Academic Year (2023-2024)

Subject- Chemistry
Chemistry Investigatory Project
Investigatory project on-
Study of acidity of vegetable and fruit
juices
Submitted by: Teacher-in-Charge.
Name: Abdullah Noor Ms. Nadiya
Mrs. Nadiya Anis

Class: XI-C PGT Chemistry

Roll No: 2445 Modern Middle East
International School
 CERTIFICATE
I hereby certify that the project work entitled
"Study of acidity of vegetable and fruit juices" carried th ‘C’
This is to certify that; Abdullah Noor
out to study and investigate about the subjectof class 11
has completed
matter, has been the project work
completed solelyinand
chemistry in the
sincerely by
year 2023-24
Abdullah onpartial
Noor, in “Studyfulfillment
of acidity ofof vegetable and
the Chemistry
fruit juices”
Practical under the- guidance
Examinations of Ms. Nadiya as
AISSCE (2023-2024)
prescribed by CBSE course.
I certify that the project is done by him under direct
supervision and guidance
It is further of the
certified that teacher
this projectas
is per
the the
requirement for the Board Exam
individual work of the student.

Head of Institution Teacher-in-charge

Ms. Shabana Parveen Ms.
Mrs. NadiyaAnis
Nadiya
Principal PGT Chemistry
Modern Middle East Modern Middle East
International School - International School
Riyadh
 Acknowledgement
I would like to express my special thanks of
gratitude to my subject teacher Ms. Nadiya
to give her guidance to make the successful
completion of this project.
I also want to give special thanks to our
principal Ms. Shabana Parveen who gave me
this golden opportunity to do this wonderful
project on the topic “Study of acidity of
vegetable and fruit juices”, so that I will get to
know about detailed information for the same.
Content

• Certificate
• Acknowledgement
• Topic
• Abstract
• Introduction
• Topic related theory and e.g.
• Material required.
• Chemical requirements
• Procedure
• Conclusion
• Bibliography
Abstract
Aim is to analyze some fruits & vegetables juice for
the contents present in them. Fruits and vegetable
are always a part of balanced diet. That means fruits
and vegetables provide our body the essential
nutrients, i.e., Carbohydrates, proteins, vitamins and
minerals.
Again, their presence in these is being indicated by
some of our general observations, like -freshly cut
apples become reddish black after some time.
Explanation for it is that iron present in apple gets
oxidized to iron oxide. So, we can conclude that
fruits and vegetables contain complex organic
compounds, for e.g., anthocin, chlorophyll, esters
(flavoring compounds), carbohydrates, vitamins and
can be tested in any fruits or vegetables by
extracting out its juice and then subtracting it to
various tests which are for detection of different
classes of organic compounds. Detection of minerals
in vegetables or fruits means detection of elements
other than carbon, hydrogen and oxygen.
 Introduction
Vegetable juice (also referred as: Green Drink) is a juice drink
made primarily of blended vegetables and available in the form
of powders. Vegetable juice is often mixed with fruits such
as apples or grapes to improve flavor. It is often touted as a low-
sugar alternative to fruit juice, although some commercial brands
of vegetable juices use fruit juices as sweeteners and may contain
large amounts of sodium.
Our body needs important nutrients to support its healthy
condition. That is why it is imperative that our food consumption
should include healthy foods that contain good amount of
nutrients sufficient to supply our body with its required daily
nutrition. Nutrients like carbohydrates, proteins, fats, vitamins,
minerals, etc. are play vital and specific role to develop and
sustain body.
This project deals with finding out various constituents and
compositions of vegetables and fruits. Analyzing the type of
nutrient present in a food item helps plan a balanced diet. A
balanced diet can be prepared according to the energy
requirement which varies depending on age, sex, size, metabolic
rate and activity level.
 NUTRIENTS

➢ CARBOHYDRATE

The carbohydrates are the optically active

polyhydroxy, aldehydes or ketones or the compounds
which produce such units on hydrolysis.
Carbohydrates are the most common source of energy
in living organisms. Foods high in simple
carbohydrates include fruits, sweets and soft drinks.
Foods high in complex carbohydrates include breads,
pastas, beans, potatoes, bran, rice, and cereals.
Carbohydrates are used as storage molecules as starch
in plants and glycogens in animals.

