CYTOSPIN 3

74010101
Issue No 02

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CELL PREPARATION SYSTEM

SlWNDONI(>I

Shandon Scientific Limited is an IS0 9001 Approved Company.

 SERVICE MANUAL
information contained in this document is intended for use by

I
qualified personnel only. The calibration, scheduled servicing and
the removal and replacement of parts should only be undertaken
by personnel who have been formally trained in the appropriate
servicing techniques and procedures.
I
 LIFE SCIENCES INTERNATIONAL (EUROPE) LTD

SERVICE IidANUAL RECEIPT SKEET

When you receive this Cytospin 3 Service Manual, please complete the
following:

Company/Agent Name: . ... .. . ..... .. . . . ... .... ... .. .. ... .. .. . ............ ..... ... ... .. .. .. .........

Address: .....................................................................................................

........................................................................ ........................................... ..

.................................................................................................................... .

Signature: .... .... . .... . .. ... .. .. .. . .. ..... .. . . . .. ... .. ....L.. ... ..... .. ... ...... ...... ..... ... .. ....... .....

Please return this completed Receipt Sheet to the Service Department at

Life Sciences international (Europe) Ltd by fax (0928 565845) or by letter
post to:

Life Sciences international (Europe) Ltd

International Service Department
Chadwick Road, Astmoor
Runcorn, Cheshire, WA7 IPR
England

These sheets will be used to complete our records and ensure you
receive any amendments as and when they become available.

International Service Manager

 SELANDONCYTOSPIN
3

MICROPROCESSOR
CONTROLLED
CENTRIFUGE

SERVICE:HANUAL

This manual has been designed as an aid to efficient servicing and

fault diagnosis on the Cytospin 3 centrifuge machine.

In order to get the best results from the Cytospin 3 please consult
the operators manual.
The service manual for the Cytospin 3 bench top centrifuge is designed
to provide the locally based engineer with all the information he
needs to install and maintain the Cytospin in good running order. It
is essential that the maintenance routines are followed so that the
operator gets the most out of the Cytospin.

In the event of a problem arising, the section on Fault Diagnosis

should be referred to. This section contains information designed to
help the service engineer locate and repair simple faults. For a more
detailed description of the operation of the Cytospin 3, the
Principles of Operation section should be referred to.

If you think you have identified and resolved the cause of a fault,
remember to carry out any calibration of the part you have repaired or
replaced, if necessary. Before handing over the Cytospin to the
operator, make sure you have checked it out thoroughly using the
serviceability test.

Finally, the manual provides you with a comprehensive list of any

parts you may need to order.

Whilst every care has been taken to ensure that the information
contained in this manual is correct, no liability can be accepted by
Shandon Scientific Limited for any loss, damage or injury caused by
any errors in, or omissions from, the information given.

All rights reserved. No part of this manual may be reproduced or

recorded, including photo-copying, without the express written
permission of Shandon Scientific Limited.

ii
 AMENDMENTS

Each of these manuals is numbered and listed against a manual holder.

Any further copies required for the service area you are responsible
for will be listed under the same holder with a different sequential
number.

As changes in the machine design necessitate changes in the manual,

amendments will be sent to you to update the text: These amendments
will involve adding and/or changing pages, the amendment log will help
ensure all are up-to-date.

These manuals can only be obtained through Runcorn International

Service Office.

AMENDMENT
LOG

Each time an amendment is issued, fill in the number and date.

Amendment Number Date Signature

740-1-g 1 24.4.91 J J Curran

iii
 INDEX

ChaDter Title Page No

INSTALLATION 1

PROGRAMMING
THE CYTOSPIN 3 2

SERVICEABILITY TEST 8

DAILY AND WEEKLYMAINTENANCE 11

SAFETY 14

STATIC DAMAGETO MOS DEVICES 14

FAULT DIAGNOSIS AND REPAIR 15

FITTING, TESTING AND CALIBRATION 29

Motor 29
Lid 30
Drip tray 31
Damper adjustment 31
Guard barrier 32
Lid catch 32
Out of balance switch 33
Power supply 33
Heatsink 34
Nosecone 34

(a) Main PCB 34

(b) Proximity switch 34
(c) Front panel 35

9 EXPLODEDDIAGRAMSAND PARTS LIST 36

10 LIST OF DRAWINGS 48

11 CIRCUIT DIAGRAMS 49

APPENDIX PRINCIPLES OF OPERATION 51

(1) Overview of System 51

(2) General 51
(3) Power Supply 51
(4) Microprocessor System 52
(5) Display and Keyboard Circuits 52
(6) Motor Drive Circuit 53
(7) Solenoid and Relay Drive Circuits 53
(8) Speed Watchdog and Out of Balance 54
(9) m, m, Hardware Watchdog and
Open Collector Logic 55
(10) Motor, Opto Disc, Lid Lock and
Balance-Switch 55

iv
1 INSTALLATION

1 Unpack the unit and carefully untie the mains lead

(electrical cord).

2 Check the instrument for signs of damage in shipment (broken

glass, broken plastic, dents, etc). If something is missing
or damaged notify Shandon or your supplier immediately.
Quote the order number(s), inspection number, serial number,
date and number of the invoice.

3 Where necessary, fit a suitable plug to the mains lead

(electrical cord). Make sure that the earth wire is
connected and that the mains supply has an earth (ground)
connection.

Instruments supplied to France, Germany and North America

are fitted with plugs during manufacture. Other customers
should connect the mains lead to a suitable rated fused plug
as follows :

Brown wire - Live (L or L2) terminal

Blue wire - Neutral (N or Ll) terminal
Green/Yellow wire - Earth (E or Ground) terminal

Make sure that the voltage rating on the specification plate

at the rear of the Cytospin and on thezinspection tag are
the same as the mains supply rating.

Switch the instrument on by pressing the power switch.

4 Press the ‘Open Lid I button and open the safety cover. In
the event of a power failure, the safety cover cannot be
opened using the ‘Open Lid’ button. The small white cap on
the left-hand side of the instrument should be removed. A
pencil or rod pushed into the hole and pressed firmly will
open the safety cover. Remove the sealed head and set it on
the bench beside the instrument. Remove all internal
packing.

5 Unpack the basic kit and check against the items list in the
Operator Manual.
 2 PROGRAHHING
THE CYTOSPIN 3

This section illustrates how the Cytospin 3 is programmed and how programs may be stored, viewed and run.
Please go through each example completely to gain a full understanding of how to effectively use your
unit. Ensure that the Cytospin lid is closed before running this unit.

(a) Entering and Running a Program

Speed required = 1500 rpm, Time required = 5 minutes, Acceleration rate = low.

Speed Time Accel Audible

Operation Display Display Display Signal

1 Switch 'ON' 0 0 HIGH 3 Set Audible

2 Press 'SET TIME' 0 0 HIGH valid key tone
3 Press ‘5' 0 5 HIGH valid key tone
4 Press 'ENTER' 0 5 HIGH valid key tone
5 Press 'SET SPEED' 0 5 HIGH valid key tone
6 Press ‘1' 1 5 HIGH valid key tone
N
7 Press ‘5' 15 5 HIGH valid key tone
8 Press ‘0' 150 5 HIGH valid key tone
9 Press ‘0' 1500 5 HIGH valid key tone
10 Press 'ENTER' 1500 5 HIGH valid key tone
11 Press 'LOW' 1500 5 LOW valid key tone

The Cytospin 3 is now programmed. This program may now be initiated, ie run (Operation step 12)
and/or it may be stored in memory with a designated program number (see (b) below).

12 Press 'START' 0 5 LOW valid key tone Lid Locked

13 Acceleration Period O-1500 5 LOW Lid Locked
14 Once Speed Attained 1500 5-o LOW Lid Locked
15 Deceleration Period 1500-O LOW Lid Locked
16 Head Stops Rotating 0 : LOW 3 Set Audible Lid Unlocked
17 After 3 Set Audible Signal 1500 5 LOW Lid Unlocked
 (b) Storing (Saving) a Program in Memory

Program Number 1 designated

Program Speed Time Accel
Operation Display Display Display Display

1 Press ‘SAVE’ 0 1500 5 LOW

2 Press Program ‘1’ 1 1500 5 LOW
3 Press ‘ENTER’ 1 1500 5 LOW

Program Number 1 is now entered into the memory. This may be done either before or after.operation
Step 12 above. (Not during running of the Cytospin).

