AMSCO® 110LS AND AMSCO® 250LS

SMALL STERILIZERS – LIFE SCIENCES

APPLICATION
AMSCO 110LS and AMSCO 250LS Small Sterilizers are
designed for use in laboratory and industrial applications.
Each is configured with pre-vacuum, liquid, and gravity
cycles. A full list of standard and optional cycles begins on
page 2.

DESCRIPTION
AMSCO 110LS and AMSCO 250LS Small Sterilizers for life
science applications are the next advancement in the STERIS
line of steam-jacketed sterilizers. Both sterilizers are
equipped with the latest features in state of the art technology
and ease of use.
Interior Chamber Dimensions
• AMSCO 110LS – 16 x 16 x 26" (406 x 406 x 660 mm),
110L capacity
• AMSCO 250LS – 20 x 20 x 38" (508 x 508 x 965 mm),
250L capacity

Allen-Bradley MicroLogix™ Control System with

enhanced functionality and user-friendly Allen-Bradley
PanelView Plus™ 7 700 interface screen.*
• Touch-sensitive screen with 18-bit color graphic display
(Typical - details may vary.)
• Display features 640 x 480 resolution color-active matrix

Selections Checked Below Apply To This Equipment

MODEL/CHAMBER SIZE (W x H x L) ❑ Effluent Decontamination Cycle ACCESSORIES (for AMSCO 250LS)
❑ Pure Steam Piping to Chamber ❑ One Intermediate Shelf (20 x 20 x 38")
❑ AMSCO 110LS
❑ Air Detector System ❑ Seismic Tie-Down Kit7,9
16 x 16 x 26" (406 x 406 x 660 mm)
❑ Reference Recorder ❑ Air Compressor, Portable, 115 Vac
❑ Prevacuum (LV-110) ❑ Isothermal (LI-110)
❑ Printer on Both Ends8 ❑ Loading Rack and Two Shelves (20 x 20 x 38")
❑ Bioseal 3 ❑ Single Door ❑ Double Door
❑ AMSCO 250LS ❑ Loading Car
❑ RTD Load Probe(s) and F0 Sterilization
20 x 20 x 38" (508 x 508 x 965 mm) ❑ Transfer Carriage
❑ Prevacuum (LV-250) ❑ Isothermal (LI-250) ❑ One Probe ❑ Two Probes (max. 2)
❑ Drain Line Reference Probe ❑ Chamber Track Assembly
❑ Air-Differential Seal (NOE) for Double Door Units ❑ Single Door ❑ Double Door
STEAM SOURCE ❑ Back Panel for Single Door Cabinet Enclosed Unit ❑ Loading Car, Transfer Carriage & Chamber Track
❑ Building Steam ❑ 1" Chamber Penetration, Qty. 1 Assembly
❑ Stand Alone Electric Steam Generator (AMSCO 110LS only)4 ❑ Single Door ❑ Double Door
❑ STERIS Provided ❑ Customer Provided ❑ One Additional Chamber Penetration
❑ Integral Electric Steam Generator1 (AMSCO 250LS)4 Notes:
❑ Carbon Steel ❑ Stainless Steel ❑ Vacuum Pump5 1. AMSCO 110LS double door sterilizers are not
Voltage Options ❑ 120 V, 1-Ph / 208 / 240 Vac, 3-Ph available with integral electric steam generator.
❑ 208 Volt, 60 Hz ❑ 120 V, 1 Ph / 240 Vac 3-Ph 2. Available for AMSCO 250LS double door
❑ 240 Volt, 60 Hz ❑ 120 V, 1-Ph / 480 Vac, 3-Ph sterilizers only. Contact engineering if mounting
❑ 400 Volt, 50 Hz through two walls is required for AMSCO 110LS.
❑ 120 V, 1-Ph / 600 Vac, 3-Ph
❑ 480 Volt, 60 Hz 3. Available on AMSCO 250LS double manual door
❑ 230 V, 1 Ph / 400 Vac, 3-Ph (International)
❑ 600 Volt, 60 Hz sterilizers only.
❑ Other - Specify ___________________
4. One chamber penetration is standard on
❑ Integral Indirect Stainless-Steel Clean Steam ❑ Dry Contacts AMSCO 250LS.
Generator (SD589)10 ❑ Backflow Preventer 5. 1-Ph is for control, 3-Ph is for pump motor
❑ Auto Flush for Steam Generator 6. Loading rack & 2 shelves standard on
❑ SINGLE DOOR ❑ 0.2 Micron Bacterial Retentive Filter AMSCO 110LS.
❑ Cabinet Enclosed/Freestanding ❑ Green Gravity Water Saver System 7. Based on CA requirements.
❑ Recessed ❑ STERI-GREEN® Water Conservation System 8. Printer at operating end is standard. Option adds
❑ STERI-GREEN PLUS® Water Conservation System additional printer to non-operating end.
❑ DOUBLE DOOR
(Chilled water required) 9. Units with Bioseal require special seismic kit.
❑ Recessed through One Wall
10. Integral indirect clean steam generator requires
❑ Sterile side ❑ Non-sterile side
field certification for UL/CSA.
❑ Recessed through Two Walls2 ACCESSORIES (for AMSCO 110LS)6
OPTIONS ❑ One Intermediate Shelf (16 x 16 x 26")
❑ Power Door(s) ❑ Air Compressor, Portable, 115 Vac Item ________________________
❑ Liquid Air Cool (w/vacuum) Location(s)___________________
____________________________
* Allen-Bradley MicroLogixTM and Allen-Bradley PanelView PlusTM are
registered trademarks of Rockwell Automation, Inc. SD1026 Rev B
• Display is designed with emphasis on human factors and Liquid Cycle (standard) provided on prevacuum, and
user recognizable symbols. isothermal sterilizers, for sterilization of liquids and media in
• Noise level for units with water ejector 68Dba, units with vented borosilicate glass or metal containers at 100°C to 141°C
vacuum pump 74Dba. (212°F to 285°F). Liquid cycle uses optimal solution cooling
feature, during exhaust (cooling) phase, to control exhaust rate.
Cycle Programming and Flash Memory
Prevacuum Cycle (standard) provided only on prevacuum
• 20 cycles may be individually selected and programmed sterilizer, for efficient, high-volume sterilization of porous, heat-
