7

SCIENCE
How to Use a Microscope
Quarter 2 - Week 2

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Science – Grade 7
Guided Learning Activity Kit
How to Use a Microscope
Quarter 2 - Week 2

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Guided Learning Activity Kit Development Team

Writer: Roan L. Rodriguez

Editor: Eleanor D. Almazan
Reviewer: Mae M. Alegado
Graphic Artist: Jennelyn B. Reyes

Management Team: Leonardo D. Zapanta EdD, CESO V

Michelle Ablian-Mejica EdD
Manolito B. Basilio EdD
Evelyn D. Tarrayo EdD
Garry M. Achacoso
Rachelle C. Diviva

Printed in the Philippines by Department of Education

Region III
Schools Division of Zambales
Zone 6, Iba, Zambales
Tel./Fax No. (047) 602 1391
E-mail Address: zambales@deped.gov.ph
Website: www.depedzambales.ph
 HOW TO USE A MICROSCOPE

Introduction

The unaided human eye is incapable to see the world in detail which is
needed to do many essential things in our modern lives. We use microscopes to
view minute organisms like bacteria, protozoa, algae, fungi, viruses, and other
microorganisms that are too small to be seen by the naked eye.
In the previous Guided Learning Activity Kit, we have discussed the parts
and functions of a microscope. We have also realized how microscopes opened a
whole new dimension in Science. It has paved way for many scientific discoveries
and breakthroughs.
In this Guided Learning Activity Kit, we will expand our knowledge on how
to use a microscope. We will explore wonders beyond what our naked eyes can see.

Learning Competency

Focus specimens using the compound microscope. (S7LT-IIb-2)

Objectives

At the end of this learning activity sheet, you are expected to:
1. enumerate the correct procedures on how to use a microscope;
2. observe specimens under a compound microscope; and
3. appreciate the role of a microscope in the field of Biology.

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 Review

On the previous Activity Sheet, we have familiarized ourselves with the

different parts and functions of a microscope. Let us have a quick review.

Label the parts:

Discussion

Well done! Now that you are already familiar with the parts and functions of
a microscope, let us talk about the correct procedures on how to use and take good
care of a microscope.
How to Use a Microscope
1. Get the microscope from its box or in the cabinet. Do this by grasping the
curved arm with one hand and supporting the base with the other hand.
2. Carry it to your table or working place. Remember to always use both hands
when carrying the microscope.
3. Put gently the microscope down on the laboratory table with its arm facing you.

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4. With the use of tissue paper or any clean and soft cotton rag, wipe the metal
parts of the microscope. You may also wipe the eyepiece and objectives
with a lens paper.
5. Look for the revolving nosepiece. Then, turn it clockwise or counterclockwise
until the LPO is back in its proper position.
6. Find the coarse adjustment. Slowly turn it upwards, then downwards.
7. Locate the eyepiece and look through it.
8. While looking through the eyepiece, adjust the position of the mirror so that it is
not glaring to the eyes. The bright circle of light is called the field of view of
the microscope. Practice viewing through the microscope using both eyes
open to avoid or reduce eyestrain.
9. Locate the diaphragm. While looking into the eyepiece, rotate the diaphragm to
the next opening.
10. Find the inclination joint. Grasp the arm and slowly pull it towards you. Sit
down and try looking through the eyepiece.

Observing Specimens
1. Put the slide with mounted small letter “e” on the stage holding it firmly with the
stage clips. Make sure that the letter is focused at the center of the hole on
the stage and under the LPO.
2. Watching from the side, carefully lower the body tube until the end of the LPO
almost touches the cover slip. (Note: Never let the objectives touch the slide
to avoid scratches in both lenses and mounted slide with the specimen.)
3. Look through the eyepiece. Slowly turn the coarse adjustment upwards to raise
the objective.
4. Look through the microscope again. Slowly move the slide to the right, then to
the left. What did you notice? When you move the slide to the right, the
image seems to move to the left. Likewise, when you move the slide up, the
image seems to move down.
5. Move the slide to the center. To shift to the HPO, raise the body tube first. Then,
using the fine adjustment slowly lower the objective till it almost touches the
cover slip. Looking through the eyepiece, turn the fine adjustment until you
see the clearest image.

How to take good care of a Microscope

1. Check the microscope before and after use.

2. Use a clean tissue paper or soft cotton cloth to clean the mechanical parts of the
microscope.

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3. Prevent liquids especially acids and alcohol from spilling on any part of the
microscope. Always use a cover slip when observing wet mounts.
4. Check for moisture. Wipe the lens with lens paper if there is any.
5. Avoid tilting the microscope while observing wet mounts. Water might flow into
the mechanical parts of the microscope causing them to rust.
6. Never store the microscope in a chemical laboratory or any place where there are
corrosive fumes.
Source: Science Grade 7 Learning Material

Activities
Guided Practice 1

SEQUENCING
Part 1: Directions: Arrange the following STEPS on how to use a compound
microscope. Write the correct sequence below. Write the NUMBERS only.