(c) Running a Program From Memory

Program Number 1
Program Speed Time Accel
Operation Display Display Display Display

1 Press ‘LOAD’ 0 - previous program entered -

W
2 Press Program ‘1’ 1 1500 5 LOW
3 Press ‘ENTER’ 1 1500 5 LOW
4 Press ‘START’ 1 0 5 LOW

Program Number 1 has now been recalled from memory and initiated or run.

(d) Viewing Previously Entered Program(s) ‘.

The operator having stored several programs in memory (up to 9) may wish to later view these
previously entered programs to inspect speed/time/accel parameters to act as a reminder in selecting
the desired one.

Let us assume that Section 2 (a) and (b) have been followed entering 9 different programs in memory,
and that the desired parameters are speed: 800 rpm, time: 8 minutes, accel: med, but the actual
program number cannot be immediately remembered by the operator.
 Program Speed Time Accel
Operation Display Display Display Display

1 Press ‘LOAD’ 0 0 0 HIGH

2 Press Program ‘1’ 1 1500 5 LOW
3 Press Program $2’ 2 800 8 MED

As you can see in this example, having sequenced through the above steps, Program Number 2 contains
the desired operational parameters (speed/time/accel) and can now, if desired, be run. Simply
follow operational section 2 (c) 3 through 4.

GUIDANCENOTES

1 To Repeat a Particular Program

The operator has the choice of either,

(a> storing a program in memory for future use and subsequently running this program from memory,
as detailed in section 2 (b) and (c) or

(b) simply closing the lid following the previous run (with new samples correctly loaded) and
pressing ‘START’ - as detailed in Section 2 (a) steps 12 - 17 inclusive.

2 To Alter a Program Mode Parameter (by using the ‘CANCEL’ button)

(a) Press ‘CANCEL’ after ‘SET TIME’ or ‘SET SPEED’ to cancel time or speed previously entered into
memory. Please note it is essential to then select alternative speed/time parameter(s).

(b) Alternatively pressing ‘CANCEL’ systematically after having selected the ‘ENTER’ button for a
time/speed parameter, will revert both the speed and time displays to zero.

Pressing the ‘CANCEL’ button again will cause the speed/time/accel displays to revert back to the
previous program parameters entered.
3 If Program Number 1 is required but the Lid is not closed correctly:

Speed Time Accel Audible Lid Alarm

Operation Display Display Display Signal Lock LED Display

1 Press 'START' 1500 5 LOW - Unlocked Lid Lock

& Open Illuminated
2 Close Lid 1500 5 LOW - Unlocked Lid Lock No Longer
Illuminated
3 Press 'START' 0 5 LOW - Locked Lid Lock No Longer
Illuminated

From this point follow steps 12 - 17 in Section 2 (a).

4 If Program Number 1 is required but the centrifuge head has been loaded incorrectly and is out of
balance

Speed Time Accel Audible Lid Alarm

Operation Display Display Display Signal Lock LED Display

1 Close lid 1500 5 LOW Unlocked

2 Press 'START' 0 5 LOW Locked
3 Acceleration Period 'OUT OF 0 5 LOW Alarm Locked Balance
BALANCE' detected Illuminated
4 Deceleration Period O-1500 5 LOW Alarm Locked Balance
'Illuminated
5 Head Stops Rotating o-1500* 0 LOW Alarm Locked Balance
Illuminated
6 Switch 'OFF'
7 Switch ‘ONI 0 LOW 3 set Unlocked
8 Re-select Program 1 1500 LOW Unlocked
9 Correct 'OUT OF BALANCE' 1500 LOW Unlocked
10 Press 'START' 0 LOW Unlocked
11 Follow Section 2 (a) step 12 on.
5 Invalid Soeeds Lower Than 200 mm

The Cytospin 3 sample chamber assembly will not 'TILT' into the upright position unless a speed of
200 rpm is achieved. The program memory will reject as INVALID any speed lower than 200 rpmr as
illustrated in this example when 190 rpm is entered.

Speed Time Accel Audible

Operation Display Display Display Signal

1 Press 'SET TIME' 0 HIGH valid key tone

2 Press ‘5' 0 HIGH valid key tone
3 Press 'ENTER' 0 HIGH valid key tone
4 Press 'SET SPEED' 0 HIGH valid key tone
5 Press '1' 1 HIGH valid key tone
6 Press '9' 19 HIGH valid key tone
7 Press ‘0' 190 HIGH valid key tone
8 Press 'ENTER' 0 HIGH invalid speed

Action to be taken i select and enter a value of 200 rpm or higher.

6 Invalid Speeds Higher Than 2000 rpm

The Cytospin 3 program memory will reject any speed entered greater than 2000 rpm. As an
illustration, examine the following procedures when 2010 rpm is programmed.

Speed Time Accel Audible

Operation Display Display Display Signal

1 As Number 5 above Steps l-4 0 5 HIGH valid key tone

2 Press ‘2' 2 5 HIGH valid key tone
3 Press ‘0' 20 5 HIGH valid key tone
4 Press ‘1' 201 5 HIGH valid key tone
5 Press ‘0' 2010 5 HIGH valid key tone
6 Press 'ENTER' 0 5 HIGH invalid speed

Action to be taken - select and enter a value of 2000 rpm or lower.

7 Invalid Speed/Time Parameters

The Cytospin 3 will not start without a valid speed and time entered and displayed. Similarly a
program cannot be saved if the speed and time are not valid.

8 ‘!’ Light (Overspeed Indication/Rotor Jam)

The ‘! ’ light is illuminated in the event that speed control becomes irregular during a run, due to
electrical interference or instrument malfunction.

Similarly should the sealed head assembly fail to start rotating due to mechanical or electrical
malfunction, then the ‘!I light will be illuminated after approximately 3 seconds. An audible alarm
signal will sound, and the ‘drive’ to the sealed head motor will be disconnected.

Turn the power off, and then refer to Section 2, No 4, Steps 7, 8, 10, 11.
SWVICEABILITYTEST

Introduction

This procedure describes the test which can be carried out, at

any time, on the Cytospin 3, to ascertain if the machine is
functioning correctly. In the following tests a sealed head is
fitted without cell or cell holders.

Instruments Required

One Multimeter, one 1OOOV Megger, one Stopwatch.

Insulation Tests

Before applying power to the machine the following insulation

tests must be carried out. Using the multimeter, check the
resistance path between the earth wire and any metal parts of the
machine, this resistance is to be less than ORl. Connect the
1OOOV megger between the live and neutral wires on the mains
power lead. With the power switch in the off position, the
resistance should be greater than 100 megohms. Short the live
and neutral wires of the mains power lead together. With the
power switch in the ON position the resistance between the
live/neutral wires and the earth should be greater than 25
megohms. If above is correct, apply power to machine.

Procedure

When power is applied to the Cytospin 3 thejdisplay panel should

initialise to the following (if not, consult Fault Diagnosis
Section and/or Principles of Operation) and the alarm should
sound for three seconds.

Program Display 0
Speed Display 0
Time Display 0
HIGH ON
MED OFF
LOW OFF
STOP ON
START OFF
BAL OFF
! OFF
LID LOCK OFF
OPEN LID OFF

Keyboard Tests

Press SET SPEED, check speed 0 flashes.

Press 0 - 9 in turn and check that digit appears on the right

hand side of the speed display and "rolls round".

Press CANCEL, check that display reads 0 flashing.

Press CANCEL, check that display reads 0.

8
Press SET TIME, check that display reads 0 flashing.

Press 0 - 9 in turn and check that digit appears on right-hand

side of time display and "rolls round".

Press CANCEL, check that display reads 0 flashing.

Press CANCEL, check that display reads 0.

Press LOAD, check program 0 flashes.

Press 0 - 9 in turn and check that the digit appears in the

program window.

Press CANCEL, check program 0 flashes.

Press CANCEL, check program displays 0.

