OPERATING MANUAL

ba77147e01 07/2016

Pro
Lab
250
0

ProLab 2500
DIGITAL METER FOR IDS SENSORS
 ProLab 2500

Copyright © 2016 Xylem Analytics Germany GmbH

Printed in Germany.

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ProLab 2500 Contents

ProLab 2500 - Contents

1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Meter ProLab 2500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2.1 IDS sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2.2 IDS adapter for analog sensors . . . . . . . . . . . . . . . . . . . . . 9
1.2.3 Automatic sensor recognition . . . . . . . . . . . . . . . . . . . . . . 9

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1 Safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.1 Safety information in the operating manual . . . . . . . . . . . 10
2.1.2 Safety signs on the meter . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.3 Further documents providing safety information . . . . . . . 10
2.2 Safe operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2.1 Authorized use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2.2 Requirements for safe operation . . . . . . . . . . . . . . . . . . . 11
2.2.3 Unauthorized use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.2 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3.1 Connecting the power pack . . . . . . . . . . . . . . . . . . . . . . . 13

4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.1 General operating principles . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.1.1 Keypad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.1.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1.3 Status information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1.4 Instrument connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.1.5 Channel display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1.6 Sensor info. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1.7 Display of several sensors in the measuring mode .. 18
4.2 Switching on the meter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.3 Switching off the meter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.4 Login with user name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.5 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.5.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.5.2 Measured value display . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.5.3 Menus and dialogs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.5.4 Example 1 on navigation: Setting the language . . . . . . . 23
4.5.5 Example 2 on navigation: Setting the date and time . . . . 25

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Contents ProLab 2500

5 pH value. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.1 Measuring the pH value . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.2 Measuring the temperature . . . . . . . . . . . . . . . . . . . . . . 28
5.2 pH calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.2.1 Why calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.2.2 When do you have to calibrate? . . . . . . . . . . . . . . . . . . . 29
5.2.3 Carrying out automatic calibration (AutoCal) .... 29
5.2.4 Carrying out a manual calibration (ConCal) . . . . . . . . . . 32
5.2.5 Calibration points . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.2.6 Calibration data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.2.7 Continuous measurement control (CMC function) .. 38
5.2.8 QSC function (sensor quality control) ............ 40

6 ORP voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
6.1 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
6.1.1 Measuring the ORP . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
6.1.2 Measuring the relative ORP . . . . . . . . . . . . . . . . . . . . . . 44
6.1.3 Measuring the temperature . . . . . . . . . . . . . . . . . . . . . . 46
6.2 ORP calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

7 Ion concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.1 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.1.1 Measuring the ion concentration . . . . . . . . . . . . . . . . . . 47
7.1.2 Measuring the temperature . . . . . . . . . . . . . . . . . . . . . . 49
7.2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2.1 Why calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2.2 When to calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2.3 Calibration (ISE Cal). . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2.4 Calibration standards . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.2.5 Calibration data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3 Selecting the measuring method . . . . . . . . . . . . . . . . . . . . . . . 56
7.3.1 Standard addition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
7.3.2 Standard subtraction . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
7.3.3 Sample addition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
7.3.4 Sample subtraction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
7.3.5 Standard addition with blank value correction (Blank value
addition) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

8 Dissolved oxygen (D.O.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

8.1 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
8.1.1 Measuring D.O. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
8.1.2 Measuring the temperature . . . . . . . . . . . . . . . . . . . . . . 71
8.2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.2.1 Why calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.2.2 When to calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.2.3 Calibration procedures . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.2.4 Calibration data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

9 Conductivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
9.1 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

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9.1.1 Measuring the conductivity . . . . . . . . . . . . . . . . . . . . . . . 74

9.1.2 Measuring the temperature . . . . . . . . . . . . . . . . . . . . . . 76
9.2 Temperature compensation . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
9.3 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
9.3.1 Why calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
9.3.2 When to calibrate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
9.3.3 Determining the cell constant (calibration in control
standard) ................................ 77
9.3.4 Calibration data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

10 Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
10.1 pH measurement settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
10.1.1 Settings for pH measurements . . . . . . . . . . . . . . . . . . . . 80
10.1.2 Buffer sets for calibration . . . . . . . . . . . . . . . . . . . . . . . . 82
10.1.3 Calibration interval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
10.2 ORP measurement settings. . . . . . . . . . . . . . . . . . . . . . . . . . . 85
10.3 ISE measurement settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
10.4 D.O. measurement settings . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
10.4.1 Settings for D.O. measurement . . . . . . . . . . . . . . . . . . . 88
10.5 Cond measurement settings . . . . . . . . . . . . . . . . . . . . . . . . . . 88
10.5.1 Settings for IDS conductivity sensors . . . . . . . . . . . . . . . 88
10.6 Sensor-independent settings . . . . . . . . . . . . . . . . . . . . . . . . . . 91
10.6.1 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
10.6.2 Data storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
10.6.3 Automatic Stability control . . . . . . . . . . . . . . . . . . . . . . . 92
10.7 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
10.7.1 Resetting the measurement settings . . . . . . . . . . . . . . . 93
10.7.2 Resetting the system settings. . . . . . . . . . . . . . . . . . . . . 95

11 Data storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
11.1 Manual data storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
11.2 Automatic data storage at intervals . . . . . . . . . . . . . . . . . . 96
11.3 Measurement data storage . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
11.3.1 Editing the measurement data storage. . . . . . . . . . . . . . 99
11.3.2 Erasing the measurement data storage . . . . . . . . . . . . 100
11.3.3 Measurement dataset. . . . . . . . . . . . . . . . . . . . . . . . . . 100
11.3.4 Storage locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

12 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

12.1 Outputting current measurement data . . . . . . . . . . . . . . . . . . 102
12.2 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
12.3 Connecting the PC / USB-B interface (USB Device) .... 102
12.4 Connecting a USB memory device/USB printer
(USB-A interface (USB Host)) . . . . . . . . . . . . . . . . . . . . . . . . 103
12.5 Options for data transmission to the USB-B (PC)
interface and the USB-A (USB printer) interface . . . . . . . . . . 104
12.6 Data transmission to the USB-A interface
(USB memory device) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
12.7 MultiLab Importer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

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Contents ProLab 2500

13 Maintenance, cleaning, disposal. . . . . . . . . . . . . . . . . . . . . 106

13.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
13.1.1 General maintenance activities . . . . . . . . . . . . . . . . . . 106
13.1.2 Exchanging the battery. . . . . . . . . . . . . . . . . . . . . . . . . 106
13.2 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
13.3 Packing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
13.4 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

14 What to do if... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

14.1 pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
14.2 ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
14.3 Dissolved oxygen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
14.4 Conductivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
14.5 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

15 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

15.1 Measuring ranges, resolution, accuracy . . . . . . . . . . . . . . . . 115
15.2 General data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

16 Firmware update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119

16.1 Firmware update for the meter ProLab 2500 . . . . . . . . . . . . . 119
16.2 Firmware-Update for IDS Sensors. . . . . . . . . . . . . . . . . . . . . 120

17 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

18 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125

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ProLab 2500 Overview

1 Overview
1.1 Meter ProLab 2500
The ProLab 2500 meter enables you to perform measurements (pH, U, ISE,
conductivity, D.O.) quickly and reliably.
The ProLab 2500 provides the maximum degree of operating comfort, reli-
ability and measuring certainty for all applications.
The ProLab 2500 supports you in your work with the following functions:
 proven calibration procedures
 automatic stability control (AR),
 automatic sensor recognition
 CMC (continuous measurement control)
 QSC (sensor quality control).

1
Pro
Lab
250
0

1 Keypad (antibacterial)
2 Display
3 Connectors

Due to its antibacterial properties, the keypad of the ProLab 2500

is especially suitable for applications in an environment where
hygiene is important (see SECTION 15.2 GENERAL DATA, page 115).

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Overview ProLab 2500

1.2 Sensors

1.2.1 IDS sensors

IDS sensors
 support the automatic sensor recognition
 show only the settings relevant to the specific sensor in the setting menu
 process signals in the sensor digitally so that precise and interference-free
measurements are enabled even with long cables
 facilitate to assign a sensor to a measured parameter with differently colored
couplings
 have quick-lock couplings with which to fix the sensors to the meter.

Sensor data from IDS sensors transmit the following sensor data to the meter:
IDS sensors  SENSOR ID
– Sensor name
– Sensor series number
 Calibration data
 Measurement settings

The calibration data are updated in the IDS sensor after each calibration proce-
dure. A message is displayed while the data are being updated in the sensor.

In the measured value display, you can display the sensor name
and series number of the selected sensor with the [Info] softkey.
You can then display further sensor data stored in the sensor with
the [More] softkey (see section 4.1.6 SENSOR INFO, page 17).

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ProLab 2500 Overview

1.2.2 IDS adapter for analog sensors

With the aid of an IDS adapter, you can also operate analog sensors
on the ProLab 2500. The combination of the IDS adapter and analog sensor
behaves like an IDS sensor.

1.2.3 Automatic sensor recognition

The automatic sensor recognition for IDS sensors allows

 to operate an IDS sensor with different meters without recalibrating
 to operate different IDS sensors at one meter without recalibration
 to assign measurement data to an IDS sensor
– Measurement datasets are always stored and output with the sensor
name and sensor series number.
 to assign calibration data to an IDS sensor
– Calibration data and calibration history are always stored and output with
the sensor name and sensor series number.
 to activate the correct cell constant for conductivity sensors automatically
 to hide menus automatically that do not concern this sensor

To be able to use the automatic sensor recognition, a meter that supports the
automatic sensor recognition (e.g. ProLab 2500) and a digital IDS sensor are
required.
In digital IDS sensors, sensor data are stored that clearly identify the sensor.
The sensor data are automatically taken over by the meter.

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Safety ProLab 2500

2 Safety
2.1 Safety information

2.1.1 Safety information in the operating manual

This operating manual provides important information on the safe operation of

the meter. Read this operating manual thoroughly and make yourself familiar
with the meter before putting it into operation or working with it. The operating
manual must be kept in the vicinity of the meter so you can always find the infor-
mation you need.
Important safety instructions are highlighted in this operating manual. They are
indicated by the warning symbol (triangle) in the left column. The signal word
(e.g. "Caution") indicates the level of danger:

WARNING
indicates a possibly dangerous situation that can lead to seri-
ous (irreversible) injury or death if the safety instruction is not
followed.

CAUTION
indicates a possibly dangerous situation that can lead to slight
(reversible) injury if the safety instruction is not followed.

NOTE
indicates a possibly dangerous situation where goods might be damaged
if the actions mentioned are not taken.

2.1.2 Safety signs on the meter

Note all labels, information signs and safety symbols on the meter. A warning
symbol (triangle) without text refers to safety information in this operating
manual.

2.1.3 Further documents providing safety information

The following documents provide additional information, which you should

observe for your safety when working with the measuring system:
• Operating manuals of sensors and other accessories
• Safety datasheets of calibration or maintenance accessories (such as buffer
solutions, electrolyte solutions, etc.)

2.2 Safe operation

2.2.1 Authorized use

The authorized use of the meter consists exclusively of the measurement of the
pH, ORP, conductivity and dissolved oxygen in a laboratory environment.

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ProLab 2500 Safety

Only the operation and running of the meter according to the instructions and
technical specifications given in this operating manual is authorized (see
section 15 TECHNICAL DATA, page 115).
Any other use is considered unauthorized.

2.2.2 Requirements for safe operation

Note the following points for safe operation:

• The meter may only be operated according to the authorized use specified
above.
• The meter may only be supplied with power by the energy sources
mentioned in this operating manual.
• The meter may only be operated under the environmental conditions
mentioned in this operating manual.
• The meter may not be opened.

2.2.3 Unauthorized use

The meter must not be put into operation if:

• it is visibly damaged (e.g. after being transported)
• it was stored under adverse conditions for a lengthy period of time (storing
conditions, see section 15 TECHNICAL DATA, page 115).

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Commissioning ProLab 2500

3 Commissioning
3.1 Scope of delivery
 MeterProLab 2500
 IDS adapter for analog sensors (Z600 DIN)
 USB cable (A plug on mini B plug)
 Power pack
 Stand with stand base
 Short instructions
 Detailed operating manual
 CD-ROM

3.2 Power supply

The ProLab 2500 is supplied with power in the following ways:
 Mains operation with the supplied power pack
 Operation of the system clock with a buffer battery if there is no mains power
supply (see section 13.1.2 EXCHANGING THE BATTERY, page 106).

3.3 Initial commissioning

Perform the following activities:
 Connect the power pack
(see section 3.3.1 CONNECTING THE POWER PACK, page 13)
 Switch on the meter
(see section 4.2 SWITCHING ON THE METER, page 19)
 Set the date and time
(see section 4.5.5 EXAMPLE 2 ON NAVIGATION: SETTING THE DATE AND TIME,
page 25)
 Mount the stand
(see operating manual of the stand)

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ProLab 2500 Commissioning

3.3.1 Connecting the power pack

CAUTION
The line voltage at the operating site must lie within the input
voltage range of the original power pack (see section 15.2
GENERAL DATA, page 115).

CAUTION
Use original power packs only (see section 15.2 GENERAL DATA,
page 115).

1. Connect the plug of the power pack to the socket for the power pack on
the ProLab 2500.
2. Connect the original power pack to an easily accessible power outlet.
The meter performs a self-test.

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Operation ProLab 2500

4 Operation
4.1 General operating principles

4.1.1 Keypad

In this operating manual, keys are indicated by brackets <..> .

The key symbol (e.g. <ENTER>) generally indicates a short keystroke (press
and release) in this operating manual. A long keystroke (hold for approx. 2 sec)
is indicated by the underscore behind the key symbol (e.g. <ENTER_>).

<F1> Softkeys providing situation dependent functions, e.g.:

<F4> <F1>/[Info]: View information on a sensor

<On/Off> Switches the meter on or off

<M> Selects the measured parameter

<CAL> Calls up the calibration procedure

<CAL_> Displays the calibration data
<AR> Freezes the measured value (HOLD function)
Switches the AutoRead measurement on or off
<ESC> Switches back to the higher menu level /
Cancels inputs
<STO> Saves a measured value manually
<STO_> Opens the menu for the automatic save function
<RCL> Displays the manually stored measured values
<RCL_> Displays the automatically stored measured values

<><> Menu control, navigation

<><>

<ENTER> Opens the menu for measurement settings / Confirms

<ENTER_> entries
Opens the menu for system settings

<PRT> Outputs stored data to the interface

<PRT_> Outputs displayed data to the interface at intervals

14 ba77147e01 07/2016
ProLab 2500 Operation

4.1.2 Display

Example (pH):

4 5
6

2 AutoCal TEC 8
1 HOLD AR
Info 01.06.2016 08:00
9
1 Status information (meter)
2 Status information (sensor)
3 Measured value
4 Measured parameter
5 Continuous measurement control (CMC function)
6 Channel display: Plug position of the sensor
7 Sensor symbol (calibration evaluation, calibration interval)
8 Measured temperature (with unit)
9 Softkeys and date + time

4.1.3 Status information

AutoCal Calibration with automatic buffer recognition, e.g. with the buffer set: Tech-
e.g. TEC nicalbuffers
ConCal Calibration with any buffers
Error An error occurred during calibration
AR Stability control (AutoRead) is active
HOLD Measured value is frozen (<AR> key)
Data are automatically output to the USB-B interface at intervals
Data are output to the USB-A interface (USB flash drive)
Data are output to the USB-A interface (USB printer). If there is a USB-B
connection at the same time (e.g. to a PC), the data are output to the USB-
B interface only.
Connection to a PC is active (USB-B interface)
Data transfer from/to a IDS sensor is active

ba77147e01 07/2016 15
Operation ProLab 2500

4.1.4 Instrument connectors

b a c

6 1 2 3 4 5
1 IDS sensors: (pH, ORP, conductivity, D.O.)
a) Channel 1
b) Channel 2
c) Channel 3
2 USB B (device) interface
3 USB-A (host) interface
4 Power pack
5 Service interface
6 Cover plate
The cover plate closes the mounting slot for the IDS adapter
(Z600 DIN) available as an accessory

CAUTION
Only connect sensors to the meter that cannot return any volt-
ages or currents that are not allowed (> SELV and > current cir-
cuit with current limiting).
Xylem Analytics Germany GmbH IDS sensors and IDS adapt-
ers meet these requirements.

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ProLab 2500 Operation

4.1.5 Channel display

The ProLab 2500 manages the connected sensors and displays which sensor
is plugged to which connection.

Info 01.06.2016 08:00

1 Channel display: Display of the plug position for the respective

parameter
The red bar indicates for each connected sensor to which plug posi-
tion (channel) of the meter it is connected.

4.1.6 Sensor info

You can display the current sensor data and sensor settings of a connected
sensor at any time. The sensor data are available in the measured value dis-
play with the /[Info] softkey.

Info 01.06.2016 08:00

1. In the measured value display:

Display the sensor data (sensor name, series number) with [Info].

ba77147e01 07/2016 17
Operation ProLab 2500

BlueLine 24 pH
IDS
More 01.06.2016 08:00

2. Display further sensor data (settings) with [More].

BlueLine 24 pH IDS
B092500013
Man. temperature: 25 °C
pH resolution 0.001
mV resolution 0.1
Buffer
Calibration interval 7d
Unit for slope mV/pH
QSC: off
Software version 1.00

01.06.2016 08:00

4.1.7 Display of several sensors in the measuring mode

The measured values of the connected sensors can be displayed in the follow-
ing ways:
 Clear display of all connected sensors
 Detailed display of one sensor
(e.g. incl. CMC feature with pH sensors)

With the softkey you can very easily switch between the two display types. The
suitable softkey is displayed depending on the operating situation.

Info 01.06.2016 08:00 Info 01.06.2016 08:00

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ProLab 2500 Operation

4.2 Switching on the meter

1. Switch the meter on with <On/Off>.

The meter performs a self-test.
2. Connect the sensor.
The meter is ready to measure.

Info 01.06.2016 08:00

If the user administration function is activated, the Login dialog

appears after the meter is switched on (see section 4.4 LOGIN WITH
USER NAME, page 19).

