Bial's Orcinol Test Result Substance Tested Color Reaction Description
Bial's Orcinol Test Result Substance Tested Color Reaction Description
https://microbenotes.com/bials-test/
http://dept.harpercollege.edu/chemistry/chm/100/dgodambe/thedisk/carbo/bial/bials.htm
QUESTION
5. Differentiate Benedict’s Test and Fehling’s Test. State the advantages of using the former over the latter. Whay
there is a need to freshly prepare the Fehling’s reagent? Why is the Fehling’s test not used in analyzing carbohydrates in
clinical samples?
The Benedict's test identifies reducing sugars (monosaccharide's and some disaccharides), which have free ketone or
aldehyde functional groups. In Benedict's test the reagent is very stable, single solute is prepared and storage is
convenient, it is both a qualitative and quantitative test, auto reduction doesn’t take place and it less sensitive. Fehling's
test is used to differentiate between reducing and non-reducing sugars. Can also use to distinguish ketone functional group
carbohydrates and water-soluble carbohydrates. In Fehling's test the reagent is unstable, it has to prepared in two parts, it
is only a qualitative test and it is more sensitive. Fehling’s solution is always prepared fresh in the laboratory. It is made
initially as two separate solutions, known as Fehling’s A and B. Fehling’s A is a blue aqueous solution of copper (II)
sulphate pentahydrate crystals, while Fehling’s B is a clear solution of aqueous potassium sodium tartrate and a strong
alkali. The Fehling’s test not used in analyzing carbohydrates in clinical samples, because Fehling’s reagents is unstable,
is only qualitative test, auto reduction of cupric hydroxide occur resulting in false positive test.
https://microbenotes.com/fehlings-test/#:~:text=Fehling's%20test%20is%20a%20chemical,carbohydrates%20and
%20water%2Dsoluble%20carbohydrates.
https://microbiologyinfo.com/benedicts-test-principle-composition-preparation-procedure-and-result-interpretation/
https://www.onlinebiologynotes.com/fehlings-test-objective-principle-reagents-procedure-and-result/
https://www.scribd.com/doc/209336404/Carbohydrates