Lumina Fluorescence Spectrometer

Service Manual
© 2009 Thermo Fisher Scientific Inc. All rights reserved.

Thermo Fisher Scientific Inc. provides this document to its customers with a
product purchase to use in the product operation. This document is copyright
protected and any reproduction of the whole or any part of this document is
strictly prohibited, except with the written authorization of Thermo Fisher
Scientific Inc.

The contents of this document are subject to change without notice. All technical
information in this document is for reference purposes only. System
configurations and specifications in this document supersede all previous
information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is

complete, accurate or error-free and assumes no responsibility and will not be
liable for any errors, omissions, damage or loss that might result from any use of
this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific
Inc. and a purchaser. This document shall in no way govern or modify any
Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern
all conflicting information between the two documents.

For Technical Support, please contact:

Thermo Fisher Scientific
5225 Verona Road
Madison, WI 53711-4495 U.S.A.
Telephone: 1 800 532 4752
E-mail: us.techsupport.analyze@thermofisher.com
World Wide Web: http://www.thermo.com/spectroscopy

For International Support, please contact:

Thermo Fisher Scientific
Telephone: +1 608 273 5017
E-mail: support.madison@thermofisher.com
World Wide Web: http://www.thermo.com/spectroscopy

Microsoft, Windows, Windows NT and Excel are either trademarks or registered

trademarks of Microsoft Corporation in the United States and/or other countries.
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Systems, Incorporated in the United States and/or other countries. InstallShield is
a trademark or registered trademark of InstallShield Software Corporation in the
United States and/or other countries. All other trademarks are the property of
Thermo Fisher Scientific Inc. and its subsidiaries.
For Safety

The following safety symbols are uses on this product.

Always refer to the system manual when working near locations

at which the alert mark shown on the left is attached. If the
operation, etc., is performed without heeding the advice in the
System manual, there is a risk of personal injury. In addition, the
equipment performance may be reduced. Moreover, this alert
mark is sometimes used with other marks and descriptions
indicating other dangers.

When supplying power to this equipment, connect the

accessory 3-pin power cord to a 3-pin grounded power outlet. If
a grounded 3-pin outlet is not available, use a conversion
adapter and ground the green wire. If power is supplied without
grounding the equipment, there is a risk of receiving a fatal
electric shock and equipment damage.

‘On’ (power)

To indicate connection to the mains, at least for mains

switches or their positions, and all those cases where
safety is involves

‘OFF’ (power)

To indicate disconnection from the mains, at least for

mains switches or their positions, and all those case
where safety is involves.

The user cannot repair this equipment.

DO NOT attempt to open the case or to disassemble internal
parts.
Only Thermo Scientific trained service personnel or staff from
Repair your sales representative with knowledge of electrical fire and
Warning shock hazard should service this equipment.
 There are high-voltage parts in this equipment presenting a risk
of severe injury or fatal electric shock to untrained personnel.
In addition, there is a risk of damage to precision parts.

This equipment should be used in the correct position. If the

equipment is turned on its side, etc., it will be unstable and may
Falling Over
be damaged if it falls over as a result of receiving a slight
mechanical shock.

Before changing the fuses, ALWAYS remove the power cord

from the power-outlet and replace the blown fuses. ALWAYS
Changing Fuse
use new fuses of the type and rating specified on the fuse
Warning marking on the rear panel cabinet.
AC 250V T2AL/T5AL indicates a time-large fuse. There is risk of
receiving a fatal electric shock if the fuses are replaced with the
power cord connected.

Keep the equipment free of dust.

Clean the power line regularly; if dust accumulates around the
power pins, there is a risk of fire.
Keep the equipment clean so that the ventilation holds are not
Cleaning obstructed. If the ventilation is obstructed, the system may
overheat and catch fire.
Periodically clean your equipment's case using a damp cloth.
Do not use abrasives, cleaning solvents or strong detergents, as
they may damage the finish or affect the reliability of the
structural components.

If this equipment is used in a manner not specified by this

manual, the protection provided by the equipment may be
impaired.
WEEE Compliance

This product is required to comply with the European Union’s Waste

Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is
marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or

disposal companies in each European Union (EU) Member State, and
these companies should dispose of or recycle this product. See
www.thermo.com/WEEERoHS for further information on Thermo Fisher
Scientific’s compliance with these Directives and the recyclers in your
country.

Conformité DEEE

Ce produit est conforme à la directive européenne (2002/96/EC)

nommée Déchets d'Equipements Electriques et Electroniques (DEEE).
Il est marqué par le symbole suivant:

Thermo Fisher Scientific s'est associé à une ou plusieurs sociétés de

recyclage dans chaque état membre de l’Union Européenne. Ce produit
devra être collecté ou recyclé par celles-ci. Toutes les informations sur la
conformité des produits Thermo Fisher Scientific à ces directives, les
sociétés de recyclage de votre pays et les informations sur les produits
 Thermo Fisher Scientific qui peuvent aider à la détection des substances
sujettes à la directive RoHS sont disponibles sur
www.thermo.com/WEEERoHS.

WEEE Konformität

Dieses Produkt muss die EU Waste Electrical & Electronic Equipment

(WEEE) Richtlinie 2002/96/EC erfüllen. Das Produkt ist durch folgendes
Symbol gekennzeichnet:

Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-

/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen, damit dieses
Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann.
Mehr Information über die Einhaltung dieser Anweisungen durch Thermo
Fisher Scientific, über die Verwerter, und weitere Hinweise, die nützlich
sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung
fallen, finden sie unter www.thermo.com/WEEERoHS.

Conformità RAEE

Si richiede che questo prodotto soddisfi la direttiva dell'Unione Europea

sui rifiuti di apparecchiature elettriche ed elettroniche (RAEE)
2002/96/CE. È contrassegnato col seguente simbolo:

Thermo Fisher Scientific ha concluso accordi con una o più società di

riciclo/smaltimento in ciascuno Stato membro dell'UE, e questo prodotto
 va smaltito o riciclato attraverso tali società. Ulteriori informazioni sulla
conformità di Thermo Fisher Scientific a queste Direttive, sulle società
addette al riciclo nel proprio Paese e informazioni sui prodotti Thermo
Fisher Scientific che potrebbero essere utili nel rilevamento di sostanze
soggette alla Direttiva RoHS sono disponibili all'indirizzo
www.thermo.com/WEEERoHSS.

Conformidad WEEE

Este producto debe cumplir la Directiva 2002/96/CE de la Unión

Europea sobre Desechos eléctricos y de equipos electrónicos (WEEE).
Está marcado con el símbolo siguiente:

Thermo Fisher Scientific ha contactado con una o varias empresas de

reciclaje o eliminación de residuos en cada país miembro de la Unión
Europea (EU) y dichas empresas deben eliminar o reciclar este
producto. Consulte www.thermo.com/WEEERoHS para obtener más
información sobre el cumplimiento de estas directivas por parte de
Thermo Fisher Scientific y sobre las empresas de reciclaje de su país.
FCC verification

This equipment has been tested and found to comply with the
limits for a Class B digital device, pursuant to part 15 of the FCC
Rules. These limits are designed to provide reasonable protection
against harmful interference in a residential installation This
equipment generates uses and can radiate radio frequency energy
and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications,
However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful
interference to radio or television reception, which can be
determined by turning the equipment off and on, the user is
encouraged to try to correct the interference by one or more of the
following measures:

- Reorient or relocate the receiving antenna.

- Increase the separation between the equipment and receiver.
- Connect the equipment into an outlet on a circuit different from
that to which the receiver is connected.
- Consult the dealer or an experienced radio/TV technician for
help.
Equipment Configuration

Standard System configuration

Item Name Qty. Remark

Main
Lumina Fluorescence Spectrometer 1
Instrument
Accessories Power Cord 1 2.0 m

Interface Cable (RS-232) 1 2.0 m

Luminous Software Manual 1

Lumina Installation Guide 1

Luminous Software CD 1

Spare Fuse (AC250 V, T5 AL) 2

Spare Fuse (AC250 V,T 2 AL) 1

 Contents

I. Pre-Installation .........................................................................................................14

I-1. Introduction ....................................................................................................................................... 14

I-2. Preparation for Installation ..............................................................................................................14

I-2-1. Pre-Installation Checklist.............................................................................................................. 14
I-2-2. Environmental Conditions............................................................................................................ 14
I-2-3. Electrical Specifications ............................................................................................................... 16
I-2-4. Equipment on-site ......................................................................................................................... 17

II. Xenon Lamp Replacement .....................................................................................23

II-1. Xenon Lamp Replacement ..............................................................................................................23

II-1-1. Xenon Lamp Replacement Procedure........................................................................................ 25

III. Fuse Replacement..................................................................................................30

III-1. Fuse Replacement...........................................................................................................................30

III-1-1. Replacement of a fuse in the fuse holder ................................................................................. 31
II-1-2. Replacement of a fuse in the noise filter ................................................................................... 32

IV. Power Supply Replacement..................................................................................34

IV-1. Power Supply Replacement...........................................................................................................34

IV-1-1. Replacement of Xenon lamp supply ......................................................................................... 35
IV-1-2. Replacement of SMPS (Switching power supply module) ..................................................... 36

V. Electrical Circuit Board Replacement...................................................................38

V-1. Information on the electrical circuit boards..................................................................................38

V-2. Replacement of Main Controller Board .........................................................................................43

V-2-1. Main Controller Board Replacement Procedure....................................................................... 44

V-3. Replacement of PD (Photo-Diode) Sensor Board ........................................................................ 46

V-3-1. PD (Photo-Diode) Sensor Board Replacement Procedure ...................................................... 47

V-4. Replacement of Xenon Lamp On/Off Check Sensor Board......................................................... 48

V-4-1. Xenon Lamp On/Off Check Sensor Board Replacement Procedure ...................................... 49

V-5. Replacement of LED Display Board...............................................................................................52

V-5-1. LED Display Board Replacement Procedure ............................................................................ 53

V-6. Replacement of Step Motor Board.................................................................................................54

V-6-1. Step Motor Board Replacement Procedure............................................................................... 55

V-7. Replacement of A/D Board .............................................................................................................56

V-7-1. A/D Board Replacement Procedure ........................................................................................... 57

V-8. Replacement of PMT Sensor Board...............................................................................................58

V-8-1. PMT Sensor Board Replacement Procedure............................................................................. 59

V-9. Replacement of Accessories Board ..............................................................................................60

V-9-1. Accessories Board Replacement Procedure ............................................................................ 61

VI. PMT Replacement ..................................................................................................63

VI-1. PMT (Photo Multiplier Tubes) Replacement.................................................................................63

VI-1-1. PMT Replacement Procedure .................................................................................................... 65

VII. Monochromator Replacement .............................................................................68

VII-1. Monochromator Replacement ......................................................................................................68

VII-1-1. Excitation Monochromator Replacement Procedure ............................................................. 70
VII-1-2. Emission Monochromator Replacement Procedure .............................................................. 83

VIII. Shutter Module Replacement .............................................................................92

VIII-1. Shutter Module Replacement ......................................................................................................92

VIII-1-1. Excitation side shutter Module Replacement Procedure ..................................................... 93
VIII-1-2. Emission side shutter Module Replacement Procedure....................................................... 96

IX. Power Switch Module Replacement.....................................................................99

IX-1. Power Switch Module Replacement .............................................................................................99

