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Pollen Tube Growth

The document discusses observing pollen tube growth on a stigma. Pollen grains deposited on a compatible stigma will germinate and produce pollen tubes, which are extensions of the intine that emerge from the pollen grain and pass through the stigma and style tissues to reach the ovule. To view pollen tube growth under a microscope, styles with stigmas from Vinca rosea flowers are softened in boiling water, stained with cotton blue dye, and observed as blue tubular structures moving through the stigma and style tissues.

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513 views2 pages

Pollen Tube Growth

The document discusses observing pollen tube growth on a stigma. Pollen grains deposited on a compatible stigma will germinate and produce pollen tubes, which are extensions of the intine that emerge from the pollen grain and pass through the stigma and style tissues to reach the ovule. To view pollen tube growth under a microscope, styles with stigmas from Vinca rosea flowers are softened in boiling water, stained with cotton blue dye, and observed as blue tubular structures moving through the stigma and style tissues.

Uploaded by

saadathparveen1444
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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EXERCISE

POLLEN TUBE GROWTH ON STIGMA

Aim: To study the pollen tube growth on stigma in a pistil.

Principle: Pollen grains deposited on a compatible stigma germinate and produce the pollen tube. A
pollen tube is an extension of the intine. It emerges through one of the germ pore and passes through
the tissues of stigma and style to reach the ovule. The growing pollen tube is observed by staining the
style and stigma with cotton blue.

Requirement: 2 – 3 excised styles with stigma of Vinca rosea flowers, beaker, water, slides, cover
slips, cotton blue stain, compound microscope, brush and needle.

Procedure:

1. Take a beaker with water and boil.


2. Place the cut styles with stigma in boiling water for 10 minutes to soften the tissues.
3. Take out a stigma and put it on a slide containing a drop of water.
4. Put a drop of cotton blue (Lactophenol) over it and allow it to stand for 2 minutes.
5. Place a cover slip on it and press it gently with your thumb.
6. Observe under low power objective of the compound microscope.

Observation:
Blue coloured tubular structures moving through the tissues of style and stigma are seen.

Pollen grain

Stigma

Style

Pollen tube

POLLEN TUBE GROWTH ON STIGMA

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