Operation Tests

Press SET SPEED.

Press 2000.

Press ENTER, display should read 2000.

Press SET TIME.

Press 10.

Press ENTER, display should read 10.

Press START.

The Start LED should now come on and the Stop LED go off. The
sealed head will begin to rotate and accelerate quickly until the
SET SPEED is reached. The speed display will now read the actuaZ
speed of the sealed head in rpm and time display will begin to
count down towards 0.

When the time display reaches 0 the sealed head will come to a
halt. The Start LED will go off and the Stop LED will come on.
The speed display will now read the SET SPEED (ie 2000) and the
time display will reset from 0 to 10. The audible alarm will
sound at the end of operation.

Press SET SPEED.

Press 200.

Press ENTER, display will now read 200.

Remove the cells and cell holders from the sealed head, replace
the sealed head in the machine and close the lid.

Press SET TIME.

Press 30.

Press ENTER, the display should read 30.

Press HIGH ACCEL, the HIGH ACCEL LED should come on and the LOW
ACCEL LED should go off.

Press START, the sealed head should begin to rotate.

Using the stopwatch, check that the timer counts down in 1 minute
intervals. Check that the speed display remains at 2000 rpm and
that the head remains quiet throughout the 30 minute program.

When the timer reaches 0 the machine should stop and the audible
alarm sound. The time display should reset to 30.

If the Cytospin 3 passes the above functional tests it can be

considered to be serviceable. If the machine fails to pass any
of the tests then reference must be made to the appropriate part
of the servicing manual.

10
DAILY AND WEEKLYMAINTENANCE

CLEANINGAND STERILISING THE CYTOSPIN 3

All the components of the Cytospin 3 which are likely to become

contaminated have been designed to be easily cleaned using mild
detergent solutions, and then sterilised using a wide variety of
agents.

In the interest of laboratory safety, Shandon recommends that

suggested sterilisation procedures are adhered to.

All the components of the sealed head, the centrifuge bowl liner,
safety cover and front panel, are designed to be easily cleaned.

Each component part is dealt with individually and if the

instructions are followed, no damage will occur. This is
particularly important , since any chemical attack on the
components of the sealed head or hinged safety cover will weaken
<;-iz -p:?rial and create a serious hazard.

When handling, cleaning or sterilising any component, it is

advisable to wear gloves as they offer protection against
infection and chemical effects on the skin due to the detergent
and/or disinfectants being used. After cleaning and sterilising
any component, it should be examined for damage. If in doubt as
to its state, it should not be used on the centrifuge until
inspected by your Safety Officer.

IMPORTANTNOTE

If you are in doubt as to the type of disinfectant you

are using, many brands do not declare the nature of the
active ingredients , check with the manufacturer before
using the solution with any component part of the
Cytospin 3.

HYPOCHLORITEbased disinfectants should NEVER be used to

sterilise centrifuges of any type.

FRONT PANEL

Frequency: Weekly and after any spillage.

Clean : Use a damp cloth to clean panel; do not use abrasive

powders, these will damage the surface of the panel.

Sterilise: Use 2% glutaraldehyde prepared according to manu-

facturer’s instructions.

Notes: a) Never clean the front panel with Xylene, Toluene or

similar solvents.

b) The front panel can be polished if required with a

liquid or spray furniture polish.

11
HINGED SAFETY COVER

Frequency: Daily and after any serious spillage.

Clean: Use warm soapy water, DO NOT use abrasive powders, these
will scratch the surface and make it more difficult to
clean and also create a potential source of cross-
contamination.

Sterilise: Use 2% glutaraldehyde prepared according to manufac-

turer's instructions.

Note: DO NOT clean the hinged cover with any solvents - these
could weaken the safety cover.

BOWLLINER

Frequency: Daily and after any serious spillage.

Clean: Use warm soapy water, do not use abrasive powders, they
will create scratches which may harbour micro-organiss
and make sterilisation difficult.

Sterilise: Use 2% glutaraldehyde prepared according to manufac-

turer's instructions.

SEALED HEAD ASSEMBLY

Frequency: Daily and immediately after any-serious spillage.

Sterilise: The head assembly can be sterilised as a whole unit b

autoclaving at 121OC (250'F) for 15 minutes. It is
necessary to unlock the lid so that the pressure ins&&z
the head will be equalised with those in the autoclave
and to allow the free entry of steam into the interior
of the head.

Clean: After sterilisation of the whole head, all components

should be removed, washed in warm soapy water and dri&
in an oven. (Maximum temperature 65'C or 149'F) befon
re-assembly.

SEALED HEAD BASE

Frequency: Daily and immediately after any serious spillage.

Sterilise: Autoclave at 121OC (250OF) for 15 minutes. Wipe with2%

glutaraldehyde or a phenolic disinfectant prepared
according to manufacturer's instructions.

Clean:_ After sterilisation clean using warm soapy water, riiae

in clean water and dry. DO NOT USE hard brushes to
clean this component.

12
SEALED HEAD SLIDE CLIP ASSEMBLYSUPPORTPLATE

Frequency: Daily.

Sterilise: As Sealed Head - Base.

Clean: As Sealed Head - Base.

Note: This component is easily removed from the Sealed Head

Base by removing two thumb screws. This enables the
under surface of the support plate to be cleaned easily
and also facilitates cleaning of the inside surface of
the sealed head base. The support plate can be totally
immersed in 2% glutaraldehyde disinfectant if required-

SEALED HEAD LID

Frequency: Daily.

Sterilise: As Sealed Head - Base.

Clean: As Sealed Head - Base.

Note: The silicone rubber seal around the rim of the lid cau
be easily removed and replaced. It is advisable to
remove this seal at weekly intervals to enable all the
surface of the lid to be cleaned. A new seal should Be
fitted at intervals of 12 months.

CYTOCLIP

Frequency: Daily.

Sterilise: Autoclave at 121OC (250'F) for 15 minutes or immerse in

2% glutaraldehyde solution according to manufacturer's
instructions.

Clean: Rinse in clean water before use and hot air dry.

Note: It is advisable to ensure that the pressure spring is

unlocked, releasing any pressure from the-spring durQg
sterilisation. It is also important to ensure that ube
face of the slide clip is clean as any raised particles
could cause microscope slides to break under the appl&ed
'gr force.

SAMPLECHAMBER

Frequency: After use.

13
5 SAFETY

1) There are potentially lethal voltages in the Cytospin 3. Take

all necessary precautions when working on live machines.

2) Beware of mechanically moving parts. They may move unexpec-

tedly under the control of the microcomputer system.

6 STATIC DAHAGETO HOS DEVICES

Some of the components used on the Cytospin 3 electronics are MOS

devices and are sensitive to static damage.

1) Take all necessary precautions when handling.

2) Treat all unknown devices as MOS.

3 Remember, statically damaged devices may take weeks or months

to fail.

DO NOT TAKE CHANCES

1.4
7 FAULT DIAGNOSIS AND REPAIR

If the Cytospin 3 stops working it will be because either a fault

condition has occurred or a safety circuit has operated. Refer
to the list of fault conditions below until you find the symptoms
of your fault. Turn to the relevant page and work through the
possible causes, methods of diagnosis and solutions. To retest
the Cytospin after each repair or replacement, follow the
serviceability test.

If the symptom of your fault is not listed below refer to the

principles of operation and the relevant circuit diagram.

Fault Condition Page

1 Front Panel LED's not illuminated 16

2 SPEED, TIME, PROG, ACCEL and STOP display LED's 18

will not initialise on 'Power On'.

3 STOP, START, HIGH and LOW ACCEL LED's will not 19

initialise on 'Power On'.

Cytospin 3 will not accept program commands. 20

Lid lock LED lights, when Start button pressed. 21

Motor will not run. 23

Balance LED lights. 24

Balance LED fails to light (with out-of-balance 26

sealed head).

9 ! LED lights and motor stops (failure of motor 27

speed control loop).

10 Lid solenoid not working. 28

THERE ARE POTENTIALLY LETHAL VOLTAGESIN THE MACHINESO CARE MUSTBE

TAKEN WHENTESTING OR MAKINGADJUSTMENTSON LIVE MACHINES.

