were 0.9948 and
99.14%, respectively.
.
Keywords: chromium(VI); Voltammetry; glassy carbon electrode; gold
nanoparticles

3
 004
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Utilization of distillation residue of 2nd generation

bioethanol for fine chemicals production

Sabar P.Simanungkalita,*, Dieni Mansura, Nino Rinaldia, Robert

R.Widjayaa
a
Research Center for Chemistry – Indonesian Institute of Sciences, Kawasan PUSPIPTEK –
Serpong, Tangerang Selatan 15314, Indonesia

Abstract
Distillation residue was waste containing huge amount of water. However, it could
not through away to environment due to water pollution. Therefore, some
treatments were required. Distillation residue of 2nd generation bioethanol based
palm oil empty fruit bunch was hydrothermal liquefied to increase its added value.
Process hydrothermal liquefaction was carried out under variation of temperature
and reaction time, in an autoclave under inert atmosphere. The product consisted of
tar dissolved in water, char and gas. The solid conversion, the tar yields and
chemical compounds yields increased by the increasing the reaction temperature.
The reaction time did not give significant effect to the conversion and the tar yields
(wt %). However, reaction time gave a significant effect to typical chemical
compounds in the tar. The typical chemical compounds identified in the tar were
acetic acid, phenol, phenol,-2-methoxy and 2-pyrrolidinone. Acetic acid as a fine
chemical derived from the distillation residue was recovered as 84% mol after
reaction at 325°C for 60 min.

Keywords: Distillation residue, 2nd generation bioethanol, hydrothermal

liquefaction, fine chemicals.

4
 005
International Symposium on Applied Chemistry 2015
(ISAC 2015)

The Influence of Deproteinisation in the

Morphology of Natural Rubber Latex Particles and
Subsequent Film Formation

Norhanifah M.Y.a,*, Nurulhuda. Ab and Asrul. Mc

a,b, c
Technology and Engineering Division, Malaysian Rubber Board, 47000 Sungai Buloh,
Selangor, Malaysia

Abstract
In latex products manufacturing, protein has always been a problem due to the
prevalence of latex protein allergy. Thus deproteinisation has been introduced to
eliminate or reduce rubber latex protein content. However, the investigation on
particles size and arrangement of particles in the rubber latex state before and after
deproteinisation process has not been thoroughly explored. In this study, the
particles size, arrangement and morphology of the film formed from modified
latexes were monitored using Environmental Scanning Electron Microscopy
(ESEM) and Field Emission Scanning Electron Microscopy (FESEM) for various
latexes treated with either urea or sodium dodecyl sulphate and combination of both
as deproteinisation agent. The size of latex particles changed after deproteinisation
and the arrangement of particles when films are formed revealed interesting
packing occurrence between the deproteinised latexes. These occurrences were
shown to be associated to chemicals or surfactant used in the deproteinisation. The
use of urea in combination with sodium dodecyl sulphate in the deproteinisation
contributed to good latex particles packing during film formation attributed to the
appearance of soft shell latex particles. Clearly, the latex particles and film
morphologies is profoundly influenced by the type of deproteinisation agents used.

Keywords: Natural rubber Latex Particle; Film formation, Deproteinisation;

Environmental Scanning Electron Microscopy (ESEM); Field Emission
Scanning Electron Microscopy (FESEM)

5
 006
International Symposium on Applied Chemistry 2015
(ISAC 2015)

RECOVERY OF INULIN FIBER FROM LOCAL RED

DAHLIA (Dahlia sp. L) TUBER THROUGH
ENZYMATIC HYDROLYSIS USING Aspergillus sp.-
CBS5AND Bacillus sp.-CBS6 FOR FUNCTIONAL
FOOD

Agustine Susilowati, Aspiyanto, Hakiki Melanie, Jeti Mulyati

and Yati Maryati
Research Centre for Chemistry, Indonesian Institute of Sciences,
PUSPIPTEK, Serpong 15314, Banten, Indonesia

Abstract
Enzymatic hydrolysis of local red dahlia (Dahlia sp. L.) tuber from Sukabumi (West
Java), could generate Soluble Dietary Fiber (SDF) and Insoluble Dietary Fiber
(IDF), which have important role in rate of cholesterol synthesis. Hydrolysis was
carried out by using 2 kinds of crude inulinase enzyme from Aspergillus sp.-CBS5
and Bacillus sp.-CBS6 at concentration of 20, 40 and 60 % (v/w total sugar), pH 5
and 30 °C for 24, 48, 56, 72, 96 and 120 hours for inulin hydrolysate, and result of
hydrolysis in step I by commercial β-Amylase enzyme. The result showed that
hydrolysis time would increase SDF and reducing sugar, decrease total solids and
IDF, and fluctuate total sugar. Treatment of hydrolysis process affected on each
specific component. Based on the highest SDF, process optimization was reached
by using Bacillus sp.-CBS6 inulinase enzyme with concentration of 20 % (v/w total
sugar) for 120 hours and yielded SDF of 71.752 % (dry weight). In this condition, it
is occurred an increase of SDF in inulin hydrolysate 37.4 % when compared to
before hydrolysis process with composition of IDF and SDF at concentration
relatively of 1 : 6.24.

Keywords: inulin, inulinase enzyme, Aspergillus sp.-CBS5 , Bacillus sp.-CBS6 ,

Soluble Dietary Fiber (SDF), Insoluble Dietary Fiber (IDF).

6
 007
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Cyclic Oxidation of Aluminide Coated Two Phase

α2-Ti3Al/γ-TiAlAlloys at 1000oC

Eddy A. Basukia*, Dita Siti Hajarb, Farhan Rahmana and Djoko

Prajitnoc
a
Department of Metallurgical Engineering, Faculty of Mining and Petroleum Engineering,
Bandung Institute of Technology, Indonesia
b
PT. Antam Tbk, Indonesia
3
Nuclear Technology Center for Materials and Radiometry, National Atomic Agency of
Indonesia

Abstract
Titanium aluminide alloys have been considered as the candidates to substitute
nickle-base superalloys for high temperatureapplications in aircraft turbine engines,
as the alloys has lower density. For such applications, the alloys should exhibits
bothstrength and high temperature oxidation or corrosion. Therefore, external
protective scale of Al2O3 has to be easily developed inthe alloys, and the scale
should resist for cyclic thermal operations. Recently, pack aluminide coatings have
been developed ontwo phase α2-Ti3Al/γ-TiAl alloys of Ti-47Al-2Nb-2Cr-0.5Y-0.5Zr,
to provide TiAl3 based coatings capable in providing externalscale of Al2O3.
Nevertheless, no cyclic oxidation test has been conducted in this system. This
paper reports the cyclic oxidationbehavior of un-coated and pack aluminide coated
o
α2-Ti3Al/γ-TiAl alloys at 1000 C.

Keywords: TiAl alloys; pack aluminide coating; cyclic oxidation.

7
 008
International Symposium on Applied Chemistry 2015
(ISAC 2015)

A new flavonoid derivative as cytotoxic

compound isolated fromethyl acetate extract of
Macaranga gigantifolia Merr. Leaves

Akhmad Darmawan, Megawati, Puspa Dewi N. Lotulung, Sofa

Fajriah, Gian Primahana,and Lia Meiliawati
Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPIPTEK
Serpong, Kota Tangerang Selatan, Banten,15314,Indonesia

Abstract
A new prenylated flavonoid compound 5,7,3',4'-tetrahydroxy-3,6-diprenylflavone (1)
has been isolated from methanol extract ofthe leaves of Macaranga gigantifolia
Merr. Isolation was performed using chromatography methods and their structures
wereelucidated based on spectroscopic data. cytotoxic activity against murine
leukemia P-388 cell line conducted using MTT method.Compound 1 showed strong
cytotoxic activity with IC50 value 6.19 µg/mL.

Keywords: Macaranga gigantifolia; Euphorbiaceae; 5,7,3’,4’-tetrahydroxy-3,6-

diprenylflavon; murine leukemia P-388 cell line.

8
 009
International Symposium on Applied Chemistry 2015
(ISAC 2015)

REDUCING SALT FROM AUTOLYSATE OF

FERMENTED MUNG BEAN (Phaseolus radiatus
L.) USING DIAFILTRATION-NANOFILTRATION (DF-
NF) MODE FOR QUALITY IMPROVEMENT OF
SAVORY FLAVOR PRODUCT

Aspiyanto, Agustine Susilowati and Hakiki Melanie

Research Center for Chemistry, Indonesian Institute of Sciences,
Kawasan PUSPIPTEK, Serpong, South Tangerang – 15314, Indonesia

Abstract
Diafiltration-Nanofiltration (DF-NF) mode of fermented mung beans (Phaseolus
radiatus L.) autolysate by Rhizopus sp.-PL19 and Aspergillus sp.-K3 to recover L-
glutamic acid as savory fraction with salt concentration relating with organoleptic
aspect has been done. DF-NF mode was performed by adding pure water to
autolysate feed in 0/3500, 700/3500, 1400/3500, 2100/3500, and 2800/3500 mL/mL
as Number of diavolume (Nd) of 0, 0.2, 0.4, 0.6, and 0.8 at DF flow rate of 35.66
and 35.5 mL/minute at pump motor frequency of 20 Hz (~ 7.5 L/minute), room
temperature, and pressure of 20 bar for 155.3 and 197.6 minutes, respectively. The
result indicated that DF-NF mode on autolysate-Rhizopus sp.-PL19 and autolysate-
Aspergillus sp.-K3 was able to reduce salt at Nd of 0.2, namely 20 and 4.76 %,
respectively. High Nd gave high salt reduction in retentate. At the optimal Nd (0.2)
was resulted compositions of retentate-Rhizopus sp.-PL19 and retentate-
Aspergillus sp.-K3 with concentrations of salt of 0.106 and 0.53 %, N-amino of 7
and 7 mg/mL, and L-glutamic acid as savory fraction of 0.5063 and 0.8437 % (total
2
protein), respectively, while at permeate gave flux value 70 and 51.23 L/m .hour,
and concentrations of salt 0 and 0.1325 %, N-amino 3.5 and 1.4 mg/mL, and L-
glutamic acid 0.6375 and 0.3188 % (total protein), respectively.

Keywords: Diafiltration-Nanofiltration (DF-NF), Number of Diavolume (Nd), L-

glutamic acid, retentate (concentrate), permeate, salt.

9
 010
International Symposium on Applied Chemistry 2015
(ISAC 2015)

EVALUATION OF CULTURE MEDIA FOR

BUTYROLACTONE I PRODUCTION BY
Aspergillus terreus LS07 AS ANTIDIABETIC

RiznaTriana Dewi*, Hani Mulyani, Puspa D.N. Lotulung and

Nina Artanti
Research Center for Chemistry - Indonesian Institute of Sciences
Kawasan PUSPIPTEK Serpong – Tangerang Selatan, Banten, Indonesia

Abstract
Butyrolactone I (BL-I) has been reported as inhibitor of α-glucosidase and
antioxidant compound isolated from culture broth of Aspergillus terreus. The
purpose of this study was to determine the effect of various liquid media
compositions on production of BL-I from A. terreus LS07. Fermentation was
conducted in four liquid medium: potato dextrose broth (PDB), potato malt peptone
(PMP), malt extract (ME), and Czapek-Dox broth (CDB), which were incubated for
seven days at room temperature, with agitation of 70 rpm. Ethyl acetate (EtOAc)
was used to extract the active metabolite from each broth. Glucosidase inhibitory
activity was measured by the inhibition of yeast α-glucosidase, while antioxidants
measured by testing the total phenol and DPPH free radical scavenger activity. The
results showed that the EtOAc extract on CDB had strong inhibition activity against
the enzyme α-glucosidase and strong antioxidant with IC50 values 6.45 and 24.05
µg/mL, respectively. The CDB extract also showed the highest content of total
phenols (349.66 mg Gallic Acid Equivalent (GAE)/g extract) and BL-I (58.5% w/w).
Based on these results, among the four kinds of medium used, Czapek-Dox broth is
an ideal culture medium of A. terreus LS07 that could be applied for increase
production of BL-I as natural antidiabetic source from terestrial fungi.

Keywords: Butyrolactone I, α-glucosidase inhibitor, antioxidant, Aspergillus terreus

LS07, Czapek-Dox broth

10
 011
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis and Characterization of ZnO

Nanoparticles by Sol-Gel Method with Various
Additives

Sherly Kasuma Warda Ningsih*, Miftahul Khair, Silvi Veronita

Department of Chemistry, Faculty of Mathematics and Natural Sciences

Padang State University, Kampus Air Tawar, Jl. Prof. Dr. Hamka, West Sumatera
25161, Indonesia

Abstract
Preparation of zinc oxide (ZnO) nanoparticle with various additives by sol-gel
process has been studied. ZnO nanoparticles were obtained by using zinc sulfate
heptahydrate as the precursor, ethanol as the solvent. Ethylene glycol and urea
were used as the additives by the addition of potassium hydroxide solution to adjust
o
the pH value. The powders were formed by drying in the temperature of 105 C for
o
60 minutes and after heating at 500 C for ± 1 hour. The products were obtained in
white powders. The synthesized ZnO were characterized by X-ray diffraction (XRD)
and Scanning Electron Microscopy (SEM). The XRD patterns showed ZnO forms
were produced generally in hexagonal structure (wurtzite). Crystallite sizes of ZnO
were estimated by using Scherrer equation. The particle size of ZnO prepared by
using ethylene glycol and urea obtained in the range 18-70 nm and 26-75 nm
respectively. SEM micrograph of ZnO shows agglomeration of hexagonal
nanoparticles and the the distribution size is 0.1-1.0 µm approximately.

Keywords: ZnO nanoparticle; additive; hexagonal; sol-gel; agglomeration

11
 012
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Determination of Assigned Values for Cadmium,

Cobalt and Manganese in Drinking Water
Proficiency Testing

Eka Mardika Handayania*, Isna Komalasari, Rosi Ketrin

Research Center for Chemistry, Indonesian Institute of Sciences (RCChem-LIPI) Kawasan
PUSPIPTEK, Serpong, 15314, Indonesia

Abstract
In proficiency tests the selection of an appropriate assigned value is one of the most
critical points. Determination of the assigned value for proficiency test can be done
in 2 ways: based on reference value and consensus value. The use of such a
consensus value as an assigned value is a commonplace in proficiency testing,
because there is seldom at present a cost effective alternative. However, the
consensus value has a weakness in that it could be significantly different from the
true value due largely inexperienced laboratories. Therefore in this work, the
reference values were used as the assigned values for all analytes of interest. The
reference values in spiked drinking water samples were produced by the Metrology
in Chemistry Laboratory of Research Center for Chemistry - Indonesian Institute of
Sciences (RCChem-LIPI) with traceable to SI units through standard solutions from
the National Institute Standard and Technology, USA. The reference values of
cobalt and manganese were obtained by measurement using two from three
comparable methods of Graphite Furnace-Atomic Absorption Spectrometry (GF-
AAS), Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) and
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) while that for cadmium
was obtained by a primary method of ICP-Isotope Dilution Mass Spectrometry (ICP-
IDMS). The obtained reference values were used to evaluate the laboratory
performance result in a proficiency testing scheme of cadmium, cobalt and
manganese in drinking water which was provided in 2014. All fifty nine laboratories
reported cadmium and cobalt at low concentration levels which were about more
than 82 % showed satisfactory performance results. Similarly, the results of
manganese were also comparable at high level concentrations.

Keywords: proficiency testing; reference value; drinking water

12
 013
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Characterization of Inulin from Local Red Dahlia

(Dahlia sp. L) Tubers by Infrared Spectroscopy

Hakiki Melanie , Agustine Susilowati, Yety M. Iskandar, Puspa

D. Lotulung and Desak G. S. Andayani
Research Center for Chemistry, Indonesian Institute of Sciences,
Kawasan Puspiptek Serpong, Tangerang Selatan 15314, Indonesia

Abstract
Inulin consists of soluble dietary fiber and insoluble dietary fiber, which is
undigested carbohydrate produced by inulin extraction from local red dahlia tuber
(Dahlia sp. L). Extraction of inulin was carried out through gelatinization by heating
at a temperature of 90 ºC for 30 minutes at pH 5 (control) and 10, respectively, then
precipitated with ethanol to obtain inulin and continued with characterization by
infrared spectroscopy (FT-IR). Based on FT-IR spectral analysis, the results showed
-1
characteristic absorption bands of inulin structure, OH stretch (3350 cm ) and
-1
carbonyl (1645 cm ). In addition, four characteristic regions can be distinguished
-1 -1 -1 -1
from 3600 to 2500 cm , 2500 to 1550 cm , 1500 to 900 cm and under 900 cm .
The difference of infrared spectra obtained from each sample of inulin could be
caused by different structure and influence of glucose, sucrose and mannan existed
in inulin and the purification stage during treatment in alkaline condition.

Keywords: inulin; local red dahlia; extraction; soluble dietary fiber; infrared spectral

13
 014
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Chemical Modification of Upgraded Bentonite as

Filler of Natural
Rubber/Organobentonite Composite

Arief Ramadhana*, Mohamad Irfan Fathurrohmana, Bambang

Soegijonob
a
Indonesian Rubber Research Institute, Jl. Salak No 1 Bogor 16151, West Java, Indonesia
b
Department of Physics, Faculty of Mathematics and Natural Sciences, University of
Indonesia, Depok 16424, West Java, Indonesia

Abstract
Bentonite is an abundant natural resource that has great prospects to be used as
filler in natural rubber industry after certainmodification, either through physical or
chemical process. Reduction of mineral impurities in the bentonite was done to form
upgraded bentonite by using hydrocyclone, and chemical modification of upgraded
bentonite to form organobentonite was doneby addition of commercially surfactant.
Natural rubber/organobentonite composites made by melting compound technique
at rollmill. The results showed that the reduction process of mineral impurities in the
bentonite had been able to reduce the content ofimpurities, such as Fe2O3, CaO,
and K2O. Meanwhile, by using XRD dan FTIR analysis, an increase in the dose of
surfactant onupgraded bentonite might increase the organic content and basal
spacing of organobentonite. Low mineral impuritiesorganobentonite was capable of
producing natural rubber/organobentonite composite with faster vulcanization time
and somebetter mechanical properties (such as elongation at break and modulus
100%) than natural rubber/carbon black composite.

Keywords: bentonite, hydrocydone, composite, natural rubber, organobentonite,

surfactant

14
 015
International Symposium on Applied Chemistry 2015
(ISAC 2015)

A novel molecularly imprinted Acrylonitrile-

Butadiene-Styrene (ABS) membrane for
absorption of hippuric acid

Mohamad Raizul Zinalibdinab, Abdul Rahim Yacoba and Mohd

Marsin Sanagia
a
Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, UTM Skudai,
81310 Skudai, Johor, Malaysia.
b
Department of Chemistry, Johor Branch, Jalan Abdul Samad,80100 Johor Bahru, Johor

Abstract
The abundance of electronic waste has now become a major problem. For
sustainability and waste to wealth, the concept of reuse, refurbished and recycled
has been forwarded. In this research the electronic waste ABS plastic was recycled
and molecularly imprinted for fast, sensitive, selective and robust absorption
membrane. The extraction of biological fluid has always being a challenge with the
emulsification of solvent and destruction of sorbent pack. Thus, using the developed
molecularly imprinted polymer membrane (MIP ABS), adsorption of hippuric acid is
studied. For optimization the parameters that influenced the adsorption efficiency
were also investigated. For comparison the non-molecularly imprinted membrane
(NIP ABS) used as blank was also prepared. This study, the standard calibration
curves of 5 mg/L to 40 mg/L of hippuric acid against the MIP and NIP ABS were
2
obtained. The coefficients determination R for MIP ABS were 0.9994 and with the
regression equation of y = 28845x –16638 respectively. This research also found
that pH 6 of the sample solution was the optimized condition with recovery
percentage of 98.56%. hippuric acid bound with the MIP ABS membrane.

Keywords: Molecularly imprinted polymer, hippuric acid, ABS membrane,

Absorption, Sol-gel method.

15
 016
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Alkaline Delignification of Oil Palm Empty Fruit

Bunch using Black Liquor from Pretreatment

Muryantoa*, Eka Triwahyunia, Haznan Abimayua, Agung

Cahyonob, Effendi Tri Cahyonob, Yanni Sudiyania
a
Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan Puspiptek
Serpong, Tangerang Selatan 15314, Indonesia
b
Agricultural Engineering Department, University of Brawijaya, Jl. Veteran Malang, Indonesia

Abstract
Bioethanol is providing one window of potential alternative energy. Lignocellulosic
from oil palm empty fruit bunch (EFB) could be a promising bioethanol raw material
because of abundant resources and it will not interfere food supply. Pretreatment is
a one of the steps in the bioconversion of lignocellulosic material. Pretreatment also
contributes the largest cost in the bioethanol production and produces black liquor
as a wastewater that provides environmental impacts. Black liquor recycling is
expected to increase economic efficiency by reducing the cost of pretreatment and
the amount of wastewater. This experiment used black liquor and its mixture with
NaOH solution as pretreatment solution. The pretreatment process was conducted
o
in a 5-liter reactor at 150 C. The result using only black liquor gave the lowest value
compared to other solutions. The addition of NaOH solution into black liquor caused
increasing the cellulose content and delignification EFB by 57.18% and 51.62%,
respectively. The maximum glucose concentration in the variation of pretreatment
solutions: NaOH 2.5M, black liquor, and black liquor with NaOH solution addition
-1 -1 -1
were 93.80 g l , 52.55g l and 87.05g l , respectively, at a pretreated biomass
loading of approximately 15 % (w/v) with an enzyme dosage of 30 FPU g/dry
biomass.

Keywords: black liquor; EFB, enzymatic hydrolysis, delignification; pretreatment,

recycle, sugar

16
 017
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Bioactivity of Ethyl Acetate Fraction from the

leaves of “Sukun” (Artocarpus altilis (Parkinson)
Fosberg) in Preventing Atherosclerosis

Tjandrawati Mozefa*, Chandra Risdiana, Elin Y. Sukandarb,

Andreanus A. Soemardjib
a
Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Jl. Sangkuriang,
Bandung 40135, Indonesia
b
School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesha 10 Bandung 40132, Indonesia

Abstract
Artocarpus altilis (Parkinson) Fosberg), a tropical tree, belonging to Moraceae family has been
known in folk medicine to have some health benefits in particular for inflammation, infection,
diabetes and even some cardiovascular problems. One of risk factors of some cardiovascular
diseases is hypercholesterolemia. Previous in vitro works showed that ethyl acetate fraction of
A. altilis exhibited cytoprotective effect in human U937 cells treated with oxidized LDL. This
current work aimed to investigate the effect of ethyl acetate (EA) fraction from the leaves of
Sukun, on serum total cholesterol level and on lipid deposition in aorta in vivo. The EA fraction
was administered in 8-weeks-Wistar rats fed with high cholesterol diet for 30 days. The serum
total cholesterol was dosed using enzimatic test and lipid accumulation in aorta was examined
by histology of parafin-blocked aorta stained with hematoxilin-eosin. Antioxidant capacity of
flavonoids contained in EA fraction was examined by 1,1-diphenyl-2- picryl-hydrazyl (DPPH)
method. The result showed that EA fractions at doses 150 and 300 mg/kg BW were able to
lower significantly serum total cholesterol and to prevent lipid accumulation in rat aorta. DPPH
examination of flavonoids which were cyclocommunol, 1-(2,4-dihydroxyphenyl)-3-[8-hydroxy-
2-methyl-2-(4-methyl-3-pentenyl)-2H-1-benzopyran-5-yl]-1-propanone or AC-31 and 2-
geranyl-2′,3,4,4′-tetrahydroxydihydrochalcone or AC-51 showed IC50 value 1548.8±486.8,
275.3±10.0 and 94.1±1.4 µM respectively. It is concluded that the EA fraction is able to lower
serum total cholesterol and to prevent lipid accumulation in rat aorta, therefore, has the
potential medicinal effect to prevent atherosclerosis. Furthermore, its content of flavonoid
especially 2-geranyl-2′,3,4,4′-tetrahydroxydihydrochalcone is suggested to contribute to this
effect by acting as antioxidant.
.
Keywords: Artocarpus altilis, Flavonoid, Atherosclerosis, Cholesterol, Lipid accumulation,
DPPH

17
 018
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Performance Test of Vitamin B6 Test Kit

Candidate
1 1 1
Teuku Beuna Bardant , Anastasia Fitria Devi , Zatil Afrah Athailah ,
1 2 1
Wirasuwasti Nugrahani , Ahmad Latiful Qolbi , Aspiyanto
1
Research Center for Chemistry Indonesian Institute of Science
2
Syarif Hidayatullah Islamic State University

Abstract
Vitamin B6 test kit was designed as an alternative, affordable and user-friendly analysis toolfor small and
medium enterprises which process bananas. Its performance was evaluated twice with 70 respondents and
the measurements considered satisfying when the results were laid in the range of one square deviation
from the real value that determined by spectrophotometer. Performance tests results then compared to pH
measurement using pH indicator, the more familiar test kit that also rely on human ability to distinguish
color. The first performance test to respondents that closely related to chemical measurement got 33
success results and 29 respondents gave satisfying results which was comparable to pH measurement that
gave 31 of 33 satisfying results. The second performance test was conducted by 70 respondents which
involving respondents with background that unrelated to chemical measurement. As much as 34 success
results for both measurements from the related group but only 32 and 27 success measurements for pH
measurement and Vitamin B6 measurement using kit, respectively, from the unrelated group. The second
performance test results for pH measurement did not give significant difference with the second one, 34 of
35 satisfying results from closely related group and 27 of 32 from unrelated group. Second performance test
results for Vitamin B6 was significantly different from the first one since the respondents required to conduct
the measurement twice and the results were reported as average values. Vitamin B6 kit gave 23 of 61
satisfying results with 15 of 34 were from the closely related group and 8 of 27 from the unrelated group.
Data from closely related group from second performance test were further analyzed by considered as two
separated measurements and compared to the results from the first performance test. It was found that
measurement conducted first gave only 2 of 34 satisfying results compared to 22 of 34 from measurement
conducted at second. The results were also recapitulated and analyzed based on background and gender.
Closely related group gave 44.12 %, 29.41%, 26.47% satisfying, query and unsatisfied measurement
results, respectively. These results were better compare to the unrelated group that only gave 29.63%,
25.93% and 44.44%. Female respondents gave 36.36%, 36.36%, 27.27% satisfying, query and unsatisfied
measurement results, respectively, which better than male respondents that gave 41.18%, 5.88% and
52.94%. Vitamin B6 kit had good performance as a test kit candidate but still required many improvements
so the measurement results doesn’t affected by gender, user background and number of conducted
measurement. Better design of color chart was a proposed improvement to reduce the fatigue of eye’s
conical cell that will be conducted in the next study.