• Help screens for control operation and moisture-stable materials at 100°C to 141°C (212°F to
285°F). Prevacuum cycle utilizes a mechanical air-evacuation
• Program permanently stored in flash memory
system.
• Variables permanently stored in flash memory
USP 660 Cycle (standard) developed to assist meeting the
Vertical Sliding Door(s) requirements of USP 660 sterilization test cycle for glassware.
Load probe option is recommended when using this USP cycle.
AMSCO LS configurations include choice of single or double
door sterilizers and open or recessed mounting. The doors are Continuous Cycle (standard) This cycle allows for up to 9,999
manually operated. Door seals (1 per door) are non-lubricated, cycles to be run consecutively without the need of an operator.
steam activated. Cycle parameters can be set, along with the amount of time to
NOTE: Recess two wall mounting is not available for lapse in between cycles. For this cycle to function, a power
AMSCO 110LS double door sterilizers. door is required to facilitate automatic opening and closing.
Modularized Vessel And Piping Waste Bag Cycle (standard) This cycle has been
preconfigured with cycle parameters shown to be effective
Vessel and piping are designed for increased dependability
when processing lab waste in autoclavable bags. Through air
and reduced service time.
removal assistance and a controlled exhaust, the internal
• Reduced piping components increase reliability
temperature is increased more effectively and faster, while
• Vessel design allows higher operating temperature of 141°C minimizing boil over of any liquids present. Parameters may
(285°F)
need to be adjusted based on specific loads. Bags should not
• Non-clogging chamber drain line prevents media from be completely sealed.
plugging drain line
Isothermal Cycle (optional) provided only on isothermal
• Emergency manual exhaust valve sterilizer, for processing of heat-sensitive and heat-coagulable
solutions in vented borosilicate glass or metal containers at
STANDARDS 78°C to 110°C (170°F to 230°F). Isothermal cycle utilizes steam
to enhance temperature control and prevent layering of steam
Each sterilizer meets applicable requirements of the following and air within the chamber. Process maintains positive
listings and standards, and carries the appropriate symbols. pressure in chamber to inhibit media boiling.
• Governing Directives for Affixing the CE Mark: NOTE: Temperature control spread is greater on Isothermal
» Low-Voltage Directive (2014/35/EU) units ±6°C.
» EMC Directive (2014/30/EU) Liquid Air Cool (Optional) provides water to jacket, and air
» Machinery Directive (2006/42/EEC) pressure to chamber to improve exhaust time for liquid loads,
and to reduce boil-over.
• Pressure Equipment Directive (PED): 97/23/EC.
ATF 1 and ATF 2 Cycles (Alternating Tangential Flow) are
• UL/EN/CSA 61010-1: Safety Requirements for Electrical designed to sterilize the XCell™ ATF system. These can be
Equipment for Measurement, Control, and Laboratory Use used for other purposes that require rates and hold times in
Part 1: General Requirements the pre-conditioning pulses and a cool down in exhaust by
• UL/EN/CSA 61010-2-040: Safety Requirements For Electrical temperature.
Equipment For Measurement, Control, And Laboratory Use, Effluent Decontamination Cycle (Optional) is used for
Part 2-040: Particular Requirements For Sterilizers And Washer- processing contaminated laboratory waste (BL-3 and
Disinfectors Used To Treat Medical Materials BL-4). Condensate produced during processing cycle is
• ASME Code, Section VIII, Division 1 for unfired pressure decontaminated before discharge to floor drain. Steam is
vessels. The pressure vessel is so stamped; ASME Form U-1 admitted through bottom of sterilizer chamber, and chamber
is furnished. The shell and door are constructed to withstand is exhausted out top side of vessel. During purge and vacuum
a working pressure of 45 psig (3.1 bar). pulses, all purge and exhaust gases are vented through a
0.2 micron bacterial retentive filter. Filter housing is steam
jacketed to prevent wetting of filter membrane. Available with
CYCLE DESCRIPTIONS
fast exhaust or optimal solution cooling (slow exhaust) exhaust
types. User is responsible for development of process
Standard and optional cycles are as follows:
parameters.
Gravity Cycle (standard) provided on prevacuum, and
Leak Test Cycle, for verification of door seal and piping system
isothermal sterilizers, for sterilization of heat- and moisture-
integrity. Cycle parameters are pre-programmed and fixed.
stable goods at 100°C to 141°C (212°F to 285°F), and
Acceptable maximum leak rate is 1.0 mm Hg/minute over a 10-
decontamination of bagged non bio-hazardous laboratory
minute period following a fixed stabilization time.
wastes. Gravity cycle utilizes gravity air-displacement
principle. However, the gravity cycle has a standard drying
phase which uses a vacuum. The drying phase can be
disabled.

