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Chapter 1 Automation System Overview

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0% found this document useful (0 votes)
85 views19 pages

Chapter 1 Automation System Overview

Uploaded by

Yazid Mahi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Chapter 1:

DS2® Automated ELISA System Overview

Sample to Insight
DS2® Automated ELISA System Overview

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DS2® configuration
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DS2® Automated ELISA System: characteristics
00
DS2 Automated ELISA System: overview
00
DS2® Automated ELISA System /Components
00
DS2® Automated ELISA System /QFT-Plus

DYNEX consumables 00

00

00

00

00

Sample to Insight
Chapter 1: DS2® Automated ELISA System Overview
DS2® configuration

❑ Cat # 9002251:
→Dynex DS2 Automated ELISA Instrument: full configuration with PC, printer and UPS.
❑ Cat # 9002724:
→Dynex DS2 Automated ELISA Instrument: only DS2 machine
❑ Cat # 9025521:
→Dynex DS2 Multitube license (dongle)

Sample to Insight
DS2® Automated ELISA System: characteristics

❑ Full automated:
→Sample distribution
→Reagent addition
→Incubation
→Plate washing
→Signal detection
→Result calculation

❑ 2-plate ELISA processor; can run 2 plates simultaneously


❑ Up to 12 different assays per run.
❑ 100 samples per load (5 X 20); allows continuous loading.
❑ USB connection

Sample to Insight
DS2® Automated ELISA System: characteristics

❑ Dimensions: Width: 54 cm/ Depth: 68 cm/Height: 66 cm


❑ Weight (net): 48 kg
❑ Voltage: 100-240V auto-switching/Frequency: 50/60Hz/Power
consumption: < 300 VA.
❑ Reader Specifications: Dynamic range: -0.100-3.0 OD/ Spectral
range: 405-690 nm
❑ Filter slots: 6

❑ Temperature range: Ambient + 4°C to 40°C/shaker


❑ Liquid level sensing by pressure tip detection, clot detection.
❑ Supports: Codabar, Code 39, Standard 2 of 5, IATA, UPCE and
UPCA.

Sample to Insight
DS2® Automated ELISA System: overview

Instrument components – overview System startup and shutdown

Starting the DS2 system


1. Power on the DS2 instrument.

2. Power ON the computer, monitor, and printer.

3. Run the “Start of Day” protocol.


2

Switch off the DS2 system


1
1. Run the “End of Day” protocol.

2. Turn off the computer, monitor, and printer.

3. Turn off the DS2 instrument.

1 DS2 Automated ELISA System

2 PC work station

Sample to Insight
Chapter 1: DS2® Automated ELISA System Overview
Components of DS2® Automated ELISA System

Instrument components – operational view A


5
1 Wash head
2 Barcode scanner 7
6 4
3 Sliding cover
4 Incubator door lever
5 Pipetting/washing area 1 3
9 8
6 Reading optics
2
7 Incubation chamber
8 Reagent block
10
9 Sample rack
Instrument front
10 Access panel to buffers, waste bottles, tip waste
11 Container C connection tubing 11

12 Wash head cleaning fluid container C

12

Instrument back
Sample to Insight
Chapter 1: DS2® Automated ELISA System Overview
Components of DS2® Automated ELISA System

Instrument components – operational view B

Pipetting module

Sample tips rack (x2)


Deep well dilution strips (x12)

Embedded reader and 2


plate incubator)
Reagents racks (x2)
Independent plate carriers (x2)

Samples Rack (x5)


Barcode reader

Wash buffer container A Liquid waste container

Wash water container B


Waste tip container
Sample to Insight
Chapter 1: DS2® Automated ELISA System Overview
Components of DS2® Automated ELISA System

Pipetting module

Sample tips rack (x2)

Deep well dilution strips (x12)

Embedded reader and 2 plate


incubator)
Reagents racks (x2)

Independent plate carriers (x2)


Samples Rack (x5)

Barcode reader

Wash buffer container A


Liquid Waste
Wash water container B container

Waste tip container


Sample to Insight
Components of DS2® Automated ELISA System

SAMPLE RACK

REAGENTS BLOCK

Sample to Insight
DS2® Automated ELISA System : pipetting module

Sample to Insight
DS2® Automated ELISA System : washing module

Sample to Insight
DS2® Automated ELISA System : waste container

Sample to Insight
DS2® Automated ELISA System /Components

❑ Delivered with:
→5 sample racks for 100 samples (additional need to be ordered from Dynex).
→2 reagent racks
→8 ways wash head assembly with purge tray.
→2 wash buffer containers
→1 wash bottle
→1 tip waste container
→4 filters: 405,450,490 and 620 nm.
→1 HP Desktop with Monitor
→1 HP printer
→Softwares (DSMatrix, LIS-link, Toolbox)
→Dongle
→Power cord, USB cable
→Calibration and cleaning tools
→Documents (warranty, DOC, UM, checklist)
→Sample tips (4X108)/reagents tips (1X108)
Sample to Insight
DS2® Automated ELISA System /QFT-Plus

❑ QFT® - Plus requires use of:


→DSMatrix SW version 1.34.5
→LIS-Link SW version 2.0.3 (If LIMS integration is required)
→DS2 QFT-Plus V1.6 with manual standard dilution
→DS2 QFT-Plus V2.6 with on-board standard dilution
→Dongle
→Boolean function

❑ QFT® -TB 3-tubes requires use of:


→DS2 QFT-Plus V3.6 with manual standard dilution
→DS2 QFT-Plus V4.6 with on-board standard dilution
→Dongle
→Boolean function

Sample to Insight
DS2® Automated ELISA System Consumables

❑ Cat # 9022717: QIAGEN DS2 Installation Consumables kit

Catalogue number Consummables Quantity

9022718 DYNEX Blue Sample Tips 6

9022719 DYNEX White Reagents Tips 1

9022720 DYNEX Reagent tubes Lg (25 ml) (10 pieces/Pack) 2

9022721 DYNEX Reagent Tubes Sm (15 ml) (10 Pieces/Pack) 6

9022722 DYNEX Control Vials (33 Pieces/Package) 10 Note: All consumables


can be ordered
9025594 DYNEX Deep Well Strips (250x8-well) 1 separately under the
mentioned catalog
❑ Cat # 9023805: DYNEX DS2 Regular Consumables kit
numbers.
Catalogue number Consummables Quantity

9022718 DYNEX Blue Sample Tips 6

9022720 DYNEX Reagent tubes Lg (25 ml) (10 pieces/Pack) 2

9022721 DYNEX Reagent Tubes Sm (15 ml) (10 Pieces/Pack) 6

9022722 DYNEX Control Vials (33 Pieces/Package) 10

Sample to Insight
DS2® Automated ELISA System Consumables (for the lab routine)

Blue Sample Tips ref 9022718 Deep Well Strips ref 9025594

White Reagents Tips ref 9022719

Control Vials ref 9022722 Reagent Tubes Small ref 9022721 Reagent Tubes Large ref 9022720

Sample to Insight
DS2® Automated ELISA System -barcodes

Sample to Insight
Thank you for your attention.

Questions?

Trademarks: QIAGEN®, Sample to Insight®, QuantiFERON®, QFT® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as
such, are not to be considered unprotected by law. PROM-15006-001 © 2019, QIAGEN, all rights reserved.

Sample to Insight

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