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Potassium: 20 Test 1x60ml

This document provides instructions for determining the level of potassium in serum using a colorimetric method. Potassium levels are important to monitor as elevated or decreased levels can be associated with various health conditions. The method uses sodium tetraphenylboron to produce turbidity proportional to potassium concentration, which is measured via spectrophotometry. Reagents, storage, sample requirements, assay procedure, calculations, reference values and sources are described.

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Dinesh Sreedharan
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48 views3 pages

Potassium: 20 Test 1x60ml

This document provides instructions for determining the level of potassium in serum using a colorimetric method. Potassium levels are important to monitor as elevated or decreased levels can be associated with various health conditions. The method uses sodium tetraphenylboron to produce turbidity proportional to potassium concentration, which is measured via spectrophotometry. Reagents, storage, sample requirements, assay procedure, calculations, reference values and sources are described.

Uploaded by

Dinesh Sreedharan
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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POTASSIUM

(COLORIMETRIC METHOD) (Turbidimetric)


For the determination of potassium in serum
(For In vitro Diagnostic Use Only)

SUMMARY:

Potassium is the principle cation of the intra-cellular fluid. It is also an important constituent of the extra-cellular
fluid due to its influence on muscle activity. Its intra-cellular function parallels that of its extra-cellular function,
namely influencing acid-base balance and osmotic pressure, including water retention. Elevated potassium levels
(hyperkaliemia) are often associated with renal failure, dehydration shock or adrenal insufficiency. Decreased
potassium levels (hypokaliemia) are associated with malnutrition, negative nitrogen balance, gastrointestinal
fluid losses and hyperactivity of the adrenal cortex.

PRINCIPLE:

The amount of potassium is determined by using sodium tetraphenylboron in a specifically prepared mixture to
produce a colloidal suspension. The turbidity of which is proportional to potassium concentration in the range of
2-7 mEq/l.

Tetraphenyl Boron + K+ ---------► White Turbidity

CONTENT:

PACK SIZE POTASSIUM REAGENT(A1) STANDARD (S)


20 Test 1x60ml 5ml

STORAGE AND STABILITY:

Store all reagents at 2-8°C. The reagents are stable until the expiration date indicated on the package label.

REAGENT PREPARATION:

Reagents are ready to use.

SAMPLE:

Serum is recommended. Potassium in serum is stable for at least 2 weeks 2-8°C. Specimen should be free from
hemolysis and blood specimens should also be separated from red cells shortly after collection. Plasma from
anticoagulants not containing potassium is also suitable.
GENERAL SYSTEM PARAMETERS:

Reaction Mode End point Sample Volume 50 µl


Wavelength 620 nm Reagent volume 1000 µl
Blank Reagent blank Standard 5 mmol/L
Incubation 5 min at R.T Reaction Slope Increasing
Delay Time 5 Sec Linearity 8 mmol/L
Read Time 5 Sec Units mmol/L

ASSAY PROCEDURE:

Pipette into a clean dry test tubes labeled as Standard (S) and Test (T) :
Addition Sequence B(µl) S(µl) T(µl)
Potassium 1000 1000 1000
Reagent(A1)
Standard(S) - 50 -

Sample - - 50

Mix and allow it to stand at room temperature for 5 min. read & record the absorbance at 620 nm of all tubes.

CALCULATION :
Abs.of Test
Potassium (mmol/L) = ----------------------------- × 5
Abs. of standard

NORMAL REFERENCES VALUES:

Serum : 3.4 - 5.3 mmol/L


It is strongly recommended that each laboratory establish its own normal range. Respecting its patient population.

LINEARITY : This method is linear up to 8 mmol/L

NOTES:
Turbid or icteric samples produced falsely elevated results. Bilirubin above 40 mg/dl, Urea nitrogen above 80
mg/dl and hemolyzed sera will produce elevated results. Sera containing high levels of ammonia should be
avoided.

REFERENCE:

1. Henry RF et al. Clinical Chemistry Principles and Technics, 2nd Ed., Harper and Rox, Hagerstown, M.D. (1974).
2. Tietz NW. Fundamentals of Clinical Chemistry WB, Saunders Co., Phila, PA, P. 874.
3. Terri AE and Sesin PG, Am. J. Clin. Path., 29:86 (1958).

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