2
Daily Air Removal Test (Dart) Cycle, provided only on Resistance Temperature Detectors (RTDs) are installed for
prevacuum sterilizer, for verification of effective removal of sterilizer temperature control. The chamber drain line RTD
residual air in chamber and load during testing. Test cycle senses and controls temperature variations within the sterilizer
determines if even and rapid steam penetration into test load chamber. A jacket RTD provides temperature control within the
occurred. Cycle parameters are preprogrammed and fixed. jacket space. These RTD signals, converted into electrical
Bowie-Dick Test is available for 121°C (250°F) and 132°C impulses, provide accurate control inputs and readouts
(270°F) prevacuum cycles. throughout entire cycle.
Healthcare Cycles - The LS Series sterilizers can be Software calibration is provided for all temperature and
programmed to reproduce cycles that are used for healthcare pressure inputs. Calibration is performed in the service mode,
applications, however, these sterilizers do not have FDA accessible through the touch screen displays, and
clearance and cannot be used for patient treatment accomplished using external or internal temperature and
applications pressure sources. Control system provides a printed record of
all calibration data for verification to current readings.
ENVIRONMENTAL SUSTAINABILITY Cycle Data Records, recorded on the printer tape. They can
also be retrieved for on-screen review, USB, or sent via e-mail
if the system is enabled. The e-mail feature requires a network
Automatic Utilities Startup/Shutdown
connection.
This feature permits slow cooling of the entire vessel and load. Optimal solution cooling is designed to safely cool various
Shutdown may be programmed to activate at the end of any liquids in vented, borosilicate glass containers with minimum
designated cycle or time of day. When activated, control liquid loss due to boil-over, and to keep normal evaporation
system automatically shuts off all utility valves, conserving loss below 5%. Optimal solution cooling is an integral part of
steam and water usage. Sterilizer utilities can be restarted the factory-programmed liquid cycle. During the exhaust
either by programmed time or manual operation. A different (cooling) phase, the control utilizes this feature to optimize the
shutdown and restart time can be programmed for each day. exhaust rate regardless of load size or container fill volume, up
Green Mode to one liter. During cooling, the initial rate, initial rate transition
point, as well as the second rate and final vacuum level are
The Green Mode is a standard feature on the control that will adjustable.
shut off the steam to the jacket after the unit has sat idle for a
Steam purge feature is provided to assist in air removal and
specified period of time. The specific time frame is determined
preheat the load.
by the user and entered into the control during set up; it can
be changed at any time. Automatic steam shutoff to jacket is provided for isothermal
and liquid cycles. When activated for isothermal cycles, the
Green Gravity Water Saver System jacket control conducts a timed jacket drain, automatically
The Green Gravity Water Saver System provides additional allowing for the operation of cycles at lower temperatures.
water savings by collecting steam effluent and holding it in a When activated for liquid cycles, steam supply to the jacket is
cooling tank, reducing the amount of water required to cool the turned off during exhaust phase, allowing load to cool more
effluent. efficiently.
STERI-GREEN® Water Conservation System Insulation, one-inch thick, asbestos-free spin-glass (rated at
1000 °F [538 °C] continuous) encompasses the exterior of the
This system significantly reduces the consumption of potable sterilizer vessel and is sealed in an oil and water resistant outer
water. The STERI-GREEN system utilizes a mixing tank and an jacket.
air-cooled heat exchanger to cool and recycle vacuum pump Lighted DIN connectors are installed on all steam, water, and
water and steam effluent. Water temperature is constantly exhaust valves for reliability and ease of maintenance.
monitored to minimize the need to add fresh cool water to the
mixing tank. The system can provide up to 79% water savings Air Backup to seals is provided on all double door sterilizers,
over the vacuum pump alone, or up to 87% over a water ejector. with either bioseal or air differential seals.
When ordered, system includes a vacuum pump (3-phase Visible Gauges are standard. Chamber and jacket pressure
power required). gauges are standard on the operating end and non-operating
end.
STERI-GREEN PLUS® Water Conservation System
The STERI-GREEN PLUS system utilizes facility chilled water CONTROL SYSTEM
supply. The system utilizes a mixing tank and a series of heat
exchangers, integrated with the a chilled water loop, to cool Design Features
and recycle vacuum pump water and effluent. Water
temperature is constantly monitored to minimize the need to Together, the Allen-Bradley MicroLogix™ PLC control and
add fresh cool water to the mixing tank. This system provides PanelView Plus™ 7 700 display, monitor and control all sterilizer
up to 99% water savings over water ejector or vacuum pump operations and functions. Control system is factory-
upgrade alone. When ordered, system includes a vacuum programmed with standard sterilizing cycles, each adjustable
pump (3-phase power required). to meet specific processing requirements. All control
configuring is performed through touch screen displays.
STANDARD FEATURES Cycle values and operating features may be adjusted and
verified prior to cycle operation. Once cycle is started, cycles
Hinged front cabinet panel opens for convenient access to
and cycle values cannot be changed until cycle is complete.
sterilizer piping and control system.
On completion of cycle, timers reset to the previously selected
values, eliminating the need to reset values between repeated