The user administration function is not active in the delivery condi-

tion.
The user administration function is activated by the administrator
via the MultiLab User PC software (see the MultiLab User operating
manual).

4.3 Switching off the meter

1. Switch off the meter with <On/Off>.

4.4 Login with user name

After activation of the user administration (see the MultiLab User operating
manual) by the administrator, measurements
are only possible after login with a user name. The user name is documented
with the measured values and in records.
All user names entered by the administrator are listed in the User name menu.
The administrator determines for each user whether or not a password is
required for the login to the meter.
If the Password menu item is grayed out, no password is required for the login.

1. Switch on the meter with <On/Off>.

The Login dialog appears.

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Operation ProLab 2500

Login
User name Admin
Password ####
Change password

01.06.2016 08:00

2. Using <><>, select the menu item, User name and confirm with
<ENTER>.
The user name is highlighted.
3. Using <><>, select a user name and confirm with <ENTER>.

The login is done immediately if no password is required.

If a sensor is connected the measured value display appears.

4. If a password is required:
Using <><>, select the menu item, Password and confirm with
<ENTER>.

The user specifies the password when he or she first logs in with a
user name.
A valid password consists of 4 digits.
The user can change the password with the next login.

5. Change the digit of the highlighted position with <><>.

Switch to the next position of the password with <><>.
When the password was completely entered, confirm with <ENTER>.
The login takes place. If a sensor is connected the measured value dis-
play appears.

Changing the If the administrator has set up the access with password protection:
password
1. Switch on the meter with <On/Off>.
The Login dialog appears.
2. Using <><>, select the menu item, User name and confirm with
<ENTER>.
The user name is highlighted.
3. Using <><>, select a user name and confirm with <ENTER>.
4. Using <><>, select the menu item, Change password and confirm
with <ENTER>.

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ProLab 2500 Operation

5. In the Password field, enter the old password with <><> and
<><>and confirm it with <ENTER>.
6. In the New password field, enter the new password with <><> and
<><>and confirm it with <ENTER>.
The password is changed.
The login takes place. If a sensor is connected the measured value dis-
play appears.

Forgotten the Contact the administrator.

password?

4.5 Navigation

4.5.1 Operating modes

Operating Explanation
mode
Measuring The measurement data of the connected sensor are shown
in the measured value display
Calibration The course of a calibration with calibration information,
functions and settings is displayed
Storage in The meter stores measuring data automatically or manu-
memory ally
Transmitting The meter transmits measuring data and calibration
data records to a USB interface automatically or manually.
Setting The system menu or a sensor menu with submenus, set-
tings and functions is displayed

4.5.2 Measured value display

In the measured value display, you can

 use <><> to select one of several connected sensors. The selected
sensor is displayed with a colored background.
The following actions / menus refer to the selected sensor
 open the menu for calibration and measurement settings with <ENTER>
(short keystroke)
 open the Storage & config menu with the sensor-independent settings by
pressing <ENTER_> (long keystroke, approx. 2 s).
 change the display in the selected measuring screen (e. g. pH <−> mV) by
pressing <M>.

4.5.3 Menus and dialogs

The menus for settings and dialogs in procedures contain further subelements.

ba77147e01 07/2016 21
Operation ProLab 2500

The selection is made with the <><> keys. The current selection is dis-
played with a frame.
 Submenus
The name of the submenu is displayed at the upper edge of the frame. Sub-
menus are opened by confirming with <ENTER>. Example:

System
General
Interface
Clock function
Service information
Reset

01.06.2016 08:00

 Settings
Settings are indicated by a colon. The current setting is displayed on the
right-hand side. The setting mode is opened with <ENTER>. Subsequently,
the setting can be changed with <><> and <ENTER>. Example:

General
Language: English
Audio signal: on
Brightness: 12
Temperature unit: °C
Stability control: on

01.06.2016 08:00

 Functions
Functions are designated by the name of the function. They are immediately
carried out by confirming with <ENTER>. Example: Display the Calibration
record function.

pH
Calibration record
Calibration data storage
Buffer: TEC
Single-point calibration: yes
Calibration interval: 7d
Unit for slope: mV/pH

[i] 2.00 4.0 7.00 10.0

01.06.2016 08:00

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ProLab 2500 Operation

 Messages
Information is marked by the [i] symbol. It cannot be selected. Example:

pH
Calibration record
Calibration data storage
Buffer: TEC
Single-point calibration: yes
Calibration interval: 7d
Unit for slope: mV/pH

[i] 2.00 4.0 7.00 10.0

01.06.2016 08:00

4.5.4 Example 1 on navigation: Setting the language

1. Press the <On/Off> key.

The measured value display appears.
The instrument is in the measuring mode.

Info 01.06.2016 08:00

2. Open the Storage & config menu with <ENTER_>.

The instrument is in the setting mode.

Storage & config

System
Data storage

01.06.2016 08:00

ba77147e01 07/2016 23
Operation ProLab 2500

3. Select the System submenu with <><>.

The current selection is displayed with a frame.
4. Open the System submenu with <ENTER>.

System
General
Interface
Clock function
Service information
Reset

01.06.2016 08:00

5. Select the General submenu with <><>.

The current selection is displayed with a frame.
6. Open the General submenu with <ENTER>.

General
Language: English
Audio signal: on
Brightness: 12
Temperature unit: °C
Stability control: on

01.06.2016 08:00

7. Open the setting mode for the Language with <ENTER>.

General
Language: English
Audio signal: on
Brightness: 12
Temperature unit: °C
Stability control: on

01.06.2016 08:00

8. Select the required language with <><>.

24 ba77147e01 07/2016
ProLab 2500 Operation

9. Confirm the setting with <ENTER>.

The meter switches to the measuring mode.
The selected language is active.

4.5.5 Example 2 on navigation: Setting the date and time

The meter has a clock with a date function. The date and time are indicated in
the status line of the measured value display.
When storing measured values and calibrating, the current date and time are
automatically stored as well.
The correct setting of the date and time and date format is important for the fol-
lowing functions and displays:
 Current date and time
 Calibration date
 Identification of stored measured values.

Therefore, check the time at regular intervals.

The date and time are reset, if the following conditions are met:
 the supply voltage failed and
 the buffer batteries for the system clock are empty.

Setting the date, The date format can be switched from the display of day, month, year
time and date format (dd.mm.yy) to the display of month, day, year (mm/dd/yy or mm.dd.yy).

1. In the measured value display:

Open the Storage & config menu with <ENTER_>.
The instrument is in the setting mode.
2. Select and confirm the System / Clock function menu with <><>
and <ENTER>.
The setting menu for the date and time opens up.
Clock function
Date format: dd.mm.yy
Date: 01.06.2016
Time: 14:53:40

01.06.2016 08:00

3. Select and confirm the Time menu with <><> and <ENTER>.
The hours are highlighted.
4. Change and confirm the setting with <><> and <ENTER>.
The minutes are highlighted.

ba77147e01 07/2016 25
Operation ProLab 2500

5. Change and confirm the setting with <><> and <ENTER>.

The seconds are highlighted.
6. Change and confirm the setting with <><> and <ENTER>.
The time is set.
7. If necessary, set the Date and Date format. The setting is made simi-
larly to that of the time.
8. To make further settings, switch to the next higher menu level with
<ESC>.
or
Switch to the measured value display with <M>.
The instrument is in the measuring mode.

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ProLab 2500 pH value

5 pH value
5.1 Measuring

5.1.1 Measuring the pH value

The sensor connection and the USB-B (device) interface are gal-
vanically isolated. This facilitates interference-free measurements
also in the following cases:
 Measurement in grounded test samples
 Measurement with several sensors connected to one
ProLab 2500 in one test sample

1. Connect the IDS pH sensor to the meter.

The pH measuring window is displayed.
2. If necessary, select the measured parameter with <M>.
3. Adjust the temperature of the solutions and measure the current tem-
perature if the measurement is made without a temperature sensor.
4. If necessary, calibrate or check the IDS pH sensor.
5. Immerse the IDS pH sensor in the test sample.

Info 01.06.2016 08:00

Stability control The stability control function (AutoRead) continually checks the stability of the
(AutoRead) measurement signal. The stability has a considerable impact on the reproduc-
& HOLD function ibility of measured values.
The measured parameter flashes on the display
 as soon as the measured value is outside the stability range
 when the automatic Stability control is switched off.

You can start the Stability control manually at any time, irrespective of the set-
ting for automatic Stability control (see section 10.6.3 AUTOMATIC STABILITY
CONTROL, page 92) in the System menu.

1. Freeze the measured value with <AR>.

The [HOLD] status indicator is displayed.
The HOLD function is active.

ba77147e01 07/2016 27
pH value ProLab 2500

You can terminate the Stability control function and the HOLD func-
tion with <AR> or <M> at any time.

2. Using <ENTER>, activate the Stability control function manually.

The [AR] status indicator appears while the measured value is assessed
as not stable. A progress bar is displayed and the display of the mea-
sured parameter flashes.
As soon as a measured value meets the stability criteria, it is frozen. The
[HOLD][AR] status indicator is displayed, the progress bar disappears
and the display of the measured parameter stops flashing.
The current measurement data is output to the interface. Measurement
data meeting the stability control criterion is marked by AR.

You can prematurely terminate the Stability control function manu-

ally with <ENTER> at any time. When the Stability control function
is prematurely terminated, the current measurement data are out-
put to the interface (PC, USB memory device or USB printer) with-
out AutoRead info.

3. Using <ENTER>, start a further measurement with stability control.

or
Release the frozen measured value again with <AR> or <M>.
The [AR] status display disappears. The display switches back to the pre-
vious indication.

Criteria for a stable The Stability control function checks whether the measured values are stable
measured value within the monitored time interval.

Measured Time interval Stability in the time interval

parameter
pH value 15 seconds ∆ : Better than 0.01 pH

Temperature 15 seconds ∆ : Better than 0.5 °C

The minimum duration until a measured value is assessed as stable is the

monitored time interval. The actual duration is mostly longer.

5.1.2 Measuring the temperature

For reproducible pH measurements, it is essential to measure the temperature

of the test sample.
IDS sensors measure the temperature with a temperature sensor integrated in
the IDS sensor.
When operating a sensor without integrated temperature sensor, e.g.
via an IDS pH adapter, you have to measure and enter the temperature of the
test sample first.

28 ba77147e01 07/2016
ProLab 2500 pH value

The display of the temperature indicates the active temperature measuring

mode:

Temperature Resolution of the Temp. measurement

sensor temp. display
Yes 0.1 °C Automatic with temperature
sensor
- 1 °C Manual

5.2 pH calibration

5.2.1 Why calibrate?

pH electrodes age. This changes the zero point (asymmetry) and slope of the
pH electrode. As a result, an inexact measured value is displayed. Calibration
determines and stores the current values of the zero point and slope of the
electrode.
Thus, you should calibrate at regular intervals.

5.2.2 When do you have to calibrate?

 Routinely within the framework of the company quality assurance

 When the calibration interval has expired

5.2.3 Carrying out automatic calibration (AutoCal)

Make sure that in the sensor menu, Buffer menu, the buffer set is correctly
selected (see section 10.1.1 SETTINGS FOR PH MEASUREMENTS, page 80).
Use one to five buffer solutions of the selected buffer set in any order.
Below, calibration with buffers () is described. When other buffer sets are used,
other nominal buffer values are displayed. Apart from that, the procedure is
identical.

If single-point calibration was set in the menu, the calibration proce-

dure is automatically finished with the measurement of buffer solu-
tion 1 and the calibration record is displayed.

1. Connect the pH sensor to the meter.

The pH measuring window is displayed.
2. Keep the buffer solutions ready.
When measuring without temperature sensor:
Temper the buffer solutions or measure the current temperature.
3. Start the calibration with <CAL>.
The calibration display for the first buffer appears (voltage display).

ba77147e01 07/2016 29
pH value ProLab 2500

01.06.2016 08:00

4. Thoroughly rinse the sensor with deionized water.

5. Immerse the sensor in the first buffer solution.
6. For measurements without temperature sensor
(e.g. when using an IDS adapter):
Enter the temperature of the buffer with <><>.
7. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter
flashes.

10.011

01.06.2016 08:00

8. Wait for the end of the measurement with stability control or accept the
calibration value with <ENTER>.
The calibration display for the next buffer appears (voltage display).
9. If necessary, finish the calibration procedure as a single-point calibration
with <M>.
The calibration record is displayed.

For single-point calibration, the instrument uses the Nernst slope

(-59.2 mV/pH at 25 °C) and determines the zero point of the IDS pH
sensor.

Continuing with two-

10. Thoroughly rinse the sensor with deionized water.
point calibration
11. Immerse the pH sensor in buffer solution 2.

30 ba77147e01 07/2016
ProLab 2500 pH value

12. When measuring without temperature sensor:

Enter the temperature of the buffer with <><>.
13. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter
flashes.

01.06.2016 08:00

14. Wait for the measurement with stability control to be completed or termi-
nate the stability control and take over the calibration value with
<ENTER>.
The calibration display for the next buffer appears (voltage display).
15. If necessary, finish the calibration procedure as a two-point calibration
with <M>.
The calibration record is displayed.

16. Thoroughly rinse the sensor with deionized water.

Continuing with
three- to five-point 17. Immerse the sensor in the next buffer solution.
calibration
18. When measuring without temperature sensor:
Enter the temperature of the buffer with <><>.
19. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter
flashes.

ba77147e01 07/2016 31
pH value ProLab 2500

4.010

01.06.2016 08:00

20. Wait for the measurement with stability control to be completed or termi-
nate the stability control and take over the calibration value with
<ENTER>.
The calibration display for the next buffer appears (voltage display).
21. If necessary, use <M> to finish the calibration.
The calibration record is displayed.
or
Switch to calibration with the next buffer with <ENTER>.

Calibration is automatically completed after the last buffer of a buf-

fer set has been measured. Then the calibration record is dis-
played.
The calibration line is determined by linear regression.

5.2.4 Carrying out a manual calibration (ConCal)

Make sure that in the sensor menu, Buffer menu, the ConCal buffer set is
correctly selected (see section 10.1.1 SETTINGS FOR PH MEASUREMENTS, page
80).
Use any one to five buffer solutions in ascending or descending order.

If single-point calibration was set in the menu, the calibration proce-

dure is automatically finished with the measurement of buffer solu-
tion 1 and the calibration record is displayed.

1. Connect the pH sensor to the meter.

The pH measuring window is displayed.
2. Keep the buffer solutions ready.
When measuring without temperature sensor:
Temper the buffer solutions or measure the current temperature.
3. Start the calibration with <CAL>.
The calibration display for the first buffer appears (voltage display).

32 ba77147e01 07/2016
ProLab 2500 pH value

01.06.2016 08:00

4. Thoroughly rinse the sensor with deionized water.

5. Immerse the pH sensor in buffer solution 1.
6. For measurements without temperature sensor
(e.g. when using an IDS adapter):
Enter the temperature of the buffer with <><>.
7. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter flashes.

01.06.2016 08:00

8. Wait for the measurement with stability control to be completed or ter-

minate the stability control and take over the calibration value with
<ENTER>.
The pH value of the buffer solution is displayed.

01.06.2016 08:00

ba77147e01 07/2016 33
pH value ProLab 2500

9. Set the nominal buffer value for the measured temperature with
<><>.
10. Accept the calibration value with <ENTER>.
The calibration display for the next buffer appears (voltage display).
11. If necessary, finish the calibration procedure as a single-point calibration
with <M>.
The calibration record is displayed.

For single-point calibration, the instrument uses the Nernst slope

(-59.2 mV/pH at 25 °C) and determines the zero point of the IDS pH
sensor.

Continuing with two-

12. Thoroughly rinse the sensor with deionized water.
point calibration
13. Immerse the pH sensor in buffer solution 2.
14. When measuring without temperature sensor:
Enter the temperature of the buffer with <><>.
15. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter
flashes.
16. Wait for the measurement with stability control to be completed or ter-
minate the stability control and take over the calibration value with
<ENTER>.
The pH value of the buffer solution is displayed.

01.06.2016 08:00

17. Set the nominal buffer value for the measured temperature with
<><>.
18. Accept the calibration value with <ENTER>.
The calibration display for the next buffer appears (voltage display).
19. If necessary, finish the calibration procedure as a two-point calibration
with <M>.
The calibration record is displayed.

34 ba77147e01 07/2016
ProLab 2500 pH value

Continuing with
20. Thoroughly rinse the sensor with deionized water.
three- to five-point
calibration 21. Immerse the sensor in the next buffer solution.
22. When measuring without temperature sensor:
Enter the temperature of the buffer with <><>.
23. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter
flashes.
24. Wait for the measurement with stability control to be completed or ter-
minate the stability control and take over the calibration value with
<ENTER>.
The pH value of the buffer solution is displayed.

01.06.2016 08:00

25. Set the nominal buffer value for the measured temperature with
<><>.
26. Accept the calibration value with <ENTER>.
The calibration display for the next buffer appears (voltage display).
27. If necessary, use <M> to finish the calibration.
The calibration record is displayed.
or
Continue calibrating using the next buffer with <ENTER>.

After the fifth buffer has been measured the calibration is automat-
ically finished. Then the calibration record is displayed.
The calibration line is determined by linear regression.

5.2.5 Calibration points

Calibration can be performed using one to five buffer solutions in any order
(single-point to five-point calibration). The meter determines the following
values and calculates the calibration line as follows:

ba77147e01 07/2016 35
pH value ProLab 2500

Calibration Determined val- Displayed calibration data

ues
1-point Asy  Zero point = Asy
 Slope = Nernst slope
(-59.2 mV/pH at 25 °C)
2-point Asy  Zero point = Asy
Slp.  Slope = Slp.
3-point to 5- Asy  Zero point = Asy
point Slp.  Slope = Slp.
The calibration line is calculated by
linear regression.

You can display the slope in the units, mV/pH or % (see section
10.1.1 SETTINGS FOR PH MEASUREMENTS, page 80).

5.2.6 Calibration data

The calibration record is automatically transmitted to the interface

after calibrating.