Keywords: pyridoxine, banana, measurement deviation, color perception.

18
 019
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Cloning and Sequencing of Haloacid

Dehalogenase Gene from Klebsiella pneumoniae
ITB1

Candra Yulius Tahyaa, Enny Ratnaningsiha*

a
Biochemistry Division, Chemistry Study Program, Faculty of Mathematic and Natural Science,
Institut Teknologi Bandung Jl. Ganeca 10, Bandung 40132, Indonesia

Abstract
Monochloroacetic acid (MCA) production continues to grow every year and it causes
environmental problems. Klebsiella pneumoniae ITB1 is identified to be able to grow on
minimum medium with MCA as a sole of carbon source. This strain breaks down MCA to
produce 2-hydroxyacetic acid and releases chloride ion into the medium, which can be
detected by colorimetric method. Enzyme responsible for this activity is haloacid
dehalogenase. This experiment amplified the haloacid dehalogenase gene of Klebsiella
pneumoniae ITB1 by PCR. The result was 0.69 kb DNA fragment. This fragment was cloned
to pGEM-T easy and then transformed to competent E. coli TOP10 cells. Recombinant
colonies were selected based on ampicillin resistant and β-galactosidase activity.
Recombinant plasmid confirmation was conducted by size screening, re-PCR, and restriction
analysis. The restriction analysis showed positive result as the EcoRI digests of pGEM-HAD
recombinant produced two DNA fragments of 3.15 kb and 0.71 kb. Nucleotide sequence of
hakp1 DNA fragment confirmed that it is a haloacid dehalogenase gene with 99% similarity to
Klebsiella pneumonia. Amino acids sequence deduced by in silico analysis of hakp1 showed
100% sequence similarity with haloacid dehalogenase of multispecies Klebsiella (Accession
No. WP_004179015.1). Conserve residues analysis using ClustalW2 of Hadkp1 protein
compared to another three haloacid dehalogenases i.e. from Burkholderia cepacia MBA4,
Xanthobacter autotrophicus GJ10, and Pseudomonas sp. YL showed 20 conserve residues.
Five of the 20 conserve residues were catalytic residues, which are aspartate (D), threonine
(T), serine (S), lysine (K) and tyrosine (Y). The 3D structure of Hadkp1 protein predicted by in
silico analysis has been conducted by I TESSER. Blastp analysis also showed that Hadkp1
protein of Klebsiella pneumoniae ITB1 has high similarity to enolase-phosphatase of Klebsiella
pneumonia. Enolase-phosphatase is an enzyme that catalyzes dephosphorylation and
enolization of 1-phosphonooxy-2,2-dihydroxy-3-oxo-5-methylthiopentane to 1,2-dihydroxy3-
oxo-5-(methylthio)-pent-1-ene. This enzyme has important role in methionine salvage pathway
of Klebsiella pneumoniae metabolism.

Keywords: Haloacid dehalogenase; Klebsiella pneumoniae; gene cloning; PCR

19
 020
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis and Toxicity Assessments Some para-

methoxy Chalcones Derivatives

Fitra Perdanaa*, Yum Eryantib, Adel Zamrib

a
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Gadjah
Mada, Sekip Utara, Yogyakarta 55281, Indonesia
b
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Riau,
Kampus Binawidya, Pekanbaru 28293, Indonesia

Abstract
Chalcones is a very interesting compounds because it is known to have various of
biological activities such as antimicrobial, antifungal, anticancer, antimalarial,
antioxidant, antitumor, anti-inflammatory and antidepressant. Moreover, natural and
synthetic compounds of chalcones have roles as precursors for other compounds.
Therefore, many chalcones become model structures of target compounds by
researcher. In this research, methoxy chalcones derivatives have been synthesized
using stirrer method and using base catalyst NaOH. The synthesized results
obtained are (E)-3-(4-isoprophylphenyl)-1-(4'-methoxyphenyl)prop-2-en-1-one (1),
(E)-1-(4'-methoxyphenyl)-3-p-tolylprop-2-en-1-one (2) and (E)-3-(3-bromophenyl)-1-
(4'-methoxyphenyl)prop-2-en-1-one (3). The purity of all compounds have been
tested using TLC, melting point test, analytical HPLC. Then they are characterized
1
using UV, FTIR, H-NMR and MS spectroscopy. The toxicity assessments of the
novel compounds were done by Brine Shrimp Lethality Test (BSLT) method. The all
compounds showed very good activity with LC50 value<200 µg/mL.

Keywords: chalcones; toxicity assessments; Brine Shrimp Lethality Test (BSLT)

20
 021
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Activated Carbon from Lignin-BasedBlack Liquor

Coagulated by Polyaluminium Chloride

Feni Amriania, Vera Barliantia, Muryantoa, Ajeng Arum Saria

a
Indonesian Institute of Sciences, Research Center for Chemistry Kawasan PUSPIPTEK,
Serpong 15314, Indonesia

Abstract
Black liquor obtained from the pretreatment process in bioethanol plant was
coagulated by Polyaluminium Chloride (PACl). The optimum sludge was evaluated
by varying PACl dosage. Further, the sludge from coagulation process was
prepared for activated carbon with the physical activation process. The preparation
was carried out with two different methods: single step activation and two-step
activation. In single step activation, dried sludge of black liquor were activated
o
directly using the furnace at 575 C for 3 h. In two-step activation, dried sludge was
o
carbonized at 575 C for 3 h and subsequently activated with raised temperature at
o
725 C for 1 h. Investigated properties of lignin PACl-based activated carbon in
single (LPACl-based AC 1) and two-step activation (LPACl-based AC 2) in the
present study included its surface area and peak component analysis. It was found
that PACl 1% (w/v) dosage addition to black liquor in constant speed 200 rpm for 10
minutes followed by slow stirring process 50 rpm for 2 hours and 24 hours
settlement served optimum coagulation behaviour to deliver precipitation. Surface
area (SBET) of LPACl-based AC 1 was found higher than LPACl-based AC 2. This
preliminary study was conducted to evaluate influence process variable for the
further study of activated carbon production as utilization of black liquor to establish
sustainable recycling process, consequently supported the environmental and
practical value.

Keywords : Black liquor, activated carbon, PAC, carbonization, physical activation

21
 023
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Optimization of Saccharification and

Fermentation Process in Bioethanol Production
from Oil Palm Fronds

Eka Triwahyunia*, Sri Hariyantib, Deliana Dahnuma,

Muhammad Nurdinb, Haznan Abimanyua
a
Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan Puspiptek
Serpong, Tangerang Selatan 15314, Indonesia
b
Faculty of Mathematic and Science, University of Halu Oleo, Kendari, Sulawesi Tenggara
93232, Indonesia

Abstract
Oil Palm Frond (OPF) is one of lignocellulosic biomass, which can be utilized as
raw material for bioethanol production. Bioethanol is produced as alternative energy
to substitute gasoline. There are four steps in bioethanol production from OPF, i.e
pretreatement, saccharification, fermentation and purification process. In this study,
optimization of saccharification and fermentation process for OPF was investigated.
Two methods and the variations of enzyme concentration were carried out in the
saccharification and fermentation process. Separate hydrolysis and fermentation
process (SHF) and simultaneous saccharification and fermentation process (SSF)
were conducted to produce ethanol optimally. Variations of enzyme concentration
used in this process were 10, 20, 30 and 40 FPU/g substrate. The result shows that
the highest ethanol concentration can be obtained in SSF process with 30 FPU/g
substrate of enzyme concentration. The process produced 59.20 g/L ethanol
(95.95% yield ethanol) at 96 h of SSF process.
.
Keywords: Bioethanol, Oil Palm Frond, Separate Hydrolysis and Fermentation,
Simultaneous Saccharification and Fermentation

22
 024
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of Extraction Method on Total Flavonoid,

Total Phenolic Content, Antioxidant and
Antibacteria Activity of Zingiber officinale
Rhyzome

Rina Andriyania*, Thelma A. Budianaa, Sri Pudjirahartia

a
Functional Foods Division, Research Center for Chemistry, Indonesian Institute of Sciences,
Puspiptek Serpong, 15314, Indonesia

Abstract
This research aims to compare between decocta and maceration method using
water and ethanol as a solvent on total flavonoid, total phenolic content, antioxidant
and antibacteria activity of Zingiber officinale rhyzome. The Rhyzome was dryed by
oven method. Total flavonoid, total phenolic content and antioxidant activity were
assay by spectrophotometric method. The antibacteria activity was determined by
difussion agar method against Bacillus subtilis (BS) and Escherichia colli InaCC B5
(EC). Ethanol extract from dried processs for 24 hours have the best result for each
assay.The yields are 286 µg/mL of total flavonoid, 504 µg/mL of total phenolic
content and 5.753 µg/mL IC50 antioxidant activity. It is also positive as antibacteria
against to BS and EC. The inhibition diameter result against BS for 10% and 20% of
samples concentration are 10 and 11.5 mm. Meanwhile, The inhibition diameter
result against EC for 10% and 20% of samples concentration are 7.5 and 8.5 mm

Keywords: total flavanoid; total phenolic content; antioxidant; antibacteria; Zingiber

officinale rhyzome

23
 025
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of electrodeposition reactor type on nickel

recovery from electroplating wastewater

Djaenudina, Diana Rahayuning Wulana, Hari Rom Hariyadia

a
Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu Bandung 40135,
Indonesia

Abstract
Nickel recovery from synthetic electroplating wastewater using electrodeposition
reactor has been investigated. The experiment was conducted in batch system
using two types of electrodeposition reactors, partitioned reactors using water
hyacinth leaf and single chamber reactor, a copper plate cathode size, graphite
anode and applied DC voltage of 5V. The goal of this research was to obtain the
best reactor type for nickel electrodeposition from NiSO4 solution. Factors analyzed
in the study were the change in pH, conductivity, electric current, cathode mass and
nickel concentration in the catholyte. The electrodeposition was carried out during 4
hours. The initial concentration of nickel in the catholyte contained 2,402.19 mg/L.
The results showed that the partitioned reactor using of water hyacinth leaf
performed better than the single chamber reactor. The partitioned reactor using
water hyacinth leaf decreased concentration of nickel in catholyte to 1756.19 mg/L
and had the current efficiency of 88.17% with the specific energy of 5.2 kwh/kg
nickel.

Keywords: Nickel electrodeposition; partitioned reactor; single chamber reactor;

water hyacinth leaf

24
 026
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Extraction and Quantification of Toxic Compound

Mimosine from Leucaena leucocephala Leaves

Zul Ilham*, Hanisjasymah Hamidon, Nurul Amalina Rosji,

Norfadhilah Ramli, Normaniza Osman

Institute of Biological Sciences, Faculty of Science, University of Malaya 50603

Kuala Lumpur, Malaysia

Abstract
The existence of mimosine in Leucaena leucocephala prevents the utilization of its
biomass residues (after energy conversion) as animal feed. In this study, mimosine
quantification in Leucaena leucocephala was carried out by using rapid colorimetric
method. In addition, two different extraction methods which are soxhlet extraction
with either distilled water or ethyl acetate as extraction solvent and digestion
method were used to compare its efficiency in extracting the mimosine from
Leucaena leucocephala leaves. The samples from both extraction methods were
then clarified by boiling it with 30 mg activated carbon and filtered. The absorbance
of diluted aliquot of 1-2-3-4-5 mL was then obtained from spectrophotometer at the
wavelength of 535 nm. The concentration of mimosine from soxhlet extraction with
distilled water was found to be higher compared to the digestion method but in
contrast with ethyl acetate solvent extraction.

Keywords: Mimosine; Digestion; Soxhlet; Leucaena leucocephala.

25
 027
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of Accelerated Stability Test on

Characteristics of Emulsion Systems with
Chitosan as a Stabilizer

Witta Kartika Restua, Yulianti Sampora, Yenny Meliana, Agus

Haryono*
a
Research Center for Chemistry, Indonesian Institute of Sciences, Puspiptek Serpong, 15314,
Indonesia

Abstract
Chitosan has been used as a stabilizer in the preparation of emulsion systems with
Squalene as an oil phase, then Tween 80 and Span 85 as a surfactant. Water
phase, oil phase and chitosan as a stabilizer stirred with a homogenizer speed of
15,000 rpm for 30 minutes. The results of accelerated stability test with extreme
temperatures of 4ᵒC and 40ᵒC showed significant changes in viscosity, density
and degree of stability. However, the degree of acidity was relatively still same in
which closer to normal pH (6.3-6.4). The particle size analysis showed the size
around 3 micrometers. This emulsion system can be applied as the media of active
compound delivery, for example is the emulsion system of topical and oral
preparation to overcome celullite problem.

Keywords : chitosan, emulsion, stabilizer

26
 028
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Formulation of Instant Functional Drinks Based

on Ginger and DFA III

D. Ratnaningrum*1, Thelma A. Budiwati1, W. Kosasih1, and S.

Pudjiraharti1
1
Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Sangkuriang Bandung
40135 – Indonesia.

Abstract
Difructose anhydride III (DFA III) is a functional disaccharide which is reported to
stimulate absorption of calcium in the intestines and increase bone calcium and
density. Thus, DFA III was considered for prevention of osteoporosis. The aim of
this study is to formulate functional drink with DFA III as active ingredient for bone
health, and instant ginger as well as to evaluate the formula based on the
organoleptic test and physicochemical properties of the selected formula. The
experiment was started with instant ginger preparation followed by formulation. Six
formulas consist of a constant concentration of DFA III and different concentrations
of instant ginger, skim milk, vegetable creamer, and calcium lactate were
developed. To find out acceptability of the formulas, hedonic test was performed by
involving 30 panellists. The results showed that F6 which contains of 25 g instant
ginger, 9.375 g of skim milk, 6.25 g vegetable creamer, 0,385 g calcium lactate, and
5 g of DFA III was indicated as the most favourable formula chosen by the
panellists with the same highest preference values (44.4 %) for colour, aroma, and
taste compared to other formulas. Physicochemical analysis results also
demonstrated that F6 has suitable characteristics mainly in terms of solubility,
hygroscopicity, as well as water content.

Keywords: Functional drinks, osteoporosis, DFA III, instant ginger, hedonic test.

27
 029
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Fabrication of SiO2-TiO2/PVDF Copolymer

Nanofiber Composite by Electrospinning Process

Muhamad Nasira , Juliandrib, Bambang Prihandokoc

a
Research Center for Chemistry, Indonesian Institute of Science (LIPI), Jalan Cisitu
Sangkuriang, Bandung 40135,Indonesia
a
Department of Chemistry,Padjadjaran University,Jatinangor,Sumedang 40135, Indonesia
b
Research Center for Phisyc, Indonesian Institute of Science (LIPI),Puspitek ,Serpong 40135,
Indonesia

Abstract
Multifunctional nanocomposite material is a key material component for the future. It
is indicated by many studies have been conducted by researchers in university,
research institute and industries. Silicon dioxide (SiO2), titanium dioxide (TiO2) and
polyvinilidene fluoride copolymer are very important material due to their excellent
properties. In this research paper, we have successfully synthesized SiO2-
TiO2/PVDF copolymer nanofiber composite by electrospinning process. SiO2-
TiO2/PVDF copolymer nanofiber composite has smooth surface morphology without
bead on nanofiber string after confirming by scanning electron microscopy (SEM).
Nanofiber composite has average diameter of 350 nm. FTIR and XRD structure
analysis of nanofiber composite show us that PVDF copolymer in nanocomposite
has a mixed α and β phase crystal structure. Crystal phase of TiO2 in nanofiber
composite was in rutile form.

Keywords: SiO2-TiO2 nanoparticle, electrospun SiO2-TiO2/PVDF copolymer

nanofiber composte, and electrospinning,

28
 030
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Preparation of Inulin from Dahlia Tubers

Wawan Kosasiha, Sri Pudjirahartia, Diah Ratnaningruma, Rina

Andryania, Sri Priatnia
a
Research Center for Chemistry Indonesian Institute of Sciences, Jl Sangkuriang Bandung,
Indonesia 40135

Abstract
Inulin is polysaccharide chains comprised primarily of linked fructose molecules that
are bifidogenic. Inulin-type prebioticsare used as functional food ingredients in
beverages, yogurts, biscuits, and spreads; they are also used as dietary
supplements.The objective of this study was to increase the yield of inulin by
improving the inulin extraction from dahlia tubers. Inulin wasisolated from fresh and
chips of dahlia tubers. The dahlia tubers was blanched, sliced and blended by
adding the hot water in ratio1:1. inulin extract was evaporated and precipitated by
95% ethanol for 24 hours in a room temperature. Inulin was separated anddried at
50°C for 24 hours. Results showed that the best ratio of inulin extract and ethanol is
1 : 2 and the highest yield of inulinpowder was produced from inulin extract of 30.0
% brix. Moreover, the yield of inulin which refer to fresh dahlia tubers isbetween 6.0
- 9.5 % w/w.

Keywords: inulin, precipitation, dahlia, tubers

29
 031
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of alkaline pretreatment on properties of

lignocellulosic oil palm waste

Vera Barlianti*, Deliana Dahnum, Hendris Hendarsyah, and

Haznan Abimanyu
Research Centre for Chemistry, Indonesian Institute of Sciences (LIPI), Kawasan Puspiptek
Serpong, Tangerang Selatan 15314, Indonesia

Abstract
As a biggest palm oil producer in the world, Indonesia also produces a huge
amount of oil palm waste, like oil palm empty fruit bunch (EFB) and oil palm fronds
(OPF). Oil palm EFB and OPF contain cellulose that can be converted to bioethanol
through pretreatment, saccharification, and fermentation processes. The
pretreatment is required to liberate the cellulose from lignocellulosic matrix. One of
pretreatment method is chemical pretreatment process using alkaline solution at
high pressure and temperature. This paper reports the influence of alkaline
pretreatment to chemical composition, cellulose crystallinity index, and morphology
of oil palm EFB and OPF. The results show that increasing of alkaline concentration
causes decreasing of lignin content from 25.83% to 13.61% for oil palm EFB and
from 30.92% to 19.23% for OPF at 10% of NaOH solution. The lowest cellulose
crystallinity index of oil palm EFB and OPF are achieved at 10% and 8% of NaOH
solution, respectively. The result also indicates the changing of oil palm EFB and
OPF morphology. After alkaline pretreatment, the surface of oil palm EFB and OPF
look rougher than the raw material, some holes are formed on them.

Keywords: Alkaline pretreatment; cellulose crystallinity; morphology; lignin; oil

palm empty fruit bunches; oil palm fronds

30
 033
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Analytical Method Development for Bisphenol A

in Tuna by using
High Performance Liquid Chromatography-UV

Yosi Aristiawan*, Nurhani Aryana, Dillani Putri, Dyah Styarini

Metrology in Chemistry Laboratory, Research Center for Chemistry-Indonesian Institute of
Sciences Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Indonesia 15314

Abstract
Bisphenol A (BPA) detection in food has gained research interest in recent years.
This attention is based on BPA characteristic, easy to migrate and potentially
hazard for human, and their usage in many packaging products polluting food and
environment. In this research, a reverse phase high liquid chromatography coupled
with UV detector (HPLC-UV) was developed for determination BPA in tuna. The
analytical method was validated based on the following criteria: linearity, precision,
repeatability, limit detection, and recovery. Excellent linear correlation with R2 =
0.9992 was observed over the range of 2 to 250 mg/kg. The detection limit was
calculated to be 0.8 mg/kg, while the limit of quantification (LOQ) value of the
validated method was measured to be 1.5 mg/kg. Intra-day and intermediate
precision (RSD, %) were 3.42 and 3.50, respectively. Good recoveries of 89.84%
with RSD% = 5.41 were obtained at level concentration 32 mg/kg. The proposed
method was found to be suitable and precised for the determination of BPA in tuna
by using HPLC-UV.

Keywords: Bisphenol A; HPLC-UV; method validation; food

31
 033
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Laboratory Performance Evaluation on Analysis

of Cadmium and Lead in Herb Through
Participation in The Regional Laboratory
ComparisonAPMP QM-P10

Yohanes Susanto Ridwanaand Willy Cahya Nugrahab

ab
Research Center For Chemistry-Indonesian Institute of Sciences
Jl. Cisitu-Sangkuriang, Bandung 40135

Abstract
The ubiquitous presence of heavy metals in the environment arising from various
natural and anthropogenic activities have led to the accumulation intrusion of these
substances into agricultural products including herbal plants. With the rapid growth
of herbal medicine trade market, extensive surveillance monitoring and laboratory
testing of heavy metals in herbal samples are carried out worldwide nowadays.
Those surveillance activities are very much rely on the quality of the data produced
by analytical laboratory. As analysis of heavy metals have been one of several main
interests of our laboratory of Research Center for Chemistry, then participation on
the international laboratory comparison such as APMP.QM-P10: Cadmium and
Lead in Herb is a valuable effort for evaluate laboratory performance in this area of
analysis. Several trial run and validation had been performed on the proficiency
samples, including sample preparation, level of method blanks, use of matrix
modifiers, precision and also trueness check using suitable reference material. Z-
scores of 0.787 and 1.105 for cadmium and lead respectively have obtained as
result of participation. It was found that the z-scores were satisfactory compared to
the satisfactory limit of -2 ≥ z-score ≤ 2. These results provide strong confirmation of
maintained capability of Research Center for Chemistry on heavy metals analysis,
particulary in herb.

Keywords: cadmium, lead, herb, APMP intercomparison

32
 034
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Surface Analysis of Carboxylic Acids Modified

SiO2 on PMMA/ENR 50/LiBF4 Electrolytes

Siti Izzati Husna Mohd Azuana,*, Famiza Abdul Latifa, Sharil

Fadli Mohamad Zamria
a
Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor,
Malaysia

Abstract
Incompatibility between hydrophilic filler and the hydrophobic polymer matrix has
limited the performance of polymer electrolytes. Inclusion of silicon dioxide (SiO2)
filler on polymethyl methacrylate/50 % epoxidised natural rubber (PMMA/ENR 50)
was proven increased the ionic conductivity of doped lithium tetrafluoroborate
(LiBF4). However, SiO2 particle is agglomerated due to the presence of silanol
groups (Si-OH) on its surface and then led towards the development of hydrogen
bonding. Therefore, the surface of the SiO2 has been modified with carboxylic acids
(COOH) that would give hydrophobic nature. The result shows carboxylic acids
modified SiO2 (COOH-SiO2) filler was successfully minimized the amount of Si-OH
on the SiO2 surface. Compatibility between COOH-SiO2 and blend systems was
improved as COOH-SiO2 filler well dispersed on PMMA/ENR 50/LiBF4 electrolytes.
SiO2 filler was modified with butanoic acid (C4-SiO2), octanoic acid (C8-SiO2),
dodecanoic acid (C12-SiO2) and hexadecanoic acid (C16-SiO2). Fourier transform-
infrared (FT-IR) was used to investigate the functional groups of interested peaks
on the COOH-SiO2 surface. The composition of carbon elements on COOH-SiO2
filler was studied using CHNS analysis. The effects of COOH-SiO2 on the
dispersion in the blend systems were evaluated by scanning electron microscopy
(SEM) with EDX analysis. Blend properties of PMMA/ENR 50/LiBF4 electrolyte
systems were improved upon the addition of modified SiO2 filler.

