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Data Test Revision Sheet Biology QCAA YR 11 Unit 1

The document provides information and examples about calculating total magnification using objective lens and eyepiece magnification. It also discusses calculating field of view under different magnifications and provides sample questions to practice these calculations.

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0% found this document useful (0 votes)
765 views6 pages

Data Test Revision Sheet Biology QCAA YR 11 Unit 1

The document provides information and examples about calculating total magnification using objective lens and eyepiece magnification. It also discusses calculating field of view under different magnifications and provides sample questions to practice these calculations.

Uploaded by

jainkuvam
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Magnification

TO CALCULATE TOTAL MAGNIFICAITON

Total = eye piece magnification x objective lens magnification.

Example : 4 x 10
= 40 X

TO CALCULATE FIELD OF VIEW (FOV)


There are two ways you could use:
Ma High power/low power
Db = x Da
Mb
O. g . magnification For example, we had a FOV of 4.5mm when
New FOV = x O.g. FOV
New magnification using the 40 X magnification.

To calculate for 100 X magnification:


For example, we had a FOV of 4.5mm when = High power/low power
using the 40 X magnification. = 100/40
To calculate for 100 X magnification: = 2.5 more magnified compared to original.
40 Therefore, the new FOV would be decreased
New FOV = x 4.5
100 by 2.5 times.

New FOV = 1.8 mm New field of view = 4.5mm/2.5


= 1.8 mm
Questions to practice:
1. A year 11 student used a microscope with an eyepiece magnification
of x10. The field of view was measured using a ruler under the
microscope with an objective lens of X4.
a) Determine the field of view for the magnification of X4.
b) Calculate the field of view for a magnification of X10.
c) Determine the total magnification.

2. Cheeks cells were studied in a lab. The eye piece magnification was
X10 and the field of view on the lowest magnification X40 was 3.2mm
or 3200 micrometres.

a) Calculate the field of view for a magnification of X200.


b) Determine the objective lens magnification of the X100
magnification.

3. A teacher used a microscope with an eyepiece magnification of x4.


The field of view was measured using a ruler under the microscope
with an objective lens of X10.

a) Determine the total magnification.


b) Calculate the FOV when the objective lens is changed to X40.
Elodea

A group of year 11 students put Bromothymol blue indicator in four test tubes. A student labelled
four test tubes A, B, C, D. Elodea was put in test tubes A and B. A student put test tubes A and C in
front of a light and test tubes B and D in the cupboard.

1. Complete the observation table:

Test tubes in light Test tubes in dark


A B C D
Initial Blue Blue Blue Blue
colour
Final
colour

2. Identify the reason for the colour change in test tube B.


3. Identify the biochemical pathway responsible for the colour to be blue.
4. Deduce the pH change when the indicator turns blue.
5. Identify the biochemical pathway responsible for the colour to be yellow.
6. Deduce the pH change when the indicator turns yellow.
7. Draw conclusions to the change in colour of the indicator if a student was to blow into it with a
straw.

Algae balls

Algae balls were placed at different distances from a light source in a solution with high
concentration of carbon dioxide gas. The following data was collected.
Distance from Number of pH initial pH final
light source algae balls
(cm)
10 10 5.7 6.2
10 50 5.7 7.2
30 10 5.7 5.8
30 50 5.7 6.0

1. Identify the relationship between pH and the distance from the light source.
2. Identify the relationship between pH and the number of algae balls from the light source.
3. Deduce the biochemical pathway responsible for the colour change of the indicator.
4. Draw conclusions about the pH of the algae balls that have no light source.
5. Draw conclusions to the change in pH for the algae balls placed at 30cm.
Osmosis
A group of Year 11 students conducted an osmosis experiment in class using potato cores. They
created a graph using their data collected.

1. Identify the potato cores which increased in mass.


2. Determine the % change in mass for the potato core placed into the 0.6M starch solution.
3. Predict the change in mass for a potato core placed into a 0.3M starch solution.
4. Justify what the concentration of the starch solution would be, when the potato core mass
would not change.

An experiment was set up using dialysis tubing. Inside the dialysis tubing was filled with a starch
solution and the surrounding solution was distilled water.

1. Predict the volume of water inside the dialysis tubing at the end of the A1/A2 experiment.

2. Predict the volume of water inside the dialysis tubing at the end of the B1/B2 experiment.

3. Explain the movement of water molecules during Osmosis.


Enzymes
Enzymes are proteins which catalyse only one type of reaction. The rate at which an enzyme-
catalysed reaction takes place is dependent on a number of factors. The figures below show an
enzyme reaction rate at different pH and temperatures.

Figure 1: Rate of reaction for pepsin, salivary amylase, and trypsin.

1. Identify the pH conditions which Pepsin (enzyme) will work at the optimum rate.
2. Identify the pH conditions which Salivary amylase (enzyme) will work at the optimum rate.
3. Identify the pH conditions which Trypsin (enzyme) will work at the optimum rate.
4. Predict the rate of enzyme activity if Salivary amylase (enzyme) is placed in a pH of 7.2.
Calculating cell SA:V

1. Calculate the surface area, volume, and SA:V ratio for each cell.
1 µm 2 µm 4 µm
Surface area
Volume
SA:V ratio

2. Using data from the table, identify the relationship between SA:V and the size of the cell.
3. Deduce which cell would be most efficient at gaining nutrients via diffusion.

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