Role of carbohydrates in body is as follows:

➢ Providing energy and regulation of blood
➢ Biological recognition processes
➢ Dietary fiber
➢ Sparing the use of proteins for energy
➢ Breakdown of fatty acids.
 ➢ PROTEIN

Proteins (also known as polypeptides) are organic

compounds made of amino acids arranged in a linear
chain and folded into a globular form. Proteins are the
basis of many animal body structures (e.g., muscles,
skin, and hair). Each molecule is composed of amino
acids, which are characterized by inclusion of
nitrogen and sometimes Sulphur (these components
are responsible for the distinctive smell of burning
protein, such as the keratin in hair). The body requires
amino acids to produce new proteins (protein
retention) and to replace damaged proteins. Protein
contains 16.8 kilojoules (4 kilocalories) per gram. In
the case of protein, this is somewhat misleading as
only some amino acids are usable for fuel. Chief
sources of protein are milk, pulses, fish, meat, etc.
 ➢FAT

A molecule of dietary fat typically consists of

several fatty acids (containing long chains of carbon
and hydrogen atoms), bonded to a glycerol. They are
typically found as triglycerides (three fatty acids
attached to one glycerol backbone). Fats may be
classified as saturated or unsaturated depending on
the detailed structure of the fatty acids involved. Fats
contain 37.8 kilojoules (9 kilocalories) per gram.

➢ MINERALS
Dietary minerals are the chemical elements required
by living organisms, other than the four
elements carbon, hydrogen, nitrogen,
and oxygen that are present in nearly all organic
molecules.
• Calcium - A common electrolyte, but also
needed structurally (for muscle and digestive system
health, bone strength, some forms neutralize acidity)
may help clear toxins, provides signaling ions for
nerve and membrane functions.
• Magnesium - Required for processing ATP and
related reactions (builds bone, causes strong
peristalsis, increases flexibility, increases alkalinity)
• Phosphorus - Required component of bones;
essential for energy processing prevent anemia.
 CUCUMBER

Cucumber (Cucumis sativus) is a widely cultivated plant in

the gourd family, Cucurbitaceae.Itisacreepingvinethatbears cylindrical fr
uits that are used as culinary vegetables. There are three main varieties of
cucumber: slicing, pickling, and burpless. Within these varieties, several
different cultivars have emerged. The cucumber is originally
from Southern Asia, but now grows on most continents. Many different
varieties are traded on the global market.
The cucumber is a creeping vine that roots in the ground and grows
up trellises or other supporting frames, wrapping around supports with
thin, spiraling tendrils. The fruit of the cucumber is roughly cylindrical,
elongated with tapered ends, and may be as large as 60 centimeters (24 in)
long and 10 centimeters (3.9 in) in diameter. Having an enclosed seed and
developing from a flower, botanically speaking, and cucumbers are
classified as pepos, a type of botanical berry. Much like tomatoes and
squash they are often also perceived, prepared and eaten as vegetables.
Cucumbers are usually more than 90% water.
 CARROT

The Carrot (Daucus carota sativus) is a root vegetable, usually orange in

color, though purple, red, white, and yellow varieties exist. It has a crisp
texture when fresh. The most eaten part of a carrot is a taproot, although
the greens are sometimes eaten as well. It is a domesticated form of
the wild carrot Daucus carota, native to Europe and southwestern Asia.
The domestic carrot has been selectively bred for its greatly enlarged and
more palatable, less woody-textured edible taproot. The Food and
Agriculture Organization of the United Nations (FAO) reports that world
production of carrots and turnips (these plants are combined by the FAO
for reporting purposes) for calendar year 2011 was almost 35.658 million
tons. Almost half were grown in China.
 TOMATO

The tomato (etymology and pronunciation) is the edible, often red fruit/berry of
the nightshade Solanum Lycopersicon, commonly known as a tomato plant. The
tomato is consumed in diverse ways, including raw, as an ingredient in many
dishes, sauces, salads, and drinks. While it is botanically a berry fruit, it is
considered a vegetable for culinary purposes, which has caused some confusion.
The species originated in the South American Andes and its use as a food
originated in Mexico and spread throughout the world following the Spanish
colonization of the Americas. Its many varieties are now widely grown, sometimes
in greenhouses in cooler climates. The plants typically grow to 1–3 meters (3–
10 ft) in height and have a weak stem that often sprawls over the ground and
vines over other plants. It is a perennial in its native habitat, although often grown
outdoors in temperate climates as an annual. An average common tomato weighs
approximately 100 grams (4 oz).
Material Required:
• Test Tubes
• Burner
• Litmus paper
• Laboratory reagents
• Various fruits
• Vegetable juices

Chemical Requirements:
• pH indicator
• Iodine solution
• Fehling solution A and Fehling solution B
• Ammonium chloride solution
• Ammonium hydroxide
• Ammonium oxalate
• Potassium sulphocyanide solution.
Procedure
The vegetable juices are diluted using distilled water. The
distilled water is added to it in order to remove color and
to make it colorless so that color change can be easily
watched and noted down. Now test for food substance is
taken down with the solution.

