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Triglycerides

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Dinesh Sreedharan
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66 views1 page

Triglycerides

Uploaded by

Dinesh Sreedharan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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TRIGLYCERIDES ASSAY PROCEDURE:

(GPO-PAP Method)
Wavelength/ Filter : 546 nm
For the determination of Triglycerides in serum and plasma
(For In vitro Diagnostic use only) Temperature : R.T/37°C
Light Path : 1 cm
SUMMARY:
Triglycerides are the form of fatty acid esters. They are produced in the liver by binding Pipette into clean dry test tube labeled as Blank (B), Standard (S) and Test (T)
glycerol and other fatty acids. They are transported by VLDL and LDL and act as a storage Addition Sequence B(µl) S(µl) T(µl)
source for energy.
Triglycerides Reagent(A1) 1000 1000 1000
PRINCIPLE:
Standard(S) - 10 -
Lipoprotein Lipase
Triglycerides Glycerol + Free fatty acids Sample - - 10
Glycerol Kinase Mix well and incubate at 37°C for 10 min or at R.T. for 15 min. Measure the
Glycerol + ATP Glycerol 3 Phosphate + ADP absorbance of the standard (Absorbance of Standard) and test Sample (Absorbance
Glycerol 3 PO of Test) against reagent blank at 546 nm within 60 min.
Glycerol 3 Phosphate + O2 Dihydroxyacetone Phosphate + H2O2
Peroxidase CALCULATION:
H2O2 + 4 Aminoantipyrine + Phenol Purple Quinoneimine dye + H2O Absorbance of Test
The intensity of the color formed is directly proportional to the amount of Triglycerides Triglycerides in mg/dl = --------------------------------------- × 200
present in the sample. Absorbance of Standard
CONTENTS:
NORMAL REFERENCE VALUES:
PACK SIZE TGL REAGENT (A1) STANDARD (S) Serum and Plasma (Suspicious): 150 mg/dl and above
1x50ml 1x50ml 2ml (Elevated) : 200 mg/dl and above
2x50ml 2x50ml 2ml It is recommended that each laboratory establish its own normal range representing its
4x25ml 4x25ml 2ml patient population.

STORAGE / STABILITY: LINEARITY:


Triglycerides reagent is stable at 2 - 8°C till the expiry mentioned on the label. This procedure is linear upto 1000 mg/dl. If values exceed this limit , dilute the sample
Triglycerides Standard is stable at 2-8°C till the expiry mentioned on the label. with normal saline(NaCl 0.9%) and repeat assay. Calculate the value using the proper
REAGENT PREPARATION: dilution factor.
Reagents are ready to use. Protect from bright light.
SAMPLE: NOTE:
Serum or Plasma. Triglycerides is reported to be stable in the sample for 5 days at 2-8°C. Fasting Samples of 12 to 14 hours are preferred. Fatty meals and alcohol may cause
elevated results. Patients should not drink alcohol for 24 hours before the test.
GENERAL SYSTEM PARAMETER:
Reaction Mode End Point Sample Volume 10 µl
REFERENCES:
Wavelength 546 nm Reagent volume 1000µl 1. Trinder.P., (1969) Ann. Clin. Biochem . 6:24
Blank Reagent Standard 200 mg/dl 2. Bucolo.G, David.H (1973) Clin. Chem 19:476
Incubation 10 min 37°C/15 min R.T Reaction Slope Increasing 3. Fossati.P, Prencipe.L (1982) Clin. Chem. 28:277
Delay Time 5 Sec Linearity 1000 mg/dl
Read Time 5 Sec Units mg/dl

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