C146-E188C

Liquid Chromatograph Mass Spectrometer

LCMS-8040
 Ultra-High Sensitivity
By incorporating newly improved ion optics and collision cell technology, the LCMS-8040
provides higher multiple reaction monitoring (MRM) sensitivity. A five-fold increase in sensitivity
(reserpine, S/N ratio) has been achieved by improving ion focusing and minimizing ion losses
between multi-pole lenses. These improvements also yield higher sensitivity for scan mode
measurements. This higher sensitivity expands the potential range of LC/MS/MS applications.

Ultra-High Speed
The LCMS-8040 was designed to provide significantly higher sensitivity while maintaining the
high speed offered by the LCMS-8030. Ultrafast MRM transition speeds, up to 555 MRMs per
second, are achieved by Shimadzu’s UFsweeper collision cell technology, proprietary
high-precision quadrupole machining capabilities, and unique high voltage power supply
technology. In addition, the LCMS-8040 features the world’s fastest * polarity switching at
15 msec. With this high-speed performance, the LCMS-8040 can dramatically improve
analytical throughput.

Ultra-High Reliability
MRM optimization in Shimadzu’s LCMS systems is based on a rapid series of automated flow
injection analyses, requiring only minutes to perform. Multiple compounds can be optimized
in an unattended sequence, freeing the analyst from tedious work. MRM parameters
optimized for the LCMS-8030 can be transferred to the LCMS-8040, making it possible to
transfer methods between systems. The LCMS-8040 offers the same ease of maintenance
benefits as the LCMS-8030, and all consumables, such as desolvation lines (DL) and ESI
capillaries, are interchangeable as well.

* Per survey result as of May 2012

GCMS-QP2010 Ultra GCMS-TQ8030 LCMS-8030 LCMS-8040 LCMS-8050 LCMS-2020 LCMS-IT-TOF iMScope MALDI-7090
GCMS-QP2010 SE
 Ultra-High Sensitivity with UFMS Technology

Higher Sensitivity with Improved Ion Optics

The UF-Lens is a lens system that offers both higher sensitivity and easier maintenance. The optical system
TM
UF-Lens integrates two multi-pole RF ion guides for higher sensitivity. In addition, the lens system can be removed
without tools for easy cleaning.

Sprayer UF-Lens™
Qarray™ Multi Pole 1 Q1 Pre-rod Q1 Post-rod Q3 Pre-rod
Q1 Q3

Desolvation
Line Skimmer Multi Pole 2 UFsweeper™ Ⅱ Detector
Collision Cell
Atmospheric 1st Vacuum
2nd 3rd 4 th
Pressure Chamber

RP Triple Inlets Tmp 4 Stage Differential Pumping System

Ion losses between segments are minimized by

Previous UF-Lens
utilizing quadrupole ion guides. Configuration

LCMS-8030 LCMS-8040

5
A comparison of auto-tune Q1 scan results for a standard sample (a mixture
Increase in Sensitivity (8030 vs. 8040)

of PEG, PPG, and raffinose) obtained from the LCMS-8030 and LCMS-8040 is
4
shown to the right. Target ions used for ESI + auto-tuning are plotted (m/z
65.05, 168.10, 256.15, 344.20, 652.40, 1004.60, and 1224.75). A significant
3
sensitivity increase across a broad molecular weight range for precursor ion
transmission is demonstrated.
2

0
0 500 1000 1500 2000
m/z

4 Speed Beyond Comparison Enhanced Sensitivity

 Higher CID Efficiency with Improved Collision Cell
TM
UFsweeper
The UFsweeper II is a high-sensitivity, high-speed collision cell that
features improved ion focusing by using high-speed ion transport
technology. This yields better product ion transmission in the
collision cell, maintaining signal intensity and suppressing crosstalk,
even for high-speed or simultaneous multi-component analysis. The
capability for high-throughput analysis is thus maintained at lower
levels of detection.

Precursor Product LCMS-8030 LCMS-8040 Proportional

Sample Name
m/z m/z CID efficiency CID efficiency Increase
Lidocaine 235.4 86.1 20.0 29.0 1.4
Atropine 290.1 124.1 7.2 11.0 1.5
Yohimbine 355.1 144.0 9.5 15.6 1.6
Tetracaine 265.1 176.1 15.7 32.0 2.0
Doxepin 280.1 107.1 4.4 7.2 1.6
Imipramine 281.1 86.1 16.2 22.1 1.3
Nortriptyline 264.1 233.1 3.8 7.4 1.9
Isopropylantipyrine 231.1 188.9 1.5 2.9 1.9
Diazepam 285.0 154.0 2.4 3.9 1.6
Reserpine 609.3 195.1 3.9 6.6 1.7

The LCMS-8040 offers higher sensitivity while maintaining the measured simultaneously, both the LCMS-8030 and LCMS-8040
ultrafast performance of the LCMS-8030. The figures below show accurately identified all 167 components. In addition, the LCMS-8040
simultaneous analysis of 167 pesticides in MRM positive /negative showed improved sensitivity – an average of three times higher for
ion analysis mode. Even though positive and negative ions were all components.

