Cambridge International Examinations

Cambridge International Advanced Subsidiary and Advanced Level

BIOLOGY 9700/34
Paper 3 Advanced Practical Skills 2 May/June 2018
CONFIDENTIAL INSTRUCTIONS
Great care should be taken to ensure that any confidential information given, including the identity
of material on microscope slides where appropriate, does not reach the candidates either directly or
* 8 1 9 4 3 9 0 6 4 4 *

indirectly.

If you have any queries regarding these Confidential Instructions, please contact Cambridge stating the
Centre number, the nature of the query and the syllabus number quoted above.

email info@cie.org.uk
phone +44 1223 553554
fax +44 1223 553558

This document consists of 10 printed pages and 2 blank pages.

DC (ST/SG) 137913/3
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Instructions for preparing apparatus

These instructions give details of the apparatus required by each candidate for each experiment in
this paper. A summary of the questions that will be presented to the candidates is included, where
appropriate, to allow the biology teacher to test the apparatus appropriately.
No access to the Question Paper is permitted in advance of the examination.
Candidates must be provided with a microscope with:
• eyepiece lens, ×10 (equal to 16 mm or 23″)
• low-power objective lens, ×10 (equal to 16 mm or 23″)
• high-power objective lens, ×40 (equal to 4 mm or 16″)
• eyepiece graticule fitted within the eyepiece and visible in focus at the same time as the
specimen.
To avoid confusion, only the lenses specified above should be fitted in the microscopes to be used in
the examination. Any lenses which are not ×10 or ×40 should be removed or replaced.
Each candidate must have uninterrupted use of the microscope for at least one hour.
Supervisors are advised to remind candidates that all substances in the examination should be treated
with caution. Pipette fillers and suitable eye protection should be used where necessary.
In accordance with the COSHH (Control of Substances Hazardous to Health) Regulations, operative in
the UK, a hazard appraisal of the examination has been carried out.
The following codes are used where relevant.
C corrosive MH moderate hazard
HH health hazard T acutely toxic
F flammable O oxidising
N hazardous to the aquatic environment

internal diameter

When small test-tubes are provided, it is expected

that these are approximately 150 mm in height.
height
If other dimensions of apparatus are required, these
will be specified.

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Confidential Instructions

For both questions

Each candidate will require:

• ruler, marked in mm
• clean and dry apparatus, e.g. glassware and syringes (without needles)
• solutions supplied in suitable beakers or containers for removal of the solutions using syringes
• fresh solutions, materials and rinsing water where appropriate.

More of the solutions should be available if requested by candidates.

If a candidate breaks any of the apparatus or loses any of the materials supplied, the matter should be
rectified and a note made in the Supervisor’s Report.

Solutions should be disposed of in accordance with local safety regulations.

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Question 1

Each candidate will require:

materials and apparatus for each candidate quantity 3

[F][MH][HH] 15% ethanol in a beaker or container, labelled E, provided at at least
room temperature (see Preparation of materials) 50 cm3
[F][MH][HH] 10% ethanol in a beaker or container, labelled U, provided at at least
room temperature (see Preparation of materials) 20 cm3
20% glucose solution in a beaker or container, labelled G, provided at room at least
temperature (see Preparation of materials) 100 cm3
Distilled water, in a beaker or container, labelled W, provided at room at least
temperature 100 cm3
Beaker, labelled Y, holding 6 test-tubes with 1 g of dried yeast in each 1 beaker
test-tube. The test-tubes should be large, capacity 30 cm3 to 50 cm3 + 6 test-tubes
Universal Indicator paper, 5 cm lengths in a shallow container, labelled P, at least
provided with a pH colour chart 3 lengths
10 cm3 syringes, with the means to wash them out 3
Beakers or containers, capacity 75 cm3 to 100 cm3 5
Test-tube rack(s), to hold 6 large test-tubes or test-tube rack, to hold 1
5 test-tubes plus a container to hold 1 large test-tube
Beaker, capacity approximately 400 cm3, with water between 50 °C and 1
60 °C, labelled Hot water
The Supervisor may use a thermostatically controlled water-bath to provide
additional hot water if requested by candidates
Beaker, capacity approximately 400 cm3, with cold water below 30 °C, 1
labelled Cold water
Beaker or container, capacity 400 cm3, labelled Water-bath, to act as a 1
water-bath and be large enough to hold 6 large test-tubes
Thermometer, –10 °C to 110 °C 1
Container, capacity approximately 200 cm3, with tap water labelled 1
For washing
Container, capacity approximately 400 cm3, labelled For waste 1
White tile 1
Paper towels 8
Glass marker pen (permanent) 1
Glass rod 1
Scissors 1
Stop-clock or timer showing seconds 1
Suitable eye protection 1

It is advisable to wear suitable eye protection when handling chemicals.

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Preparation of materials

Solution G may be prepared the day before the examination and kept in a covered container in a
refrigerator.
All the materials should be at room temperature before the start of the examination.

[F][MH][HH] (i) E, 15% ethanol

This is prepared by putting 15 cm3 of 99% ethanol into 50 cm3 distilled water. Make
up to 100 cm3 with distilled water.

[F][MH][HH] (ii) U, 10% ethanol

This is prepared by putting 10 cm3 of 99% ethanol into 50 cm3 distilled water. Make
up to 100 cm3 with distilled water.

There should be no naked flames in the room where the examination is carried
out.

(iii) G, 20% glucose solution

This is prepared by sprinkling 20 g of glucose into 50 cm3 of distilled water, stirring

well. Make up to 100 cm3 with distilled water.