Keywords: Silicon dioxide, modified filler, modifier agents

33
 035
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Electrochemical Lipase Biosensor Based On

1-(2-Ethoxy-Ethyl)-1-Methylpyrrolidinium
Bis(Trifluoromethylsulfonyl)imide For
Triglyceride Determination In Olive Oil

Nurul Huwaida Roslia, Zainiharyati Mohd Zaina*, Nor Monica

Ahmadb
a
Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor,
Malaysia
b
Faculty of Applied Sciences, Universiti Teknologi MARA, 47000 Kuala Pilah, Negeri
Sembilan, Malaysia

Abstract
Electrochemical determination of Triglyceride (TG) based on Lipase enzyme was
used here to hydrolyze TG into Glycerol andFatty acid. We incorporated ionic liquid
(IL) that has a high ionic conductivity and well biocompatibility to enhance
theelectrochemical response. In this study, 1-(2-ethoxy-ethyl)-1-methylpyrrolidinium
bis(trifluoromethylsulfonyl)imide,[C2OC2C1pyrr][N(Tf)2] was used. Hence, the
mixture of [C2OC2C1pyrr][N(Tf)2]-GA-Lipase has been immobilized on the surface
of screen printed carbon electrode (SPCE) by drop cast technique for the
determination of Triglyceride concentrations in oliveoil. The electrochemical
performance of C/[C2OC2C1pyrr][N(Tf)2]-GA-Lipase electrode has been
investigated using cyclicvoltammetry (CV). The surface morphology of the modified
SPCE was studied. The optimization parameters ofC/[C2OC2C1pyrr][N(Tf)2]-GA-
Lipase electrode were carried out, and shows that pH 7, 30°C and 5 % of Lipase
enzyme loadingresulted in optimum performance. The biosensor was applied to
detect Triglyceride with limit of detection (LOD) and responsetime of 0.92 mM and
37 seconds respectively. A linear calibration range from up to 2.68 mM was
obtained. Common interferingcompounds on the sensor were investigated as well.

Keywords: Triglyceride (TG); Lipase; Ionic Liquid; Cyclic voltammetry (CV); Screen
Printed Carbon Electrode (SPCE).

34
 036
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Occurrence and Removal of Pharmaceutical

Products in Municipal Wastewater

Siti Norbayu Mohd.Subari, Rozita Osman , Halila Jasmani,

Norashikin Saim*
Faculty of Applied Science, Universiti Teknologi MARA, 40450 Shah Alam, Selangor,
Malaysia

Abstract
The occurrence of six pharmaceuticals in a conventional wastewater treatment
plant was investigated. Selected pharmaceuticals included a stimulant (caffeine),
two analgesics (acetaminophen and salicylic acid), three non-steroidal anti-
inflammatory drugs (mefenamic acid, naproxen and diclofenac). Water samples
from the influent and effluent were collected periodically within 6 months.
Concentrations of pharmaceuticals in the influent and effluent were determined
using online solid phase extractionliquid chromatography with diode array detector.
Among the pharmaceuticals studied, caffeine, acetaminophen, salicylic acid and
diclofenac were detected in influent at the concentrations ranged from 0.81 to 48.2
µg L-1. Diclofenac persisted in effluent with average removal efficiency of 37%
while caffeine, acetaminophen and salicylic acid showed average removal efficiency
between 93 and 100 %. The inefficiency of water treatment plant studied in
removing diclofenac can significantly increase its occurrences in the receiving
water.

Keywords: pharmaceuticals; occurrence; wastewater; Malaysia

35
 037
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Pyrolysis-gas chromatography-mass
spectrometry (Py-GC-MS)
analysis of automotive paints traces

Raja Zubaidah Raja Sabaradin, Rozita Osman, Halila

Jasmani, Norashikin Saim*
Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor,
Malaysia

Abstract
Pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) was applied in the
analysis of red car paint samples of variouscar models. Comparison of pyrograms
based on qualitative approach was able to differentiate paint samples. Py-GC-MS
was ableto identify the pyrolysis products, allowing discrimination of samples based
on their chemical composition. This studydemonstrated the ability of Py-GC-MS in
increasing the evidence value of car paints in forensic investigation.

Keywords: car paint; pyrolysis-gas chromatography

36
 038
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Characteristics of Feed Coal and Particulate

Matter in the Vicinity
of Coal-fired Power Plant in Cilacap, Central Java,
Indonesia

Diah Dwiana Lestiania*, Muhayatun Santosoa, Syukria

Kurniawatia, Natalia Adventinia, Djoko Prakoso D.Aa
a
National Nuclear Energy Agency-BATAN, Center for Applied Nuclear Science and
Technology, Tamansari 71, Bandung 40132, Indonesia

Abstract
The Indonesian government has launched the development of electrical power plant
10,000 MW program to supply the electricityin Indonesia which is focused on coal
power plant. Minor and trace element characterization of coal is extremely important
toassess their potential for pollution, as well as characterization of the fine
particulates matter (PM2.5) emitted from a coal-fired powerplant which could be
harmful to human health and environmental. In this study, characterization of feed
coal and fine particulatematter in the vicinity of a coal-fired plant as an assessment
of environmental impact on coal combustion was carried out. Theparticulate matters
PM2.5 were collected in two sites in the vicinity of 2 x 300 MW coal-fired power
plant in Cilacap, Central Java.The feed coal was characterized using instrumental
neutron activation analysis-INAA. Method validation was done by analysis
ofstandard reference material NIST SRM 1633b coal fly ash. The chemical
composition of PM2.5 have been measured using energydispersive X-ray
Fluorescence-EDXRF. The validation of XRF measurement was applied using
standard reference material NISTSRM 2783 Air Particulate on Filter Media. The
results showed that several minor and trace elements in coal were still in the range
of other world coals. The elemental concentration of PM2.5 especially for the toxic
elements as well as the mass and black carbonin the vicinity of the coal fired power
plant was low or within the range of published data for air ambient in urban and
rural areas.

Keywords: particulate matter; trace elements; coal fired power plant; Cilacap;
Central Java; Indonesia

37
 039
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Extraction of Citalopram and Main Metabolites In

Rabbit Urine Samples from Fatal Citalopram
Intoxication Using QuECheRS and High
Performance Liquid Chromatography

Siti Aisyah Shamsuddina, Rumiza Abd Rashidb, Farida Zuraina

Mohd Yusofa,b,*
a
Biology Department, Faculty of Applied Sciences, Universiti Teknologi MARA,
40450 Shah Alam, Selangor, Malaysia
b
Institute of Forensic Science, Universiti Teknologi MARA, Jalan Klang Lama,
Kuala Lumpur, Malaysia

Abstract
Citalopram is a selective serotonin reuptake inhibitor (SSRI) drug which prescribed
for the treatment of psychiatric disorder . Citalopram is metabolized to desmethyl
citalopram and didesmethyl citalopram after oral administration. In this study, we
described the modification of extraction technique of QuEChERS (Quick, Easy,
Cheap, Effective, Rugged, Safe) to quantify the citalopram and its metabolites
concentration in urine samples by high performance liquid chromatography coupled
with diode array detector. Analysis was performed using C8 column under isocratic
elution of 0.8 mL/min running through the mobile phase consisting of 55% (v/v) 20
mM ammonium acetate buffer (pH 7) and 45% (v/v) acetonitrile at 40°C. The
detection of analytes was set at a wavelength of 240 nm. Separation of analytes
was done in less than 6 minutes after injected with 10 µL of aliquot. The analytes
were greatly extracted from the urine sample after clean-up with 25 mg of PSA
sorbent and magnesium sulphate. This method was optimized and validated to
obtain highest linearity of regression coefficient (R2) which is 0.999 estimated from
the calibration curve range from 1000 ng/mL to 100 ng/mL, with the high precision
(RSD <4%) and accuracy (75% – 110%). The method was revealed high sensitivity
with LOD and LOQ range between 24.10 ng/mL to 343.50 ng/mL and 80.34 ng/mL
to 1.14µg/mL, respectively. From the analysis, citalopram and its main metabolites
were detected and quantified in range between 0.3965 µg/mL to 16.5203 µg/mL.
Thus, this could provide a reliable technique on detection and quantitation of the
drug from biological samples which could be applied in forensic investigations.

Keywords: citalopram; desmethyl citalopram; didesmethyl citalopram; QuEChERS;

high performance liquid chromatography; PSA sorbent

38
 040
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis of Vanillin Derivatives Using

CuCl(OH).TMEDACatalyst

Michelle Ongkosoewitoa, Hery Sutantoa, Yulia Anitab*

a
Swiss German University, BSD City, Tangerang, 15339, Indonesia
b
Pusat Penelitian Kimia LIPI, Serpong, Banten, 15314, Indonesia

Abstract
Vanillin has phenolic group in its structure which enabled this molecule to become
antioxidant. This research investigatedsynthesis reaction of vanillin derivatives
using a metal catalyst, namely CuCl(OH).TMEDA. This synthesis reaction
usedoxidative coupling mechanism under aerobic condition at room temperature.
The synthesis product was analyzed using thin layerchromatography and further
purified with column chromatography. One isolate from column chromatography
was analyzedusing LC/MS/MS. LC/MS/MS spectrum supported the facts that the
synthesis product was a dimer of vanillin. The isolateproduct of synthesis reaction
was analyzed for its antioxidant activity using DPPH radical scavenging assay.
According toprevious research, dimer of phenol has higher antioxidant activity than
its monomer, however dimer of vanillin synthesized usingCuCl(OH).TMEDA has
lower antioxidant activity. The decrease in antioxidant activity was suspected from
the structure whichpresent in keto form.

Keywords: Vanillin; CuCl(OH).TMEDA; Catalyst; Oxidative Coupling; Dimerization;

Antioxidant

39
 041
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Application of mint oil as an antimicrobial agent

in the development of liquid surface disinfectant
for food contact surfaces

Evita Santoso, Hery Sutanto*, Elisabeth K. Prabawati

Faculty of Life Sciences and Technology, Swiss German University, EduTown BSD City,
Tangerang 15339, Indonesia

Abstract
The antibacterial activity of mint essential oil as a natural ingredient offers a
potential replacement for chemical disinfectant for food contact surfaces in food
industries. The purpose of this research was to develop a mint oil-based
disinfectant and test for its efficacy. Prior to the main research, the minimum
inhibitory concentrations of essential oil of two species of mint, peppermint (Mentha
piperita) and cornmint (Mentha arvensis), against Staphylococcus aureus and
Escherichia coli were assessed. The type of mint oil that has higher activity against
both types of bacteria mentioned previously was used in the disinfectant formula.
The disinfectant was made of 1.25% peppermint oil, Tween 80 as a stabilizer, and
water. Three formulas of disinfectant were made by varying the concentration of
Tween 80, which were 0.5%, 5%, and 10%, to analyze the stability of the emulsion
formed. The result of the disinfectant efficacy testing against Staphylococcus
aureus and Escherichia coli shows that there were no significant differences (p ≤
0.05)between the number of bacteria before and after contact with the disinfectant
for both types of bacteria. Furthermore,the increase of Tween 80 concentrations
decreases the reduction of the number of bacteria after contact with disinfectant.
This suggests that Tween 80 reduces the antimicrobial activity of peppermint oil.

Keywords: Mentha piperita; Mentha arvensis; Essential oil; Antimicrobial activity

40
 042
International Symposium on Applied Chemistry 2015
(ISAC 2015)

A Physico-Chemical Analysis of Coconut Shell

Powder

Chinthani D Liyanagea*, Mevan Pierisb

a
Department of chemistry, University of Colombo, Colombo 3, Sri Lanka
b
Institute of Chemistry Ceylon, 341/22 Kotte road, Welikada, Rajagiriya, Sri Lanka

Abstract
Coconut is a major plantation crop of Sri Lanka and coconut millers are striving to
add value to coconut shells being generated as an agricultural waste material. A
leading coconut miller is mechanically grinding the shells into powder to be used by
industry as a filler material or in any other way that would add value. In this study
the coconut shell powder (CSP) has been analysed both physically and chemically
to have a better understanding of the material its properties. Laser diffraction
showed a broad distribution of sizes with a mean particle size of 34.2 microns, and
by using scanning electron microscopy it was possible to recognize a variety of
sizes, shapes and structures. The thermo-chemical character of CSP was studied
over a temperature range of 30 °C – 800 °C using a thermogravimetric analyser.
CSP was seen to pyrolyse best between 220 °C - 380 °C with a residue of 16.2%
left at the end of the pyrolysis. In order to understand the thermo-chemical behavior
of CSP more fully and to estimate the chemical composition, the lignin content was
determined by the ASTM D 1106 – 96 method and a thermogravimetric analysis
(TGA) and a differential thermogravimetric analysis (DTG) were done on the lignin
as well. The CSP was pyrolysed at 550 °C to determine the ash content and using
atomic absorption spectroscopy the types of metal in ash were determined. Apart
from silica which could not be measured, sodium, potassium, zinc, calcium and iron
were found to be the main metals. Fourier transform infrared spectroscopy (FTIR)
was used for a better understanding of the chemical structure of CSP.

Keywords: Coconut shell powder; lignin; laser diffraction; thermogravimetric

analysis; scanning electron microscopy; atomic absorption
spectroscopy; FTIR.

41
 043
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Development of Validated Method for

Determining the Organochlorine Pesticide
Risidues in Lettuce Using QuEChERS Technique

Evita Boesa*, R. Tina Rosmalinaa Yohanes Susanto Ridwana,

Willy Cahya Nugrahaa, and Retno Yusiasiha
a
Research Center for Chemistry- Indonesian Institute of Sciences,
Jalan Cisitu Sangkuriang Bandung 40135, Indonesia

Abstract
A simple method was evaluated for the determination of 18 pesticide residues
(alpha-BHC, betha-BHC, gamma-BHC, delta-BHC, Heptachlor, Aldrin, α-
Endosulfan, DDE, Dieldrin, Endrin , β-Endosulfan, DDD, Endrin Aldehid, Endosulfan
Sulfat, DDT, Endrin Keton, and Metoxychlor in lettuce where pesticide residues
were extracted and cleaned using a buffered QuEChERS (Quick, Easy, Cheap,
Effective, Rugged and Safe) method, followed by GC-MS analysis. This extraction
method involves first mixing the sample with acetonitrile and permit the salt out
liquid-liquid partitioning step using anhydrous MgSO4 and sodium acetate. After
shaking and centrifugation, cleanup was done by dispersive solid phase extraction
(d-SPE) using 150 mg of anhydrous MgSO4, 150 mg of PSA, and 50 mg of C-18
per milliiter of extract. The optimized analytical conditions were evaluated in terms
of recoveries, repeatability, limits of quantitation and matrix effects for lettuce
sample. Use of matrix matched standards provided acceptable results for most
pesticides with overall average recoveries between 70 and 120 % and consistent
RSD < 15 % for organochlorine pesticides.

Keywords: Organochlorine,pesticide, QuECheRS, GCMS

42
 044
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Application of mint oil as an antimicrobial agent

in homemade mayonnaise

Isabella Kartika Saria, Hery Sutantob, Elisabeth K. Prabawatia*

a
Swiss German University, Department of Food Technology, EduTown BSD City,
Tangerang, Indonesia
b
Swiss German University, Department of Chemical Engineering, EduTown BSD City,
Tangerang, Indonesia

Abstract
The essential oil extract of Mentha arvensis and Mentha piperita from Lembang,
West Java, Indonesia were analyzed for their antimicrobial activity in direct
application and homemade mayonnaise. The antimicrobial activity was assessed
using disk diffusion analysis for qualitative observation of the activity of the extract
against Salmonella sp. The result was showing the activity of Mentha sp. essential
oil extract to inhibit the growth of Salmonella sp., where the clear zone diameter
was increase proportionally with the oil concentration. The quantitative antimicrobial
analysis was performed using macro broth dilution assay, which was followed by
determination of minimum bactericidal concentration. Essential oil extract of Mentha
arvensis was showing better antimicrobial activity in killing Salmonella sp., where
the minimum concentration required to kill the pathogen was 0.2929% and the
minimum concentration required by essential oil extract of Mentha piperita was
0.3906%. Cornmint oil, the oil extract of Mentha arvensis, was applied in
mayonnaise as it possessed the best antimicrobial activity. TPC was conducted for
14 days at 4°C storage temperature. The TPC results of total bacteria and
Salmonella sp. were showing no preservative ability of cornmint oil in homemade
mayonnaise.

Keywords: Essential oils, Mentha sp., mayonnaise, antimicrobial, Salmonella sp.

43
 045
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Ultrasonic approach in butterfly pea extraction

and extract sterilization by ultrafiltration for eye
drop active ingredient

Bea Anthika*, Samuel P. Kusumocahyo, Hery Sutanto

Faculty of Life Sciences and Technology, Swiss German University, South Tangerang 15339,
Indonesia

Abstract
The aim of this study is to evaluate butterfly pea ultrasound-assisted extraction
(UAE) and sterilize the extract using ultrafiltration (UF) membrane for use as an
active ingredient in phytopharmaceutical eye drops. The effect of petal-to-leaf (PTL)
ratio and extraction time on anthocyanin concentration and antibacterial activity has
been studied. The result showed that the optimum configuration was PTL ratio of
1:0 and extraction time of 30 minutes, which significantly yielded anthocyanin
concentration of 35.41 ± 0.62 mg/l and exhibited the highest antibacterial activity in
terms of inhibition zone against S.aureus of 14.75 ± 1.06 mm. Subsequently,
feasibility of using UF membrane to sterilize the butterfly pea extract was also
studied. Number of bacteria in the extract was varied to check whether UF was able
to sterilize the extract regardless of the degree of bacterial contamination. The
results showed no presence of bacteria in the permeate during 21 days of
observation and an increase in anthocyanin concentration as well as antibacterial
activity of the extract. In conclusion, UF was very effective to sterilize butterfly pea
extract without the application of heat, thus, preserve the heat-sensitive compounds
such as anthocyanins.

Keywords: butterfly pea; ultrasound-assisted extraction (UAE); sterilization;

ultrafiltration (UF) membrane; anthocyanins.

44
 046
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Iodination Method of Quercetin for Synthesis of

Anticancer Labelled Compound

Maula Eka Sriyania, Dian Ayu Utamib, Eva Maria Widyasaria,

Muharam Marzukib
a
National Nuclear Enenrgy Agency, Jl. Tamansari No. 71 Bandung 40132, Indonesia
b
UNJANI, Cimahi Indonesia

Abstract
Quercetin is a bioflavonoid that has high potential as a candidate of anticancer. The
in-vivo study of radicals detection methods in the body that causing cancer is
limited. Therefore, the radiolabeled of an antioxidant compound to detect the
radicals noninvasively are very useful. This research is conducted as a preliminary
study for synthesis of anticancer therapeutic agent labelling compound using
iodinated quercetin. The iodination of quercetin was conducted with oxidation of
iodine using chloramine-T method. The result showed that the reaction of equimolar
KI, Quercetin and chloramine-T, gave the product of sodium,-5’,6,8-triiodoquercetin.
This method could be suitable to be used in the labelling of quercetin, using beta
and gamma emitter radioactive of iodine-131 for anticancer therapeutic agent.

Keywords: quercetin; iodinated; anticancer; antioxidant

45
 047
International Symposium on Applied Chemistry 2015
(ISAC 2015)

46
 048
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Influence of Carbon Black and Silica Filler on the

Rheological and Mechanical Properties of Natural
Rubber Compound

Ika Maria Ulfah*, Riastuti Fidyaningsih, Sri Rahayu, Diah Ayu

Fitriani, Dita Adi Saputra, Dody Andi Winarto, and Lies A.
Wisojodharmo
Center for Materials Technology, The Agency for the Assessment and Application of
Technology, 224th Building, Puspiptek Area, Tangerang Selatan, 15314, Indonesia

Abstract
Rubber compounds are reinforced with fillers such as carbon black and silica. In
general, filled rubber compounds shows smooth rheological behavior and
mechanical properties. Variation in rheological behavior and mechanical properties
was studied in terms of the filler composition using natural rubber compounds filled
with both carbon black and silica CB/Si = 0/60, 20/40, 30/30, 40/20 and 60/0 phr
(parts per hundred rubber is parts of any non-rubbery material per hundred parts of
raw gum elastomer (rubbery material)). The rheological behaviour can be showed in
measurement of Mooney viscosity and cure time. The Mooney viscosity of rubber
compounds increase with the increasing the carbon black in the compounds. The
compound filled with CB/Si of 30/30 and 60/0 showed abnormal rheological
behaviour in which the cure time decreased suddenly and the increased at certain
ratio during the measurement. The mechanical properties such as hardness,
abrasion resistance and tensile stress at 300% elongation were studied. In the
hardness and abrasion resistance measurement, the higher ratio CB/Si decrease
contribution of silica, which resulting smaller of hardness value. Ratio CB/Si 40/20
gives an optimum filler blended. It is also clearly understood that higher abrasion
resistance mainly due to the lower hardness value under the same condition. The
tensile stress at 300% elongation of rubber compound increased with the increasing
carbon black filler.

Keywords: filler-filler interaction; carbon black; silica; rheological behaviour;

mechanical properties

47
 049
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Biotransformation of eugenol to dehydroeugenol

catalyzed by Brassica juncea Peroxidase and its
cytotoxicity activities

Yulia Anitaa, Andini Sundowoa, Puspa Dewi N. La, Euis

Filaillaa, Hani Mulyania , Chandra Risdiana, Sofna
Banjarnahora, Muhammad Hanafia and Enade P istyastonob
a
Research Centre of Chemistry Indonesian Institute of Science, Kawasan Puspiptek
Serpong, 15314, Indonesia
b
Pharmaceutical Technology Laboratory, Faculty of Pharmacy,Sanata Dharma University,
Yogyakarta, Indonesia

Abstract
A crude peroxidase biocatalyst extracted from sawi hijau (Brassica juncea) was
used to produce dimerization phenolic synthesized from eugenol. The result from
MS and NMR analysis showed that the reaction between eugenol substrate and
H2O2 with crude extract enzyme produced a new chemical compound. The
chemical structure of new compound showed coupled result in ortho-ortho position
with C-C coupled derivative, dehydrodieugenol. It was obtained as brownish white
crystal needles with yield 10 %. This compound displayed high cytotoxicity effect on
T47D breast cancer cell is compared to the eugenol monomer that showed less
cytotoxic activity.

Keywords: Peroxidase; Eugenol; Dehydioeugenol; Dimerization; Oxidative

coupling

48
 050
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Screening and Characterization of Levan

Secreted by Halophilic Bacterium
of Halomonas and Chromohalobacter Genuses
Originatedfrom Bledug Kuwu Mud Crater

Daris Qodarisman Nasira, Deana Wahyuningrumb, Rukman

Hertadia*
a
Biochemistry Research Divisions, Bandung Institute of Technology,
Jalan Ganeca no. 10 Bandung 40132, Indonesia.
b
Organic Research Divisions, Bandung Institute of Technology,
Jalan Ganeca no. 10 Bandung 40132, Indonesia.

Abstract
Five bacterial isolates from the genus of Halomonas and Chromohalobacter
isolated from Bledug Kuwu mud crater, located inmainland Purwodadi-Grobogan,
Central Java, Indonesia, have been assayed for levan production. Initial screening
was conductedon modified Belgith medium using sucrose as the major carbon
source, in which the colonies of levan-producing bactera willhave a slimy mucoid
appearance when grown on this medium. The screening results showed only one
positive bacterial species,which was Chromohalobacter japonicus BK AB18 that
identified as potential levan producer. Thermal stability of the isolatedlevan has
been charachterized by TGA, which gave approximate decomposition temperature
o
about 211 C. The structure of thelevan has been elucidated by FTIR and NMR
spectroscopies. FTIR spectrum of the isolated levan displayed high similarity tothat
of levan isolated from Bacillus methylotrophicus. The chemical shifts of carbon and
proton NMR spectra of the isolatedlevan also exhibited high similiarity to those of
levan isolated from Pseudomonas fluorescens and Zymonas mobils.