Displaying the cali- The calibration record of the last calibration is to be found under the menu item,
bration data Calibration / Calibration record. To open it in the measured value display, press
the <CAL_> key.
The calibration records of the last 10 calibrations are to be found in the menu,
Calibration / Calibration data storage / Display. To open the Calibration menu,
press the <ENTER> key in the measured value display.

36 ba77147e01 07/2016
ProLab 2500 pH value

Menu item Setting/ Explanation

function
Calibration / - Displays the calibration records.
Calibration data stor- Further options:
age /Display  Scroll through the calibration
records with <><>.
 Using <PRT>, output the dis-
played calibration record to the
USB-B (PC) interface or the USB-
A (USB printer) interface.
 Using <PRT_>, output all calibra-
tion records to the USB-B (PC)
interface or the USB-A (USB
printer) interface.
 Quit the display with <ESC>.
 Switch directly to the measured
value display with <M>.
Calibration / Calibra- - Outputs the stored calibration data to
tion data storage / the USB-A interface (USB memory
Output to USB flash device/USB printer)
drive or printer
Calibration / - Outputs the stored calibration data to
Calibration data stor- the USB-B interface (PC)
age /
Output to RS232/USB

Calibration evalua- After calibrating, the meter automatically evaluates the calibration. The zero
tion point and slope are evaluated separately. The worse evaluation of both is taken
into account. The evaluation appears on the display and in the calibration
record.

Display Calibration Zero point Slope [mV/pH]

record [mV]
+++ -15 ... +15 -60.5 ... -58.0

++ -20 ... <-15 >-58.0 ... -57.0

or
>+15 ... +20

+ -25 ... <-20 -61.0 ... <-60.5

or or
>+20 ... +25 >-57.0 ... -56.0

- -30 ... <-25 -62.0 ... <-61.0

or or
->+25 ... +30 >-56.0 ... -50.0

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pH value ProLab 2500

Display Calibration Zero point Slope [mV/pH]

record [mV]
Clean the IDS sensor according
to the sensor operating manual
Error Error <-30 <-62.0
or or
>+30 > -50,0

Error elimination (see section 14

WHAT TO DO IF..., page 109)

For pH IDS sensors you can optionally enable a more finely graded
calibration evaluation (QSC) (see section 5.2.8 QSC FUNCTION
(SENSOR QUALITY CONTROL), page 40).

Calibration record
CALIBRATION pH
01.06.2016 07:43:33
BlueLine 24 pH IDS
Ser. no. B092500013

Buffer 1 4.01
Buffer 2 7.00
Buffer 3 10.01
Voltage 1 184.0 mV
Voltage 2 3.0 mV
Voltage 3 -177.0 mV
Temperature 1 24.0 °C
Temperature 2 24.0 °C
Temperature 3 24.0 °C
Slope -60.2 mV/pH
Asymmetry 4.0 mV
Sensor +++

etc...

5.2.7 Continuous measurement control (CMC function)

The Continuous Measurement Control (CMC function) facilitates to evaluate

the current measured value instantly and definitely.
After each successful calibration the scale of the pH measuring range is
displayed in the measured value display. Here you can very clearly see
whether or not the current measured value is in the calibrated part of the
measuring range.

38 ba77147e01 07/2016
ProLab 2500 pH value

The following information is displayed:

1
2

3
4

Info 01.06.2016 08:00

1 Currently measured pH value (needle)

2 Marking lines for all nominal buffer values used with the last valid cali-
bration
3 Measuring range for which a valid calibration is available. Measured
values in this range are suitable for documentation.
4 Measuring range for which no valid calibration is available (dark
gray). Measured values in this range are not suitable for documenta-
tion. Calibrate the meter with buffers covering this measuring range.
If the current measured value is outside the calibrated range, this area
is displayed in a darker gray.
If a measured value is outside the measuring range pH 0 - 14, over-
flow arrows are displayed at the left or right edge of the measuring
range.

The limits of the calibrated range are determined by the buffers used for cali-
bration:

Lower limit: Buffer with lowest pH value - 2 pH units

Upper limit: Buffer with highest pH value + 2 pH units

ba77147e01 07/2016 39
pH value ProLab 2500

5.2.8 QSC function (sensor quality control)

General information The QSC function (Quality Sensor Control) is a new sensor evaluation for
on the QSC function digital IDS sensors. It evaluates the condition of an IDS pH sensor individually
and with a very fine grading.
The QSC scale shows the current sensor evaluation with an indicator on the
display.

Info 01.06.2016 08:00

1 QSC scale
The double arrow on the QSC scale indicates the current sensor eval-
uation

In the USB output the sensor evaluation is given as a percentage (1-100).

The finely graded sensor evaluation of the QSC function promptly calls your
attention to changes of the sensor.
Thus you can do what is necessary to restore the optimum measuring quality
(e.g. clean, calibrate or replace the sensor).

Sensor evaluation With QSC function Without QSC function (sensor sym-
with / without bol)
QSC function Very fine grading of the sensor eval- Rough grading of the sensor evalua-
uation (100 grades) tion (4 grades)
The reference value is individually A theoretical reference value is used
determined for each sensor during for all sensors
the QSC initial calibration.
Low tolerances for zero point and Greater tolerances for zero point and
slope when using QSC buffer solu- slope when using commercial buffer
tions sets
Additional QSC calibration required No additional calibration required
(with special QSC buffer set)

QSC calibration The QSC function is enabled by once carrying out an additional three-point cali-
bration with special QSC buffer solutions. It covers the measuring range of the
sensor (pH 2 to pH 11). The QSC initial calibration determines the actual condi-
tion of the sensor and stores it as a reference in the sensor.
To meet the high requirements of a QSC initial calibration, the QSC initial cali-
bration should optimally be carried out with the initial commissioning of the
sensor.

40 ba77147e01 07/2016
ProLab 2500 pH value

Carry out the normal calibrations for your special measuring range with your
usual standard solutions as previously done.

As soon as the QSC function was enabled for an IDS sensor, it is

not possible to return to the sensor evaluation with the sensor
symbol for this sensor.

Carrying out a QSC

1. Open the menu for measurement settings with <ENTER>.
initial calibration
2. In the QSC menu, select First calibration with <><>.
The calibration display appears. AutoCal QSC-Kit is displayed as the
buffer.
Exclusively use the QSC-Kit for the QSC calibration. If you use other
buffers, you will have no valid QSC calibration.

01.06.2016 08:00

3. Calibration with the buffers of the QSC-Kit is done like a normal three-
point calibration.
Follow the user guide.

Carry out the QSC initial calibration very carefully. It determines the
reference value for the sensor. This reference value cannot be
overwritten or reset.
As soon as the QSC function was enabled, it is not possible to
return to the sensor evaluation with the sensor symbol.

4. As soon as the three-point calibration has been successfully carried out

you can decide whether to accept or discard the calibration as the QSC
initial calibration.

The QSC initial calibration is completed. The sensor is calibrated. If you want
to calibrate with special buffers for your measurements, you can subsequently
carry out a normal calibration with your buffers. The reference values deter-
mined with the QSC calibration are also used for the evaluation of normal cali-
brations. In the measured value display, the QSC scale of the QSC function is
always displayed. A double arrow on the QSC scale indicates the current sen-

ba77147e01 07/2016 41
pH value ProLab 2500

sor evaluation.

Info 01.06.2016 08:00

1 QSC scale
The double arrow on the QSC scale indicates the current sensor eval-
uation

Carrying out a QSC A QSC control calibration can, e.g. be useful if the sensor evaluation noticeably
control calibration changed (after some normal calibrations).
You can carry out QSC control calibrations at greater intervals than normal cali-
brations.

1. Open the menu for measurement settings with <ENTER>.

2. In the QSC menu, select Control calibration with <><>.
The calibration display appears. AutoCal QSC-Kit is displayed as the buf-
fer.
Exclusively use the QSC-Kit for the QSC calibration. If you use other buf-
fers, you will have no valid QSC control calibration.
3. Follow the user guide.
The calibration is carried out like a normal three-point calibration. As soon
as the three-point calibration has been successfully carried out you can
decide whether to accept or discard the calibration as the QSC control
calibration.

42 ba77147e01 07/2016
ProLab 2500 ORP voltage

6 ORP voltage
6.1 Measuring

6.1.1 Measuring the ORP

The sensor connection and the USB-B (device) interface are gal-
vanically isolated. This facilitates interference-free measurements
also in the following cases:
 Measurement in grounded test samples
 Measurement with several sensors connected to one
ProLab 2500 in one test sample

IDS ORP sensors are not calibrated. However, you can check IDS
ORP sensors using a test solution.

1. Connect the ORP sensor to the meter.

The ORP measuring window is displayed.
2. Adjust the temperature of the solutions and measure the current tem-
perature if the measurement is made without a temperature sensor.
3. Check the meter with the ORP sensor.
4. Immerse the ORP sensor in the test sample.

Info 01.06.2016 08:00

Stability control The stability control function (AutoRead) continually checks the stability of the
(AutoRead) measurement signal. The stability has a considerable impact on the reproduc-
& HOLD function ibility of measured values.
The measured parameter flashes on the display
 as soon as the measured value is outside the stability range
 when the automatic Stability control is switched off.

You can start the Stability control manually at any time, irrespective of the
setting for automatic Stability control (see section 10.6.3 AUTOMATIC STABILITY
CONTROL, page 92) in the System menu.

ba77147e01 07/2016 43
ORP voltage ProLab 2500

1. Freeze the measured value with <AR>.

The [HOLD] status indicator is displayed.
The HOLD function is active.

You can terminate the Stability control function and the HOLD func-
tion with <AR> or <M> at any time.

2. Using <ENTER>, activate the Stability control function manually.

The [AR] status indicator appears while the measured value is assessed
as not stable. A progress bar is displayed and the display of the mea-
sured parameter flashes.
As soon as a measured value meets the stability criteria, it is frozen. The
[HOLD][AR] status indicator is displayed, the progress bar disappears
and the display of the measured parameter stops flashing.
The current measurement data is output to the interface. Measurement
data meeting the stability control criterion is marked by AR.

You can prematurely terminate the Stability control function manu-

ally with <ENTER> at any time. When the Stability control function
is prematurely terminated, the current measurement data are out-
put to the interface (PC, USB memory device or USB printer) with-
out AutoRead info.

3. Using <ENTER>, start a further measurement with stability control.

or
Release the frozen measured value again with <AR> or <M>.
The [AR] status display disappears. The display switches back to the pre-
vious indication.

Criteria for a stable The Stability control function checks whether the measured values are stable
measured value within the monitored time interval.

Measured parame- Time interval Stability in the time interval

ter
ORP 15 seconds ∆ : Better than 0.3 mV

Temperature 15 seconds ∆ : Better than 0.5 °C

The minimum duration until a measured value is assessed as stable is the

monitored time interval. The actual duration is mostly longer.

6.1.2 Measuring the relative ORP

To measure the difference of the ORPs of two solutions, you have to define the
ORP of one solution as the zero point first.

44 ba77147e01 07/2016
ProLab 2500 ORP voltage

ORP electrodes can be used to determine the relative ORP.

1. Connect the ORP electrode to the meter.

2. Prepare the reference solution for the determination of the reference
point.
3. Select the ΔU (mV display with <M>.
4. Immerse the ORP electrode in the reference solution.
5. Display the potential of the current zero point with <CAL>.
6. Press <ENTER> to measure the reference solution.
The measured value is checked for stability (automatic stability con-
trol). The display of the measured parameter flashes.
The measured potential is defined as the zero point.
or
Press <M> to terminate the display of the zero point.
7. Rinse the ORP electrode and immerse it in the test sample.
The measured value is checked for stability (automatic stability con-
trol). The display of the measured parameter flashes.
8. Wait for a stable measured value.
The display of the measured parameter no longer flashes.

Info 01.06.2016 08:00

AutoRead The Stability control function checks whether the measured values are stable
within the monitored time interval.

Measured parame- Time interval Stability in the time interval

ter
ORP 15 seconds ∆ : better than 0.3 mV

Temperature 15 seconds ∆ : better than 0.5 °C

The minimum duration until a measured value is assessed as stable is the

monitored time interval. The actual duration is mostly longer.

ba77147e01 07/2016 45
ORP voltage ProLab 2500

6.1.3 Measuring the temperature

For reproducible ORP measurements, it is essential to measure the tempera-

ture of the test sample.
When operating a sensor without integrated temperature sensor, you first have
to measure and enter the temperature of the sample.
The measuring instrument recognizes whether a suitable sensor is connected
and automatically switches on the temperature measurement.
The display of the temperature indicates the active temperature measuring
mode:

Temperature Resolution of Temp. measurement

sensor the temp. dis-
play
Yes 0.1 °C Automatic with temperature sensor
- 1 °C Manual

6.2 ORP calibration

ORP electrodes are not calibrated. You can, however, check ORP
electrodes by measuring the ORP of a test solution and comparing
the value with the nominal value.

46 ba77147e01 07/2016
ProLab 2500 Ion concentration

7 Ion concentration
7.1 Measuring

7.1.1 Measuring the ion concentration

The sensor connection and the USB-B (device) interface are gal-
vanically isolated. This facilitates interference-free measurements
also in the following cases:
 Measurement in grounded test samples
 Measurement with several sensors connected to one
ProLab 2500 in one test sample

Incorrect calibration of ion selective electrodes will result in incor-

rect measured values. Calibrate regularly before measuring.

For precise ISE measurements the temperature difference

between measurement and calibration should not be greater that
2 K. Therefore, adjust the temperature of the standard and
measuring solutions accordingly. If the temperature difference is
greater the [TpErr] warning appears in the measured value display.

1. Connect the ISE combination electrode to the meter with the aid of an
ADA 94/IDS adapter.
The pH/ISE measuring window is displayed.
2. In the measured value display, select the ISE measuring window with
<> <> and <M>.
3. If necessary, change the ion type in the ISE setup/Ion type menu.
4. If necessary, measure the temperature of the test sample with a ther-
mometer.
5. Calibrate or check the meter with the electrode.

While no valid calibration is available, e.g. in the delivery condition,

"Error" appears in the measured value display.

6. Immerse the electrode in the test sample.

ba77147e01 07/2016 47
Ion concentration ProLab 2500

01.06.2016 08:00

Stability control The stability control function (AutoRead) continually checks the stability of the
(AutoRead) measurement signal. The stability has a considerable impact on the reproduc-
& HOLD function ibility of measured values.
The measured parameter flashes on the display
 as soon as the measured value is outside the stability range
 when the automatic Stability control is switched off.

You can activate or switch off the automatic Stability control function (see
section 10.6.3 AUTOMATIC STABILITY CONTROL, page 92).

1. Freeze the measured value with <AR>.

The [HOLD] status indicator is displayed.
The HOLD function is active.

You can terminate the Stability control function and the HOLD func-
tion with <AR> or <M> at any time.

2. Using <ENTER>, activate the Stability control function manually.

The [AR] status indicator appears while the measured value is
assessed as not stable. A progress bar is displayed and the display of
the measured parameter flashes.
The [HOLD][AR] status indicator appears as soon as a stable mea-
sured value is recognized. The progress bar disappears and the dis-
play of the measured parameter stops flashing.
The current measurement data is output to the interface. Measurement
data meeting the stability control criterion is marked by AR.

You can prematurely terminate the Stability control function manu-

ally with <ENTER> at any time. If the Stability control function is
prematurely terminated, the current measurement data are output
to the interface without the AutoRead info.

48 ba77147e01 07/2016
ProLab 2500 Ion concentration

3. Using <ENTER>, start a further measurement with stability control.

or
Release the frozen measured value again with <AR> or <M>.
The [AR] status display disappears. The display switches back to the
previous indication.

Criteria The AutoRead criteria affect the reproducibility of the measured values. The
following criteria can be adjusted:
 high: highest reproducibility
 medium: medium reproducibility
 low: lowest reproducibility

Increasing reproducibility also causes the response time to

increase until a measured value is evaluated as stable.

7.1.2 Measuring the temperature

For reproducible ion-selective measurements, it is essential to measure the

temperature of the test sample.
You have the following options to measure the temperature:
 Measurement by an external temperature sensor.
 Manual determination and input of the temperature.

The measuring instrument recognizes whether a suitable sensor is connected

and automatically switches on the temperature measurement.
The display of the temperature indicates the active temperature measuring
mode:

Temperatur Resolution of the

e sensor temp. display Temp. measurement

yes 0.1 °C Automatic with temperature sensor

- 1 °C Manual

If you wish to measure (or calibrate) without temperature sensor, proceed as

follows:

1. Measure the current temperature of the test sample.

2. Set the temperature value with <><>.
or
In the <ENTER>/ISE/Man. temperature menu, set the temperature value
with <><>.

ba77147e01 07/2016 49
Ion concentration ProLab 2500

7.2 Calibration

7.2.1 Why calibrate?

Ion-selective electrodes age and are temperature-dependent. This changes

the slope. As a result, an inexact measured value is displayed. Calibration
determines the calibration line of the electrode and stores this value in the
meter.
Thus, you should calibrate before each measurement and at regular intervals.

7.2.2 When to calibrate?

 Before any measurement if possible

 After connecting another ISE electrode

7.2.3 Calibration (ISE Cal)

ISE Cal is the conventional two-point to seven-point calibration procedure

that uses 2 to 7 freely selectable standard solutions. The concentration
expected for the measurement determines the concentration of the calibration
standards.

1. Connect the ISE combination electrode to the meter.

The pH/mV/ISE measuring window is displayed.
2. Keep the standard solutions ready.
3. If necessary, measure the temperature of the standard solutions with a
thermometer.
4. In the measured value display, select the ISE measuring window with
<> <> and <M>.
5. If necessary, change the ion type in the ISE setup/Ion type menu.
6. If necessary, change the unit of the measurement result and calibration
standards in the ISE setup/Unit menu.
7. Start the calibration with <CAL>.
The calibration display appears.