15
FAULT CONDITION 1: FRONT PANEL LED’S NOT ILLUMINATED

Possible Cause Diagnosis Solution

1.1 Mains supply has failed. Check mains supply with suitable Wait for supply to return or find
meter or by examining another an alternative power source.
appliance. Retest.

1.2 Incorrect mains power Check that supply for 240V Cytospin If not, connect to correct supply
supply l
is between 220V and 25OV, for 1lOV and retest using serviceability
machine between 1OOV and 120V. test.

1.3 Plug and fuse FSl not Check plug. Check that fuse FSl is If not, fix properly or replace.
intact. in place and intact (Drawing Retest. If fuse blows again,
E740-l-l), 0.8 amp for 24OV, investigate short circuits, earth
2 amp for 1lOV. (ground) leakage etc.

1.4 Faulty cable, switch or (a) Check there is mains input If not, inspect the cable and
wiring. voltage at the input terminals wiring. Replace as necessary.
of SW1 (Drawing E740-l-l). Retest.

(b) Ensure SW1 is closed and check If not, replace SWl. Retest.
if mains input voltage is present
at output side of SW1 (Drawing
E740-l-l).
FAULT CONDITION 1: FRONT PANEL LED'S NOT ILLUMINATED

Possible Cause Diagnosis Solution

1.5 Power supply from Check at socket for the following

transformer plate. nominal voltages (Drawing E740-l-l)

(a) AC Voltages (a) If not, test transformer

Between pins 1 and 2: 24V AC windings for continuity (Drawing
Between pins 5 and 6: 15V AC E740-l-1). Typical resistances
Between pins 10 and 12: 8V AC for windings are:
Between pins 11 and 12: 8V AC Primary 16.0 R
Secondary 24V 0.4 R
Secondary 15V 1.4 R
Secondary 8-0-8V 0.5 9

(b) DC Voltages (b) If not, check across bridge

Between pins 7 and 8: 24V DC rectifier Bl for 24V DC and 24V
Between pins 9 and 8: 24V DC AC (Drawing E740-l-l).

If correct, inspect and test

associated plugs and'wiring for
open or short circuits, earth
(ground) leakage etc (Drawing
E740-1-1). Replace as necessary.
Retest.
FAULT CONDITION 2: SPEED, TIME, PROG, ACCEL AND STOP DISPLAY LED'S WILL NOT INITIALISE ON 'POWER ON'

Possible Cause Diagnosis Solution

2.1 Incorrect power supplies Check that 12V, 5V, -5V and OV DC If not, check through relevant
to mains PCB. rails are available on Main PCB. supply circuit and replace as
necessary (Drawing F740-1-2,
E740-l-l). Retest.

2.2 Mains PCB faulty. Check main PCB by replacing with If correct, leave new PCB in
serviceable PCB. Before replacing place.
PCB check that the correct voltages
are available. (Drawing E740-l-l)
(Refer to voltage table, Principles
of Operation).
FAULT CONDITION 3: STOP, START, HIGH AND LOWACCEL LED’S WILL NOT INITIALISE ON ‘POWER ON’

Possible Cause Diagnosis Solution

3.1 Fault on main PCB. Check main PCB power supplies If correct, leave new PCB in
(voltage table, Principles of place.
Operation). If correct, replace
main PCB with serviceable main
PCB (after checking voltages).

3.2 LED’s not functioning. Press the Act switch and check If correct, test the LED’s which
that the Lo Act LED lights. are not functioning. Replace as
Program the Cytospin and press necessary. Retest.
the Start switch, check that the
start LEd lights and that the
Cytospin operates correctly.

3.3 Wiring or connection Check associated wiring and If not, replace as necessary.
fault. connectors for continuity, open Retest.
or short circuits, earth (ground)
leakages (Drawing F740-1-2,
E740-l-l).
FAULT CONDITION 4: CYTOSPIN 3 WILL NOT ACCEPT PROGRAMCOMMANDS

Possible Cause Diagnosis Solution

4.1 Main PCB. Replace PCB with serviceable If now correct, leave new PCB in
PCB. Before replacing PCB check place.
that supply voltages are correct
(voltage table, Principles of
Operation).

4.2 Fault on Cytospin 3 Check keyboard by fitting front If.not, replace as necessary.
keyboard (front panel). panel to serviceable Cytospin. Retest.

4.3 Fault on molex Check connectors and wiring. Replace as necessary. Retest.
connections between
main PCB and keyboard
FAULT CONDITION 5: LID LOCK LED LIGHTS WHENSTART BUTTONPRESSED

Possible Cause Diagnosis Solution

5.1 Lid not closed properly. Check lid fully closed. If correct, retest.

5.2 Lid lock switch (SW5) Check operation of SW5 as follows: If not, replace SW5 as necessary.
faulty. With the lid fully closed, connect Retest.
a continuity tester between pins 11
and 12 of socket 3 (Drawing E740-1-1)
and check for continuity. Manually
operate the solenoid (Sol 1) until
the lid catch opens and the lid
raises slightly (you should be able
to hear the catch opening). There
should now be an open circuit between
pin 11 and 12 of socket 3.

5.3 Magnetic proximity switch Check the operation of SW3 as If not, replace SW3 as necessary.
(SW3) faulty. follows: Retest.
Place a magnet on the top trim in
such a position that it will close If correct, operate Cytospin with
the proximity switch. Connect a external magnet in position. If
continuity tester between pin 7 on machine now functions correctly
PL2B and pin 11, PL4A (Drawing (ie defect not apparent) this
E740-1-1) continuity. Remove magnet indicates that lid magnet is not
and check that there is now an open closing proximity switch.
circuit between these pins.
 FAULT CONDITION 5: LID LOCK LED LIGHTS WHENSTART BUTTONPRESSED

Possible Cause Diagnosis Solution

5.4 Fault on lid lock circuit Check associated wiring Replace as necessary.
wiring. (Drawing E740-l-l).

5.5 Main PCB fault. Replace main PCB with serviceable If correct, leave new PCB in place.
PCB. Before replacing PCB check
that correct voltages are
available (voltage table, Principles
of Operation).
lo
N
FAULT CONDITION 6: MOTORWILL NOT RUN

Possible Cause Diagnosis Solution

6.1 Motor fault. Check coil of motor for continuity If not, replace motor as necessary.
(Drawing E740-l-l). Retest.

6.2 Incorrect power supplies Check power supply circuits and If not, replace as necessary.
to motor. components (Drawing E740-1-1). Retest.

6.3 Fault on Relay A. Check Relay A for correct operation If not? replace as necessary.
(Drawing E740-1-1). Retest.

6.4 Transistor (TRl) fault. Check transistor TRl for correct If not, replace as necessary.
operation (Drawing E740-l-l). Retest.

6.5 Main PCB Replace main PCB with serviceable If correct, leave new PCB in place.
PCB. Before replacing PCB check
that correct voltages are available
(voltage table, Principles of
Operation).
FAULT CONDITION 7: BALANCELED LIGHTS

Possible Cause Diagnosis solution

7.1 Sealed head loaded Check that sealed head is loaded If correct, retest.
incorrectly. correctly.

7.2 Vibration switch (SW4) Adjust vibration switch (refer to If correct, retest.
incorrectly adjusted. adjustment procedure Testing and
Calibration section).

7.3 Vibration switch (SW4) Check operation of SW4 as follows: If not, replace SW4 as necessary.
faulty. Using a continuity tester, test for Retest.
open circuit between pins 10 and 12,
socket 3 (Drawing E740-1-1). With
tester still connected physically,
tilt the Cytospin to the side in
order to close SW4. Viewing the
Cytospin from the front, SW4 is
positioned laterally at the rear of
the machine.
FAULT CONDITION 7: BALANCELED LIGHTS

Possible Cause Diagnosis Solution

7.4 Main PCB Fault Check main PCB by replacing with If correct, leave new PCB in place.
serviceable PCB. Before replacing
PCB check that correct voltages are
available (voltage table, Principles
of Operation).
FAULT CONDITION 8: BALANCELED FAILS TO LIGHT (with sealed head out of balance)

Possible Cause Diagnosis Solution

8.1 Vibration switch (SW4) Adjust vibration switch (refer to If correct, retest.
incorrectly adjusted. adjustment procedure Testing and
Calibration section).