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Question 2

Each candidate will require:

materials and apparatus for each candidate quantity 3

7% yeast in 2% sucrose solution in a beaker or container, labelled Y, at least
provided at room temperature (see Preparation of materials) 20 cm3
[HH][MH][N] Methylene blue solution in a beaker or container, labelled M, at least
provided at room temperature and kept out of direct sunlight. 10 cm3
This must be prepared as in Preparation of materials.
Iodine solution in a beaker or container, labelled I, provided at room at least
temperature and kept out of direct sunlight. 10 cm3
This must be prepared as in Preparation of materials.
Microscope with an eyepiece graticule fitted into the eyepiece lens 1
(as described on page 2)
• the microscope must be set up on low power
• the slide must not be left on the stage of the microscope
Microscope slides and coverslips 3
Seeker or mounted needle 1
Pipette, plastic or glass with teat with the means to wash them out 2

Container, capacity approximately 100 cm3, with tap water, labelled 1

For washing
Container, capacity approximately 100 cm3, labelled For waste 1
Paper towels 8
Glass marker pen (permanent) 1

Preparation of materials

(i) Y, 7% yeast suspension in 2% sucrose solution

2% sucrose solution
This is prepared by sprinkling 2 g of sucrose, a little at a time, onto the surface of 80 cm3 of
distilled water, stirring continuously as you sprinkle. Make up to 100 cm3 with distilled water.

7% yeast suspension in 2% sucrose solution

This should be prepared 30 minutes before the candidates start Question 2.
In a large container add 7 g of dried yeast to 40 cm3 of warm 2% sucrose solution.
Stir and make up to 100 cm3 with warm sucrose solution. This should be kept for approximately
30 minutes at a temperature between 35 °C and 40 °C.

To help put the 20 cm3 of Y into the beaker for each candidate, it is suggested that the yeast
suspension is poured into a second beaker, leaving the froth behind.

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[HH][MH][N] (ii) M, 0.1% methylene blue solution

Put 0.1 g of methylene blue [HH][MH][N] into 80 cm3 of distilled water in a beaker.
Make up to 100 cm3 with distilled water.

If any methylene blue comes into contact with your skin wash off immediately with
water.

(iii) I, 0.01 mol dm–3 iodine solution

This is prepared by firstly making a 0.1 mol dm–3 iodine solution.

Put 8 g of potassium iodide into a beaker or container. Moisten the potassium iodide
with a few drops of distilled water. Add 2.5 g of iodine to the potassium iodide and
stir well. Make up to 100 cm3 adding small volumes of distilled water and stirring
well. Continue to stir until the iodine has dissolved.

Then put 10 cm3 of this iodine solution (0.1 mol dm–3) into a beaker or container
and make up to 100 cm3 with distilled water. This makes the 0.01 mol dm–3 required
for candidates.

This solution must be made up just before the start of the examination and kept out
of direct sunlight.

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SUPERVISOR’S REPORT

The Supervisor’s Report is essential in order to allow the Examiners to assess all candidates as fairly
as possible and should always be completed by every Centre.

During the examination, the Supervisor or other competent Biologist (not the Invigilator), should follow
the steps in Question 1, in order to obtain results for 1(a)(ii) and 1(a)(iii).

The Supervisor should use the same solutions as those provided to the candidates and work out of
the sight of the candidates.

These results should be written in the Supervisor’s Report, not on a spare question paper.

SEATING PLAN

Provide a seating plan of work benches, on separate paper, giving details of the places occupied by
the candidates for each question using each candidate’s number.

The Supervisor’s Report and the candidates’ seating plan should be enclosed with each packet of
scripts.

MATERIALS TO BE SUPPLIED BY CAMBRIDGE

There are no materials to be supplied by Cambridge.

RETURN OF EXAMINATION MATERIALS TO CAMBRIDGE

There are no materials to return to Cambridge.

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BLANK PAGE

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BLANK PAGE

Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.

To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge International
Examinations Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download at www.cie.org.uk after
the live examination series.

Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local
Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge.

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9700/34
This form should be completed and sent with the scripts

SUPERVISOR’S REPORT

May/June 2018

The Supervisor or Teacher responsible for the subject should provide the following information.

1 Was any difficulty experienced in providing the necessary materials? If so, give brief details.

2 Give details of any difficulties experienced by particular candidates, giving names and candidate
numbers. Reference should be made to:

(a) difficulties arising from faulty specimens or microscopes;

(b) accidents to apparatus or materials;
(c) assistance provided in case of colour-blindness;
(d) any other information that is likely to assist the Examiner, especially if this cannot be
discovered from the scripts.

All other cases of individual hardship, e.g. illness or disability, should be reported directly to Cambridge
on the normal ‘Special Consideration Form’ as detailed in the Cambridge Handbook.

3 During the examination the Supervisor or other competent biologist (not the invigilator) should
follow the steps in Question 1 in order to obtain results for 1(a)(ii) and 1(a)(iii).The Supervisor
should use the same solutions as those provided to the candidates, and work out of the sight of
the candidates.
These results should be written on page 12, which should be enclosed with the candidates’
scripts. If the scripts are in several packets, please ensure that a copy of the Supervisor’s Report
is enclosed with each packet of scripts.

4. Enclose a seating plan of work benches with the scripts, giving details of the candidate numbers
for the places occupied by the candidates for each question.

Declaration (to be signed by the Supervisor)

The preparation of this practical examination has been carried out so as to maintain the security of the
examination.

Signed .....................................................................................................................................................

Name (in block capitals) ..........................................................................................................................

Centre number (of enclosed scripts) .......................................................................................................

Centre name ............................................................................................................................................

If scripts are despatched in more than one packet, it is essential that each packet includes a copy of
the:
• relevant Supervisor’s Report
• appropriate seating plan(s).
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Temperature of examination room ......................... °C

Results for Question 1(a)(ii) and 1(a)(iii)

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