LCMS-8030 LCMS-8040
(×10,000,000) (×10,000,000)
1.00 1.00

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
5.0 10.0 15.0 20.0 min 5.0 10.0 15.0 20.0 min

HPLC MS
Analysis Column Shim-pack FC-ODS (2.0 mmI.D. × 150 mmL., 3µm) Probe Voltage +4.5 kV (ESI-Positive mode) / −3.5 kV (ESI-Negative mode)
Mobile Phase A 5 mmol/L ammonium acetate - Water Nebulizing Gas Flow 1.5 L /min
Mobile Phase B 5 mmol/L ammonium acetate - Methanol Drying Gas Flow 10 L/min
Gradient Program 15 %B (0 min) – 40 %B (1–3.5 min) – 50 %B (6 min) DL Temperature 250 °C
– 55 %B (8 min) – 95 %B (17.5–30 min) – 15 %B (30.01–40 min) Heat Block Temperature 400 °C
Flow Rate 0.2 mL/min
Column Temperature 40 °C

Liquid Chromatograph Mass Spectrometer 5

 Proven Ultra-High Performance now
with Enhanced Sensitivity
Higher Sensitivity for Q1 Full Scan Spectra
The LCMS-8040 maintains the same high-speed scanning (UFscanning) and polarity switching
technology (UFswitching) utilized in the LCMS-8030.
The LCMS-8040 not only maintains Shimadzu's proprietary pesticide samples (Methomyl, Carbaryl (NAC), Phoxim, Benfuracarb,
high-speed technologies (UF Technologies, USP7855355, USP8188426), and Abamectin B1a) is shown below. The upper spectra were
which minimize sensitivity losses even at faster scan speeds, it also acquired using the LCMS-8040 and the lower spectra were acquired
features improved ion optics, which provide higher sensitivity for using the LCMS-8030. As shown, the LCMS-8040 offers significant
MRMs and full scans. A comparison of Q1 full scan spectra for sensitivity improvements for precursor ions or full scan data.

LCMS-8040
Inten.(×10,000,000) Inten.(×10,000,000) Inten.(×10,000,000)
2.0 2.0 2.0
×4 ×3 ×3
×2 ×2 ×2
1.5 1.5 1.5
×3 ×3
×3 ×3
1.0 ×3 1.0 ×3 1.0

×5 ×5
0.5 ×5 0.5 0.5

200 300 400 500 600 m/z 200 300 400 500 600 m/z 200 300 400 500 600 m/z
15000 u/sec 10000 u/sec 5000 u/sec

LCMS-8030
Inten.(×10,000,000) Inten.(×10,000,000) Inten.(×10,000,000)
2.0 2.0 2.0

1.5 1.5 1.5

1.0 1.0 1.0

0.5 0.5 0.5

200 300 400 500 600 m/z 200 300 400 500 600 m/z 200 300 400 500 600 m/z
15000 u/sec 10000 u/sec 5000 u/sec

Proven Interface for Robust Performance

Robust, User-Friendly Interface
The LCMS-8030 and LCMS-8040 use the same interface. In LCMS
analysis, it is necessary to adjust interface conditions such as
temperatures and gas flow rates for optimal desolvation. Spray
needle adjustment and probe position are also factors to be
Central position −2mm +3mm Integrated
evaluated. In both the LCMS-8030 and LCMS-8040, temperatures
Locking screw ESI Capillary
and flows are easily controlled through the software. ESI probe
position is set by a single easily accessible knob, and spray needle Position
adjustment knob
protrusion is adjusted without the need for any special tools or
disassembly of the probe. In addition, this capillary incorporates a
Attach/detach with
tapered design, which reduces sample clogging.
a single flip of a lever

6 Speed Beyond Comparison Enhanced Sensitivity

 Higher Sensitivity for MS/MS Acquisition Modes
The increased scan sensitivity of the LCMS-8040 also applies to Mass error
300.0
scan modes specific to triple quadrupole mass spectrometers, such associated with a
rise in scan speed
as product ion scanning, precursor ion scanning, and neutral loss
scanning. Historically, mass spectrometers have introduced mass

Increase in Scan Speed

deviation in linked scans, such as precursor ion scans or neutral loss 300.5
scans, when measured at maximum scan speeds.

