Operating Instructions

ROBERT RIELE GmbH & Co KG

Software Version 2.3

Documentation Version 4.2007
4040e_23.doc / 05.04.07

SYMBOLS

The packaging material, the type plate on the instrument, and the manual may contain the following symbols or
abbreviations:

Manufactured by:

This product fulfills the requirements of Directive 98/79/EC on

in vitro diagnostic medical devices.

In vitro diagnostic medical device

Caution (refer to accompanying documents)!

Please refer to safety-related notes in the manual accompanying this device.

Please consult instructions for use

Description of technical specifications according to DIN 58 960, part 4

Biohazard
Samples containing material of human origin must be treated as potentially infectious.
The relevant laboratory guidelines on safe use must be observed.

Symbol for the marking of electrical and electronics devices according to § 7 ElektroG

No special protection against penetrating moisture (IP = International Protection)

IP XO
Order number
REF
Serial number
SN

INSTRUMENT APPROVALS

The Photometer 4040 meets the requirement stated in Directive 98/79/EC of the European Parliament and the
Council of the European Union (EU) on in vitro diagnostic medical devices. Furthermore, the Photometer 4040
is manufactured according to the special safety requirements for IVD medical devices stated in DIN EN 61010,
testified by TÜV Rheinland Group.

The Photometer 4040 fulfils the EMC immunity requirements for laboratory use equipment according to the
EMC standard EN 61326.

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SAFETY INFORMATION

User qualification
Only appropriately trained operators are qualified to operate the device.

Environmental conditions
The Photometer 4040 is approved for indoor use only.
For further environmental conditions see chapter 11.1.

Patient ambience
The Photometer 4040 may not be used in the patient ambience.

Electrical Safety
This device was examined and left the factory in perfect technical condition. To preserve this and protect safe
and faultless operation, the user must follow the orders and remarks of this operating manual.

Connect the device to grounded power outlets only. All peripheral devices that are connected to the Photometer
4040 must comply with safety standard EN 60950. Before connecting read the documentation of the peripheral
devices.

Opening covers or removing parts of the instrument, except where this can be achieved manually without the
use of any tool, may expose voltage-carrying components. Connectors can be live, too. Never try to maintain or
repair an open instrument which is carrying voltage.

Repairs at the device including replacement of the Lithium battery may be carried out only by authorized
specialist staff. Through improper repairs the warranty extinguishes, and the user can be heavily jeopardized.

If suspected the device can no longer be operated safely, switch it off and take steps to ensure that no one will
subsequently attempt to use it.

Electromagnetic waves
Devices that emit electromagnetic waves may affect measured data, or cause the Photometer 4040 to
malfunction. Do not operate the following devices in the same room where the Photometer 4040 is installed:
mobile phone, transceiver, cordless phone, and other electrical devices that generate electromagnetic waves.

Reagents
Regarding reagents follow the safety as well as the operating instructions of the manufacturers.
Pay attention to the currently valid German “Gefahrstoffverordnung” (GefStoffV)!

Biological safety
Liquid waste is potentially biologically hazardous. Always wear gloves if handling those materials. Do not touch
parts of the device other than those specified. Consult the laboratory protocol for handling biohazardous
materials.
Pay attention to the currently valid German “Biostoffverordnung” (BioStoffV)!

Spillings and cleaning

If a sample is spilled on the device, wipe up immediately and apply disinfectant.

Waste
Handle liquid waste properly, according to legislation on water pollution, and on the treatment of drainage and
waste matter.

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MANUFACTURER’S WARRANTY

ROBERT RIELE GmbH & Co KG warrants Photometer 4040 against defects in material and workmanship.
For further information contact the local distributor.

WASTE MANAGEMENT NOTE

At the end of the life or utilization time the device and the accessories can be given back to the manufacturer
with costs for an environmental waste disposal. The previous professional decontamination has to be proved
with a certificate.

Address of the manufacturer:

ROBERT RIELE GmbH & Co KG

Kurfuerstenstrasse 75-79
D-13467 Berlin
Germany

Phone: +49 (0)30 404 40 87

Fax: +49 (0)30 404 05 29
E-Mail: info@riele.de
http://www.riele.de

QUALITY MANAGEMENT SYSTEM

ROBERT RIELE GmbH & Co KG maintains a quality management system according to DIN EN ISO 9001 and
DIN EN ISO 13485 certified by TÜV Rheinland Group.

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CONTENTS

1 INTRODUCTION TO PHOTOMETER 4040 ................................................7

2 INSTALLATION .............................................................................................8
2.1 DELIVERY .......................................................................................................................................... 8
2.2 PREPARATION FOR INSTALLATION ............................................................................................. 8
2.3 INSTALLATION .................................................................................................................................. 8
2.4 LOADING PRINTER PAPER............................................................................................................ 9

3 OPERATING ELEMENTS ...........................................................................10

3.1 FRONT ..............................................................................................................................................10
3.2 BACK.................................................................................................................................................10
3.3 WORKING AREA .............................................................................................................................11
3.4 DISPLAY ...........................................................................................................................................11
3.5 KEYBOARD.......................................................................................................................................11
3.6 FUNCTIONS OF THE KEYS ..........................................................................................................12

4 SELECTION OF MEASURING PROGRAMS ...........................................13

5 MEASUREMENT WITH BASIC METHODS..............................................14
5.1 GENERAL NOTES ...........................................................................................................................14
5.1.1 Fundamental to the handling ....................................................................................................14
5.1.2 Fundamental to the heating ......................................................................................................14
5.1.3 Fundamental to the inputs ........................................................................................................14
5.1.4 Fundamental to the methods with standard ... ..........................................................................15
5.1.5 Fundamental to the methods with multi-standards ...................................................................15
5.1.6 Fundamental to bichromatic measurements ….........................................................................15
5.1.7 Fundamental to the Kinetic… ....................................................................................................16
5.2 MODE FUNCTIONS .........................................................................................................................17
5.3 ABBREVIATIONS..............................................................................................................................18
5.4 SURVEY OF THE METHODS ........................................................................................................19
5.5 DESCRIPTION OF METHOD COURSES ......................................................................................20
5.5.1 Concentration Measurement with Factor ..................................................................................20
5.5.2 Concentration Measurement with Factor and Reagent Blank ...................................................20
5.5.3 Concentration Measurement with Factor and Sample Blank ....................................................21
5.5.4 Concentration Measurement with Factor, Sample Blank and Reagent Blank ...........................21
5.5.5 Concentration Measurement with Standard ..............................................................................22
5.5.6 Concentration Measurement with Standard and Reagent Blank...............................................22
5.5.7 Concentration Measurement with Standard and Sample Blank ................................................23
5.5.8 Concentration Measurement with Standard, Sample Blank and Reagent Blank.......................24
5.5.9 Fixed Time with Factor..............................................................................................................25
5.5.10 Fixed Time with Standard ........................................................................................................26
5.5.11 Kinetic with Factor ...................................................................................................................27
5.5.12 Kinetic with Standard ...............................................................................................................28
5.5.13 Transmission Measurement.....................................................................................................29
5.5.14 Difference with Factor ..............................................................................................................30
5.5.15 Measurment with 3 wavelengths..............................................................................................31
5.6 Printer ON / OFF................................................................................................................................32
5.7 Serial interface utilities .......................................................................................................................32
5.8 Changing Sample Numerator ............................................................................................................33
5.9 Multi-standard tools ...........................................................................................................................33
5.10 Patient data management................................................................................................................38
5.10.1 Working with patients data within a course ..............................................................................38
5.10.2 Patient data management utilities............................................................................................38
5.11 Lamp protection ...............................................................................................................................40

6 USER GUIDANCE AT DISPLAY ..............................................................41

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7 METHOD EDITOR.......................................................................................43
8 UTILITY PROGRAMS .................................................................................47
8.1 SELECTION OF UTILITY PROGRAMS .........................................................................................47
8.2 DESCRIPTION OF UTILITY PROGRAMS .....................................................................................48
8.2.1 Dark level adjustment................................................................................................................48
8.2.2 User name.................................................................................................................................48
8.2.3 Error list.....................................................................................................................................48
8.2.4 Deleting method ........................................................................................................................49
8.2.5 Language selection ...................................................................................................................49
8.2.6 Beep ON / OFF .........................................................................................................................49
8.2.7 Temperature control ON / OFF .................................................................................................49
8.2.8 Temperature calibration ............................................................................................................50
8.2.9 AD-Transducer readings ...........................................................................................................50
8.2.10 ADC dark current values..........................................................................................................50
8.2.11 Display test ..............................................................................................................................51
8.2.12 Printing device configuration....................................................................................................51
8.2.13 Real time clock ON / OFF ........................................................................................................52
8.2.14 Filter wheel test........................................................................................................................52
8.2.15 Temperature correction............................................................................................................52
8.2.16 Printing user defined methods .................................................................................................53
8.2.17 Laboratory name......................................................................................................................53

9 MAINTENANCE ...........................................................................................54
9.1 CLEANING INSTRUCTION ...............................................................................................................54
9.2 CALIBRATING MEASURING SYSTEM ............................................................................................54
9.3 CHANGING RIBBON.........................................................................................................................54
9.4 CHANGING PAPER ROLL ................................................................................................................54
9.5 REPLACEMENT OF LINE FUSES .................................................................................................55

10 ERROR MESSAGE / CORRECTION ........................................................56

10.1 GENERAL NOTE............................................................................................................................56
10.2 ACOUSTIC ERROR MESSAGES .................................................................................................56
10.3 PLAINTEXT ERROR MESSAGES ................................................................................................56
10.4 CODED ERROR MESSAGES.......................................................................................................56

11 TECHNICAL DATA .....................................................................................59

11.1 ENVIRONMENTAL CONDITIONS .................................................................................................59
11.2 MINIMAL OPERATION QUALITY .................................................................................................59
11.3 TYPE PLATE ..................................................................................................................................59
11.4 SHORT SPECIFICATION...............................................................................................................60
11.5 CERTIFICATES...............................................................................................................................61

12 ACCESSORIES AND SPARE PARTS......................................................64

13 METHOD LIST ............................................................................................65
13.1 BASIC METHODS ..........................................................................................................................65
13.2 LIST OF USER SPECIFIC METHODS........................................................................................66

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1 INTRODUCTION TO PHOTOMETER 4040

This device, Photometer 4040, a programmable photometer for manual applications, is designed for In Vitro
Diagnostic (IVD) use by qualified laboratory staff.

The device is useable e.g. for wet clinical-chemical analyses. The solution can be measured in glass or
disposable cuvettes placing one after the other. To make the measuring solution the optimal temperature, the
use of glass cuvettes is recommended.

The solution is quickly and exactly heated to 37 °C in the measuring cell as well as in the incubation block with 2
positions.

For different measuring methods several programmed methods with open parameters are available (chapter 5 -
MEASUREMENT WITH BASIC METHODS and chapter 13 - METHOD LIST).

15 basic methods with open parameters are available in the device (chapter 5 - MEASUREMENT WITH
BASIC METHODS and chapter 13 - METHOD LIST).
Beside this up to 230 free methods - built up on the basic methods - can be created and stored with a method
editor (chapter 7 - METHOD EDITOR) by the user. The list of methods can be printed out (chapter 8.2.16 -
Printing user defined methods).

Up to 50 nonlinear calibration curves with maximal 20 sets of points can be stored (chapter 5.9 - Multi-
standard tools).

For the different measuring tasks the device has a capacity for up to 250 pre-programmed test variants with
fixed parameters.

Normally the device has optical filters of 340, 405, 492, 546, 578 and 623 nm wavelength. Three more filters like
e.g. 670 nm can be built in.

Normally the device is operated by a membrane keyboard. External operation is possible by a serial data
interface (chapter 5.7 - Serial interface utilities).

The result is shown on a 32-digit lighted display. The device has a built-in printer and a serial data interface.

According to a GLP conformal documentation the name of lab and user can be printed out as well as transferred
to EDP (chapter 8.2.17 – Laboratory name and 8.2.2 - User name).

Results of patients can be managed in the memory of Photometer 4040 (chapter 5.10 - Patient data
management).

Photometer 4040 supports the download of the operating system using FLASH MEMORY technology. So new
features of software development can easily be updated without any manipulation on hardware level (see
chapter 5.7 -Serial interface utilities: Download to FLASH MEMORY).

In the unlikely case of a fault which cannot be located or remedied by the user, the device can be checked by
built-in utilities for function test and faulty remedy. This guarantees fast, accurate help and easy repair.

For a listing of used terms and text see chapter 5.3 - ABBREVIATIONS.

