iFlash 1800

Chemiluminescence Immunoassay Analyzer

Service Training 2021
 1 Mechanical System
- Bone & Muscle

Contents 2 Hydraulic System

- Blood System

3 Hardware System
- Brain & Nervous System
 Specifications
3
Detection principle: Acridinium Ester/ Magnetic Beads

Aspirate Volume: 5~150 uL

Weight & Size: 150kg, 1030×750×710mm (L,D,H)

Throughput: Up to 180 T/H

Reagent position: 20 refrigerated reagents onboard

Sample position: 50 sample positions with STAT

Incubation carousel: 75 incubation positions

RV feeder: up to 1000 reaction vessels, can load anytime

 Analyzer Structure
4

Consumable Upper lid (Shading cover)

Indicators

Network port
Sample loading area

Power port & switch Sample loading indicator

Pre Trigger/ Trigger

solution loading area

Solid waste container

Running indicator
Analyzer Structure
5
 Analyzer Structure
6

Probe
Gripper

Tube & Floater

sensor connector

Dust gauze
 Analyzer Structure
7
Magnetic
Separation Probe
Component

RV feeder

RV / Magnetic Incubation carousel

separation carousel

Reading Station Reagent carousel

Inner wash buffer tank (2L)

Vessel
Discarding
Mixer Probe washing station
Reagent carousel button
Sample aspiration position
Analyzer Structure
8

①RV Feeder
②Magnetic Separation Component
③Mixer
④Gripper
⑤Incubation Carousel
⑥Sample/Reagent Probe
⑦Reagent Carousel
⑧Reading Station
⑨Sample Loading Area
Dead Volume
9
 Test Panel
10
Autoimmunity Reproductive Health Infection Others

Rheumatoid Arthritis Fertility TORCH EBV Thyroid Cardiac Markers

Anti-CCP AMH Toxo IgG EB VCA IgG TSH CK-MB
RF IgG Inhibin B Toxo IgM EB VCA IgM Anti-TPO Myoglobin
RF IgM Inhibin A CMV IgG EB VCA IgA Anti-TSHR Troponin-I
RF LH CMV IgM EB EA IgM Tg BNP
RA33 IgG FSH HSV-1 IgG EB NA IgG Anti-Tg Bone Metabolism
Connective Tissue Disease HCG HSV-2 IgG EB NA IgA FT3 25-OH Vitamin D
ANA Prolactin HSV-1 IgM Respiratory Disease FT4 PTH
dsDNA IgG DHEA-S HSV-2 IgM Mycoplasma pn. IgG T3 Metabolism
Sm IgG Unconjugated Estriol Rubella IgG Mycoplasma pn. IgM T4 Insulin
SS-A IgG E2 Rubella IgM Chlamydia pn. IgG Tumor Markers C-Peptide
SS-B IgG PAPP-A Chlamydia pn. IgM PG I Cortisol
Scl-70 IgG free βHCG Blood Bank PG II ACTH
Jo-1 IgG Progesterone Anti-TP (Syphilis) AFP Adiponectin
RNP70 IgG Testosterone Anti-HCV * CEA Liver Fibrosis
Autoimmune Liver Anti-Phospholipid Syndrome HBsAg * CA 125 HA
SMA IgG Cardiolipin IgG Anti-HBs * CA 15-3 PⅢPN-P
AMA-M2 Cardiolipin IgM HBeAg * CA 19-9 Col Ⅳ
Vasculitis Diagnostics
PR3 IgG
Cardiolipin IgA
Anti-Cardiolipin
Anti-HBe *
Anti-HBc *
Free PSA
Total PSA
Laminin
Over
GBM IgG β2-Glycoprotein I IgG HIV Combo * CYFRA 21-1
MPO IgG
Autoimmune Diabetes
β2-Glycoprotein I IgA
β2-Glycoprotein I IgM
Inflammation
PCT
NSE
Anemia
120 Test
IA-2A
IAA
Anti-β2-Glycoprotein I
Novel Coronavirus
Ferritin
Folate Panels!
GADA iFlash-2019-nCoV IgM Vitamin B12
ICA New iFlash-2019-nCoV IgG
ZnT8A iFlash-2019-nCoV IgA
iFlash-2019-nCoV Ag
iFlash-2019-nCoV NAb
Work Flow of Analyzer
11
12
PART ONE Mechanical System
 Consumables
14
Each test requires 100uL of Pre-trigger solution and 200uL of Trigger solution

