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Bial's Orcinol Test Result Substance Tested Color Reaction Description

The document describes the results of Bial's Orcinol test on various substances. Xylose showed a strong positive result, indicated by a bluish color. The control (water), glucose, lactose, and starch all showed negative results, indicated by a yellow or yellow-brown color. Bial's test detects pentoses and pentosans by hydrolyzing them into pentoses, which are then dehydrated to form furfural and condense with orcinol to produce a blue-green precipitate, indicating a positive result. A bluish color with xylose confirmed it contains pentoses.

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0% found this document useful (0 votes)
5K views2 pages

Bial's Orcinol Test Result Substance Tested Color Reaction Description

The document describes the results of Bial's Orcinol test on various substances. Xylose showed a strong positive result, indicated by a bluish color. The control (water), glucose, lactose, and starch all showed negative results, indicated by a yellow or yellow-brown color. Bial's test detects pentoses and pentosans by hydrolyzing them into pentoses, which are then dehydrated to form furfural and condense with orcinol to produce a blue-green precipitate, indicating a positive result. A bluish color with xylose confirmed it contains pentoses.

Uploaded by

Xyrelle Navarro
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Bial’s Orcinol Test Result

Substance Tested Color reaction Description


Control (Water) The color is yellow. The color indicates
a negative result for pentoses, so it is not
a pentose.

Glucose The color is yellow-brown that indicates


a negative result for pentoses, so it is not
a pentose.

Xylose The color is bluish, and this color


indicate as a strong pentose positive.

Lactose The color is yellow-brown that indicates


a negative result for pentoses, so it is not
a pentose.

Starch The color is yellow-brown that indicates


a negative result for pentoses, so it is not
a pentose.
DISCUSSION
Bial’s test is a chemical test performed to detect the presence of pentoses and pentosans (derivatives of pentoses). A
derivation of this test termed the Bial’s Orchintest is performed to detect the presence of RNA in solutions. This test is
based on the principle that under hydrolysis pentosans are hydrolyzed into pentoses. Further, pentoses are dehydrated to
yield furfural, which in turn condense with orcinol to form a blue-green precipitate. Bial’s test is performed to detect the
presence of pentoses and pentosans (derivatives of pentoses). A derivation of this test termed the Bial’s Orchintest is
performed to detect the presence of RNA in solutions. Bial's reagent contains concentrated HCl as a dehydrating acid,
orcinol and ferric chloride as condensation reagent. A positive test (xylose) is indicated by the formation of a bluish
product. All other colors (yellow and yellow-brown) indicate a negative result (control (water), glucose, xylose, lactose,
and starch) for pentoses.

https://microbenotes.com/bials-test/
http://dept.harpercollege.edu/chemistry/chm/100/dgodambe/thedisk/carbo/bial/bials.htm

QUESTION
5. Differentiate Benedict’s Test and Fehling’s Test. State the advantages of using the former over the latter. Whay
there is a need to freshly prepare the Fehling’s reagent? Why is the Fehling’s test not used in analyzing carbohydrates in
clinical samples?

The Benedict's test identifies reducing sugars (monosaccharide's and some disaccharides), which have free ketone or
aldehyde functional groups. In Benedict's test the reagent is very stable, single solute is prepared and storage is
convenient, it is both a qualitative and quantitative test, auto reduction doesn’t take place and it less sensitive. Fehling's
test is used to differentiate between reducing and non-reducing sugars. Can also use to distinguish ketone functional group
carbohydrates and water-soluble carbohydrates. In Fehling's test the reagent is unstable, it has to prepared in two parts, it
is only a qualitative test and it is more sensitive. Fehling’s solution is always prepared fresh in the laboratory. It is made
initially as two separate solutions, known as Fehling’s A and B. Fehling’s A is a blue aqueous solution of copper (II)
sulphate pentahydrate crystals, while Fehling’s B is a clear solution of aqueous potassium sodium tartrate and a strong
alkali. The Fehling’s test not used in analyzing carbohydrates in clinical samples, because Fehling’s reagents is unstable,
is only qualitative test, auto reduction of cupric hydroxide occur resulting in false positive test.

https://microbenotes.com/fehlings-test/#:~:text=Fehling's%20test%20is%20a%20chemical,carbohydrates%20and
%20water%2Dsoluble%20carbohydrates.
https://microbiologyinfo.com/benedicts-test-principle-composition-preparation-procedure-and-result-interpretation/
https://www.onlinebiologynotes.com/fehlings-test-objective-principle-reagents-procedure-and-result/
https://www.scribd.com/doc/209336404/Carbohydrates

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