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2 INSTALLATION
2.1 DELIVERY
After careful unpacking check if the device is in perfect condition. Check that the box contains all the following
items:

1 Axis printer paper

1 Dust cover
2 Fuses for line power
1 Mains cable
2 Printer paper
1 Ribbon band printer
1 Top cover small for printer

In the event of damage during transit, contact the local distributor. Store the packaging carefully for the
case of a possibly necessary return.

2.2 PREPARATION FOR INSTALLATION

The device must be placed on a stable, level surface. The input air at the bottom and the waste air at the back
plate may not be obstructed to guarantee the ventilation of the device.

If the device has been exposed to marked changes in temperature and/or humidity, wait for it to acclimatize
sufficiently before operating.

2.3 INSTALLATION
The Photometer 4040 operates at any line voltage between 90 VAC and 264 VAC at 50/60 Hz without adjustment.
The device plug of the mains cable must be put into the mains cable socket at the back of the device and the
mains plug of the mains cable must be put into a grounded mains socket.

For combined operation with external devices (PC, printer) read the relating documentation. Pay atten-
tion to the above safety instructions. Switch these devices on at first.

Switch the photometer on by pressing the mains switch at the back of the device.

(c) ROBERT RIELE After switching on Copyright, ...

GMBH & CO KG

(c) ROBERT RIELE Website, ...

www.riele.de

PHOTOMETER 4040 ... Type of device and version number is displayed. Parallel to this, type of device
V2.xa dd.mm.yy D and version number is printed in case of an activated printer.

TEMP. 37C <> Display in case of an activated temperature control. It disappears after reaching
PLEASE WAIT B of 37 °C.
Proceeding to next display with [BREAK] is possible.

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METHOD NO. After this the level for program selection appears.
INPUT 1-250
The program selection is carried out in accordance with chapter 4 - SELECTION
OF MEASURING PROGRAMS

The device is heated up and ready for measurement after around 15 minutes. At first the heating is switched off.
If the user wishes to work later with tempered measuring solution, it is recommended to switch on the heating
now to stabilize itself parallel to the warming up of the device. Switch on the heating directly by utility program
(chapter 8.2.7 -Temperature control ON / OFF) or indirectly by selection of a method with programmed warming
up (for details see chapter 5.1 - GENERAL NOTES).

If errors appear during operation, first of all they have to be confirmed with [ENTER] before remedying (see
chapter 10 - ERROR MESSAGE / CORRECTION).

If required, a dark level adjustment can also be executed at this time according to chapter 8.2.1 - Dark level
adjustment.

2.4 LOADING PRINTER PAPER

At the first putting into operation or when the end of printer paper is color marked, new printer paper must be
inserted:
• Open the printer cover and remove the rest of paper.
• Put printer paper axis into the new printer paper reel.
• Fold the beginning of paper over the corner to an angle of 90° (as shown below).

• Put this tip into the guide below the ribbon cassette.
• Press [LF] several times for line-feed until the paper has passed the tear-off edge.
• Put printer paper reel into the axis guide.
• Take paper through the crack of the printer cover and close the printer with the cover.

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3 OPERATING ELEMENTS
3.1 FRONT

display printer cover

lid

working area

keyboard

3.2 BACK

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3.3 WORKING AREA

lid

working area

cuvettes

3.4 DISPLAY

3.5 KEYBOARD

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3.6 FUNCTIONS OF THE KEYS

NORMAL OPERATION
Key/-combination Function Chapter

[0]...[9], [•] Keys for numeric input 5.5/ 7/ 8

[MODE] MODE function for alterations of adjustment 5.2 / 8
[LF] Line feed of paper 2.4
[+], [-] Sign change
Choice keys for methods, MODE functions and utilities 4/7/8
Detailed printout after carried out Kinetic measuring
[BREAK] General break function 4/7/8
[ZERO] Setting to zero 5.1/ 5.5
[ENTER] Confirmation key 5.1/ 5.5
[RESULT] Triggering measuring 5.1/ 5.5
[WASH] (No function)

MODE FUNCTIONS
Key/-combination Function Chapter

[MODE] [4] Printer ON / 5.6

[MODE] [5] Multi-standard tools 5.9
[MODE] [7] Serial interface utilities 5.7
[MODE] [8] Changing Sample Numerator 5.8
[MODE] [9] METHOD EDITOR 7
[MODE] [MODE] UTILITY PROGRAMS 8
[MODE] [LF] SERVICE TOOLS Service
manual

TEXT EDITOR LEVEL

Key/-combination Function Chapter

[7 / ABCD] Input of A...B...C...D 7

[8 / EFGH] Input of E...F...G...H 7
[9 / IJKL] Input of I...J...K...L 7
[4 / MNOP] Input of M...N...O...P 7
[5 / QRST] Input of Q...R...S...T 7
[6 / UVWX] Input of U...V...W...X 7
[1 / YZ12] Input of Y...Z...1...2 7
[2 / 3456] Input of 3...4...5...6 7
[3 / 7890] Input of 7...8...9...0 7
[0 / / # % -] Input of /...#...%...- 7
[WASH / A↔a] Change between capital and small characters 7
[• / Chr.→] Change of the cursor to the next input position 7

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4 SELECTION OF MEASURING PROGRAMS

METHOD NO. A method can be called directly in the level for program selection by input of the
INPUT 1-250 method number (see also chapter 2.3 - INSTALLATION and chapter 13 -
METHOD LIST). Confirm with [ENTER].

It can also be selected indirectly by repeated pushing of [+/-], so that the methods
are scrolled till the required method is reached.

No. Program group Chapter Description

1 ... 250 Direct method selection 5 MEASUREMENT WITH BASIC METHODS

6 USER GUIDANCE AT DISPLAY
13 METHOD LIST

[MODE] Utilities 8 UTILITY PROGRAMS

[MODE]

A combination of direct and indirect selection of a method is possible.

[BREAK] has a general break function for all methods and subroutines back to the level for program selection.

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5 MEASUREMENT WITH BASIC METHODS

5.1 GENERAL NOTES

The device offers user guidance in the display by a combination of plaintext and short terms (chapter 6 - USER
GUIDANCE AT DISPLAY). If not already automated, e.g. at selection of a basic method, the demanded action
is displayed, and parameters are queried.

In principle, reports and inputs to the method have to be confirmed with [ENTER]. With [BREAK] all methods
can be broken off. New start according to chapter 4 - SELECTION OF MEASURING PROGRAMS. Measuring
is generally triggered by [RESULT], zero measuring by [ZERO].

5.1.1 Fundamental to the handling ...

• The lid over cuvette compartment has to be closed before all measuring.

• Deviations from normal operation, caused by the device or by the user, e.g. the programmed
upper limit was exceeded by the measurement, are notified by “ERROR”. They have always to be
confirmed with [ENTER] (chapter 10 - ERROR MESSAGE / CORRECTION).

5.1.2 Fundamental to the heating ...

• The switching state of the heating is parameter of a method. Therefore switching ON or OFF of the
heating is carried out via selection of a method.

• The heating can be switched on/off manually.

TEMP. 37C <> Display in case of an activated temperature control. It disappears after
PLEASE WAIT B reaching of temperature.
Proceeding to next display with [BREAK] is possible.
The heating can also be switched on/off manually by utility program 7 (chapter 8.2.7).

• After switching on the heating it lasts around 10 minutes till a constant temperature of 37 °C is
reached.

• Indicator for the heating process, and so for a not yet constant temperature, is a flashing cursor at the
utmost right position in the upper display line and an asterisk (*) at the utmost right position in the
corresponding print line.

• For a quick mode of operation all temperature-sensitive samples, reagents and washing solutions
should be externally temperated by Incubator T12 (REF 500-002) or a water bath.

5.1.3 Fundamental to the inputs ...

• The entry format of the factor or the standard with sign determines the output format of the result
regarding the number of the fractional digits.

• Instead of being measured, the reagent blank can also be entered via the keyboard or put on zero.

• In favor of a homogeneous solution the input of a delay before a measuring is possible at all methods.

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5.1.4 Fundamental to the methods with standard ...

• Each measurement of a standard (calibrator) can be performed as single, double or triple
determination.

After measurement of a standard with e.g. triple determination following is displayed:

0.302 0.299 The first line shows the absorbance 1 and 2 of the standard.
0.303 E/+/0 The second line shows the absorbance 3 of the standard.

With [ENTER] the average of all values is calculated. Values with 0 are
ignored and excluded from the calculation. The resulting factor is
calculated on the average of the standard.

With [+] a selection between the values is done. A black rectangle

marks the actual value (in this case 0.299).

With [0] a value is deleted and excluded from the calculation.

With [RESULT] a measurement is performed.

• The determined resulting factor of a standard measuring is saved together with the corresponding
method number. After renewed selection of a method number the resulting factor is offered as
„Old STD“.

5.1.5 Fundamental to the methods with multi-standards ...

• Linear calibration is used in the case of two calibrators. The assay absorbance at different
concentrations forms a linear plot (chapter 5.9 - Multi-standard tools).

• Nonlinear calibration is used for those sample constituents where assay absorbance at different
concentrations forms a nonlinear but reproducible plot. At least three (maximum 20) calibrators are
required for nonlinear calibration (chapter 5.9 - Multi-standard tools).

5.1.6 Fundamental to bichromatic measurements …

• The calculation procedures based on endpoint measurement (CP 1 to CP 8, CP 13 and CP 14) can be
executed bichromatic. The zero measurement will be done with a wavelength defined as bichromatic.
The bichromatic wavelength might be not included in the standard set of filters. The bichromatic
wavelength can be set after calling a method.

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5.1.7 Fundamental to the Kinetic…

In a kinetic method the sample absorbance is measured several times in pre-established time intervals.
The user can define a delay time and a quantity and duration of time intervals after the delay time (Deltas or ∆t).
At the beginning and at the end of the delay time the absorbance values ABS.1 and ABS.2 are measured
respectively. The difference |ABS.1 – ABS.2| allows the differentiation between normal and abnormal activities.
This is followed by a sequence of measurements in regular time intervals (Deltas or ∆t). An example for a
resulting curve is shown on next figure.

In each time interval (Delta or ∆t) the difference between the relating absorbance values as well as the gradient
of the curve are calculated.
To obtain the alteration per minute ∆AS,Minute the gradients must be averaged. This is done by a simple linear
regression calculation also giving an indicator for the linearity of the test. This indicator is called the coefficient of
correlation R. For practical reasons, the square of the coefficient of correlation R^2 is taken in a Kinetic
calculation. The value of R^2 can vary between 0 and 1. An R^2 value of 1 indicates perfect linearity and a
value of 0 indicates absolute non-linearity. Already values < 0.9 indicate a bad linearity and therefore an
incorrect test.
In practice, linear tests show values of R^2 near to 1. In the example for method 11 (Kinetic with Factor) values
of R^2 ≥ 0,998 are permitted. Results with smaller R^2 values could be caused by temperature instability,
pollution, expired reagents, unfavorable delay time, etc.
For a better monitoring the number of deltas (deltas or ∆t) should be bigger than specified for the manual
procedure. The classic three-minutes-test with three deltas of 60 s can be replaced by 15 deltas of 12s.

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5.2 MODE FUNCTIONS

The mode functions serve for changing definite basic settings during a course of method. It is not necessary to
exit the method and to change to the level of UTILITY PROGRAMS (chapter 8). Also temporary parameters
remain unchanged. Corresponding to these features this group of functions is available only within the repetition
sequence of a course of method (chapter 5.5 - DESCRIPTION OF METHOD COURSES).

Change to the selection level of the mode functions with [MODE].

MODE FUNCTIONS Selection level of the mode functions: Selecting the desired mode function by
direct selection i.e. input of the number key in question (see table) or by scrolling
through all mode functions with [+] ... [+]. Confirm with [ENTER].
Exit the selection level of the mode functions and change back to the course of
method with [BREAK].
After working off a mode function the return is carried out to the course of
method.