Pre-Trigger Solution Trigger Solution Wash Buffer Wash Buffer Reaction

Hydrogen Peroxide Sodium Hydroxide Outer Tank B Inner Tank A Vessels
H2O2 NaOH 10L 2L
 Consumable Indicators
Consumable Status Light
15
Sufficient Green (stable)
Insufficient
Yellow (stable)
Reaction vessel
Empty (failed to
replenish RV after Green and Yellow
couple attempts) (flash alternately)

Sufficient Green (stable)

Pre-trigger / Trigger Insufficient Yellow (stable) (set in sw)

Empty Green and Yellow

(flash alternately)
Sufficient Green (stable)
Insufficient
Wash buffer Yellow (stable)

Empty Green and Yellow

(flash alternately)
Normal Green (stable)
Early warning
Solid waste tank Yellow (stable)
＞ 250
Full Green and Yellow
＞ 350 (flash alternately)
 Sample Racks
16
Sample Rack

Various Sample Rack Type

Rack Type NO. Color Color Code

Routine N001~N100 Grey
STAT E001~E010 Red
Calibration S001~S010 Yellow
QC C001~C010 Blue
Sample Loading Area Indicators
17

Light Status

Off No Sample loaded or power off

Rack has been loaded but not analyzed yet, can be taken
Green （ stable ） away.

Yellow (stable) Samples are being aspirated, cannot load new rack on this
No rack on position position.

Yellow (stable) Samples have been aspirated, can be taken away or leave on
With a rack on position position for auto reflect & retest.

Sample tests have been completed, rack can be taken away.

Green (Flash)

Running light (Yellow) Sample tray is moving, cannot load or take out the rack.

Green and Yellow Errors occur when samples are being scanned.
(flash alternately)
 Reagent Kit
18
Reagent System
Reagent Composition

Antigen/antibody-coated paramagnetic
4 Solid phase
microparticles
1 Conjugate Acridinium-ester-labeled antigen/antibody conjugate

Sample dilute Phosphate buffer

Assay dilute Protein stabilizer

2/3
Sample treating agent Surfactant

Neutralizing antigen working solution Contain the antigen used for competitive immunoassay
4 3 2 1

4 ： Transparent reagent bottle with mixing structure at the bottom containing antigen/antibody-coated paramagnetic microparticles
1 ： Black opaque reagent bottle containing acridinium-ester-labeled antigen/antibody conjugate

2/3 ： The two transparent reagent bottles in the middle contain sample dilute/ assay dilute/ sample treating agent/ neutralizing antigen working solution or empty
Reagent Carousel Button
19

Light Status

Off Power off or uninitialized

Green （ stable ） The cover can be opened to load the reagents.

The reagent carousel is working (scan/ mix/ aspiration), the

Yellow (stable)
cover cannot be opened.

Green and Yellow The reagent carousel goes wrong, such as jammed or cover
(flash alternately) open when the carousel is rotating.
Tube & Floater Sensor Connector
20
Waste Liquid Floater

Waste Tank

Wash Buffer Tank

Reagent Carousel
Condensate Water
Discharge outlet

Wash Buffer Floater

 Tube & Floater Sensor Connector
21

Drainage requirement

• The waste liquid from the analyzer could drain directly into the sewer, the waste
tube should be no longer than 5m;
• The end of the discharge tube shall not be immersed in the waste liquid. If use
waste liquid tank, the discharge tube cannot exceed more than 35mm of the
waste liquid tank cap.
• The sewer port cannot be higher than the bearing table;
• All drainage tubes cannot be bent and cannot move upwards.
• The condensation water drain pipe (waste Ⅱ) can only be discharged by waste
liquid tank.
 Tube & Floater Sensor Connector
22

