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Qualitative Test For Proteins

This document describes various color tests that can be used to identify different amino acids in proteins. It lists the name of 8 common color tests used in protein analysis - Biuret test, Ninhydrin test, Xanthoproteic test, Millon's test, Pauly's test, Sakaguchi test, Sulfur test, and Hopkins-Cole test. For each test, it provides the observed color change, corresponding amino acid detected, and reagents used. In conclusion, it summarizes that the tests can specifically identify amino acid groups or individual amino acids like tyrosine, tryptophan, arginine, and cysteine/methionine based on color reactions.

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Jeandra Villanueva
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141 views2 pages

Qualitative Test For Proteins

This document describes various color tests that can be used to identify different amino acids in proteins. It lists the name of 8 common color tests used in protein analysis - Biuret test, Ninhydrin test, Xanthoproteic test, Millon's test, Pauly's test, Sakaguchi test, Sulfur test, and Hopkins-Cole test. For each test, it provides the observed color change, corresponding amino acid detected, and reagents used. In conclusion, it summarizes that the tests can specifically identify amino acid groups or individual amino acids like tyrosine, tryptophan, arginine, and cysteine/methionine based on color reactions.

Uploaded by

Jeandra Villanueva
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Villanueva, Jhulia Alessandra Color Reactions of Protein

BSN 1 – 4
Lab Apparatus:
Beaker
Test Tubes
Test Tube Rack
Test Tube Holder
Dropper
Alcohol Lamp
Reagent Bottle

Biuret Test Purple color (+) Purple Peptide bond


Ninhydrin Test Purple color (+) Purple NH2 group

Xanthoproteic Test Yellow to orange (+) Aromatic amino


a. With HNO3 Yellow color acids
Orange color Yellow to orange (Tyrosine,
b. With NaOH (sodium Tryptophan, and
hydroxide) Phenylalanine)

White precipitate
which changes to
Millons Test Red Tyrosine
brick red on boiling
(+)
Pauly’s Test Cherry red color (+) Cherry red Tyrosine or Histidine
Sakaguchi Test Red color (+) Red to orange Arginine
Black precipitate Sulfur-containing amino
Sulfur Test formed (+) Black acids (Cysteine and
methionine)
Purple ring formed
at the junction
Hopkins – Cole Test Purple Tryptophan
between two layers
(+)

Reagent Used:
Biuret Test: NaOH and CuSO4 (sodium hydroxide and copper sulfate)
Ninhydrin Test: C9H6O4 (ninhydrin solution)
Xanthoproteic Test: NaOH and HNO3 (sodium hydroxide and concentrated nitric acid)
Millons Test: Hg(NO3)2 and HNO3 (mercuric nitrate and nitric acid)
Pauly’s Test: C6H7NO3S, NaNO2, and Na2CO3 (sulfanilic acid, sodium nitrite, and sodium carbonate)
Sakaguchi Test: NaOH, C10H8O, and Br2 (sodium hydroxide, alpha naphthol and bromine water)
Sulfur Test: NaOH and Pb(C2H3O2)2 (sodium hydroxide and lead acetate)
Hopkins – Cole Test: CH2O, HgSO4, and H2SO4 (formaldehyde, mercuric sulfate, and concentrated
sulfuric acid)

CONCLUSION:
Ninhydrin test was specific for the NH2 group present in a protein.
Xanthoproteic was a test to identify aromatic amino acids like Tyrosine, Tryptophan, and
Phenylalanine.
Millons test was to identify if tyrosine was present in a protein.
Pauly’s test was to detect aromatic amino acid tyrosine or histidine.
Sakaguchi test is a test to detect the presence of arginine.
Sulfur test was to identify the presence of sulfur-containing amino acids.
Hopkins-Cole test was to determine if a protein has tryptophan in it.

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