1. Test for Acidity – Take 5ml of juice in a test tube

and pH values should be noted down by dipping it in the
test-tube. If it turns red, it means that the juice is acidic
else it is basic.
2. Test for Starch – Take 2ml of vegetable juice in a
test tube and add a few drops of iodine solution to it. If
the solution turns blue in color, it indicates the presence
of starch.
3. Test for Proteins – Take 5ml of 5% of NaOH
solution and add 2 drops of CusO4 known as burial
solution and add juice and shake well. If the solution turns
violet in color, it indicates the presence of proteins.
4. Test for Carbohydrates – Take 2ml of Fehling’s
solution A and B and 1ml of Fehling’s solution B in a
Test tube. If the solution turns red it indicates the
presence of sugar like maltose, glucose, fructose and
lactose.
5. Test for Potassium – Add 2ml of juice in a test tube
and picric acid, yellow color precipitate indicates the
presence of potassium.
6. Test for Calcium – Add 2 ml of vegetable juice and
add NH4Cl solution. Filter the solution and to the filtrate
add 2 ml of ammonium oxalate solution. White
precipitate indicates the presence of calcium.
7. Test for Magnesium – Add NH4OH and excess
Disodium Hydrogen Phosphate to test tube with a glass
rod. White precipitate indicates the presence of
magnesium.
Observations
Cucumber
Test Observation Inference
Acid/Base pH Turns Red Acidic
Starch No Blue Color Absence Of Starch
Protein No Violet Color Absence of Protein

Carbohydrate Red Color Presence of Carbohydrate

Potassium No Yellow Precipitate Absence Of Potassium

Calcium White Precipitate Absence Of Calcium

Magnesium White Precipitate Presence Of Magnesium

Carrot
Test Observation Inference
Acid/Base pH turns Red Acidic
Starch No Blue Color Presence Of Starch
Protein No Violet Color Absence of Proteins
Carbohydrate Red Color Presence of
Carbohydrates
Potassium Yellow Precipitate Presence of Potassium
Calcium No White Precipitate Absence of Calcium
Magnesium No White Precipitate Absence of Magnesium
Tomato

Test Observation Inference

Acid/Base pH turns Red Acidic
Starch No Blue Color Presence of Starch
Protein No Violet Color Absence Of Protein
Carbohydrate Red Color Presence of
Carbohydrates
Potassium Yellow Precipitate Presence of Potassium
Calcium White Precipitate Presence of Calcium

Magnesium No White Precipitate Absence of

Magnesium
Result

After performing the tests for carbohydrates,

proteins, fats and minerals, following can be
concluded about their presence in different
vegetables and fruits.
❖ Tomato contains carbohydrates, proteins,
calcium, magnesium.
❖ Cucumber contains carbohydrates, proteins,
fats, calcium, magnesium, iron.
❖ Carbohydrates and starch are rich in tomato
and cucumber.
 ❖ CONCLUSION
After analyzing the vegetables and fruits it can be well
concluded that all of them contain one or the other
compounds vital for body functioning. It is observed that
carbohydrate is a predominant constituent while fats are
not present in most of the tested items. It is a natural merit,
as living organism require carbohydrate the most common
for generating energy. Among minerals, presence of
calcium is considerable as its present in all the selected
food items. Iron, magnesium and phosphorous are also
present sufficiently. Many other minerals form constituents
of vegetables and fruits, but in trace quantities as body
require them very less.
The results throw a light on significance of vegetables and
fruits as their constituents are vital compounds and
nutrients. The deficiency of these nutrients can lead to
various metabolic disorders. So, besides cereals, milk and
its products and non-vegetarian food items, vegetable and
fruits must be included in a daily balanced diet of all. More
effort is required to make everyone realizes their
significance for a healthy, disease-free, long lifestyle.
Bibliography

➢ www.academia.edu
➢ NCERT Textbook
➢ Wikipedia
➢ www.seminarsonly.com