Wash Buffer Outer

Tank 10L
Waste Tank 10L
Auto replenishment
(1) Waste liquid
to the inner tank
throughout the
reaction process
(2) Condensation
water from the
reagent Carousel
RV Feeder
23

• Large vessel loading capacity: Up to

1000 vessels
• Can be added during testing
• Automatic replenishment of reaction
vessels during testing
 RV Feeder
24

Dispense chute Ejector

Vessel slide channel Elevator

Vessel dispenser
 RV Feeder
25

Left view

Sensor

Top view
• Sensor function ：
1. Home position
2. Jam detecting
 RV Feeder
26
 Ejector and dispense chute
Home sensor

Ending sensor
(slow down)

Dispense chute
status sensor
(Open or close)
 RV & MS Carousel
27

 Structure ：
1. Outer circle is RV carousel

2. Inner circle is Magnetic

separation carousel
Inner & outer circle

Reaction vessel
detect sensor
RV & MS Carousel
28
 RV & MS Carousel
29

Pre-trigger

2,4 step dispense  Four times magnetic separation,

achieve the best washing effect
1,3 step Aspirate

1,3 step dispense

 Accurate array magnetic field surround

design ， precisely control magnetic
2,4 step Aspirate
RV dispense
beads loss & cleaning effect on process
Inner circle grip of washing, achieve optimum magnetic
Outer circle grip separation and repeatability
RV & MS Carousel 30

A B
C

 A(1,3 step), B(2,4 step) liquid Dispensing probe tilt

fixed ， fix it after adjustment tool confirm
location
 C is pre-trigger dispensing probe
RV & MS Carousel
31

A
B

 A(1,3 step), B(2,4 step) aspirating probe.

 Mixer
32
 Mixing component Top view
(Mixer)

Motor sensor

Guard sheet

Step motor  No-contact eccentric vortex mixing

 Step motor drive directly, high

speed rotate
Incubation Carousel
33

 Stable temperature: 37°C

accuracy: ±0.3°C
Fluctuate range: ±0.1°C

 30+25+20 ， 75
positions ， all kinds of
reaction mode

 24V Heating Film

 Uses encoder and

optocoupler to precisely
Encoder control the position
Incubation Carousel
34
Reagent Carousel 35

 20 2~8℃ refrigerated reagent position

 Continuous loading during testing
 Automatic scanning to identify kit
information
 Automatic mixing of magnetic particles
 Reagent Carousel 36
Reagent
Barcode
reader

Code disk
（ 20 gear ） Peltier

Home sensor

Jam detect sensor

Reagent Carousel 37

Reagent tray

Reagents mix components

Gear

Reagent pot
(Outer carousel)

Barcode Scanner

Driver component
Reagent Carousel
38
Reagent Carousel 39
Cooling liquid tube

Temperature
sensor cable
Peltier cable (Black)
(Black & Red)
 Reagent Carousel
40

Water cooling
pump

Cooling fan &

cooling fin Coolant
reservoir
 Optical System (Reading station)
41

PMT

Vessel dicarding
Waste liquid probe component
Optical System (Reading station)
42
 Gripper Component
43
 RV carousel--incubation carousel--mixing component

 Incubation carousel--magnetic separation carousel–-Optical system

(Reading station)--Discard reaction vessel

r
pe

 Transport vessel automatically

ip
Gr

 Auto detect vessel and collision

 Auto clear status
Gripper Component
44

Gripper

Horizontal drive component

Driver
component

Vertical drive component

 Gripper Component
45
Guard sheet

Step Motor
Collision detect sensor

Grabhook

Gripper adapter board

Grab Vessel
detect sensor

Guard sheet
Sam/Rea Probe
46

e
ob
Pr
 Fast and accurate sample and

ea
/R
m
reagent aspiration and dispensing

Sa
 Intelligent aspiration and collision
detection
Sam/Rea Probe
47
 Sam/Rea Probe
48
Liquid level
Collision detect Collision detect
detect board
sensor guard sheet

Probe
 Sample Delivery System
49
 Sample delivery & processing continuously

Sample transfer carrier

component Sample cart
component
10 positions, 50 sample positions
3 STAT positions set in software
 Sample Delivery System
50