8.2 Vibration switch (SW4) or Check switch and wiring (refer to If not, replace as necessary.
associated wiring faulty. Drawing E740-l-l and Fault Retest.
Diagnosis, paragraph 8.3).

8.3 Faulty LED. Test LED using diode tester If not, replace as necessary.
(Drawing E740-1-2). Retest.

8.4 Main PCB fault. Check main PCB by replacing PCB If correct, leave new PCB in place.
with serviceable PCB. Before
replacing PCB check that correct
voltages are available (voltage
table, Principles of Operation).
 FAULT CONDITION 9: ! LED LIGHTS AND MOTORSTOPS (failure of motor speed control loop)

Possible Cause Diagnosis Solution

9.1 Fault on control opto With ‘Power On’ (but start switch If not, check opto transistor,
sys tern. not pressed) connect voltmeter disc encoder, wiring etc. Replace
between pins 1 and 2 on sensor PCB as necessary. Retest.
plug (Drawing E74@-1-1). Slowly
turn the sealed head assembly
(or drive boss) by hand and check
that the output from the opto is
present.

N
-4
9.2 Main PCB fault. Check main PCB by replacing with If correct, leave new PCB in place.
serviceable PCB. Before replacing
PCB check that correct voltages
are available (voltage table,
Principles of Operation).
FAULT CONDITION 10: LID SOLENOIDWILL NOT OPERATE

Possible Cause Diagnosis Solution

10.1 Fault on transistor T12 With solenoid button pressed check (a) If correct, check solenoid and
and T13 (main PCB). output of T12 and pin 1 PL2B main PCB catch assembly. Replace as
(Drawing F740-1-2). necessary. Retest.

(b) If not, check transistor T12 and

T13, microswitch and wiring.
Replace as necessary. Retest.
8 FIT'UXG, TESTING AND CBLIBRATION

All internal screws and nuts to be fixed with Loctite Screwlok, WITH
THE EXCEPTION OF ANY IN CONTACT WITH PLASTICS, where Araldite (eg
lid) must be used.

(1) MOTORREMOVALAND REPLACEMENT

a> With the access door open and the sealed head removed,
isolate the machine from the electrical supply.

b) Loosen the M3 grub screw in the side of the taper, pull the
drive taper off the motor shaft. Remove “V” ring seal from
the motor shaft. Do not lose the key in the motor shaft,
it should be kept for fitting into the new motor shaft.

c> Remove the four countersunk screws securing the steel

fixing plate to the bowl and motor. The bowl is now
released from the motor. (Retain these special screws for
refitting new motor).

d) Remove the outer two countersunk screws securing the front

of the bowl to the instrument case. (These screws are
under the silicone rubber seal behind the nosecone trim;
care should be taken not to damage the seal).

e> Close and secure the access door. (A protecting cover, ie

foam, should be placed over the door).

f> Remove the four screws securing the nosecone to the plinth
(base) and carefully pull the nosecone away from the case.
The nosecone will fit face down in front of the plinth
(place some protection on the bench for the control panel).
Disengage the three connectors , remove the M5 earth nut and
leads. The nosecone can now be taken from the machine.

g) Turn the machine upside down and remove the six securing
screws from the plinth (base). The plinth can now be
tilted up and to one side for removal of the M5 earth nut
and leads (up and to the left when the machine is upside
down and facing front). The plinth can now be removed.

h) Loosen the two screws securing the encoder bracket to the

guard barrier and slide the bracket back as far as
possible, retighten the screws. Release the M2.5 grub
screw fixing the encoder disc to the motor shaft and
carefully pull the disc off the shaft, after removing the
grip ring from the motor shaft.

i> Remove the nut retaining the motor leads to the guard
barrier.

j) Remove the four countersunk screws that fix the motor

housing to the guard barrier, the motor will now be free to
remove from the guard barrier once the leads have been
desoldered. (When fitting the new motor ensure correct
wiring of motor leads).

29
 k) The motor is now removed and the motor housing released
from the motor by removal of the four countersunk screws.

Fitting the new motor is generally the reverse of the removal,

but the following points should be noted:

a) It is important to use the original screwlok screws in and

around the motor. LOCTITR SEX1 LOCK HUST NOT COKR INTO
CONTACTWITH ANY PLASTIC OR PAINTED COXPONENT.

b) When re-fitting the encoder disc and bracket, the disc

should turn freely near the centre of the gap between the
encoder heads, and the bracket should be fixed so that the
encoder assembly is as close as possible to the edge of the
disc without any risk of contact between the two. Refit
the grip ring to the motor shaft.

c) When re-fitting the plinth (base).do not tighten the six

screws.

The machine is turned upright and the access door opened

(manually operate the solenoid to release the catch).

Replace the PTFE seal along the top edge of the nosecone.
Care must be taken when re-fi t ting the nosecone that the
two lugs fit smoothly under the bowl seal. Loosely fit the
two countersunk screws.. Fit the nosecone so that the front
lower edge of the nosecone lines up with the front top edge
of the plinth (base). Fix the nosecone using the four
screws. Do not over-tighten the two countersunk screws in
the bowl. Pull the case forward to meet the nosecone and
tighten the six screws..

d) Fit the “V” ring seal to the motor shaft with the lip
facing down. Push the seal just past the keyway in the
shaft. Fit the key into the keyway on the motor shaft.
The drive taper is then fitted and fixed 1 mm from the
steel fixing plate (the seal will be pushed down into
position by the drive taper).

Reconnect the electrical supply and test the functions of the

instrument.

(2) LID REMOVALAND REPLACEMENT

a) Before isolating the machine from the electrical supply

open the lid.

b) Remove the right hand screw (looking from the front of the
machine) fastening the lid hinge to the pivot (do not lose
the spacing washer or the shakeproof washer). Remove the
two M3 countersunk socket screws holding the right hand
hinge plate to the lid frame and remove the hinge plate.
(Support the lid while removing these parts).

30
 c) The lid can now be moved to the left and the left-hand
pivot will slide out of the bearing block. The lid can WV
be dismantled.

d) Replacing the lid is the reverse of the removal.

e) Forward adjustment of the lid is achieved by slackening the

two hinge pivot screws and sliding the lid forward or back.

f) To raise or lower the lid loosen the four screws securing

the two bearing blocks at the rear of the machine (two
either side) and adjust the lid height accordingly.
Remember to also adjust the plunger height in order to keep
the lid on the seal correctly.

(3) DRIP TRAY AND LID SEAL REMOVALAND REPLACEMENT

-

a) Before isolating the machine from the electrical supply

open the lid.

b) Remove the seventeen countersunk screws retaining the seal

and the drip tray to the case of the machine.

c) Remove the drive taper from the motor shaft (M3 grub screw)
retain the key from the motor shaft. Remove the ‘V’ ring
seal from the motor shaft.

d) Remove the four countersunk screws in the clamp plate and

remove the plate. The drip tray can now be removed.

e) Inspect the rubber extrusion on the lid of the guard

barrier and replace if necessary. When removing only the
lid seal, the seventeen screws need to be removed and a sew
seal can be fitted around the drip tray, ensure that the
lower lip of the seal fits over the case of the machine and
that the seal sits properly on the drip tray.

Replacement of the drip tray is the reverse of the removal.

The motor shaft ‘V’ ring seal must be fit.ted with the lip
facing down, push the seal just past the keyway in the
shaft. Fit the key into the keyway on the motor shaft, the
drive taper is then fitted 1 mm from the steel clamp plate
(the seal will be pushed down into position by the drive
taper). Secure the drive taper.

(4) LID DAMPERADJUSTMENT

a) Remove the drip tray as described in Section 3.

b) The damper is found on the right hand side of the main

case.

c) To increase damping , screw the nylon adjuster into the

damper body.

31
 d) i To remove the damper from the machine, remove the
right hand hinge screw fixing the hinge plate to the
pivot.

ii Remove the two M5 nuts securing the damper body to the

case. The damper assembly can then be removed.

iii The link arm is separated by removal of the retainer

on the damper arm.