Keywords: levan, Halomonas and Chromohalobacter japonicus BK-AB18

49
 051
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Isolation of Cellulose Nanocrystals from Bacterial

Cellulose Produced from Pineapple Peel Waste
Juice as Culture Medium

Budiman Anwara,b,*, Bunbun Bundjalib, I Made Arcanab*

a
Department of Chemistry Education, Indonesia University of Education, Jl. Dr. Setiabudi 229,
Bandung 40154-Indonesia
b
Inorganic and Physical Chemistry Research Division, Bandung Institute of Technology, Jl.
Ganesha 10, Bandung 40132-Indonesia

Abstract
Cellulose nanocrystals (CNC) were isolated by the sulfuric acid hydrolysis of
bacterial cellulose (BC) produced by Gluconacetobacter xylinus using pineapple
peel waste juice as culture medium. Optimal conditions of the acid hydrolysis were
obtained at concentration of sulfuric acid of 50%, hydrolysis time ranging 25~40
O
minutes and temperature at 50 C. The FTIR study shows that the CNC spectrum
were similar with BC which indicated that both have similar chemical composition;
-1 -1
however there are some peaks alteration at 2900 cm and 1430 cm which
indicated that BC has changed into CNC. The crystallinity of CNC determined by
FTIR was found to be higher than BC. By TEM observation, the CNC particles had
average length of 325 nm and diameter of 25 nm with average aspect ratio (L/D)
around 13. The TEM images also show the morphology of CNC that has rod-like
structure.

Keywords: Cellulose Nanocrystals; Bacterial Cellulose; Pineapple Peel Waste,

FTIR

50
 052
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Elucidation of Active Site Protective Residues in

Rhizomucor miehei Lipase by Targeted Molecular
Dynamics Approach

Yusran Abdillah Muthahari, Rukman Hertadi*

Biochemistry Division, Chemistry Department, Institut Teknologi Bandung, Jl. Ganesha No.
10, Bandung 40132, Indonesia

Abstract
Rhizomuor miehei Lipase (RmL) is a type of lipase that has a single lid segment
playing important role in its catalytic activity. RmL lid segment is a long helical chain
located at residues 85 to 92 acting as a gate to mediate substrate binding and
product releasing by making open and close dynamics movement. In the present
study, we were interested to evaluate the molecular mechanism of opening RmL lid
segment from its closed state in aqueous solvent by using targeted molecular
dynamics (TMD) approach. The external forces about 0.125 kcal/(mol.Å) was
applied in TMD to push the closed state of RmL lid toward the targeted open state.
The results showed that RmL lid was fail to reach the targeted structure, in which
the final structure was stacked at about 65% opening. The comparative structural
analysis between the partially and fully open states revealed the caused of the
sterical hindrance preventing the lid segment to reach the fully open state. Strong
hydrophobic interactions among Trp88 with the surrounding non-polar residues in
the active site was the major caused for the sterical hindrance. This restriction will
protect the active site of the enzyme to be fully exposed to the solvent, thereby
minimizing the accessibility of non-substrate molecules into the active site.

Keywords: Rhizomucor miehei Lipase, Lid, Active Site Protecting Residues, Targeted
Molecular Dynamics.

51
 053
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Isolation and characterization of levan from

moderate halophilic bacteria Bacillus
licheniformis BK AG21

Mamaya, Deana Wahyuningrumb, Rukman Hertadia*

a
Biochemistry Research Division, Bandung Institute of Technology, Jl. Ganeca 10 Bandung
40132, Indonesia.
b
Organic Chemistry Research Division, Bandung Institute of Technology, Jl. Ganeca 10
Bandung 40132, Indonesia.

Abstract
Levan is fructose polymer as the result of biosynthesis by levansucrase. This study
is aimed to explore the potential of moderate halophilic bacteria Bacillus
licheniformis BK AG21 isolated from Bledug Kuwu, Purwodadi Central Java, in
producing levan. This bacteria displayed positive potential in producing levan based
on the appearance of slime mucoid on a sucrose medium. The optimum production
of levan was attained when the culture medium containing sucrose and peptone as
respective carbon and nitrogen sources was shaking incubated with speed of 150
o
rpm for 24 hours at 37 C. Thermal gravimetric analysisrevealed that levan
o
produced by B. licheniformis BK AG21 decomposed at 214 C. The structure of the
1 13
isolated levan was elucidated with FTIR, H and C NMR spectroscopies. Based
on the obtained spectroscopic data, the isolated levan was composed of β-(2,6)-
linkages of fructose residues.

Keywords: levan; halophilic bacteria; Bacillus licheniformis BK AG21

52
 054
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Microwave-assisted Fischer indole synthesis of

1,2,3,4- tetrahydrocarbazole catalyzed by 1,3-
dialkylimidazolium bromide ionic liquids

Ivy L. Librandoa* and Evelyn C. Creenciaa

a
Mindanao State University-Iligan Institute of Technology, Tibanga, Iligan City, 9200,
Philippines

Abstract
The research and application of green chemistry principles have led to the
development of cleaner processes. In this sense, ionic liquids (ILs) are being
researched intensively as greener alternatives to volatile organic media in a wide
range of chemical processes. Ionic liquids are salts with melting points less than
o
100 C and consist of a large organic cation combined with an organic or inorganic
anion. Thus, a series of 1,3-dialkylimidazolium bromide ionic liquids were
synthesized from commercially available 1-methylimidazole (1-MIM) and alkyl
bromides (RBr) with R being n-C4, sec-C4, tert-C4, n-C6, n-C10 and benzyl.
Synthesis was done by exposing the neat mixture of reactants to microwave
irradiations with conditions set at 100 W, 300 W, and 600 W. Moreover, the
utilization of ionic liquid as greener catalyst for the one-pot Fischer indole synthesis
of 1,2,3,4-tetrahydrocarbazole (THC) was examined. The efficiency of ionic liquids
as catalyst was evaluated in terms of product yield. Using these parameters,
Fischer indole synthesis of 1,2,3,4-tetrahydrocarbazole catalyzed by ionic liquids
was compared with other catalysts such as ionic liquids with ZnCl2 (IL+ ZnCl2) and
with ZnCl2 only. Results show pronounced catalytic activities were obtained in the
combination of ionic liquids and ZnCl2 (IL+ ZnCl2). Among the catalysts used, the 1-
decyl-3-methylimidazolium bromide ionic liquid with ZnCl2 gave an excellent yield of
94 % THC. Furthermore, the structure of the synthesized compounds was
elucidated on the basis of spectral evidences such as Fourier Transform Infrared
1
(FT-IR), H-NMR and 2D-NMR spectroscopic experiments.

Keywords: ionic liquids; 1-methylimidazole; microwave; Fischer indole synthesis;

1,2,3,4-tetrahydrocarbazole

53
 055
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of Ca2+ Ion to The Activity and Stability of

Lipase Isolated from Chromohalobacter japonicus
BK-AB18

Rukman Hertadi* and Henny Widhyastuti

Department of Chemistry, Bandung Institute of Technology, Jl Ganeca no. 10 Bandung 40132,
Indonesia

Abstract
Extracellular lipase Chromohalobacter japonicus BK-AB18 have been isolated and
fractionated with 60-70% ammonium sulfate. Native PAGE and zymography
analysis revealed a single 37 kDa lipase exist in this fraction. This enzyme exhibited
optimum activity at 45 °C and pH 9.5. The addition of various metallic ions affecting
2+
the activity of lipase and among them, Ca ion was the most prominent in
2+
enhancing its activity. In the presence of Ca ion, themostability of the enzyme was
o
improved as showed by the relatively high activity of the enzyme at 85 C. The
optimum pH in the presence of this ion turn into a wider range of value instead of a
single one, which was from 7.5 to 9.5. The enzyme also became stable against
nonpolar organic solvent (n-hexane), detergent (5 mM SDS) and hypersaline
2+
solution (8% NaCl solution). Therefore, in the presence of Ca ion, lipase isolated
from Chromohalobacter japonicus BK-AB18 is potential to be developed for
industrial applications, particularly in the industries that run their process in harsh
conditions, such as high temperature, high salinity and involving organic solvent or
surfactant.

Keywords: extracellular lipase; Chromohalobacter japonicus BK-AB18; metal ion

effect

54
 056
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Cloning of Haloacid Dehalogenase Gene from

Bacillus cereusLocal Strain with the Addition of
Restriction Sites

Idris and Enny Ratnaningsih*

Biochemistry Divison, Chemistry Study Program, Faculty of Mathematics and

Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10,
Bandung 40132, Indonesia

Abstract
Previous studies had successfully isolated and characterized the haloacid
dehalogenase gene from Bacillus cereus local strain,namely bcfd1 gene. In this
research, this gene is further sub cloned into expression vector in order to analyse
its expression.However, this gene could not be directly sub cloned because it
doesn’t have suitable restriction sites that facilitate correctorientation of cloning.
Therefore, the addition of suitable restriction sites at both end of the gene was
necessary. The research isstarted by designing specific pair of primers to amplify
the bcfd1 gene from Bacillus cereus chromosome by adding EcoRI onforward
primer and HindIII on reverse primer. The 870 bp of bcfd1 gene codes for haloacid
dehalogenase with suitable restictionsite had been successfully cloned into the
pGEM-T Easy cloning vector. The obtained recombinant clone which was screened
byampicillin resistant and ß-galactosidase activity was confirmed by size screening,
restriction analysis, and re-PCR.
.
Keywords: Bacillus cereus; cloning; haloacid dehalogenase; restriction sites.

55
 057
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of Glycerol as Carbon Source for

Biosurfactant Productionby Halophilic Bacteria
Pseudomonas stutzeri BK-AB12

Monica Putri and Rukman Hertadi*

Department of Chemistry, Bandung Institute of Technology, Jl. Ganeca 10 Bandung 40132,
Indonesia

Abstract
Biosurfactant is a surface active agents synthesized by living organism as a
secondary metabolites. Biosurfactant can besynthesized by bacteria in the limited
condition of nutritions. In this experiment, biosurfactant was synthesized and
charaterizedfrom halophilic bacteria isolated from Bledug Kuwu Mud Crater,
Pseudomonas stutzeri BK AB-12 with glycerol as carbonsource and KNO3 as
nitrogen source. The growth medium used was Luria Bertani (LB) and the
production medium was Gly-MSM with the additional of 5% salt concentration.
Biosurfactant activity was monitored with Oil Spreading Test (OST) method.The
glycerol concentration was varied from 2 to 6% to obtain the optimum condition of
bacteria in producing biosurfactant. Thehighest OST result obtained by 3% glycerol
o
concentration after the bacteria were incubated for 28 hours at 37 C and with the
aeration rate of 150 rpm, wherein the resulted oil spreading diameter was about
1.35 cm. Supernatant which contain thebiosurfactant gave emulsification index
(EI24) 53.33% and worked optimally at pH 12 to 14, 15% NaCl concentration, and
o
temperature until 60 C. CTAB result gave blue zone on the Blue Plate Agar
indicated that the biosurfactant is anionic type.Biosurfactant gave CMC at 48.44
mg/L. FTIR characterization exhibited high similarity of the biosurfactant spectrum
to that ofrhamnolipid standard.

Keywords: halophilic bacteria, glycerol, Oil Spreading Test, Pseudomonas stutzeri

BK AB-12

56
 058
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis and Characterization of Carbon

Nanospheres Using Cooking Palm Oil as Natural
Precursors onto Activated Carbon Support

Hans Kristiantoa, Cahyadi Dwi Putraa, Arenst Andreas Ariea*,

Martin Halimb, and Joong Kee Leeb
a
Department of Chemical Engineering, Faculty of Industrial Technology, Parahyangan
Catholic University, Ciumbuleuit 94 Bandung 40141 Indonesia
b
Center of Energy Convergence, Korea Institute of Science and Technology, Hwarangno 14-
gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea

Abstract
Since its discovery, carbon nanomaterials, such as nanotubes, nanohorns,
nanoribbons and so forth, have gained a lot of attention among researcher. Carbon
nanospheres (CNSs) are one of novel carbon nanostructures which are known for
its potential application in many fields, such as electrodes in supercapacitors and
lithium ion batteries, adsorbents, and catalyst support. In this study, Carbon
Nanospheres (CNSs) was synthesized using simple pyrolysis process under
nitrogen atmosphere. Cooking palm oil was used as natural and renewable carbon
precursors, ferrocene as catalyst and activated carbon as support. Ferrocene was
used at various concentrations (2.5, 5.0, and 7.5g/100mL precursor). The effect of
catalyst amount to the morphology and characteristics of CNSs obtained was
observed by x-ray diffraction (XRD), BET surface area analysis, scanning electron
microscope (SEM), and energy dispersive x-ray spectroscopy (EDS). Peak of C
(002) and (100) was observed in XRD spectra of all catalyst concentrations, while
there was decrease of surface area in all samples, indicating the covering of
activated carbon pores by Fe catalyst and CNSs. With increase of the catalyst
concentration, there was also increase in content of Fe and O. The most dense
CNSs structure was obtained by pyrolyzing palm oil onto activated carbon support
with ferrocene concentration 7.5g/100mL, with average diameter of 87.5nm.

Keywords: Carbon nanospheres (CNSs); ferrocene: pyrolisis; activated carbon

57
 059
International Symposium on Applied Chemistry 2015
(ISAC 2015)

The Preliminary Study of The Dye Extraction from

The Avocado Seed Using Ultrasonic Assisted
Extraction

Ariestya Arlene Aa*, Anastasia Prima Ka, Lidya Utamaa, Airin

Anggraini Sa
a
Jurusan Teknik Kimia, Fakultas Teknologi Industri, Universitas Katolik Parahyangan Jl.
Ciumbuleuit No. 94 Bandung-40141

Abstract
Avocado (Persea americana Mill) is the plant produced in the tropical countries, for instance
Indonesia, which is the third largest avocado producer in the world. The crushed avocado
seed can produce natural orange dye and also has potential to be natural food dye. The
extraction using ultrasonic wave (ultrasonic assisted extraction – UAE) is proven to give the
better result than the maceration extraction. The aim of this research is to study the influence
of the temperature and the feed to solvent ratio towards the yield, the dye color intensity and
the phenolic content using the two factor factorial design in the avocado seed UAE. The
avocado seed was crushed and blended with aquades as much as 70% from the seed mass.
The slurry was incubated at 24 ºC for 35 minutes. Afterwards, the slurry was extracted using
sonicator with methanol as solvent. The variations of the research were the extraction
temperature and ratio of feed to solvent, that are 30, 40, 50, 60, 70 ºC and 1:3, 1:6, 1:9, 1:12,
1:15 respectively. The extract was filtrated from the raffinate using the Buchner then the
solvent was separated using the vacuum evaporator at 3,37 mbar and the waterbath
temperature of 50 ºC. The analysis of the color intensity and the phenolic content were
conducted by means of the absorbance measurement using the UV-Vis Spectrophotometer. In
addition, the dye was also analyzed with GC-MS to get the information about the compound
which causes the orange appearance. Based on the variance analysis of the experimental
design, it can be concluded that the temperature and the feed to solvent ratio do not affect the
yield, the phenolic content and the color intensity. However, there is an interaction of the two
factors towards those three responses. The highest yield of the results is 22.6 % obtained
from temperature extraction 70 ºC and feed to solvent ratio 1:12. The furfural compound is
found in the GS-MS analysis and assumed to develop the orange color of the dye.

Keywords: avocado; extraction; dye; seed; ultrasonic

58
 060
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Isolation and Characterization of Organic-Solvent

Stable Protease Isolated by Pseudomonas
stutzeri BK AB-12

Dian Siti S, Ihsanawati, Rukman Hertadi*

Department of Chemistry Bandung Institute of Technology, Bandung 40116 Indonesia

Abstract
In this study, proteases have been isolated from halophilic bacteria, Pseudomonas
stutzeri BK AB-12. The bacteria produced proteases as exhibited by the
appearance of clear zones resulted from casein hydrolysis. Based on the protease
specific activity, the highest activity of protease has been collected in the fraction of
7080% ammonium sulfate. Protease in this fraction exhibited highest catalytic
performance at pH 8.0 and 55 °C. The activity of protease was enhanced by the
addition of several metal ions and among those, Fe3+ ion was the most significant
in improving the catalytic performance of the enzyme. The addition of Fe3+ ion also
resulted in a shifting of the optimum pH from 8 to 9 and the optimum temperature
from 55 to 60 °C. Protease in this fraction has been verified not included among
metallo-, cysteine-, or serine proteases because it was not inhibited by EDTA, β-
mercaptoethanol, and PMSF. Proteases of this fraction was sensitive to ionic
strength, where the highest activity was observed at the concentrations of 2.5 M
NaCl. In addition, this enzyme also stable in various organic solvent and its activity
even significantly enhanced in polar organic solvent as n-propanol and nonpolar
solvent as n-hexane.

Keywords: protease; Pseudomonas stutzeri; halophiles

59
 061
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Performance of M-Silicate and M-Polystyrene

Sulfonate in Extraction of Carotenoids From
Crude Palm Oil

Nimpan Banguna*, Seribima Sembiringa, Nabila Karina Putria,

Justaman Arifin Karo Karob
a
Laboratory of Inorganic Chemistry, Faculty of Mathematics and Natural Sciences, University
of Sumatera Utara, Jln. Bioteknologi no.1 Medan 20155, Indonesia
b
Agency of Industrial Research and Standarization Medan 20215, Indonesia

Abstract
Four polar adsorbents M-Silicates (M = Mg, Ca) and M-PSS (M = Mg, Ca; PSS =
Polystyrene sulfonate) have been used to adsorb carotenoids from crude palm oil
(CPO). The carotenoids from the adsorbents were desorbed by ethanol in a soxhlet
extractor and collected in toluene which was placed together with ethanol in the
soxhlet flask. The concentrations of the carotenoids in the aliquots, which were
taken every 10, 20, 30, 40, 50 and 60 cycles from the toluene extract, was
determined by PORIM method. It was found that generally the maximum
carotenoids were obtained from the aliquot with 50 cycles. The extraction processes
using Mg-silicate, Ca-silicate, Mg-PSS, Ca-PSS will produces extract-A, B, C and
D. The carotenoids extracted in extract-A, B, C and D was 71.34%, 67.01 %, 71.34
% and 90.77% respectively. All the extracts still contain some triglyceride and free
fatty acids. In order to reduce the triglyceride and free fatty acid content in the
extracts, extract-A, B, C and D were then adsorb by reused M-Silicates and M-PSS
adsorbents. The maximum carotenoids was found in extract-D which had been
adsorb by Ca-silicate which was 91.30 %, and the least was found in extract-C
which have been adsorb by Mg-PSS which was 44.31 %.

Keywords: carotenoids; crude palm oil; adsorbents; desorption

60
 062
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Docking and Molecular Dynamics Simulation of

CarbonicAnhydrase II Inhibitors from Phenolic
and Flavonoid Group

Aditama, R.a, Mujahidin, D.b, Maolana Syah, Y.b ,Hertadi

R.a*
a
Biochemistry Research Group Faculty of Mathematics and Natural Sciences, Institut
Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia
b
Organic Research Group Faculty of Mathematics and Natural Sciences, Institut
Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia

Abstract
Carbonic Anhydrase II (CAII) has role in pH regulation, water transport and
hydration of CO2. Besides its functions, CAII also related to many diseases,
including glaucoma, tumors, epilepsy, diabetes and osteopetrosis. Various inhibitor
of CAII have been developed and commercialized as drug. Recent development of
CAII inhibitor leads to obtaining a novel inhibitor based from natural product. This
research aim to screen potent inhibitors from phenolic and flavonoid group with
computational approach. The screening of natural products compounds were
performed by docking simulation. Binding pose from docking then analyzed with
molecular dynamics simulation to evaluate the stability of interaction. Docking
results predicted that fisetin (Fic) and 6-(3,4-dihydroxyphenyl)-5,6,7,8-
tetrahydronaphthalene-1,3,7-triol (Afr3) has potency to be used for CAII inhibitor.

Keywords: docking; flavonoid; carbonic anhydrase inhibitor; molecular dynamics

simulation

61
 063
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Esterification of Free Fatty Acid on Palm Fatty

Acid Distillate using Activated Carbon Catalysts
Generated from Coconut Shell

Arif Hidayata,b, Rochmadib, Karna Wijayac, Arief Budimanb,d

a,b
Chemical Engineering Department, Indonesia Islamic University, Jalan Kaliurang km 14,5
Ngemplak Sleman Yogyakarta Indonesia 55584
b
Chemical Engineering Department,Gadjah Mada, Jalan Grafika Bulaksumur Sleman
Yogyakarta Indonesia 55281
c
Chemistry Department,Gadjah Mada, Sekip Utara Bulaksumur Sleman Yogyakarta Indonesia
55281
d
Center for Energy Studies, Gadjah Mada University, Sekip K1A, Yogyakarta 55281,
Indonesia.

Abstract
In the last few years, biodiesel has emerged as one of the most potential renewable energy to
replace current petrol-derived diesel. It is a renewable, biodegradable and non-toxic fuel which
can be easily produced through esterification of triglycerides (vegetable oils or animal fats) or
esterification of free fatty acids (FFAs) with methanol. However, current commercial usage of
refined vegetable oils for biodiesel production is impractical and uneconomical due to high
feedstock cost and priority as food resources. Low-grade oil, can be a better alternative;
however, the high free fatty acids (FFA) content has become the main drawback for this
potential feedstock. Solid acid catalysts offer significant advantages of eliminating separation,
corrosion, toxicity and environmental problems. Recently, a new strategy of preparing novel
carbon-based solid acids has been developed. In this research, the esterification reactions of
Palm Fatty Acid Disttillate (PFAD) with methanol, using carbon based solid catalyst from
coconut shell activated carbon as catalyst, were studied. The ester preparation involved an
esterification reaction, followed by purification. In this study, the activated carbon catalysts
were synthesized by sulfonating the activated carbon using concentrated sulfuric acid. The
physico-characteristics and acid site densities were analyzed by Nitrogen gas adsorption, FT-
IR, X-ray fluorescent (XRF), and acid-base back titration methods. The effects of the molar
ratio of methanol to PFAD (6:1 to 12:1), the amount of catalyst (1-10%wt. PFAD), and the
reaction temperature (40, 50 and 60oC) were studied for the conversion of FFA to optimize the
reaction conditions. The optimal conditions were an methanol to PFAD molar ratio of 12:1, the
amount of catalyst of 10% wt. PFAD, and reaction temperature of 60oC.

Keywords: biodiesel, palm fatty acid distillate, biomass waste, esterification

62
 064
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Antibacterial and Antioxidant Activity Evaluation

of 1,3-Diaryl-prop-2-en-1-one Derivatives

Melanny Ika Sulistyowatya*, Kholis Amalia Nofiantia, Juni

Ekowatia, Galih Satrio Putraa, Tri Widiandania, Tutuk Budiatia
a
Faculty of Pharmacy, Universitas Airlangga, Jln. Dharmawangsa Dalam Selatan
Surabaya,60286, Indonesia

Abstract
Some 1,3-diaryl-propenone derivatives had been synthesized by a conventional
Claisen-Schmidt condensation in the previous experiment. This study purposed to
examine their antibacterial activity against Staphylococcus aureus, Escherichia coli
and Candida albican by using agar diffusion susceptibility method. The tested
compounds were also screened for antioxidant activity by DPPH method. The
results of antibacterial activity showed that the tested compounds were inactive
toward Escherichia coli, but still had modest ability to inhibit Staphylococcus aureus
and Candida albican, compared to standard drugs. While the results of antioxidant
activity disclosed that the compound with hydroxyl groups which possessed
antioxidant ability (16.36 %), but not the others.

Keywords: Antibacterial activity; Antioxidant activity; DPPH; 1,3-diaryl-propenone

63
 066
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Characteristics and Bond Performance of Wood

Adhesive Made from Natural Rubber Latex and
Alkaline Pretreatment Lignin

Euis Hermiatia*, M. Adly Rahandi Lubisa, Lucky Risantoa, R.

Permana Budi Laksanaa, Lukmanul Hakim Zainib
a
Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km
46, Cibinong, Bogor 16911, Indonesia
b
Department of Forest Product Technology, Faculty of Forestry, Bogor Agriculture University,
Kampus IPB Darmaga, Bogor, Indonesia

Abstract
The aim of this study was to characterize an aqueous polymer isocyanate (API)
type adhesive made from natural rubber latex (NRL) and lignin as base polymers,
and to evaluate bond performance of the adhesive as laminated wood adhesive.
The base polymers of the adhesive were prepared by blending NRL, polyvinyl
alcohol (PVA), and lignin isolated from black liquor of alkaline pretreatment of oil
palm empty fruit bunch (OPEFB) and sugarcane bagasse (SB) with compositions of
25/25/0, 25/20/5, 25/15/10, 25/10/15, 25/5/20, and 25/0/25 (w/w/w). The isocyanate
crosslinker was added at the level of 15% of the weight of base polymer. The glass
transition temperature (Tg), heat degradation, and the homogenity of the adhesive
blend were analyzed. The adhesive was used for producing laminated wood (20×8
cm2). Results showed that the addition of lignin in the base polymer blends of API
adhesive did not significantly affect the Tg of the adhesives. However, it affected the
thermal decomposition and bond performance of the adhesives. There were more
residues and less homogenous adhesive solution due to the addition of lignin in the
base polyemr blends of API adhesives. The addition of lignin in the base polymer
blends caused significant decrease in bond performance of the adhesive applied in
glue laminated wood.

