WOLAITA SODO UNIVERSITY

College of Natural and Computational Science

Department of Biotechnology
ANIMAL BIOTECHNOLOGY
Program Regular Biotechnology
Module name Agricultural Biotechnology
Module number 09
Course Title Animal Biotechnology
Course Code Biot3097
Instructor TESFAYE M. (M.Sc. in Biotechnology)
Course delivery The course will be delivered through lectures, class discussions, brainstorming,
system reading assignment, group discussion, exercises and Students taking this course
will be assessed through assignments, group discussion, presentation,
Self-reflection and final examinations.
ECTS 7
Contact Hours (Per Contact Effective Discu Home Library Assessment Total
Week) Hours Lecture ssion Based Work
Hours Activities
4 4x12=48hrs 14hrs 30hrs 30hrs 13hrs 135hrs
Cr. Hrs. 3(2+1)
Year / semester III/ I
Status of Course Core
Target Group 3rd Year Biotechnology Students
II. Course Description
The course deals with Animal Cells: cell culture media, types of cell culture, Primary and
established culture; organ culture, tissue culture; disaggregation of tissue and primary cell culture;
cell lines; cell growth kinetics, cell separation, cell counting, storage of cells, cryopreservation;
Animal cloning; Silkworm Biotechnology; Pest Management; Embryology: Gametogenesis and
fertilization in animals; Molecular events during fertilization; Genetic regulations during Embryonic
development; Collection and preservation of embryos; Culturing of embryos, Embryonic stem cells
and its applications; Artificial insemination, IVF, Reproductive and Therapeutic Cloning; Transgenic
animal production and application; recovery of products from animal cells.
Course Objectives
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At the end of this course the students will be able to:
 Understand the animal cell culture techniques.
 Understand the methods of isolating & maintaining good quality breeds of animals
 Know the principles and applications of transgenic animal development & animal cloning.
 Understands the principles & techniques of animal genetic engineering and immunotechnology.
Assessment Arrangements
Test 1 will be given, which weights out of 10%,
Test 2 will be given, which weights out of 10%
Assignment 1 will be given, which weights out of 10%
Assignment 2 will be given, which weights out of 10%
Attendance will be taken randomly, which weights out of 10%, and
The remaining 50 % will be for final exam.
Required Text Books and Materials
1. Albert et al., (1994) molecular biology of the cell, Garland publishers, New York
2. Cooper GM, (1997) The Cell. A molecular approach. ASm press, Washington
3. Lewin B (2000) Genes VII, Oxford university press, New York
4. Lodish et al., (2000) Molecular cell biology (4th Edition). W.H. Freeman & CO.
5. Gardiner et al., (1991). Principle of genetics, John wiley 7 sons PvtLtd.
6. Purochit S (2005) Biotechnology fundamental and application 4th ed.
7. Watson et al., (2004). Molecular biology of the gene (8th ed)