Standard

01.06.2016 08:00

8. Thoroughly rinse the electrode with distilled water.

50 ba77147e01 07/2016
ProLab 2500 Ion concentration

9. Immerse the electrode in standard solution 1.

10. When calibrating without temperature sensor:
 Measure the temperature of the standard solution using a thermom-
eter.
 Use <F4>/[ ⇅ ] to select the setting of the temperature.
 Use <> <> to set the temperature.
 Use <F4>/[ ⇅ ] to select the setting of the concentration.
11. Set the concentration of the standard solution with <> <> and
press <ENTER>.
The standard solution is measured.
The measured value is checked for stability (AutoRead).

01.06.2016 08:00

12. Wait for the end of the AutoRead measurement or accept the calibra-
tion value with <ENTER>.
The calibration display for the next standard solution appears.

Standard

01.06.2016 08:00

Continuing with two-

13. Thoroughly rinse the electrode with distilled water.
point calibration
14. Immerse the electrode in standard solution 2.

ba77147e01 07/2016 51
Ion concentration ProLab 2500

15. When calibrating without temperature sensor:

 Measure the temperature of the standard solution using a thermom-
eter.
 Use <F4>/[ ⇅ ] to select the setting of the temperature.
 Use <> <> to set the temperature.
 Use <F4>/[ ⇅ ] to select the setting of the concentration.
16. Set the concentration of the standard solution with <> <> and
press <ENTER>.
The standard solution is measured.
The measured value is checked for stability (AutoRead).

01.06.2016 08:00

17. Wait for the end of the AutoRead measurement or accept the calibra-
tion value with <ENTER>.
The calibration display for the next standard solution appears.

Standard

01.06.2016 08:00

18. Press <ENTER> to continue with three-point calibration.

or
Finish the calibration procedure as a two-point calibration with <M>.
The new calibration values are displayed.

Continuing with Repeat the steps 8 to 13 in the same way with the third and further standard
three- to seven-point solutions as necessary. The new calibration values are displayed after the last
calibration calibration step was completed.

Based on the calibration data, the calibration curve is determined

in sections, according to the Nernst equation modified by Nikolski.

52 ba77147e01 07/2016
ProLab 2500 Ion concentration

7.2.4 Calibration standards

Use two to seven different standard solutions. The standard solutions have to
be selected in either increasing or decreasing order.
Select the unit of the standard solution and measurement result in
the ISE setup/Unit menu.

Standard solution (Std 1 - 7) Values

Unit [mg/l] 0.010 ... 500,000
Unit [mol/l] 0.100 ... 5,000 µmol/l
10.00 ... 5,000 mmol/l
Unit [mg/kg] 0.010 ... 500,000
Unit [ppm] 0.010 ... 500,000
Unit [%] 0.001 ... 50,000

The measurement precision is also dependent on the selected

standard solutions. Therefore, the selected standard solutions
should cover the value range expected of the subsequent concen-
tration measurement.
If the measured electrode potential is outside the calibrated range,
the [ISEErr] warning is displayed.

7.2.5 Calibration data

The calibration record is automatically transmitted to the interface

after calibrating.

Displaying the cali- The calibration record of the last calibration is to be found under the menu item,
bration data Calibration / Calibration record. To open it in the measured value display, press
the <CAL_> key.
The calibration records of the last 10 calibrations are to be found in the menu,
Calibration / Calibration data storage / Display. To open the Calibration menu,
press the <ENTER> key in the measured value display.

ba77147e01 07/2016 53
Ion concentration ProLab 2500

Menu item Setting/ Explanation

function
Calibration / - Displays the calibration records.
Calibration data stor- Further options:
age /Display  Scroll through the calibration
records with <><>.
 Using <PRT>, output the dis-
played calibration record to the
USB-B (PC) interface or the USB-
A (USB printer) interface.
 Using <PRT_>, output all calibra-
tion records to the USB-B (PC)
interface or the USB-A (USB
printer) interface.
 Quit the display with <ESC>.
 Switch directly to the measured
value display with <M>.
Calibration / Calibra- - Outputs the stored calibration data to
tion data storage / the USB-A interface (USB memory
Output to USB flash device/USB printer)
drive or printer
Calibration / - Outputs the stored calibration data to
Calibration data stor- the USB-B interface (PC)
age /
Output to RS232/USB

Calibration After calibrating, the meter automatically evaluates the calibration.

evaluation
Display Calibration record Magnitude of the slope [mV]
+++ 30.0 ... 90.0 *
or 15.0 ... 45.0 **

Error Error < 30 or > 90.0 *

or < 15 or > 45 **
Error elimination (see section 14.2 * monovalent ions
ISE, page 111) ** divalent ions

54 ba77147e01 07/2016
ProLab 2500 Ion concentration

Calibration record ProLab 2500

(example) Ser. no. 12345678

CALIBRATION ISE
01.06.2016 08:09:10

Sensor
Ser. no. 12345678

Standard 1 0.010 mg/l

Standard 2 0.020 mg/l
Voltage 1 38.5 mV
Voltage 2 58.0 mV
Temperature 1 24.0 øC
Temperature 2 24.0 øC
Ion type Ag
Slope 54.1 mV
Sensor +++
_____________________________________

ba77147e01 07/2016 55
Ion concentration ProLab 2500

7.3 Selecting the measuring method

The following methods are supported:
 Standard addition
 Standard subtraction
 Sample addition
 Sample subtraction
 Blank value addition

1. Connect the ISE combination electrode to the meter.

The pH/ISE measuring window is displayed.
2. If necessary, select the ISE display with <M>.
3. If necessary, measure the temperature of the test sample with a ther-
mometer.
4. Open the ISE menu with <ENTER>.
5. Thoroughly rinse the electrode with distilled water.
6. Adjust the temperature of the standard solutions.
7. Select Method with <> <> and confirm with <ENTER>.
8. Select a method with <> <> and confirm with <ENTER>.
ISE
Calibration
Man. temperature: 25 °C
ISE setup
Method: Standard addition
Start method

01.06.2016 08:00

9. Select Start method with <> <> and confirm with <ENTER>.
Measurement with the selected method begins (see section 7.3.1
STANDARD ADDITION, page 57 ... section 7.3.5 STANDARD ADDITION WITH
BLANK VALUE CORRECTION (BLANK VALUE ADDITION), page 66).

56 ba77147e01 07/2016
ProLab 2500 Ion concentration

7.3.1 Standard addition

In the "Standard addition" procedure, a known amount of standard solution is

added to the sample.
The ion concentration in the sample is calculated from the change in potential.

1. Select the measuring method (see section 7.3 SELECTING THE MEASU-
RING METHOD, page 56).

2. Prepare two standard calibration solutions.

3. Perform a two-point calibration according to user guidance.
4. The calibration record is displayed as soon as a stable value is
achieved for the second standard calibration solution.
CALIBRATION
Z600
B092500013

01.06.2016 08:00

#1 0.010 mg/l 20.2 mV 25.0 °C

#2 0.020 mg/l 79.2 mV 25.0 °C
Slp.: 54.1 mV
Sensor +++ (Ion type: Ag)

01.06.2016 08:00

Measuring
5. Start the measurement with <F1>/[continue].
An entry window appears.
Standard addition

i Immerse sensor in sample

Sample volume 100.0 ml

ISA/TISAB vol. 1.0 ml
continue

01.06.2016 08:00

6. Prepare the sample.

7. If necessary, add the ISA/TISAB solution to the sample.
8. Thoroughly rinse the electrode with deionized water.
9. Immerse the electrode in the sample.
10. Using <> <> and <ENTER>, select the values for the volume of
the sample (Sample volume) and the volume of the ISA/TISAB solution
(ISA/TISAB vol.).

ba77147e01 07/2016 57
Ion concentration ProLab 2500

11. Select continue with <> <> and start the measurement with
<ENTER>.
When the measurement is finished an entry window appears.

Standard addition

i Add standard!

Std. volume 1.0 ml

Std. conc. 1.0 mg/l
continue

01.06.2016 08:00

12. Add the standard solution to the sample.

13. Using <> <> and <ENTER>, enter the values for the volume of the
standard solution (Std. volume) and concentration of the standard solu-
tion (Std. conc.) .
14. Select continue with <> <> and start the measurement with
<ENTER>.
The measurement result is displayed when the measurement is com-
pleted.
Standard addition

01.06.2016 08:00

15. If necessary, start measuring further samples with <ENTER>.

Repeat steps 6 - 14 for all samples.
16. Terminate the measuring method with <M>.
A safety query appears.
17. Select yes with <> <>.
18. Confirm yes with <ENTER>.
Measurement with the selected method is completed.

58 ba77147e01 07/2016
ProLab 2500 Ion concentration

7.3.2 Standard subtraction

In the "Standard Subtraction" procedure, a known amount of standard solution

is added to the sample (as complexing agent or precipitating agent) and, thus,
the ion concentration lowered.
The ion concentration in the sample is calculated from the change in potential.

1. Select the measuring method (see section 7.3 SELECTING THE MEASU-
RING METHOD, page 56).

Calibration
2. Prepare two standard calibration solutions.
3. Perform a two-point calibration according to user guidance.
4. The calibration record is displayed as soon as a stable value is
achieved for the second standard calibration solution.

CALIBRATION
Z600
B092500013

01.06.2016 08:00

#1 0.010 mg/l 20.2 mV 25.0 °C

#2 0.020 mg/l 79.2 mV 25.0 °C
Slp.: 54.1 mV
Sensor +++ (Ion type: Ag)

01.06.2016 08:00

Measuring
5. Start the measurement with <F1>/[continue].
An entry window appears.

Standard subtraction

i Immerse sensor in sample

Sample volume 100.0 ml

ISA/TISAB vol. 1.0 ml
continue

01.06.2016 08:00

6. Prepare the sample.

7. If necessary, add the ISA/TISAB solution to the sample.
8. Thoroughly rinse the electrode with deionized water.

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Ion concentration ProLab 2500

9. Immerse the electrode in the sample.

10. Using <> <> and <ENTER>, select the values for the volume of
the sample (Sample volume) and the volume of the ISA/TISAB solution
(ISA/TISAB vol.).
11. Select continue with <> <> and start the measurement with
<ENTER>.
When the measurement is finished an entry window appears.

Standard subtraction

i Add standard!

Ion type S
Std. volume 1.0 ml
Std. conc. 1.0 mg/l
continue

01.06.2016 08:00

12. Add the standard (complexing agent or precipitating agent) to the sam-
ple.
13. Using <> <> and <ENTER>, enter the values for the volume of the
standard solution (Std. volume) and concentration of the standard solu-
tion (Std. conc.) .
14. If necessary, set the ion type for the ion in the standard (Ion type) with
<> <> and <ENTER>.
On selection of the ion type that can be defined (ION):
Using <> <> and <ENTER>, set the valence (Valency) and molar
weight (Molar mass) for the ion in the standard solution.
15. Select continue with <> <> and start the measurement with
<ENTER>.
The measurement result is displayed when the measurement is com-
pleted.
Standard subtraction

01.06.2016 08:00

16. If necessary, start measuring further samples with <ENTER>.

Repeat steps 6 - 13 for all samples.

60 ba77147e01 07/2016
ProLab 2500 Ion concentration

17. Terminate the measuring method with <M>.

A safety query appears.
18. Select yes with <> <>.
19. Confirm yes with <ENTER>.
Measurement with the selected method is completed.

ba77147e01 07/2016 61
Ion concentration ProLab 2500

7.3.3 Sample addition

In the "Sample addition" procedure, a known amount of sample is added to the

standard solution.
The ion concentration in the sample is calculated from the change in potential.

1. Select the measuring method (see section 7.3 SELECTING THE MEASU-
RING METHOD, page 56).

Calibration
2. Prepare two standard calibration solutions.
3. Perform a two-point calibration according to user guidance.
4. The calibration record is displayed as soon as a stable value is
achieved for the second standard calibration solution.
CALIBRATION
Z600
B092500013

01.06.2016 08:00

#1 0.010 mg/l 20.2 mV 25.0 °C

#2 0.020 mg/l 79.2 mV 25.0 °C
Slp.: 54.1 mV
Sensor +++ (Ion type: Ag)

01.06.2016 08:00

Measuring
5. Start the measurement with <F1>/[continue].
An entry window appears.
Sample addition

i Immerse sensor in standard

Std. volume 100.0 ml

Std. conc. 1.0 mg/l
ISA/TISAB vol. 1.0 ml
continue

01.06.2016 08:00

6. Prepare the standard solution.

7. If necessary, add the ISA/TISAB solution to the standard solution.
8. Thoroughly rinse the electrode with deionized water.
9. Immerse the electrode in the standard.

62 ba77147e01 07/2016
ProLab 2500 Ion concentration

10. Using <> <> and <ENTER>, enter the values for the volume of the
standard solution (Std. volume), the concentration of the standard solu-
tion (Std. conc.) and the volume of the ISA/TISAB solution (ISA/TISAB
vol.).
11. Select continue with <> <> and start the measurement with
<ENTER>.
When the measurement is finished an entry window appears.

Sample addition

i Add sample!

Sample volume 1.0 ml

continue

01.06.2016 08:00

12. Add the sample to the standard solution.

13. Using <> <> and <ENTER>, enter the value for the volume of the
sample (Sample volume).
14. Select continue with <> <> and start the measurement with
<ENTER>.
The measurement result is displayed when the measurement is com-
pleted.
Sample addition

01.06.2016 08:00

15. If necessary, start measuring further samples with <ENTER>.

Repeat steps 6 - 12 for all samples.
16. Terminate the measuring method with <M>.
A safety query appears.
17. Select yes with <> <>.
18. Confirm yes with <ENTER>.
Measurement with the selected method is completed.

ba77147e01 07/2016 63
Ion concentration ProLab 2500

7.3.4 Sample subtraction

With the "Sample subtraction" procedure, a known amount of sample is added

to the standard solution (complexing agent or precipitating agent).
The ion concentration in the sample is calculated from the change in potential.
The sample subtraction is one method for the indirect determination of ions. It
is used for the determination of ions for which no direct determination is possi-
ble.

1. Select the measuring method (see section 7.3 SELECTING THE MEASU-
RING METHOD, page 56).

Sample subtraction

Ion type S
continue

01.06.2016 08:00

2. If necessary, set the ion type for the ion in the standard (Ion type) with
<> <> and <ENTER>.
On selection of the ion type that can be defined (ION):
Using <> <> and <ENTER>, set the valence (Valency) and molar
weight (Molar mass) for the ion in the standard solution.
3. Select continue with <> <> and confirm with <ENTER>.

Calibration
4. Prepare two standard calibration solutions.
5. Perform a two-point calibration according to user guidance.
6. The calibration record is displayed as soon as a stable value is
achieved for the second standard calibration solution.
CALIBRATION
Z600
B092500013

01.06.2016 08:00

#1 0.010 mg/l 374.4 mV 25.0 °C

#2 0.020 mg/l 358.1 mV 25.0 °C
Slp.: 54.1 mV
Sensor +++ (Ion type: S)

01.06.2016 08:00

64 ba77147e01 07/2016
ProLab 2500 Ion concentration

Measuring
7. Start the measurement with <F1> continue.
An entry window appears.
Sample subtraction

i Immerse sensor in standard

Std. volume 100.0 ml

Std. conc. 1.0 mg/l
ISA/TISAB vol. 1.0 ml
continue

01.06.2016 08:00

8. Prepare the standard solution.

9. If necessary, add the ISA/TISAB solution to the standard solution.
10. Thoroughly rinse the electrode with deionized water.
11. Immerse the electrode in the standard solution (complexing agent or
precipitating agent).
12. Using <> <> and <ENTER>, enter the values for the volume of the
standard solution (Std. volume), the concentration of the standard solu-
tion (Std. conc.) and the volume of the ISA/TISAB solution (ISA/TISAB
vol.).
13. Select continue with <> <> and start the measurement with
<ENTER>.
When the measurement is finished an entry window appears.
Sample subtraction

i Add sample!

Sample volume 1.0 ml

continue

01.06.2016 08:00

14. Add the sample to the standard (complexing agent or precipitating

agent).
15. Using <> <> and <ENTER>, enter the value for the volume of the
sample (Sample volume).
16. Select continue with <> <> and start the measurement with
<ENTER>.
The measurement result is displayed when the measurement is com-
pleted.

ba77147e01 07/2016 65
Ion concentration ProLab 2500

Sample subtraction

01.06.2016 08:00

17. If necessary, start measuring further samples with <ENTER>.

Repeat steps 8 - 16 for all samples.
18. Terminate the measuring method with <M>.
A safety query appears.
19. Select yes with <> <>.
20. Confirm yes with <ENTER>.
Measurement with the selected method is completed.

7.3.5 Standard addition with blank value correction (Blank value

addition)

In the "Standard addition with blank value correction" procedure, a known

amount of standard solution is added to the sample in two steps.
With the first addition, the ion concentration is raised to the linear range of the
electrode characteristic curve.
The second addition is equivalent to the standard addition. The ion concentra-
tion in the sample is calculated from the change in potential.

1. Select the measuring method (see section 7.3 SELECTING THE MEASU-
RING METHOD, page 56).

Calibration
2. Prepare two standard calibration solutions.
3. Perform a two-point calibration according to user guidance.
4. The calibration record is displayed as soon as a stable value is
achieved for the second standard calibration solution.

66 ba77147e01 07/2016
ProLab 2500 Ion concentration

CALIBRATION
Z600
B092500013

01.06.2016 08:00

#1 0.010 mg/l 20.2 mV 25.0 °C

#2 0.020 mg/l 79.2 mV 25.0 °C
Slp.: 54.1 mV
Sensor +++ (Ion type: Ag)

01.06.2016 08:00

Measuring
5. Start the measurement with <F1> continue
An entry window appears.
Blank value addition

i Immerse sensor in sample

Sample volume 100.0 ml

ISA/TISAB vol. 1.0 ml
BV volume 100.0 ml
BV conc. 1.000 mg/l
continue

01.06.2016 08:00

6. Prepare the sample.

7. If necessary, add the ISA/TISAB solution to the sample.
8. Thoroughly rinse the electrode with deionized water.
9. Immerse the electrode in the sample that was supplemented with blank
value solution.
10. Using <> <> and <ENTER>, enter the values for the volume of the
sample (Sample volume), the volume of the ISA/TISAB solution (ISA/
TISAB vol.), the volume of the blank value solution (BV volume) and the
concentration of the blank value solution (BV conc.).
11. Select continue with <> <> and start the measurement with
<ENTER>.
When the measurement is finished an entry window appears.

ba77147e01 07/2016 67
Ion concentration ProLab 2500

Blank value addition

i Add standard!