Keywords: Wood adhesive; aqueous polymer isocyanate; natural rubber; lignin;

isocyanate crosslinker; laminated wood

64
 067
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Alpha Glucosidase Inhibitory Activity of Aqueous

Extract ofCymbopogon citratus, Musa
paradisiaca, and Allium cepa L. var.aggregatum

Gregorius Herdiazto Adiyogaa, Irvan Kartawiriab, Filiana

Santosoa, and Maria DewiPuspitasari Tirtaningtyas Gunawan-
Puteria*
a
Department of Food Technology, Life Sciences and Technology, Swiss German University,
EduTown-BSD City 15339
b
Department of Pharmaceutical Engineering, Life Sciences and Technology, Swiss German
Uniiversity, EduTown-BSD City 15339

Abstract
Three tested Indonesian herbal plants were found to have potential of containing
high alpha glucosidase enzymeinhibitors. These plants were Cymbopogon citratus,
Musa paradisiaca, andAllium cepa L. var. aggregatum. Theperformance of AGIs of
each plants were tested using water as the extracting solvent, which resulted 100%
inhibition activity at the concentration of 500 µg/ ml for C. citratus, M. paradisiaca,
and A. cepa L. var. aggregatumrespectively. Cytotoxicity of each plants were tested
using brines shrimp lethality test or BSLT to confirm thebioactivity of each plant,
which then the results were 36.38 µg/ ml, 6.99 µg/ ml, and 1.32 µg/ ml for
Cymbopogoncitratus, Musa paradisiaca, and Allium cepa L. var. aggregatum
respectively. Direct infusion method of the driedplant samples into water with three
different temperatures, 60 °C, 70 °C, and 80 °C with 10, 20, and 30 minutes
ofinfusion time. This direct infusion method is able to achieve AGIs inhibition
activities ranging from 63% until100%. The highest AGIs performance was obtained
from M. paradisiaca peels with 100% inhibition activity at theinfusion temperature of
60 °C at 20 and 30 minutes infusion time, at 70 °C with 10, 20, and 30 minutes of
infusion
time and at 80 °C with 10 and 20 minutes of infusion time.

Keywords: Alpha-glucosidase inhibitor; Cymbopogon citratus, Musa paradisiaca,

Allium cepa L. var. aggregatum; direct infusion.

65
 068
International Symposium on Applied Chemistry 2015
(ISAC 2015)

66
 069
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effects of Ionic Liquid as Phase Modifier in

PMMA/ENR 50 Blend Electrolyte

Lina Nadzirah Salahuddina,*, Famiza Abdul Latifa

aFaculty of Applied Sciences, UniversitiTeknologi MARA, 40450 Shah Alam, Selangor,
Malaysia

Abstract
Previously the addition of Epoxide Natural Rubber(ENR 50) has improved the
brittleness of Polymethaclyrate(PMMA) film electrolyte, hence enhance the ionic
conductivity of the film. However the PMMA/ENR 50 blend film was not
homogenous in which phase separation can be observed. Though several additives
such as plasticizers and fillers have been introduced. They were found not
compatible with the blend system. Therefore, in this study, 1-ethyl-3-
methylimidazolium bis(trifluoromethylsulfonyl) imide, ionic liquid was introduced to
improve the homogenity of the blend. Interestingly it was found that the phase
seperation between the polymers were almost diminishing indicating that the
homogenity of the blending has been improved. The homogenity was determined
using Differential Scanning Calorimetry(DSC), Thermogavimetric Analysis(TGA),
Fourier Transform Infrared spectroscopy(FTIR) and Optical Microscope(OM)
studies. The ionic conductivity were studied using Impedance Spectroscopy (EIS).

Keywords: polymer electrolyte, PMMA, ENR50, ionic liquid, EMIM(Tf2N), EMIMIm

67
 070
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Production of Human Milk Fat Replacement Rich

of 1,3-dioleoyl-2-palmitoilglycerol From
Enzymatic Interesterification Tripalmitin, Ethyl
Oleate And Mixture of VCO, Soybean Oil And
Fish Oil

Sadiah Djajasoepena*, O. Suprijana, Saadah Diana R., Uji

Pratomo, and Sisca Puspitasari
Department of Chemistry, Padjadjaran University, Jl. Raya Jatinangor, Km. 21, Sumedang,
Indonesia.

Abstract
Human Milk is naturally the only source of food for infant in their early life. It
contains 2-6 % lipid which provides about 50% of the total energy needed by the
infant. Human milk fat (HMF) mainly as TAG with the specific fatty acid composition,
palmitic acid (C16:0) (20-25%), which is primary located at sn-2 of glycerol bonds
(70%) and oleic acid (C18:1), located at sn-1,3 (35 %). HMF also provide fatty acids
such as linoleic acid, linolenic acid, EPA, DHA and lauric acid that are very
important for infant. The purposes of this research are to synthesize of 1,3-dioleoyl-
2-palmitoilglycerol (OPO) and to determine the best composition of OPO, VCO,
soybean oil and fish oil for HMFS production for infant formula. Interesterification of
tripalmitin and ethyl oleate using immobilized lipase from Rhizomucor miehei
(Lipozym RM IM) were used to synthesize of OPO. Interesterification product of
mixed VCO, soybean oil and fish oil that are source of lauric acid, linoleic acid, α-
linolenic, EPA and DHA, were formulated in mass ratio (58:20:20:2) and
(70:18:10:2) for obtaining HMFS which have fatty acids composition similar or close
to HMF. Composition of fatty acids from product were analyzed by GCMS. From
this research, were obtained HMFS containing palmitic acid as much as 28.89%
where 84.49% of that are located at sn-2 while sn-1,3 position are dominated by
oleic acid as much as 55.11% from the total 38.7% and 70:18:10:2 w/w is the best
composition of interesterification product, VCO, soybean oil and fish oil to obtain
HMFS similar to HMF.

Keywords: Human milk fat replacement; OPO; Lipozym RM IM

68
 071
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Structural Analysis of Halophilic Lipases Isolated

FromBledug Kuwu Mud Crater, Purwodadi,
Indonesia:a Structural Bioinformatics study

Mukhammad Asy’aria, Pingkan Aditiawatib, Akhmalokaa and

Rukman Hertadia*
a
Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Institut
Teknologi Bandung, Jl. Ganesha no.10 Bandung 40132
b
Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut
Teknologi Bandung, Jl. Ganesha no.10 Bandung, 40132

Abstract
We have cloned lipase genes isolated from six halophilic bacteria isolated from
Bledug Kuwu mud crater,Purwodadi, Indonesia. In the present study, we carried out
structural bioinformatics analysis to estimate thestructural properties of the cloned
gene products. In this study, crystal structures of several proteins originated
fromnonhalophiles, moderate and extreme halophiles were used as references.
Moderate and extreme halophilic proteinswere grouped into OSMOL and SALT-IN
proteins, respectively, which referred to the adaptation strategy ofmoderate and
extreme halophiles against external osmotic pressure exerted by hypersaline
solution in their habitats.All structural bioinformatics analysis presented here were
carried out by evaluating the relative difference of variousanalysed parameters
between all halophiles proteins including the references and the nonhalophiles
ones. Lipasesamples from the six Bledug Kuwu bacterial isolates displayed different
profiles in amino acid composition,secondary structures, solvent accessible surface
areas and radius of gyrations compare to those of all the referencesstructures
including OSMOL and SALT-IN proteins. These differences may be correlated to
the unique halophileshabitat of all six bacteria isolates, which is the salty mud crater
situated at the mainland and far from the sea.Therefore, based on this analysis we
can expect that the biochemical and structural property of those six lipasesamples
were unique as well.

Keywords: bioinformatic, halophilic, lipase, halophilic, Bledug Kuwu, 3-dimensional

structure

69
 072
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Study of Softening Hard Water Using Pistacia

Vera Shell As Adsorbent for Calcium And
Magnesium Removal

Uji Pratomo*, Anni Anggraeni, Rubianto A. Lubis and

Allyn Pramudya
Department of Chemistry, Padjadjaran University, Jl. Raya Jatinangor, Km. 21, Sumedang,
Indonesia

Abstract
In Indonesia, the use of ground water is increases. It contains minerals, especially
calcium and magnesium, at high concentration. This causes problems such as the
crust on household appliances and higher use of soap for washing. Shell of Pistacia
vera l. were study as an adsorbent of Ca and Mg in the matrix artificial hard water.
Pistacia v. shell were dried and crushed into powder form. A batch experiment with
various parameters was used in this research. Optimum adsorption conditions is at
pH 8 and the weight of Pistacia v. shell is 1.5 g. Adsorption capacity of Ca and Mg,
respectively 2.41 and 2.19 mg/g. Based on the research results, it can be
concluded that the Pistacia v. shell is a very potential as a biodegradable, easy and
inexpensive bioadsorbent to adsorb Ca and Mg.

Keywords: Pistacia vera L; Bioadsorption; Calcium; Magnesium

70
 073
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Aqueous-Methanol Extract of Gracilaria verrucosa

InducesCytochrome c Release From Mitochondria

Mona Fitriaa, Fida Madayanti Warganegaraa*

a
Chemistry Department, Faculty of Mathematic and Natural Science, Institut Teknologi
Bandung, Jl. Ganesa 10, Bandung 40132, Indonesia

Abstract
Seaweeds is one of many sources of natural bioactive compounds that can be
utilized in the fields of pharmaceuticals and health.However, research on utilization
of the seaweed’s bioactive compounds from Indonesia is still very limited.
Therefore, theobjective of this research is to study the ability to induce apoptosis of
the extracts of Gracilaria verrucosa harvested from SayangHeulang, a beach in
Pameungpeuk, Garut, West Java. The study begins with bioactive compounds
extraction using chloroformp.a, methanol p.a and 50 mM phosphate buffer pH 7.6
with a ratio 2:1:0.8, resulting crude aqueous-methanol and chloroformextracts. G.
verrucosa produced 62.73% (w/w) crude aqueous-methanol extract and 1.07%
(w/w) chloroform extract.Mitochondria for apoptosis test were isolated from rat liver.
The treatment of 10% v/v crude aqueous-methanol extract from G.verrucosa for five
hours at 37 oC toward mitochondrial suspension leads to formation of mitochondrial
aggregates and study ofthe released of cytochrome c showed that those treatment
released 8.7 µM cytochrome c which has equal to 11.4% ofmitoxantrone 500 µM
activity. In conclusion, our results indicate that aqueous-methanol extract of G.
verrucosa able to induceapoptosis through mitochondrial pathway involving
cytochrome c release from mitochondria.
.
Keywords: Seaweeds, apoptosis, cytochrome c, mitochondria

71
 074
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Microwave-assisted Fischer indole synthesis of

1,2,3,4-tetrahydrocarbazole using pyridinium-
based ionic liquids

Ramon M. Eduque, Jr.a,* and Evelyn C. Creenciaa

a
Mindanao State University - Iligan Institute of technology, Tibanga, Iligan City 9200,
Philippines

Abstract
Microwave-assisted organic synthesis using ionic liquids is believed to enhance
efficiency of the reaction leading to a cleaner method, higher product yields and
shorter reaction times. Six pyridinium-based ionic liquids were prepared from
2-methylpyridine and a series of alkyl bromides (R : n-C4, sec-C4, t-C4, C6, C8 and
Bn) by oil bath heating at 80, 90, 100 and 110 °C. The ionic liquids product yield
efficiency has direct relationship with heating temperatures affording highest yields
at 110 °C of 71.15%, 8.20%, 45.96%, 57.75%, 48.02% and 80.45% for each
respective alkyl bromides. The catalytic activities of the synthesized ionic liquids in
the Fischer indole synthesis of THC were examined. The highest yield of 67.82% is
found to be less satisfactory compared to the use of the Lewis acid standard, ZnCl2,
which gave 79.89%. On the other hand, the use of the pyridinium-based ionic
liquids in conjunction with ZnCl2 afforded better yields than individual test catalysts
resulting to 89.66% of the THC. Structural effects such as branching and chain
length of the alkyl bromides for the synthesis of ionic liquids and the THC have
negative effects on the product yields.
.
Keywords: microwave-assisted synthesis; ionic liquids; Fischer indole

72
 075
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Characterization of Iron Metal Corrosion in Liquid

Smoke Coagulant

Afrizal Vachlepi1* dan Didin Suwardin1

1
Sembawa Reserach Center- Indonesian Rubber Research Institute, Jl. Raya Palembang-
Betung Km.29 Po. Box 1127 Palembang South Sumatera 30001, Indonesia

Abstract
Latex coagulant is one of important factors on natural rubber processing because it
will determine the quality of rubber end products. One of the coagulants that
produce good quality natural rubber is liquid smoke. The use of liquid smoke as
coagulant is thought to cause corrosion in processing equipment commonly made
of iron or steel. The objectives of this study were to identify and to study the
characteristic of iron metal corrosion in the solution of liquid smoke coagulant. The
treatment of liquid smoke as corrosion media consisted of 100% pure liquid smoke
(AC100), 10% pure liquid smoke (AC10), 100% liquid smoke formula (FA100) and
10% liquid smoke formula (FA10). The method used was weight loss method with
the planned interval test. The removal of corrosion products on the metal specimen
was done by pickling method based on ASTM G1-81. The results showed that
corrosion rate of the liquid smoke and its formula was around 0.05-0.23 mm per
years. Corrosion resistance of iron metal in liquid smoke coagulant was categorized
as good level. This analysis indicated that liquid smoke coagulant and its formula
has very low corrosivity level. The visualization with 10x magnification showed that
the corrosion attack was clearly visible on the iron metal surface.

Keywords: liquid smoke, iron metal and corrosion

73
 076
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Potential of Oil Palm Empty Fruit Bunch (EFB) as

Media for Oyster Mushroom, Pleurotus ostreatus
Cultivation

Lin Marlinaa*, Setiarti Sukotjob

a
Department of Chemical Engineering-ITI, Jl. Raya Serpong, Tangerang 15320, Indonesia
b
Department of Agro-Industrial Technology-ITI, Jl. Raya Serpong, Tangerang 15320, Indonesia

Abstract
Oil palm is one of the most important oil crops in Indonesia. Oil palm empty fruit
bunch (EFB) could serve as an alternative and cheap media for edible mushroom
cultivation. This study investigated the possibility of using EFB for cultivating edible
mushroom Peurotus ostreatus. The objectives of this research are to formulate best
media for Pleurotus ostreatus cultivation, to give alternative for palm oil Industries to
use EFB for mushroom cultivation, and to support Indonesian government policy to
increase food security. The experiment, which covered the media preparation,
inoculation, incubation and harvesting used different EFB media composition.
Media formulation consisted of EFB, rice bran, CaC2, mineral fertilizer (TSP 12-12-
12), and sawdust. The starter was added to the sterilized media. The experiment
was done in three replicates. Data were collected on mycellium growth and weight
of mushroom yield. Variation 3 showed the fastest growth of mycellium among other
variations. Variation 1 yield the highest weight with 289.7 g in the first harvest.

Keywords: empty fruit bunch, oyster mushroom, Peurotus ostreatus, media,

cultivation.

74
 077
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Impact Strength and Thermal Degradation of

Waste Polypropylene (wPP)/Oil Palm Empty Fruit
Bunch (OPEFB) Composites: Effect of Maleic
Anhydride -g-polypropylene (MAPP) Addition

Halimatuddahliana Nasutiona*, Setiaty Pandiaa, Maulidaa,

Mersi Suriani Sinagaa
a
Department of Chemical Engineering, Universitas Sumatera Utara, Jalan Almamater, Kampus
USU Medan 20155, North Sumatra, Indonesia

Abstract
The effect of maleic anhydride-g-polypropylene (MAPP) addition on the impact
strength and thermal degradation of waste polypropylene (wPP) / oil palm empty
fruit bunch (OPEFB) composites was investigated. Several contents of MAPP viz. 2,
4, 6, 8 weight percent (wt%) were prepared. Corresponding wPP/OPEFB composite
without MAPP addition was also made for comparison. The OPEFB composition
was fixed at 30 wt%, while wPP was 70 wt% and reduced correspondingly on the
addition of MAPP. Composites were prepared in extruder with temperature of
190oC and followed with compression molded at the same temperature.
Composites were evaluated using impact tester machine and thermogravimetric
analysis (TGA) to conform the impact strength and thermal degradation,
respectively. Results indicated an improvement in impact strength was obtained on
the addition of MAPP up to 8 wt%. The results were also confirmed that the
incorporation of MAPP has increased degradation temperature of wPP/OPEFB
composites.

Keywords: waste polypropylene, oil palm empty fruit bunch, maleic anhydride -g-
polypropylene, impact strength, thermal degradation

75
 078
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Stability Study of Betacyanin Extract from Red

Dragon Fruit (Hylocereus polyrhizus) Peels

Sri Priatnia*, Aulia Praditab

a
Research Centre for Chemistry – Indonesian Institute of Sciences, Jl. Sangkuriang Bandung,
Indonesia 40135
b
Chemistry Department – Indonesian Education Universit

Abstract
Dragon fruit (Hylocereus polyrhizus) or buah naga is one of the tropical fruits under
the cactus family, Cactaceae. Dragon fruit becoming popular in Indonesia due to its
attractive color, sweet, juicy pleasant taste and also the plant is beautiful. The peels
of red dragon fruit is the potential sources of betacyanins which responsible for the
red-violet color. The objective of this study was to evaluate the stability of
betacyanin extract from red dragon fruit peels. In this study, the betacyanin extract
from the peels of red dragon fruit was extracted by methanol and water. The
stability of this pigment was evaluated by monitored the effects of storage time and
pH by spectrophotometer at wavelength 538 nm. This study was also investigated
the kinetics degradation of betacyanin extract using the accelerated reaction
method. Results analysis of red dragon fruit peels extract shown that for five hours
in room temperature, betacyanin content in methanol extract was decreased about
10.44%, meanwhile betacyanin content in water extract was decreased about
22.58%. Betacyanin content was obtained from peels which extracted by methanol
pH 5 (515.20 µg/100 g) higher than betacyanin content in water pH 5 (491.16
µg/100 g). Arhenius data showed that betacyanin extract in water follow the first-
order kinetic model with its half life time (t ½) at 25oC was 23 hours and 90%-shelf
life was 76 hours.
.
Keywords:betacyanin, dragon fruit, stability, Arrhenius

76
 079
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Evaluation of Radiocesium Sorption in Some

Materials as Safety Barrier Systemof Radioactive
Waste Disposal Demonstration Facility

Budi Setiawana , Elfira Mayasarib, Shely Meidhikab

a
Center for Radioactive Waste Technology-BATAN, Kawasan PUSPIPTEK Bld 71, 3rd Floor,
Serpong-Tangerang 15310, Indonesia
b
Dept. of Chemistry, Grad. School, Fac. Of Math. And Science, Andalas University, Limau
Manis-Padang 25163, Indonesia

Abstract
Sorption characteristic study of the prospective barrier materials will be used as
safety barrier system on the disposal facilities such as bentonite, kaolinite, and
quartz sandy has been done. Such barrier materials were contacted against 137Cs
with affected parameters were the functions of contact time, concentration of CsCl,
ionic strength of solution, and the sorption isotherm, and all the experiments were
done in batch method. Objective of the research is to obtain a specific sorption
character data of radiocesium into some prospective barrier materials for
radioactive waste disposal demonstration facility at Serpong. The initial
concentration of CsCl being used was 10-8 M, and 0.1, 0.5, 1.0 M NaCl and also
10-8-10-4 M CsCl for experiments of the effects of shaking time, ionic strength and
concentration of CsCl in the solution, respectively. Sorption quantification of 137Cs
was predicted through their distribution coefficient (Kd). Equilibrium state were
reached after 7, 5 days and 24 hours shaking time with Kd values 17000; 1100 and
130 mL/g for bentonite, kaolinite and quartz sandy samples, respectively.
Increasing of NaCl and CsCl concentrations in solution caused Kd values
decreased due to the competition between Na and Cs ions when interacting with
the sample, and also due to the limited sorption capacity of the sample to 137Cs.

Keywords: Radwaste disposal demonstration facility; safety system; barrier

137
materials; Cs; sorption

77
 080
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Identification of LL and meso-diaminopimelic acid

(DAP) ofActinomycete strains and Its Secondary
Metabolite Production

Desak Gede Sri Andayani, Agustine S., Yetti Mulyati I., Puspa
Dewi L., Hakiki M.
Indonesian Institute of Sciences (LIPI), Research Center for Chemistry
Jl. Cisitu-Sangkuriang, Bandung 40135, West Java, Indonesia

Abstract
Streptomyces and Nocardia is a genus of actinomycetes group which has some
similar characteristic. Although all of them have the ability to produce antibiotics but
almost of Nocardia is pathogenic. The objective of this study is analyzed the two
strains ofactinomycetes and determine the optimum conditions of production of
secondary metabolites using Streptomyces sp.Identification to distinguish the two
strains (TP2 and TP5) by analyzing LL and meso-DAP on their cell walls.
Optimization ofthe fermentation process using one factor at one time (OFAT)
0
method that conducted at temperature of 26-40 C, pH 5-8 and120-180 rpm for 48
hours in a fermentor working volume 1 L. Antibacterial activity test performed by the
agar diffusion methodagainst resistant pathogenic bacteria. The results showed
Streptomyces sp. TP2, contain any form of LL-DAP and Nocardia sp.TP5, and
contain any form of meso-DAP as its main component. Optimization of antibiotic
production is reached afterfermentation be held for 42 hours at a temperature of 30
0C, pH 7 and 180 rpm with antibacterial activity against
methicillinsensitiveStaphylococcus aureus, methicillin resistant Staphylococcus
aureus, methicillin resistant coagulase negativeStaphylococcus, vancomycin
resistant Enterococcus, and Escherichia coli.

Keywords: LL and Meso-DAP, actinomycetes, fermentation, antibacterial test

78
 081
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Encapsulation of Gluten

Husniatia*, Etik Mardliyatib, Nofa Mardia Ningsih Kaswatic

a
Center for Research and Standardization of Industry Bandar Lampung, Jl.. By Pass
Soekarno-Hatta Km.1 Rajabasa, Bandar Lampung, 35114, Indonesia
b
Agency for the Assessment and Application Technology, Puspiptek Serpong. Indonesia
c
Nano Center Indonesia, Puspiptek Serpong. Indonesia

Abstract
Gluten is a protein contained in wheat, which gives elasticity and tensile strength
when making dough. However these properties may be lost during food processing
because of its heat sensitivity. The aim of this research is to encapsulate the gluten
for retaining the function of gluten even at high temperature when processed. The
encapsulation was examined for mixtures of maltodextrin with gum arabic or with
casein by using either spray drying or oven drying. It was found that the mixture of
maltodextrin with gum arabic (MD:GA) is more suitable for an encapsulating
material than that with casein (MD:C) and the spray drying gives the better result in
comparison with the oven drying. The optimum condition of the encapsulation was
the mixture at MD:GA ratio 2:1 and the spray drying at 170 °C. The obtained gluten
sample showed slowly adhesiveness, uniformed particles, and easy flow criteria
when dissolved in water.

Keywords: gluten; encapsulation; maltodextrin; spray drying.

79
 082
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Comparison between GC-TCD and GC-FID for the

determination of propane in gas mixture

Harry Budimana, Nuryatinia, Oman Zuasa

a
Gas Analysis Laboratory (GasAL), Research Center for Chemistry, Indonesian Institute of
Sciences (LIPI), Kawasan PUSPIPTEK, Serpong, Banten, 15314, Indonesia

Abstract
A method comparison between gas chromatography-thermal conductivity detector
(GC-TCD) and chromatography-flame ionization detector (GC-FID) for the analysis
of propane (C3H8) are reported in this study. To achieve the study purpose for
producing valid and reliable result, the method comparison process was conducted
by investigating several keys ofanalytical parameterof both GC-TCD and GC-FID
methodincludingmethod sensitivity, precision, accuracy, and method
linearity.Investigation of theGC’s parameter wereconducted by analyzingtheC3H8(%
mol/mol) in nitrogen (N2) balance (known as C3H8certified reference material). The
results show that the GC-FIDmethodwas found to display moresensitive, precise,
and accurate than GC-TCD method for the analysis of C3H8. The sensitivity of the
GC-FID are 66 times higher than GC-TCD method. In addition, the GC-FID exhibits
a wider linear range (0.161 -2.18%mol/mol) than the GC-TCD method (0.242 – 2.18
%mol/mol).

