MASTERING NGS

AND
ADVANCED ANALYSIS
INDUSTRIAL BIOINFORMATICS
4-MONTH
INTERNSHIP
Your NGS Internship journey starts here
At Dr.Omics Labs, we're excited to offer you an opportunity to
embark on an incredible journey of discovery and innovation in the
field of Next-Generation Sequencing (NGS).

About Us
Dr.Omics Labs is a leader in the field
of bioinformatics and genomics
research. We are committed to
pushing the boundaries of scientific
knowledge and fostering the next
generation of bioinformatics experts.

The Internship Experience

Our Industrial Bioinformatics Long-term Internship program is
designed to provide you with a comprehensive understanding of
NGS technologies, data analysis, and their real-world
applications.

Four Months Internship Program Highlights

Hands-on experience with Next-Generation Sequencing
(NGS).
Expert-led workshops and training.
Access to cutting-edge bioinformatics tools.
Networking opportunities.
Coursework Overview
(Modules)
1. Basics of Bioinformatics & NGS

2. Linux, & Cloud Computing

3. Python, Biopython and its Application in NGS

Data Analysis Techniques

4. R and Introduction to Bioconductor

5. RNA Sequencing (Reference and DeNovo

Based)

DNA Sequencing (Variant calling),

Annotation
6. OR
Targeted Metagenomics Data analysis

7. Research Project based on NGS (1 month)

Module 1: Basics of
Binformatics & NGS

1.1) Basics Bioinformatics

Defining & Understanding Bioinformatics
Introduction to genomic bioinformatics
Learning Databases (GenBank, PubMed,
KEGG, Clinvar, UCSC, Uniprot, PDB )
Understanding Bioinformatics Tools (BLAST,
Stand-alone BLAST, Mega)
Introduction to the Linux system
Bridge the gap between biotechnology and
bioinformatics

1.2) Next Generation Sequencing

Role of Bioinformatics in NGS
Introduction to Next-Generation
Sequencing
Introduction to types of NGS techniques
NGS Applications
NGS Platforms
Understanding Data analysis techniques
using NGS
Scope of NGS
Module 2: LINUX & Cloud
Computing

Linux overview and significance

File and directory operations (create, copy,
move, delete)
Text file editing and creation
Process management (introduction and
termination)
Basic networking and ownership overview
Conclusion and further resources
Basics of Cloud technology (AWS)
Basics of Pipeline Engineering
Module 3: Python, Biopython and
its Application in NGS Data
Analysis Techniques
3.1) Python Programming
Introduction to Python language
Role of Programming in Bioinformatics
Installation of Python on various platforms
Installation of IDE
Print function
Comments
User input
Command line arguments
Data types
Variables and rules to create a variable
In-built functions of python
Slicing and indexing in String
String and data formatting
Control statements (if -else, If -elif-else, for
loop, etc)
Python data structure (List, Set, Tuple, etc)
Methods of data structures
Function introduction & its requirement
Exception Handling, File Handling & Pandas
Library
3.2) Biopython
Introduction to Biopython
Installation of Biopython
Conversion of a string into a biological
sequence
Obtaining complement, reverse complement,
transcribe, reverse transcribe, and translation
from a sequence
Finding GC content from a sequence
SeqIO object:
Reading various biological file formats such
as Fasta and GenBank
Analysis of fasta sequences
Finding GC content of a fasta file containing
multiple sequences and storing the data in a
file
Converting a GenBank file into fasta format
Accessing NCBI’s Entrez databases: Entrez
Guidelines (EInfo, ESearch, etc)
Module 4: R and Introduction to
Bioconductor
4.1) R Programming
Introduction to the R language
Importance of R in Bioinformatics
Installation of R
Installation of IDE (R studio)
Print, cut, and paste functions
Comments
Variables
Data types
Functions of math
Operators
Installation of packages
String formatting
Learning Control Statements (if -else, while
loop, break, etc.)
R Data Structures (Lists, Vectors, Arrays, etc)
File Handling & User-Defined Functions
4.2) Introduction to Bioconductor
Bioconductor package installation
Sequence analysis
Basics of seqinr package
Import and export FASTA sequences
Reverse complement
GC content
Retrieving genbank and fasta files from NCBI
Statistical study for Analysis (z-test, t-test,
etc)
Plot generation for data visualization (box
plot, PCA plot, Heatmap, Volcano Plot)
Module 5: RNA Sequencing
(Reference and DeNovo based)
5.1 Reference based
Introduction to RNA Seq
Necessary Tools installation
Learn how Data Retrieval is done
Quality Check of reads using FASTQC, FastP
Trimming and cleaning of data using Cutadapt
Understanding mapping of reads on reference
genome and file formats (SAM, BAM) with
Hisat2
Visualization techniques
Gene Expression Quantification & Analyzation
Pathway & Gene ontology enrichment analysis
using StringTie, DESeq2
Pathway Network analysis using KEGG