IX-1-1. Power Switch Module Replacement Procedure..................................................................... 100

X. Wavelength Calibration ........................................................................................103

IX-1. Wavelength Calibration ................................................................................................................103

X-1-1. Emission Monochromator calibration ..................................................................................... 106
X-1-2. Excitation Monochromator calibration .................................................................................... 118

XI. Validation..............................................................................................................123

XI-1. Sensitivity Test – Water Raman ..................................................................................................124

XI-1-1. Sensitivity Test Method ............................................................................................................ 124
XI-1-2. Instrument Condition................................................................................................................ 130

XI-2. Emission wavelength Accuracy Test(1)_ Mercury(Hg) Line ....................................................132

XI-2-1. Emission wavelength Accuracy Test(1) Method ................................................................... 132
XI-2-2. Instrument Condition................................................................................................................ 134

XI-3. Emission wavelength Accuracy Test(2)_ Argon(Ar) Line.........................................................135

XI-3-1. Emission wavelength Accuracy Test(2) Method ................................................................... 135
XI-3-2. Instrument Condition................................................................................................................ 137

XI-4. Excitation wavelength Accuracy Test ........................................................................................138

XI-4-1. Excitation wavelength Accuracy Test Method....................................................................... 138
XI-4-2. Instrument Condition................................................................................................................ 140

XI-5. Excitation wavelength Accuracy Test and Repeatability Test-0nm ........................................141

XI-5-1. Excitation wavelength Accuracy Test and Repeatability Test Method ............................... 141
XI-5-2. Instrument Condition................................................................................................................ 143

XI-6. Emission wavelength Accuracy Test and Repeatability Test-0nm .........................................144

XI-6-1. Emission wavelength Accuracy and Repeatability Test Method......................................... 144
XI-6-2. Instrument Condition................................................................................................................ 146

XI-7. Reporting Validation Result.........................................................................................................147

XI-7-1. Print Report Cover .................................................................................................................... 147
XI-7-2. Print Validation Report ............................................................................................................. 150
XI-7-3. Print Detailed Validation Results............................................................................................. 151

XII. Correction Spectra..............................................................................................152

XII-1. Correction Spectra.......................................................................................................................152

XII-1-1. Generating emission correction factors................................................................................ 153
XII-1-2. Generating excitation correction factors............................................................................... 159

XIII. Diagnostic Software ..........................................................................................164

XIII-1. Diagnostic Software ...................................................................................................................164

XIII-1-1. How to execute Diagnostics Software. ................................................................................. 165

XIII-2. Diagnostics Error Chart .............................................................................................................173

XIV. Firmware Upgrade .............................................................................................181

XIV-1. Updating the firmware of main board.......................................................................................181

XIV-1-1. Installation of the Firmware Update Utility for main B/D update ....................................... 181

XIV-2. Updating the firmware of main B/D using update utility software ........................................184

XIV-3. Replacement of Accessory B/D ................................................................................................190

XV. Troubleshooting .................................................................................................193

 I. Pre-Installation

I-1. Introduction
Lumina fluorescence spectrometer is designed to provide a complete analysis system
for fluorescence, phosphorescence and chemiluminescence measurements.
All instruments will be tested and proven to the performance before the shipment

I-2. Preparation for Installation

I-2-1. Pre-Installation Checklist

Preparation Done

The installation place should be prepared / checked. □

The electric power is available / stable. □

The system and accessories should be unpacked on site. □

Proper computer system should be prepared. □

Windows operating system should be prepared. □

Quartz cell for fluorescence is available. □

Operation training is required. □

I-2-2. Environmental Conditions

You are responsible for providing an acceptable operating environment. Attention paid
to the operating environment well ensures the continued high performance of your
Lumina fluorescence instrument. The instrument warranty will be made void if the
equipment is operated under sub-standard conditions.

Thermo Scientific Lumina Service Manual 14

 Humidity

The relative humidity of the operating environment should be lower than Maximum
75% with no condensation. Operating the Lumina fluorescence in very low humidity
may result in the accumulation and discharge of static electricity that shortens the
life of electronic components. Operating at high humidity will produce condensation
and result in short circuits. Thermo Scientific recommends that your laboratory be
equipped with a temperature / humidity monitor. This will ensure that your laboratory
is always in conformance with the temperature and humidity specifications.

Temperature

Air conditioning is recommended. The room should be temperature controlled if

your analyses are particularly sensitive. For optimum analytical performance it is
recommended that the ambient temperature of the laboratory be between
15-35℃ and be held constant to within +/-2% throughout the entire working day.

As laboratory temperature increases, system reliability decreases and all

electronic components generate heat while operating. This heat must be
dissipated to the surrounding air so that the components are operating correctly.

Vibration

Ensure that lab benches are free from vibration. If possible, any equipment
generating vibration during the operation should be installed on the floor rather than
alongside the system on the workbench.

Work bench

The workbench must be stable and durable enough to support the total weight of
equipment. The worktops should be large enough to permit a free circulation of air
around each unit in the system. Remember to provide space for the computer,
monitor and printer. To avoid damage through spillage of the samples being
analyzed, the worktops should be covered with a material which is corrosion
resistant and impervious to liquids.

15 Lumina Service Manual Thermo Scientific

 ** Lumina dimensions are 620 mm (W) x 710 mm (L) x 300 mm (H)

I-2-3. Electrical Specifications

In view of the electronic components in the Lumina system, it

should be positioned well away from any instruments emitting an
electromagnetic field and any environment that may cause
vibration.

Power supply

All power supplies must be single phase AC voltage, three wire system (active,
neutral, ground) and should be terminated at an appropriate power outlet and/or
regulations imposed by local authorities responsible for the supply of electrical energy
to the workplace.

A grounded AC power supply must be used. The Lumina accepts a dual voltage of

either 110 or 220V at 50 ㎐ or 60 ㎐ and requires 300 W power including computer

and printer.

Good electrical grounding is essential to avoid potentially

hazardous shock hazards. 3-wire outlet with ground connection
must be provided for the instrument. Make certain that power
outlets are grounded at the grounding pin.

Avoid using power supplies from a source that may be subject to electrical or RF
interference from other services (large electric motors, elevators, and welders for
example).

Two fuses are used in the Lumina fluorescence spectrometer;

Fuse A: AC 250V, T2AL
Fuse B: AC 250V, T5AL

Thermo Scientific Lumina Service Manual 16

 I-2-4. Equipment on-site

Insurance

As the carrier’s liability ceases when equipment is delivered, Thermo Scientific

recommends that the instrument owner arrange a separate insurance to cover
transportation from the delivery point to the installation site. The delivery point will vary
according to the carrier, the shipping method and in some cases the terms of sale. Some
carriers will deliver only to their own distribution centers, who then will deliver to the
actual installation site.

Inspection for transit damage

Transit damage can be obvious or concealed and in either case will be admitted by the
carrier only if it is reported within the terms of the carrier’s agreement. For any claims
against damage in transit, the following general rules apply;

- Before accepting delivery, you must inspect the packages for signs of obvious
damage. The nature of any obvious damage must be noted on the carrier’s waybill,
which then must be countersigned by a representative of the carrier

- Within the time limit stated in the terms and conditions of carriage, a further inspection
must be made for concealed damage. If any damage is found at this stage, the carrier
must be modified in writing. You must retain all packing material for subsequent
inspection by a representative of the carrier.

- A copy of any damage report must be forwarded to Thermo Scientific or Sales

representative

- ‘SHOCK WATCH vibration indicator label that helps you to aware of the damage level
are attached on Packing Boxes. If the indicator color is changed as red color, it means
the packing box could be impacted/damaged by the physical shock externally.
If it is happened, contact Thermo Scientific or sales representative immediately to hear
the next procedures.

17 Lumina Service Manual Thermo Scientific

 (* Take a picture to provide the insurance company for the evidence if it is
Available)

Environmental Requirements

Indoor use only

Altitude up to 2000m

Pollution degree 2

Dimensions 620 (W) x 710 (L) x 300 (H)

Electrical supply AC 100-240V, 50/60Hz, 240W

Operating Temperature 15 - 35

Operating humidity Maximum 75% relative, non-condensing

Main Supply Voltage Fluctuation ± 10%

Ventilation Air Cooling System (DC FAN)

Main Part Specification for Lumina

1. Concave Mirror
- Focal length: 25mm
- Coating: Aluminum
- 25.4mm Diameter

Thermo Scientific Lumina Service Manual 18

 2. Light Source
- Xenon discharge lamp for DC operation
- Short arc, High pressure
- Rated lamp current : 7.5A
- Rated lamp power: 150W
3. Condenser Lens
- Focal length: 50mm, Focal length: 150mm
- Material: Fused Silica
- 25.4mm Diameter

4. Plane Mirror
- Substrate Material : UV-grade fused silica
- Coating: Deep UV aluminum
- 50mm Diameter

5. Photodiode Detector
- Spectral Range: 190nm to 1100nm
- Packing: Metal
- 13.9mm Diameter

6. Concave Mirror
- Focal length: 150mm
- Substrate Material: UV-grade fused silica
- Coating: Deep UV aluminum
- 50mm Diameter

7. Slit (Excitation slit is same as Emission slit)

- 0.5nm, 1nm, 2.5nm, 5nm, 10nm, 20nm

8. Filter (Excitation slit is same as Emission slit)

- Long Pass Filter 12.5mm Diameter, 530nm
- Long Pass Filter 12.5mm Diameter, 435nm
- Long Pass Filter 12.5mm Diameter, 320nm

19 Lumina Service Manual Thermo Scientific

 9. Grating (Excitation side)
- Plane Blazed Holographic Grating
- Blaze Wavelength: 250nm
- Groove density: 1200 grooves/mm
- Outside dimension: 34.0mm(W) x 34.0mm(H)

10. Xenon Power Supply

- Power supply for Xenon short Arc lamps
- Input voltage range from 90V AC to 264V AC
- Power factor corrected line input, built-in EMI filter

11. Concave Mirror

- Focal length: 200mm
- Substrate Material: UV-grade fused silica
- Coating: Deep UV aluminum
- 50mm Diameter

12. Cooling Fan

- DC 24V, 0.21A, 3300RPM

13. Power Switch (Noise filter included)

- Rating IEC : 1-10A @ Ta 40’C/250VAC: 50Hz
- Rating UL/CSA: 1-10A @ Ta 40’C/125VAC: 60Hz

14. Grating (Emission side)

- Plane Blazed Holographic Grating
- Blaze Wavelength: 400nm
- Groove density: 1200 grooves/mm
- Outside dimension: 34.0mm(W) x 34.0mm(H)

15. Main Power Supply (total 3ea)

- Power_1: Output voltage: 24V,
Max output current: 4.5A,
Max output power: 108W

Thermo Scientific Lumina Service Manual 20

 - Power_2: Output voltage: 5V, 12V,
Total output power: 75W

- Power_3: Output voltage: 24V,

Output current: 1.5A

16. Photomultiplier (R928)

- Spectral Response: 185nm to 900nm
- High Cathode Sensitivity, High Anode Sensitivity
- Low Drift and Hysteresis

17. Condenser Lens

- Focal length: 50mm, Focal length: 100mm
- Material: Fused Silica
- 25.4mm Diameter

18. Quartz Window

- Material: Quartz
- 25.4mm Diameter

21 Lumina Service Manual Thermo Scientific

Thermo Scientific Lumina Service Manual 22
 II. Xenon Lamp Replacement

II-1. Xenon Lamp Replacement

The Xenon lamp for replacement is packed in the manufacturer’s box and must be
installed in the lamp housing correctly. Read all the precautions before attempting to
replace the Xenon lamp.