Std. volume 100.0 ml

Std. conc. 1.0 mg/l
continue

01.06.2016 08:00

12. Add the standard solution to the sample.

13. Using <> <> and <ENTER>, enter the values for the volume of the
standard solution (Std. volume) and concentration of the standard solu-
tion (Std. conc.).
14. Select continue with <> <> and start the measurement with
<ENTER>. The measurement result is displayed when the measure-
ment is completed.
Blank value addition

01.06.2016 08:00

15. If necessary, start measuring further samples with <ENTER>.

Repeat steps 6 - 14 for all samples.
16. Terminate the measuring method with <M>.
A safety query appears.
17. Select yes with <> <>.
18. Confirm yes with <ENTER>.
Measurement with the selected method is completed.

68 ba77147e01 07/2016
ProLab 2500 Dissolved oxygen (D.O.)

8 Dissolved oxygen (D.O.)

8.1 Measuring

8.1.1 Measuring D.O.

1. Connect the IDS D.O. sensor to the meter.

The D.O. measuring screen is displayed.
2. If necessary, select the measured parameter with <M>.
3. Check or calibrate the meter with the sensor.

4. Immerse the D.O. sensor in the test sample.

Info 01.06.2016 08:00

Selecting the You can switch between the following displays with <M>:
displayed  D.O. concentration [mg/l]
measured parameter
 D.O. saturation [%]
Salinity correction When measuring the D.O. concentration of solutions with a salt content of more
than 1 , a salinity correction is required. For this, you have to measure and input
the salinity of the measured medium first.
When the salinity correction is switched on, the [Sal] indicator is displayed in
the measuring screen.

You can switch the salinity correction on or off and enter the salinity
in the menu for calibration and measurement settings (see section
10.4.1 SETTINGS FOR D.O. MEASUREMENT, page 88).

Air pressure The integrated air pressure sensor of the ProLab 2500 measures the current
correction air pressure.

Stability control The stability control function (AutoRead) continually checks the stability of the
(AutoRead) measurement signal. The stability has a considerable impact on the reproduc-
& HOLD function

ba77147e01 07/2016 69
Dissolved oxygen (D.O.) ProLab 2500

ibility of measured values.

The measured parameter flashes on the display
 as soon as the measured value is outside the stability range
 when the automatic Stability control is switched off.

Irrespective of the setting for automatic Stability control (see section 10.6.3
AUTOMATIC STABILITY CONTROL, page 92) in the System menu, you can start a
measurement with Stability control manually at any time.

1. Freeze the measured value with <AR>.

The [HOLD] status indicator is displayed.
The HOLD function is active.

You can terminate the Stability control function and the HOLD func-
tion with <AR> or <M> at any time.

2. Using <ENTER>, activate the Stability control function manually.

The [AR] status indicator appears while the measured value is assessed
as not stable. A progress bar is displayed and the display of the measured
parameter flashes.
As soon as a measured value meets the stability criteria, it is frozen. The
[HOLD][AR] status indicator is displayed, the progress bar disappears
and the display of the measured parameter stops flashing.
The current measurement data is output to the interface. Measurement
data meeting the stability control criterion is marked by AR.

You can prematurely terminate the Stability control function manu-

ally with <ENTER> at any time. When the Stability control function
is prematurely terminated, the current measurement data are out-
put to the interface (PC, USB memory device or USB printer) with-
out AutoRead info.

3. Using <ENTER>, start a further measurement with stability control.

or
Release the frozen measured value again with <AR> or <M>.
The [AR] status display disappears. The display switches back to the
previous indication.

Criteria for a stable The Stability control function checks whether the measured values are stable
measured value within the monitored time interval.

Measured parameter Time interval Stability in the time

interval
D.O. concentration 20 seconds ∆ : better than 0.03 mg/l

D.O. saturation 20 seconds ∆ : better than 0.4 %

70 ba77147e01 07/2016
ProLab 2500 Dissolved oxygen (D.O.)

Measured parameter Time interval Stability in the time

interval
Temperature 15 seconds ∆ : Better than 0.5 °C

The minimum duration until a measured value is assessed as stable is the mon-
itored time interval. The actual duration is mostly longer.

8.1.2 Measuring the temperature

For reproducible D.O. measurements, it is essential to measure the tempera-

ture of the test sample.
IDS D.O. sensors measure the temperature with a temperature sensor inte-
grated in the IDS sensor.

8.2 Calibration

8.2.1 Why calibrate?

8.2.2 When to calibrate?

 When the calibration interval has expired

 When your accuracy requirements are especially high
 Routinely within the framework of the company quality assurance

8.2.3 Calibration procedures

The ProLab 2500 provides 2 calibration procedures:

 Calibration in water vapor-saturated air.
 Calibration via a comparison measurement (e.g. Winkler titration according
to DIN EN 25813 or ISO 5813). At the same time, the relative slope is
adapted to the comparison measurement by a correction factor. When the
correction factor is active, the [Factor] indicator appears in the measuring
window.

8.2.4 Calibration data

The calibration record is automatically transmitted to the interface

after calibrating.

ba77147e01 07/2016 71
Dissolved oxygen (D.O.) ProLab 2500

Displaying the The calibration record of the last calibration is to be found under the menu item,
calibration record Calibration / Calibration record. To open it in the measured value display, press
the <CAL_> key.
The calibration records of the last 10 calibrations are to be found in the menu,
Calibration / Calibration data storage / Display. To open the Calibration menu,
press the <ENTER> key in the measured value display.

Menu item Setting/func- Explanation

tion
Calibration / - Displays the calibration records.
Calibration data Further options:
storage /Display  Scroll through the calibration
records with <><>.
 Using <PRT>, output the dis-
played calibration record to
the USB-B (PC) interface or
the USB-A (USB printer)
interface .
 Using <PRT_>, output all cal-
ibration records to the USB-B
(PC) interface or the USB-A
(USB printer) interface .
 Quit the display with <ESC>.
 Switch directly to the mea-
sured value display with <M>.
Calibration / Cali- - Outputs the stored calibration
bration data stor- data to the USB-A interface
age / Output to (USB memory device/USB
USB flash drive or printer)
printer
Calibration / - Outputs the stored calibration
Calibration data data to the USB-B interface (PC)
storage / Output to
RS232/USB

Calibration After calibration, the meter automatically evaluates the current status of the
evaluation calibration. The evaluation appears on the display and in the calibration record.

FDO® 1100 Display Calibration record Relative slope

calibration
+++ S = 0.94 ... 1.06
evaluation

++ S = 0.92 ... 0.94

or
S = 1.06 ... 1.08

72 ba77147e01 07/2016
ProLab 2500 Dissolved oxygen (D.O.)

Display Calibration record Relative slope

+ S = 0.90 ... 0.92
or
S = 1.08 ... 1.10
Error Error S < 0.90
or
Error elimination (see section 14 WHAT TO DO IF..., S > 1.10
page 109)

Calibration record
CALIBRATION Ox
01.06.2016 07:43:33

FDO 1100
Ser. no. 10146858

OX 930
Sensor +++

ba77147e01 07/2016 73
Conductivity ProLab 2500

9 Conductivity
9.1 Measuring

9.1.1 Measuring the conductivity

The sensor connection and the USB-B (device) interface are gal-
vanically isolated. This facilitates interference-free measurements
also in the following cases:
 Measurement in grounded test samples
 Measurement with several sensors connected to one
ProLab 2500 in one test sample

1. Connect the conductivity sensor to the meter.

The conductivity measuring window is displayed.
The measuring cell and cell constant for the connected IDS conductivity
sensor are automatically taken over.
2. If necessary, press <M> to select the measured parameter ϰ.
3. Immerse the conductivity sensor in the test sample.

Info 01.06.2016 08:00

Selecting the You can switch between the following displays with <M>:
displayed  Conductivity [μS/cm] / [mS/cm]
measured parameter
 Resistivity [Ω·cm] / [kΩ·cm] / [MΩ·cm]
 Salinity SaL [ ]
 Total dissolved solids TDS [mg/l] / [g/l]

The factor to calculate the total dissolved solids is set to 1.00 in the factory. You
can adjust this factor to meet your requirements in the range 0.40 ... 1.00. The
factor is set in the menu for the parameter TDS.
Stability control The stability control function (AutoRead) continually checks the stability of the
(AutoRead) measurement signal. The stability has a considerable impact on the reproduc-
& HOLD function ibility of measured values.
The measured parameter flashes on the display
 as soon as the measured value is outside the stability range
 when the automatic Stability control is switched off.

74 ba77147e01 07/2016
ProLab 2500 Conductivity

You can start the Stability control manually at any time, irrespective of the set-
ting for automatic Stability control (see section 10.6.3 AUTOMATIC STABILITY
CONTROL, page 92) in the System menu.

1. Freeze the measured value with <AR>.

The [HOLD] status indicator is displayed.
The HOLD function is active.

You can terminate the Stability control function and the HOLD func-
tion with <AR> or <M> at any time.

2. Using <ENTER>, activate the Stability control function manually.

The [AR] status indicator appears while the measured value is assessed
as not stable. A progress bar is displayed and the display of the measured
parameter flashes.
As soon as a measured value meets the stability criteria, it is frozen. The
[HOLD][AR] status indicator is displayed, the progress bar disappears
and the display of the measured parameter stops flashing.
The current measurement data is output to the interface. Measurement
data meeting the stability control criterion is marked by AR.

You can prematurely terminate the Stability control function manu-

ally with <ENTER> at any time. When the Stability control function
is prematurely terminated, the current measurement data are out-
put to the interface (PC, USB memory device or USB printer) with-
out AutoRead info.

3. Using <ENTER>, start a further measurement with stability control.

or
Release the frozen measured value again with <AR> or <M>.
The [AR] status display disappears. The display switches back to the
previous indication.

Criteria for a stable The Stability control function checks whether the measured values are stable
measured value within the monitored time interval.

Measured Time interval Stability in the time interval

parameter
Conductivity ϰ 10 seconds ∆ ϰ: better than
1.0% of measured value
Temperature 15 seconds ∆ : Better than 0.5 °C

The minimum duration until a measured value is assessed as stable is the mon-
itored time interval. The actual duration is mostly longer.

ba77147e01 07/2016 75
Conductivity ProLab 2500

9.1.2 Measuring the temperature

For reproducible conductivity measurements, it is essential to measure the

temperature of the test sample.
IDS sensors measure the temperature with a temperature sensor integrated in
the IDS sensor.

9.2 Temperature compensation

The calculation of the temperature compensation is based on the preset refer-
ence temperature, 20 °C or 25 °C. It appears on the display as Tr20 or Tr25.
You can select one of the following temperature compensation methods:
 Nonlinear temperature compensation (nLF) according to ISO 7888
 Linear temperature compensation (lin) with adjustable coefficients
 No temperature compensation (off)

The reference temperature and temperature compensation are set

in the menu for the parameter, conductivity (see section 10.5.1 SET-
TINGS FOR IDS CONDUCTIVITY SENSORS, page 88).

Application tips Select the following temperature compensations given in the table according to
the respective test sample:

Test sample Temperature compensation Display

Natural water nLF nLF

(ground water, surface according to ISO 7888
water, drinking water)
Ultrapure water nLF nLF
according to ISO 7888
Other aqueous solu- lin Set linear lin
tions temperature coefficient
0.000 ... 10.000 %/K
Salinity (seawater) Automatic nLF according to Sal, nLF
IOT (International Oceano-
graphic Tables)

76 ba77147e01 07/2016
ProLab 2500 Conductivity

9.3 Calibration

9.3.1 Why calibrate?

Aging slightly changes the cell constant, e. g. due to coatings. As a result, an

inexact measured value is displayed. The original characteristics of the cell can
often be restored by cleaning the cell. Calibration determines the current value
of the cell constant and stores this value in the meter.
Thus, you should calibrate at regular intervals.

9.3.2 When to calibrate?

 After connecting a sensor

 Routinely within the framework of the company quality assurance
 When the cleaning interval has expired

9.3.3 Determining the cell constant (calibration in control standard)

You can determine the actual cell constant of the IDS conductivity sensor by
calibrating with the control standard in the following range:
 0.450 ... 0.500 cm-1
(e.g. LF 413T IDS, nominal cell constant 0.475cm-1)
The cell constant is determined in the control standard, 0.01 mol/l KCl.
In the delivery condition, the calibrated cell constant of the IDS sensor is set to
0.475 cm-1 (LF 413T IDS IDS conductivity sensor).

For this calibration procedure, the Type setting must be set to cal. Proceed as
follows to determine the cell constant:

1. Connect the conductivity sensor to the meter.

2. In the measured value display, select the conductivity parameter with
<M>.
3. Start the calibration with <CAL>.
The cell constant that was calibrated last is displayed.

01.06.2016 08:00

ba77147e01 07/2016 77
Conductivity ProLab 2500

4. Immerse the conductivity sensor in the control standard solution,

0.01 mol/l KCI.
5. Start the measurement with <ENTER>.
The measured value is checked for stability (stability control).
The [AR] status indicator is displayed. The measured parameter
flashes.
6. Wait for the end of the measurement with stability control
or
take over the calibrated value with <ENTER>.
The calibration record is displayed and output to the interface.
7. Switch to the measured value display with <ENTER>.

9.3.4 Calibration data

The calibration record is automatically transmitted to the interface

after calibrating.

The calibration data can be displayed and then output to the interface.
Displaying the cali- The calibration record of the last calibration is to be found under the menu item,
bration record Calibration / Calibration record. To open it in the measured value display, press
the <CAL_> key.
The calibration records of the last 10 calibrations are to be found in the menu,
Calibration / Calibration data storage / Display. To open the Calibration menu,
press the <ENTER> key in the measured value display.

Menu item Setting/ Explanation

function
Calibration / - Displays the calibration records.
Calibration data
storage /Display Further options:
 Scroll through the calibration
records with <><>.
 Using <PRT>, output the dis-
played calibration record to the
USB-B (PC) interface or the
USB-A (USB printer) interface .
 Using <PRT_>, output all calibra-
tion records to the USB-B (PC)
interface or the USB-A (USB
printer) interface .
 Quit the display with <ESC>.
 Switch directly to the measured
value display with <M>.

78 ba77147e01 07/2016
ProLab 2500 Conductivity

Menu item Setting/ Explanation

function
Calibration / Cali- - Outputs the stored calibration data
bration data stor- to the USB-A interface (USB mem-
age / Output to ory device/USB printer)
USB flash drive or
printer
Calibration / - Outputs the stored calibration data
Calibration data to the USB-B interface (PC)
storage / Output to
RS232/USB

Calibration After calibration, the meter automatically evaluates the current status of the cal-
evaluation ibration. The evaluation appears on the display and in the calibration record.

Display Calibration record Cell constant [cm-1]

+++ Within the range
0.450 ... 0.500 cm-1

Error Error Outside the range

0.450 ... 0.500 cm-1
Error elimination (see section 14
WHAT TO DO IF..., page 109)

Calibration record
CALIBRATION Cond
01.06.2016 07:43:33

LF 413T IDS
Ser. no. 09250033

Cell constant 0.476 1/cm

25.0 °C
Sensor +++

ba77147e01 07/2016 79
Settings ProLab 2500

10 Settings
10.1 pH measurement settings

10.1.1 Settings for pH measurements

The settings are made in the menu for calibration and measurement settings of
the pH/ORP measurement. To open the settings, display the required mea-
sured parameter in the measured value display and press the <ENTER> key.
After completing the settings, switch to the measured value display with <M>.
Default settings are printed in bold.

Menu item Possible Explanation

setting
Calibration /Calibration - Displays the calibration record of the last calibration.
record
Calibration / Calibration - Displays the last calibration records (max. 10)
data storage / Display
Calibration / - Outputs the stored calibration data to the USB-B interface
Calibration data storage (PC)
/ Output to RS232/USB
Calibration / Calibration - Outputs the stored calibration data to the USB-A interface
data storage / Output to (USB memory device/USB printer)
USB flash drive or
printer
Calibration /Buffer Buffer sets to be used for pH calibration.
ConCal More buffers and details: see section 10.1.2 BUFFER SETS
NIST/DIN FOR CALIBRATION, page 82 and section 5.2 PH CALIBRATION,
... page 29.
Calibration /Single-point yes Quick calibration with 1 buffer
calibration no
Calibration /Calibration 1 ... 7 ... 999 Calibration interval for the IDS pH sensor (in days).
interval d The meter reminds you to calibrate regularly by the flash-
ing sensor symbol in the measuring screen.
Calibration /Unit for mV/pH Unit of the slope.
slope % The % display refers to the Nernst slope of
-59.2 mV/pH (100 x determined slope/Nernst slope).
QSC /First calibration - Starts the initial calibration with QSC buffers.
This menu item is only available as long as no initial cali-
bration was carried out with the connected IDS sensor.
QSC /Record of first cal- - Displays the calibration record of the QSC initial calibra-
ibration tion.
QSC /Control calibra- - Starts the control calibration with QSC buffers.
tion This menu item is only available if an initial calibration was
carried out with the connected IDS sensor.

