Agilent 1290 Infinity II Vialsampler

Data Sheet
Agilent 1290 Infinity II Vialsampler Data Sheet

Product Description
The Agilent 1290 Infinity II Vialsampler is designed for UHPLC applications up to
1300 bar. It provides the reliability, safety, and ease-of-use needed for routine
pharmaceutical tasks and quality control, as well as for environmental and food
analyses. It can house optionally the integrated column compartment for two LC
columns with temperature control up to 80 °C as well as a sample cooler for
stable temperatures down to 4 °C, all within one module.

Features
• Accurate and precise injections within a wide and flexible range of volumes
• Capacity for up to 132 vials (2 mL) or up to 36 vials (6 mL)
• Easy adaption for injection volumes up to 1500 µL for applications ranging
from microbore to semipreparative chromatography
• Includes a needle flush port for rinsing of outside needle to maintain lowest
carryover during routine work
• Integrated column compartment as option or upgrade available, holding two
columns up to 30 cm length, and providing heating capacity up to 80 °C for
reproducible chromatography data at optimized resolution
• Low internal volume for the minimum contribution to a system's total internal
volume, which can be even further reduced using "bypass" mode
• Overlapped injections for increased productivity
• Injection program available for customizing advanced injections as well as for
sample preparation steps upfront injection

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 Specifications
Table 1 Physical Specifications

Type Specification Comments

Weight 19 kg (41.9 lbs) w/o sample cooler

Dimensions 324 x 396 x 468 mm

(height × width × depth) (12.8 x 15.6 x 18.4 inches)

Line voltage 100 – 240 V~, ± 10 % Wide-ranging

capability
Line frequency 50 or 60 Hz, ± 5 %

Power consumption 350 VA / 350 W / 1195 BTU/h

Ambient operating 4 - 40 °C (39 - 104 °F), without chiller

temperature up to 55 °C (131 °F)

Ambient non-operating -40 – 70 °C (-40 – 158 °F)

temperature
Humidity < 95 % r.h. at 40 °C (104 °F)1 Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Non-operating altitude Up to 4600 m (15092 ft) For storing the

module
Safety standards: IEC, Installation category II, Pollution For indoor use only.
CSA, UL degree 2

ISM Classification ISM Group 1 Class B According to CISPR

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1
If a sample cooler is included the upper value for humidity can be reduced. Please check
your lab conditions to stay beyond dew point values for non–condensing operation.

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Table 2 Performance Specifications (G7129B)

Type Specification Comment

Injection range 0.1 – 20 µL in 0.1 µL increments Up to 1500 µL with

(default) 1400 µL-multi-draw kit and
0.1 – 40 µL in 0.1 µL increments if 100 µL-analytical head
40 µL loop is installed
0.1 – 120 µL in 0.1 µL increments up to 130 MPa (1300 bar,
with 1290 Infinity large volume 18854 psi)
injection kit (hardware
modification required)
0.1 – 100 µL in 0.1 µL (if up to 60 MPa (600 bar, 8702 psi)
100 µL-loop and 100 µL-head is
installed)

Precision <0.25 % RSD of peak areas from Measured caffeine

5 µL to 100 µL

Pressure range Up to 130 MPa (1300 bar,

18854 psi)
Sample 0.2 – 5 cp
viscosity range

Sample 132 x 2 mL vial (two trays default)

capacity 100 x 2 mL vial (two classic trays
optional)
36 x 6 mL vials (two trays optional)

Carry over <0.004 % (40 ppm) with needle Using the following conditions:
wash • ZORBAX Eclipse Plus C18,
RRHD, 2.1 x 50 mm, 1.8 µm
(959757-902)
• Mobile Phase:
• A: 0.1 % TFA in water
• B: 0.1 % TFA in acetonitrile
• Isocratic : % B=33 %
• Flow rate: 0.5 mL/min
• Column temperature: 50 °C
• Wavelength detection:
257/4 nm, ref. wavelength
380/100 nm, 20 Hz
• Injection volume: 1 µL
• Sample: 1200 ng/µL
Chlorhexidine for UV,
(dissolved with mobile phase
A), 1 µL injected and measured
both on Agilent G7117B DAD
• Wash solution: H2O with 0.1 %
TFA (5 s)
Injection cycle 18 s for draw speed 200 µL/min
time Ejection speed: 200 µL/min
Injection volume: 1 µL