Caution

A. Xenon arc lamp has an explosion hazard. Make sure the system’s power is off
and all AC power is disconnected from the system. Read and follow the cautions
presented below.

B. Xenon arc lamp has an explosion hazard. Wear goggles and protective clothing
when opening the lamp housing and when handling the lamp.

C. The lamp will remain extremely hot for approximately one-half hour after it has
been turned off. Do not touch the lamp or the metal unit until the lamp has cooled.

D. Never look directly at the xenon arc or its reflection. Intense radiation can
permanently damage eyes.

E. Do not touch the focusing lens, back scatter mirror, or the surface of the lamp.
Fingerprints will be burned onto these surfaces when the lamp is ignited.

23 Lumina Service Manual Thermo Scientific

 Xenon Lamp Replacement Flow Chart

1) Start
2) Turn off the power of the instrument.
3) Remove white screw caps (total 10) located around on the upper case.
4) Remove Phillips Head screws using a Phillips screwdriver.
5) Remove Phillips head screws (4 ea) located on the Sample Cell Assay part using a
Phillips screwdriver.
6) Separate the Sample Cell Assay part and lift the upper case.
7) Get rid of socket screws (4 ea) using a hexa socket screwdriver.
8) Separate the lamp connector from the lamp power module and push the hap and pull
out the connector.
9) Remove the lamp mounting module from the lamp housing.
10) Remove the screws (2 ea) located on the edges of the lamp.
11) Unfasten the socket screws (2ea) located on the (+) side of the Teflon bracket.
12) Unpack a new Xenon lamp.
13) Install the lamp in the correct polarity.
14) Fasten the socket screw (2ea) located in (+) direction Teflon bracket. Put the changed
lamp module back in the lamp housing.
15) Finally, connect the lamp cable to the power supply cable.
16) End

Thermo Scientific Lumina Service Manual 24

 II-1-1. Xenon Lamp Replacement Procedure

1. Check the main power off (If the system and lamp are on, it requires enough cooling
time of lamp over 10 minutes after turning off the control board and lamp. Then, turn off
the sub main power switch at the bottom right side of the front panel and the main
power on the rear panel)

2. Remove white screw caps (total 10 ea) located around on the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.

25 Lumina Service Manual Thermo Scientific

 4. Remove Phillips head screws (4ea) located on the compartment using a Phillips
screwdriver.

5. Separate the sample compartment and lift the upper case.

6. Get rid of socket screws (4ea) using a hex socket screwdriver.

Thermo Scientific Lumina Service Manual 26

 7. Separate the lamp connector from the lamp power module and push the hap and pull
out the connector.

8. Remove the lamp mounting module from the lamp housing.

9. Remove the screws (2ea) located on the edges of the lamp.

27 Lumina Service Manual Thermo Scientific

 10. Unfasten the socket screws (2ea) located on the (+) side of the Teflon bracket.

11. Unpack a new Xenon lamp.

12. Install the lamp in the correct polarity.

Caution:
Install the lamp in the correct polarity. Otherwise it could cause burst, overheating of the
lamp house or short lamp life. (Upper side is plus polarity)

13. Do not use any tools to tighten nuts when fixing the lamp, use hands only. Otherwise it
could cause breakage.

Thermo Scientific Lumina Service Manual 28

 14. Fix the lamp and its lead wire firmly to the terminal.

15. Fasten the socket screw (2ea) located in (+) direction Teflon bracket. Put the changed
lamp module back in the lamp housing.

16. Finally, connect the lamp cable to the power supply cable.

29 Lumina Service Manual Thermo Scientific

 III. Fuse Replacement

III-1. Fuse Replacement

Flow Chart of the Fuse Replacement in the fuse holder

1) Start
2) Turn off the power of the instrument.
3) Loosen the fuse holder on the rear side of the instrument.
4) Pull out the fuse (AC 250V, T2AL) and exchange it with a new one.
5) Put the fuse holder back and tighten.
6) End

Flow Chart of the Fuse Replacement in the noise filter

1) Start
2) Turn off the power of the instrument.
3) Pull out the fuse box on the rear side of the instrument.
4) Replace the fuse (AC 250V, T5AL) with a new one.
5) Put the fuse box back as it was.
6) End

Thermo Scientific Lumina Service Manual 30

 III-1-1. Replacement of a fuse in the fuse holder

1. Check the main power off (If the system and lamp are on, it requires enough cooling
time of lamp over 10 minutes after turning off the control board and lamp. Then, turn off
the sub main power switch at the bottom right side of the front panel and the main
power on the rear panel)

2. Loosen the fuse holder by turning it clockwise on the rear side of the instrument, using
(-) driver

3. Pull out the fuse (AC 250V, T2AL) and exchange it with a new one.

31 Lumina Service Manual Thermo Scientific

 4. Put the fuse holder back and tighten.

Caution: Should use the fuse of rated capacity (AC 250V, T2AL).

II-1-2. Replacement of a fuse in the noise filter

1. Check the main power off (If the system and lamp are on, it requires enough cooling
time of lamp over 10 minutes after turning off the control board and lamp. Then, turn off
the sub main power switch at the bottom right side of the front panel and the main
power on the rear panel)

2. Pull out the fuse box on the rear side of the instrument as below.

Thermo Scientific Lumina Service Manual 32

 3. Replace the fuse (AC 250V, T5AL) with a new one.

Caution: Should use the fuse of rated capacity (AC 250V, T5AL).

4. Put the fuse box back as it was.

33 Lumina Service Manual Thermo Scientific

 IV. Power Supply Replacement

IV-1. Power Supply Replacement

Flow Chart of the Xenon lam power Replacement

1) Start
2) Turn off the power of the instrument.
3) Check the Xenon lamp power supply’s location before starting replacement work.
4) Disconnect xenon lamp power cable, AC power connector and On/Off signal
connector.
5) Unfasten the socket screws (4ea) located on the base plate of the Xenon lamp
power supply.
6) Replace the Xenon lamp power supply with a new one and re-assemble in reverse
order.
7) End

Flow Chart of the SMPS (Switching power supply module) Replacement

1) Start
2) Turn off the power of the instrument.
3) Separate cables connected from the SMPS.Disconnect xenon lamp power cable,
AC power connector and On/Off signal connector.
4) Unfasten the socket screws (4ea) located on the base plate.Replace the Xenon
lamp power supply with a new one and re-assemble in reverse order.
5) After replace the SMPS, reconnect the cables to the new SMPS.
6) End

Thermo Scientific Lumina Service Manual 34

 IV-1-1. Replacement of Xenon lamp supply

1. Check the main power off (If the system and lamp are on, it requires enough cooling
time of lamp over 10 minutes after turning off the control board and lamp. Then, turn off
the sub main power switch at the bottom right side of the front panel and the main
power on the rear panel)

2. Check the Xenon lamp power supply’s location before starting replacement work.

3. Disconnect xenon lamp power cable, AC power connector and On/Off signal connector.

4. Unfasten the socket screws (4ea) located on the base plate of the Xenon lamp power
supply.

35 Lumina Service Manual Thermo Scientific

 5. Replace the Xenon lamp poser supply with a new one and re-assemble in reverse
order.

IV-1-2. Replacement of SMPS (Switching power supply module)

1. Turn off the power of the instrument.

Tip: separate the rear panel for convenience replacement.

2. Separate cables connected from the SMPS

Thermo Scientific Lumina Service Manual 36

 3. Unfasten the socket screws (4ea) located on the base plate.

4. After replace the SMPS, reconnect cables to the new SMPS.

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 V. Electrical Circuit Board Replacement
V-1. Information on the electrical circuit boards

Accessories Board

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 Information on the main controller board and connector

1 Excitation Grating(EXG) Motor

2 Emission Grating(EMG) Motor
3 Excitation Filter(EXF) Motor
4 Excitation Slit(EXS) Motor
5 Emission Slit (EMS) Motor
6 Emission Filter(EXF) Motor
7 AD Board
8 Solenoid Sutter
9 Xenon Check Board
10 RS-232 Cable
11 LED Board
12 SMPS Power Cable

39 Lumina Service Manual Thermo Scientific

 Step motor board for the Slit and the Filter module

Step motor board for the Grating module

Electrical Circuit Board for the LED

Thermo Scientific Lumina Service Manual 40

 Electrical Circuit Board for the PD Sensor

Electrical Circuit Board for the AD Board

Electrical Circuit Board for the Xenon lamp on/off check Board

41 Lumina Service Manual Thermo Scientific

 Electrical Circuit Board for the PMT Sensor Board

Accessories Board

Thermo Scientific Lumina Service Manual 42

 V-2. Replacement of Main Controller Board

Flow Chart of replacement of Main Controller Board

1) Start
2) Turn off the power of the instrument.
3) Separate all connectors from the main controller Board.
4) Remove the Phillips screws (8ea) located on the main controller board bracket (the
red-colored marking point) and separate the main controller board from the
bracket.
5) Remove the Phillips screws (2ea) located on the step motor board from the main
controller board bracket (the red-colored marking point) and separate the step
motor board from the main controller board.
6) Remove the Phillips screws (2ea) located on the A/D board from the main
controller board (red-colored marking point) and separate the A/D board form the
main controller board.
7) Replace the main controller board and reassemble all the separated items (Step
motor board, A/D board, Cable connectors) in reverse order.
8) End

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 V-2-1. Main Controller Board Replacement Procedure

1. Turn off the power of the instrument.

Tip: Remove the Board Protect cover for convenience replacement.

2. Separate all connectors from the Main Controller Board.

3. Remove the Phillips screws (8 ea) located on the main controller board bracket (the
red-colored marking point) and separate the main controller board from the bracket.

Thermo Scientific Lumina Service Manual 44

 4. Remove the Phillips screws (2 ea) located on the step motor board from the main
controller board bracket (red-colored marking point) and separate the step motor board
from the main controller board.

5. Remove the Phillips screws (2 ea) located on the A/D board from the main controller
board (red-colored marking point) and separate the A/D board from the main controller
board.

6. Replace the main controller board and reassemble all separated items (Step motor
board, A/D board, Cable connectors) in reverse order.

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 V-3. Replacement of PD (Photo-Diode) Sensor Board

Flow Chart of replacement of the PD (Photo-Diode) Sensor Board

1) Start
2) Turn off the power of the instrument and check the location of the PD (Photo-
Diode) sensor board.
3) Separate all connectors, release the snap keys and lift the optical housing
connected with the PD sensor board.
4) Remove the Phillips screws (4ea) _ (red-colored marking point) located on the PD
sensor board and separate the board from optical housing.
5) Replace the PD Sensor Board with a new one and reassemble all the connectors
in reverse order.
6) End

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 V-3-1. PD (Photo-Diode) Sensor Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the PD (Photo-Diode)
sensor board.

2. Separate all connectors, release the snap keys and lift the optical housing connected
with the PD sensor board.

3. Remove the Phillips screws (4 ea) (red-colored marking points) located on the PD
sensor board and separate the board from the optical housing.