Sample loading area

Home
sensor & motor

Sample Rack
Detection Board
Code disk &
Collision detect sensor

Sample rack
detect sensor
 Sample Delivery System
51
 Sample cart component
Barcode reader

Sensor Function Control requirement

description
FXC2 1. sample rack back form channel,
Detecting sample Y-axis can be handled accordingly
FXC2 rack on or not 2. After scan last position on
sample rack
Detecting sample
FXC3 cuvette on sample Active after certain position
rack or not

FXC3 Detecting sample rack Not permit sample transfer carrier

FXC4 between mover and move on X-way after active
sample feeding assembly

FXC4
 Sample Delivery System
52

Finger

Z axis sensor

 Finger 3 status ：

 Lower position (Home position)

Z axis motor  Middle position ： Drag sample rack

 High position ： Push sample rack
from sample loading component to
sample tray
PART TWO Hydraulic System
 Hydraulic System
Wash
54
Waste
Condensate
Pre-trigger&
trigger

Sample/ Reagent carousel cooling water

reagent
Pre-trigger
and trigger
solution
Waste liquid
aspiration
Magnetic
separation
 Hydraulic System
Sample/reagent probe washing and sample/Reagent-transfer unit
55
 Reagent/Sample probe aspirating (P7 drive it)
 Dispensing to vessel （ P7 drive
it ）
 Cleaning reagent probe inside
（ membrane pump drive it ）

 Cleaning reagent probe outside

（ membrane pump drive it ）
 Hydraulic System
56
A:Wash buffer transfer unit

B:Magenetic separation wash buffer  Wash buffer inject to vessel(Plunger pump drive it )

 Drain waste liquid for vessel （ Peristaltic pump drive it ）

 Hydraulic System
Pre-trigger and trigger solution transfer unit
57

 Pre-trigger solution inject

（ Rotate plunger pump drive it ）

Heater
 Trigger solution inject （ Plunger
pump drive it ）

 After test finished, drain waste liquid

（ membrane pump drive it ）
 Three-way Solenoid Valve
58
NC
COM
NO

COM

 When the valve is energized, NC

the COM and NC are connected

 When the valve is not

energized, the COM and NO are NO
connected

 It acts as a channel switch

 Plunger Pump
59

P6 P7 P8
Plunger
pump Range Ability Description Difference
Note
P6 is different from
P6 500ul Used for trigger p7,p8.its top part is
solution
opaque
They can not be
P7 500ul Used for replaced each
Sample/reagent other when one is
They look the same,but broken
their range ability is
Used for magenetic different
P8 2500ul
separation
 Rotate Plunger Pump
60

Outlet

Inlet
 Function:It used to transfer the Pre-trigger solution

 Notice:The interface is broken easily

 Membrane Pump
61

P1 P2 P4

Membrane pump Description Voltage Note

P1 Used for sample/reagent probe inside cleaning 24V

P2 Used for sample/reagent probe outside cleaning 24V Apperance and model are different

P4 Used for cleaning solution transfer from tank B to 24V

tank A
 Two-way Vacuum Pump 62

Notice the liquid inlet and outlet

direction

 After test finished ， It is used for draining vessel waste liquid and washing pool to waste bottle
 Peristaltic Pump
63

 Peristaltic pump tube life span is about 6 months

 Used for draining magnetic separation cleaned solution

One-way Valve & Heater & Filter
64

One-way valve Liquid go through only from one direction

Heater Heating trigger and wash buffer

Filter Filter impurities in the wash buffer

 Confluence Pool
65
Waste liquid from magnetic separation

Pre-trigger

Waste liquid

Wash buffer adding

Condensate water

 1. Only 1 confluence pool

 2. Used to wasted liquid dispensing
 3. Wash buffer adding
 Washing Pool
66

 360 ° shower cleaning

 Exterior washing for sample/reagent probe

PART THREE Hardware System
 Hardware System
Heater
68
Temperature collection board 1
Peltier
Temperature driving board
Fan
Temperature collection board 2
Water cooling pump

Carrier driver
board
RJ454 Sample sensor Sample indicator
PC
adapter MD4 board board
Float sensor
Motor （ 6 ）