Precursor ion of m/z 149 Dipropyl phthalate

Inten.(×10 5) Inten.(×10 5) 301.0
251.25 7.5 251.25
CH3
5.0
5.0

2.5 CH3 298.0 299.0 300.0 301.0 302.0 303.0 m/z

2.5
m/z 149
0.0 0.0 Typical triple quadrupole mass spectrometer
240.0 250.0 m/z 240.0 250.0 m/z
Inten.(×10 7 ) Inten.(×10 7 )
2.0
2727 u/sec 6000 u/sec
8040 However, Shimadzu's proprietary UFscanning technology allows
1.0 1.5
performing precursor ion scans or neutral loss scans at high speeds
8030
1.0 without loss of mass accuracy. In addition, the LCMS-8040 offers
0.5 higher sensitivity levels. Precursor scan results for eight kinds of
0.5 phthalate esters are shown to the left. Scans were performed at
0 0
two speeds, 2727 u /sec and 6000 u /sec. No mass shift is observed
3.0 4.0 5.0 min 3.0 4.0 5.0 min at either scan speed, and a significant sensitivity improvement is
Precursor Ion Scan of Standard Mixture Sample of 8 Phthalate Esters observed for the LCMS-8040.

Easy Method Transfer

MRM parameters for the LCMS-8030 can be used in LCMS-8040 systems.
Optimization of MRM parameters is an early step in LCMS method employing LCMS-8030 MRM conditions will be able to transfer
development. Shimadzu has streamlined the MRM optimization these MRMs directly to the LCMS-8040. To demonstrate this, a
process with a rapid and simplified approach based on automated method for the simultaneous analysis of 167 pesticides was
flow injection analysis. Several method packages, which contain transferred without modification from the LCMS-8030 to the
chromatographic and optimized MRM conditions for a variety of LCMS-8040. Increased sensitivity was obtained for all compounds;
analytes, including residual pesticides, veterinary drugs, and three example chromatograms are shown below.
forensic drugs of abuse, have also been released. Laboratories

10 ppb: Cloquintocet-mexyl(9.5X) 10 ppb: Pyrazosulfuron-ethyl(7.4X) 10 ppb: Linuron(5.2X)

(×100,000) (×100) (×1,000)
1.50 ESI+ 336.20 >238.00 ESI+ 415.20 >182.10 ESI 246.90 >160.00
1.25
1.25 8040 8040 1.50 8040
1.00
1.00
0.75 1.00
0.75
0.50
0.50 0.50
0.25 8030 8030
0.25
8030
0.00 0.00
0.00
18.0 19.0 min 5.5 6.0 6.5 7.0 min 14.0 14.5 15.0 min

Liquid Chromatograph Mass Spectrometer 7

 Ultra-High Reliability to
Withstand Wide Range of Complex Matrices
Exceptional Durability
Blood plasma samples were spiked with verapamil and warfarin, and warfarin by ESI − was performed. Chromatograms for the 1st, 250th,
then deproteinized according to the pretreatment process indicated and 450th measurements are shown below. This resulted in 1 pg
below. The area values from 450 consecutive LCMS-8040 analyses on-column area repeatability of 4.18 % for verapamil and 6.61 %
were then plotted. Simultaneous analysis of verapamil by ESI + and for warfarin.

×10 4
3.0 Column : Shim-pack XR-ODS II
Verapamil (2.0 mmI.D. × 50 mmL, 2.2 µm)
2.5 Mobile Phase A: 5 mmol/L
Warfarin
ammonium acetate –water
2.0 Mobile Phase B: Acetonitrile
Gradient Program:
Area

1.5 60 % B (0 –1.50 min) –

90 % B (1.51– 3.00 min) –
1.0 60 % B (3.01– 4.50 min)
Flow Rate : 0.4 mL /min.
0.5

0.0
0 50 100 150 200 250 300 350 400 450
Injection
200 µL plasma sample
Add 200 µL Acetonitrile, 50 µL 50 % methanol aqueous solution and 50 µL verapamil and warfarin standard solutions.
Vortex and centrifuge (10000 rpm, 3 min.) followed by freeze drying.
Add 500 µL dilution solution.
Vortex and centrifuge (12000 rpm, 5 min.).