47 Lumina Service Manual Thermo Scientific

 4. Replace the PD Sensor Board with a new one and reassemble all the connectors in
reverse order.

V-4. Replacement of Xenon Lamp On/Off Check

Sensor Board

Flow Chart of replacement of Xenon Lamp On/Off Check Sensor Board

1) Start
2) Turn off the power of the instrument and check the location of the Xenon
Lamp On/Off Check Board and lift the optical housing after releasing the snap keys.
3) Remove the socket screws (6ea) located on the optical base plate (red color
marking point) and lift the optical base plate.
4) Remove the Phillips screws (2ea) located on the optical housing which is adapted
to the On/Off Check Sensor Board, and lift the optical housing.
5) Remove the Phillips screws (4ea) located on the Xenon On/Off Check Sensor
Board (red color marking point) and Separate the Xenon On/Off Check Sensor
Board from the optical housing.
6) Reinstall a new Xenon On/Off Check Sensor Board in the optical housing and
connect all the connectors in reverse order.
7) End

Thermo Scientific Lumina Service Manual 48

 V-4-1. Xenon Lamp On/Off Check Sensor Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the Xenon Lamp On/Off
Check Board and lift the optical housing after releasing the snap keys.

2. Remove the socket screws (6 ea) located on the optical base plate (red color marking
point) and lift the optical base plate.

49 Lumina Service Manual Thermo Scientific

 3. Remove the socket screws (2 ea) located on the optical housing which is adapted to
Xenon On/Off Check Sensor Board, and lift the optical housing.

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 4. Remove the Phillips screws (4 ea) located on the Xenon On/Off Check Sensor Board
(red color marking point) and Separate the Xenon On/Off Check Sensor Board from the
optical housing.

5. Reinstall a new Xenon On/Off Check Sensor Board in the optical housing and connect
all the connectors in reverse order.

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 V-5. Replacement of LED Display Board

Flow Chart of replacement of LED Display Board

1) Start
2) Turn off the power of the instrument and check the location of the LED Display
Board.
3) Separate the cable connector and unfasten the nut screws (4ea), the bolt screws
(4ea) on the LED display Board.
4) Reinstall a new LED Display Board in the Front panel and connect all the
connectors in reverse order.
5) End

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 V-5-1. LED Display Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the LED Display Board.

2. Separate the cable connector and unfasten the nut screws (4ea) located on the LED
Display Board.

3. Reinstall a new LED Display Board in the Front panel and connect all connectors in
reverse order.

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 V-6. Replacement of Step Motor Board

Flow Chart of replacement of Step Motor Board

1) Start
2) Turn off the power of the instrument and check the location of the step motor board
for the slit and the filter module, and separate all connectors from the main
controller board.
3) Remove the Phillips screws located on the main controller board (red color
marking point) and separate the step motor board for the slit/filter module and the
step motor board for the grating module from the Main controller board.
4) Reinstall the new step motor boards and connect all the connectors in reverse
order.
5) End

Thermo Scientific Lumina Service Manual 54

 V-6-1. Step Motor Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the step motor board for
the slit and the filter module, and separate all connectors from the main controller
board.

2. Remove the Phillips screws located on the main controller board (red color marking
point) and separate the step motor board for the slit / filter module and the step motor
board for the grating module from the Main controller board.

3. Reinstall the new step motor board and connect all connectors in reverse order.

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 V-7. Replacement of A/D Board

Flow Chart of replacement of A/D Board

1) Start
2) Turn off the power of the instrument and check the location of the A/D board and
separate all the connectors from the main controller board.
3) Remove the Phillips screws located on the main controller board (red color
marking point) and separate the A/D Board from the main controller board.
4) Reinstall the new A/D boards with the main controller board and connect all the
connectors in reverse order.
5) End

Thermo Scientific Lumina Service Manual 56

 V-7-1. A/D Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the A/D board and
separate all connectors from the main controller board.

2. Remove the Phillips screws located on the main controller board (red color marking
point) and separate the A/D Board from the main controller board.

3. Reinstall a new A/D Board with the main controller board and connect all the
connectors in reverse order.

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 V-8. Replacement of PMT Sensor Board

Flow Chart of replacement of PMT Sensor Board

1) Start
2) Turn off the power of the instrument and check the location of the PMT sensor
board and separate all the connectors from the main controller board.
3) Remove the Phillips screws located on the main controller board for the PMT
Sensor board (red color marking point) and separate the PMT Sensor.
4) Reinstall a new PMT Sensor board in the main controller board and connect all the
connectors in reverse order.
5) End

Thermo Scientific Lumina Service Manual 58

 V-8-1. PMT Sensor Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the PMT sensor board
and separate all the connectors from the main controller board.

2. Remove the Phillips screws located on the Main controller for the PMT Sensor board
(red color marking point) and separate the PMT Sensor.

3. Reinstall a new PMT Sensor board in the main controller board and connect all the
connectors in reverse order.

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 V-9. Replacement of Accessories Board

Flow Chart of replacement of Accessories Board

1) Start
2) Turn off the power of the instrument and check the location of the Accessories
board and separate all the connectors from the main controller board.
3) Separate the sample compartment and check the position of accessories
connector panel.
4) Separate all the connectors from the main controller board and Accessories
connector panel.
5) Remove the screws located on the accessories board
6) Reinstall a new accessories board and connect all the connectors in reverse order
7) End

Thermo Scientific Lumina Service Manual 60

 V-9-1. Accessories Board Replacement Procedure

1. Turn off the power of the instrument and check the location of the Accessories board
and separate all the connectors from the main controller board.

2. Separate the sample compartment and check the position of accessories connector
panel.

3. Separate all the connectors from the main controller board and Accessories connector
panel.

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 4. Remove the screws located on the accessories board.

5. Reinstall a new accessories board and connect all the connectors in reverse order.

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 VI. PMT Replacement
VI-1. PMT (Photo Multiplier Tubes) Replacement
A high voltage is applied to PMT during operation. Always provide adequate safety
measures to prevent the operator or service personnel from electrical shock and the
equipment from being damaged.

Caution
A. Handle tubes with extreme care.
PMT has evacuated glass envelopes, allowing the glass to be scratched or subjected to
shock can cause cracks. Take extreme care during handling, particularly for tubes with
graded sealing on synthetic silica bulbs.

B. Keep faceplate and base clean.

Do not touch the faceplate and base with bare hands. Dirt and grime on the faceplate
causes loss of transmittance and dirt or grime on the base may cause ohmic leakage. If
the faceplate becomes soiled, wipe it clean using alcohol.

C. Do not expose to strong light.

The PMT may be damaged if exposed to direct sunlight or intense illumination. Never
allow strong light to strike the photocathode.

D. Carefully handle tubes with a glass base.

PMT with a glass base (also called button stem) are less rugged than tubes with a
plastic base, so sufficient care must be taken when handling this type of tube. When
fabricating a voltage-divider circuit by soldering resistors and capacitors to socket lugs,
solder them while the tube is fully inserted into the socket.

E. Helium permeation through silica bulb.

Helium will permeate through silica bulbs and increase noise, leading to damage that
makes PMT unusable. Avoid operation or storing them in and atmosphere where helium
is present

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 PMT Replacement Flow Chart

1) Start
2) Turn off the power of the instrument and check the location of the PMT Sensor
Board.
3) Separate all the connectors (red color marking point) from the main controller board.
4) Remove the Phillips screws (4ea) located on the PMT bracket (red color marking point).
5) Draw out the PMT carefully.
6) Reinstall a new PMT with the PMT sensor bracket and connect all the connectors in
reverse order.
7) End

Thermo Scientific Lumina Service Manual 64

 VI-1-1. PMT Replacement Procedure

1. Turn off the power of the instrument and check the location of the PMT Sensor board.

2. Separate all the connectors (red color marking point) from the main controller board.

65 Lumina Service Manual Thermo Scientific

 3. Remove the Phillips screws (4ea) located on the PMT bracket (red color marking point).

Caution:
We recommend this method is performed in dark room for protecting PMT.

4. Draw out the PMT carefully.

Thermo Scientific Lumina Service Manual 66

 5. Reinstall a new PMT with the PMT sensor bracket and connect all the connectors in
reverse order.

Caution

Check out the direction of the PMT socket assembly pin array before the reinstallation of
the PMT sensor in the PMT socket assembly.

67 Lumina Service Manual Thermo Scientific

 VII. Monochromator Replacement

VII-1. Monochromator Replacement

Monochromator is dispersing polychromatic or white light into the various colors or
wavelengths. This dispersion can be accomplished using diffraction gratings.
The performance specifications of a monochromator include the dispersion, the efficiency,
and the stray light levels. It is important to recognize that transmission efficiency of a
grating and monochromator is dependent on wavelength and on the design of the grating.
Moncochromator consists of a grating, a step motor, reflect mirrors, a worm gear, a worm
wheel, and photo interrupt sensors.

Thermo Scientific Lumina Service Manual 68

 Excitation Monochromator Replacement Flow Chart

1) Start
2) Turn off the power of the instrument.
3) Disassemble each housing part and connected cables. And Disconnect the Fan
connectors (2ea) and disassemble the rear panel to pull the excitation monochromator
out from the base plate.
4) Exchange the defected monochromator with a new monochromator.
And reassemble the cables and the housing part.
5) Monochromator Alignment.
6) Slit calibration, Filter calibration, and monochromator wavelength calibration.
7) End

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 VII-1-1. Excitation Monochromator Replacement Procedure

1. Turn off the power of the instrument.

or

2. Remove white screw (total 10) located around on the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.

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 4. Remove Phillips head screws (4 ea) located on the sample compartment using a
Phillips screwdriver.

5. Separate the Sample compartment and lift the upper case.

71 Lumina Service Manual Thermo Scientific

 6. Disconnect the Fan connectors (2ea), RS-232 connector on main board and
disassemble the rear panel to pull the excitation monochromator out from the base
plate.

7. Disconnect the worm drive step motor cable, the filter step motor cable, and the slit
step motor cable. Remember each pin position and label number for re-connection.

Thermo Scientific Lumina Service Manual 72

 8. Unscrew socket the head screws ( total 4ea)

9. Pull monochromator out as shown in the right-picture below to release its connector
conveniently.

10. After the removal of the defected monochromator is completed, insert a new
monochromator to the base plate and place it in the suitable screw position.

73 Lumina Service Manual Thermo Scientific

 11. Re-connect three cables.

Be careful to connect them to each the right position. If the cables are not connected
in the proper position, the components would not be operated properly.

Check the correct cable positions referring to the below figures.

1 Excitation Grating(EXG) Motor

2 Emission Grating(EMG) Motor
3 Excitation Filter(EXF) Motor
4 Excitation Slit(EXS) Motor
5 Emission Slit (EMS) Motor
6 Emission Filter(EMF) Motor

Thermo Scientific Lumina Service Manual 74

 12. Fasten the socket head screws (total 4ea). Do not fasten screws tightly since
Monochromator need to be moved slightly for beam Alignment.

13. Run the ‘Alignment.exe’ for alignment procedure located in Lumious Software folder

75 Lumina Service Manual Thermo Scientific

 14. Turn on the xenon lamp, and Calibrate Slits and Filters using Alignment software.
After finishing calibration of slits and filters, set the filter as ‘air blank’ and the slit 2.5nm
and move the grating to zeroth order for beam alignment.