Keywords: propane; gas chromatography; thermal conductivity detector (TCD);

flame ionization detector (FID)

80
 087
086
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis of Asymmetric Curcumin analogues

from Cullilawan Oil using Conventional and
Microwave Method

Imanuel Berly Delvis Kapellea,c, Tun Tedja Irawadib, Meika

Syahbana Ruslia, Djumali Mangunwidjajaa, Zainal Alim
Mas’udb
a
Agricultural Industry Technology Department, Agriculture Technology Faculty, Bogor
Agricultural Institute, Indonesia
b
Chemistry Department, Mathematic and Natural Science Faculty, Bogor Agricultural Institute,
Indonesia
c
Chemistry Department, Mathematic and Natural Science Faculty, Pattimura University,
Indonesia

Abstract
Asymmetric curcumin analogues as potential anticancer compounds. The purpose
of this study was to synthesize product 5-benzo[1,3]dioxol-5-yl-1-phenyl-penta-2,4-
dien-1-one uses the method conventional and microwaves. Product 5-
benzo[1,3]dioxol-5-yl-1-phenyl-penta-2,4-dien-1-one can be synthesized from the
kulit lawang oil with several stages, among others; isolation safrole, isomerization,
oxidation and condensation reactions. Isolation of safrole from cullilawan oils
performed using NaOH solution and purified using distillation fractionation pressure
reduction produces 19.30% safrole are tested for purity by GCMS and for the
1
identification of the structure is done by using FTIR and H-NMR. The safrole
isomerization performed using KOH without solvent at a temperature of 120oC for 8
hours resulted isosafrole (91.53%) which consists of cis-isosafrol and trans-
isosafrol. Oxidation isosafrole performed using KMnO4 in acidic conditions using a
o
phase transfer catalyst tween 80 at a temperature of < 30 C and separation by
silica gel resulted in 65.63% piperonal were tested with the GCMS and identification
using FTIR and 1H-NMR. Products asymmetrical curcumin analogous made in the
condition alkaline by conventional methods for three hours produce 99.55%, a
method of microwaves in 140 watts for two minutes produce 82.82%.

Keywords: Cullilawan oils, synthesis, asymmetric curcumin analogues

81
 087
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Aluminum based composite by Novelty Process:

Repetitive Press Roll Bonding (RPRB)

Agus Pramonoa,b*, Lauri Kolloa, Renno Veinthala

a
Department of Materials Engineering, Tallinn University of Technology, Ehitajate tee 5, 19086
Tallinn, Estonia
b
Department Metallurgy, University of Sultan Ageng Tirtayasa, Jl.Jend Sudirman Km. 3
Cilegon Banten Indonesia 42435

Abstract
Composite is a combination of two or more materials to improve the mechanical
and physical properties. Strengthening and improvement of mechanical and
physical properties on element of metal matrix and ceramic reinforcing materials
depend on diffusion in the micro-structure. Diffusion of liquefaction on materials
usually requires coupling agent as a wetting agent. But in the high pressing will be
able to generate diffusion on aluminum as the matrix material and Al2O3 nanofiber
as reinforcement. This paper describes mechanism of evolution microstructure on
aluminum based composite material by new process of Severe plastic deformation
namely Repetitive Press Roll Bonding (RPRB). To ensure the evolution of the
microstructure, finite element analysis was used to help study the effect of
temperature on the microstructure changes that occur on aluminum based
composites.

Keywords: SPD, AA1070, Al2O3 nanofiber, Metal Matrix Composite and RPRB

82
 088
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Determination of crystallization and melting

behaviour of poly-lactic acid and polypropylene
blends as a food packaging materials by
Differential Scanning Calorimeter

Achmad Hanafi Setiawana

a
Polymer Chemistry Group, Research Center for Chemistry, Indonesian Institute of Science
Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314, Indonesia

Abstract
The food packaging materials commonly used is made from polymers synthetic
base on petroleum derivatives. However, the use of synthetic polymers has
negative impacts on the environment, because it is difficult to degrade naturally
either by biotic or abiotic process. This is a problem for the environment and
therefore it needs to do the assessment of the technology to reduce the degree of
difficulty on its degradation or to find a new material that can be degradable
naturally. One of the most important properties of food packaging materials is the
polymer crystallinity. This refers to the overall level of crystalline component in
relationship to its amorphous component. It is directly related to many of key
properties exhibited by a semi-crystalline polymer including brittleness, toughness,
stiffness or modulus, optical clarity, creep or cold flow, barrier resistance and long-
term stability. Thus, in this study, PP blends with the PLA in their hot solution for 4
hours, and investigates their crystallization and melting behavior using DSC at
o
cooling rate of 10 and 40 C/min. The results show that base on their thermograms,
with increasing the cooling rate will decreasing the crystallinity or increasing
amorphous area under the peaks.

Keywords: Food-packaging, materials, crystallization, PP/PLA, blend, DS

83
 089
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis of 1,4-butanediol monooleate and 1,4-

butanediol, 9-hydroxy-10-methoxy-monostearate
from Palm Oil as Modifier of Epoxy Resin for
Coating

Evi Triwulandari, Muhammad Ghozali, and Yenny Meliana

Research Center for Chemistry, Indonesian Institute of ScienceKawasan Puspiptek, Serpong
15314, Indonesia

Abstract
1,4-butanediol moonooleate (BMO) and 1,4-butanediol,9-hydroxy-10-methoxy-
monostearate (BHMMS) were synthesized. BMO was synthesized via Fisher
Esterification between oleic acid from palm oil and 1,4-butanediol by using sulfuric
acid as catalyst. Furthermore, BMO product was used as raw material for synthesis
of BHMMS. BHMMS was synthesis via continuous two-step reaction i.e.
epoxidation and hydroxylation. Characterizations of BMO and BHMMS products
were conducted by analysis of ester number, acid number, iodine number and
FTIR. In this research, obtained product of BMO and BHMMS were used as
modifier of epoxy by reacting with epoxy and tolonate to produce polyurethane
modified epoxy (PME) based on BMO (PMEBMO) and BHMMS (PMEBHMMS)
from palm oil. Characterization of PME was conducted by determine residual
isocyanate to calculate the isocyanate conversion and FTIR analysis. Curing
process of PME products to obtain film sheets were done by adding versamid 140
as hardener. Characterizations of film sheets were conducted by determine of
mechanical properties such as tensile strength and elongation at break. The result
showed that mechanical properties of PME were higher than the mechanical
properties of unmodified epoxy.

Keywords: epoxy, polyurethane, palm oil, BMO, BHMMS, modification

84
 090
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Bioethanol production from glucose by

thermophilic microbes from Ciater hot springs

Takiyah Salim*, Lia Ratnawati, Wawan Agustina, Sriharti

Center for Appropriate Technology Development, Indonesian Institute for Sciences ( Pusbang
TTG-LIPI ), Jl.K.S. Tubun No. 5, Subang 41213, Indonesia

Abstract
Bioethanol has been considered as one of the alternative energy resources for
fossil fuel substitute. Second generation of bioethanol production usually uses
lignocellulosic material as its raw material which conducted at high temperature
o
range (70-80 C). In this case the thermophilic microbe is needed for fermentation
process in order to minimize the use of energy. This paper will discuss the results of
the study on bioethanol production from glucose by using thermophilic microbes
isolated from local source namely from Ciater hot springs in Subang District,
Indonesia. In this study six thermophilic isolates (C1, C2, C3, C4, C5 and C6 ) were
tested their capability in producing ethanol in the fermentation medium containing
5% glucose substrate for 5 days incubation. To determine the activity of isolates in
ferment substrate is done by measuring the concentration of glucose and ethanol
produced using a spectrophotometer. Isolates tested ( C1,C2,C3,C4 and C5 ) could
reduce glucose concentration from 1.1up to 1.7 % in the fermentation medium. The
ethanol produced was tested qualitatively by reacting the samples with
K2Cr2O7inacidic conditions by observing its color change from yellow-orange to
green-blue. The presence of ethanol indicatedby the decrease of OD’s sample. This
study showed that all isolates have the ability to produce ethanol. However, there
are 2 isolates potentially produce ethanol that isolates C3 and C5 are characterized
by low absorbance after adding potassium dichromate (K2Cr2O7).

Keywords: thermophilic microbes, bioethanol, hot springs

85
 091
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Measurement and Prediction of Condensate Gas

Hydrates Formation Conditions – A Study Case

Mimoun Hadj2 ,Samer Said1, Otmanine Ghazi2 and Belloum

Mohamed1
1
Laboratory of Materials Science (LCMVAR), Dpt of Chemistry, Faculty of Science, University
Hadj Lakhdar of Batna,, Algeria
2
Laboratory of Hydrocarbons technology, Dpt of Chemistry, Faculty of Hydrocarbons &
Chemistry, University Mhamed Bouguerra of Boumerdes, Algeria.

Abstract
A new field of gas condensate recently discovered in south of Algeria caused a lot
of difficulties and even accidents during its operation due to unexpected hydrates
plug formation. To prevent such damage, we carried out an experimental study
using a visual high pressure PVT cell to investigate the thermodynamic conditions
of gas hydrates formation and stability, then we established hydrate formation
curves, identifying safety zones, allowing operators to work safely. The influence of
inhibitors on gas hydrate formation was also investigated. This study was gathered
with a numerical analysis using PVTsim commercial software to confirm our
experimental results.

Key words: hydrates, gas condensate, plug, PVT, safety

86
 092
International Symposium on Applied Chemistry 2015
(ISAC 2015)

87
 094
International Symposium on Applied Chemistry 2015
(ISAC 2015)

The Potential of Energy Efficiency on Recycle

Paper Bioprocess

Rina Masriani1,a *, Taufan Hidayat1,b, Nina Elyani1,b, Hendro

Risdianto1,b and Zeily Nurachman2,c
1
Center of pulp and paper, Jl. Raya Dayeuhkolot No. 132, Bandung 40258, Indonesia
2
Biochemistry Division, Faculty of Mathematics and Natural Sciences, Institute Technology
Bandung, Jl. Ganesha No 10, Bandung 40132, Indonesia

Abstract
The increasing use of recycled paper by the paper industry to support the Green
Industry in the Indonesian Pulp and Paper Industry. But technically, there are some
disadvantages of recycled fiber usage such as low drainage rate in which boosted
the high energy consumption on drying process. As already known, the drying
process consumes the highest energy in papermaking. To overcome this problem,
the research using the concentrate of endoglucanase Egl-II has done. The steps of
research included production of endoglucanase Egl-II; concentration by
ultrafiltration method, modification of recycled paper fibers using endoglucanase
Egl-II; characterization of paper sheet; and evaluation of energy consumption in
drying the sheet of paper. The results showed that endoglucanase Egl-II had
increase drainage rate of recycled fiber stock, which indicate by the freeness
number. The number had increased by 80 ml CSF (Canadian Standard Freeness)
from 190 ml CSF to 270 ml CSF, and fiber retention increased by 0,63% from
99,31% to 99,94 %. The dissolved cellobiose test results of recycled fiber stock by
the enzyme treatment at low dosage showed that no degradation of cellulose, no
change into soluble sugars. Characterization of sheet of paper that has been
modified with endoglucanase Egl-II based FTIR spectra showed no change in
functional group, SEM results showed on the fiber surface fibrils grow more, and the
results of the XRD analysis showed area reduction of amorphous regions is about
6%. The potential for energy efficiency is calculated through thermodynamic
approach is about 15 %.

Keywords: Area reduction of amorphous regions, endoglucanase Egl-II, energy efficiency,

fiber modification, recycled paper.

88
 095
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Comparison of Antiglycation and Antioxidant

Potentials and Total Phenolic Contents of
Decoctions from Antidiabetic Plants

P.R.D. Pereraa, S. Ekanayakeb*, K.K.D.S. Ranaweeraa

a
Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri
Jayewardenepura, Nugegoda, Sri Lanka
b
Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura,
Nugegoda, Sri Lanka

Abstract
Non enzymatic protein glycation and oxidative stress are the key molecular basis of
the macro and micro vascular complications observed in chronic diabetics.
Decoctions prepared with medicinal plants with antiglycation and antioxidant
potentials therefore have therapeutic potential in preventing diabetic complications.
Decoctions of five antidiabetic plants (parts) namely, Cassia auriculata flowers,
Osbeckia octandra leaves, Syzygium cumini bark, Phyllanthus emblica fruits and
Scoparia dulcis whole plant were analyzed for their DPPH and ABTS antioxidant
potentials, antiglycation potentials and total phenolic contents. Decoctions of S.
cumini, O. octandra and P. emblica had significantly high (p < 0.05) antiglycation
potential ranges of 16.8–35.2, 23.0–28.5 and 37.4–82.3 µg/ml with correspondingly
high antioxidant potentials and total phenolic contents of 851, 658 and 625 mg
GAE/g respectively.

Keywords: decoction; medicinal plants; antiglycation acticity; antioxidant potential

89
 096
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Polymerization of Blending Cholesteryl Acrylate

and Methyl Phenyl Benzoyl Acrylate

Afrizal1*, Muhammad Hikam2, Bambang Soegiyono2, Asep

Riswoko3
1
Faculty of Mathemathics and Natural Science,University Of State Jakarta, Jakarta, Indonesia
2
Department of Materials Science,University Of Indonesia, Depok
3
Center for Material Technology,BPPT, Jl. MH.Thamrin 8 Jakarta

ABSTRACT
Liquid crystal is a material which is between solid and liquid phase and commonly
called mesophase. Blends of liquid crystal are of great interest because of their
unique optical properties. Blending in this study using two monomers of liquid
crystal were cholesteryl acrylate and methyl phenyl benzoyl acrylate. The
polymerization process using uv curing techniques by irradiation UV ray and without
irradiation UV ray. Polymerization of blending liquid crystal acrylate using initiator 2-
hydroxy-2-methyl-1-phenylpropane. Based on peak at GPC curve of polymerization
by irradiation UV ray, type of that polymer is copolymer. Therefore the
polymerization without UV ray, type of that polymer is homopolymer. SEM images
of liquid crystal acrylate polymer showed lamella chain models that are
characteristic of a polymer chains. Type of polymer liquid crystal acrylate was the
type of Side Chain Liquid Crystalline Polymers (SCLCPs). Therefore acrylate
polymer liquid crystal in this research has semi-crystalline phase, which contained
crystalline phase and amorphous phase on the XRD pattern. The results of FT-IR
spectroscopic characterization of the two monomers showed a peak at the wave
-1 -1
number of 1600.43 cm and 1622.86 cm which indicates a double bond (C=C)
were obtained from acrylation. While the spectroscopy on the product blending the
wave number of the peak regions is reduced that shows that carbon double bonds
(C=C) in the acrylate group has polymerized. It also strengthened with a very sharp
-1
peak for CC functional groups on the wave number of 2855.15 cm . The results of
this study indicate that the liquid crystal polymer acrylic polymerization results with
radiation UV ray and without UV ray, respectively absorb light in the UV wavelength
region 363 nm and 351 nm.

Keywords : liquid crystal acrylate, blending, polymerization, UV Curing

90
 097
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Hydrogenation of Biomass-derived Furfural over

Highly Dispersed-Aluminium Hydroxide
Supported Ni-Sn(3.0) Alloy Catalysts

Rodiansonoa*, Maria Dewi Astuti, Uripto Trisno Santoso,

Shogo Shimazub
a
Department of Chemistry, Lambung Mangkurat University, Banjarbaru, South Kalimantan
70713
b
Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-
8522

Abstract
A novel method was applied for the preparation of highly dispersed nickel-tin (Ni-
Sn) alloy catalyst supported on aluminium hydroxide using a simple method at a
relatively low temperature. The addition of Sn (1.04 mmol) on the Raney nickel
supported on aluminium hydroxide (R-Ni/AlOH) to form Ni-Sn(3.0)/AlOH (3.0 is
Ni/Sn ratio) alloy catalyst remarkably enhanced the selectivity towards
hydrogenation of C=O rather than C=C in furfural giving high yield of furfuryl alcohol
almost exclusively. The Ni-Sn(3.0)/AlOH (3.0 is Ni/Sn ratio) alloy was also found to
be reusable without any significant loss of selectivity for at least six consecutive
runs.
Keywords: hydrogenation, biomass-derived furfural, furfuryl alcohol, Ni-Sn alloy
catalyst.

91
 098
International Symposium on Applied Chemistry 2015
(ISAC 2015)

The Initial Study of Polyaniline with Manganese

Oxides for Electrochemical Capacitors

Purnama Ningsiha*, Clovia Z. Holdsworthb and Scott W.

Donneb
a
Program Study of Chemistry Education, Department of Mathematics and Natural Science
Education, University of Tadulako, Palu 94221, Indonesia
b
Discpline of Chemistry, University of Newcastle, Callaghan, NSW 2308, Australia

Abstract
Conducting polymers have been found to be promising as electrode material in
energy storage devices such as electrochemical capacitors due to they have good
capacitance and relatively high charge/discharge rates, giving rise to higher energy
and power density than carbon-based electrochemical capacitors. Furthermore,
they have good chemical stability, high conductivity and are easy to manufacture.
The excellent performance of conducting polymers is due to their pseudocapacitive
charge storage mechanism. Polyaniline (PANI) is one of candidate of conducting
polymer has been used as material in this field; however, there are some
drawbacks to using conducting polymers as the electroactive material. Therefore,
conducting polymers require presence of another material which has good
mechanical and electrochemical behavior to address those problems. Manganese
oxides have good electrochemical performance by themselves, and are therefore
an excellent candidate for forming a composite with conducting polymers. Desirable
characteristics of manganese oxides include that they are abundant, low cost, and
have high specific capacitance. An initial study of the electrodepositionbehavior of
polyaniline-manganese oxides thin film was investigated using linear sweep
voltammetry (LSV), chronoamperometry (CA), and cyclic voltammetry (CV). Based
on the result of experiments, Mn-modified PANI thin films have been shown to be
promising materials for electrochemical capacitors electrodes.

Keywords: Polyaniline; Manganese oxides; Electrochemical capacitors; Thin films;

Linear sweep voltammetry; Chronoamperometry; Cyclic voltammetry

92
 099
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Isolation of thermophilic microbes resistant to

ethanol from Ciater hot springs, Subang-West
Java

Lia Ratnawati*, Takiyah Salim, Wawan Agustina, Sriharti

Center for Appropriate Technology Development, Indonesian Institute for Sciences ( Pusbang
TTG-LIPI ), Jl.K.S. Tubun No. 5, Subang 41213, Indonesia

Abstract
A study has been conducted to isolate thermophilic microbes from Ciater hot
springs. The aim of this research is to select thermophilic microbes that resistant to
ethanol. The methods of this research were : i. sampling, ii. enrichment, isolation
and screening, iii. identification, and iv. determination of minimum inhibition
concentration of ethanol. Enrichment, isolation and screening of thermophilic
microbes done by using Luria Broth (LB) medium contained 4% of ethanol.
Identification of isolates until genus level was done by comparing the results with
those of other studies. Determination of Minimum Inhibitory Concentration (MIC) of
ethanol was done by growing the isolates in the medium with different
concentrations of ethanol (0%, 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8%) and
incubated at 50oC, for 24 h. The result of this study was obtained 6 thermophilic
isolates which able to grow on a medium containing 4% ethanol ( C1, C2, C3, C4,
C5 and C6 ). From the results of MIC known that generally all isolates able to grow
well until the ethanol content of 6%, except for C6 isolate which only grow maximum
in 5% ethanol content. It was suspected that the bacteria isolated is a genus
Thermoanaerobacter.

Keywords: thermophilic , microorganism, bioethanol, hot-springs

93
 100
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Cracking Callophyllum innophyllum L.Oil to Bio-

gasoline by Micro-porous based Zeolite and Al2O3
Catalysts

Savitria*, R. Effendia, G. Primahanaa, S. Tursiloadia

aResearch Centre for Chemistry, Indonesian Institute of Sciences
Puspiptek Area Building 452, Serpong, South Tangerang, Banten, Indonesia (15314)

Abstract
Natural minerals such as zeolite are local natural resources in the various regions in
Indonesia which has application as a filler, bleaching agent, or dehydration agent. It
also has function as green catalysts material for biomass conversion into bio-fuel.
The trend movement of green and sustainable chemistry research that designing
environmentally friendly chemical processes from renewable raw materials to
produce innovative products derived biomass for bio-fuel. Callophyllum innophyllum
L.seeds can be used as raw material for bio-energy because of its high oil content.
The fatty acid and triglyceride compounds of these seeds can be cracked into bio-
gasoline, which does not contain oxygen in the hydrocarbon structure. This paper
focused on the preparation and formulation of the catalyst NiMo/Al2O3 and NiMo/H-
Zeolite which was used in hydro-cracking process of oil from Callophyllum
innophyllum L. seeds to produce bio-gasoline. The catalysts were characterized
using XRD, BET and FTIR-adsorbed pyridine method. The results of hydro-cracking
products mostly were paraffin (C10-C19) straight chain, with 59.5 % peak area
based on GC-MS analysist.

Keywords: Callophyllum innophyllum L.oil, hydro-cracking, bio-gasoline,

microporous catalysts, Zeolite, Al2O3

94
 101
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Development of Green Nickel-Based Zeolite

Catalysts for Citronella Oil Conversion to
Isopulegol

S. Tursiloadia, A. A.Litiazb, R. Pertiwia, I. B. Adilinaa and K.C.

Sembiringa*
a
Research Centre for Chemistry, Indonesia Institute of Sciences, Kawasan PUSPIPTEK
Serpong, Tangerang, 15314, Indonesia
b
Faculty of Science and Technology, State Islamic University Syarif Hidayatullah, Jln. Ir. H.
Juanda No. 95 Ciputat, Tangerang, Indonesia
Abstract
Derivatization of citronellol, citronellal and geraniol into fine chemicals such as
isopulegol, hydroxyl citronellal, menthol, geranial esters, esters citronellal and
others will escalate the added value of citronella oil. Some products derived from
essential oils are widely used for food additives, fuel additives, pharmaceuticals,
cosmetics and perfumes. The synthesis of isopulegol from citronellal was studied on
nickel-based zeolite catalysts. The citronella oil was obtained by vacuum fractional
distillation of lemongrass oil. The catalysts were prepared by supporting nickel on
natural zeolite from Bogor (ZAB) by impregnation method with nickel loading
amount variations. Catalytic cyclization reactions were carried out at 200 oC under
2 Mpa of H2 pressure for 3 h and were also varied the catalyst weight amount. The
products distribution was found to depend on the catalyst weight. The Ni/ZAB 20%
of metal loading catalyst (5 % weight) was highly active and selective towards
isopulegol (100% conversion and 57% selectivity).

Keywords: Citronella oil; citronella; H-zeolite; isopulegol.

95
 103
International Symposium on Applied Chemistry 2015
(ISAC 2015)

In vitro Anthelmintic Activity Evaluation of the

Aerial Part of Ruellia tuberosa Linn. Against
Eudrilus eugeniae

Kirstin Rhys S. Pueblosa*, Jeanne Phyre B. Lagarea, Radyn

Vanessa Phaz P. Tapalesa, and Mark Tristan J. Quimquea
a
Department of Chemistry, College of Science and Mathematics, MSU-Iligan Institute of
Technology,Andres Bonifacio Ave., Tibanga, Iligan City, 9200 Philippines

Abstract
The ever growing problems caused by perpetual helminth infections especially in third world
countries has paved way for scientists to continually search for potential sources of
alternatives. Ruellia tuberosa Linn., locally known as “cracker plant”, is a ubiquitous Philippine
medicinal that is traditionally used as remedy for a variety of diseases. In the light of plant-
based anthelmintic drug development, the present study was conducted to evaluate the
anthelmintic activities of the decoction (RTD), crude ethanol (RTE), chloroform-soluble (RTC)
and hexane-soluble (RTH) extracts and its fractions from the aerial part of R. tuberosa Linn.
against Eudrilus eugeniae (African night crawler earthworms). Anthelmintic assay was
employed at various concentrations in which the activity of the test samples was determined
by correlation with time. Results showed that in vitro anthelmintic assay of crude ethanol
extract relatively gave better results with respect to paralysis and death of earthworms
compared to its decoction counterpart. Meanwhile, both organic soluble extracts exhibited
notably better anthelmintic activities and were faster to bring about paralysis on the worms as
compared to the aqueous and alcoholic extracts. Furthermore, all fractions of the nonpolar and
mid-polar extracts showed significantly higher anthelmintic activities compared to its mother
extracts (RTH and RTC). Additionally, among all organic fractions tested, RTC4 showed fairly
high mortality rates incurred on the test organisms in which similar result was obtained in
comparison for its two parent crude extracts RTC and RTE. A concentration-dependent toxicity
relationship was also observed during the conduct of all bioassays. Subsequently, among all
extracts and fractions tested, RTC4 gave fastest mean time in paralyzing and killing the
earthworms (34.424.00 min and 36.114.32 min), thus, exhibited best anthelmintic activity that
is remarkably comparable to the positive control, mebendazole (54.582.17 min and
76.4912.60 min). Considering the results from the mid-polar extracts of the plant sample for
the given assay, R. tuberosa Linn. showed great potential for plant-derived anthelmintics.