(×1,000) (×1,000) (×1,000)

6.0 1st injection 6.0 250 th injection 6.0 450 th injection

5.0 5.0 5.0
4.0 4.0 4.0
3.0 3.0 3.0
2.0 2.0 2.0
1.0 1.0 1.0
0.0 0.0 0.0

0.3 0.4 0.5 0.6 0.7 min 0.3 0.4 0.5 0.6 0.7 min 0.3 0.4 0.5 0.6 0.7 min

Ultra Fast Speed Combined with Lower Femtogram Detection

Using the same chromatography parameters as described above, analysis Verapamil Warfarin
of plasma samples spiked with 40 fg /µL verapamil and warfarin were 300 307.20 >161.05 (-)
1200 455.25 >165.05 (+)
injected into the LCMS-8040 for signal to noise determination. For 100
fg on-column, a S/ N ratio of 146 (rms) was obtained for verapamil and 250
1000
30 (rms) for warfarin.
800 200

600 150
The resulting lower limits of detection for S/ N = 3 (rms) were 2.05 fg for
verapamil and 9.88 fg for warfarin. 100
400

Compounds Verapamil Warfarin 200 50

S/N : 100 fg on column (rms) 146 30 0 0

0.25 0.50 min 0.50 0.75 1.00 min

LOD (fg) Calculated at S/N = 3 2.05 9.88
Chromatograms of 100 fg Verapamil and Warfarin On-Column

8 Speed Beyond Comparison Enhanced Sensitivity

Single Vendor Solution Provides Seamless Operation
LabSolutions LCMS Version 5
LabSolutions LCMS Version 5 is integrated workstation software used The Data Browser allows the user to analyze and compare multiple
to control LCMS-2020 /8030 /8040 systems. Full HPLC control is sets of data, such as peak detection data or LCMS spectra in the
included for LC-VP and Prominence modules, and for the Nexera same window. It also allows LC and MS data to be linked, which is
UHPLC series. Standard data processing features include a especially useful when searching for or identifying compounds. In
Quantitation Browser, which is useful for quantitative processing of addition, the popular report function was expanded to enable
multiple analytes, and a Data Browser for overlaying chromatograms. printing results in a wider variety of formats.

Automatic MRM Optimization

Optimizing MRM parameters is an impor tant step when creating quantitative methods for triple quadrupole mass spectrometers.
LCMS - 8030 / 8040 systems optimize MRM parameters using automated flow injection analysis, an optimization function included
LabSolutions that allows anyone to optimize parameters easily.

Entering precursor ions

Selecting parameters to optimize

Unknown product ions

Optimization choices include voltages for Q1 pre-rod, Q3

pre-rod, and Collision Energy. CE optimization finds the
optimal value by first searching a wide range at 5 volt
steps, then searching again within a ±5 V range around
Optimization results the highest intensity value at 1 volt steps. MRM transitions
are determined by selecting the product ions with the
highest abundance. This entire series of measurements is
performed automatically and a number of analytes can be
optimized in an unattended batch run.

Optimization results are automatically reflected

in method files, and shown in the window
above. Values indicated as "Selected" are the
selected product ions. The user can easily make If a product ion is unknown, the software
edits to the final method. automatically searches for the ion.

Liquid Chromatograph Mass Spectrometer 9

 Powerful Workflow Solutions

When performing quantitation, system parameters must first be optimized. Shimadzu offers
various method packages to eliminate the need for MRM determination and chromatographic
method development.

Residual Pesticides Veterinary Drugs Water Quality Analysis

No. of compounds: 167 No. of compounds: 42 No. of compounds: 44 (Golf Course Pesticides),
32 (Pesticides Targeted for Water Quality Control)

Drugs of Abuse Rapid Toxicology Lipid Mediators Primary Metabolites

No. of compounds: 286 Screening System No. of compounds: 130 No. of compounds: 55
No. of compounds: 106

Quan Solution Software

Optional Open Solution Series Software
Open Access Sample Logging for Triple Quadrupole LCMS
Login Sample Vial Selection
With sample logging now made easy, Quan
Solution allows users unfamiliar with LCMS
software the capability to perform
LC/MS/MS analysis using pre-set methods.
Simple screens allow users to access the
power of a triple quadrupole LCMS without
extensive training.

Sample registration by selecting

the standard samples, unknown
samples, and QC samples at the
specified positions
Quantitative Reports Received Via E-mail

Analysis

10 Speed Beyond Comparison Enhanced Sensitivity

 Profiling Solution Version 1.1
Data Processing Software for Profiling
Profiling Solution software analyzes a huge amount of data from chromatograms, extracting every peak from multiple data files and creating
a data table, which is required for multivariate analysis. Profiling Solution supports both LC/MS and GC/MS instruments and allows
multivariate analysis with various analytical methods.

Data file
instrument
Analytical
Multiple data

Detected Peak
Data table
analysis software
Multivariate

Optional Ion Source

Changing between ESI, APCI and DUIS interface is a completely tool-free operation.

APCI DUIS
Sample injection
ESI probe

Corona needle
Corona needle

Liquid Chromatograph Mass Spectrometer 11

 LCMS-8040

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