MODE FUNCTIONS
Key/-combination Function Chapter

[MODE] [1] Lamp protection 5.11

[MODE] [4] Printer ON / 5.6

[MODE] [5] Multi-standard tools 5.9

[MODE] [7] Serial interface utilities 5.7

[MODE] [8] Changing Sample Numerator 5.8

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5.3 ABBREVIATIONS

A, ABS ................................ Absorbance

ARB ...................................... Absorbance of reagent blank
ARB,0 .................................... At Fixed Time: Absorbance of reagent blank after incubation time T0
ARB,1 .................................... At Fixed Time: Absorbance of reagent blank after reaction time T1
ARBB ..................................... Absorbance of blank of reagent blank
AS ........................................ Absorbance of sample
AS,0 ...................................... At Fixed Time: Absorbance of sample after incubation time T0
AS,1 ...................................... At Fixed Time: Absorbance of sample after reaction time T1
AS,0 ... AS,5 ........................... At Kinetic: Initial absorbance and 1st ... 5th absorbance of sample
ASB ...................................... Absorbance of sample blank
AST....................................... Absorbance of standard
AST,0..................................... At Fixed Time: Absorbance of standard after incubation time T0
AST,1..................................... At Fixed Time: Absorbance of standard after reaction time T1
ASTB ..................................... Absorbance of standard blank

C ......................................... Concentration
CST ...................................... Concentration of standard

∆ARB,Minute ............................ At Kinetic: Change of reagent blank per minute (measured in ∆A / min)
∆AS,Minute .............................. At Kinetic: Change of sample per minute (measured in ∆A / min)

F.......................................... Factor
FTK ..................................... Fixed Time Kinetic

KIN ...................................... Kinetic

nm ....................................... Nanometer (dimension of wavelength)

R ......................................... Result, Sample

Rb ....................................... Reagent blank
Rbb ..................................... Blank of Reagent blank
R^2...................................... At Kinetic: Square of correlation coefficient shows the linearity of a test

S, ST ................................... Standard
STb ..................................... Standard blank

Sb........................................ Sample blank

TRANSM., T........................ Transmission in %

T0......................................... At Fixed Time: Incubation time in seconds
T1......................................... At Fixed Time: Reaction time in seconds
T1......................................... At Kinetic: Time per delta in seconds

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 5.4

The calculation procedures, on which all methods are traceable from the list of methods, are mentioned in the following table. Criterion is the characteristic
of the calculation procedure (see below). For detailed description of the respectively accompanying course of method see chapter 5.5 - DESCRIPTION OF METHOD
4040e_23.doc / 05.04.07

COURSES

CP-No. *) Description*) Characteristic*) Method Calculation formula*) Factor RB

CP 1 chapter 5.5.1 C/F End Point with Factor C = F ∗ AS given / entering --
CP 2 chapter 5.5.2 C/F/Rb End Point with Factor C = F ∗ (AS - ARB) given / entering ent./meas.

ROBERT RIELE GmbH & Co KG

CP 3 chapter 5.5.3 C/F/Sb End Point with Factor C = F ∗ |AS - ASB| given / entering --
CP 4 chapter 5.5.4 C/F/SbRb End Point with Factor C = F ∗ (|AS - ASB| - |ARB - ARBB|) given / entering ent./meas.
SURVEY OF THE METHODS

CP 5 chapter 5.5.5 C/S End Point with Standard C = F ∗ AS F = CST / AST --

CP 6 chapter 5.5.6 C/S/Rb End Point with Standard C = F ∗ (AS - ARB) F = CST / (AST-ARB) ent./meas.
CP 7 chapter 5.5.7 C/S/Sb End Point with Standard C = F ∗ |AS - ASB| F = CST / |AST-ASTB| --
CP 8 chapter 5.5.8 C/S/SbRb End Point with Standard C = F ∗ (|AS - ASB| - |ARB - ARBB|) F = CST / ( |AST-ASTB| - |ARB-ARBB| ) ent./meas.

Page 19
CP 9 chapter 5.5.9 FTK/F/Rb Fixed Time with Factor C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| ) given / entering ent./meas.
CP 10 chapter 5.5.10 FTK/S/Rb Fixed Time with Standard C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| ) F = CST / (|AST,0-AST,1|-|ARB,0-ARB,1|) ent./meas.
CP 11 chapter 5.5.11 KIN/F/Rb Kinetic with Factor C = F ∗ ( ∆AS,Minute - ∆ARB,Minute ) given / entering ent./meas.
CP 12 chapter 5.5.12 KIN/S/Rb Kinetic with Standard C = F ∗ ( ∆AS,Minute - ∆ARB,Minute ) F = CST / ( ∆AST,Minute - ∆ARB,Minute ) ent./meas.
CP 13 chapter 5.5.13 TRANSM. Transmission in %
CP 14 chapter 5.5.14 C/Fdelta Difference with Factor C = F ∗ ( ∆AS2-Sb2 - ∆AS1-Sb1 ) given / entering meas.
CP 15 chapter 5.5.15 C / F 3 WL Meas. with 3 wavelengths C [mg/dl]= 168 * A415nm – 84 * A380nm
– 84 * A450nm meas.

*) Explanations:
CP-No. ................................................. Number of the calculation procedure (see also chapter 7 - METHOD EDITOR)
Description ........................................... Refer to the description of the corresponding method course
Characteristic ....................................... Characteristic of the calculation procedure (see also chapter 7 - METHOD EDITOR and chapter 13 - METHOD LIST)
Calculation formula .............................. Calculation basis of basic method, abbreviations and formula sign (chapter 5.3 - ABBREVIATIONS)
Rb ........................................................ Reagent blank
ent./meas.. ........................................... Reagent blank can be entered or measured

5 - MEASUREMENT WITH BASIC METHODS

4040e_23.doc / 05.04.07

5.5 DESCRIPTION OF METHOD COURSES

At first the device runs through preparatory measuring till a sequence starts which recurs for every further test.

5.5.1 Concentration Measurement with Factor

Method at which a measured sample value AS is multiplied with a predefined factor F.

Calculation procedure .......................................................CP 1

Characteristic ....................................................................C / F
Method ....................................................End Point with Factor
Calculation formula .................................................. C = F ∗ AS
Factor............................................................... given / entering

Course representation (preprogramable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of factor pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

5.5.2 Concentration Measurement with Factor and Reagent Blank

Method at which the difference of sample value AS and reagent blank ARB is multiplied with a given factor F.
The reagent blank ARB is entered or measured once.

Calculation procedure .......................................................CP 2

Characteristic ............................................................ C / F / Rb
Method ....................................................End Point with Factor
Calculation formula .......................................C = F ∗ (AS - ARB)
Factor............................................................... given / entering
Reagent blank ........................................entering or measuring

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of factor pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
Bringing in and measuring reagent blank (Rb)
or: Input of reagent blank (Rb) in absorbance
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

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5.5.3 Concentration Measurement with Factor and Sample Blank

Method at which the difference of sample value AS and sample blank ASB regarding the amount is multiplied with
a given factor F. The sample blank ASB is measured before every test.

Calculation procedure .......................................................CP 3

Characteristic ............................................................ C / F / Sb
Method ....................................................End Point with Factor
Calculation formula ....................................... C = F ∗ |AS - ASB|
Factor............................................................... given / entering

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of factor pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample blank (Sb)
Measuring sample blank (Sb)
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

5.5.4 Concentration Measurement with Factor, Sample Blank and Reagent Blank
Method at which the difference of reagent blank ARB and blank of reagent blank ARBB regarding the amount is
subtracted of the difference of sample value AS and sample blank ASB regarding the amount, and this difference
is multiplied with a given factor F.
The sample blank ASB is measured before every test. The reagent blank ARB is entered or measured once.

Calculation procedure .......................................................CP 4

Characteristic ........................................................ C / F / SbRb
Method ....................................................End Point with Factor
Calculation formula ..............C = F ∗ ( |AS - ASB| - |ARB - ARBB| )
Factor............................................................... given / entering
Reagent blank ........................................entering or measuring

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of factor pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
Bringing in blank of reagent blank (Rbb)
Measuring blank of reagent blank (Rbb)
Bringing in reagent blank (Rb)
Measuring reagent blank (Rb)
or: Input of difference Rb-Rbb in absorbance
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample blank (Sb)
Measuring sample blank (Sb)
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

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5.5.5 Concentration Measurement with Standard

Method at which a measured absorbance value AS is multiplied with a factor F which is determined by
measuring of a standard solution with known concentration CST.

Calculation procedure .......................................................CP 5

Characteristic ....................................................................C / S
Method ............................................... End Point with Standard
Calculation formula .................................................. C = F ∗ AS
Resulting factor .................................................... F = CST / AST

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of standard concentration CST pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or break with [BREAK]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

5.5.6 Concentration Measurement with Standard and Reagent Blank

Method at which the difference of sample value AS and reagent blank ARB is multiplied with a factor F which is
determined by measuring of a standard solution with known concentration CST and under consideration of
reagent blank ARB.
The reagent blank ARB is entered or measured once.

Calculation procedure .......................................................CP 6

Characteristic ............................................................ C / S / Rb
Method ............................................... End Point with Standard
Calculation formula .......................................C = F ∗ (AS - ARB)
Resulting factor ........................................... F = CST / (AST-ARB)
Reagent blank ........................................entering or measuring

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of standard concentration CST pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
Bringing in and measuring reagent blank (Rb) or: Input of reagent blank (Rb) in absorbance
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or break with [BREAK]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

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5.5.7 Concentration Measurement with Standard and Sample Blank

Method at which the difference of sample value AS and sample blank ASB regarding the amount is multiplied with
a factor F which is determined by measuring of a standard solution with known concentration CST and under
consideration of standard blank ASTB.
The sample blank ASB is measured before every test.

Calculation procedure .......................................................CP 7

Characteristic ............................................................ C / S / Sb
Method ............................................... End Point with Standard
Calculation formula ....................................... C = F ∗ |AS - ASB|
Resulting factor ........................................ F = CST / |AST - ASTB|

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of standard concentration CST pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
Bringing in standard blank (STb)
Measuring standard blank (STb)
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or break with [BREAK]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample blank (Sb)
Measuring sample blank (Sb)
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

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5.5.8 Concentration Measurement with Standard, Sample Blank and Reagent Blank
Method at which the difference of reagent blank ARB and blank of reagent blank ARBB regarding the amount is
subtracted of the difference of sample value AS and sample blank ASB regarding the amount and this difference
is multiplied with a factor F which is determined by measuring of a standard solution with known concentration
CST and under consideration of standard blank ASTB and the difference of reagent blank ARB and blank of
reagent blank ARBB
The sample blank ASB is measured before every test. The reagent blank ARB is entered or measured once.

Calculation procedure .......................................................CP 8

Characteristic ........................................................C / S / SbRb
Method ............................................... End Point with Standard
Calculation formula ..............C = F ∗ ( |AS - ASB| - |ARB - ARBB| )
Resulting factor ...................F = CST / ( |AST-ASTB| - |ARB-ARBB| )
Reagent blank ........................................entering or measuring

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Input of standard concentration CST pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
Bringing in blank of reagent blank (Rbb)
Measuring blank of reagent blank (Rbb)
Bringing in reagent blank (Rb)
Measuring reagent blank (Rb)
or: Input of difference Rb-Rbb in absorbance
Bringing in standard blank (STb)
Measuring standard blank (STb)
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or break with [BREAK]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample blank (Sb)
Measuring sample blank (Sb)
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

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5.5.9 Fixed Time with Factor

Method at which is measured a reagent blank after an incubation time (⇒ ARB,0) and after a reaction time (⇒
ARB,1) and also a sample after an incubation time (⇒ AS,0) and after a reaction time (⇒ AS,1).
The difference from the change of the test and the change of the reagent blank is multiplied by a predefined
factor F. The reagent blank ARB is entered or measured once.

While performing the course the dialog asks for the use of a reagent blank. The default value is OFF. To
continue without reagent blank press [ENTER].

Calculation procedure .......................................................CP 9

Characteristic ........................................................ FTK / F / Rb
Method ................................................. Fixed Time with Factor
Calculation formula ......... C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )
Factor............................................................... given / entering
Reagent blank ........................................entering or measuring

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Input of factor pre-programmable
Temperature ON/OFF pre-programmable
Input of incubation time pre-programmable
Input of reaction time pre-programmable
Bringing in and measuring zero solution
Bringing in and measuring reagent blank (Rb)
or: Input of difference of reagent blanks |ARB,0-ARB,1| in absorbance
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
• Confirming result and changing to next sample with [ENTER]
• or: Further measuring of AS,1 with [RESULT]
• or: Bringing in zero solution and measuring zero with [ZERO]
• or: Break with [BREAK]
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

Abbreviations at printout:
Rb[A] Initial value of Rb in absorbance
DELTA ABS Difference between both measurements in absorbance
Column „ABS.“ Initial value of Kinetic in absorbance
Column „RESULT“ Result of Kinetic under consideration of Rb

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5.5.10 Fixed Time with Standard

Method at which is measured a reagent blank after an incubation time (⇒ ARB,0) and after a reaction time (⇒
ARB,1) and also a sample after an incubation time (⇒ AS,0) and after a reaction time (⇒ AS,1).
The difference from the change of the sample and the change of the reagent blank becomes multiplied with a
factor F which is determined by means of the change of standard solution |AST,0-AST,1| and the change of
reagent blank |ARB,0-ARB,1| during the reaction time and given concentration of standard. The reagent blank ARB
is entered or measured once.