5.2 DeNovo based

Generation of transcriptomic assembly
Statistical study of assembly
Mapping and abundance calculation
Visualization of mapped reads using
Cytoscape
Generate the count matrices for differential
expression analysis
Module 6: DNA Seq (Variant
calling), Annotation
Introduction and installation of tools
Data retrieval & quality check of reads
Mapping of reads using reference Genome
Understanding Mapping Output
Variant detection
Visualization of variation
Annotation and variant effect prediction
Determining effect of coding non-synonymous
mutation on protein function ability

Module 7: Targeted Metagenome

analysis
Data Downloading (NCBI SRA/EBI SRA)
Quality control using Fastqc
Trimming (cutadapt/Fastp/Trimmomatic)
Demultiplexing
Data Importing
Quality Check by DADA2
Phylogenetic Diversity Analysis (Alpha and
Beta Diversity)
Taxonomy Analysis
Krona Plot
Phylogenetic Tree Construction using MEGA
 Pro g r a m S t r u c t u r e
Duration: 4 months
Coursework: 3 months
Project: 1 month

From Theory to Practice:

Bridging the Gap in Genomic Research

Comprehensive NGS Training: Understand NGS fundamentals,

data generation, and quality control.
Bioinformatics Tools and Resources: Master NGS analysis tools
and explore essential genomic databases.
Real-world Projects: Engage in genome assembly, variant calling,
and functional annotation projects.
Professional Development: Enhance your resume and gain
mentorship from experienced professionals
Frequently Asked Questions
Q: Are these courses suitable for those new to the field
without prior experience?
A: Yes, our courses are designed to cater to beginners with no prior
experience in the field. We provide foundational content suitable
for all skill levels.

Q: Will I receive a certification upon completing the course?

A: Absolutely, a digital certificate will be awarded upon course
completion. You'll receive this certificate via email.

Q: Do the courses include practical projects and research

opportunities?
A: Certainly, our courses incorporate practical projects and
research opportunities to ensure hands-on learning and the
practical application of acquired knowledge.

Q: Can I access class recordings if I miss a class?

A: Yes, class recordings are available. We'll send you the recording
link via email if you miss a class, typically on the day following the
live session.

Q: Can I continue to access course materials and resources

after finishing the course?
A: Absolutely, you'll retain access to course materials and resources
even after completing the course. These materials will be shared
with you via email or WhatsApp.
Terms and Conditions

Maintaining Discipline during the Tenure.

It is mandatory to maintain 85% attendance for all students.
Students must maintain an average ‘A2’ grade throughout
their training period.
Project completion is a must for research.
Publication Students must participate actively in the Project
group
 NEED MORE INSIGHT & SUPPORT?

CONTACT US!

602/E, W NO 3, G/F, L/SIDE, SEQNO-M, H 3/727

GADAIPUR, NEW DELHI , SOUTH-WEST DELHI-110030

+91 9310870544, 7888118030, 7843088966

INFO@DROMICSLABS.COM

WWW.DROMICSLABS.COM

Thank you!

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