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 Course Content
Day Contents Reference Materials
1 CHAPTER 1. 1. B.D singh (2003)
Introduction Biotechnology , Kahani
Chapter 2. EMBRYOGENESIS publisher, New Delhi
2.1 Gametogenesis in animals
 Spermatogenesis
 Oogenesis
2 2.2 Fertilization in animals 2. Cruegar, W. and cruger, A
 Internal and External fertilization (1989) Biotechnology: A text
 Fertilization and Anti-fertilization book of Industrial
 Processes of fertilization microbiology 2nd ed: Panima
Activation of the egg publication corporation, New
 Amphimixis Delhi
 Kinds of fertilization
 Significance of fertilization
 Post fertilization changes in egg
 Molecular events during fertilization
 Genetic regulation during environgenic development
 Collection and preservation of embryos
 Cultivation of embryos
 Stem cells and other application
 Artificial insemination
3 CHAPATER 3: STRUCTURE & ORGANIZATION OF 3. Purohit ,S.S (2005)
ANIMAL CELL & TISSUE CULTURE Biotechnology: Fundamental and
3. Introduction application, 4th edition. Agrobios,
3.1. Definition India
3.2. History and scope of animal cell & Tissue culture
3.3. Advantages & disadvantages of tissue culture methods
3.4. Equipment & materials for tissue culture technology
3.5. Primary & established cell lines
3.6. Kinetics of cell growth
 Established cell lines
 Primary cell lines
 Interaction among cell lines
3.7. Genetics of cultured cells
 Metabolism
3.8. Animal tissue culture media
3.8.1 Natural media
 Blood plasma
 Blood serum
 Serum free media
3.8.2. Chemically defined media
3.8.3 Artificial media
4 3.9. Some characteristics of animal cell growth in culture. 4. Purohit,S.S (2005)
3.10. The substrates on which cells grow in lab. Biotechnology: fundaments and
 Treatment of substrate surface application ,4th edition. Agrobios,
 Feeder layer on surface India
 The gas phase of the tissue culture
3.11. Culture procedure
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  Preparation and sterilization substrates(apparatus)
 Preparation and sterilization of reagents and media
 Preparation of animal material
3.12. Primary culture , cell lines & clones
 Isolation of the explant
 Disaggregation tissues and primary culture
-Mechanical disaggregation
- Enzymatic “
- Aggregation by trypsin, by colagenese
 Separation of viable and nonviable cells
5 3.13. Evaluation of continuous cell lines Thieman & paladins, introduction
3.13.1 Maintenance of cell lines to biotechnology 2004
 Maintenance adherent cell lines
 Maintenance of cell lines in suspension
 Storage of cells
 Cryopreservation
3.14. Large scale cell culture in Biotechnology
 Monolayer culture
 Suspension culture
 Immobilized culture
3.15. Somatic cell fusion
3.16. Tissue culture
 Primary explanation technologies
 Shade or cover slip cultures
 Flask cultures
 Test tube cultures
3.17. Organ culture
 Culture of embryo organs
 Whole embryo culture
 Culture adult organs
3.18. Application
 Cell culture products
 Vial vaccines
 Interferon
 Hybridoma antibodies
 Monoclonal antibodies
6 3.19. Genetic counseling Duvey, R.C (1993). A text book
Invtro fertilization and embryo transfer technology of biotechnology. Schand &
 Invtro fertilization (IVF) in human company Ltd.
 Treatment of patents for IVF
-Collection of oocyte Grupta, P.K (2004) Elements of
- Collection of sperm Biotechnology. Rastogi
- Invitro fertilization publishers, India
3.20. Embryo transfer technology ( ETT)
 ETT in cells
 Application of ETT
 Limitation of ETT
3.21. Babies of specified sex
7 CHAPTER 4: GENETIC ENGINEERING IN ANIMALS Duvey, R.C (1993). A text book
-Animal cloning of biotechnology. Schand &
-Genome maps company Ltd.
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  The genome project
Mapping - Sequencing - Functional analysis Grupta, P.K (2004) Elements of
 Molecular Markers Biotechnology. Rastogi
-RE LP publishers, India
- Random amplified polymorphic DNA
- Variable number of tandem repeals
 Chromosome jumping
8  Chromosome walking -Duvey, R.C (1993). A text book
 Fluorescence in situ hybridization of biotechnology. Schand &
 Microsatellite (STR) mapping company Ltd.
Somatic cell hybrids -Grupta, P.K (2004) Elements of
 Genetic and physical maps Biotechnology. Rastogi
DNA finger printing publishers, India
9 Transgenic animals -Duvey, R.C (1993). A text book
 Introduction of biotechnology. Schand &
 Objective of gene transfer company Ltd.
 Vectors
 Gene transfer
-Promaliers
- Reporter or maker genes
- Trymidine kinas
- Dehydrofloete leducture
- XGRT
- Neomycin phosphotranfernese
Transfer methods
 Calcium Phosphate potation
 DEAE Dextratin dedicated transfection
 Lipofection
 Electroporation
 Retroviral infection
 Microinjection
-Polypeptide growth factors
10 -Embryonic stem cell transfer Grupta, P.K (2004) Elements of
-Targeted gene transfer Biotechnology. Rastogi
 Gene disruption publishers, India
-Transgene integration
-Detection of transgenics & transgene function
 Identification of transgenic animals
 Analysis of transgenic animals
 Assays of protein expression
11 Transgenic animals produce -winker, Ernst L. (1987) from
 Transgenic mice genes to clones, introduction to
 Cattle gene technology VCH, Newyork
 Goats
 Sheep -S.R primose (1994).Principles of
 Fish gene manipulation, and
Problems after developing transgenic animals introduction to genetic
Pest management
Transgeniods- Transgenic animal production & applications
Recovery of products from animal cells
- Cytokines
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 - Plasmogen activator
- Blood clotting factor
12 Immunotechnology Wiley-Liss, froshney, culture of
 Hybridoma technology and production of mono animal cells, 3rd edition
clonal antibodies
 Production of monoclonal Agg. (MBSs)
Fusion and culture of hybridomas
- Cloning & preservation of huybridones
- Production of Agg.
 Antibody engineering using genetic manipulation
 Uses of MoAbs (Monoclonal Antibodies) for
- Diagnosis
- Vaccines
Stem cells and their application
 What are stem cells and why they are important
 The unique property of stem cells
 Types of stem cells
 The potential uses of human cells in medicine
 Risks of stem cell therapy
Gene therapy
Ethical Issues in Animal Biotechnology

Approval section

Course Instructor
TESFAYE MENTA_____________________ __________________ ____________________
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Program Coordinator
__________________________ __________________ ____________________
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Department head
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