While performing the course the dialog asks for the use of a reagent blank. The default value is OFF. To
continue without reagent blank press [ENTER].

Calculation procedure .....................................................CP 10

Characteristic ........................................................ FTK / S / Rb
Method ............................................. Fixed Time with Standard
Calculation formula ......... C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )
Resulting factor .............. F = CST / ( |AST,0-AST,1| - |ARB,0-ARB,1| )
Reagent blank ........................................entering or measuring

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Input of standard concentration CST pre-programmable
Temperature ON/OFF pre-programmable
Input of incubation time pre-programmable
Input of reaction time pre-programmable
Bringing in and measuring zero solution
Bringing in and measuring reagent blank (Rb)
or: Input of difference of the reagent blanks |ARB,0-ARB,1| in absorbance
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or break with [BREAK]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
• Confirming result and changing to next sample with [ENTER]
• or: Further measuring of AS,1 with [RESULT]
• or: Bringing in zero solution and measuring zero with [ZERO]
• or: Break with [BREAK]
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

Abbreviations at printout:
Rb[A] Initial value of Rb in absorbance
DELTA ABS Difference between both measurements in absorbance
ST[A] Initial value of standard in absorbance
Column „ABS.“ Initial value of Kinetic in absorbance
Column „RESULT“ Result of Kinetic under consideration of Rb

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5.5.11 Kinetic with Factor

Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute ∆AS,Minute is determined by a regression
calculation. The reagent blank ∆ARB,Minute is measured in the same way as the sample (or entered directly in U/l)
and subtracted from the sample value. This difference is multiplied by a given factor F.

While performing the course the dialog asks for the use of a reagent blank. The default value is OFF. To
continue without reagent blank press [ENTER].

Calculation procedure .....................................................CP 11

Characteristic .........................................................KIN / F / Rb
Method ........................................................ Kinetic with Factor
Calculation formula .................C = F ∗ ( ∆AS,Minute - ∆ARB,Minute )
Factor............................................................... given / entering
Reagent blank ........................................entering or measuring
Number of deltas........................................... entering (3 to 28)
Time per delta ........................................entering (4 s to 255 s)

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Input of factor pre-programmable
Temperature ON/OFF pre-programmable
Input of delay before 1st measuring pre-programmable
Input of number of deltas pre-programmable
Input of time per delta pre-programmable
Bringing in and measuring zero solution
Bringing in reagent blank (Rb)
Measuring reagent blank (Rb)
Output of result of reagent blank in U/l and of correlation coefficient R^2 - confirming with [ENTER]
or: Input of reagent blank (Rb) in U/l and confirming with [ENTER]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
• Confirming result and changing to next sample with [ENTER]
optional with [+] detailed print-out of U/l in each measuring interval
• or: Measuring same sample with [RESULT]
• or: Bringing in zero solution and measuring zero with [ZERO]
• or: Break with [BREAK]
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

Abbreviations at printout:
Rb[A] Initial value of Rb in absorbance
DELTA ABS Result in ∆A / min, generated from the first up to the last measurement in absorbance
DELTA REG Result in ∆A / min, generated from a regression calculation
R^2 Square of correlation coefficient shows the linearity of the test
Column „ABS.“ Initial value of Kinetic in absorbance
Column „RESULT“ Result of Kinetic under consideration of Rb

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5.5.12 Kinetic with Standard

Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute ∆AS,Minute is determined by a regression
calculation. The reagent blank ∆ARB,Minute is measured in the same way as the sample (or entered directly in U/l)
and subtracted from the sample value. This difference is multiplied by a factor F which is determined by
measuring of a standard solution ∆AST,Minute with known concentration CST and under consideration of the
reagent blank ∆ARB,Minute.

While performing the course the dialog asks for the use of a reagent blank. The default value is OFF. To
continue without reagent blank press [ENTER].

Calculation procedure .....................................................CP 12

Characteristic .........................................................KIN / S / Rb
Method .................................................... Kinetic with Standard
Calculation formula .................C = F ∗ ( ∆AS,Minute - ∆ARB,Minute )
Resulting factor ................... F = CST / ( ∆AST,Minute - ∆ARB,Minute )
Reagent blank ........................................entering or measuring
Number of deltas........................................... entering (3 to 28)
Time per delta ........................................entering (4 s to 255 s)

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Input of standard concentration CST pre-programmable
Temperature ON/OFF pre-programmable
Input of delay before 1st measuring pre-programmable
Input of number of deltas pre-programmable
Input of time per delta pre-programmable
Bringing in and measuring zero solution
Bringing in reagent blank (Rb)
Measuring reagent blank (Rb)
Output of result of reagent blank ∆ARB,Minute in U/l and of correlation coefficient R^2 - confirming with [ENTER]
or: Input of reagent blank (Rb) in U/l and confirming with [ENTER]
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or break with [BREAK]
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
• Confirming result and changing to next sample with [ENTER]
optional with [+] detailed print-out of U/l in each measuring interval
• or: Measuring same sample with [RESULT]
• or: Bringing in zero solution and measuring zero with [ZERO]
• or: Break with [BREAK]
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

Abbreviations at printout:
Rb[A] Initial value of Rb in absorbance
DELTA ABS Result in ∆A / min, generated from the first up to the last measurement in absorbance
DELTA REG Result in ∆A / min, generated from a regression calculation
R^2 Square of correlation coefficient shows the linearity of the test
ST/KIN 1 … ST/KIN 3 Result of standard 1 ... 3 in ∆A / min
Column „ABS.“ Initial value of Kinetic in absorbance
Column „RESULT“ Result of Kinetic under consideration of Rb

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5.5.13 Transmission Measurement

Calculation procedure .....................................................CP 13

Characteristic ................................................................. T in %

Course representation (pre-programmable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Bichromatic pre-programmable
Temperature ON/OFF pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------

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5.5.14 Difference with Factor

Method at which a difference of sample E2 – E1 is measured several times depending on the quantity of
samples. In the first course the sample E1 (maximum 25) will be measured, optionally with or without sample
blank. After a user defined time the sample E2 will be measured in a second course. Attention should be paid to
the order within the series to avoid errors. The procedure corresponds to a fixed time kinetic and is normally
processed using the standard cuvette adaptor. At the beginning of the method the use of a sample blank is
queried.
Quality control samples can not be saved.

Calculation procedure .....................................................CP 14

Characteristic ........................................................ C / F / Delta
Method ...................................................Difference with Factor
Calculation formula ..................... C = F ∗ ( ∆AS2-Sb2 - ∆AS1-Sb1 )
Factor............................................................... given / entering
Sample blank ....................................................... with / without
Number of deltas........................................measuring (1 to 25)
Time per delta ................................................... manual control

Start method selection in the main menu.

PHOTOMETER 4040 #1000 See chapter:
V2.3a 03.04.07 D 4 - SELECTION OF MEASURING PROGRAMS
LAB.: RIELE GMBH+CO KG
DATE: 03.04.07
TIME: 07:16:31
METHOD 14: C/Fdelta
PROGRAM: 14 In the case of activated printer the print out of the method data
FACTOR: 1.000 follows.
WAVELENGTH: 405nm
TEMPERATURE: 37C
DELAY: 2s The measuring window is shown.
UNIT: U/l
- - - - - - - - - - - -
MEASURE BLANK Method procedure with sample blank:

NO. Sb[A] S[A]E1 ÆInsert / measure zero solution

1 0.083 0.411
2 0.110 0.382
3 0.146 0.492 ÆMeasure all samples E1 (maximum 25)

NO. Sb[A] S[A]E2 ÆChange to measuring E2 by pressing [+]

1 0.091 1.090
2 0.140 0.991
3 0.200 1.165 ÆMeasure all samples E2 (maximum 25)

NO. RESULT ÆFinish the measuring by pressing [+]

1 0.671
2 0.578 Results based on the differences of the measured samples
3 0.619
- - - - - - - - - - - - ÆShow the results by pressing [+]

NO. Sb[A] S[A]E1 Method procedure without sample blank:

1 0.000 1.012
2 0.000 1.138 ÆMeasure all samples E1 (maximum 25)
3 0.000 1.076
ÆChange to measuring E2 by pressing [+]
NO. Sb[A] S[A]E2
1 0.000 1.458
ÆMeasure all samples E2 (maximum 25)
2 0.000 1.530
3 0.000 1.384
ÆFinish the measuring by pressing [+]
NO. RESULT
Results based on the differences of the measured samples
1 0.446
2 0.392
ÆShow the results by pressing [+]
3 0.307

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5.5.15 Measurment with 3 wavelengths

Method at which a sample is measured with three different wavelengths: 380 nm, 415 nm and 450 nm. This
method is appropiated for free hemoglobin measurements.
The mentioned wavelengths are not included in the standard set of filters.

Calculation procedure ..................................................................................... CP 15

Characteristic ............................................................................................ C / F 3 WL
Method .................................................................... Measurment with 3 wavelengths
Calculation formula ...................... C [mg/dl]= 168 * A415nm – 84 * A380nm – 84 * A450nm
Unit conversion formula .................................................... mmol/L ≡ 0,6206 * mg / dl

Start method selection in the main menu.

PHOTOMETER 4040 #1000 See chapter:
V2.3a 03.04.07 D 4 - SELECTION OF MEASURING PROGRAMS
LAB.: RIELE GMBH+CO KG
DATE: 03.04.07
TIME: 07:16:31
METHOD 15: C/F 3WL
PROGRAM: 15 In the case of activated printer the print out of the method data
WAVELENGTH: follows.
380/415/450nm
TEMPERATURE: 37C The measuring window is shown.
DELAY: 2s
- - - - - - - - - - - -
MEASURE BLANK

NO. mmol/L mg/dl ÆInsert / measure zero solution

1 4.859 7.83
- - - - - - - - - - - - ÆThe result will be displayed in two units of measurement
2 5.865 9.45
ÆInsert / measure sample

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5.6 Printer ON / OFF

Start menu by [MODE] [PARALLEL]:

The internal printer can be activated or deactivated.

PRINTER ON The present status of the printer is displayed in the first line.
1/ON 0/OFF Activate printer by [1] or deactivate by [0].
With [BREAK] the setting is taken temporarily up to next switching on.
With [ENTER] the status is stored.

5.7 Serial interface utilities

At the rear, the Photometer 4040 has an RS 232 serial interface through which it can be connected to a PC or
an external printer. A suitable data cable can be supplied (REF 501-002). The connected device must comply
with safety standard EN 60950.

Start menu by [MODE] [SERIAL]:

The interface for serial inputs / outputs can be activated or deactivated

MENU SER. 1-5/+ • Item [1]: Activate or deactivate external printer with serial interface by [1]
• Item [2]: Activate or deactivate EDP by [2]
• Item [3]: Activate remote control by [3]
• Item [4]: Select baud rate of serial interface by [4]
• Item [5]: Download of operating system to FLASH MEMORY by [5]

Select item.

Item 1: External printer with serial interface

S PRINTER OFF The present status of the printer is displayed in the first line.
1/ON 0/OFF Activate printer by [1] or deactivate by [0].

Confirm with [ENTER].

Item 2: EDP
EDP OFF The present status of the EDP interface is displayed in the first line.
1/ON 0/OFF Activate EDP by [1] or deactivate by [0].

Confirm with [ENTER].

EDP CONFIG. 1 In case of activation:

0/S-E-B 1/CR-LF The present status of the EDP protocol is displayed in the first line.
Select STX / ETX / BCC protocol by [0] or select CR / LF protocol by [1].

Confirm with [ENTER].

Item 3: Remote control

REMOTE Confirm with [ENTER].
ON The keyboard is turned off. The control of the device has to be done by e.g. a
computer.

Normally the remote control mode can be stopped by [BREAK].

With a suitable program the Photometer 4040 can be remote controlled by a PC,
connected by a 1:1 data cable (REF 501-002) to the RS-232 serial interface.

Item 4: Baud rate

The present status of the baud rate is displayed in the first line.
BAUDRATE 9600 Select 4800 bps by [0] or select 9600 bps by [1].
0/4800 1/9600
Confirm with [ENTER].