ANSWERS:
STEP 1: ______ STEP 2: ______ STEP 3: _______ STEP 4: _______
STEP 5: ______ STEP 6: ______ STEP 7: _______ STEP 8: _______
STEP 9: ______ STEP 10: _____

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Part 2: Directions: Arrange the following STEPS on how to observe specimens
under a compound microscope. Write the correct sequence below. Write the
NUMBERS only.

ANSWERS:

STEP 1: ______ STEP 2: ______ STEP 3: _______

STEP 4: _______ STEP 5: ______

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Guided Practice 2

LOOKING THROUGH!
Directions: Below are the images of the specimens focused under a
microscope. Describe the images shown.

1
2

4
5
Answers:
1. Letter “e”
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

2. Onion

___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

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3. Yeast
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

4. Pollen

___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

5. Blood

___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

Independent Practice

THINKING THROUGH!
Directions: Read and understand the article below. Then answer the guide
questions.
SMALL WONDER
An Essay by Roan L. Rodriguez
What if microscopes have never been invented? Have you ever thought about
what could our life be without a microscope?
The invention of the microscope is a milestone in the world of Science. It has
opened new dimensions in Science. Furthermore, it is considered as a
breakthrough in the field of Biology. Without a microscope, the knowledge
about cells would not have been possible. Using a microscope, scientists were able
to discover the existence of many microorganisms and were able to study life forms
that are not visible. As a result, cures to diseases have been discovered. In 1905,
using an ordinary microscope, Robert Koch discovered the bacillus that causes
tuberculosis. It is considered to be one of the deadliest diseases at that time. The
said discovery had saved thousands of lives. Today, laboratories in hospitals use
microscopes to detect microorganisms that cause infections. Hence, physicians can
prescribe the proper antibiotic. They are likewise used in diagnosing cancer and
other diseases.

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 Scientists, doctors, and researchers never stopped looking for a cure to
diseases. To date, doctors from all over the world are still yearning to find a cure
for Covid-19. Microscope is one of the instruments that is widely used in search of
its cure.
As a member of the biotic components, let us be grateful for this invention.
Care to our loving mother earth would be better through this “small wonder” in
technology - the MICROSCOPE!
GUIDE QUESTIONS:

1. What are the benefits from the invention of the microscope?

________________________________________________________________________
________________________________________________________________________
________________________________________________________________________

2. Who discovered the bacillus that caused tuberculosis? What is the effect of this
discovery?

________________________________________________________________________
________________________________________________________________________
________________________________________________________________________

3. Why is the article entitled “Small Wonder”?

________________________________________________________________________
________________________________________________________________________
________________________________________________________________________

Assessment

FILL IN THE BLANKS

Directions: Using the WORD BANK below, fill in the blanks with the correct
answer.

field of view slide microscope

tuberculosis both hands center
chemical laboratory fine adjustment tilting
cells mechanical diaphragm
HPO rust rectangular
inverted right up

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1. The bright circle of light is called the _______________ of the microscope.
2 - 4. When carrying the microscope always remember to use our ____________
avoid ___________the microscope while observing wet mounts. Water might
flow into the mechanical parts of the microscope causing them to
__________.
5. Specimens observed using a microscope is in ___________ position.
6-7. Put the ____________ on the stage. Make sure that the letter is at the
_____________ of the hole on the stage and under the LPO.
8-10. If the slide is moved left while looking through the microscope, it will appear
to move _________, and if moved towards you, it will seem to move _______.
11. While looking into the eyepiece, rotate the ____________to the next opening.
12-13. To shift to the _____________, raise the body tube first. Then, using the
_____________ slowly lower the objective till it almost touches the cover slip.
14-15 When observed under a microscope, onions have _____________, interlocking
____________.

Reflection

Part 1. FILL IN THE BLANKS

Directions: Using the WORD BANK below, fill in the blanks with the correct
answer. (Note: Answers may be used more than once)

inverted specimen

microscope procedures

After all the exciting activities in this Guided Learning Activity Kit, I now
understand the correct (1)_______________ on how to use the (2)________________. I
also learned the proper ways on how to observe (3) ___________ under a
(4)_____________. Lastly, I have realized that specimens observed using a
microscope is in (5) ___________ position.

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Part 2.

Directions: Write a short paragraph. Choose one (1) from the titles listed
below.

1. Uses of Microscope

2. How to Use a Microscope

3. Microscope: A Breakthrough in the Field of Science

Answer:

___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

Be guided by the following scoring rubrics.

Criteria 5 3 1

Content and Demonstrate an Demonstrate a Show some

Organization excellent good misunderstanding
understanding of understanding of of the topic. There
the topic. the topic. Some is no clear
Information is information is introduction or
relevant and somewhat conclusion.
presented relevant and
logically. presented
logically.