80 ba77147e01 07/2016
ProLab 2500 Settings

Menu item Possible Explanation

setting
Alternative temperature on Takes the measured temperature value from an IDS sen-
off sor.
This menu item is only available if an IDS adapter and an
IDS sensor with temperature sensor are connected.
Temperature from ▭▭▬ Selection of the channel from which the temperature value
channel ▭▬▭ should be taken over.
▬▭▭ This menu item is only available if two IDS sensors are
additionally connected.
Man. temperature -25 ... +25 ... Entry of the manually determined temperature.
+130 °C This menu item is only available if an IDS adapter is con-
nected.
pH resolution 0.001 Resolution of the pH display
0.01
0.1
mV resolution 0.1 Resolution of the mV display
1
Limit monitoring With the Limit monitoring function you define the mea-
sured values whose exceeding is signaled. An acoustic
signal sounds, and at the same time a message is output
to the USB interface.
You can switch on or off the acoustic signal in the System
menu (see section 10.6.1 SYSTEM, page 91).
Limit monitoring/ on Switch on or off the limit signaling device for the pH value.
pH monitoring off
Limit monitoring/ on Switch on or off the limit signaling device for the tempera-
TP monitoring off ture value.
Limit monitoring/ -2 ... 20 Upper limit. A message is output to the USB interface if it
pH monitoring/ is exceeded.
on/ This menu item is only visible when the pH monitoring set-
pH upper limit ting is active.
Limit monitoring/ -2 ... 20 Lower limit. A message is output to the USB interface if it
pH monitoring/ is exceeded.
on/ This menu item is only visible when the pH monitoring set-
pH lower limit ting is active.
Limit monitoring/ -5 ... Upper limit. A message is output to the USB interface if it
TP monitoring/ +105 °C is exceeded.
on/ This menu item is only visible when the TP monitoring set-
TP upper limit ting is active.
Limit monitoring/ -5 ... 105 °C Lower limit. A message is output to the USB interface if it
TP monitoring/ is exceeded.
on/ This menu item is only visible when the TP monitoring set-
TP lower limit ting is active.

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Settings ProLab 2500

Menu item Possible Explanation

setting
Reset - Resets all sensor settings to the delivery condition (see
section 10.7.1 RESETTING THE MEASUREMENT SETTINGS,
page 93).

10.1.2 Buffer sets for calibration

You can use the buffer sets quoted in the table for an automatic calibration. The
pH values are valid for the specified temperature values. The temperature
dependence of the pH values is taken into consideration during the calibration.

No. Buffer set * pH values at

1 ConCal Any Any
2 NIST/DIN 1.679 25 °C
DIN buffers according to DIN 19266 4.006
and NIST Traceable Buffers 6.865
9.180
12.454
3 TEC 2.000 25 °C
Technical buffers 4.010
7.000
10.011
4 Merck 1* 4.000 20 °C
7.000
9.000
5 Merck 2 * 1.000 20 °C
6.000
8.000
13.000
6 Merck 3 * 4.660 20 °C
6.880
9.220
7 Merck 4 * 2.000 20 °C
4.000
7.000
10.000
8 Merck 5 * 4.010 25 °C
7.000
10.000
9 DIN 19267 1.090 25 °C
4.650
6.790
9.230

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No. Buffer set * pH values at

10 Mettler Toledo USA * 1.679 25 °C
4.003
7.002
10.013
11 Mettler Toledo EU * 1.995 25 °C
4.005
7.002
9.208
12 Fisher * 2.007 25 °C
4.002
7.004
10.002
13 Fluka BS * 4.006 25 °C
6.984
8.957
14 Radiometer * 1.678 25 °C
4.005
7.000
9.180
15 Baker * 4.006 25 °C
6.991
10.008
16 Metrohm * 3.996 25 °C
7.003
8.999
17 Beckman * 4.005 25 °C
7.005
10.013
18 Hamilton Duracal * 4.005 25 °C
7.002
10.013
19 Precisa * 3.996 25 °C
7.003
8.999
20 Reagecon TEC * 2.000 25 °C
4.010
7.000
10.000
21 Reagecon 20 * 2.000 20 °C
4.000
7.000
10.000
13.000

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Settings ProLab 2500

No. Buffer set * pH values at

22 Reagecon 25 * 2.000 25 °C
4.000
7.000
10.000
13.000
23 Chemsolute * 2.000 20 °C
4.000
7.000
10.000
24 USABlueBook * 4.000 25 °C
7.000
10.000

* Brand names or trade names are trademarks of their respective owners pro-
tected by law.

The buffers are selected in the menu, pH / <ENTER> / Calibration

/ Buffer (see section 10.1.1 SETTINGS FOR PH MEASUREMENTS, page
80).

10.1.3 Calibration interval

The calibration evaluation is displayed as a sensor symbol.

After the QSC function has been enabled the sensor symbol is replaced by the
QSC scale (see section 5.2.8 QSC FUNCTION (SENSOR QUALITY CONTROL), page
40).
After the specified calibration interval has expired the sensor symbol or the
QSC scale flashes. It is still possible to measure.

To ensure the high measuring accuracy of the measuring system,

calibrate after the calibration interval has expired.

Setting the calibra- The calibration interval is set to 7 days in the factory.
tion interval You can change the interval (1 ... 999 days):

1. Open the menu for measurement settings with <ENTER>.

2. In the Calibration / Calibration interval menu, set the calibration interval
with <><>.
3. Confirm the setting with <ENTER>.
4. Quit the menu with <M>.

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10.2 ORP measurement settings

Settings The settings are made in the menu for measuring settings of the ORP measure-
ment. To open the settings, display the required measured parameter in the
measured value display and press the <ENTER> key. After completing the set-
tings, switch to the measured value display with <M>.
Default settings are printed in bold.

Menu item Possible setting Explanation

mV resolution 0.1 Resolution of the mV display
1
Reset - Resets all sensor settings to the
delivery condition (see section
10.7.1 RESETTING THE MEASURE-
MENT SETTINGS, page 93).

10.3 ISE measurement settings

The settings are made in the measuring menu of the ISE measurement. To
open the settings, activate the relevant measuring window in the measured
value display and press the <ENTER> key shortly. After completing the set-
tings, switch to the measured value display with <M>.
The following settings are possible for ISE measurements:

Menu item Possible setting Description

Calibration / - Displays the calibration
Calibration record record of the last calibration.
Calibration / - Displays the calibration
Calibration data stor- record.
age /
Display Further options:
 Scroll through the cali-
bration records with
<><>.
 Output the displayed cal-
ibration record to the
interface with <F2>/
[USB-Ausgabe].
 Output all calibration
records to the interface
with <F2_>[USB-Aus-
gabe].
 Quit the display with
<F1>/[Back] or
<ENTER>.
 Switch directly to the
measured value display
with <M>.

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Settings ProLab 2500

Menu item Possible setting Description

Calibration / - Outputs the calibration
Calibration data stor- records to the interface.
age /
Output to RS232/
USB
Man. temperature -25 ... +25 ... +130 °C Entry of the manually deter-
mined temperature. For
measurements without tem-
perature sensor only.
Alternative tempera- on Takes the measured tem-
ture off perature value from an IDS
sensor.
This menu item is only avail-
able if an IDS adapter and
an IDS sensor with temper-
ature sensor are connected.
Temperature from ▭▭▬ Selection of the channel
channel ▭▬▭ from which the temperature
▬▭▭ value should be taken over.
This menu item is only avail-
able if two IDS sensors are
additionally connected.
ISE setup / low Selection of the AutoRead
AutoRead criterion medium criteria (see section 7.1.1
high MEASURING THE ION CON-
CENTRATION, page 47).

ISE setup / Ag, Br, Ca, Cd, Cl, Selection of the ion type to
Ion type CN, Cu, F, I, K, Na, be measured.
NO3, Pb, S, NH3,
NH4*, CO2, ION An ion that is not included in
the list can be measured
with the setting, ION.
* Measuring with the NH 500 electrode:
The NH4 setting is not suitable for the gas-sensitive
electrode NH 500.
Select the following settings:
Ion type "ION", Valency "-1".
ISE setup / mg/l Selection, with which unit
Unit µmol/l the measurement result and
mg/kg calibration standards should
ppm be displayed.
%
ISE setup/ -8 ... +8 Set the valence (Valency)
Valency and molar weight (Molar
mass) for the ion (only with
ISE setup/ 1 ... 300 g/mol Unit: mg/kg, ppm, %)
Molar mass

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Menu item Possible setting Description

ISE setup/ 0.001 ... 9.999 g/ml or Adjustable density of the
Density kg/l test sample (only with Unit:
mg/kg, ppm, %)
Method Standard addition Selection of the available
Standard subtraction measuring methods.
Sample addition
Sample subtraction
Blank value addition
Start method Start measurement with the
selected method.

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Settings ProLab 2500

10.4 D.O. measurement settings

10.4.1 Settings for D.O. measurement

The settings are available in the menu for measurement and calibration set-
tings. To open the settings, display the required measured parameter in the
measured value display and press the <ENTER> key. After completing the set-
tings, switch to the measured value display with <M>.

10.5 Cond measurement settings

10.5.1 Settings for IDS conductivity sensors

The settings are made in the menu for the measured parameter, conductivity.
To open the settings, display the required measured parameter in the mea-
sured value display and press the <ENTER> key. After completing the settings,
switch to the measured value display with <M>.
The possible settings are individually displayed for each sensor. Below the set-
ting menu is displayed for two IDS sensors (LF 413T IDS, LF 313T IDS).
Default settings are printed in bold.

Setting menu of Menu item Possible Explanation

LF 413T IDS setting
Calibration /Calibra- - Displays the calibration record of the
tion record last calibration.
Calibration / Calibra- - Displays the last calibration records
tion data storage / (max. 10)
Display
Calibration / Calibra- - Outputs the stored calibration data to
tion data storage / the USB-A interface (USB memory
Output to USB flash device/USB printer)
drive or printer
Calibration / - Outputs the stored calibration data to
Calibration data stor- the USB-B interface (PC)
age / Output to
RS232/USB
Calibration /Calibra- 1 ... 150 ... Calibration interval for the IDS con-
tion interval 999 d ductivity sensor (in days).
The meter reminds you to calibrate
regularly by the flashing sensor sym-
bol in the measuring screen.

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Menu item Possible Explanation

setting
Type Used measuring cell
Cal Measuring cells whose cell constant
is determined by calibration in the KCl
control standard solution.
Calibration range:
0.450 to 0.500 cm-1
The currently valid cell constant is dis-
played in the status line.
man Freely adjustable cell constant in the
range 0.450 ... 0.500 cm-1.
Man. cell const. 0.450 ... Display and setting options for the cell
0.475 ... constant.
0.500 cm-1
Temp. comp. (TC) / nLF Procedure for temperature compen-
Method lin sation (see section 9.2 TEMPERATURE
off COMPENSATION, page 76).
This setting is only available for the
measured parameters, ϰ and ρ.
Temp. comp. (TC) / 0.000 ... Coefficient of the linear temperature
Linear coeff. 2.000 ... compensation.
3.000 %/K This menu item is only available when
the linear temperature compensation
is set.
Temp. comp. (TC) / 20 °C Reference temperature
Reference temp. 25 °C This setting is only available for the
measured parameters, ϰ and ρ.
Multiplier for TDS 0.40 ... 1.00 Factor for TDS value
Reset - Resets all sensor settings to the deliv-
ery condition (see section 10.7.1
RESETTING THE MEASUREMENT SET-
TINGS, page 93).

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Settings ProLab 2500

Setting menu of Menu item Possible Explanation

LF 313T IDS setting
Cell constant 0.090 ... ... Display and setting options for the
0.100 ... cell constant
0.110 cm-1
Temp. comp. (TC) / nLF Procedure for temperature compen-
Method lin sation (see section 9.2 TEMPERA-
off TURE COMPENSATION, page 76).
This setting is only available for the
measured parameters, ϰ and ρ.
Temp. comp. (TC) / 0.000 ... Coefficient of the linear temperature
Linear coeff. 2.000 ... compensation.
3.000 %/K This menu item is only available
when the linear temperature com-
pensation is set.
Temp. comp. (TC) / 20 °C Reference temperature
Reference temp. 25 °C This setting is only available for the
measured parameters, ϰ and ρ.
Multiplier for TDS 0.40 ... 1.00 Factor for TDS value
Reset - Resets all sensor settings to the
delivery condition (see section
10.7.1 RESETTING THE MEASUREMENT
SETTINGS, page 93).

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10.6 Sensor-independent settings

10.6.1 System

To open the Storage & config menu, press the <ENTER_> key in the measured
value display. After completing the settings, switch to the measured value dis-
play with <M>.
Default settings are printed in bold.

Menu item Setting Explanation

System / General / Language Deutsch Selects the menu language
English
(more)
System / General / Audio signal on Switches on/off the beep on key-
off stroke
System / General / Brightness 0 ... 10 ... 22 Changes the display brightness
System / General / Tempera- °C Temperature unit,
ture unit °F degrees Celsius or degrees Fahren-
heit.
All temperature values are displayed
with the selected unit.
System / General / Stability on Switches on or off the automatic sta-
control off bility control during measurement
(see section 10.6.3 AUTOMATIC STA-
BILITY CONTROL, page 92 )

System / Interface / Baud rate 1200, 2400, 4800, Baud rate of the USB Device inter-
9600, 19200 face
System / Interface / Output for- ASCII Output format for data transmission
mat CSV For details, see section 12 TRANSMIT-
TING DATA, page 102

System / Interface / Decimal Dot (xx.x) Decimal separator

separator Comma (xx,x)
System / Interface / Output Output of a header for Output format:
header CSV
System /Clock function Date format Settings of time and date.
Datum For details, see section 4.5.5
Time EXAMPLE 2 ON NAVIGATION: SETTING
THE DATE AND TIME, page 25

System /Service information Hardware version and software ver-

sion of the meter are displayed.
System /Reset - Resets the system settings to the
default values.
For details, see section 10.7.2
RESETTING THE SYSTEM SETTINGS,
page 95

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Settings ProLab 2500

10.6.2 Data storage

This menu contains all functions to display, edit and erase stored measured
values.

Detailed information on the memory functions of the ProLab 2500

are given in section 11 DATA STORAGE, page 96.

10.6.3 Automatic Stability control

The automatic Stability control (AutoRead) function continuously checks the

stability of the measurement signal. The stability has a considerable impact on
the reproducibility of measured values.
You can activate or switch off the automatic Stability control function (see
section 10.6 SENSOR-INDEPENDENT SETTINGS, page 91).
The measured parameter flashes on the display
 as soon as the measured value is outside the stability range
 when you switch over between the measured parameters with <M>.
 when the automatic Stability control is switched off.

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10.7 Reset
You can reset (initialize) all sensor settings and sensor-independent settings
separately from each other.

10.7.1 Resetting the measurement settings

The calibration data are reset to the default settings together with
the measuring parameters. Recalibrate after performing a reset.

pH The following settings for pH measurements are reset to the default settings
with the Reset function:

Setting Default settings

Buffer
Calibration interval 7d
Unit for slope mV/pH
Measured parameter pH
pH resolution 0.001
mV resolution 0.1
Asymmetry 0 mV
Slope -59.2 mV
Man. temperature 25 °C
Single-point calibration off

The sensor settings are reset under the Reset menu item in the menu for cali-
bration and measurement settings. To open the settings, display the required
measured parameter in the measured value display and press the <ENTER>
key.
ORP The following settings for ORP measurements are reset to the default settings
with the Reset function:

Setting Default settings

mV resolution 0.1
Man. temperature 25 °C

The sensor settings are reset under the Reset menu item in the menu for cali-
bration and measurement settings. To open the settings, display the required
measured parameter in the measured value display and press the <ENTER>
key.

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Settings ProLab 2500

ISE The following settings for ISE measurements are reset to the default settings
with the Reset function:

Setting Default settings

AutoRead criterion high
Ion type Ag
Unit mg/l
Man. temperature 25 °C
Alternative temperature off
Method Standard addition

The sensor settings are reset under the Reset menu item in the menu for cali-
bration and measurement settings. To open the settings, display the required
measured parameter in the measured value display and press the <ENTER>
key.
Dissolved oxygen The following settings for D.O. measurements are reset to the default settings
with the Reset function:

Setting Default settings

Calibration interval 180 d
Check interval 60 d
Measured parameter D.O. concentration
Relative slope (SRel) 1.00
Salinity (value) 0.0
Salinity (function) Off

The sensor settings are reset under the Reset menu item in the menu for cali-
bration and measurement settings. To open the settings, display the required
measured parameter in the measured value display and press the <ENTER>
key.
Conductivity The following settings for conductivity measurements are reset to the default
settings with the Reset function:

Setting Default settings

Calibration interval 150 d
Measured parameter ϰ
Cell constant (c) Depending on the connected mea-
suring cell:
0.475 cm-1 (calibrated)
0.475 cm-1 (set)
0.100 cm-1
Temperature compensation nLF

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Setting Default settings

Reference temperature 25 °C
Temperature coefficient (TC) of the 2.000 %/K
linear temperature compensation
Multiplier for TDS 1.00

The sensor settings are reset under the Reset menu item in the menu for cali-
bration and measurement settings. To open the settings, display the required
measured parameter in the measured value display and press the <ENTER>
key.

10.7.2 Resetting the system settings

The following system settings can be reset to the default condition:

Setting Default settings

Language English
Audio signal on
Baud rate 4800 Baud
Output format ASCII
Decimal separator Dot (xx.x)
Brightness 10
Temperature unit °C
Stability control on

The system settings are reset in the menu, Storage & config / System / Reset.
To open the Storage & config menu, press the <ENTER_> key in the measured
value display.

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Data storage ProLab 2500

11 Data storage
You can transmit measured values (datasets) to the data storage:
 Manual data storage (see section 11.1 MANUAL DATA STORAGE, page 96)
 Automatic data storage at intervals (see section 11.2 AUTOMATIC DATA STO-
RAGE AT INTERVALS, page 96)

11.1 Manual data storage

You can transmit a measurement dataset to the data storage as follows. The
dataset is at the same time output to the interface:

1. Press the <STO> key shortly.

The menu for manual data storage appears.

Manual data storage 4 von 500

01.06.2016 11:24:16
pH 7.000 24.8 °C AR +++

ID number: 1
continue

01.06.2016 08:00

2. If necessary, change and confirm the ID number (1 ... 10000) with

<><> and <ENTER>.
The dataset is stored. The meter switches to the measured value dis-
play.

If the storage is full When all storage locations are occupied, it is not possible to continue storing.
Then you can e.g. transmit the stored data to a PC or a USB flash drive (see
section 11.3.1 EDITING THE MEASUREMENT DATA STORAGE, page 99) and subse-
quently erase the storage (see section 11.3.2 ERASING THE MEASUREMENT DATA
STORAGE, page 100).

11.2 Automatic data storage at intervals

The storage interval (Interval) determines the time interval between automatic
data storage processes. Each data storage process transmits the current data-
set to the interface at the same time.