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Item 5: Download to FLASH MEMORY

The FLASH MEMORY is a non-volatile memory which may be erased and
DOWNLOAD programmed electrically. This feature allows replacing the operating system of
INPUT +/E/B Photometer 4040.
The process is done with utility DOWNLOAD and a software module running on a
personal computer. Both devices, Photometer 4040 and the personal computer,
have to be connected by a serial interface cable.

Confirm with [ENTER].

For further information see SERVICE MANUAL chapter 6.3 – Download to

FLASH MEMORY.

5.8 Changing Sample Numerator

- Possible only within a course of method with [MODE] [NUM.] -

The sample numbering can start with any number between 0 and 99. The
modification of numbering can be carried out also during a sequence of
measurement. The new numbering gets effective after triggering of a measuring.

SAMPLE NO. Enter next number and confirm with [ENTER].

1

5.9 Multi-standard tools

Linear calibration is used in the case of two calibrators. The assay absorbance at different concentrations forms
a linear plot.
Nonlinear calibration is used for those sample constituents where assay absorbance at different concentrations
forms a nonlinear but reproducible plot. At least three (maximum 20) calibrators are required for nonlinear
calibration.

A multi-standard method - built up on the basic methods - can be created and stored with the method editor
(chapter 7 - METHOD EDITOR). The prototype of the method (basic method) has to be a calculation procedure
based on endpoint with factor (CP 1, 2, 3 and 4), fixed time kinetic with factor (CP 9) or kinetic with factor
(CP 11). The new method has to be saved marked “MULTI STD” in the range of method number 20 to 249.

For the programmed method a calibration curve is assigned with the same number. Up to 50 nonlinear
calibration curves with maximal 20 sets of points (calibrators) can be stored.

A special mode function opens a submenu with four multi-standard tools (chapter 5.2 - MODE FUNCTIONS).

Start menu by [MODE] [5]:

MENU MULTI STD Item [1]: Measurement and storing of calibrators

1-4/+ Item [2]: Graphic print-out of curve
Item [3]: Edit given data points of a curve
Item [4]: Show all stored multi-standard methods

Select item.

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Item 1: Measurement and storing of calibrators

The measurement of the calibrators can be performed.
Start menu by [MODE] [5] [1].
MEASURE CURVE
NEXT KEY E/B/+ Confirm with [ENTER].

CURVE NO. 20 The number of the pre-selected calibration curve (e.g. 20) is displayed. Confirm
NEXT KEY E/B/# with [ENTER] or change the number according to another existing calibration
curve.

METHOD NO. The main menu is displayed. The system is prepared in a special mode for
creating the selected calibration curve.
INPUT 1-250
With the following basic methods using standards the calibrators can be
measured. The special mode is again switched off by selection of a method not
listed.

• 5.5.5 - Concentration Measurement with Standard

• 5.5.6 - Concentration Measurement with Standard and Reagent Blank
• 5.5.7 - Concentration Measurement with Standard and Sample Blank
• 5.5.8 - Concentration Measurement with Standard, Sample Blank and
Reagent Blank
• 5.5.10 - Fixed Time with Standard
• 5.5.12 - Kinetic with Standard

M5 Call e.g. method 5 (Concentration Measurement with Standard).

Enter wavelength, given concentration of calibrator, temperature control and
C/S
delay.

Insert blank.
Press [ZERO].

Now the Photometer 4040 is ready to measure all calibrators one after the other.
Insert the first calibrator. Press [RESULT].

0.301 0 The first absorbance (e.g. 0.301) of the calibrator is shown.

The cursor marks the second value of the absorbance (e.g. 0).
0 E/+/0
With [+] skip to the next value.
With [0] the selected value is deleted.
In case of double determination: With [RESULT] the next reading of the calibrator
is performed.
In case of triple determination: With [RESULT] the next reading of the calibrator is
performed.

With [ENTER] the value is accepted.

M: 20 A: 0.301 In the first line the number of the calibration curve (e.g. M: 20) and the
P: 1 C: 60 absorbance of the calibrator (e.g. A: 0.301) is displayed.
In the second line the number of the data point (e.g. P: 1) and the concentration
of the calibrator (e.g. C: 60) ) is displayed.
The number of the data point can be edited between 1 and 20.

NEW STANDARD With [ENTER] the value is stored as actual data point. On parallel a print-out with
all data is created (see below: Print-out of calibration procedure).
#
The next calibrator can be measured and stored as data point.

After the last measurement and storing of calibrator the procedure can be finished
by [BREAK], [BREAK].

CURVE STORE The calibration curve is stored with [1] or canceled with [0].
0/NO 1/YES In case of storing the set of data points will be sorted by ascending absorbance.

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Print-out of calibration procedure

PHOTOMETER 4040 # 1000

DATE: 03.04.07
TIME: 17:23:19
METHOD 5: C/S
PROGRAM: 5
STANDARD: 60
WAVELENGTH: 405nm
TEMPERATURE: OFF
DELAY: 0s
UNIT:

CURVE: 20

STANDARD: 60
MEASURE BLANK
ST[A] 1: 0.302
ST[A] 2: 0.304
ST[A] 3: 0.301
ST[A] : 0.302
DATA POINT: 1
STANDARD: 30
ST[A] 1: 0.601
ST[A] 2: 0.602
ST[A] 3: 0.599
ST[A] : 0.600
DATA POINT: 2
STANDARD: 15
ST[A] 1: 0.920
ST[A] 2: 0.922
ST[A] 3: 0.919
ST[A] : 0.920
DATA POINT: 3
STANDARD: 7.5
ST[A] 1: 1.160
ST[A] 2: 1.162
ST[A] 3: 1.157
ST[A] : 1.160
DATA POINT: 4
STANDARD: 3.25
ST[A] 1: 1.501
ST[A] 2: 1.499
ST[A] 3: 1.504
ST[A] : 1.501
DATA POINT: 5

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Item 2: Graphic print-out of curve

A curve and the given data points can be printed.
Start menu by [MODE] [5] [2].
PRINT CURVE
NEXT KEY E/B/+ Confirm with [ENTER].

CURVE NO. 20 The number of the pre-selected calibration curve (e.g. 20) is displayed. Confirm
NEXT KEY E/B/# with [ENTER] or change the contents equivalent to another existing calibration
curve.

Plot of nonlinear curve

The given data points are marked with dots.

Table of given data points P1 up to P5

Item 3: Edit given data points of a curve

The procedure allows editing of an existing curve.
Start menu by [MODE] [5] [3].
EDIT CURVE
NEXT KEY E/B/+ Confirm with [ENTER].

CURVE NO. 20 Select curve by numeric key.

NEXT KEY E/B/#

A 0.302 P 1 Continue with editing described in

C 60 EZB+- chapter 7 - METHOD EDITOR: Editing of standards

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Item 4: Show all stored multi-standard methods

The procedure shows the existing curves and their assigned methods.
Start menu by [MODE] [5] [4].
PARAM.METHODS
NEXT KEY E/B/+ Confirm with [ENTER].

M20 05.29.02 5 Displayed is e.g. the existing curve 20, created on 05.29.02, based on five given
NEXT KEY E/B data points.

Confirmation with [ENTER] skips to the next curve or finishes the procedure.

Example of print-out for method 20

PHOTOMETER 4040
DATE: 11.10.04
TIME: 17:34:25
METHOD 20: DEMO
PROGRAM: 1
FACTOR: 1.000
WAVELENGTH: 405nm
TEMPERATURE: OFF
DELAY: 1s
UNIT: ng/dl
MULTI STD

MEASURE BLANK

NO. ABS. RESULT

Result with a concentration of e.g. 24.177 ng/dl.
1 0.700 24.177
The absorbance has a value of 0.700 A.
2 1.001 12.107

3 1.303 5.526
Result with a concentration out of range. 4 1.570 <<<>>>

5 0.308 59.340
Result with a concentration out of range. 6 0.292 <<<>>>

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5.10 Patient data management

Up to 2970 results of patients can be managed in the memory of Photometer 4040. The results are stored
together with a two-digit patient number. The range of the patient numbers is starting with 1 and ending with 99.
The memory is available after switching off the instrument and dependent on the lifetime of the battery.
The memory is not a FIFO. Therefore no results can be lost. If the memory is filled with 2970 results, an addition
of further results is not able. The memory has to be emptied first by deleting results assigned to patient
numbers. In practice, the operator is asked for deleting the results after each print-out of patient data.
Up to 2970 results can be assigned to one patient number. On average 30 results can be stored in case of
working with 99 patient numbers.

5.10.1 Working with patients data within a course

The following procedure shows an example of storing results of a patient within a course.
Once the patients mode has to be activated by [MODE] [3] [1] (see next chapter).

86 12.5 mg/dl Within a course the two digit numerator of results is used as patient number.
Before measuring a sample enter patient number by using the MODE-function
MEASURE SAMPLE
[MODE] [NUM] (see chapter 5.8 - Changing Sample Numerator).

SAMPLE NO. Last patient number was e.g. 86. Confirm this number with [ENTER],
86 23 or enter current patient number, e.g. 23, and confirm with [ENTER].

86 12.5 mg/dl Measure next sample by [RESULT].

MEASURE SAMPLE

23 12.9 mg/dl Store result by [1] or skip over without storing by [0].
STORE ? 0/N 1/Y

5.10.2 Patient data management utilities

Start menu by [MODE] [3]:

MENU PAT. 1-4/+ Item [1]: Activate or deactivate patient mode

Item [2]: Print-out of patient related results *
Item [3]: Delete patients data *
Item [4]: Print-out of memory occupancy *
* Patient mode must be activated.

Select item.

Item 1: Activate or deactivate patient mode

Start menu by [MODE] [3] [1].

PATIENT ON/OFF Confirm with [ENTER].

NEXT KEY E/B/+

P-MODE OFF The present status of the patient mode is displayed in the first line.
1/ON 0/OFF Activate patient mode by [1] or deactivate by [0].
In case of a deactivated patients mode no more items are shown in the menu.
No results can be lost by deactivating patient mode.
Confirm with [ENTER].

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Item 2: Print-out of patient related results

A survey of all stored results is printed for a patient number.

Start menu by [MODE] [3] [2].

PRINT PATIENT Confirm with [ENTER].

NEXT KEY E/B/+

PATIENT NO. The last selected patient number is shown in the second line (e.g. 6).
Enter desired patient number (e.g. 20).
6 20
Confirm with [ENTER].
The print-out of results is started:

**** PATIENT REPORT ****

PHOTOMETER 4040 #1000
DATE: 11.10.04
LAB.: Labor xxxxx

PATIENT NO.: 20

DATE: 12.11.02
METHOD 55: tBIL.dcp
100 mg/dl
DATE: 12.12.02
METHOD 55: tBIL.dcp
104 mg/dl
DATE: 12.14.02
METHOD 55: tBIL.dcp
102 mg/dl
DATE: 12.15.02
METHOD 73: CAmtb
5.4 mg/dl
DATE: 12.16.02
METHOD 55: tBIL.dcp
101 mg/dl
DATE: 12.17.02
METHOD 73: CAmtb
5.1 mg/dl

DELETE PATIENT With [0] quit without deleting.

0/NO 1/YES With [1] quit with deleting printed data.

Confirm with [ENTER].

At select [1]:
ARE YOU SURE ? With [0] quit without deleting.
0/NO 1/YES With [1] quit with deleting.

Confirm with [ENTER].

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Item 3: Delete patients data

Start menu by [MODE] [3] [3].

DELETE PATIENT Confirm with [ENTER].

NEXT KEY E/B/+

PATIENT NO. The last selected patient number is shown in the second line (e.g. 6).
Input desired patient number (e.g. 20).
6 20
Confirm with [ENTER].

ARE YOU SURE ? With [0] quit without deleting.

0/NO 1/YES With [1] quit with deleting all results concerning selected patient number.

Confirm with [ENTER].

Item 4: Print-out of memory occupancy

A survey of all stored patient numbers with the number of results is printed.

Start menu by [MODE] [3] [4].

PRINT P-MEM.INF Confirm with [ENTER].

NEXT KEY E/B/+
The print-out shows maximum three sets of values at a line. A set of values
consists of the patient number and the number of stored results separated by a
slash. For example 5/ 4 means: 4 results of patient number 5 are stored in the
memory.

**** PATIENT MEMORY ****

PHOTOMETER 4040 #1000
DATE: 11.10.04

5/ 4 20/ 12 75/ 3
17/ 14 89/ 1 46/ 27

5.11 Lamp protection

LAMP OFF By [MODE] [1] the halogen lamp can be switched off temporarily to extend the
lifetime.

Activate the lamp protection by [ENTER].