(5) GUARDBARRIER REMOVALAND REPLACEMENT

a) Refer to section (l), step a and step c.

b) After closing and protecting the lid, turn the machine

upside down and remove the ten (10) M4 screws securing the
plinth (base) to the case and nosecone. The plinth will
then lift off to one side of the machine.

c) Remove the M5 earth nuts, leads and plugs joining the

plinth to the case and remove the plinth.

d) Disconnect the wiring to the motor, optics and out of

balance switch.

e) Remove the eight springs supporting the guard barrier (loop

a piece of cable through the outer loop of the spring,
restrain the machine with one hand and pull the spring free
with the other).

f) The guard barrier can now be removed.

g) Replacement is the reverse of the removal.

(6) LID CATCHMECHANISM

a) If the whole assembly is to be removed, the guard barrier

has to be removed first (see section (5))

b) Once the guard barrier is extracted, remove the four (4) M3

screws securing the aluminium angle carrying the catch
assembly. The assembly can now be inspected.

c) The catch assembly, the microswitch and the solenoid can be

removed without removing the aluminium angle but care must
be taken when refitting that the components are correctly
fitted.

d) In order to function correctly the solenoid plunger must

always be fully home when the solenoid is energised, also
the catch must clear the lid plunger with sufficient
clearance (until it is flush with the side of the lid
plunger hole). Once the solenoid is fully home and the
catch is flush with the hole sides, the catch must then be
free to move 0.5 to 1.0 mm further out.

32
(7) OUT OF BALANCESWITCH ADJUSTMENT

a) Remove the round access plug on the back of the

instrument.

b) The out of balance switch can be seen through this access

hole.

cl the switch is mounted on a rectangular plate held by two

screws. Loosen both of these screws.

d) One of the screws is in a slot. Slide the rectangular

plate counter-clockwise a small amount - approximately loo.
Retighten both screws.

e) Test for out of balance using all 12 chambers and clip

assemblies. Once you are satisfied the machine does not go
out of balance, proceed to step f).

f) Using 10 chambers and clip assemblies, load the sealed

head (see Operator Manual). Ensure that the two positions
left vacant are not directly opposite each other.

Select high acceleration and 2000 rpm and start machine.

The out of balance should operate at a point between 1000
and 1500 rpm.

Repeat using low acceleration.

g) Make certain the screws are tight; replace the access plug
and retest.

(8) POWERSUPPLY REMOVALAND REPLACEMENT

a) Isolate machine from the electrical supply.

b) Protect the lid (use foam etc).

c) Turn the machine upside down and remove the ten (10) M4
screws.

d) Lift the plinth up and to the left side (looking from the
front). Disconnect the 3 plugs and ground leads, and the
plinth is then free to remove.

e) Disconnect the electrical leads from the power supply, and

supporting the plinth and power supply, remove the four
fixing screws from the underside of the plinth. The power
supply is now free of the plinth.

f) Replacement is the reverse of the removal instructions.

33
(9) HEATSINK

Two different types of heatsink may be found

a) Internal
b) External

a) Internal

i> Remove the plinth [as in Section 8, paragraphs a)

to d)].
ii) Disconnect and remove the heatsink retaining nuts,
desolder the transistor leads and disconnect the
wiring fixed to the plinth. The plinth can now be
removed.

b) External

i> Remove the two outer M3 screws and extract the

heatsink from the machine.

ii) The transistor mounting body is secured using two M3

screws. Remove these and the transistor can be seen.
Desolder and remove from the machine.

iii) In both cases the replacement is the opposite of the

removal.

(10) NOSECONEASSEMBLY

Removal and Replacement Main PCB

a) Remove the nosecone, (see Section 1, paragraphs a, d

and f).

b) Disconnect the main PCB from its retaining pillars, and

partially extract it through the back of the nosecone.
Take care not to damage any of the components on the board.

c) Disconnect PCB plugs.

Remove the main PCB.

Replacement is the reverse of removal.

NB: Replace the PTFE seal along the top edge of the nosecone.

Proximity Switch

a> Remove the nosecone (Section 1, paragraphs a, d and f).

b) Remove the main PCB.

34
c> Remove the two M4 screws retaining the nosecone trim and
remove the trim.

d) Remove the two M3 nuts and remove the proximity switch.

Note: It is possible to remove the trim.without removing the

main PCB using a screw retaining screwdriver, but great
care must be taken to avoid damage to any components on
the PCB.

Replacement is the reverse of removal, but when positioning the

proximity switch it must be adjusted so that the machine will
not start when the lid is slightly open. The switch must be
out of the magnetic field before the lid plunger releases the
lid catch microswitch.

NB: Replace the PTFE seal along top edge of the nosecone.

Front Panel

a) Remove the nosecone (Section 1, paragraphs a, d and f).

b) Remove the main PCB.

c) Remove the eight (8) M3 nuts and remove the front panel
from the front of the nosecone.

Replacement is the reverse of removal.

NB: Replace the PTFE seal behind the front panel. Also
replace the PTFE seal along the top edge of the nosecone.

35
 CYTOSPIN 3
PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

1 59930096 Top Cap Locking Pin MA 1000 07P 1

2 59930097 Handle - Sealed Head Assembly 3

3 PO7595 Seal - Silicone - Sealed Head 1

4 59930095 Locking Pin - Locking Device 5

5 PO7437 Top Cover Mouldings 1

G,
4
6 59930094 Main Body - Locking Device 5

7 PO9638 Ball Bearing %" dia (2) 1

8 PO9698 Thumb Nuts (2) 1

9 59920047 Cell Plate 3

10 PO7431 Seal V Ring 1

11 59930093 Hub Sealed Head 4

 PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

12 PO6007 Grub Screw M3 x 8 (2) MA 1000 07P 1

13 PO7525 0 Ring 1

14 PO7516 Seal 1

15 59920046 Inner Bowl 1

16 59930063 Drive Boss 4

2;;:
17 PO7432 V Ring - Motor Shaft 1

18 59930068 Fixing Collar 4

19 PO7596 Moulded Seal 1

20 59920033 Drip Tray 2

21 PO7512 Key 2 mm x 2 mm x 12 1

22 PO7402 Motor 1
PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

23 59920034 Outer Bowl MA 1000 07P 7

24 PO9610 Spring Retention Pin (8) II

1

25 PO7441 Spring Barrier (8) 11 1

26 59920038 Bracket - Vibration Switch 11 2

27 59930072 Clamp Plate 11

1

28 PO7524 Mercury Position Sensor II

1

29 59930070 Support Pillar (3) II

2

30 59930064 Support Collar II

5

31 59930059 Carrier Plate II 2

32 PO7444 Encoder Disc 11

1

33 59930060 Clamp Plate II

1
 PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

34 PO7522 Grip Ring MA 1000 07P i

II
35 59920127 Mounting Bracket 1

36 PO7467 Opt0 Interrupter (2) 1

36 59920131 Opto Interrupter PCB 2

37 59930154 Angle Bracket Right 1

&
37 59930155 Angle Bracket Left 1

38 59910131 Motor Suppresser Plate 2

39 PO6979 12 Way Receptacle Housing 1

40 59930051 Mounting Block 1

41 PO4249 5 mm Nut 1

41 59930162 Locknut 3
 PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

42 59930161 Plunger Lid Catch MA 1000 07P 2

43 74010051 Lid Panel Assembly 11

1

44 74030053 Screw - Hinge Plate (2) II

1

45 74030052 Hinge Plate - Right Hand II

1

45 74030051 Hinge Plate - Left Hand II

1
s
46 59930071 Tapping Strip (2) 11
4

47 59930055 Hinge Pivot I,

2

48 59930152 Hinge Block - Right II

3

48 59930167 Hinge Block - Left II

3

49 59920032 Damper Arm Assembly II

5

50 PO7430 Rubber Buffer II

1
PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

51 PO9696 Damper Rod MA 1000 07P 1

Damper I, 1
52 PO7426

Plug Cover (Mercury Switch) II 1

53 PO7763

Maincase ,I 1
54 74020030

Guide Bracket 11 1
55 59930044

59930160 Mounting Angle II 1

56

57 59930042 Mounting Plate Solenoid II

1

Plug 318" Black II 1

58 PO6741

Spring Solenoid II 1
59 PO7526

60* PO6148 Solenoid II

5

* NOTE: Items 60 and 61 are bonded together and should not be ordered separately.
PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