Configuring the
1. Press the <STO_> key.
automatic storage
The menu for automatic data storage appears.
function

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Automatic data storage

ID number 1
Interval 30 s
1
Duration 180 min
continue

0d03h00min 2

0 1d17h33min

01.06.2016 08:00

1 Adjusted entire storage duration

2 Max. available storage duration
3 Graphical display of the storage usage

Settings You can configure the automatic data storage function with the following
settings:

Menu item Possible setting Explanation

ID number 1 ... 10000 ID number for the dataset series.
Interval 1 s, 5 s, 10 s, Storage interval.
30 s, 1 min, The lower limit of the storage interval
5 min, 10 min, can be restricted by the number of free
15 min, 30 min, storage locations.
60 min The upper limit is restricted by the stor-
age duration.
Duration 1 min ... x min Storage duration.
Specifies after which time the auto-
matic data storage should be termi-
nated.
The lower limit of the storage duration
is restricted by the storage interval.
The upper limit is restricted by the
number of free storage locations.

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Data storage ProLab 2500

Starting the To start the automatic storage function, select continue with <><> and con-
automatic storage firm with <ENTER>. The meter switches to the measured value display.
function

0d03h00min

01.06.2016 08:00
2

1 Remaining storage duration

2 Graphical display of the storage duration

The active automatic data storage function can be recognized by the progress
bar in the status line. The progress bar indicates the remaining storage dura-
tion.

If the automatic data storage function is activated, only the follow-

ing keys are active: <M>, <STO_> and <On/Off>. The other keys
and the automatic switch-off function are deactivated.

Terminating the Proceed as follows to switch off the automatic data storage function before the
automatic storage adjusted storage duration has expired:
function prema-
turely
1. Press the <STO_> key.
The following window appears.

Warning

Stop automatic storage?

yes
no

01.06.2016 08:00

2. Using <><>, select yes and confirm with <ENTER>.

The meter switches to the measured value display.
The automatic data storage function is terminated.

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11.3 Measurement data storage

11.3.1 Editing the measurement data storage

The contents of the manual or automatic measurement data storage can be

shown on the display.
Each of the measurement data storages has a function to erase the entire
contents.
Editing the The storage is edited in the menu, Storage & config / Data storage. To open
data storage the Storage & config menu, press the <ENTER_> key in the measured value
display.
Open the manual or automatic storage directly with the <RCL> or <RCL_> key.

The settings are explained here using the manual data storage as
an example. The same settings and functions are available for the
automatic data storage.

Settings Menu item Setting/ Explanation

function
Data storage / - Displays all measurement datas-
Manual data storage / ets page by page.
Display
Further options:
 Scroll through the datasets
with <><>.
 Output the displayed dataset
to the interface with <PRT>.
 Quit the display with <ESC>.
Data storage / - Outputs all stored measured val-
Manual data storage / ues to the USB-A interface (USB
Output to USB flash memory device/USB printer)
drive or printer
Data storage / - Outputs all stored measurement
Manual data storage / data to the USB-B interface (PC)
Output to RS232/USB
Data storage / - Erases the entire manual mea-
Manual data storage / surement data storage.
Erase Note:
All calibration data remain stored
when this action is performed.

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Data storage ProLab 2500

Display presentation Manual data storage 3 of 64

of a dataset 01.06.2016 11:24:16 ID number: 1

BlueLine 24 pH IDS B20294008565

pH 7.000 24.8 °C AR Sensor: +++

01.06.2016 08:00

Sample printout
01.06.2016 09:56:20
ProLab 2500
Ser. no. 09250023

BlueLine 24 pH IDS
Ser. no. B092500013
ID number 2
pH 6.012 24.8 °C, AR, Sensor: +++
________________________________________

01.06.2016 10:56:20
ProLab 2500
Ser. no. 09250013

BlueLine 24 pH IDS
Ser. no. B092500013
ID number 2
pH 6.012 24.8 °C, AR, Sensor: +++
________________________________________

etc...

Quitting the display To quit the display of stored measurement datasets, you have the following
options:
 Switch directly to the measured value display with <M>.
 Quit the display and move to the next higher menu level with <ESC>.

11.3.2 Erasing the measurement data storage

How to erase the measured value storage is described in section 11.3.1

EDITING THE MEASUREMENT DATA STORAGE, page 99.

11.3.3 Measurement dataset

A complete dataset includes:

 Date/time
 Meter name, series number
 Sensor name, series number
 ID number

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ProLab 2500 Data storage

 Measured value of the connected sensor

 Measured temperature value of the connected sensor
 AutoRead info: AR appears with the measured value if the AutoRead crite-
rion was met while storing (stable measured value). Otherwise, the AR dis-
play is missing.
 Calibration evaluation:
- 4 levels (+++, ++, +, -, or no evaluation) or
- QSC (percentage)

11.3.4 Storage locations

The ProLab 2500 meter has two measurement data storages. The measured
values recorded either manually or automatic are stored separately in indi-
vidual measurement data storages.

Storage Maximum number of datasets

Manual data storage 500
Automatic data storage 10000

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Transmitting data ProLab 2500

12 Transmitting data
12.1 Outputting current measurement data

1. Output the current measurement data to an interface with <PRT>.

 USB-B: e.g.: PC
 USB-A: e.g.: printer or USB flash drive
(if no PC is connected)

12.2 Transmitting data

The meter has the following interfaces:
 USB-B interface (USB Device)
e.g. to connect a PC
 USB-A interface (USB Host),
e.g. to connect a USB flash drive/USB printer

Via the USB-B interface (USB Device) you can transmit data to a PC or printer
and update the meter software.
Via the USB-A interface (USB Host) it is possible to transfer data to an external
USB memory device/USB printer.

12.3 Connecting the PC / USB-B interface (USB Device)

Connect the ProLab 2500 to the PC via the USB-B interface.
Installation of the System requirements of the PC for installation of the USB driver:
USB driver on the
PC  PC with at least one free USB connection and CD-ROM drive
 Windows 7/8/8.1/10.

1. Insert the supplied installation CD in the CD drive of your PC.

2. Install the driver from the CD.
Follow the Windows installation instructions as necessary.
3. Connect the ProLab 2500 to the PC via the USB-B interface.
The meter is listed as a virtual COM interface among the connections in
the Windows instrument manager.

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ProLab 2500 Transmitting data

4. Set the same transmission data at the connected instrument (PC):

 Baud rate: to be selected in the range 1200 ... 19200
 Handshake: RTS/CTS
 Set at the PC only:
– Parity: none
– Data bits: 8
– Stop bits: 2

12.4 Connecting a USB memory device/USB printer

(USB-A interface (USB Host))
Connect the USB-A interface (USB Host) of the ProLab 2500 to a USB flash
drive/USB printer.

1. Connect a USB memory device/USB printer to the USB Host interface.

Suitable USB printers:

Model Type Paper width

Citizen CT-S281 Thermal transfer printer 58 mm
Seiko Instruments Inc. Thermal transfer printer 58 mm
DPU-S445
Star SP700 with USB Matrix printer 76 mm
interface*

* Recommended printer settings for Star SP700:

- CodePage 437
- DIP switch 1...7: =ON, DIP switch 8: OFF
Details: see operating manual of your printer.

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Transmitting data ProLab 2500

12.5 Options for data transmission to the USB-B (PC)

interface and the USB-A (USB printer) interface
Via the USB-B interface you can transmit data to a PC.
Via the USB-A interface, you can transmit data to a USB printer or USB mem-
ory device. How to transmit data to a USB memory device is described in an
extra section (see section 12.6 DATA TRANSMISSION TO THE USB-A INTERFACE
(USB MEMORY DEVICE), page 105).
The following table shows which data are transmitted to the interface in which
way:

Data Control Operation / description

Current Manual  With <PRT>.
measured  Simultaneously with every manual data storage process (see
values of all section 11.1 MANUAL DATA STORAGE, page 96).
connected
sensors Automatic, at  With <PRT_>.
intervals Then you can set the transmission interval.
 Simultaneously with every automatic data storage process (see
section 11.2 AUTOMATIC DATA STORAGE AT INTERVALS, page 96).
Stored mea- Manual  Displayed dataset with <PRT> after calling up from the storage.
sured values  All datasets with the Output to RS232/USB function.
(see section 11.3.1 EDITING THE MEASUREMENT DATA STORAGE,
page 99).
 All datasets with the Output to USB flash drive or printer function
(USB-A interface)
For details, see section 11.3.1 EDITING THE MEASUREMENT DATA
STORAGE, page 99.
Calibration Manual  Displayed calibration record with <PRT> (see section 5.2.6 CALI-
records BRATION DATA, page 36; section 8.2.4 CALIBRATION DATA, page
71; section 9.3.4 CALIBRATION DATA, page 78).
 All calibration records with the Output to USB flash drive or
printer function.
 All calibration records with <PRT_>.
For details, see section 12 TRANSMITTING DATA, page 102.
Automatic  At the end of a calibration procedure.

The following rule applies: With the exception of the menus, shortly
pressing the <PRT> key generally outputs the display contents to
the interface (displayed measured values, measurement datasets,
calibration records).

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ProLab 2500 Transmitting data

12.6 Data transmission to the USB-A interface

(USB memory device)
Via the USB-A interface, you can transmit data to a USB memory or USB
printer device. How to transmit data to a USB printer device is described in an
extra section (see section 12.6 DATA TRANSMISSION TO THE USB-A INTERFACE
(USB MEMORY DEVICE), page 105).
The following table shows which data are transmitted to the interface in which
way:

Data Control Operation / description

Stored mea- Manual All datasets with the Output to RS232/
sured values USB function.
For details, see section 11.3.1 EDITING
THE MEASUREMENT DATA STORAGE, page
99
Calibration Manual All stored calibration records of a sensor
data storage with the Calibr. data storage to USB flash
drive function.
For details, see menu for calibration and
measurement settings of the sensor.

12.7 MultiLab Importer

With the aid of the MultiLab Importer software, you can record and evaluate
measurement data with a PC.

More detailed information can be found in the MultiLab Importer

operating manual.

With the aid of the BOD Analyst Pro software you can administrate BOD
measurement on the PC and automatically calculate the measured values.

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Maintenance, cleaning, disposal ProLab 2500

13 Maintenance, cleaning, disposal

13.1 Maintenance

13.1.1 General maintenance activities

The only maintenance activity required is exchanging the battery that buffers
the system clock.

See the relevant operating manuals of the IDS sensors for instruc-
tions on maintenance.

13.1.2 Exchanging the battery

The ProLab 2500 has a battery (CR2032 type) to buffer the system clock in
case of a power failure.

To retain the current setting of the date and time during the battery
exchange, supply the meter with power with the power pack while
changing the battery.

To avoid that the system clock is reset in the case of a power failure,
we recommend changing the battery before the end of its service
life (the battery included in the scope of delivery after approx. 8
years).

1. Unscrew the screws (2) of the lid of the battery compartment with a
screwdriver.

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ProLab 2500 Maintenance, cleaning, disposal

2. Open the battery compartment (1) on the underside of the meter.

3. Remove the battery from the battery compartment.
4. Insert a new battery in the battery compartment.
5. Close the battery compartment (1).
The date (day) flashes on the display.
6. Tighten the screws (2) of the lid of the battery compartment with a
screwdriver.
7. Set the date and time (see 4.5.5 EXAMPLE 2 ON NAVIGATION: SETTING
THE DATE AND TIME, PAGE 25)

Dispose of used batteries according to the local regulations of your

country.
End users within the European Union are obligated to return used
batteries (even ecologically compatible ones) to a collection point
set up for recycling purposes.
Batteries are marked with the crossed-out waste container
symbol. Therefore, they may not be disposed with the domestic
waste.

13.2 Cleaning
Occasionally wipe the outside of the measuring instrument with a damp, lint-
free cloth. Disinfect the housing with isopropanol as required.

CAUTION
The housing is made of synthetic material (ABS). Thus, avoid
contact with acetone or similar detergents that contain sol-
vents. Remove any splashes immediately.

13.3 Packing
This meter is sent out in a protective transport packing.
We recommend: Keep the packing material. The original packing protects the
meter against damage during transport.

13.4 Disposal
At the end of its operational lifetime, the meter must be returned to the
disposal or return system statutory in your country. If you have any questions,
please contact your supplier.

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Maintenance, cleaning, disposal ProLab 2500

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ProLab 2500 What to do if...

14 What to do if...
14.1 pH

More information and instructions on cleaning and exchange of

sensors are given in the documentation of your sensor.

Error message Cause Remedy

OFL, UFL
IDS pH sensor:
– Measured value outside the measur- – Use suitable IDS pH sensor
ing range
– Air bubble in front of the diaphragm – Remove air bubble
– Air in the diaphragm – Extract air or moisten junction
– Cable broken – Exchange IDS pH sensor
– Gel electrolyte dried out – Exchange IDS pH sensor

Error message, Cause Remedy

Error
IDS pH sensor:
– The values determined for zero point – Recalibrate
and slope of the IDS pH sensor are
outside the allowed limits.
– Diaphragm contaminated – Clean diaphragm
– IDS pH sensor broken – Exchange IDS pH sensor
Buffer solutions:
– The used buffer solutions do not – Set different buffer set
agree with the set buffer set or
– Use different buffer solutions
– Buffer solutions too old – Use only once.
Note the shelf life
– Buffer solutions depleted – Change solutions

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What to do if... ProLab 2500

No stable measured Cause Remedy

value
IDS pH sensor:
– Diaphragm contaminated – Clean diaphragm
– Membrane contaminated – Clean membrane

Test sample:
– pH value not stable – Measure with air excluded if
necessary
– Temperature not stable – Adjust temperature if necessary

IDS pH sensor + test sample:

– Conductivity too low – Use suitable IDS pH sensor
– Temperature too high – Use suitable IDS pH sensor
– Organic liquids – Use suitable IDS pH sensor

Obviously incorrect Cause Remedy

measured values
IDS pH sensor:
– IDS pH sensor unsuitable – Use suitable IDS sensor
– Temperature difference between – Adjust temperature of buffer or
buffer and test sample too great sample solutions
– Measurement procedure not suit- – Follow special procedure
able

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ProLab 2500 What to do if...

14.2 ISE

Error message OFL Cause Remedy

– Measuring range exceeded – Dilute the test sample

Obviously incorrect Cause Remedy

measured values
– Electrode not connected – Connect the electrode
– Cable broken – Replace the electrode

Error message, Cause Remedy

Error (invalid
calibration) ISE electrode:
or – Moisture in the plug – Dry the plug
Calibration
evaluation poor (-) – Electrode is old – Replace the electrode
– Electrode not suitable for the range – Use a suitable electrode
to be measured
– Electrode not suitable for the – Use a suitable electrode or
selected ion select a suitable ion
– Socket damp – Dry the socket
Calibration procedure:
– Wrong sequence of standards for – Select the correct sequence
three- to seven-point calibration
– Calibration standards do not have – Adjust the temperature of the
the correct temperature (max. ± 2 °C calibration standards
temperature difference)

Warning [TpErr] Cause Remedy

– Temperature difference between – Adjust the temperature of the
measurement and calibration test sample
greater than 2 °C.

Warning [ISEErr] Cause Remedy

– Electrode voltage outside calibrated – Recalibrate
range

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What to do if... ProLab 2500

14.3 Dissolved oxygen

More information and instructions on cleaning and exchange of

sensors are given in the documentation of your sensor.

Error message, Cause Remedy

OFL
– Measured value outside the measur- – Select a suitable measuring
ing range medium

Error message, Cause Remedy

Error
– Sensor contaminated – Clean the sensor
– Measured temperature value outside – Keep to the temperature range
the operating conditions for the test sample
(display of OFL/UFL instead of a
temperature value)
– Defective sensor – Replace sensor

14.4 Conductivity

More information and instructions on cleaning and exchange of

sensors are given in the documentation of your sensor.

Error message, Cause Remedy

OFL
– Measured value outside the measur- – Use suitable IDS conductivity
ing range sensor

Error message, Cause Remedy

Error
– Sensor contaminated – Clean the sensor and replace it
if necessary
– Calibration solution not suitable – Check the calibration solutions

More information and instructions on cleaning and exchange of

sensors are given in the documentation of your sensor.

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14.5 General information

Sensor symbol Cause Remedy

flashes
– Calibration interval expired – Recalibrate the measuring sys-
tem

Meter does not react Cause Remedy

to keystroke
– Operating condition undefined or – Processor reset:
EMC load unallowed Press the <ENTER> and <On/
Off> key simultaneously

You want to know Cause Remedy

which software
version is in the – E. g., a question by the service – Switch on the meter.
meter or IDS sensor department – Open the menu, <ENTER_> /
Storage & config / System / Ser-
vice information. The instrument
data are displayed.
or
– Connect the sensor.
Press softkey [Info]/[More] The
sensor data are displayed (see
section 4.1.6 SENSOR INFO,
page 17)

Data transmission to Cause Remedy

USB memory device
does not work – Connected USB memory device was – Use other USB memory device
not recognized – Disconnect the PC from the
– The USB-B interface is connected to USB-B interface
a PC – Reformat the USB memory
– The USB memory device has been device to FAT 16 or FAT 32 file
formatted to a file system which is system
not supported, e. g. NTFS (Caution: Reformatting erases
all data on the USB memory
device. Back up all data before
reformatting.)

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What to do if... ProLab 2500

Data transmission to Cause Remedy

USB printer does not
work – The USB-B interface is connected to – Disconnect the PC from the
a PC USB-B interface
– Connected USB memory device – Use a suitable USB printer (see
does not work section 12.4 CONNECTING A USB
MEMORY DEVICE/USB PRINTER
(USB-A INTERFACE (USB
HOST)), page 103)
– Check printer settings (see sec-
tion 12.4 CONNECTING A USB
MEMORY DEVICE/USB PRINTER
(USB-A INTERFACE (USB
HOST)), page 103)

Error message, Cause Remedy

Memory error 1
– Instrument storage was not found – Please contact the service.