LAMP OFF Deactivate the lamp protection by any key. After 60 s Photometer 4040 is again
59s ready for measuring.

A zero adjustment should be repeated by bringing in a zero solution and

pressing [ZERO].

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6 USER GUIDANCE AT DISPLAY

DISPLAY DESCRIPTION

Method selection:
METHOD NO. See chapter 4 - SELECTION OF MEASURING PROGRAMS
INPUT 1-250

Method selection:
M1 The method M1 – C/F is selected.
C/F See chapter 4 - SELECTION OF MEASURING PROGRAMS

Filter selection:
M1 FILTER Select actual wavelength by [ENTER].
F1 340NM +-# Change wavelength by [+], [-] or numeric key [#].

Factor, standard, etc.:

M1 FACTOR Enter the value by numeric key [#].
#

Temperature selection:
M1 TEMPERATURE Switch temperature ON or OFF by [+], [-] or numeric key [#].
0 OFF +-#

Delay selection:
M1 DELAY Enter the value in seconds by numeric key [#].
s #

Supply and setting to zero of zero solution by [ZERO].

M1 Then setting to zero is displayed by a 0 in the first line and printed out.
MEASURE BLANK Setting to zero can be repeated within a series of measuring.

Supply and measurement of sample solution.

0 Z[A]
MEASURE SAMPLE

In the first line the result 61 of sample no. 1 is shown.

1 61 R[ ] Supply and measurement of sample solution.
MEASURE SAMPLE

In the first line the result 61 mg/dl of sample no. 1 is shown.

1 61 mg/dl Supply and measurement of sample.
MEASURE SAMPLE

Supply and measurement of reagent blank solution.

0 Z[A] Instead of being measured, the reagent blank can also be entered via
MEASURE Rb the keyboard or put on zero.

Supply and measurement of blank of reagent blank solution.

0 Z[A]
MEASURE Rbb

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DISPLAY DESCRIPTION

Supply and measurement of sample blank.

0 Z[A]
MEASURE Sb

Enter given concentration value of standard including decimal point.

M5 STANDARD
#

The last determined resulting factor of a standard measuring is saved

OLD STD 415 together with the corresponding method no. After renewed selection of
0/NO 1/YES the same method no. this resulting factor is offered as „Old STD“. In all
other cases the data of the standard get rejected.

Supply and measurement of standard solution.

0 Z[A]
MEASURE ST

Enter incubation time in seconds.

M9 INCUBATION
s #

Enter reaction time in seconds.

M9 REACTION
s #

With [ENTER] the result is accepted. The next measurement can be

1 0.243 R[ ] started.
NEXT KEY E/R/Z/B With [RESULT] a new measurement replaces the old one without
touching the numerator.
With [ZERO] a zero adjustment is made.
With [BREAK] the method is finished.
Enter number of deltas of a kinetic method.
M11 DELTAS
#

Enter time per delta of a kinetic method.

M11 TIME/DELTA
s #

While performing the course the dialog asks for the use of a reagent
RB OFF blank.
1/ON 0/OFF The default value is OFF.
To continue without reagent blank press [ENTER].
Sample no. 1:
1 0.701 R[ ] Initial value of absorbance: 0.701
DELTA 1 5d1

Sample no. 1:
1 -0.021 /MIN. Delta 3 in absorbance / minute: -0.021
DELTA 3 5d1

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7 METHOD EDITOR
Based on the 15 calculation procedures, up to 230 user-defined methods can be created and saved.
Break of method editor and change back to program selection with [BREAK] at any time. So the program can be
used like a viewer.
Every input has to be confirmed with [ENTER].
Changing of attributes of the fixed defined methods in the range of 1 up to 19 is not possible.

Start menu with [MODE] [EDIT]:

METHOD EDIT Confirm with [ENTER].

NEXT KEY E/B

SOURCE 1 Enter the number of the method (e.g. 1 -> C/F, see chapter 13.1 - BASIC
INPUT 1-250 METHODS) which is to become the prototype (SOURCE) of the new method.
Confirm with [ENTER].

TARGET 20 Enter the number of the new method (TARGET e.g. 20).
INPUT 20-249 Confirm with [ENTER].
A corresponding error message (ERROR 16) is carried out if the space of method
memory is already occupied. When required, the method in question can be
erased (Utility program 8.2.4 - Deleting method) and the space of method
memory can be occupied newly.
In the range of 20 up to 249 an existing method can be changed by using the
same number for the prototype method as for the new method.

COPY ? • Item [1]: The new method will be created as method 20.
0/NO 1/YES Method 20 is identical with method 1. The operation will be
finished.

• Item [0]: The procedure allows constructing the new method with different
attributes. The queue of attributes is dependent on the attributes of
the prototype. Generally default inputs can be confirmed by
[ENTER] or replaced by new inputs.

Select item.

Item 0:

PROG. C/F 1 Input of name of new method ( e.g. ABC ). The string is limited to eight
NAME ABC characters.

The number keys are each occupied four times (see TEXT EDITOR LEVEL).
The desired character is selected by repeated push of the corresponding key.

Key [7]: A...B...C...D Key [8]: E...F...G...H Key [9]: I...J...K...L

Key [4]: M...N...O...P Key [5]: Q...R...S...T Key [6]: U...V...W...X
Key [1]: Y...Z...1...2 Key [2]: 3...4...5...6 Key [3]: 7...8...9...0
Key [0]: /...#...%...-

With key [WASH / A ↔ a]: Change between capital and lower letters
(flashing cursor = lower letters)
With key [ • / Chr. →]: Change of the cursor to the next input position
With key [ENTER]: Accepting and ending the input
Correction of input: By change of the cursor beyond the last place
and new input as of the first place

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PROG. Input of unit of new method ( e.g. g/l ). The string is limited to six characters.
UNIT g/l The input is done by the same procedure like creating the name (see above).

PROG.FACTOR Input of factor of new method ( e.g. 123.4 ).

0 123.4 # In case of a method based on standard the first line is replaced by
PROG.STANDARD.

PROG.FILTER Input of wavelength of new method ( e.g. 340 nm ) by [+], [-] or numeric key [#].
F1 340NM +-#

PROG.TEMPERATURE Input of temperature control of new method ( e.g. 1 ).

1 37C +-# • Selection 0 means: Temperature control OFF
• Selection 1 means: Temperature control 37 °C

In case of calculation procedure end point (CP 1 up to CP 8, CP 13 and CP 14):

BICHROM. OFF Bichromatic: select item [1] for bichromatic measurement

1/ON 0/OFF or item [0] for not bichromatic measurement

1 / ON:

BI-NM Select the bichromatic wavelength with +, - or enter a number (0 to 9)

F0 DDDNM +-# See chapter 5.1.6 - Fundamental to bichromatic measurements …

PROG.DELAY Input of delay before every measuring ( e.g. 5 s ).

0s 5s +-# The query of the delay can be suppressed by entering [65535].

PROG.MIN.UNITS Enter lower limit of a measurement result 5-digit + comma + sign.

0 # A zero deactivates this option.
If the measured value falls below the entered one, the error message "ERROR
MIN. UNITS" is shown.

PROG.MAX.UNITS Enter upper limit of a measurement result 5-digit + comma + sign.

0 # A zero deactivates this option.
If the measured value exceeds the entered one, the error message "ERROR
MAX.UNITS" is shown.

In case of calculation procedure fixed time (CP 9 and CP 10):

PROG.INCUBATION Input of incubation time before the first measuring ( e.g. 30 s ).

0s 30s # The query of the delay can be suppressed by entering [65535].

PROG.REACTION Input of reaction time before the first measuring ( e.g. 60 s ).

0s 60s # The query of the delay can be suppressed by entering [65535].

PROG.MIN.UNITS Enter lower limit of a measurement result 5-digit + comma + sign.

0 # A zero deactivates this option.
If the measured value falls below the entered one, the error message "ERROR
MIN. UNITS" is shown.

PROG.MAX.UNITS Enter upper limit of a measurement result 5-digit + comma + sign.

0 # A zero deactivates this option.
If the measured value exceeds the entered one, the error message "ERROR
MAX.UNITS" is shown.

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In case of calculation procedure kinetic (CP 11 and CP 12):

PROG.DELAY Input of delay before every measuring ( e.g. 5 s ).

0s 5s +-# The query of the delay can be suppressed by entering [65535].

PROG.DELTAS Input of deltas in the range of 3 up to 28 (e.g. 3).

0 3 #

PROG.TIME/DELTA Input of time per delta in the range of 4 up to 255 (e.g. 20).
0s 20 s #

PROG.MIN.R^2 Input of low limit of the correlation coefficient R^2 (e.g. 0.998) for linearity control.
0 0.998 # A zero deactivates this option.
If the measured value falls below the entered one, the error message
"ERROR R^2" is shown.

PROG.MAX.UNITS Enter upper limit of the measurement result in Units with right plus or minus sign
0 0 # (+ climbing / - falling Kinetic).
A zero deactivates this option.
If the measured value exceeds the entered one, the error message "ERROR
MAX.UNITS" is shown, at Kinetics additionally "ERROR+/-".

All other cases:

PROG.MULTI STD • Item [0]: The new method can only work with one factor or one standard.
0/NO 1/YES The method editor finishes his work.

• Item [1]: The new method works with several standards based on polygonal
interpolation. Maximum 20 data points (standards P1 up to P20)
can be used as given data points.

Measuring of standards:
Finish the procedure with [BREAK].
STORE ? Select [1] for storing all parameters of the method and finishing the procedure.
0/NO 1/YES Continue with 5.9 - Multi-standard tools.

Editing of standards:
General operation:
With [+] or [-] skip to the next data point.
With [Z] the selected data point is deleted. The values of A and C show: -00000
With [ENTER] the input of the current value is accepted.
With [BREAK] the procedure of input is finished.

A -00000 P 1 Enter the values of the first standard P1:

C -00000 EZB+- In the first line the absorbance A (e.g. 0.3) has to be entered. Confirm input with
[ENTER].
Select second line of display with [ENTER].
In the second line the concentration or activity C (e.g. 60) has to be entered.
Confirm input with [ENTER].

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Select next standard P2 with [+] or [-].

A -00000 P 2 Enter the values of the second standard P2 (see above).

C -00000 EZB+-

Select next standard P3 with [+] or [-].

A -00000 P 3 Enter the values of the third standard P3 (see above).

C -00000 EZB+-

Continue with inputs including the last standard. Maximum 20 standards can be
entered.
Finish the input with [BREAK].

STORE ? Select [0] to leave method editor without changing parameters.

0/NO 1/YES Select [1] to store all parameters of method and to finish the operation:
In case of storing the set of data points will be sorted by ascending absorbance.

A printing of data table and curve can be done (see chapter 5.9 - Multi-standard
tools: Graphic print-out of curve).

Example of a calibration curve:

The given data points (standards/calibrators P1 up to P20) have to be entered.
P1 is the standard with the lowest absorbance. P20 is the standard with the
highest absorbance.

Table showing an example of five given data points

Data point Absorbance A Concentration C
P1 0.302 60
P2 0.600 30
P3 0.920 15
P4 1.160 7.5
P5 1.501 3.25

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8 UTILITY PROGRAMS
8.1 SELECTION OF UTILITY PROGRAMS

METHOD NO. Method selection: change to utility program selection with [MODE].
INPUT 1-250
Confirm with [MODE].

INPUT 1-n/+ Utility program selection: direct selection of required utility program, i.e. input of
UTILITIES concerning identity no. (see table) and confirming with [ENTER], or by scrolling
through all utility programs with [+] ... [+].

Break of utility program selection and return to program selection with [BREAK].

A utility program can be broken off with [BREAK] at any time with return to the
selection of utility programs.

Automatic return to utility program selection at the end of a utility program.

Identity-No. Utility Program Description

in chapter

1 Dark level adjustment 8.2.1

2 User name 8.2.2
3 Error list 8.2.3
4 Deleting method 8.2.4
5 Language selection 8.2.5
6 Beep ON / OFF 8.2.6
7 Temperature control ON / OFF 8.2.7
8 Temperature calibration 8.2.8
9 AD-Transducer 8.2.9
10 ADC dark current values 8.2.10
11 Display test 8.2.11
12 Printing device configuration 8.2.12
13 Real time clock ON / OFF 8.2.13
14 Filter wheel test 8.2.14
15 Temperature correction 8.2.15
16 Printing user defined methods 8.2.16
17 Laboratory name 8.2.17

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8.2 DESCRIPTION OF UTILITY PROGRAMS

8.2.1 Dark level adjustment

The dark level adjustment should be performed only if the warm-up time of 15 minutes has passed, better after
one hour operation.
To avoid strange light the lid must absolutely be closed during the dark level adjustment.