61* 59930043 Adaptor - Solenoid - Spindle Catch MA 1000 07P 3

62 , 59910121 Catch Assembly II

4

63 PO7764 Microswitch I,
1

64 59930037 Microswitch Bracket ,I

3

67 59920128 Proximity Switch Assembly II

1

68 PO2969 Grommet ,I
1

69 P11993 Proximity/Switch Pillar ,I

1

70 PO7476 Voltage Regulator ,I

1

76 74030070 Top Trim I,

1

II
77 74020061 PCB Mainboard 1

74020064 EPROMfor above II

1

* NOTE: Items 60 and 61 are bonded together and should not be ordered separately.
 PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

79 74020060 Nosecone MA 1000 07P i

81 74030020 Front Panel Cytospin 3 11 1

82 PO6979 Receptacle 12 Way II 1

83 PO6976 Plug Housing 12 Way II 1

84 PO9407 Transistor PMD16K60 ‘1 1

z
85 PO9254 Transistor Mounting Kit '1 1

86 59930005 Transistor Mounting Block I' 1

89 59920009 Heatsink 1' 3

90 74020001 Plinth II 1

91 PO1517 Clamp Plate - Mains Lead II 1

92 PO7882 Fuse Holder II 1

PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

93 PO4654 Fuse Marking Plate MA 1000 07P 1

94 P11130 Fuse 0.8 Amp Slow Blow 1

PO7884 Fuse Cap 240V FEIT 031-1663 1

PO7828 Fuse 2 Amp Slow Blow 1

PO7883 Fuse Cap 1lOV FEIT 031-1661 II

1

95 PO6755 Input Block Mains II

1

II
96 59930009 Terminal Block Pad 1

97 PO7155 Relay 60 73 IML II

1

‘1
98 PO6102 Capacitor Clip 1

99 PO7462 Capacitor 4700 yF II

1

‘1
100 59920041 Tagboard Assembly 240V 5
PARTS LIST IDENTIFICATION

Ident No Part No Description From Ser No Issue

100 59920043 Tagboard Assembly 1lOV MA 1000 07P 4

101 P11944 Rocker Switch II 1

102 PO6979 Receptacle 12 Way ‘1

1

103 P11109 Mains Filter ‘1

1

104 59930001 Transformer Plate 4

105 PO7807 Diode Bridge Rectifier 1

106 59930116 Transformer II

1

107 59930171 Spacer 2

108 PO7602 Black Neoprene Buffer 1%" x 'h" Thick 1

59930156 Hinge Adjustment Screw 2

RECOHMENDEDPIRSTLINE REPAIRKIT - CYTOSPIN 2

Part No Description

59920041 Tagboard Assembly 240V

59920043 Tagboard Assembly 1lOV
59920131 Opto PCB
59930094 Main Body - Locking Device
59930095 Locking Pin -. Locking Device
59930096 Top Cap - Locking Pin
59930097 Handle - Sealed Head
59930116 Transformer
74010004 Transformer Plate Assembly 1lOV
74010005 Transformer Plate Assembly 240V
74020061 PCB Mainboard
74020062 Programmed PAL
74020063 Programmed PAL
74020064 Programmed Eprom
74030020 Front Panel
PO5606 Proximity Switch (SW3)
PO6148 Solenoid
PO7155 Relay - LO 73 IMO
PO7402 Motor - Type 55M Spec
PO7431 Seal - V Ring
PO7432 Seal - V8 - Motor Shaft
PO7437 Top Cover Moulding
PO7444 Disc Encoder
PO7462 Capacitor 4700 nF
PO7467 Opt0 Interrupter
PO7476 Voltage Regulator
PO7516 Seal
PO7524 Switch Mercury (SW4)
PO7525 '0' Ring
PO7595 Seal - Silicone - Sealed Bead
PO7596 Seal Moulded
PO7763 Plug and Cover - Mercury Switch
PO7764 Microswitch (SW5)
PO7807 Diode Bridge Rectifier
PO9254 Mounting Kit
PO9351 Brush Assembly - Motor
PO9407 Transistor - Darlington - PMB 16K60
PO9638 Ball Bearings
P11109 Mains Filter
P11944 Switch - Rocker (SWl)

47
10 LIST OF DRAWINGS

Page No

Cytospin 3 llOV-250V Circuit Diagram E740-1-l 49

Circuit Diagram - Main PCB F740-1-2 50

Note: The PCB used on the Cytospin 3 is a common board, not all
component locations will be used.

48
 110
? a I n I
I’ I I I - II

CIRCUIT
DIAGRAM-MAIN
PCB F740-1-2
SHANDON SOUTHERN PRODUCTS LTD
CYTOSPIN III

40 41 42 43

28 26 27

108
 PLGh
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6--m)-- GY GY
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I
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DIAGRAM-CYTOSPlN(llO-250V)
 APPENDIX

PRINCIPLES OF OPERATION

This description uses drawing numbers:

F740-1-2 Circuit Diagram Main PCB

E740-l-1 Circuit Diagram Cytospin (110-250V)

(1) OVERVIEWOF SYSTEM

The Cytospin 3 is a bench-mounted centrifuge with a range of

speeds from 200 - 2000 rprn. The time and speed required are
entered and displayed digitally by means of a keyboard and LED
displays.

The Cytospin 3 incorporates a number of safety features:

1 Overspeed detection
2 Out of balance detection
3 Electronic failure detection

The Cytospin 3 has been designed to provide maximum protection to

the operator from biological specimens.

(2) GENERAL (Ref F740-1-2)

The Cytospin 3’s electronic circuit occupies one PCB in the

nosecone assembly. It is connected to the main instrument by
meansof a detachable loom. This PCB replaces the earlier
versions as fitted to the Cytospin 2 and is not retrofittable.

The PCB is partitioned into well defined functional areas which

are described below. The PCB is factory calibrated and requires
no analogue setting-up procedure.

(3) POWERSUPPLY

Mains power is supplied to the machine via SW1 (the main on/off
switch) which is mounted on the plinth. The internal circuitry
is protected by FSl, a slow-blow fuse, 0.8A for 250V operation -
2A for 1lOV operation. Interference protection, both from inter-
ference getting into the machine from the external mains supply
and vice-versa, is provided by FLl.

The low voltage supplies for the machine are derived from Tl,
this is a toroidal transformer to minimise space and temperature
rise. It has two primaries which are connected in series for
240V operation or in parallel for 1lOV operation, as shown on
drawing E740-1-l. Two voltage dependent resistors Sl, S2 are
connected across the primaries to suppress any voltage spikes
which are not attenuated sufficiently by FLl. Tl has three
secondaries, 24V AC at 4A rms, 8-0-8V AC at 2A rms and 15V AC at
0.58 rms.

51
 The 24V winding is used to supply power to the motor, lid
solenoid and motor control relay, it is rectified by diode
bridge Bl and smoothed by Cl, a 4700uF capacitor. Both Bl and
Cl are situated on the transformer plate assembly in the
plinth.

From the transformer, 8V AC is fed to the main PCB, rectified

by Dll, D12 and C36, then regulated by VR3 to provide a +5V
supply * The 12V AC winding is unused on the Cytospin 3.

(4) MICROPROCESSOR
UNIT

The microprocessor system on the Cytospin 3 is centred around

IC6, a Hitachi HD63A03Y 8-bit microcomputer. The clock signal
for the microcomputer is provided by the 4.9152 Mhz quartz
crystal at X2 together with capacitors C245 and C25.

The system ROM is contained in IC7 while the PLA at IC8 is used
for address decoding. In the event of a power failure, all
stored programs are retained in memory by the EEPROMat IC4.
Power failure detection and software watchdog facilities are
provided by the MAX690 at IC2 and the PLA at IC5 respectively.