Thermo Scientific Lumina Service Manual 76

 15. Remove the side panel of monochromator to observe the beam path of Xenon lamp
which pass through the collimator lens, slit, filter and reach to the first reflect mirror.

[Power supply]
The power supply of the xenon lamp is extremely dangerous, it generates the high
voltages so take a great care to the electric shock.

[Xenon lamp]
Xenon lamps emit ultraviolet radiation which is harmful to the eyes and the skin so
Always wear a light-shielding protector while the lamp is on.

[Grating]
1) Never touch grating surface. Crusts do not affect on optical characteristics in most
cases but fingerprints cause surface deterioration.
2) Do not wipe the sides of grating by solvent and /or clip the edges of grating plane.
When liquids and/or oil ingredients are adhered in the area of the edges of grating

77 Lumina Service Manual Thermo Scientific

 plane, they might intrude into grating plane alongside of grooves and diffuse over
grating plane.
3) Do not rinse out gratings by water and/or solvent. Also, be sure not to expose
gratings to chemicals and/ or that vapor which might exert harmful influences on
resin and/or coating materials of gratings. They cause surface deterioration.
4) Use gratings in low dust environment. Do not breathe upon grating surface when
dust and/or dirt are accreted. Gently blow away by dry and clean air.
5) Use gratings in no-dew-condensation environment. Dew condensation causes
eminent deterioration of optical characteristics.

[Air Fan]
Disconnect the cable of air blow fan for protection of grating during the service.
Oil of DC motor could attack the surface of grating.

16. 1) Place the upper optic module and screw socket head screw. (Refer to below
figure.)
2) Open the shutter.
3) Put the Teflon Block in the center of cell compartment. (Refer to below
figure.)

Thermo Scientific Lumina Service Manual 78

 4) Check whether bean alignment is no problem. If there are problems, move
monochromator position_①adjust and tilt reflectance mirror._② (Refer to below

figure.)

79 Lumina Service Manual Thermo Scientific

Thermo Scientific Lumina Service Manual 80
 17. After Xenon Lamp Beam Alignment is completed, fasten Excitation monochromator
carefully for the beam alignment not to be distorted. Fasten the socket head screws
(total 4ea) crossed by the slanting lines to prevent excitation monochromator alignment
from distorting.

18. Reassemble the upper optical protect housing, the xenon lamp protect housing, the
side panel of monochromator, the upper case and the sample cell assay.

19. Finally, make monochromator wavelength recalibration referring to chapter 10.

wavelength calibration.

81 Lumina Service Manual Thermo Scientific

 Emission Monochromator Replacement Flow Chart

1) Start
2) Turn off the power of the instrument.
3) Disassemble each housing part and connected cables.
4) Exchange the defected monochromator with a new monochromator.
And reassemble the cables and the housing part.
5) Monochromator Alignment.
6) Slit calibration, Filter calibration, and monochromator wavelength calibration.
7) End

Thermo Scientific Lumina Service Manual 82

 VII-1-2. Emission Monochromator Replacement Procedure

1. Turn off the power of the instrument.

or

2. Remove white screw (total 10) located around on the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.

83 Lumina Service Manual Thermo Scientific

 4. Remove Phillips head screws (4 ea) located on the sample compartment using a
Phillips screwdriver.

5. Separate the sample compartment and lift the upper case.

Thermo Scientific Lumina Service Manual 84

 6. Disconnect the worm drive step motor cable, the filter step motor cable, and the slit
step motor cable. Remember each pin position for reconnection.

7. Disconnect the Fan connectors (2ea) and disassemble the rear panel to pull the
emission monochromator out from the base plate.

85 Lumina Service Manual Thermo Scientific

 8. Unscrew the socket head screws (total 4ea) and pull the emission monochromator out
from the base plate carefully.

9. Pull out the PMTbulb from the PMT housing and reassemble it to a new PMT housing
of emission monochromator carefully.

Caution
We recommend this method is performed in dark room for protecting PMT.

[PMT handing]
A. Handle tubes with extreme care.
PMT has evacuated glass envelopes, allowing the glass to be scratched or

Thermo Scientific Lumina Service Manual 86

 subjected to shock can cause cracks. Take extreme care during handling, particularly
for tubes with graded sealing on synthetic silica bulbs.
B. Keep faceplate and base clean.
Do not touch the faceplate and base with bare hands. Dirt and grime on the
faceplate causes loss of transmittance and dirt or grime on the base may cause
ohmic leakage. If the faceplate becomes soiled, wipe it clean using alcohol.

10. Put a new emission monochromator into the base plate. Reconnect all the cables of
the worm drive step motor, the filter step motor and the slit step. All the cables should
be connected in their proper positions.

87 Lumina Service Manual Thermo Scientific

 11. Fasten the socket head screws (total 4ea). Pleas don’t fasten screws tightly since
Monochromator need to be moved slightly for beam Alignment.

12. Run the ‘Alignment.exe’ for alignment procedure located in Luminous Software folder

13. Turn on the xenon lamp, and Calibrate Slits and Filters using Alignment software.
After finishing calibration of slits and filters, set the filter as ‘air blank’ and the slit 2.5nm
and move the Excitation monochromator grating to zeroth order for beam alignment.

Thermo Scientific Lumina Service Manual 88

 [Xenon lamp]
Xenon lamps emit ultraviolet radiation which is harmful to the eyes and the skin so
always wear a light-shielding protector while the lamp is on.

14. Install Teflon block in the cell holder. The Teflon block (PN FS2-ACCE-08) reflects the
xenon lamp beam from the excitation lens holder to the emission lens holder and that
will be helpful to make an emission monochromator alignment. Generally, both slit
widths will be set as 2.5nm.

89 Lumina Service Manual Thermo Scientific

 15. Using the reflect mirror (PN FS2-ACCE-02), you can find the xenon lamp beam position
in the entrance slit of the emission monochromator.

16. Install the emission monochromator in the proper position, monitoring xenon lamp
beam position in the entrance slit of the emission monochromator. After the beam
alignment is completed, fasten the emission monochromator carefully for the beam
alignment not to be distorted. Fasten the socket head screws (total 4ea) crossed by the
slanting lines to prevent emission monochromator alignment from distorting.

Thermo Scientific Lumina Service Manual 90

 17. Find each proper filter step position of the emission monochromator, using xenon lamp
beam and the ‘ Alignment’ software program.

18. Find each proper slit position of the emission monochromator, using the reflect mirror,
xenon lamp beam and the ‘Alignment’ software program.

19. Finally, make the monochromator wavelength recalibration referring to chapter

10. wavelength calibration.

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 VIII. Shutter Module Replacement

VIII-1. Shutter Module Replacement

Excitation side shutter Module Replacement Flow Chart

1) Start
2) Turn off the power of the instrument. And separate sample compartment.
3) Disassemble each housing part and cables.
4) Exchange the defected shutter module with a new shutter module.
And reassemble sample cell assay part and the upper case in reverse order.
5) End

Excitation side shutter Module Replacement Flow Chart

1) Start
2) Turn off the power of the instrument. And separate sample compartment.
3) Disassemble each housing part and cables.
4) Exchange the defected shutter module with a new shutter module.
And reassemble sample cell assay part and the upper case in reverse order.
5) End

Thermo Scientific Lumina Service Manual 92

 VIII-1-1. Excitation side shutter Module Replacement Procedure

1. Turn off the power of the instrument.

or

2. Remove white screw (total 10) located around on the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.

93 Lumina Service Manual Thermo Scientific

 4. Remove Phillips head screws (4 ea) located on the sample compartment using a
Phillips screwdriver.

5. Separate the sample compartment and lift the upper case.

Thermo Scientific Lumina Service Manual 94

 6. Separate the sample compartment.

7. Unscrew the Phillips head screws (2ea) located on the Shutter module and disconnect
the shutter module cable which is connected to the main board.

8. Exchange the defected shutter module with a new shutter module.

95 Lumina Service Manual Thermo Scientific

 9. Reassemble the sample compartment and the upper case in reverse order.

VIII-1-2. Emission side shutter Module Replacement Procedure

1. Turn off the power of the instrument.

or

2. Remove white screw (total 10) located around on the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.

Thermo Scientific Lumina Service Manual 96

 4. Remove Phillips head screws (4 ea) located on the sample compartment using a
Phillips screwdriver.

5. Separate the sample compartment and lift the upper case.

97 Lumina Service Manual Thermo Scientific

 6. Disconnect the shutter module cable which is connected to the main board. and
unscrew the Phillips head screws (2ea) located on the Shutter module.

7. Reassemble the sample compartment and the upper case in reverse order.

Thermo Scientific Lumina Service Manual 98

 IX. Power Switch Module Replacement

IX-1. Power Switch Module Replacement

Power Switch Module Replacement Flow Chart

1) Start
2) Turn off the power of the instrument and lit the upper case.
3) Unfasten the socket screw (2ea)
4) Disconnect the cables.
Exchange the defected power switch module with a new one.
5) End

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 IX-1-1. Power Switch Module Replacement Procedure

1. Turn off the power of the instrument.

or

2. Remove white screw (total 10) located around on the upper case.

3. Remove Phillips head screws using a Phillips screwdriver.

Thermo Scientific Lumina Service Manual 100

 4. Remove Phillips head screws (4 ea) located on the sample compartment using a
Phillips screwdriver.

5. Separate the sample compartment and lift the upper case.

101 Lumina Service Manual Thermo Scientific

 6. Unfasten the socket screw (2ea).

7. Disconnect the cables that correspond to power switch module from Terminal block,
Noise filter, Xenon lamp power, SMPS power supply.

8. Exchange the defected power switch module with a new power switch module

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 X. Wavelength Calibration

IX-1. Wavelength Calibration

Wavelength Calibration Flow Chart

1) Start
2) Turn off the power of the instrument.
3) Install Hg-Ar lamp to the instrument.
4) Make Emission Monochromator Calibration
5) Remove the Hg-Ar lamp from the instrument.
6) Place the Teflon Block into cell holder.
7) Make Excitation Monochromator Calibration.
8) End

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 The wavelength calibration of monchromators should be checked periodically. Especially
on monochromators where the ‘calibration’ is determined electronically rather than by
direct mechanical coupling. For calibration we use a low pressure Hg(mercury)-Ar (argon)
lamp with special accessories. This lamp provides a number of sharp lines which can be
used for calibration, and then measured Hg(mercury)-Ar (argon) wavelengths are
compared with the known values. A small slit width is used to increase precision of the
wavelength determination and to decrease the light intensity, which is important to
attenuate the light so that the photomultiplier tube and/or amplifiers are not damaged.

Hg(Mercury) line spectrum

60000
435.8 546.0

507.3
Relative Intensity(count)

40000

253.6
404.7
365.0
20000

576.9
313.2
296.5 579.0

200 400 600

Wavelength(nm)

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 60000 Ar(Argon) line spectrum

750.4
Relative Intensity(count) 50000
750.4

40000

696.5
30000
772.4 811.5
20000
826.4
738.4
794.8
10000
727.3 840.8
842.4
852.1
0

650 700 750 800 850 900

Wavelength(nm)

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 X-1-1. Emission Monochromator calibration

1. Prepare a low pressure Hg(mercury)-Ar (argon) lamp, a fiber optic and a fiber adaptor.

Hg-Ar calibration Lamp Fiber optic Fiber adaptor

(PN FS2-ACCE-03 ) (PN FS2-ACCE-04 ) (PN FS2-ACCE-05 )

2. Screw up the fiber adaptor to the emission monochromator lens holder.

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 3. Screw up the fiber optic to the fiber adaptor.