Start menu by [MODE] [MODE] [1]:

DARK ADJ. U1 Confirm with [ENTER].

UTILITIES

DARK ADJ. U1 Automatically all eight sensitivity levels (V0...V7) are calibrated in succession at
V0 82 simultaneous display of the dark value.

8.2.2 User name

The names of maximum five users can be stored permanently.
In case of a selected user in the range from 1 to 5 an additional line within the header is sent to the printer or the
EDP. After invoking a method the course asks for the current user:

USER NO. +- 1 The last user is the default name (e.g. Tom).
Tom Select current user from 0 up to 5 by [+] or [-].
In case of selecting user 0 no additional line is in the print-out.
Confirm with [ENTER].

Installing of new user name

Start menu by [MODE] [MODE] [2]:

USER NAME U2 Confirm with [ENTER].

UTILITIES

USER NAME +- 1 The display shows the name of user number 1.

Select current user from 1 up to 5 by [+] or [-].

USER NAME +- 2 Edit the name of the user number e.g. 2 in the second line.
Maria For a description of input of names see chapter 7 – METHOD EDITOR.

Confirm with [ENTER].

8.2.3 Error list

With this utility the last 10 errors are shown or printed.

Start menu by [MODE] [MODE] [3]:

ERROR LIST U3 Confirm with [ENTER] for showing the errors subsequently
UTILITIES or confirm with [LF] for printing the errors.

In case of [ENTER]:
ERROR LIST U3
The error no. 10 is dated 07.23.01. The error code is 8.
10*07.23.01* 8 Continue with [ENTER] to show next error.
The error no. 1 is the error occurred at last.

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8.2.4 Deleting method

With this utility program one of the methods edited by the user can be deleted.

Start menu by [MODE] [MODE] [4]:

METHOD CLEAR U4 Confirm with [ENTER].

UTILITIES

Enter number of the method to be erased in the range of 20 up to 249.

METHOD NO. After confirmation with [ENTER] the method in question is immediately erased
INPUT 20-249 without further query.
query

8.2.5 Language selection

With this utility program the language of the display and print-out can be selected.

Start menu by [MODE] [MODE] [5]:

LANGUAGE U5 Confirm with [ENTER].

UTILITIES

Select English language with [1] or German language with [2].

1/GB 2/D The selected setting is active immediately.
UTILITIES

8.2.6 Beep ON / OFF

The beeper can be switched on or off. The deeper signal tone for error messages remains active in every case.

Start menu by [MODE] [MODE] [6]:

BEEP ON/OFF U6 Confirm with [ENTER].

UTILITIES

The current status is displayed in the upper line.

BEEP ON Switch off the signal tone with [0].
1/ON 0/OFF Switch on the signal tone with [1].

8.2.7 Temperature control ON / OFF

The electrical heating on 37 °C can manually be switched on or off.

Start menu by [MODE] [MODE] [7]:

TEMP. ON/OFF U7 Confirm with [ENTER].

UTILITIES

The actual status to the heating is displayed in the upper line.

HEATING OFF Switch heating ON with [1] and OFF with [0].
1/ON 0/OFF

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8.2.8 Temperature calibration

Attention: The temperature control was adjusted at the factory. In case of e.g. lost of data the heating
control can be calibrated if the heating is switched on (for at least 30 minutes):
Measure the current temperature with an independent measuring system (e.g. thermistor, REF 090-063) inside
the cuvette and enter this value. According to the difference to 37.0 °C the system corrects its internal setting.
The calibrating of the temperature is interrupted when the heating is off or the temperature unstable.

Start menu by [MODE] [MODE] [8]:

TEMP. CALIBR. U8 Confirm with [ENTER].

UTILITIES

Enter the password “4040” (as query). Confirm with [ENTER].

TEMP. CALIBR. U8
PASSWORD

Enter the temperature currently measured always four-digit in °C (e.g. 37.20).

INPUT DEGREE C
37C 37.20

8.2.9 AD-Transducer readings

Display of the current readings of the analogue-digital-transducer (proportional to the brightness on the photo
detector) in dependency on the selected sensitivity level.

Start menu by [MODE] [MODE] [9]:

ADC COUNTS U9 Confirm with [ENTER].

UTILITIES

Boost operation is deactivated. Activating with [1], deactivating again with [0].
BOOST OFF Accept the displayed status with [ENTER].
1/ON 0/OFF

ADC COUNTS U9 On the second line the current sensitivity level (e.g. 0) in the range 0…7 is shown
0 4711 F1 on the left and the current AD-transducer value (e.g. 4711) in the middle. On the
right the actual filter position (e.g. F1) is shown.

The current sensitivity level can be changed by numeric keys [0] up to [7].

The filter position can be changed by [MODE] and a numeric key [1] up to [9].
Filter positions 1 up to 9 are used for interference filters.
Filter position 0 is used for dark position of filter wheel.

8.2.10 ADC dark current values

Display of the offset values for the AD-transducer, determined and saved at the dark level adjustment, in
dependency on the selected sensitivity level.

Start menu by [MODE] [MODE] [10]:

ADC DARK U10 Confirm with [ENTER].

UTILITIES

On the second line the sensitivity level in the range 0…7 is shown on the left.
ADC DARK U10 The corresponding saved dark values are shown.
0 18300
Selection of another sensitivity level by pushing the corresponding number key.

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8.2.11 Display test

The efficiency of the display can be checked.

Start menu by [MODE] [MODE] [11]:

DISPLAY TEST U11 Confirm with [ENTER].

UTILITIES

A black rectangle appears at each digit of the display.

AY TEST U11 At the end of the operation each pixel is activated.
UTILITIES After working off the utility program confirm with [ENTER].

8.2.12 Printing device configuration

Print-out of the program version and the complete status of the stored variables.

Start menu by [MODE] [MODE] [12]:

PRINT DEF. U12 Confirm with [ENTER].

UTILITIES

******ACTUAL*SETS*******
DATE: 03.04.07
TIME: 16:25:27
PHOTOMETER 4040 # 1000
V2.3a 03.04.07 D /1.0
PCB LAYOUT a
ADC DARK
0: 17669 1: 17671
2: 17675 3: 17680
4: 17689 5: 17706
6: 17736 7: 17789
TEMPERATURE REQUIRED
37C 14000 3000
FILTER
0: DDD 1: 340
2: 405 3: 492
4: 546 5: 578
6: 623 7: 999
8: 999 9: 999
BATTERY: OK
ADC CORRECT. 75
BOOST NM 390
EDP ON STX-ETX-BCC
LANGUAGE GB
BEEP ON
PROGR. METHODS 0

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8.2.13 Real time clock ON / OFF

The real time clock can be switched OFF and ON. Date and time can be set.

Start menu by [MODE] [MODE] [13]:

CLOCK ON/OFF U13 Confirm with [ENTER].

UTILITIES

The real time clock is activated. Deactivating with [0], activating again with [1].
CLOCK ON Accept the displayed status with [ENTER].
1/ON 0/OFF

After deactivating and activating real time clock:

DATE mm.dd.yy Enter month, day, year, hour, minute and second. Confirm each entry with
TIME hh:mm:ss [ENTER].

Enter month, day, year, hour, minute and second. Confirm each entry with
DATE 07 25 01 [ENTER].
TIME 20 11 00
Confirm with [ENTER].

8.2.14 Filter wheel test

The filter wheel can be tested.

Start menu by [MODE] [MODE] [14]:

F-WHEEL TEST U14 Confirm with [ENTER].

UTILITIES

Select filter position in the range of 0 up to 9.

F-WHEEL TEST U14 Accept the displayed status with [ENTER].
EDIT 0-9

On the second line the current filter position (e.g. F2) and the stored wavelength
F-WHEEL TEST U14 (e.g. 405 nm) is shown.
F2 405NM 47MS Finish the test with [BREAK].

8.2.15 Temperature correction

Display of the current setting. The correction of the temperature regulation is possible by change of the
temperature setting. A change of about 25 counts means a temperature change of 0.1 °C.

Start menu by [MODE] [MODE] [15]:

TEMP. CORR. U15 Confirm with [ENTER].

UTILITIES

Enter new temperature value and confirm with [ENTER] or finish operation with
REQUIRED 37C
[BREAK]. The display shows for example the value 3000 (default) for the
3000
temperature level 37 °C.
Finish the test with [BREAK].

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8.2.16 Printing user defined methods

Print-out of all user defined methods. Each method is shown in two lines. The first line notes the method number
and the name of the method. The second line notes factor (F) or standard (S) and the value, the
wavelength (nm) and the dimension.

Start menu by [MODE] [MODE] [16]:

PRINT METH. U16 Confirm with [ENTER].

UTILITIES

METHOD 20: Test 1

F 1234 340nm mg/dl

METHOD 25: Test 2

S 5.555 578nm mmol/l

METHOD 30: Test 3

F 1050 405nm U/l

METHOD 250: C/F

F 1.000 0 nm

8.2.17 Laboratory name

The name of the laboratory can be stored permanently.
In case of a stored name an additional line within the header is sent to the printer or the EDP.

Start menu by [MODE] [MODE] [17]:

LAB. NAME U17 Confirm with [ENTER].

UTILITIES

LAB. NAME U17 Edit the name of the laboratory in the second line.
xxxxx For a description of input of names see chapter 7 – METHOD EDITOR.

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9 MAINTENANCE
This chapter informs the user about necessary maintenance.

If no error-free operation of the device can be achieved, the service should be informed. Repairs at the
device may be accomplished only by authorized technical personnel. In addition inappropriate repairs endanger
the operator and lead to expiring the warranty.

9.1 CLEANING INSTRUCTION

Liquid waste is potentially biologically hazardous. Always wear gloves if handling those materials. Do not
touch parts of the device other than those specified. Consult the laboratory protocol for handling biohazardous
materials.

Take care that no liquid runs into the device! There is no protection against penetrating liquids
(Code IP X0).

For device cleaning and surface decontamination purposes use commercial decontaminating solution which are
® ® ®
usually available in clinical chemistry laboratories like Mikrozid AF Liquid, Bacillol plus, 3 % Kohrsolin or
similar solutions. Switch off the device and disconnect it from the mains voltage. Then clean the device with soft
cloth and decontaminating solution.

9.2 CALIBRATING MEASURING SYSTEM

With doubtful results a dark level adjustment must be performed as described in chapter 8.2.1.
It is recommended to calibrate the device every four weeks to ensure an optimal operation.

9.3 CHANGING RIBBON

Open printer cover. Lift used ribbon by pressing “PUSH” on the left. Take ribbon cassette out of the printer.
Take new ribbon cassette out of the wrapping. Turn small wheel on the left in the direction marked by an arrow
till the inked ribbon is tightened.
Put in the ribbon cassette. Pay attention that the paper is to be taken through inked ribbon and cassette. Take
printer paper through the crack of the printer cover and close the printer cover.

9.4 CHANGING PAPER ROLL

See chapter 2.4 – LOADING PRINTER PAPER.

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9.5 REPLACEMENT OF LINE FUSES

The Photometer 4040 operates at any line voltage between 90 VAC and 264 VAC at 50/60 Hz without adjustment.
It has two line fuses in series with the power supply. These fuses are located on the rear panel. To replace
these fuses, unplug the mains cable and remove the fuse holder with the fuses as shown below. The instrument
is delivered with two spare fuses.

Neither use makeshift fuses nor short-circuit the fuse holder!

Specifications of mains fuse:

• dimensions [mm] : 5 * 20
• standard: IEC 60127-2/V
• time-relevant characteristic: time-lag (T)
• voltage value: 250 V
• current value: 1.6 A
• marking: T 1.6 A H

mains switch

fuse holder

mains cable connector

fuse holder with fuses

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10 ERROR MESSAGE / CORRECTION

10.1 GENERAL NOTE

Erroneous input caused by the user (e.g. wrong method number or wrong factor) can be corrected by filling the
input field with any signs beyond the last position. The erroneous input is deleted and the input field is ready for
the correct input.

Error messages by the device are carried out either exclusively via a signal tone (chapter 10.2 – ACOUSTIC
ERROR MESSAGES) or as combination of signal tone and display (see below).

1 0,005 R[ ] Errors are displayed as plaintext (chapter 10.3 – PLAINTEXT ERROR

ERROR MIN.UNITS MESSAGES)

METHOD NO. 300 … or coded with an error number (chapter 10.4 – CODED ERROR
ERROR 3 MESSAGES).