3
cycles. If chamber temperature drops below the under display. In addition to adjustment of cycle values, the
temperature setting during the exposure phase, timer can be following operating parameters can also be changed
set to stop and automatically reset or resume once normal through the change values menu:
operating temperature is reached. » Time Display and Printout Units in standard AM/PM or
Critical control system components are housed within a sealed 24-hour military (MIL) time.
compartment to protect components from moisture and heat » Selectable Cycle Name permits user to name each
generated during sterilization process. cycle with any combination of letters, numbers, blank
The control system is provided with features such as audit trail, spaces, and underscores, up to eight characters long.
password management and electronic signatures, which can » Print Interval permits adjustment of the time period
between cycle-status printouts generated during the
sterilize phase.
» Security access code is required to enter the operating
mode (running cycles), supervisor mode (changing val-
ues), and service mode. Operating the sterilizer or
accessing change values menu causes display to
request the entry of an access code. If access code is
not properly entered, display returns to the standby
screen, denying user access to the sterilizer or program-
ming. Access to the sterilizer can be limited to 12
operators, each with a different access code.
» Alarm tones – The end of cycle and alarm tones have
adjustable volumes ranging from off, low, medium and
high. The alarm tone does not have the selection for off.
Touching the display buttons will create audible beep.
Typical Cycle Menu Display
» Temperature Display and Printout Units in Celsius
facilitate compliance to 21 CFR Part 11 (Code of Federal (°C) or Fahrenheit (°F). Temperature is set, displayed,
Regulations Title 21) controlled, and printed to the nearest 0.1°. Recalibration
is not required when changing temperature units from
Operator interface control panel, consisting of touch screen
°C to °F and vice versa.
and thermal printer, is located on operating (load or nonsterile)
end of sterilizer. If the sterilizer is equipped with double doors, » Pressure/Vacuum Display and Printout Units in
an additional touch screen is provided on the non-operating psi/In/Hg, Bar (Gauge and Absolute). Recalibration is
(unload or sterile) end. not required when changing pressure units.
• Touch-Sensitive Screen features a color active matrix
18-bit graphics display. All sterilizer functions, including Mobile and PC Messaging (Standard)
cycle initiation and cycle configuration, are operated by
pressing the touch-sensitive areas on the display, referred This is a standard feature on the control that allows the user to
to as buttons. Display indicates appropriate control buttons, receive text messages or emails alerts regarding the status of
operator prompts and status messages necessary to assist the autoclave. The operator can choose from a list of # possible
in sterilizer operation. All displayed messages are complete alerts from which to be notified.
phrases with no codes to be cross-referenced. Display also SCADA Ready Control Interface (Standard)
indicates any abnormal conditions that may exist either in
or out of a cycle. If requested, STERIS can provide the user the information to
allow communication between the autoclave and the Building
• Thermal printer is a high resolution (8 dots per mm) printer. Management System. An ethernet connection is required.
It is fast and quiet, printing at 25 lines per minute on industrial
grade thermal paper. The device provides an easy-to-read Printer Data Storage (Standard)
printed record of all pertinent cycle data on 2.25" (57 mm) All printer data is saved internally and can be retrieved and
wide paper. Data is automatically printed at the beginning viewed on the display screen, exported to a USB drive or
and end of each cycle and at transition points during the emailed to the User.
cycle.Three paper tape rolls are furnished with each unit.
Printouts have a guaranteed lifetime of 25 years and are Cycle usage (Standard)
resistant from exposure to steam, alcohol, UV and visible Utilization of the sterilizer can be monitored for each User, data
light, oil, heat and water. can be exported via an Ethernet or USB port.
• Non-operating end (NOE) control panel (equipped on
double-door sterilizers only) includes a touch-sensitive
screen similar to the operating end screen. Preprogrammed SAFETY FEATURES
cycles can be started from the NOE control panel. Display
concurrently shows the same information as the operating Control lockout switch (equipped on chamber door) senses
end screen display. when door seal is energized and tight against the door. Control
prevents cycle from starting until the limit switch signal is
• Cycle configuration is performed by accessing the change received. If control loses appropriate signal during cycle, alarm
values menu on either end of a double door sterilizer. Utility activates, cycle aborts, and chamber safely vents with a
shutdown parameters can only be changed on the OE controlled exhaust.
4
Chamber float switch activates alarm, aborts cycle, and safely into the water-supply system and sterilizer. An optional
vents chamber with a controlled exhaust if excessive backflow preventer is available. Water supply shutoff valve is
condensate is detected in the vessel chamber. located behind the front cabinet service panel under the
Door interlocks (double door units only) allow only one door chamber.
to be opened at a time and, during processing, prevent the Automatic Drain Effluent Cooling
non-operating end (NOE) door from being opened until a
satisfactory cycle is complete. If a cycle is aborted, the NOE The piping system to the drain provides automatic condensing
door cannot be opened. The use of this feature may affect the of chamber steam and disposal of condensate to waste.
door gaskets life expectancy unless an air differential or bio Cooling water is added to ensure discharge temperature is
seal is provided. discharged at or below 60°C (140°F). A separate resistance
temperature detector (RTD) is included to limit the volume of
Pressure relief valve limits the amount of pressure buildup so
water to only the amount required to achieve target
that the rated pressure in the vessel is not exceeded.
temperature, thus conserving water.
Emergency stop button (located on front of the sterilizer) is
Vacuum System
included on all sterilizers. A key is required to release the
emergency stop (once pushed) before the unit can return to Chamber pressure is reduced during the conditioning phase
normal operation. and drying phase through the means of either a standard water
ejector or an optional vacuum pump upgrade. Subsequent to
CONSTRUCTION the drying phase, the chamber is returned to atmospheric
pressure by admitting air through a bacteria-retentive filter.
Shell Assembly
Two fabricated Type 316L stainless-steel shells, welded one Steam Source
within the other, form the sterilizer vessel. Type 316L stainless- Sterilizers are piped valved, and trapped to receive building-
steel end frame(s) is welded to door end. On single door units, supplied steam delivered at 50 to 80 psig (344.7 to 551.6 kPa)
back of chamber is fitted with welded, 316L stainless-steel dynamic. If building steam source is not available, an electric
formed head. carbon-steel steam generator or electric stainless-steel steam
Sterilizer vessel is ASME rated at 50 psig (3.2 Bar) and generator may be provided to supply steam to the sterilizer.
insulated. The Vessel (for AMSCO 250LS only) includes one Steam piping is constructed of brass and includes a shutoff
1.0" (25 mm) NPT welded chamber bushing for Customer use. valve, steam strainer and a brass pressure regulator.
Steam-supply opening inside the chamber is shielded by a Optional stainless-steel indirect-type steam generator can be
Type 316L stainless-steel baffle. installed as a pure steam source. Pure steam reduces the
Chamber Door(s) probability of contamination which could adversely affect
research, such as tissue culture and trace metals studies.
Door is constructed of a single formed piece of Type 316L Steam feeds from the jacket to the chamber. A check valve is
stainless steel. Door is insulated to reduce the surface added between the jacket and chamber on sterilizers with
temperature of the stainless-steel door cover. decontamination cycle option.
During cycle operation, door is sealed by a steam-activated
door seal. Door seal is constructed of a special long-life rubber Piping
compound. When sterilizer cycle is complete, the seal retracts
under vacuum into a machined groove in sterilizer end frame. All piping connections terminate within the confines of the
Door seal can be manually retracted to open the door and sterilizer and are accessible from the front and side of sterilizer.
remove critical load in an emergency situation (if loss of vacuum • Solenoid Valves in manifold with DIN connectors simplify
or loss of power occurs). sterilizer piping and can be serviced individually.
A handle is used to manually open (by lowering) and manually • Manual Shutoff Valves are pressure rated at 125 psig
close (by raising) the door which is suspended by cables (862 kPa) for saturated steam. Valve handles are low-heat
attached to a counterweight. conducting.
A long-life proximity switch is used, by the control, to determine
if the door is closed. An additional seal pressure switch MOUNTING ARRANGEMENT
prevents inadvertent cycle initiation if door is not sealed.
The door assembly is equipped with a mechanical locking Sterilizers are arranged for either freestanding or recessed
mechanism that ensures the door cannot be opened, as long installation, as specified. Each sterilizer is equipped with a
as the seal is intact and energized, and more than 2.0 psi height-adjustable, steel floor stand. Sterilizer subframe is
pressure is in the chamber. Door interlocks on double door equipped with a synthetic rubber gasket to ensure tight fit
sterilizers can be programmed to prevent inadvertent opening between the cabinet panels on freestanding units or between
of door(s). Access code is required to override door interlocks. the front cabinet panel and wall partition on recessed units.
On freestanding units, stainless-steel side panels enclose the
NOTE: Bio-Seal option is available for AMSCO 250LS, double sterilizer body and piping.
manual door sterilizers, as discussed on page 5. Each AMSCO 110LS and AMSCO 250LS sterilizer has a
lockable front service panel.
Chamber Drain System
Drain system is designed to prevent pollutants from entering