4. Turn on the power of the Hg (mercury)-Ar (argon) lamp.

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 This Lamp produces ultra violet radiation which can be harmful to the eyes. DO
NOT LOOK INTO THE LIGHT BEAM. THIS CAN CAUSE PERMANENT EYE
DAMAGE-WEAR PROTECTIVE EYE WEAR. This unit is supplied with a
mechanical safety device. Before removing this safety Device, be sure that the
power is off, and screw up the fiber optic cable before starting the instrument.

This Lamp lifetime is only about 50 hours. Therefore, you should always turn off
the power of the lamp after the calibration procedure is finished.

5. Run the ‘Alignment. EXE’ for calibration procedure located in the

Luminous Folder.

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 6. Setting each value on General tap window referring below table and when you can’t
measure spectrum peaks due to lamp intensity, adjust the PMT voltage.

Scan mode Emission

Data mode Luminescence

Hg:200-500V,
PMT voltage
Ar: 500-700V

EM Slit width 1nm

Hg: Air
EM Filter
Ar: 530nm

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 7. Find the zeroth order step value of the Emission monochromator to search the
start step position of the Hg spectrum easily. Commonly the start step position of the
Hg spectrum is displayed after about 2000 step from the zeroth order step.

zeroth order The start step position of the Hg spectrum

To know the zeroth order step of emission monochromator, first check the grating
position.
Monochromator has zeroth order when gratin position is parallel to case wall.
To rotate the grating, use the grating mode of Alignment software .

① Click limit and grating will move to start position.

② Enter proper step value you want and click the arrow mark.

③ Grating will move corresponding to entered step value. And moved step value is

displayed on window.
④ When grating position is parallel to the case wall, record the displayed step value.

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 3
1
2

Tip: You can find the approximate zeroth order step referring to the
‘Alignment.ini’ file located in the Luminous Folder.

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 8. Zoom in each step’s peaks value of the Hg spectrum one by one and record each
step’s peaks value comparing to the known peak values of Hg spectrum wavelengths.

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 9. After all the step value peaks of the Hg spectrum were recorded, move to the Ar
spectrum calibration procedures.
Change Air status filter to 530nm filter and then set PMT voltage value about 500V
(300V=>500V) the reason of the change from Air to 530nm filter is to get rid of the
Hg spectrum line interference.
The reason of increasing PMT voltage value is to increase Ar spectrum intensity since
the 530nm filter optical density decrease Ar spectrum intensity.

10. Check the combo box of view grating step and enter proper step value in the EM start
Step nearby the end of Hg spectrum step value and then click Start .

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 11. Zoom in each step’s peaks value of the Ar spectrum one by one and record each step’s
peaks value comparing to the known peak value to the Ar spectrum wavelengths

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 12. After all the step’s peaks values of the Ar spectra were recorded, Input each step value
that corresponds to the proper wavelength in the table following the Ar spectrum step
value, and then click calibration.

13. After all step value peaks of Hg & Ar spectrum were recorded in the table,
Click calibration, and then the ‘Curve fitting is OK’ window is displayed.

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 14. After clicking Curve fitting is OK, the wavelength calibration result is displayed and
then click OK and save.

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 15. Emission Monochromator calibration is finished and the file related to the calibration is
saved in the following folder: C:\Program Files\Luminous\Calibration\Emission wave.
Finally open the emission wave file and the Emission Center_Band.ini file and check
whether there is any problem or not.

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 X-1-2. Excitation Monochromator calibration

After the calibration of the emission monochromator, the excitation monochromator can be
calibrated with a new standard. The slit width of both the emission monochromator and the
excitation and should be set the same small value, consistent with the available light
intensity a dilute suspension of glycogen is placed in the cell holder to scatter the exciting
light. The emission monochromator is set to a certain wavelength. If the excitation
monochromator is properly calibrated, then the maximum intensity of the scattered light is
seen when the indicated wavelength is identical.

1. Prepare the Teflon block. (PN FS2-ACCE-08)

2. Place the Teflon block into the cell holder in the sample compartment.

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 3. Click xenon lamp, and then check whether xenon lamp is on.

4. Click New folder and save it with proper file name when save window is displayed

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 5. Click OK.

6. Select ExCalibration Scan mode and click Start.

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 7. The excitation step value is measured automatically and you can find step marking
combo box. Insert step value corresponding from 200nm to 850nm into table in order of
precedence, click save and then, click calibration.

8. After clicking curve fitting is OK, wavelength calibration result is displayed. And then
click OK and Save.

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 9. Excitation Monochromator calibration is finished and the file related to the
Calibration is saved in the following folder: C:\Program Files\Luminous\Calibration
\Excitation wave. Finally open the emission wave file and the Excitation
Center_Band.ini file and check whether there is any problem.

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 XI. Validation

Validation Flow Chart

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 XI-1. Sensitivity Test – Water Raman

XI-1-1. Sensitivity Test Method

1. Turn on the main power switch on the rear panel of the system.

2. Turn on the sub main power switch at the bottom right side of the front panel of the
system.

3. Push the Light Source button (Red) to turn on light firstly and push the Control Board
button (White) to turn on the main board.

caution
- It is important to keep up the order during turning powers on:
#1 Main P/S Æ #2 Light Source Æ #3 Control Board
- If the control board is powered on at first, it could cause damage to the
electric board by the high voltage/current when the light source is on later.
- To turn off the system, you need to follow the procedures in reverse order
against the start up. (#3 Control Board Æ #2 Light Source Æ #1 Main P/S)
- Before turning off the main power switch, you need to wait for over 10 minutes
at least to protect the lifetime of lamp after turned off the light source switch.

4. Check whether all three LED lights at the left side of the front panel of the system are lit
on. If it is ready, warm up the system for about 20-30 minutes.

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 5. Run the validation software in the Luminous folder C:\Program Files\Luminous
Software.

6. Click New and Save after naming proper file name.

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 7. Click OK.

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 8. Click Water Raman tab and click Start.

9. The ‘Fill cell with distilled water and set the cell in the holder’ message will be displayed.
Put the cuvette cell containing distilled water (HPLC grade) in the cell holder. And close
the lid and click OK.

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Thermo Scientific Lumina Service Manual 128
 10. After measurements of the Raman and noise signals, the S/N ratios of Peak to Peak
and RMS will be evaluated.

11. The calculated RMS S/N ratio should be greater than 4000:1 and the Peak to Peak
S/N ratio should be greater than 1000:1. If the S/N ratio value is lower than the
acceptance value, refer to ‘Suspected Malfunction’ in chapter XI-1-2. Instrument
Condtion.

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 XI-1-2. Instrument Condition

▶ Instrument Condition

General
Scan mode Emission
Data mode Fluorescence
Repeat Number 1
Start Delay 0
Repeat Interval Time 1
PMT Voltage 700
Scan Speed (nm/min) 300
Integration Time (ms) 100
Response Time (s) 0.1
Average Number 1
EX Slit (nm) 10
EM Slit (nm) 10
EX Filter Air
EM Filter Air
Wave Setup
EX Wave (nm) 350
EM Start (nm) 360
EM End (nm) 460
EM Interval (nm) 1
Peak Check Position(nm) 397(nm)
Time Setup
Interval time (ms) 100
Total time (ms) 60000 (1min)

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 ▶ Suspected malfunction

After turning on the power of the

The lamp intensity is not stably instrument, warming up at least
lit 30 minutes until the intensity is
stable

Xenon lamp is aged Replace as a new Xenon lamp.

S/N ratio does Contaminated distilled water or

a dirty cuvette cell might result
not satisfy the
in the increased background
guaranteed value noise owing to scattered light or
Distilled water
impurity-induced fluorescence,
is contaminated
eventually decreasing the S/N
ratio. Rinse the cuvette cell
thoroughly. Use clean distilled
water only.

Contact nearest sales

Other causes
representative

▶ Acceptance

Peak to Peak S/N ratio: greater than 1000

RMS S/N ratio: greater than 4000
Status
Specifications Results
Peak to Peak (Pass/Fail)
S/N Ratio
Greater than1000

Status
Specifications Results
RMS (Pass/Fail)
S/N Ratio
Greater than 4000

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 XI-2. Emission wavelength Accuracy Test(1)_
Mercury(Hg) Line

XI-2-1. Emission wavelength Accuracy Test(1) Method

1. Prepare Hg-Ar lamp and optical fiber modules.

2. Plug the fiber mount to the Emission lens holder.

3. Plug the optical fiber to the fiber mount by fixing the SMA connector.

4. Turn on the Hg-Ar lamp power supply.

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 5. Click EM Wave Test(Hg) tab and click Start in the Validation software.

6. If the message of readjusting EM wavelength is shown, click Yes to pass the

wavelength test.

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 XI-2-2. Instrument Condition

▶ Instrument Condition

General
Scan mode Emission
Data mode Luminescence
PMT Voltage Automatic
Scan Speed (nm/min) User defined
Integration Time (ms) 20
Response Time (s) 0.02
Average Number 1
EX Slit (nm) 1
EM Slit (nm) 1
EX Filter (nm) Air
EM Filter (nm) Air
Wave Setup
EX Wave (nm) Not applied
EM Start (nm) 240
EM End (nm) 450
EM Interval (nm) 0.1

▶ Acceptance

Deviation of measured wavelength from the certified lamp wavelength should be less
than ±0.5 nm.
Lamp Wavelength Measured
Deviation(nm) Status(Pass/Fail)
(nm) Wavelength(nm)
253.7
435.9

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 XI-3. Emission wavelength Accuracy Test(2)_
Argon(Ar) Line

XI-3-1. Emission wavelength Accuracy Test(2) Method

1. Prepare Hg-Ar lamp and optical fiber modules.

2. Plug the fiber mount to the Emission lens holder.

3. Plug the optical fiber to the fiber mount by fixing the SMA connector.

4. Turn on the Hg-Ar lamp power supply.

5. Click EM Wave Test(Ar) tab and click Start in the Validation software.

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 6. If the message of readjusting EM wavelength is shown, click Yes to pass the
wavelength test.

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 XI-3-2. Instrument Condition

▶ Instrument Condition

General
Scan mode Emission
Data mode Luminescence
PMT Voltage(volt) Automatic
Scan Speed(nm/min) User defined
Integration Time(ms) 20
Response Time(s) 0.02
Average Number 1
EX Slit (nm) 1
EM Slit (nm) 1
EX Filter(nm) Air
EM Filter (nm) 530
Wave Setup
EX Wave(nm) No applied
EM Start(nm) 650
EM End(nm) 800
EM Interval(nm) 0.1nm

▶ Acceptance

Difference between lamp wavelength and measured wavelength should be less than
±0.5 nm.
Lamp Wavelength Measured
Deviation(nm) Status(Pass/Fail)
(nm) Wavelength(nm)
696.5
794.8

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 XI-4. Excitation wavelength Accuracy Test

XI-4-1. Excitation wavelength Accuracy Test Method

1. Prepare Teflon Block.

2. Plug the single cell holder to the sample compartment.

3. Put the Teflon Block into the single cell holder.

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 4. Click Ex Wave Test (Teflon) tab and click Start in the Validation software.

5. If the message of readjusting EX wavelength is shown, click Yes to pass the

Wavelength test.