Grammar, All sentences are Most of the Fairly structured

Structure, and well-structured, sentences are sentences and
unique writing well-structured, writing style, with
Style
style, and no good writing style, grammatical errors
grammatical but with and misspelled
errors or grammatical words.
misspelled words. errors and
misspelled words.

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 References

2015. https://www.researchgate.net/figure/CELL-MORPHOLOGY-OF-
YEAST-UNDER-COMPOUND-MICROSCOPE_fig1_280318748.

2020. 3.Bp.Blogspot.Com. https://3.bp.blogspot.com/-

z0AW8xKAU7g/VLbRX90nnBI/AAAAAAAAANI/Hq1_iOtHzbo/s1600/
sunflowerpollen.jpg.

"Amazon.Com". 2020. Amazon.Com. https://www.amazon.com/Peripheral-

Blood-Film-smear-Microscope/dp/B005XCUIB6.

Ferido, Marlene, and Jacqueline Rose Gutierrez. 2012. Science Grade 7

Learning Material. Department of Education.

Ferriols-Pavico, Josefina, Genevieve Darwin-Faraon, and Anna Cherylle

Morales-Ramos. 2017. Exploring Life Through Science. 2nd ed.
Quezon City: Phoenix Publishing House.

"Microscopy". 2005. Csun.Edu. http://www.csun.edu/scied/7-

microscopy/microscopy-techniques/microscopy_index.html.

Rodriguez, Roan L. 2020. Unpublished text.

"Using The Compound Microscope In Class". 2020. Microscopy.

https://serenalegeremicroscopy.weebly.com/using-the-compound-
microscope-in-class.html.

Vectors, Royalty, and Cartoon Vectors. 2020. "Young Boy Cartoon Using
Microscope Vector Image On Vectorstock". Vectorstock.
https://www.vectorstock.com/royalty-free-vector/young-boy-cartoon-
using-microscope-vector-1791887.

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ASSESSMENT GUIDED PRACTICE 1
1. Field Of View Part 1 Part 2
2. Both Hands 1. Step 1- 3 2. Step 1 - 5
3. Tilting Step 2- 5 Step 2 - 4
4. Rust Step 3 – 9 Step 3 - 3
5. Inverted Step 4 – 7 Step 4 - 2
6. Slide Step 5 – 10 Step 5 - 1
7. Center Step 6 – 1
8. Microscope Step 7 – 8
9. Right Step 8 – 2
10. Up Step 9 – 4
11. Diaphragm Step 10 – 6
12. HPO
13. Fine Adjustment GUIDED PRACTICE 2
14. Rectangular Answers may vary
15. Cells Must include description of the given
specimen under a microscope.
REFLECTION
INDEPENDENT PRACTICE
Part 1: Answers may vary
Sample answers:
1. Procedures
1. It is considered as a breakthrough
2. Microscope in the field of Science especially in
3. Specimens Biology.
4. Microscope 2. Robert Koch. It has saved many
5. Inverted lives even until our time.
3. As a student, I may use my
Part 2: knowledge in using a microscope in
observing life forms that are not
Answers May Vary
visible to the naked eye.
Key to Corrections
 Acknowledgement

The Schools Division of Zambales would like to express its heartfelt

gratitude to the following, who in one way or the other, have contributed to the
successful preparation, development, quality assurance, printing, and distribution
of the Quarter 2 Guided Learning Activity Kits (GLAKs) in all learning areas across
grade levels as a response to providing the learners with developmentally-
appropriate, contextualized and simplified learning resources with most essential
learning competencies (MELCs)-based activities anchored on the principles of
guided learning and explicit instruction:
First, the Learning Resources (LR) Development Team composed of the
writers and graphic artists for devoting much of their time and exhausting their
best efforts to produce these indispensable learning kits used for the
implementation of learning delivery modalities.
Second, the content editors, language reviewers, and layout evaluators
making up the Division Quality Assurance Team (DQAT) for having carefully
evaluated all GLAKs to ensure quality and compliance to DepEd standards;

Third, the Provincial Government of Zambales, for unceasingly extending its

financial assistance to augment the funds for the printing of these learning
resources for use by learners and parents at home;
Fourth, the teacher-advisers and subject teachers, in close coordination
with the school heads, for their weekly distribution and retrieval of the GLAKs and
for their frequent monitoring of the learners’ progress through various means; and
Finally, the parents and other home learning facilitators for giving the
learners the needed guidance and support for them to possibly accomplish the
tasks and for gradually helping them become independent learners.
To deliver learning continuity in this challenging circumstance would not be
possible without your collective effort and strong commitment to serving our
Zambaleño learners.

Again, our sincerest thanks!

The Management Team

 For inquiries or feedback, please write or call:

Schools Division of Zambales

Zone 6, Iba, Zambales
Tel./Fax No. (047) 602 1391
E-mail Address: zambales@deped.gov.ph
Website: www.depedzambales.ph