5
OPTIONS Power door provides automatic opening and closing by
depressing a foot pedal. (Not available for sterilizers with
Stainless-steel piping to chamber delivers steam generated Bio-Seal option).
from Customer purified water source to the chamber and its Vacuum Pump Upgrade: Water ejector is replaced with a
contents. All steam-to-chamber piping components are water ring vacuum pump.
constructed of 300 series stainless steel. Option is provided
Drain line reference probe automatically senses the drain line
with Pressure Reducing Valve. Compressed air required for
temperature during cycle operation.During the Sterilize phase,
pneumatic valves.
the chamber and reference probes are compared and if the
Integral indirect stainless-steel clean steam generator difference is outside the allowable range, an alarm will occur.
automatically produces clean steam using Customer-supplied This option replaces the standard drain RTD probe with a dual
steam and purified water. Generator is integrally connected to element RTD probe, in the same sheathing.
the clean steam-to-chamber piping system.
0.2 micron bacterial retentive filter provides sterile air during
30 kW carbon-steel electric steam generator typically fed by airbreak at end of cycle.
a potable water source with hardness not to exceed 171 mg/L.
Reference recorder: An optional independent recorder is
The generator is available for both single and double door
provided to record chamber drainline temperature and
sterilizers. The generator is mounted underneath both single
chamber pressure. The recorder is remote mounted.
and double door units. The generator option is not available
for AMSCO 110LS double door units. Additional chamber penetration: One 1" NPT capped
chamber penetration port is located at the side of the vessel
30 kW electric stainless-steel steam generator is electrically
so as not interfere with other piping. The port provides for up
powered, automatically filled with water having 1 M·cm
to twelve (12) owner provided thermocouple probes or other
resistivity, and operates whenever the sterilizer power is on.
test instrumentation.
Generator is integrally connected to the clean steam-to-
chamber piping system. The generator option is not available One port is provided as standard on AMSCO 250LS.
for AMSCO 110LS double door units. Printer on both ends. An additional printer is provided on the
Auto Flush for Steam Generator provides automatic flush of non-operating end of the sterilizer.
steam generator upon startup of sterilizer. Not required for SS Dry Contacts provide four (4) relays to communicate the
generators. following equipment status: door open, door closed, alarm, and
RTD load probes and F0 sterilization (maximum of 2) unit on.
automatically sense the load temperature during cycle
operation. A single thermal load probe is sealed through the ACCESSORIES
sterilizer vessel and manually placed in the product container
within the chamber prior to cycle operation.
In conjunction with the load probe option, individual cycles can Air Compressor, Portable, 115 Vac. This accessory is
be set to start exposure phase according to chamber drain intended for pneumatic valves on sterilizers when an air utility
temperature or according to load temperature. Also, F0 set is not provided by the facility. It may also be used for back-up
points are available for each cycle, allowing for exposure phase pressure source for the door seal in bioseal applications.
termination based on the calculated F0 value. This is a portable 1.5 Gallon compressor tank that delivers
Bioseal (for AMSCO 250LS double manual door units only) 48 LPM @ 689 KPa (1.7CFM @ 100 PSI). Refer to STERIS
is a 1/4” steel plate which is welded to the chamber and a 1/4” drawing no. 755718-038 for complete specifications.
thick silicone gasket that extends between the plate and a NOTE: UL/CSA certified only.
carbon steel wall frame which is welded to wall imbeds.The Seismic tie-down kit conforms to current California Code of
bioseal is provided on the non-operating end of the sterilizer, Regulations.
prevents passage of airborne microorganisms from the space
between the vessel body and the structural wall opening.Steam PREVENTIVE MAINTENANCE
is the primary source of pressure behind the door seal. All
sterilizers with bioseals have air back-up to maintain seal A global network of skilled service specialists can provide
pressure when out of cycle or if the steam source is not periodic inspections and adjustments to help ensure low-cost,
available. peak performance. STERIS representatives can provide
Air-differential seal (double door units only), provided on information regarding annual maintenance agreements.
the non-operating end of the sterilizer, minimizes airflow
between the dirty and clean sides of the barrier.
Back cabinet panel is provided on single door, freestanding
units where the unit is accessible on all sides.
Air detector (integral factory piping option) is used to
determine whether any air or non-condensible gas present in
the chamber is sufficient to impair the sterilizing process.
Backflow preventer option can be installed on sterilizer piping
to prevent the unwanted reverse flow of water or other
substances into the potable water supply.