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 XI-4-2. Instrument Condition

▶ Instrument Condition

General
Scan mode Excitation
Data mode Fluorescence
PMT Voltage(volt) Automatic
Scan Speed(nm/min) User defined
Integration Time(ms) 20
Response Time(s) 0.02
Average Number 1
EX Slit (nm) 1
EM Slit (nm) 1
EX Filter(nm) Air
EM Filter (nm) Air
Wave Setup
EM Wave(nm) 300nm, 400nm, 500nm, 600nm
EX Start(nm) -10nm at each EM Wave
EX End(nm) +10nm at each EM Wave
EX Interval(nm) 0.1nm

▶ Acceptance

Deviator of measured excitation wavelength from assigned wavelength should be less

than ±0.5 nm.

Emission Measured Ex.

Deviation(nm) Status(Pass/Fail)
Wavelength(nm) Wavelength(nm)

300
400
500
600

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 XI-5. Excitation wavelength Accuracy Test and
Repeatability Test-0nm

XI-5-1. Excitation wavelength Accuracy Test and Repeatability Test Method

1. Prepare Teflon Block.

2. Plug the single cell holder to the sample compartment.

3. Put the Teflon Block into the single cell holder.

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 4. Click Ex Wave Test (zero, Teflon) tab and click Start in the Validation software.

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 XI-5-2. Instrument Condition
▶ Instrument Condition

General
Scan mode Excitation
Data mode Fluorescence
PMT Voltage(volt) Automatic
Scan Speed(nm/min) User defined
Integration Time(ms) 20
Response Time(s) 0.02
Average Number 1
EX Slit (nm) 1
EM Slit (nm) 1
EX Filter(nm) Air
EM Filter (nm) Air
Wave Setup
EM Wave(nm) 0nm
EX Start(nm) -10nm
EX End(nm) +10nm
EX Interval(nm) 0.1nm

▶ Acceptance

Deviation of measured excitation wavelength from assigned wavelength should be less

than ±0.5 nm. And (Maximum-Minimum)/2 should be less than 0.2 nm.

Emission Measured Ex.

Deviation(nm) Status(Pass/Fail)
Wavelength(nm) Wavelength(nm)

0
0
0
0
0
Maximum-
Status(Pass/Fail)
Minimum (nm)

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 XI-6. Emission wavelength Accuracy Test and
Repeatability Test-0nm

XI-6-1. Emission wavelength Accuracy and Repeatability Test Method

1. Prepare Teflon Block.

2. Plug the single cell holder to the sample compartment.

3. Put the Teflon Block into the single cell holder.

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 4. Click EM Wave Test (zero, Teflon) tab and click Start in the Validation software.

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 XI-6-2. Instrument Condition
▶ Instrument Condition

General
Scan mode Emission
Data mode Fluorescence
PMT Voltage(volt) Automatic
Scan Speed(nm/min) User defined
Integration Time(ms) 20
Response Time(s) 0.02
Average Number 1
EX Slit (nm) 1
EM Slit (nm) 1
EX Filter(nm) Air
EM Filter (nm) Air
Wave Setup
EX Wave(nm) 0nm
EM Start(nm) -10nm
EM End(nm) +10nm
EM Interval(nm) 0.1nm

▶ Acceptance

Deviation of measured excitation wavelength from assigned wavelength should be less

than ±0.5 nm. And (Maximum-Minimum)/2 should be less than 0.2 nm.

Excitation Measured EM.

Deviation(nm) Status(Pass/Fail)
Wavelength(nm) Wavelength(nm)

0
0
0
0
0
Maximum-
Status (Pass/Fail)
Minimum (nm)

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 XI-7. Reporting Validation Result
XI-7-1. Print Report Cover

1. Click Print Report Cover in the File menu.

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 2. If you want to change the logo image, click Insert Company Logo and select the file
you want to import.

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 3. You can the image is changed.

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 XI-7-2. Print Validation Report

1. Click Print Validation Report in the File menu.

2. The following window will be shown. Click Print.

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 XI-7-3. Print Detailed Validation Results
1. Click Print EM Wave Test (Hg) in the File menu.

2. You can print the each detailed test result. The other detailed test results can be
printed out in the same way.

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 XII. Correction Spectra

XII-1. Correction Spectra

Correction Spectra Flow Chart

1) Start
2) Turn on the power of the instrument.
3) Adapt the Standard Lamp to the instrument.
4) Generating Emission Correction Factors.
5) Remove the Standard Lamp from the instrument.
6) Place Rhodamine B (Standard quantum counter) in the cell holder.
7) Generating Excitation Correction factors.
8) End

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 The development of methods to obtain excitation and emission spectra, corrected for
wavelength –dependent effects, has been the subject of numerous investigations.
Spectrum correction is performed to make it possible to measure a true spectrum by
eliminating instrumental response needed to perform spectrum correction. ‘

XII-1-1. Generating emission correction factors

One way to generate emission correction factors for your instrument is to scan the
spectrum of a standard lamp. Because the actual irradiance values of the standard lamp
over wavelength are known, dividing the irradiance values by the lamp spectrum results in
a set of relative correction values.

1. Prepare a Standard Lamp set including an optical fiber and a reflect mirror adaptor.

2. Place the reflect mirror adaptor in the cell holder in the sample compartment.

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 3. Turn on the Standard lamp (push the Red color button HALOGEN) and warming it up
about 20 minutes.

4. Run the Wave Scan mode.

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 5. Create a new file name and save it.

6. Click OK when the ‘Create new experiments’ window is displayed. And then the correct
spectra icon is activated.

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 7. Select proper Emission Slit width, and fix Emission filter state to air state. Commonly,
Emission Slit width is 5 nm.
After slit and filter position settings are completed click Correct Spectra.

8. The Correct Spectra window is displayed.

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 9. Input the proper PMT measurement voltage and PMT Integration time.
Normally, the proper PMT voltage is 500 (V).
Click Correct Spectra (Standard W Lamp).

If the measured intensity of standard lamp is saturated as below [Saturated Intensity

picture], decrease PMT voltage value and measure again until measured Standard
Lamp max peak intensity is within the Y axis maximum value (65,355 counts). [Closer
intensity to Y axis maximum picture]

Saturated Intensity Closer intensity to y axis maximum

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 10. The measured intensity of standard lamp is within the Y axis maximum value, click OK
on the window.

11. After Standard lamp measurement procedure is finished, go to the next step
‘Generating excitation correction factors’ using Rhodamine B 9 Standard quantum
counter).

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 XII-1-2. Generating excitation correction factors

One way to generate excitation correction factors for your instrument is to scan the
spectrum of Rhodamine B (Standard quantum counter). Because the actual measured
emission values of the Rhodamine B over wavelength are known, dividing the measured
values by the Rhodamine B spectrum results in a set of relative correction values.

1. Click Correct Excitation Spectra (Rhodamine B) and then click OK when the ‘Please
put the Rhodamine B in triangular cell into the cell holder’ window is displayed

2. Prepare Rhodamine B (Standard quantum counter), fill the prepared Rhodamine B in

the triangular cell and place it in the sample holder located in the sample compartment.

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 Rhodamine B (Standard quantum counter) is filled in triangular cell
Pay attention to the setting direction of Triangular cell in the cell holder. The setting
direction of Triangular cell (top view) is shown as below.

Excitation light

Emission light

Setting Direction of Triangular Cell (Top view)

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 3. Input the proper PMT measurement voltage and PMT Integration time.
Commonly, the proper PMT voltage is 500 (V).
Click Correct Excitation Spectra (Rhodamine B).

If the measured intensity of Rhodamine B (Standard quantum counter) is saturated,

decrease PMT voltage value and measure again until Rhodamine B (Standard
quantum counter) max peak intensity is within the Y axis maximum value (65,355
counts).[Closer intensity to Y axis maximum picture]

Closer intensity to Y axis maximum

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 4. The measured intensity of Rhodamine B (Standard quantum counter) is within the Y
axis maximum value, click OK on the window, click Save change, and then, click Save
Change finally.

The result of Excitation correction factor is saved as the ‘correctionFactor_EX.ini’ file in

the C:\Program Files\Luminous\Calibration folder.

The result of Emission correction factor is saved as the ‘correctionFactor_EM.ini’ file in

the C:\Program Files\Luminous\Calibration folder.

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 The result of
Standard Lamp Intensity Spectrum

The result of
Rhodamine Intensity Spectrum

The result of
Excitation correction factor. The result of
Emission correction Factor.

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 XIII. Diagnostic Software

XIII-1. Diagnostic Software

Diagnostic Software Flow Chart

1) Start
2) Turn on the power of the instrument and place the Teflon block into the cell holder.
3) Run Diagnostics software.
4) Check the check/uncheck boxes of items to be tested and enter the proper repeat
number. (commonly 2-5)
5) Click Start.
6) End

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 XIII-1-1. How to execute Diagnostics Software.

1. Turn on the main power switch on the rear panel of the system.

2. Turn on the sub main power switch at the bottom right side of the front panel of
the system.

3 2 1

3. Push the Light Source button (Red) to turn on light firstly and push the Control
Board button (White) to turn on the main board.

Caution!
- It is important to keep up the order during turning powers on:
#1 Main P/S Æ #2 Light Source Æ #3 Control Board
- If the control board is powered on at first , it could cause damage to the electric
board by the high voltage/current when the light source is on later.
- To turn off the system, you need to follow the procedures in reverse order against
the start up. (#3 Control Board Æ #2 Light Source Æ #1 Main P/S)
- Before turning off the main power switch, you need to wait for over 10 minutes at
least to protect the lifetime of lamp after turned off the light source switch.

4. Check whether all three LED lights at the left side of the front panel of the system
are lit on. If it is ready, warm up the system for about 20-30 minutes.

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 5. Run ‘Diagnostics’ in the Luminous Software folder on Desktop.

6. Click OK after ‘Reading serial number: OK’ window is displayed.

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 7. You can check the serial number of instrument shown as below.

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 8. Select the items which you want to inspect and enter the proper repeat numbers in
Repeat Number boxes on the Main Controller Test panel. For the initial inspection,
all items are recommended to be checked and the proper repeat number is ‘2-5’.

9. Click Start.

10. You will see the message.

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 11. Place the Teflon block into the cell holder, and then click OK.

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 12. And the total diagnostic progress status and the progress status of each
component will be shown.

Status of total
diagnostic progress

Progress status of
each component

Checked items
will be tested

13. If the diagnostic passes the test, then the following message will be shown.

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 14. If each component has no problem, the test result will be displayedas ‘Pass’.
If any component has a problem, the result will be displayed as ‘Fail’.
In this case, you need to stop testing the diagnostic on the failed items.

15. If the diagnostic fails the test, the following message will be shown. If you did not
recalibrate the wavelength in Alignment.exe, recalibrate both of monochromator
using Alignment.exe.

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 16. Click Print to print out the result of diagnostics.