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 Table 2 Performance Specifications (G7129B)

Type Specification Comment

Minimum 1 µL from 5 µL sample in 100 µL Needle height offset has to be

sample volume microvial, or 1 µL from 10 µL adapted to ensure that needle
sample in 300 µL microvial. doesn’t touch vial bottom. Default
needle height = 0 equates to 2 mm
above the vial bottom.
Control and Agilent Open Lab CDS A.02.02 or above
data evaluation Mass hunter QQQ B.08.01 or above
Mass hunter TOF/QTOF B.07.02 or above
Lab Advisor B.02.07 or above
ICF for 3rd party SW control A.02.04 or above
LC and CE Drivers A.02.12 or above

Local control Agilent Instant Pilot (G4208A) B.02.17 or above

Communications Controller-area network

(CAN),Local Area Network (LAN)
ERI: ready, start, stop and
shut-down signals

Safety and Extensive support for

maintenance troubleshooting and maintenance
is provided by the Instant Pilot,
Agilent Lab Advisor, and the
Chromatography Data System.
Safety-related features are leak
detection, safe leak handling, leak
output signal for shutdown of
pumping system, and low voltages
in major maintenance areas.
GLP features Early maintenance feedback (EMF)
for continuous tracking of
instrument usage with
user-settable limits and feedback
messages. Electronic records of
maintenance and errors.
Housing All materials recyclable.

Metering Metering device in high pressure

device flow path

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Ordering Details
Table 3 1290 Infinity II Vialsampler Ordering Details

Description Part Number

Agilent 1290 Infinity II Vialsampler G7129B

UHPLC design autosampler up to 1300 bar for 0.1 – 20 µL
injections. Includes two drawers, each 66 sample vials (2 mL)
132 vials total capacity, standard needle flush port and
peristaltic pump. Default setup with 20 µL loop and 40 µL
analytical head.
Agilent Infinity II Sample Cooler G7129B #100
Agilent Infinity II Sample Cooler is a cooling unit to fit
G7167A/B samplers as well as G7129A/B samplers. Slide-in
device, customer installable.

Integrated column compartment, 3.0 µL heater G7129B #063

Integrated column compartment for up to two columns with
3 µL heater volume, for standard analytical flow rates up to
5 mL/min.
Recommended for standard flow rates.

Integrated column compartment, 6.0 µL heater G7129B #066

Integrated column compartment for up to two columns with
6 µL heater volume, for standard analytical flow rates up to
5 mL/min
Recommended for high flow rates.

6 mL vial drawer (3x6 positions), 1 drawer/pack G7129B #011

Each drawer can hold 18 x 6 mL vials. Samples are set up in a
A1 to C6 format (microtiter-plate style).
Classic drawer kit (10x10 positions split in 2 drawers) G7129B #012
Kit of 2 drawers to hold 100x 2 mL vials. Samples are set up in a
1-to-100 format (classic Agilent vial sampler style)
Analytical head 100 µL G7129B #161
100 µL analytical head for use at up to 600 bar system pressure
Sample loop 40 µL G7129B #150
Sample loop 40 µL for max 40 µL injection

Sample loop 100 µL G7129B #152

Sample loop 100 µL for max 100 µL injection (600 bar)

Multidraw option adding 80 µL G7129B #022

Includes seat capillary to inject up to 120 µL volume.

Walkup extension: external tray G7129-60000

External tray offering 5 vial positions (2 mL) and 1 vial disposal
position
Walkup extension: vial disposal tube G1313-27302
Disposal tube to fit on vial disposal position

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 www.agilent.com/chem/1200
This information is subject to change without notice.

© Agilent Technologies, Inc., 2015

Published September 1, 2015
5991-6286EN

Agilent Technologies