6
NOTES Electrical - Controls
120 Volt, 50/60 Hz, 1-phase, 9.5 Amp
1. The sterilizer is not supplied with a vacuum breaker or
International:
backflow preventer and, where required by local codes,
installation of such a device in water line is not provided by 230 Volt, 50/60 Hz, 1-Ph, 1.5 Amp
STERIS. Electrical - Generator
2. Pipe sizes shown indicate terminal outlets only. Building 208 Volt, 50/60 Hz, 3-phase
service lines, not provided by STERIS, must supply the 240 Volt, 50/60 Hz, 3-phase
specified pressures and flow rates.
480 Volt, 50/60 Hz, 3-phase
3. Disconnect switches (with OFF position lockout only; not
600 Volt, 60 Hz, 3-phase
provided by STERIS) should be installed in electric supply
lines near the equipment. International:
4. Access to the recessing area from the control end of the 380/415 Volt, 50/60 Hz, 3-Ph, (Prevacuum Units)
sterilizer is recommended. Sterilizer Feed Water
5. Clearances shown are minimal for installing and servicing 1.0" NPT, 30 to 50 psig (206.8 to 344.7 kPa) dynamic.
the equipment. Refer to Table 1 for water specification guidelines.
6. If loading car and carriage are to be used with a AMSCO Steam Generator Feed Water
250LS sterilizer, front clearance should equal twice the 1/2" NPT, 20 to 50 psig (137.9 to 344.7 kPa) dynamic. Refer
length of the sterilizer. This will permit complete withdrawal to Table 2 for required water quality. Use of feed water within
of the loading car from the chamber and allow convenient the nominal conditions will optimize equipment
maneuverability of the transfer assembly to and from the performance and reduce maintenance.
sterilizer.
7. Floor drain should be provided within confines of sterilizer NOTE: Backflow prevention is not standard on the unit,
framework. but a backflow preventer option can be ordered.