17. Click Help to search the items to be defined.

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 XIII-2. Diagnostics Error Chart

Error Cause Corrective action

Poor cable connection Check RS-232 Cable connection

Check Computer COM port and

Computer COM port failed connect with other computer for
Main Board Failed the cross-checking

Connect with other computer for

Instrument COM port failed
the cross-checking

Bad Main controller in the

Replace Main board
board
Xenon lamp is not turned
Excitation Slit Failed Run the diagnostic software
on.
Recalibrate the wavelength
Wavelength is shifted
using Alignment.exe

Open the upper cover and check

the Excitation Slit positions
Excitation Slit positions are
If it is misaligned, Recalibrate
not correct
the slit positions using Alignment
software

Open the upper cover and check

the Excitation Filter positions.
Excitation Filter positions
If it is misaligned, Recalibrate
are not correct
the Filter positions using
Alignment software

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 Open the upper cover and check
the Excitation monchromator
Excitation Monochromator position. If it is misaligned,
is not correct Recalibrate the monochromator
position using Alignment
software

Error Cause Corrective action

Detector cannot receive Check the cable connection or

the signal Replace PD board
Excitation Slit Failed
Start position is different Limit switch could be
every time or Slit Wheel defected.

rotates continuously Replace the limit switch

Xenon lamp is not turned

Excitation Filter Failed Run the diagnostic software
on
Open the upper cover and check
the Excitation Filter positions.
Excitation Filter positions
If it is misaligned, Recalibrate
are not correct the Filter positions using
Alignment software

Open the upper cover and check

the Excitation Slit positions.
Excitation Slit positions are
If it is misaligned, Recalibrate
not correct the slit positions using Alignment
software

Open the upper cover and check

the Excitation monchromator
Excitation Monochromator position. If it is misaligned,
is not correct Recalibrate the monochromator
position using Alignment
software

Detector cannot receive Check the cable connection or

the signal Replace PD board

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 Start position is different Limit switch could be
every time or Slit Wheel defected.

rotates continuously Replace the limit switch

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 Error Cause Corrective action

Xenon lamp is not turned

Run the diagnostic software
on

Open the upper cover and check

the Excitation monchromator
Excitation monochromator position. If it is misaligned,
is not correct Recalibrate the monochromator
position using Alignment
software

Open the upper cover and check

the Excitation Slit positions.
Excitation Slit positions are
If it is misaligned, Recalibrate
not correct the slit positions using Alignment
Excitation software
Monochromator Failed
Open the upper cover and check
the Excitation Filter positions.
Excitation Filter positions
If it is misaligned, Recalibrate
are not correct the Filter positions using
Alignment software

Detector cannot receive Check the cable connection or

the signal Replace PD board

Start position is different Limit switch could be

every time or Slit Wheel defected

rotates continuously Replace the limit switch

Thermo Scientific Lumina Service Manual 176

 Error Cause Corrective action

Xenon lamp is not turned

Run the diagnostic software
on

Teflon Block is not Put the Teflon Block into cell

installed into the cell holder holder

Open the upper cover and check

the Emission Slit positions. If it is
Emission Slit positions are
misaligned, Recalibrate the slit
not correct
positions using Alignment
software
Open the upper cover and check
the Emission Filter positions. If it
Emission Filter positions
is misaligned, Recalibrate the
are not correct
Filter positions using Alignment
Emission Slit Failed
software
Open the upper cover and check
the Emission monchromator
Emission Monochromator position. If it is misaligned,
is not correct Recalibrate the monochromator
position using Alignment
software
Start position is different Limit switch could be
every time or Slit Wheel defected.

rotates continuously Replace the limit switch

Detector cannot receive Check the PMT detector and

the signal cable connection

Shutters are not opened Refer to ‘shutter check is failed’

177 Lumina Service Manual Thermo Scientific

 Error Cause Corrective action

Xenon lamp is not turned

Run the diagnostic software
on
Teflon Block is not Put the Teflon Block into cell
installed into cell holder holder
Open the upper cover and check
the Emission Filter positions. If it
Emission Filter positions
is misaligned, Recalibrate the
are not correct
Filter positions using Alignment
software
Open the upper cover and check
the Emission Slit positions. If it is
Emission Slit positions are
misaligned, Recalibrate the slit
not correct
positions using Alignment
Emission Filter Failed software
Open the upper cover and check
the Emission monchromator
Emission Monochromator position. If it is misaligned,
is not correct Recalibrate the monochromator
position using Alignment
software
Start position is different Limit switch could be
every time or Slit Wheel defected.

rotates continuously Replace the limit switch

Detector cannot receive Check the PMT detector and

the signal cable connection

Shutters are not opened Refer to ‘shutter check is failed’

Thermo Scientific Lumina Service Manual 178

 Error Cause Corrective action

Xenon lamp is not turned

Run the diagnostic software
on

Teflon Block is not Put the Teflon Block into cell

installed into cell holder holder

Open the upper cover and check

the Emission monchromator
Emission Monochromator position. If it is misaligned,
is not correct Recalibrate the monochromator
position using Alignment
software
Open the upper cover and check
the Emission Slit positions. If it is
Emission Slit positions are
Emission misaligned, Recalibrate the slit
not correct
Monochromator Failed positions using Alignment
software
Open the upper cover and check
the Emission Filter positions. If it
Emission Filter positions
is misaligned, Recalibrate the
are not correct
Filter positions using Alignment
software
Start position is different Limit switch could be
every time or Slit Wheel defected.

rotates continuously Replace the limit switch

Detector cannot receive Check the PMT detector and

the signal cable connection

Shutters are not opened Refer to ‘shutter check is failed’

179 Lumina Service Manual Thermo Scientific

 Error Cause Corrective action

Check the solenoid valve is

Excitation Shutter Check working properly, cable
Shutter is not opened
Failed connection and for Input
voltage(12V) with test meter

Check the solenoid valve is

Emission Shutter Check working properly, cable
Shutter is not opened
Failed connection and for Input
voltage(12V) with test meter

Thermo Scientific Lumina Service Manual 180

 XIV. Firmware Upgrade
This document is made for helping firmware updating
conveniently.

Commonly the firmware updating of main-board is necessary when the

function of instrument and the function of accessories are added or changed.

The firmware updation of accessories ard added or changed

(e.g Fast Filter, multi cell, rapid mix and so on.)

XIV-1. Updating the firmware of main board

XIV-1-1. Installation of the Firmware Update Utility for main B/D update

1. Unzip FW_Downloader.zip file.

2. Double click Setup.exe in the FW Downloader folder.

181 Lumina Service Manual Thermo Scientific

 3. The following window is displayed for installing NET Framework 2.0. Click Accept.

4. NET Framework 2.0 is installed, If NET Framework 2.0 already is installed, go to step 5,
skipping step 3 and4.

Thermo Scientific Lumina Service Manual 182

 5. Firmware Update Utility is executing after Net Framework 2.0 installation is completed.

6. Click Install when below figure is displayed.

7. Firmware Update utility installation is finished.

183 Lumina Service Manual Thermo Scientific

 XIV-2. Updating the firmware of main B/D using update
utility software

1. Turn off control board power and light source power.

2. Connect the instrument with PC where the Firmware update utility software is installed
using RS-232 communication cable.

3. Execute Firmware Update Utility.

Thermo Scientific Lumina Service Manual 184

 4. Load the file corresponding firmware updating, there are two method; one is usage of
file/open, the other method is usage of load file. (Refer to ① and the other is ②.)

5. If firmware update installation is completed, the following message is displayed.

185 Lumina Service Manual Thermo Scientific

 Caution:
Check whether COM-Port number of firmware downloader software is same with
the COM-Port of instrumentation. For perfect updating the COM-Port of firmware
update utility software must same with the COM-Port of instrumentation.

6. Click

Thermo Scientific Lumina Service Manual 186

 7. Push Main P/S and control board switch in the front side of the instrument.

Caution:
Turn off the Light source the noise from the light source may influence on the
firmware update failure.)

Caution:
Don’t turn off the main power during the firmware updating.

8. The progress is displayed when the power is turned on.

187 Lumina Service Manual Thermo Scientific

 9. If all firmware update is completed successfully, the completion message is displayed
as below.

Thermo Scientific Lumina Service Manual 188

 10. If all firmware update is finished, turn off the power and close firmware update utility.

189 Lumina Service Manual Thermo Scientific

 XIV-3. Replacement of Accessory B/D

1. Turn off power of the instrument and disconnect the power cable.

2. Remove white screw caps (total 10 ea) and Phillips head screws, and then lift up the
upper case.

Thermo Scientific Lumina Service Manual 190

 3. Separate the accessory connector socket module from the sample compartment, lifting
up the sample compartment.

4. Pull out the Flash Rom using IC extractor.

191 Lumina Service Manual Thermo Scientific

 5. Insert a new Flash ROM into the socket.

Caution:
Give attention to Pin array direction.

6. If all Flash ROM replacement is completed, reassemble the sample compartment and
the upper case in reverse order.

Thermo Scientific Lumina Service Manual 192

 XV. Troubleshooting
Lumina Fluorescence spectrometer system was designed to operate reliably and
predictably. If the instrument has a problem, take measures by referring to the table
below. When a symptom in question is not found in the table, contact Thermo
Scientific or sales representative.

Problem Possible Cause Remedial action

Securely insert the plugs at both ends of

Power cable is not securely
the cable into the inlet on the instrument
1. Instrument is not powered connected.
and the outlet at the site.
though the power switch is
in the ON position Power fuse is blown. Replace the fuse with a new one.

Contact Thermo Scientific or sales

Other causes.
representative

193 Lumina Service Manual Thermo Scientific

 Shutter is in the closed
position, or the slit on the Using software, open the shutter or the
emission side is in the slit.
‘Close’ status

Slits are not opened to the Adjust the slits to proper width
proper width. Using

2. Light is not reaching the Refer to 3. ‘Lamp is not turned on’

Lamp is not lit
problem below.
sample

Check and recalibrate monochromators. If

Monochromator is the problem is not solved after the
miscalibrated monochromator recalibration, Contact
SCINCO or sales representative

Accessory is not a right

Relocate the position
position.

Check the connection of the high-voltage

lead on the positive (+) electrode side of
the lamp.
Before opening the cover of the lamp
Wiring is disconnected
house, be sure to disconnect the power
3. Lamp is not turned on cable from outlet. Inadvertently powering
ON of the instrument with the cover open
is extremely dangerous.

Allow the lamp to cool down for about ten

Lamp is still hot
minutes.

After turning on the power of the

Xenon lamp is not stably lit instrument, wait for at least 30 minutes
until the Xenon lamp is stably lit.

Xenon lamp is aged Replace Xenon lamp.

4. S/N ratio does not satisfy
the guaranteed value Contaminated distilled water or dirty
cuvette cell will result in the increased
background noise owing to scattered light
Distilled water is
or impurity-induced fluorescence,
contaminated.
eventually decreasing the S/N ratio. Rinse
the cuvette cell thoroughly. Use clean
distilled water only.

5. Signal does not come out The Xenon lamp is

Position lamp correctly
misaligned

Thermo Scientific Lumina Service Manual 194

 Wrong acquisition Correctly specify the wavelength, slit
parameters width , filter etc.

Shutter is in the closed

position, or the slit on the
Using software, open the shutter or slit.
emission side is in ‘Close’
status

Refer to 3. ‘Lamp is not turned on’

Lamp is unlit
symptom above.

Reduce slit setting or adjust PMT voltage

Data is saturated
setting properly

Lamp is not turned on Run the Diagnostic software.

6. Abnormal spectra results
Light leaks Check dark value to determine.

IR sensors in a
7. Hardware Initializing error monochromator, a slit and a Replace the IR sensor
filter could be broken

195 Lumina Service Manual Thermo Scientific