SM Aspirating probe
Float sensor
clot detection board
MD3 Pump ， Valve
Optocoupler
Incubation
Motor （ 5 ）
carousel encoder

PMT counting
Optocoupler PMT module
board
MD2
Motor （ 5 ） Valve ， indicator

Pump,
Sam/Rea probe clot
valve ， indicator
detection board
Optocoupler MD1 Liquid level detection
board
Reagent barcode
Motor （ 5 ）
scanner
Hardware System
69
 MD Board
70
 Motor driver board is called MD board
 MD1~MD4 use the same PCBA, and the only
difference part is the J15 dial switch ：

 Power port ： J1, input 24V

 Motor port ： J2-J7, total six motors driver port
 Sensor port ： J9-J12, used for optocoupler
sensor detection
 Floater port ： J13, used for floater and
buttons detection
 Encode port ： J14, used for encoder
detection.
 Dial switch ： J15, used for differentiate
between MD1-MD4
 Jumper port ： J16, only MD1 needed
 CAN port ： J17&J18, used for CAN
communication
 LAN port ： J22 ， only MD4 need to be
connect to LAN
 Multifunction port ： J25, used for
communication between PCBA
 Power driving port ： J26, used for driving
pump 、 valve and indicator
 Maintenance port ： core board J3, used for
maintenance
 TF card port ： used for burning core board
 MD Board
71
Board indicator ：

 D39 ： power indicator of core

board ， blue, normally on
 D40 ： status indicator of core board,
yellow flash
 D35 ： 24V power indicator ， green,
normally on
 D33 ： 5V power indicator, green, normally
on
 D34 ： 3.3V power indicator, green,
normally on
 D36 ： 2.5V power indicator, green ,
normally on
 D37 ： 1.2V power indicator, green,
normally on
 D14 ： FPGA configuration indicator light,
orange/yellow, normally on
 D15 ： FPGA status indicator 1 ， flash after
FPGA work
 D16 ： FPGA status indicator 1 , only MD1
has breathing flash
 MD Board
72

MD1-MD4 differentiate ：

 Differentiate by dial switch :

 Binary system
 MD1: 0001 (4321)
 MD2: 0010 (4321)
 MD3: 0011 (4321)
 MD4: 0100 (4321)
 Power System
73

 Power system
 The power system is divided into two 24V power supply modules: 24V450W power supply
module, 24V600W power supply module
 24V450W power supply module, mainly used for drive motor, pump and valve, float
detection and supply for MD1-MD4 board
 24V600W power supply module, mainly used for peltier and heater power supply
 Power Module
74
DC power
transfer board

RJ45
adapter

24V450W power
Power
module
switch

24V600W power
Power module
filter
 LAN Transfer Board
75
RJ45 adapter

 This board is the connector between PC and MD4

Carrier Driver Board
76

Port introduce ：
 J3 ： Z axis motor port
 J4 ： Y axis motor port
 J5 ： Power and communication port
 J6 ： Optocoupler port 1
 J7 ： Optocoupler port 2 （ reserved ）
 J8 ： Optocoupler port 3
 J2 ： Reagent barcode scanner port
 J10 ： Maintenance port
 J12 ： CAN port （ reserved ）

Indicator ：
 D10 ： 24V power indicator, NO
 D9 ： 5V power indicator, NO
 D8 ： 3.3V power indicator, NO
Temperature Driving Board
77
Port introduce ：
 J3 ： communication port of Incubation carousel temperature
collection board
 J4 ： communication port reagent cooling temperature
collection board
 J20 ： Jumper port
 J21/J22 ： CAN communication port
 J18 ： trigger and wash buffer heater
 J16 ： Incubation carousel heater port
 J14/J15 ： power port, input 24V
 J12 ： Reagent cooler port
 J5 ： cooling fan port, non-feedback fan
 J9 ： cooling fan, water cooling floater port
 J13 ： Heating block assembly port
 J24 ： Water cooling pump port
Indicator ：
 D11/D13 ： 24V power indicator, NO
 D10 ： 5V power indicator, NO
 D9 ： 3.3V power indicator, NO
 D33 ： status indicator of reagent cooler. NO when cooling
 D2 ： flash when heating wash buffer
 D3 ： flash when heating trigger
 D4 ： flash when heating Incubation carousel
 D45 ： status indicator of water cooling cycle pump, NO
 Temperature Collecting Board
78
 Incubation carousel temperature collection board ： 2
side of Incubation carousel +trigger
 Reagent cooler temperature collection board ： 2 side
of reagent cooling+ wash buffer +environment
temperature
 Temperature display range 0~80℃ and constant  0.1 ℃
means short circuit, constant 80℃ means circuit open