Time gets lost Cause Remedy

– Buffer battery is empty – Change the buffer battery (see
section 13.1.1 GENERAL MAINTE-
NANCE ACTIVITIES, page 106)

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ProLab 2500 Technical data

15 Technical data
15.1 Measuring ranges, resolution, accuracy

Measuring ranges, Parameter Measuring range Accuracy

accuracy
Air pressure (abso- 300 ... 1100 mbar ± 4 mbar
lute)*

*Available only if a D.O. sensor is connected

Further data are given in the documentation of your sensor.

15.2 General data

Dimensions Approx. 180 x 80 x 55 mm

Weight Approx. 0.4 kg
Mechanical structure Type of protection IP 43
Electrical safety Protective class III
Test certificates CE (Declaration of Conformity: see www.si-analytics.com),
cETLus

Ambient conditions Storage - 25 °C ... + 65 °C

Operation 0 °C ... + 40 °C
Admissible relative Yearly mean: < 75 %
humidity 30 days/year: 95 %
Other days: 85 %

Power supply Power pack Helms-man Industrial Co Ltd SEI0901100P

Input: 100 ... 240 V ~ / 50 ... 60 Hz / 0.5 A
Output: 9 Vdc, 1100 mA
Connection max. overvoltage category II
Primary plugs contained in the scope of
delivery: Euro, US, UK and Australian.
Battery 3.0 V lithium manganese dioxide battery,
(to buffer the system type CR 2032
clock in the case of a
power failure)

USB interface (device) Type USB 1.1

USB-B (Device), PC
Baud rate Adjustable:
1200, 2400, 4800, 9600, 19200 Baud
Data bits 8
Stop bits 2

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Technical data ProLab 2500

Parity None
Handshake RTS/CTS
Cable length Max. 3 m

USB interface (host) Type USB 2.0

USB-A (host), USB device

Guidelines EMC EC directive 2014/30/EU

and norms used EN 61326-1
EN 61000-3-2
EN 61000-3-3
FCC Class A
Meter safety EC directive 2014/35/EU
EN 61010-1
UL 61010-1
CAN/CSA-C22.2#61010-1
IP protection class EN 60529

FCC Class A Equipment Statement

Note: This equipment has been tested and found to comply with
the limits for a Class A digital device, pursuant to Part 15 of the
FCC Rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is
operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if
not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause
harmful interference in which case the user will be required to
correct the interference at his own expense.
Changes or modifications not expressly approved by the
manufacturer could void the user‘s authority to operate the
equipment.

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ProLab 2500 Technical data

Keypad (antibacterial)

Client:Autotype International Limited

Job Ref: 04I0712
Grove Road
Sample Ref No.:LSN 25/71815
Wantage
Oxon Date Received: 15/07/2004
OX12 7B2 Date Reported: 03/03/2005
United Kingdom

CERTIFICATE OF ANALYSIS
AUTOTEX AM

Meth. Desc FILM TEST Supplier: AUTOTYPE

xTest Result Unit Est

x

Salmonella enteritidis 99.6 % Reduction After 24 Hours x

x
Klebsiella pneumoniae 99.4 % Reduction After 24 Hours x
x
Pseudomonas aeruginosa 99.1 % Reduction After 24 Hours x
x
Streptococcus faecalis 99.4 % Reduction After 24 Hours x
x
Phoma violacea 99.0 % Reduction After 48 Hours x
x
Penicillium purpurogenum 99.3 % Reduction After 48 Hours x
x
Bacillus cereus 99.3 % Reduction After 24 Hours x
x
Saccharmyces cerevisiae 99.3 % Reduction After 24 Hours x
x

Comment: The microbiological results demonstrate that the material under test exhibits biocidal activity.

R.P.Elliott C.Fuller J.Lloyd P.M.Sutton J.Elliott J. Francis N.Stanton

CChem, MRSC, MIFST BSc. (Hons.), CBiol., MBiol., BSc. (Hons.) CChem., MRSC. BSc. (Hons.), CBiol., MBiol BSc. (Hons.) BSc. (Hons.)
MIFST
Deputy Managing Company Microbiologist Principal Nutritional Services Senior Senior Microbiologist Senior
Microbiologist Manager Microbiologist Microbiologist
Director

Law Laboratories Ltd Shady Lane, Great Barr, Birmingham B44 9ET England
04I0712/6/1/1

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Technical data ProLab 2500

Client:Autotype International Limited

Job Ref: 05B1760
Grove Road
Sample Ref No.: LSN 26/38123
Wantage
Oxon Date Received: 24/10/2004
OX12 7B2 Date Reported: 21/02/2005
United Kingdom

CERTIFICATE OF ANALYSIS
AUTOTEX AM AGED 15 YEARS

Meth. Desc Harmonised JIS Z2801/AATCC 100

xTest Result Unit Est

x
x

Staphylococcus aureus 99.0 % Reduction After 24 Hours x

x
x
Escherichia coli 0157 99.8 % Reduction After 24 Hours x
x
x
Aspergillus niger 99.1 % Reduction After 48 Hours x
x
x

Comment: The microbiological results demonstrate that the material under test exhibits biocidal activity against
the above listed microbial strains.

R.P.Elliott C.Fuller J.Lloyd P.M.Sutton J.Elliott J. Francis N.Stanton

CChem, MRSC, MIFST BSc. (Hons.), CBiol., MBiol., BSc. (Hons.) CChem., MRSC. BSc. (Hons.), CBiol., MBiol BSc. (Hons.) BSc. (Hons.)
MIFST
Deputy Managing Company Microbiologist Principal Nutritional Services Senior Senior Microbiologist Senior
Microbiologist Manager Microbiologist Microbiologist
Director

Law Laboratories Ltd Shady Lane, Great Barr, Birmingham B44 9ET England
05B1760/1/3/3

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ProLab 2500 Firmware update

16 Firmware update
16.1 Firmware update for the meter ProLab 2500
You can find available firmware update files for your meter on the Internet. With
the "Firmware Update " program and a PC you can update the firmware of the
ProLab 2500 to the newest version.
For the update you have to connect the meter to a PC.
For the update via the USB-B interface, the following is required:
 a free USB interface (virtual COM port) on the PC
 the driver for the USB interface (on the enclosed CD-ROM)
 the USB cable (included in the scope of delivery of the ProLab 2500).

1. Install the downloaded firmware update on a PC.

An update folder is created in the Windows start menu.
If an update folder already exists for the meter (or meter type), the new
data are displayed there.
2. In the windows start menu, open the update folder and start the firm-
ware update program for the meter
3. Using the USB interface cable, connect the ProLab 2500 to a USB
interface (virtual COM port) of the PC.
4. Switch on the ProLab 2500.
5. In the firmware update program, start the update process with OK.
6. Follow the instructions of the firmware update program.
During the programming process, a corresponding message and a
progress bar (in %) are displayed.
The programming process takes up to 20 minutes. A terminatory mes-
sage is displayed after a successful programming process. The firm-
ware update is completed.
7. Disconnect the ProLab 2500 from the PC.
The ProLab 2500 is ready for operation again.

After switching the meter off and on you can check whether the meter has taken
over the new software version (see YOU WANT TO KNOW WHICH SOFTWARE
VERSION IS IN THE METER OR IDS SENSOR, PAGE 113).

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Firmware update ProLab 2500

16.2 Firmware-Update for IDS Sensors

With the "Firmware Update" program and a PC you can update the firmware of
an IDS sensor to the newest version.
You can find available firmware update files for your IDS sensor on the Internet.
For updating, connect the IDS sensor to the ProLab 2500, and the
ProLab 2500 to a PC.
For the update via the USB-B interface, the following is required:
 a free USB interface (virtual COM port) on the PC
 the driver for the USB interface (on the enclosed CD-ROM)
 the USB cable (included in the scope of delivery of the ProLab 2500).

1. Install the downloaded firmware update on a PC.

An update folder is created in the Windows start menu.
If an update folder already exists for the sensor (or sensor type), the
new data are displayed there.
2. In the windows start menu, open the update folder and start the firm-
ware update program for the IDS sensor
3. Connect the IDS sensor to the ProLab 2500 meter.
Only the sensor connection in the lower section of the socket field
(channel 1) is suitable for the firmware update.
4. Using the USB interface cable, connect the ProLab 2500 to a USB
interface (virtual COM port) of the PC.
5. Switch on the ProLab 2500.
6. In the firmware update program, start the update process with OK.
7. Follow the instructions of the firmware update program.
During the programming process, a corresponding message and a
progress bar (in %) are displayed.
The programming process takes up to 5 minutes. A terminatory mes-
sage is displayed after a successful programming process. The firm-
ware update is completed.
8. Disconnect the ProLab 2500 from the PC.
Meter and sensor are ready for operation again.

After switching the meter off and on you can check whether the sensor has
taken over the new software version (see YOU WANT TO KNOW WHICH SOFTWARE
VERSION IS IN THE METER OR IDS SENSOR, PAGE 113).

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17 Glossary
pH/ORP/ISE

Asymmetry see zero point

Electromotive force of The electromotive force U of the combination electrode is the measur-
a combination able electromotive force of an electrode in a solution. It equals the sum
electrode of all the galvanic voltages of the combination electrode. Its depen-
dency on the pH results in the electrode function, which is characterized
by the parameters, slope and zero point.
Junction The junction is a porous body in the housing wall of reference elec-
trodes or electrolyte bridges. It arranges the electrical contact between
two solutions and makes the electrolyte exchange more difficult. The
expression, junction, is also used for ground or junction-less transitions.
ORP voltage (U) The ORP is caused by oxidizing or reducing substances dissolved in
water if these substances become effective on an electrode surface (e.
g. a gold or platinum surface).
pH value The pH value is a measure of the acidic or basic effect of an aqueous
solution. It corresponds to the negative decadic logarithm of the molal
hydrogen ions activity divided by the unit of the molality. The practical
pH value is the value of a pH measurement.
Potentiometry Name of a measuring technique. The signal (depending on the mea-
sured parameter) of the electrode is the electrical potential. The electri-
cal current remains constant.
Slope The slope of a linear calibration function.
Zero point The zero point of a pH combination electrode is the pH value at which
the electromotive force of the pH combination electrode at a specified
temperature is zero. Normally, this is at 25 °C.

Conductivity

Cell constant (C) Characteristic quantity of a conductivity measuring cell, depending on

the geometry.
Conductivity (ϰ) Short form of the expression, specific electrical conductivity.
It corresponds to the reciprocal value of the resistivity.
It is a measured value of the ability of a substance to conduct an electric
current. In water analysis, the electrical conductivity is a dimension for
the ionized substances in a solution.
Reference Fixed temperature value to compare temperature-dependent measured
temperature values. For conductivity measurements, the measured value is con-
verted to a conductivity value at a reference temperature of 20 °C or 25
°C.
Resistance (ρ) Short name for the electrolytic resistivity. It corresponds to the recipro-
cal value of the electrical conductivity.

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Glossary ProLab 2500

Salinity The absolute salinity SA of seawater corresponds to the relationship of

the mass of dissolved salts to the mass of the solution (in g/Kg). In prac-
tice, this dimension cannot be measured directly. Therefore, the practi-
cal salinity according to IOT is used for oceanographic monitoring. It is
determined by measuring the electrical conductivity.
Salt content General designation for the quantity of salt dissolved in water.
Temperature Value of the slope α of a linear temperature function.
coefficient 1
=
TRef Meas * 1 + a * (T - TRef )

Temperature Name of a function that considers the temperature influence on the

compensation measurement and converts it accordingly. Depending on the measured
parameter to be determined, the temperature compensation functions
in different ways. For conductimetric measurements, the measured
value is converted to a defined reference temperature. For potentiomet-
ric measurements, the slope value is adjusted to the temperature of the
test sample but the measured value is not converted.

Dissolved oxygen

D.O. % saturation Short name for the relative D.O. saturation.

Salinity The absolute salinity SA of seawater corresponds to the relationship
of the mass of dissolved salts to the mass of the solution (in g/Kg). In
practice, this dimension cannot be measured directly. Therefore, the
practical salinity according to IOT is used for oceanographic monitor-
ing. It is determined by measuring the electrical conductivity.
Salt content General designation for the quantity of salt dissolved in water.

General information

Adjusting To manipulate a measuring system so that the relevant value (e. g. the
displayed value) differs as little as possible from the correct value or a
value that is regarded as correct, or that the difference remains within
the tolerance.
AutoRange Name of the automatic selection of the measuring range.
Calibration Comparing the value from a measuring system (e. g. the displayed
value) to the correct value or a value that is regarded as correct. Often,
this expression is also used when the measuring system is adjusted at
the same time (see adjusting).
Measured parameter The measured parameter is the physical dimension determined by
measuring, e. g. pH, conductivity or D.O. concentration.
Measured value The measured value is the special value of a measured parameter to be
determined. It is given as a combination of the numerical value and unit
(e. g. 3 m; 0.5 s; 5.2 A; 373.15 K).

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ProLab 2500 Glossary

Molality Molality is the quantity (in Mol) of a dissolved substance in 1000 g sol-
vent.
Reset Restoring the original condition of all settings of a measuring system.
Resolution Smallest difference between two measured values that can be dis-
played by a meter.
Stability control Function to control the measured value stability.
(AutoRead )
Standard solution The standard solution is a solution where the measured value is known
by definition. It is used to calibrate a measuring system.
Temperature function Name of a mathematical function expressing the temperature behavior
of a test sample, a sensor or part of a sensor.
Test sample Designation of the test sample ready to be measured. Normally, a test
sample is made by processing the original sample. The test sample and
original sample are identical if the test sample was not processed.

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124 ba77147e01 07/2016

ProLab 2500 Index

18 Index

A K
Air calibration beaker . . . . . . . . . . . . . . . . . 71 Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
AutoRead . . . . . . . . . . . . . . . . . . . . . . . 69, 74
ORP . . . . . . . . . . . . . . . . . . . . . . . . 43, 45 M
pH . . . . . . . . . . . . . . . . . . . . . . . . . 27, 48 Measured value display . . . . . . . . . . . . . . 21
Measurement accuracy . . . . . . . . . . . . . . . 84
B Measurement data storage
Battery compartment . . . . . . . . . . . . . . . . 107 Edit . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Blank value addition . . . . . . . . . . . . . . . . . . 66 Erase . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Storage locations . . . . . . . . . . . . . . . 101
C Measurement dataset . . . . . . . . . . . . . . . 100
Calibration Measuring
Conductivity . . . . . . . . . . . . . . . . . . . . . 77 Conductivity . . . . . . . . . . . . . . . . . . . . . 74
ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
pH . . . . . . . . . . . . . . . . . . . . . . . . . 29, 46 O2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Calibration evaluation ORP . . . . . . . . . . . . . . . . . . . . . . . 43, 44
Conductivity . . . . . . . . . . . . . . . . . . . . . 79 pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Measuring method . . . . . . . . . . . . . . . . . . 56
O2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 Blank value addition . . . . . . . . . . . . . . 66
pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Sample addition . . . . . . . . . . . . . . . . . 62
Calibration interval Sample subtraction . . . . . . . . . . . . . . . 64
Conductivity . . . . . . . . . . . . . . . . . . . . . 88 Standard addition . . . . . . . . . . . . . . . . 57
pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 Standard subtraction . . . . . . . . . . . . . . 59
Calibration points Menu for calibration and measurement settings
pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 pH/ORP . . . . . . . . . . . . . . . . . . . . . . . . 85
Calibration records . . . . . . . . . . . . . . . . . . . 78 Menus (navigation) . . . . . . . . . . . . . . . . . . 21
Cell constant . . . . . . . . . . . . . . . . . . . . . . . 77 Messages . . . . . . . . . . . . . . . . . . . . . . . . . 23
Comparison measurement (D.O.) . . . . . . . 71
Connect the power pack . . . . . . . . . . . . . . 13 P
Connecting a PC . . . . . . . . . . . . . . . 102, 103 pH buffer sets . . . . . . . . . . . . . . . . . . . . . . 82

D R
Dataset . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Date and time . . . . . . . . . . . . . . . . . . . . . . . 25 RS232 interface . . . . . . . . . . . . . . . . . . . 103
Default settings
Measured parameter . . . . . . . . . . . . . . 93 S
System settings . . . . . . . . . . . . . . . . . . 95 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Sample addition . . . . . . . . . . . . . . . . . . . . 62
Sample subtraction . . . . . . . . . . . . . . . . . . 64
F Scope of delivery . . . . . . . . . . . . . . . . . . . . 12
Firmware update . . . . . . . . . . . . . . . . . . . 119 Single-point calibration
pH . . . . . . . . . . . . . . . . . . . . . . . . . 30, 34
I Slope
Initial commissioning . . . . . . . . . . . . . . . . . 12 ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Initialize . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Instrument connectors . . . . . . . . . . . . . . . . 16 Stability control
Interval for calibration . . . . . . . . . . . . . . . . . 84 Automatic . . . . . . . . . . . . . . . . . . . . . . 92
Manual . . . . . . . . . . . . . . . . . . 27, 43, 69
Standard addition . . . . . . . . . . . . . . . . . . . 57

ba77147e01 07/2016 125

Index ProLab 2500

Standard addition with blank

value correction . . . . . . . . . . . . . . . .66
Standard subtraction . . . . . . . . . . . . . . . . .59
Storage interval . . . . . . . . . . . . . . . . . . . . .96
Storing in memory . . . . . . . . . . . . . . . . . . .96
Automatic . . . . . . . . . . . . . . . . . . . . . . .96
Manual . . . . . . . . . . . . . . . . . . . . . . . . .96

T
Temperature compensation . . . . . . . . . . . .76
Temperature measurement
Conductivity . . . . . . . . . . . . . . . . . . . . .76
ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
O2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
pH . . . . . . . . . . . . . . . . . . . . . . . . . .28, 46
Three-point calibration
ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
pH . . . . . . . . . . . . . . . . . . . . . . . . . .31, 35
Transmitting data . . . . . . . . . . . . . . . . . . .102
Automatic . . . . . . . . . . . . . . . . . . . . . .104
Manual . . . . . . . . . . . . . . . . . . . . . . . .104
Transmitting measured values . . . . . . . . .102
Two-point calibration
ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
pH . . . . . . . . . . . . . . . . . . . . . . . . . .30, 34

Z
Zero point of pH electrode . . . . . . . . . . . . .29

126 ba77147e01 07/2016

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