Keywords: anthelmintic activity; helminthiasis; Ruellia tuberosa; African nightcrawler

96
 104
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Green Synthesis of Silver Nanoparticles using

Rizhome Extract of Galangal, Alpinia galanga

Alyza A. A. Azmia*, Norhidayah M. Ahyatb

a
School of Marine Science and Environment, Universiti Malaysia Terengganu, 21030,
Terengganu, Malaysia bSchool of Fundamental Science, Universiti Malaysia Terengganu,
21030, Terengganu, Malaysia

Abstract
The present study reports an eco-friendly, rapid and cost effective of the synthesis
of silver nanoparticles (Ag-Nps) using galangal, Alpinia galanga rhizome extract.
The antioxidant activity of the galangal rhizome, the family of Zingiberaceae was
detected through Fourier Transform Infra Red (FTIR) and total phenolic content
(TPC) assay. The FTIR showed the five vibrations of interest mode which were the
stretch of hydroxyl (O-H), alkyl, aromatics and carbonyl (C-O) functional groups at
-1 -1 -1 -1 -1
3400.8 cm , 2930.1 cm , 1638.6 cm , 1424.1 cm and 1053.5 cm and the TPC
of the galangal rhizome was determined. The silver nitrate (AgNO3) was used as
the precursor of the reaction and it was reduced to Ag-Nps using galangal rhizome
extract, a natural biological reducing agent via one-pot synthesis. The effects of
time of reaction and concentration of AgNO3 were observed. The Ag-Nps obtained
were confirmed by analyzing the excitation of surface plasmon resonance (SPR) via
UV-Vis spectrophotometer at around 431 to 438 nm. The spherical morphology of
Ag-Nps was confirmed by using SEM. The XRD spectrum corresponding to planes
(111), (200), (220) and (311) showed the crystalline nature of Ag-Nps formed was
face centre cubic (fcc). The resulted reported that the galangal, Alpinia galanga
rhizome was found to be effective biological reducing agent for the preparation of
Ag-Nps.

Keywords: Green synthesis ; Ag-Nps ; Alpinia galanga ; rhizome ; plant extract ;

TPC

97
 105
International Symposium on Applied Chemistry 2015
(ISAC 2015)

98
 106
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Structural and Morphological Analyses of Chitin

and its Complexupon Manganese Ion Adsorption

Meg Ina S. Acasa and Mark Tristan J. Quimquea,*

a
Department of Chemistry, College of Science and Mathematics, MSU-Iligan Institute of
Technology, Tibanga, Iligan City, 9200 Philippines

Abstract
The physical characterization of extracted chitin and its resulting complex upon
2+
adsorption with Mn ions was done through FTIRand SEM-EDX analyses. Chitin
was extracted from the exoskeletons of crabs using a conventional chemical
method.In the FT-IR analysis, the spectrum of the extracted chitin showed a
characteristic split in the amide 1 band which proved thesample to be in an alpha
configuration. The spectrum of the Mn(II)-chitin complex, compared to that of
the‘free’ chitin, showedan attenuation in the O-H and N-H stretching bands
which attributes to the probability of the attachment of the manganese ions inthese
functional groups. The EDX elemental analysis also supported the adsorption of
manganese onto the chitin surface of thecomplex. The SEM micrographs for
the‘free’ chitin appeared to have a rough and swollen morphology but after
adsorption ofmanganese ions, its surface became more compressed and
aggregated.

Keywords: chitin; Mn(II)-chitin complex; manganese ion adsorption; physical

characterization

99
 107
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Study on the Nitration Reaction of N,N-

diphenylamine Compounds and Its
Characterization as Organic Light Emitting Diode
(OLED) Material

Haryantoa,*, Deana Wahyuningruma, Anita Alnia

a
Organic Chemistry Research Division, Departtment of Chemistry, Faculty of Mathematics and
Natural Sciences, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung, 40132, Indonesia

Abstract
Organic Light Emitting Diode (OLED) is one of the developing technologies utilizing
organic compounds, especially aromatic compounds with conjugated double bonds.
The aims of this research is to study the transformation of N,N-diphenylamine (DPA)
to become potential compounds that can be utilized as precursor of OLED material.
In this research, DPA transformation have been done through nitration utilizing both
conventional and Microwave Assisted Organic Synthesis (MAOS) method. Various
o
reactions have been performed at 30 to 45 C, for 15 to 240 minutes, and the power
of the microwave reactor is 400 W. The nitration product of N,N-diphenylamine was
characterized with Fourier-Transform Infrared (FT-IR), UV-Vis, fluorescence, and
Nuclear Magnetic Resonance (NMR) spectroscopy. Based on the characterization
of the product, the nitration of N,N-diphenylamine has been successfully performed.
In addition, the maximum longwave UV-Vis spectrum absorption for the product in
acetone was 406 nm. For the emission spectrum, the product emitted light at 413
and 498 nm. The product was potential as OLED material because its ability to emit
light in the visible area.

Keywords: N,N-diphenylamine; MAOS; nitration reaction; OLED

100
 108
International Symposium on Applied Chemistry 2015
(ISAC 2015)

XPS Analysis and Antibacterial Assay of

Novobiocin Coating

Hardi Ysa* , Susan Sempleb , Hans J. Griesserc

a
University of Tadulako, Palu, Indonesia
b
School of Pharmacy and Medical Sciences-University of South Australia, Adelaide, Australia
c
Mawson Institute- University of South Australia, Adelaide, Australia

Abstract
Infection disease after biomedical implant surgery is often caused by unintentional
contamination of micro-organism. Novobiocinis one of the most attractive antibiotic
due to its high activity against gram positive bacteria and its relatively compatible to
fibroblast cell line. Based on the molecular structure of novobiocin, it may be
conjugated or coated onto material surfaces. Thecoating steps were plasma
polymerisation, polymer grafting, and novobiocin conjugation. XPS analysis showed
that the elementsand high resolution of C 1s confirmed that the surface chemical
had changed. Novobiocin, after conjugation, became moredominant in covering the
surface. Antibacterial Assay to Staphylococcus epidermidis by using dead and live
kit indicated thereduction of bacteria reached up to 97.27% and only 0.07% of live
bacteria left. This result led to a conclusion that immobilisednovobiocin may be
considered as novel antibacterial coating that can prevent nosocomial infection.

Keywords: novobiocin; plasma-polymerisation; antibacterial coatings; XPS

analysis

101
 109
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Functionalization of MCM-41 and SBA-15 Bottom

Ash with APTES

Nurul Barakah Ab Rahmana, Hamizah Md Rasida, Hashazirah

Mohammad Hassana, Mohammad Noor Jalila*
a
School of Chemistry and Environmental Studies Faculty of Applied Sciences,Universiti
Teknologi Mara, 40450 Shah Alam Selangor , Malaysia

Abstract
MCM-41 and SBA-15 were first synthesized by using TEOS and bottom ash supernatant as
silica source. Cetyltrimethylammonium bromide (CTAB) and Pluronic 123 (P123) were used
as template for MCM-41 and SBA-15, respectively. The mesoporous materials were modified
via post-grafting method using 3-aminopropyltriethoxysilane (APTES). Modified MCM-41 and
SBA-15 were characterized using Fourier Transform Infrared Spectroscopy (FTIR) to elucidate
the functional group and Field Emission Scanning Electron Micrograph (FESEM) to observe
their morphology. FTIR indicated the surfactant was removed after calcination and the APTES
was successfully grafted onto the pore of MCM-41 and SBA-15 with the presence of N-H
bend, -NH2 symmetric bend and CH2 group of the silylating agent. Modified mesoporous silica
will be characterized using X-Ray Diffraction (XRD) and Nitrogen adsorption to confirm their
hexagonal structure and pore size distribution retain even after modification.

Keywords: Mesoporous materials; MCM-41; SBA-15

102
 111
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Regenerating NADH enhances ethanol production

in aerobic cultureof recombinant Escherichia coli

Fithriania, Prayoga Suryadarmab,*, Djumali Mangunwidjajab

a
Department of Biotechnology, Postgraduate Bogor Agricultural University, Bogor 16680,
Indonesia
b
Department of Agroindustrial Technology, Faculty of Agricultural Engineering and
Technology, Bogor Agricultural University, Bogor 16602,
Indonesia

Abstract
The ethanol production in Escherichia coli harboring pyruvate decarboxylase (pdc)
and alcohol dehydrogenase (adhB) from Zymomonas mobilis was investigated by
regenerating intracellular NADH by introducing formate dehydrogenase (fdh) from
-1
Mycobacterium vaccae. Under aerobic conditions (KLa = 1.5 min ), the cells
-1
expressing pdc and adhB produced 0.4 g l ethanol when cultured for 18 h. This
-1
value was greater compared with the parent strain (0.2 g l ethanol). The
-1
production of ethanol was further enhanced at 1.79 g l in
-1
BW25113∆pta/pHfdh/pTadhB-pdc after 24 h cultivation with 4 g l formate
-1
supplementation(KLa = 6.1 min ). Formate was found not detectable at the end of
culture, indicating complete degradation this organic acid to regenerate NADH from
+
NAD . The culture strategy was effective to inactivate lactate dehydrogenase, which
is major competitor for ethanol production in utilizing NADH.

Keywords: aerobic culture; Escherichhia coli; NADH regeneration; ethanol production,

formate dehydrogenase

103
 112
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Multistages preparation for microfibrillated

celluloses based onArenga Pinnata “ijuk” fiber

Evana Yuanitaa,b,*, Juniko Nur Pratamab, Mochamad Chalidb

a
Center for Chemical and Packaging, Ministry of Industry, Jalan Balai Kimia No. 1 Pekayon
Pasar Rebo, Jakarta Timur 13069, Indonesia
b
Department of Metallurgy and Material Engineering, Faculty of Engineering, University of
Indonesia, Kampus Baru UI Depok 16436,
Indonesia

Abstract
Microfibrillated celluloses (MFC) extraction from “ijuk” fibers with chemically
multistages method has been investigated.Thefibers were prepared by
alkalinization with 10% sodium hydroxide (NaOH), oxidation with 3-6% sodium
hypochlorite (NaClO)and hydrolysis with 20 % sulphuric acid (H2SO4), respectively.
The alkalinization removes the fiber surface components such aslignin and wax so
the pore on the surface clearly appeared. Addition of NaClO leads an oxidative
fragmentation from remaininglignin and then the lignin dissolve out from the fiber.
Furthermore, hydrolysis fibrilates fiber into MFC and annihillates theamorphous
parts in MFC by keeping the crystalline parts. Fourier Transform Infrared (FT-IR)
confirmed the removal of somecellulosic components such as lignin, wax and
hemicelluloses, X-Ray Diffraction (XRD) reveals an increasing of crystalline partsin
the fiber. The micrograph by Field Emission Scanning Electron Microscopy
(FESEM) displayed dimension and surfacemorphology of MFC. The experimental
indicated that multistages preparation with 6% NaClO resulted better fibrillation
andcrystallinity than 3% NaClO. The microfibrillated celluloses resulted from this
multistages preparation, has some potentialapplications, such as filler and
nucleating agent in polymer, which are studied in the next study.
.
Keywords: “Ijuk” fiber, microfibrillated cellulose, alkali treatment,chlorine oxidation,
acid hydrolysis

104
 113
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Structural properties of Ni/γ−Al2O3 and Cu/γ−Al2O3

catalystand its application for hydrogenation of
furfurylidene acetone

Siti Mariyah Ulfa*, Inra Sari, Chairulita Putri Kusumaningsih,

M. Farid Rahman
a
Chemistry Department, Faculty of Science, Brawijaya University, Malang 65145, Indonesia

Abstract
Hydrogenation of furfurylidene acetone has been carried out using Ni/γ−Al2O3 and
Cu/γ− Al2O3 catalyst in the presence ofisopropanol in autoclave batch reactor. The
o
hydrogenation using Cu/γ− Al2O3 at 120 C for 6 h gives main formation of 1,5-bis-
o
(furan-2-yl)-pentan-3-one. Reaction at higher temperature at 140 C for 8 h using
Ni/γ− Al2O3 leads to 1,5-bis-(furan-2-yl)-penta-1-en-3-one. The different selectivity
of both catalysts is explained by physical properties including the surface area and
distribution of metal loading.

Keywords: hydrogenation; furfurylidene acetone; furfural; condensation;

temperature; catalyst

105
 114
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of CuO nanoparticle on mechanical and

thermal properties of palm oil based alkyd/epoxy
resin blend

Huei Ruey Onga,b, Md. Maksudur Rahman Khana,*, Ridzuan

Ramlib, Rosli Mohd Yunusa
a
Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang,
Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia
b
Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar baru bangi, 43000
Kajang, Selangor, Malaysia.

Abstract
In the present work, palm oil based alkyd resin was prepared with and without the
presence of CuO nanoparticles. The curing of the prepared alkyd resin with different
ratio of epoxy resin in the presence of poly(amido amine) as hardener was
investigated. The mechanical properties, such as tensile and flexural strength of the
cured blends were measured. The interaction between alkyd, epoxy and CuO
nanoparticle were investigated by using Fourier transform infrared spectroscopy
(FTIR). It was found that, crosslinking between alkyd and epoxy is possible through
the formation of interpenetrating polymer network (IPN). The thermal stability and
wettability of the blend was also investigated. It was found that, the CuO
incorporated nanocomposite presented greater thermal stability and hydrophobicity
than neat composite.

Keywords: Alkyd/Epoxy; CuO nanoparticles; FTIR; Thermal; Mechanical.

106
 115
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Enzymatic Conversion of Glycerol to Glyceric

Acid with Immobilised Laccase in Na-Alginate
Matrix

Chi Shein Hong, Sim Yee Chin, Chin Kui Cheng, Mahmud
Mohd Sabri,and Gek Kee Chua*
Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, 26300
Gambang, Pahang, Malaysia

Abstract
In this study, enzymatic oxidation of glycerol was performed for the production of
glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was
o
also verified. Glycerol was incubated at 25 C with 30 mM of 2,2,6,6-
tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in the presence
of immobilized laccase from Trametes versicolor. Glyceric acid was quantified using
HPLC, while the activity of enzyme was measured using ABTS assay. The yield of
laccase activity was 90%. On the other hand, glyceric acid obtained was around 7
wt% from the total product at 24h. In conclusion, enzymatic oxidation is able to
produce comparable amount of glyceric acid through a more environmental friendly
method.

Keywords: glyceric acid; immobilization; laccase; oxidation of glycerol; TEMPO

107
 116
International Symposium on Applied Chemistry 2015
(ISAC 2015)

MnO2/CNT as ORR Electrocatalyst in Air-Cathode

Microbial Fuel Cells

Chee Wai Woona, Huei Ruey Onga, Kwok Feng Chongb, Kar
Min Chana, Md. Maksudur Rahman Khana,*
a
Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang,
Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia
b
Faculty of Industrial Science and Technology, Universiti Malaysia Pahang, Lebuhraya Tun
Razak, 26300 Kuantan, Pahang, Malaysia.

Abstract
Air-cathode microbial fuel cell (MFC) is a potential electrochemical device for green
power generation simultaneously conducting wastewater treatment. In the present
work, the MnO2 catalyst has been prepared and modified by inducing carbon
nanotube (CNT) via sonochemical-coprecipitation method. The as-prepared catalyst
(MnO2/CNT) was characterized by x-ray powder diffraction patterns (XRD), field
emission scanning electron microscope (FESEM), energy-dispersive x-ray
spectroscopy (EDS), transmission electron microscopy (TEM) and cyclic
voltammetry (CV) to examine its morphological surface, crystal structure, elemental
analysis and oxygen reduction reaction (ORR) activity of the catalyst, respectively.
The CV results revealed that MnO2/CNT catalyzed ORR at potential of -0.45 V. The
effect of catalyst loading on the chemical oxygen demand (COD) removal efficiency
of palm oil mill effluent (POME) and MFC performance were studied. The maximum
power density and open circuit voltage (OCV) generated from with the as-prepared
MnO2/CNT were measured to be 215.57 mW/m3 and 582 V, respectively.

Keywords: MFC; MnO2/CNT; Oxygen reduction reaction; Polarization curves;

POME

108
 118
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Proficiency testing and laboratory performance in

the field ofpH measurement: An Indonesian
experiment

Nuryatini Hamima*, Sujarwoa, Ayu Hindayania, Christin

Elishiana, Oman Zuasa
Metrology in Chemistry Laboratory, Research Center for Chemistry, Indonesian Institute of
Sciences (LIPI), Kawasan PUSPIPTEK, Serpong,
Banten, 15314, Indonesia

Abstract
Proficiency testing (PT) for pH measurements for two consecutive years (2013 and
2014) in Indonesia have been conducted to discern the current condition of
participant laboratories regarding their capacity on the pH measurement and to
evaluate the effect on usage of the standard reference material. On 2013, two buffer
solutions (phthalate and phosphate) and their corresponding standard reference
solutions were distributed to the participant laboratory. While on 2014, the PT was
conducted for citrate buffer solution but no corresponding standard reference
solution was provided; thus the participant laboratory can used their own reference
standard. The PT for both phthalate and phosphate buffer in 2013 were completed
by involving 10 participant laboratories. In 2014, the PT was conducted with 90
participant laboratories. The PT results were evaluated based on ISO
13528:2005(E). From 10 participant laboratories on 2013, it was found that a 80%
of participant for phthalate buffer and a 70% of participant for phosphate buffer
attain a satisfactory category. For the PT of citrate buffer on 2014, it was found that
54 % of 90 participant have held a satisfactory category. Moreover, form the
evaluation regarding the effect of standard reference material usage; it was found
that a better result can be achieved by using a reference buffer solution from the
RCChem LIPI as the PT provider rather than by using their own standard reference.
Overall, it can be concluded that capability of the laboratory in Indonesia regarding
pH measurement is needed to be improved by which more attention is given in term
of the use and handling of standard buffer solution.

Keywords: Proficiency Testing, buffer solution, pH testing ,Laboratory

Performance.

109
 119
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Enzymatic Extract Fractionation of (Cymbopogon

winterianus Jowitt) Citronella through
Supercritical Carbon dioxide (SC-CO2)

Lloyd Arvin M. Malaluana, Roberto M. Malaluana

a
Department of Chemical Engineering & Technology, MSU-Iligan Institute of Technology, A.
Bonifacio Ave,Iligan City, 9200, Philippines
b
MSU-Naawan Foundation for Science and Technology Development, Inc.,Naawan,
Philippines

Abstract
Cymbopogon winterianus Jowitt or citronella is said to be an insect repellant and
usually planted in the yard in the Philippines. In this study enzymatic extracts of
citronella were fractionated using supercritical carbon dioxide extraction to have
different oil extracts in three different parameters, 10 megapascal (MPa), 20
megapascal (20MPa) and 30 megapascal (MPa) at constant temperature at 40°
Celsius. The highest oil yield is at 20MPa with an average of 10.21 for three trials of
extraction, followed by 10MPa and 30MPa with 4.89 and 2.15. Oil extract at 10MPa
was subjected to gas chromatography- mass spectrometry (GC-MS) and found four
components in which 2-methyl-5-(1-methylethyl)bicycle hexan 2-ol or trans
sabinene hydrate and 4-methanol-o-Menth-8-ene or elemol was the major
compounds. The pure crude enzymatic extract, 20MPa and 30MPa supercritical
carbon dioxide extracts were subjected to fatty acid profiling, twelve free fatty acid
were found at pure crude enzymatic extract, while nine free fatty acid were found at
20MPa and 30MPa supercritical carbon dioxide extracts. Lauric (C12) is
consistently highest in terms of weight by weight in three extracts that were
subjected to free fatty acid profiling. This study can be used as basis for agricultural
applications in food and pharmaceutical since there is no thorough study of the
fractionation of enzymatic extract of citronella.

Keywords: Supercritical carbon dioxide, citronella, gas chromatography- mass

spectroscopy, fatty acid profiling

110
 120
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Inhibition of Microbial Growth by Nano-chitosan

for Fresh Tilapia (Oreochromis sp) Preservation

Budhijanto Budhijantoa, Prihati Sih Nugrahenib, and Wiratni

Budhijantoa*
a
Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Jl.
Grafika 2 Yogyakarta 55281, Indonesia
b
Fishery Department, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Agro Yogyakarta
55281, Indonesia

Abstract
Nano-chitosan (chitosan nanoparticles) was tested as a novel preservation agent
for fresh tilapia fish (Oreochromis sp). The fish samples were immersed in 0.6%
nano-chitosan for 30 minutes and were kept in storage at room temperature, low
o o
temperature (10-15 C), and very low temperature (2-7 C). In room temperature,
nano-chitosan did not exhibit significant preservative effect to inhibit the growth of
microbes to be compared to sample without nano-chitosan treatment and kept in
room temperature (control). However at lowered temperature, a significant
difference was observed in term of decreasing fish deterioration rate for the nano-
chitosan treated samples to be compared to control. It is important to note that
o
lowering temperature to 2-7 C was not beneficial for preservation purpose because
nano-chitosan might agglomerate and lose its powerful inhibition to microbial
growth. The rate of deterioration was quantified using three parameter logistic
model for objective comparison among treatments, based on measured
physicochemical parameters (total volatile base and total plate count).

Keywords: nano-chitosan; preservation; tilapia; kinetic model; deterioration

111
 122
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Optimization of ketoprofen-loaded chitosan

nanoparticle ultrasonication process

Purwantiningsih Sugitaa, Laksmi Ambarsarib, and Lidiniyahc

a
Chemistry Department, Mathematics and Natural Science Faculty, Bogor Agricultural
University.
b
Biochemistry Department, Mathematics and Natural Science Faculty, Bogor Agricultural
University.
c
Chemistry Postgraduate Student, Bogor Agricultural University, Bogor, West Java, Indonesia

Abstract
Ketoprofen-loaded chitosan was made by modifying poloxamer 188 and also
varying time and amplitude of ultrasonication condition. Preliminary tests were
carried out by optimizing ultrasonication condition including amplitude and
sonication time. Nanoparticles characterization was carried out by using PSA, SEM,
FTIR, and XRD. Optimum condition of sonication process was obtained when
amplitude percentage and time used were 40% and 60 minutes, respectively. Three
test formulas give nanoparticle percentage more than 95% with an average particle
diameter below 400 nm.

Keywords: Chitosan, nanoparticle, poloxamer 188, ultrasonication

112
 123
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Potency of Rhizopora spp. Extracts as

Antioxidant and Inhibitor of Acetylcholinesterase

Wulan Tri Wahyunia,b, Latifah K. Darusmana,b, Nurzakiah Kahfi

Suryaa
a
Division of Analytical Chemistry, Department of Chemistry, Faculty of Mathematics and
Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16680,
Indonesia
b
Biopharmaca Research Center, Institute of Research and Community Empowerment, Bogor
Agricultural University, Kampus IPB Taman Kencana, Bogor 16151, Indonesia

Abstract
The potency of mangrove for traditional medicine has been widely investigated.
This study was conducted to investigate the potency of Rhizophora spp. extracts as
antioxidant and inhibitor of acetylcholinesterase (AChE). Leaves and fruits of
Rhizophora spp. were extracted using gradual polarity solvents, namely n-hexane,
ethyl acetate, and methanol. AChE inhibition assay was performed based on Ellman
method, meanwhile antioxidant was analyzed based on 2,2-diphenyl-pikrylhydrazyl
radical scavenging. Methanol extracts of Rhizophora spp. fruit consistently showed
inhibitory activity against AChE. Methanol extract of R. mucronata fruit showed
highest inhibitory activity with 50% inhibitory concentration (IC50) of 1 µg/mL, when
the IC50 of physostigmine was 0.06 µg/mL. Antioxidant assay against 2,2-diphenyl-
pikrylhydrazyl showed that the extract was a potential antioxidant with IC50 of 3.74
µg/mL whereas the IC50 of ascorbic acid was 1.31 µg/mL. The qualitative
phytochemical investigation showed that the extract contain tannin, saponin,
terpenoid, alkaloid, and flavonoid.