Port introduce ：
 J3/J4/J5/J2 ： temperature sensor port
 J6 ： power and serial port communication

Indicator ：
 D1 ： 5V ， ON
 D6 ： 3.3V power indicator, ON
 D2 ： calibration indicator, on under uncalibrated, off under
calibrated
 D3 ： counter indicator ， flash
 D4 ： data transfer indicator, flash when data transfer
 D5 ： test indicator, NC when normal working
 Sample Sensor Board
79

Port introduce ：
 J3 ： power and communication port
 J4 ： sample rack indicator control port
 J6 ： Optocoupler port 1
 J7 ： Optocoupler port 2

Indicator ：
 D1 ： status indicator, flash when
working
 D2 ： reserved indicator, NC
 D3 ： Green, NO
 D4 ： Green, NO
 Liquid Level Detection Board
80
 Liquid level detection
 Vertical collision detection

Liquid level detection board

Indicator No. D1 D2 D3 D4
Color yellow yellow red green

No.
Cal. Indicate Sensitivity indicate
Main function Vertical collision Level signal
Dormant indicate Dormant indicate

1 Normally status Off On Off Off

2 Dormant status / Flash On Off
3 Liquid detected / On Off On
4 Sensitivity shift / On On Off

5 Probe collision alarm On / / /

6 Cal. Failure / Off Off Off

7 Board abnormal / On On On
8 Other abnormal Off Off Off Off
 Sample Rack Indicator Board
81

 Sample rack status indicator ： Used to indicate the current status of the samples loading channel, 10 indicator lights in total,
two kind colors each channel (yellow and green).

 Two lights off: no samples on sample loading channel

 The green light is always on: the sample has been put in, but it is not running and can be taken away
 The yellow light is always on: the sample already running and cannot be removed. If the sample rack is at scanning loading
sample area, this space will not be allowed to put a new sample rack, otherwise it will report the failure of recovery.
 The green light flash: the operation is finished and can be removed
 The yellow and green lights alternate flash: scanning the sample barcode or there is an error that can be taken away
 Yellow light running: during the cycle test, the X axis should move, and a period in advance indicates that the user is forbidden
to loading or unloading the sample. Until the X-axis is completed throughout the cycle, finish running the horse light and
restore the previous indicator.
 Sam/Rea Probe Clot Detection Board
82

 Every machine include two probe clot

detection board ： Sam/Rea probe clot
detection board+ MS 1 step probe clot
detection board
 MS= Magnetic Separation

Port introduce ：
 J2 ： Power and communication port
 J4 ： Sam/Rea probe clot pressure sensor port

Indicator ：
 D1 ： 3.3V power, NO
 D2 ： status indicator, on when working
 D3 ： error indicator, NC, on when initial error or
sensor error
PMT Calculating Module
83

 Input voltage is 12V

 Outputs 5V power supply for the PMT,
also acts as a switch for the PMT
 Collect, convert and transfer signals
picked up from the PMT
 Sensor
84
Reflex sensor
Optocoupler

Red

White

Green

Black

Voltage Red-black ：＜ 1.8V Voltage between 1 and 3: 5V±5%

Voltage green-white ： Blocking Voltage between 2 and 3: Blocking

＞ 2V （ Normal 4V ）， No ＞ 2V ， No blocking ＜ 0.8V(Short

blocking ＜ 0.8V （ Normal 0.2V) trip)

Voltage between 2 and 3:Blocking
＜ 0.8V ， No blocking ＞ 2V(Long
85