(5) DISPLAY AND KEYBOARDCIRCUITS

The display and keyboard driving is multiplexed. The LED’s

1 - 8 are scanned by a walking zero on port six of the
microcomputer, as are the seven segment displays. This allows
for increased intensity of display for reduced current
consumption.

The walking zero is used to switch on one of the transistors

T16 to T23. The transistor supplies the common anode of the
seven segment display and another LED with 5V. The other end
of the LED’s in the seven segment display and the separate LED
are pulled low through RN2 and R41. This is done selectively
by the pattern being fed through from the darlington driver
chip ICll.

ICll is memory mapped and driven by the processor to power the

correct display segments. All the seven segment displays are
mounted in tuned pin sockets for easy replacement.

The keyboard is of standard touchpanel construction being

arranged in three rows of eight. The rows of eight going
vertically down the keyboard are scanned by port 6 of the
microcomputer at a frequency of about 100 Hz, therefore
response is immediate.

52
 The three horizontal rows are monitored by IC13 which is memory
mapped. Connection to the keyboard is through a single in line
18-way Berg connector (PL3) via a short tail to the top of the
keyboard.

LED 9 is driven by IC14 and supplied from pull up resistor R40.

(6) MOTORDRIVE CIRCUIT

The motor control output provides a regulated current to the base

of the motor drive power darlington transistor TRl.
v - 1.5
This current is approximately -CD amp, where V,, is the
300
voltage at the collector of transistor Tll and 300 is the
value in ohms of R28. V,, is controlled by a unity gain long-
tailed pair amplifier made up from T8, T9 and TlO. The
amplifier compares the collector voltage V,, with an input
taken from a reservoir capacitor C26 to adjust the base current
of Tll. The voltage appearing at capacitor C26 is controlled
by a series of one millisecond pulses which are generated at
pin 14 of the microprocessor. These pass through inverter IC8c
and a resistor R27, which provide the correct charge level and
rate control for the capacitor.
(7) SOLENOID AND RELAY DRIVE CIRCUITS

The solenoid which is activated to allow the lid to be opened and

the relay which is driven to allow motor current to reach the
motor both operate from +24 volts and have similar drive
circuits. Because of the inductive nature of both devices,
by-pass diodes D6 and D7 are fitted to re-circulate switched
current.

The solenoid is activated by taking IC6 pin 23 low and the relay
by taking IC6 pin 15 low. In each case this switches on a pnp
transistor (T15 and T13) connected to +5 volts, which provides
base current to an npn transistor (T14 and T12), which itself
powers the device. The base current to the drive transistor in
each case can be clamped to ground to prevent the transistor
switching on. For the relay, this is done using an open
collector handgate IC17 driven by KEs and mRT providing a safety
interlock.

740-l-91
53
 The lid release solenoid can only be driven under software
control. Should the software control fail, the open lid key will
not operate. In an emergency the lid release solenoid can be
opened manually. This is done by turning off the mains supply
and inserting a thin screwdriver through the hole provided in the
left side panel. The hole is covered by a plastic cap.

(8) SPEED WATCHDOG

AND OUT OF BALANCE

The out of balance detector mercury switch is used to generate an

mAT by being latched on the NM1 decode/general interface PAL (IC5).
This will cause the drive to the motor to be cut via IC17 and the
plinth relay to release, causing regenerative braking.

In addition, the BAL LED will light showing an extreme fault

condition. The Cytospin 3 has to be switched OFF then ON to
recover from the situation. Other signals that cause the same
sequence of events include hardware overspeed. The ability to
detect imminent power fail is not used.

The Cytospin 3 has a software based overspeed detection circuit

that will cut the drive, cause regenerative braking and light the !
LED. Recovery again is by switching OFF then ON.

The microprocessor monitors the angular speed by monitoring the

output of the slotted opto mounted beneath the motor. This signal
is fed to the board PL2a pin 3 then squared up using the Schmitt
inverter IC18 and fed into the microprocessor’s interval timer
input on IC6 pin 9.

Running in parallel to this software overspeed detector is a true

hardware overspeed detector. This circuit is comprised of IC3 and
its associated discrete analogue components. Essentially the
circuit is a frequency to voltage converter.

The squared up opto signal is differentiated using C18, IC3 and R3

to produce a pulse for every edge detected. This pulse is then
rectified using D2 and used to charge C19, should the speed be too
high Cl9 will be unable to discharge to a low voltage. The average
of the voltage on Cl9 is generated across C20 using R4, it is this
voltage that is a reflection of the speed.

The final stage using POT2 and IC3 is a comparator producing a low
when overspeed is detected. This signal is latched by the PAL IC5
creating an NHI and the usual machine shutdown. POT2 is used to
set the voltage threshold and hence the frequency threshold. Due
to the variation in component value and the variation in the Vf of
D2, the circuit must be calibrated using a known frequency of 2.6
KHz on the spin input to produce the overspeed warning.

740-l-91
54
 The circuit once calibrated should never require recalibration. A
feature of the software is the anti-jam mechanism. This checks to
see if any pulses are being generated on the rotor speed opto
interrupter input line. If the microprocessor expects rotor
rotation because power is applied to the motor and none is
detected, then drive power will be removed after a period of
approximately 4 seconds. This is to prevent motor damage if the
motor is jammed, but also acts as a safeguard against opto
interrupter failure.

(9) NMI, RESET, HARDWARE

WATCHDOG
AND OPEN COLLECTORLOGIC

The FU3%has the same action on the open collector logic as the NRT
signal. The two signals being added together in IC8 to produce the
CVT signal driving IC17, cutting drive and driving the plinth relay
RLl via T14 to cause motor drive isolation and regenerative
braking.

The m signal will be produced by the MAX690 (IC2) in one of two

situations. Should the power fail, prior to the ILogic levels being
affected, at 4.5 volts the microprocessor is reset and will not be
released from reset until such time as the power rail has risen
above 4.5 volts and a further 1.6 seconds has elapsed.

The NRT signal will also be produced should a watchdog signal not
be produced on IC6 pin 22. This signal is an indication that the
software is operating normally, its absence indicates software
corruption.

(10) MOTOR, OPT0 DISC, LID LOCK AND BALANCESWITCH

The motor used is a 24V permanent magnet type, mounted in line with
the rotor assembly. The power to the motor is controlled by relay
RLA and transistor TRl (E740-l-l), these components are mounted on
the plinth. When the machine is stopped or de-energised, power to
the motor is removed and it is short circuited by contacts RLAl,
RLA2 (connected in parallel) of relay RLA. Thus, if the machine is
stopped the motor is regeneratively braked (ie it acts like a
generator with a short circuit output). When the machine is
started RLA is energised and power can be supplied to Ml via TRl.
Because this transistor under certain conditions is called upon to
dissipate up to 6OW, it is mounted on a heatsink which projects out
of the rear of the machine. The control signal for TRl is derived
from the main PCB and is described above.

55
The lid of the Cytospin is locked by means of a mec.hanical latch
which can only be released by means of a solenoid Sol 1 (E740-l-l),
the control signal for which comes from the main PCB; So as to
inform the microprocessor control system if the lid is locked or
not, two switches are incorporated in the circuit, SW3 and SW5.
Switch SW3 is a reed relay proximity switch which is activated by a
magnet in the lid. The switch itself is mounted in the trim on top
of the nosecone. As the lid is closed the magnet is brought closer
to SW3 and when it is within range the switch will close. To
ensure that the lid is fully locked, a microswitch is incorporated
in the lid latch assembly (SW5), this switch detects if the lid
latch is not fully home. Since SW3 and SW5 are in series, a signal
(5V) will only be present to the main PCB when the lid is fully
shut (F740-l-l).

In the event that the head is loaded with a non-balanced load and
the machine started, an out of balance switch is incorporated to
inform the microprocessor to switch the machine off. This switch,
SW4, consists of two contacts situated above a small bead of
mercury. If the machine vibrates the mercury is agitated and
bridges the gap between the contacts, this signal is latched on the
main PCB and stops the machine. Because the mechanics of the
suspension system exhibit different characteristics depending upon
whether the rotor is accelerating or decelerating, the switch is
then taken out of the circuit by RLA3 when the motor is turned off.

56