Each error message has always to be confirmed with [ENTER].

10.2 ACOUSTIC ERROR MESSAGES

Pressing a key not permitted or not expedient is marked by a deeper beep after the higher beep. (The higher
beep confirms the keystroke and can be switched off according to chapter 8.2.6 – Beep ON / OFF). A
corresponding error message is not displayed. The operation of the device can directly be continued by the
correct keyboard entry.

10.3 PLAINTEXT ERROR MESSAGES

ERROR MIN.UNITS The programmed low limit was under-run by the measurement.

ERROR MAX.UNITS The programmed upper limit was exceeded by the measurement.

ERROR R^2 At kinetic measuring the square of the correlation coefficient r lies below the
programmed low limit.

ERROR +/- At kinetic measuring the course of the kinetic is wrong (increasing / falling).

NO METHOD Selected method is not programmed. Select other method according to method
list.

HEATING OFF Heating is off during temperature calibration.

TEMP. UNSTABLE Temperature is unstable during temperature calibration.

10.4 CODED ERROR MESSAGES

No. (Possible) Causes Remedy

1 method is write protected, method can not be cleared by special software

2 check sum of a freely programmed method is wrong program new method
3 forbidden input, wrong number format repeat input in permitted area
4 method not available method editor: check method no.

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5 dark value is absolutely too high (> 16 bit) or higher as the repeat dark level adjustment;
measurement, check lamp / filter; check blank
ADC overflow
6 all multiplexer positions are too bright/too dark at setting to repeat dark level adjustment;
zero check filter / lamp; check blank
7 mathematical overflow at measurement calculation check filter; check standard;
check measuring solution
8 check sum error in the data record of the dark offset repeat dark level adjustment
9 check sum error in the data record of the device basic setting automatic error remedy
(status, ADC correction)
10 division by a too small value (< 0.001 A) check filter; check standard; check
measuring solution
11 invalid calibration curve Select valid number
12 setting to zero not possible (zero value is < 32768 cycle) check lamp; check filter;
check zero solution
13 setting to zero not possible (zero value is > 983039 cycle) check lamp; check filter;
check zero solution
14 invalid standard measure valid standard solution
15 no parameter memory vacant (too little memory for nonlinear delete a no longer actual nonlinear
methods) method
16 method no. is occupied select other method no.; delete a no
longer actual nonlinear method
17 check sum error in the parameter memory (nonlinear method) program method newly
18 at calculation overflows in nonlinear method check factor; check parameter
19 clock malfunction
20 overflow at Kinetic check measuring solution
21 overflow at Kinetic check measuring solution
22 overflow at Kinetic check measuring solution
23 overflow at Kinetic check measuring solution
24 overflow at Kinetic check measuring solution
25 overflow at Kinetic check measuring solution
26 overflow at Kinetic check measuring solution
27 overflow at Kinetic check measuring solution
28 overflow at Kinetic check measuring solution
29 wrong input of deltas or time per delta restart method
30 battery empty contact service partner
31 communication: wrong data format contact service partner
32 communication: sent data not plausible / not interpretable contact service partner
33 communication: mentioned module does not answer in a check interconnecting cable; check
certain time mentioned module
34 communication: overflow send/receive buffer reduce amount of data at the
communication partner
35 Remote control: wrong method number external software problem
36 Remote control: unknown command external software problem

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37 Remote control: wrong data format external software problem

38 timeout at reception from module 1 switch off/on device
39 check sum error at reception from module 1 switch off/on device
40 timeout at reception from module 2 switch off/on device
41 check sum error at reception from module 2 switch off/on device
42 NAK at reception from module 2 switch off/on device
43 NAK at reception from module 1 switch off/on device
46 filter position outside tolerance
47 lamp voltage outside tolerance contact service partner
52 timeout printer internal the internal printer is temporarily
disconnected
53 Set of data points is missing Check multi-standard functions
54 nonlinear measurement: value out of range check the absorbance of the sample
55 number of given data points < 2 add data points
56 checksum of operating system damaged contact service partner
57 memory of patients data management is completely filled delete patient data
(OM chapter 5.10.2)
58 checksum of patients data damaged delete patient data
(OM chapter 5.10.2)

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11 TECHNICAL DATA

11.1 ENVIRONMENTAL CONDITIONS

Climatic conditions for storage and transport of the packed device:
• Temperature: -25 °C to +70 °C
• Relative humidity: 20 % to 85 %

The Photometer 4040 must be used in an environment that meets the following conditions:
• Temperature: +15 °C to +35 °C
• Relative humidity: 20 % to 85 %
• Not exposed to direct sunlight or other source of direct light (e.g. a spot light)
• Well-ventilated area
• Free from excessive dust
• Free from combustible gases
• Free from vibrations
• Free from electromagnetic wave interference
• Well-distanced from a machine generating a high frequency voltage (e.g. a centrifuge)

11.2 MINIMAL OPERATION QUALITY

Signal processing in analogue amplifiers with high amplification factors cannot differentiate desired from
undesired signals. Amplifiers thus are apt to be overloaded or produce spurious signals. The equipment will
operate correctly when the undesired signals are removed. Short- term changes of the operational behavior do
not influence the overall function of the device.

11.3 TYPE PLATE

For installation pay attention to the specifications on the type plate.

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11.4 SHORT SPECIFICATION

Type Semi-automatic, single-beam filter photometer
Light Source Halogen lamp – 12 V, 20 W
Wavelength 340 nm – 800 nm
Wavelength Selection Automatic via 9-position filter wheel :
6 standard interference filters: 340 nm, 405 nm, 492 nm, 546 nm, 578 nm
and 623 nm; 3 positions for optional filters of choice
Photometric Range 0 – 2.5 A
Cuvette System Standard cuvettes (macro or semi-micro, disposable or special optical glass)
Temperature Controlled Measuring cell as well as incubation block with 2 positions temperature
Cuvette Compartment controlled by electrical thermostat at 37 °C
Operator Interface Membrane keyboard with 20 keys for direct functions and numeric entries
Data Presentation LCD display: lighted, 2 lines of 16 characters each, easy to read and offering
simple user guidance
Integrated Printer Dot-matrix printer, 24 characters per line
Memory General operating software contained in FLASH MEMORY can be updated
by PC (data file downloaded from Internet, floppy disk or CD ROM)
Languages: English and German (other languages on request)
Capacity for 250 pre-programmed test variants
Up to 230 tests can be stored. Import of data by keyboard or PC
Up to 50 nonlinear calibration curves with max 20 sets of points can be stored
Signal Port RS 232 serial port for connection to an external printer and/or computer
(bidirectional interface)
Measurement ƒ Absorbance
Procedures ƒ Endpoints with standard, factor or multiple standards, with or without
reagent blank
and/or sample blank
ƒ Kinetics with standard, factor or multiple standards, with or without blank
ƒ Fixed time with standard, factor or multiple standards, with or without
reagent blank
ƒ Curve fitting for nonlinear standard curves
Measuring Time ƒ Kinetic: variable from 3 – 28 deltas,
time per delta 4 – 255 sec in 1 sec steps
ƒ Fixed time: variable from 0 – 65535 sec in 1 sec steps
Delay Time Programmable from 0 – 65535 sec in 1 sec steps
Dimensions Length 33 cm x width 34 cm x height 18 cm
Weight 5.6 kg

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11.5 CERTIFICATES

EC Declaration of Conformity

Manufacturer: ROBERT RIELE GmbH & Co KG

Address: ROBERT RIELE GmbH & Co KG

Kurfuerstenstrasse 75-79
D-13467 Berlin
Germany

ROBERT RIELE GmbH & Co KG declares under sole responsibility that the product:

Product name: Photometer 4040

to which this declaration relates is in conformity with the following standards or other normative documents:

EN 61326-1

EN 61010-1

EN 61010-2-101

following the provisions of Directive 89 / 336 / EWG (EMC) and 73 / 23 / EWG (LVD) as well as
98 /79 / EG (IVD).

This declaration describes an absorption photometer for laboratory use.

Berlin, November 2004

ROBERT RIELE GmbH & Co KG

W. Riele

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Technical Specifications
Description according to DIN 58960 part 4

A Identification

A.1 Type of photometer: Photometer 4040

A.2 Model: 4040

A.3 Instruction for use: Photometer 4040, user manual

A.4 Manufacturer ROBERT RIELE GmbH & Co KG

Kurfuerstenstrasse 75-79
D-13467 Berlin
Germany

DECLARATION OF CONFORMITY:
The above mentioned absorption photometer is in conformity with the following metrological description.

Berlin, November 2004

ROBERT RIELE GmbH & Co KG

W. Riele

B Metrological description

B.1 Measuring System

B.1.1 Optical configuration: cf. Figure
B.1.2 Source[s] of radiation: halogen vapor lamp
B.1.3 Spectroscopic apparatus: filter wheel
B.1.4 Radiation detector[s]: photodiode
B.1.5 Cuvette[s]/cell[s]: 10 mm glass or plastic cuvette (square
shaped)
B.1.6 Temperature regulation of the cuvette: 37 °C
B.1.7 Displayed units: absorbance, mass concentration,
enzyme activity
B.1.8 Display device: digital display,
absorbance: 0.000 to 2.500
mass concentration: 0.000 to 9999
enzyme activity: 0.000 to 9999

B.2 Method of measurement

B.2.1 Generation of spectral absorbance A(λ) monochromatic measurement
B.2.2 Zero compensation of spectral absorbance manual
B.2.3 Control of the measured spectral absorbance: with an absorption reference
solution (see manual)
B.2.4 Determination[s] of concentration: Lambert-Beer-Equation

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B.3 Specified measuring range

Outside the specified measuring range and under rated operating conditions other than those
stated in section B.4, the maximum permissible errors given in section B.5 can be exceeded.

B.3.1 Spectral absorbance A(λ): 0 A to 2.5 A

B.3.2 Wavelength λ useable for measurements: 340 nm to 800 nm

B.4 Specified Operation conditions

B.4.1 Spectral transmittance of the cuvette: > 75 %
B.4.2 Warm-up time: 15 min
B.4.3 Operating voltage [mains]: between 90 VAC and 264 VAC at 50/60 Hz
B.4.4 Ambient temperature: 15 °C to 35 °C
B.4.5 Sound pressure level SPL < 50 dB

B.5 Maximum permissible errors and other limiting values

B.5.1 Photometric uncertainty of the spectral absorbance: max ± 3 %
B.5.2 Photometric short-time variation coefficient: ≤1%
B.5.3 Uncertainty of wavelengths: max ± 2 nm
B.5.4 Spectral half-width of spectral radiation flux at
detector: ≤ 10 nm
B.5.5 Percentage of wavelength integrated false
radiation ( measured at 340 nm as transmittance
of a cut-off filter NaNO3): ≤ 0.1 %
B.5.6 Uncertainty of temperature regulation: ≤ 0.2 °C
B.5.7 Specimen related carry-over ratio:
measure according to Broughton with
p-Nitro phenol ≤3%

OPTIC CONSTRUCTION
The path of rays is directed from right to left. Put in single cuvette accordingly.

Diaphragm 1 Diaphragm 2 Diaphragm 3 Diaphragm 4

Lens 1 Lens 2 Lens 3 Lens 4

Halogen lamp

Photodiode Cuvette Filter Glass for heat absorption

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12 ACCESSORIES AND SPARE PARTS

Kindly contact directly the responsible dealer.

REF Description
1706853001 Axis printer paper
0573655001 Cuvettes of optical glass, 4 pcs
501-002 Data cable serial interface
805-410 Disposable cuvettes, 1000 pcs
1704818001 Dust cover
5010-018 Fuses for line power, 10 pcs
0554871001 Halogen lamp 12V/20W
500-002 Incubator T12
0369624001 Interference filter 436nm
5010-670 Interference filter 670nm
(other wavelengths on request)
0552402001 Mains cable
4040-005 Operator’s manual
1268457001 Printer paper, 5 rolls
1010735001 Printer ribbon, 1 pc
0745235001 Printer ribbon, 5 pcs
090-064 Secondary test set, four-part, certified
090-063 Thermistor
1707574001 Top cover small for printer

Incubator T12

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13 METHOD LIST

1 - 15..........15 basic methods (chapter 13.1 - BASIC METHODS)

16 - 19........free (reserved for further 4 automatic calculation methods)
20 - 249......up to 230 user specific methods (chapter 13.2 - LIST OF USER SPECIFIC METHODS as copy
master / to be filled out by the user)
250 .............method Absorbance with open parameters

13.1 BASIC METHODS

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13.2 LIST OF USER SPECIFIC METHODS

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