UTILITY REQUIREMENTS Sterilizer Equipped With Integral Stainless-steel

Steam Generator
Refer to Equipment Drawing for complete information.
Sterilizer Feed Water
Sterilizer Using Facility Steam1 1.0" NPT, 30 to 50 psig (206.8 to 344.7 kPa) dynamic.
Steam Refer to Table 1 for required water quality.

1/2" NPT, 50 to 80 psig (344.7 to 551.6 kPa) dynamic, Steam Generator Feed Water
97 to 100% vapor quality 1/2" NPT, 20 to 50 psig (137.9 to 344.7 kPa) Refer to
Drain Table 3 for required water quality.

1-1/2" ODT drain terminal. (Floor drain capacity must handle Drain
peak water consumption; refer to engineering data.) 1-1/2" ODT generator drain terminal. (Floor drain capacity
Electrical - Controls must handle peak water consumption; refer to engineering
data).
120 Volt, 50/60 Hz, 1-phase, 3.0 Amp
Generator Drain
International:
1/2" ODT
230 Volt, 50/60 Hz, 1-Ph, 1.5 Amp
Electrical - Controls
Sterilizer Feed Water
120 Volt, 50/60 Hz, 1-phase, 9.5 Amp
1.0" NPT, 30 to 50 psig (206.8 to 344.7 kPa) dynamic. Water
is used for ejectors, vacuum pumps, exhaust condensers, International:
and trap cooling. Refer to Table 1 for recommended water 230 Volt, 50/60 Hz, 1-Ph, 1.5 Amp
quality. Use of feed water within the nominal conditions will Electrical - Generator
optimize equipment performance and reduce maintenance.
208 Volt, 50/60 Hz, 3-phase
NOTE: Backflow prevention is not standard on the unit, but
240 Volt, 50/60 Hz, 3-phase
a backflow preventer option can be ordered.
480 Volt, 50/60 Hz, 3-phase
600 Volt, 60 Hz, 3-phase
Sterilizer Equipped With Integral Carbon Steel International:
Steam Generator 380/415 Volt, 50/60 Hz, 3-Ph, (Prevacuum Units)
Drain
1-1/2" ODT drain terminal. (Floor drain capacity must handle
peak water consumption; refer to engineering data.)
Generator Drain
1/2" ODT

7
 Refer to the Following Equipment Drawings for Installation Details

Equipment Drawing Number Equipment Drawing Title

AMSCO 110LS
387362-169 110LS, SINGLE DOOR, RECESSED, STEAM HEAT

387362-170 110LS, SINGLE DOOR, CABINET, STEAM HEAT

387362-171 110LS, SINGLE DOOR, RECESSED, ELECTRIC STEAM

387362-172 110LS, SINGLE DOOR, CABINET, ELECTRIC STEAM

387362-173 110LS, DOUBLE DOOR, RECESSED 1 WALL, STEAM HEAT

AMSCO 250LS
387362-159 250LS, SINGLE DOOR, RECESSED, STEAM HEAT

387362-160 250LS, SINGLE DOOR, CABINET, STEAM HEAT

387362-161 250LS, SINGLE DOOR, RECESSED, ELECTRIC STEAM

387362-162 250LS SINGLE DOOR, CABINET, ELECTRIC STEAM

387362-163 250LS, DOUBLE DOOR, RECESSED 1 WALL, STEAM HEAT

387362-164 250LS, DOUBLE DOOR, RECESSED 2 WALLS, STEAM HEAT

387362-165 250LS, DOUBLE DOOR, RECESSED 1 WALL, ELECTRIC STEAM

387362-166 250LS, DOUBLE DOOR, RECESSED 2 WALLS, ELECTRIC STEAM

1. External Supplied Steam (Facility Steam/Stand-Alone Steam Generator)

8
 Table 1. Recommended Feed Water Quality for Sterilizers
Condition Nominal Conditions Maximum Conditions

Temperature 40°-60°F (4°-16°C) 70°F (21°C)

Total Hardness as CaCO3 * 50-120 mg/L 171 mg/L

Total Dissolved Solids 100-200 mg/L 500 mg/L

Total Alkalinity as CaCO3 70-120 mg/L 180 mg/L

pH 6.8-7.5 6.5-8.5

Total Silica 0.1 - 1.0 mg/L 2.5 mg/L

* 17.1 mg/L = 1.0 grain hardness

Table 2. Required Feed Water Quality for Carbon-Steel Steam Generators

Condition Nominal Conditions Maximum Conditions

Temperature 70-140°F (21-60°C) 140°F (60°C)