Keywords: acetylcholinesterase; antioxidant; inhibitor; mangrove; Rhizophora

113
 124
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Bioactivity of Polysaccharide from Gracilaria

verrucosa as α-Glucosidase Inhibitor

Vienna Saraswatya*, Tjandrawati Mozefa, Chandra Risdiana,

Abdullah Rasyidb
a
Research Centre for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu Sangkuriang
Bandung 40135, Indonesia
b
Research centre for Oceanography, Jl. Pasir Putih I, Ancol Utara, Jakarta, Indonesia

Abstract
Seaweeds are known to be important source of secondary metabolites for
pharmaceutical industry in drug development. Gracilaria genus are a potential
source for alternative natural medicines. One kind of Gracilaria species is Gracilaria
verrucosa. This species had been reported had potential as antioxidant,
antihypertension and many others. In this paper we reported for the first time the
bioacivity of methanol extract and crude polysaccharide from G. verrucosa as α-
glucosidase inhibitor. Polysaccharide of G. verrucosa was extracted from cell wall
by use of HCl pH 2 and washed in CaCl2 solution then identified by FTIR. The
results showed that crude polysaccharide B from G. verrucosa was found to be the
most active α-glucosidase inhibitor with IC50 of 3.51 ppm. Identification by FTIR
found that both crude polysaccharide A and B were similar to the spectra of agaro-
oligosaccharide.

Keywords: Gracilaria verrucosa ; α glucosidase ; polysaccharide

114
 125
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Synthesis of 2-Allylphenyl Cinnamate and Brine

Shrimp Lethality Test Activity Evaluation

Gian Primahanaa*, Teni Ernawatia, Puspa Dewi N. La, Indah

Dewijanti Dwiyatmia, Akhmad Darmawana, Muhammad Hanafia
a
Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan Puspiptek,
Serpong, Tangerang Selatan,Banten, 15314, Indonesia

Abstract
Synthesis of 2-allylphenyl cinnamate has been done via coupling reaction between
cinnamic acid and 2-allyl phenol catalyzed by DCC/DMAP. Their preliminary toxicity
activity evaluation was calculated using Brine Shrimp Lethality Test (BSLT) method.
Synthesis of the title compound was performed in 2 steps. Firstly, trans methyl
cinnamate isolated from Alpinia malaccensis was hydrolyzed to cinnamic acid using
2M ethanolic NaOH to gave trans cinnamic acid. Secondly coupling reaction of
cinnamic acid and 2-allyl phenol was conducted using DCC as activator and DMAP
1
as catalyst in cyclohexane. Structure elucidation was performed using LC-MS, H-
13
NMR and C-NMR. Spectroscopic data collected were confirmed that product
obtained from coupling reaction is 2-allylphenyl cinnamate. Brine shrimp lethality
test (BSLT ) activity evaluation of this compound showed that 2-allylphenyl
cinnamate with LC50 100.796 µg/mL has higher activity compared to their starting
material with LC50 120.470 µg/mL.

Keywords: 2-allylphenyl cinnamate; cinnamic acid; Brine Shrimp Lethality Test

115
 126
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Iron Carboyl Thiourea Hybrid Material Based on

Functionalised Silica from Rice Husk Ash for
Oxidation ofLimonene

Nadiah Ameram, Farook Adama*

School of Chemical Sciences,Universiti Sains Malaysia, 18000, Pulau Pinang,Malaysia.

Abstract
Iron carbonyl thiourea, RHACP1Fe was successfully immobilized onto inorganic
silica support from rice husk askvia chloropropyltriethoxysilane (CPTES) and the
resulting catalyst was labelled as RHACP1Fe. This mesoporousorganic-inorganic
2 -1
hybrid catalyst showed a specific surface area of 245.0 m g . The 29 Si MAS NMR
solid statespectrum showed the presence of Q4, Q3, T3 and T2 silicon centres in
RHACP1Fe. The oxidation of limonene withH2O2was studied using RHACP1Fe. A
moderate selectivity to the desired product (limonene peroxide) of 67 % and
amaximum limonene conversion of 60 % was achieved. RHACP1Fe could be
reused several times without losing itscatalytic activity.

Keywords: Iron Carbonyl thiourea; rice husk ash; chloropropyltriethoxysilane;

oxidation; limonene.

116
 132
International Symposium on Applied Chemistry 2015
(ISAC 2015)

The stability of Poly(ethersulfone) membranes

treated in hot waterand hypochlorite solution

Nasrul Arahmana*, Nursidika, Mukramaha, Sharvina

Maulidayantib, Ayu Octria Putric
a
Department of Chemical Engineering, Syiah Kuala University, Jl. Syeh A. Rauf No.7
Darussalam, Banda Aceh, Indonesia 23111.
b
Environmental Impact Management Agency of Sigli, Jl. Banda Aceh – Medan, Sigli, Indonesia
23111
c
Department of Chemical Engineering, Institute Technology of Bandung, Jl. Ganesha 10,
Bandung Indonesia, 40132

Abstract
In order to improve hydrophilic property and filtration performance, a membrane has
been modified by addition of polymericadditives in polymer solution. In the practical
application, it is necessary to control the stability of the modified membrane.
Thisstudy discusses filtration stability and hydrophilic property of four types of
polyethersulfone hollow fiber membrane preparedwith and without polymeric
additives. The effect of membrane treatment (soaked in hot water and hypochlorite
solution) on thefiltration performance and hydrophilic property was investigated.
Membrane consists of original PES membrane (M1), and threetypes of PES
modified membrane with three different additive (M2, M3, and M4). The stability
investigation was designed byusing single module of hollow fiber membrane
filtrated with deionized water during 10 days of filtration time. The
hydrophilicproperty change of membrane was analyzed by measuring water contact
angle. It was found that hydrophilic property andultrafiltration performance of
o
membrane M1 does not change after treated in water at temperature of 40 C and in
NaOClsolution. The treatment of membrane has also showed no impact on the
hydrofilic property and ultrafiltration performance ofmembrane M2, M3, and M4. A
significant change of filtration performance of membrane M2, M3, and M4 was
obtained after
membrane soaked in hypochlorite solution. Water permeability of those membrane
increased up to 50% after ten days offiltration.

Keywords: membrane stability, polyethersulfone, Ultrafiltration, water contact angle

117
 133
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Carbonylation of Methanol with CO in Presence

PotassiumMethoxide Catalyst

Hendriyanaa,b, Subagjob , Herri Susantob *

a
Chemical Engineering Department, University of Jenderal Achmad Yani, Jl. Terusan Jend
Sudirman, Cimahi, Indonesia
b
Chemical Engineering Department, Insitut Teknologi Bandung, Jl. Ganesa No. 10, Bandung,
Indonesia

Abstract
Catalysts prepared from potassium hydroxide and methanol were tested for
o 2
methanol carbonylation with CO in the range of 70-90 C and 20-25 kg/cm (g).
Methyl formate was produced selectively under the studied conditions. Lower
temperatures andhigher pressures enhanced the conversion of CO. Water in the
catalyst solution did not completion removed by molecular sievetype 3A, thus the
catalyst activity becomes decreased. The influence of inert gases in the gas mixture
will cause the partialpressure of CO decreased and also conversion of CO. The
reaction rate was first order with respect to the concentration of CO inthe gas
phase. The activation energy was found to be 14.95 kJ/mol and frequency collision
-1
factor is 0.59 min .

Keywords: potassium hydroxide; methanol; water; molecular sieve type 3A;

carbonylation.

118
 134
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Effect of Regeneration Temperature on Particle

Characteristics and Extent of Regeneration of
Saturated SO2-Adsorption of CuO/ᵒ-Al2O3 Adsorbent

David Bahrina, Subagjoa, Herri Susantoa*

a
Department of Chemical Engineering Institut Teknologi Bandung, Bandung 40132 Indonesia

Abstract
The effect of regeneration temperature on characteristics and extent of desorption
of saturated CuO/γ-Al2O3 havebeen studied. Adsorbent CuO/γ- Al2O3 (8%-Cu) was
prepared using the dry impregnation method withCu(NO3)2.3H2O solution on a
commercial γ- Al2O3. SO2 adsorption process of the adsorbent CuO/ᵒ- Al2O3
o
wereconducted on temperature of 400 C with a concentration of SO2 in a model gas
of 18500 ppmv and the initial weightof adsorbent of 1.0 gram. Regeneration of
saturated adsorbent were carried out under air flow at varioustemperatures of 500,
o
600 or 700 C. Both of adsorption and regeneration experiments were carried out
with aflowrate of model gas or air of 1.8 – 1.4 mL/sec for 60 minutes. Extent of
desorption of saturated adsorbent CuO/γ-Al2O3 were measured based on the
different weight of the adsorbent between the beginning and the end ofadsorption
o
and regeneration process. Regeneration temperatures of 500 and 600 C did not
o
significantly effect thespecific surface area of adsorbent. But that of 700 C affected
the specific surface area and average pore diameter ofthe adsorbent significantly.
The extent of desorption of the saturated adsorbent CuO/γ- Al2O3 at the
o o
regenerationtemperature of 600 C was higher than those of 500 and 700 C. Up to
four adsorption-desorption cycles, the extentof desorption were more less constant.
This experimental results reveal a promising method for the development offlue gas
desulfurization related the use of coal with a high sulfur content in power stations.

Keywords: characteristics; CuO/ᵒ-Al2O3; desorption capacity; pore properties

119
 135
International Symposium on Applied Chemistry 2015
(ISAC 2015)

Novel Synthesis, Antimicrobial, Antioxidant and

Antitumor Evaluation of Imidazolyl
Pyrazolopyridine and Imidazolyl
Pyrazoloquinoxaline Derivatives.

Mohamed A. El-Borai*, Mohamed E. Sadek, Mai M.El-Keiy

Department of Chemistry, Faculty of Science, Tanta University, 31527 Tanta, Egypt

Abstract
The cyanoacetylation of imidazole moiety gave 3-oxopropanonitrile derivatives Ia-c
,which were cyclized to give the corresponding 5-amino-pyrazoles IIa-c. The
reaction of compounds IIa-c with some active methylene reagents gave the fused
system imidazolyl pyrazolopyridines IIIa-c-VIIa-c. Also, the reaction of p-nitroso-
N,N-dialkyl aniline derivatives with IIa-c gave 3-imidazolyl pyrazolo[3,4-
b]quinoxaline-7-amine VIIIa-d. The synthesized compounds were prepared by
conventional heating and microwave irradiation techniques. The structure of
prepared compounds was confirmed by different spectroscopic tools. These
compounds were screened for their antibacterial activity against Gram-positive
bacteria, Gram-negative bacteria, and antifungal activity.Also, among the
synthesized compounds IIIa-VIIa tested for antitumor activity against liver and
kidney cell lines. The anti-oxidant activity of the prepared compounds was studied
using dipheny-picryl-hydrazyl radical (DPPH) assay.

Keywords: Imidazole, 5-aminopyrazole,pyrazolo[3,4-b]pyridine, pyrazolo-

quinoxaline, antibacterial, antioxidant and antitumor.

120
 LIST OF PARTICIPANTS

Name Institution Email

Achmad Hanafi Research Center for achmad.hanafi@gmail.c
Setiawan Chemistry om, achm045@lipi.go.id
University Of State
Afrizal afrizalkimia@gmail.com
Jakarta
Sembawa Research
Center - Indonesian
Afrizal Vachlepi a.vachlepi@gmail.com
Rubber Research
Institute
Department Metallurgy
and Material, University agus.pramono@ft-
Agus Pramono
Sultan Ageng Tirtayasa, untirta.ac.id
Indonesia
Research Center for
Agustine agustine_1408@yahoo.
Chemistry-Indonesian
Susilowati co.id
Istitute of Sciences
Akhmad Research Center for ahmaddarmawan@yaho
Darmawan Chemistry - LIPI o.com
School of Marine Science
and Environment, alyza.azzura@umt.edu.
Alyza A. A. Azmi
Universiti Malaysia my
Terengganu
Arief Ramadhan, Indonesian Rubber arifkaret@gmail.com;
M.Si Research Institute arif@puslitkaret.co.id
Universitas Katolik ariestya.arlene@yahoo.
Ariestya Arlene
Parahyangan, Indonesia com
Indonesia Islamic
Arif Hidayat arif.hidayat@uii.ac.id
University, Yogyakarta
Research Center for
aspiyanto_2010@yahoo
Aspiyanto Chemistry-Indonesian
.com
Istitute of Sciences
Faculty of Life Sciences
and Technology, Swiss
bea.anthika@hotmail.co
Bea Anthika German University, South
m
Tangerang 15339,
Indonesia
National Nuclear Energy budi23761@gmail.com,
Budi Setiawan
Agency, Indonesia bravo@batan.go.id
Institut Teknologi
Budiman Anwar bm.go.upi@gmail.com
Bandung
Bugi Ratno Indonesian Institute of budiarto_bugie@yahoo.
Budiarto sciences com
Candra Yulius Institut Teknologi tahya_candle@yahoo.co
Tahya Bandung m
Chelsea Chelsea_ichel@hotmail.
Swiss German University
Soedarma com
Universiti Malaysia
Chi Shein Hong sheinhong@gmail.com
Pahang
Daris daris.qodarisman@stud
Institut Teknologi
Qodarisman ents.itb.ac.id;
Bandung, Indonesia
Nasir rukman@chem.itb.ac.id
Desak Gede Sri Research Center for desakgedesa@gmail.co
Andayani Chemistry LIPI m
Desriyani LIPI participant
Center for Applied
Diah Dwiana
Nuclear Science and diahdwi@batan.go.id
Lestiani
Technology, Indonesia
Institut Teknologi dian.siti.shdh@gmail.co
Dian Siti S
Bandung m
Research Center for
Djaenudin Chemistry - LIPI, dian009@lipi.go.id
Indonesia
Pusat Penelitian Kimia -
Sri Hartati, Dr hartati12@yahoo.com
LIPI
Bandung Institute of
Eddy A. Basuki basuki@mining.itb.ac.id
Technology, Indonesia
ekam002@lipi.go.id;
Eka Mardika Research Centre for
eka_mardika@yahoo.co
Handayani Chemistry
m
Research Center for
ekatriwahyuni@gmail.co
Eka Triwahyuni Chemistry, Indonesian
m
Institute of Sciences
Faculty of Life Sciences
and Technology, Swiss
erico.himawan@student.
Erico Himawan German University, South
sgu.ac.id
Tangerang 15339,
Indonesia
Esti Nur Ubaeni UNJ Participant
Research Center for
Euis Hermiati Biomaterials, Indonesian euis.hermiati@lipi.go.id
Institute of Sciences
Department of Metallurgy
and Material Engineering, evana.yuanita@gmail.co
Evana Yuanita
Faculty of Engineering, m
University of Indonesia
Research Center for evi.triwulandari69@gmai
Evi Triwulandari
Chemistry l.com
 Research Center for evit002@lipi.go.id;
Evita Boes
Chemistry LIPI evitaboes@yahoo.com

feni.amriani@lipi.go.id;
Indonesian Institute of
Feni Amriani feni_chem1@yahoo.co
Sciences
m
fithria.03@gmail.com;
Bogor Agricultural
Fithriani p_suryadarma.ipb@ac.i
University
d
University of Gadjah
fitra.perdana@mail.ugm.
Fitra Perdana Mada, Jogjakarta,
ac.id
Indonesia
G. Herdiazto gha_93@rocketmail.co
Swiss German University
Adiyoga m
Gustan Pari R@D of Forest Products gustanp@yahoo.com
melanie.hakiki@gmail.c
Research Center for
Hakiki Melanie om;
Chemistry - LIPI
hakiki.melanie@lipi.go.id
Chemical Engineering
h_dahliana@yahoo.com
Department, Engineering
Halimatuddahlia ;
Faculty, Universitas
na Nasution halimatuddahliana@usu.
Sumatera Utara,
ac.id
Indonesia
Pusat Penelitian Kimia -
Hani Mulyani Participant
LIPI
Parahyangan Catholic
hans.kristianto@unpar.a
Hans Kristianto University, Bandung,
c.id; arenst@unpar.ac.id
Indonesia
University of Tadulako,
Hardi Y.S. hardiys@live.com
Palu, Indonesia
Indonesian Institute of
Harry Budiman Sciences-Research harr004@lipi.go.id
Center for Chemistry
Institut Teknologi haryanto2509@yahoo.c
Haryanto
Bandung om
Universiti Malaysia
Huei Ruey Ong roi_rui86@hotmail.com
Pahang
Center for Research and
Standardization of husniati@kemenperin.g
Husniati
Industry Bandar o.id
Lampung, Indonesia
mynameidris@gmail.co
Institut Teknologi m;
Idris
Bandung enny.rat@bdg.centrin.ne
t.id
 Center for Materials ika.maria87@gmail.com;
Ika Maria Ulfah
Technology - BPPT ika.maria@bppt.go.id
berly_mollucas@yahoo.
Imanuel Berly D Bogor Agriculture com,
Kapelle Institute, Indonesia berlykapelle1@gmail.co
m
Isna Participant
Central Mindanao
Ivy L. Librando ivy.librando@gmail.com
University
karimah@salam.uitm.ed
Karimah Kassim/
UiTM Shah Alam, u.my;
Siti Nurhazlin
Malaysia hazlinjaluddin@gmail.co
Jaluddin
m
Kiky Corneliasari
P2 Kimia LIPI kiky001@lipi.go.id
Sembiring
kirstinrhyspueblos@gma
Mindanao State
Kirstin Rhys S. il.com;
University-Iligan Institute
Pueblos kirstinrhys.pueblos@g.m
of Technology
suiit.edu.ph
Pusbang TTG - LIPI,
Lia Ratnawati lia.romeo@gmail.com
Indonesia
Institut Teknologi
Lin Marlina lin.basri@yahoo.com
Indonesia
Lina Nadzirah Universiti Teknologi
linadzirah@gmail.com
Binti Salahuddin MARA
Dept. of Chemical
Engineering and
Lloyd Arvin lloydarvinmalaluan@gm
Technology, MSU-Iligan
Malaluan ail.com
Institute of Technology,
Philippines
Madduma
Liyanage University of Colombo, Sri dulanthika88@gmail.co
Chinthani Lanka m
Dulanthika
Bandung Institute of
Mamay meyyouthful@gmail.com
Technology, Indonesia
Mark Tristan J. MSU - Iligan Institute of
mtjquimque@gmail.com
Quimque Technology
Maula Eka
BATAN maula@batan.go.id
Sriyani
Faculty of Pharmacy,
Melanny Ika sulistyowaty_melanny@
Universitas Airlangga,
Sulistyowaty yahoo.co.id
Surabaya, Indonesia
 Dpt of Chemistry, Faculty
of Science, University
Mimoun Hadj mimounhadj@yahoo.fr
Hadj Lakhdar of Batna,,
Algeria
Mohamad Raizul Universiti Teknologi
mdraizul@gmail.com
Bin Zinalibdin Malaysia
Mohamed El-
Tanta Univeristy, Egipt m_elborai@yahoo.com
Borai
MohmedAsif
mohmedasif.quraishi@s
IqbalHusain Shell India Market Pvt Ltd
hell.com
Quraishi
monafitria1985@yahoo.
Chemistry Departmen,
com;
Mona Fitria Institut Teknologi
mona.fitria@students.itb
Bandung, Indonesia
.ac.id
Institut Technology of monicaputri22@gmail.co
Monica Putri
Bandung m
Research Center for
Muhamad Nasir Chemistry - LIPI, mnasir71@yahoo.com
Indonesia
Biochemistry Research
Mukhammad asharychem@students.it
Group, Institut Teknologi
Asy'ari b.ac.id
Bandung, Indonesia
Research Center for
moer_yanto@yahoo.co
Muryanto Chemistry - LIPI,
m
Indonesia
Nabila Karina University of Sumatera nabilakarina97@gmail.c
Putri Utara, Indonesia om
Nadiah Ameram Malaysia nad87naj84@gmail.com
Norhanifah Binti
Malaysia Rubber Board norhanifah@lgm.gov.my
Mohd Yazid
Research Centre for
Nuryatini Chemistry -Indonesian atinur58@gmail.com
Institute of Sciences
Onen Amiruddin onen.attarwala@shell.co
Shell India Market Pvt Ltd
Attarwala m
University of Sri
rupika.perera@yahoo.co
P R D Perera Jayewardenepura, Sri
m
Lanka
Institute of Chemistry
Pankaj Sharma National Autonomus pankajsh@unam.mx
University of Mexico
Purnama.Ningsih@uon.
Purnama Ningsih University of Tadulako
edu.au
Purwantiningsih Department of Chemistry,
atiek_ps@yahoo.com
Sugita IPB, Bogor
 Mindanao State
Ramon M. mon.eduque@gmail.co
University - General
Eduque, Jr. m
Santos City, Phillippines
Institut Teknologi
Reza Aditama reza@chem.itb.ac.id
Bandung
Research Center for
Rina Andriyani Chemistry, Indonesian rina008@lipi.go.id
Institute of Sciences
Center of pulp and paper, rina.masriani@gmail.co
Rina Masriani
Ministry of Industry m
Research Center for
Rizna Triana
Chemistry-Indonesian riznatd@gmail.com
Dewi
Institute of Sciences
rodian114@gmail.com;
Lambung Mangkurat
Rodiansono rodiansono@unlam.ac.i
University
d
Institut Teknologi
Rukman Hertadi rukman@chem.itb.ac.id
Bandung
Research Center for
Sabar P
Chemistry - Indonesian saba004@lipi.go.id
Simanungkalit
Institute of Sciences
Dept. of Chemistry,
Sadiah
University Padjajaran, sadiah@unpad.ac.id
Djadjasupena
Indonesia
Prof. Sagarika
Sagarika University of Sri Ekanayake
Ekanayake Jayewardenepura <sagarikae@sjp.ac.lk> ;
sagarikae@hotmail.com
Santhy Wyantuti, Universitas Padjadjaran,
santhytfs@yahoo.co.id
Dr. Indonesia
Indonesian Institute of
Savitri savi002@lipi.go.id
Sciences
Setyo Faculty of Pharmacy -
tyas_ffua@yahoo.com
Prihatiningtyas Airlangga University
Jurusan Kimia FMIPA
Sherly Kasuma sherly_kasuma@yahoo.
Universitas Negeri
Warda Ningsih com
Padang
Siti Komariyah UNJ Participant
Siti Mariyah Ulfa Brawijaya University ulfa.ms@gmail.com
Research Center for
Sri Priatni sripriatni@yahoo.com
Chemistry LIPI

Mineral Processing
chnaziz@usm.my;
Sudibyo division, Indonesian
sudibyo@lipi.go.id
institute of science (LIPI)
 Research Center for
Teuku B.
Chemistry - LIPI, qaismajnun@gmail.com
Bardant
Indonesia
Tjandrawati Research Center for tjandrawm@yahoo.com;
Mozef Chemistry LIPI tjandrawm@gmail.com
ujipratomo31@gmail.co
m;
Uji Pratomo Unpad
uji_pratomo@yahoo.co
m
Research Centre for
Vera Barlianti vera001@lipi.go.id
Chemistry-LIPI
Willy Cahya Indonesian Institute of yoha004@lipi.go.id,
Nugraha Sciences will003@lipi.go.id
Chemical Engineering
Wiratni
Dept Universitas Gadjah wiratni@ugm.ac.id
Budhijanto
Mada
witt001@lipi.go.id;
Witta Kartika Research Center for
witta.kartika@yahoo.co
Restu Chemistry LIPI
m
Universiti Malaysia
Chee Wai Woon ahwei90@yahoo.com
Pahang
Research Center for
Yosi Aristiawan Chemistry - LIPI, yosi001@lipi.go.id
Indonesia
Research Centre For
myname_yulia@yahoo.c
Yulia Anita Chemistry,Indonesian
om
Institute Of Sciences
Yusran Abdillah Institut Teknologi yusran.am@gmail.com;
Muthahari Bandung rukman@chem.itb.ac.id
University of Malaya,
Zul Ilham ilham@um.edu.my
Kuala Lumpur, Malaysia
Davidhahrin@yahoo.co.i
David Bahrina ITB
d
hendriyana@lecture.unj
Hendriyana UNJANI, ITB
ani.ac.id
Center for Appropriate takiy_tsa@yahoo.com.a
Technology u,
Takiyah Salim
Development, Indonesian salimtakiyah@gmail.co
Institute for Sciences m
Vienna Research Center for
vsaraswaty@gmail.com
Saraswaty Chemistry LIPI
Research Center for wawan.kosasih@lipi.go.i
Wawan Kosasih
Chemistry LIPI d
Department of Chemistry,
Wulan Tri
Bogor Agricultural wulantriws@gmail.com
Wahyuni S
University
Diah Ratna Research Center for
diah009@lipi.go.id
Ningrum Chemistry LIPI
Research Center for gian.primahana@outloo
Gian Primahana
Chemistry LIPI k.co.id
Department of Chemical
Nasrul Arahman Engineering, Syiah Kuala nasrular@unsyiah.ac.id
University