Total Hardness as CaCO3 * 0-17 mg/L 130 mg/L

Total Dissolved Solids 50-150 mg/L 250 mg/L

Total Alkalinity as CaCO3 50-100 mg/L 180 mg/L

pH 6.8-7.5 6.5-8.5

Total Silica 0.1 - 1.0 mg/L 2.5 mg/L

Resistivity † 2 - 6 k·cm 26 k·cm

* 17.1 mg/L = 1.0 grain hardness

† WARNING-BURN HAZARD: Never use supply water with resistivity

exceeding 26 k·cm, as doing so may cause malfunction of steam genera-
tor level control, resulting in sterilizer operator being severely burned by
scalding water. If supply water resistivity exceeds 26 k·cm immediately
contact STERIS Service Engineering.

Table 3. Required Feed Water Quality Stainless Steel Generators

Type of Water Deionized Water, Distilled or Reverse Osmosis

Temperature 70-140°F (21-60°C)

Minimum Specific Resistivity 1.0 M·cm

NOTE: Do not connect tap water to stainless-steel steam generator. Use of water not
meeting the required feed water quality will invalidate the warranty and is a violation of
ASME Boiler Codes.

9
 ENGINEERING DATA

MAXIMUM OPERATING HEAT LOSSb

WEIGHTa BTU/hr at 70°F (21°C)
Model & lbs (kg) Single Door Double Door
Chamber Size Heating
in (mm) Cab’t Enc Recessed Recessed One Wall Recessed Two Walls
Single Door Double Door To Room Front of Back of Front of Back of At Each Between
Wall Wall Wall Wall End Walls
AMSCO 110LS Steam 750 (340) 989 (449) 4300 1600 2700 1600 3500 N/A N/A
16 x 16 x 26 Electric 890 (404) N/A 6050 2300 3750 N/A N/A N/A N/A
(406 x 406 x 660)
AMSCO 250LS Steam 1231 (558) 1606 (728) 7000 2500 4500 2500 5300 2500 2800
20 x 20 x 38 Electric 1371 (622) 1726 (782) 8750 3300 5450 3300 6250 3300 2950
(508 x 508 x 965)

UTILITIES CONSUMPTION
Waterc
Steam
Model & Cold Hotd
Chamber Size Heating
Peak Per Cyclee Idle Peak Per Idle Peakf Per Cyclee Idle
in (mm)
gpm gal/cycle gph gpm Cyclee gph lb/hr lb/cycle lb/hr
(lpm) (l/cycle) (lph) (lpm) gal/cycle (lph) (kg/hr) (kg/cycle) (kg/h)
(l/cycle)
AMSCO 110LS Steam 6 (23) 68 (257) 7 (27) N/A N/A N/A 180 (81) 18 (8) 7 (3)
16 x 16 x 26 Electric 6 (23) 68 (257) 7 (27) 1 (4) 3 (11) 1 (4) N/A N/A N/A
(406 x 406 x 660)
AMSCO 250LS Steam 6 (23) 70 (265) 10 (38) N/A N/A N/A 180 (81) 21 (10) 7 (3)
20 x 20 x 38 Electric 6 (23) 70 (265) 10 (38) 1 (4) 4 (15) 1 (4) N/A N/A N/A
(508 x 508 x 965)
a Based on chamber fully loaded with water flasks.
Noise level is based on the vacuum pump, 74 dB at 24"Hg vacuum.
Estimated noise level of 68dB for units with water ejector.
b At 70°F (21°C).
c Backflow preventer device in water line, when required by local codes, is not provided by STERIS.
d Hot water recommended for units equipped with electric steam heat.
e Based on Prevac cycle, 3 pulses, 30 minute exposure time and 5 minute dry time. Tested with empty chamber.
f Peak steam demand (lbs/hr) may vary depending on operating conditions.

Additional utilities are required for units with the following options:
• Liquid Air Cool (Compressed Air)
• Decontamination Cycle (Compressed Air)
• Bio-Seal and Air Differential Seal (Optional Compressed Air Backup)
• Vacuum Pump (3-Phase Voltage)
• Stainless-Steel Piped Units (Treated Water)
• Steam Source (one of the following):
>> Integral Steam Generator - Carbon Steel or Stainless Steel (3 Phase Voltage)
>> Indirect Steam Generator; Minimum 75 psig Steam Required

Consult Customer service for specially configured equipment drawings.

10
CUSTOMER IS RESPONSIBLE FOR COMPLIANCE WITH
APPLICABLE LOCAL AND NATIONAL CODES AND
REGULATIONS.

The base language of this document is ENGLISH.

Any translations must be made from the base language
document.

11
For further information, contact:

STERIS Corporation
5960 Heisley Road
Mentor, OH 44060-1834 • USA
440-354-2600 • 800-548-4873
www.STERISLifeSciences.com

This document is intended for the exclusive use of STERIS Customers,

including architects or designers. Reproduction in whole or in part by any
SD1026 ©2019, STERIS Corporation. All rights reserved. Rev B party other than a Customer is prohibited.