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Seed Germination Data Gathering and Observation

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24 views3 pages

Seed Germination Data Gathering and Observation

Uploaded by

drew09652271189
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Laboratory Exercise: Determining the Germination Rate of Mung Bean (Vigna radiata)

Seeds
Members:
Gregorio Jr Corpuz R.
Junrey Dumale
Ivy mae Dela Torre
Ivan lameda
Jerson Ibañez
Kian Lloyd Mesguilla
Kim Louie pahamutang
Julian James Escalera
Reymart Ranghus

Introduction

Seed germination is the process by which a seed sprouts and develops into a seedling. It's a
crucial stage in the life cycle of a plant, marking the transition from dormancy to active growth.
This seemingly simple process is actually a complex interplay of internal and external factors, a
fascinating dance between the seed's genetic blueprint and its environment.

The seed itself holds the key to germination. Its genetic makeup dictates its inherent
ability to germinate, its timing, and even its requirements. Within the seed lies the embryo, a
miniature plant waiting for the right conditions to awaken. The embryo is encased in protective
layers, often including a hard seed coat and stored food reserves (endosperm or cotyledons) to
fuel its initial growth. The seed's maturity and viability also play significant roles; an immature or
damaged seed may fail to germinate, even under ideal conditions.

Objectives

- Determine the percentage of viable seeds in a batch.

- Investigate the impact of different environmental conditions (temperature, light, moisture) on


germination rate.

- Identify optimal conditions for mung bean germination in a specific environment.

Materials Needed

Mung bean seeds (100 seeds)


Filter paper or paper towels
Distilled water
Spray bottle
Labels and marker
Notebook and pen
Calculator

Methodology

You will evaluate the germination rate of mung bean seeds under controlled conditions by
monitoring the percentage of seeds that germinate over a set period (e.g., 7 days).
Procedure
1. Preparation of Experimental Setup
Label each petri dish or shallow tray as T1, T2, T3, T4, and Control.
Place a filter paper or paper towel at the bottom of each dish.
Moisten the filter paper evenly with distilled water using the spray bottle. The paper should be
damp but not waterlogged.
2. Seed Placement
Count and place 10 mung bean seeds evenly in each rag dull.
Ensure the seeds are spread out to prevent overlapping.
3. Treatments
T1: Distilled water (control group).
T2: Slightly saline water (e.g., 0.5% NaCl solution).
T3: Tap water at room temperature.
T4: Slightly warm distilled water (30°C).
4. Monitoring and Recording Data
Keep the dishes in a well-lit area but away from direct sunlight. Maintain room temperature
(25–30°C).
Add water as needed to maintain moisture, ensuring consistent conditions for all treatments.
Observe and record the number of seeds that germinate after 24 hours. Germination is defined
as the emergence of the radicle (seed root).
5. Data Collection
Record the number of germinated seeds daily for each treatment in a table (see below).
Calculate the germination percentage using the formula:

Data Table
DAY
CONTROL
TREATMENT 1
TREATMENT 2
TREATMENT 3

1
15
10
12
18

Germination Percentage results:


1. Control: GP= (15÷25)x100= 60%
2. Treatment 1: GP=(10÷25)x100= 40%
3. Treatment 2: GP=(12÷25)x100=48%
4. Treatment 3: GP=(18÷25)x100=72%

Analysis Questions
Which treatment resulted in the highest germination rate? Why?
● T3 (Warm water) resulted in the highest germination rate (72%). Optimal temperatures
support faster activation of biochemical pathways crucial for seed development.
How did saline water (T2) affect the germination rate compared to the control?
● The germination rate in saline water (40%) was lower than the control (60%). Salinity
can cause osmotic stress, reducing the seed's ability to absorb water.
Explain the role of environmental factors such as water quality and temperature in seed
germination.
● Clean, fresh water is essential for proper hydration and nutrient transport during
germination. Contaminated or saline water creates osmotic stress, limiting water uptake
and hindering enzyme activity.
● Optimal temperatures (25–30°C for mung beans) enhance enzymatic reactions and
metabolic activities, leading to faster germination. Extreme temperatures, whether too
cold or too hot, can slow or inhibit germination by affecting cellular function.
Based on your findings, suggest optimal conditions for mung bean germination.
● Based on the findings, the optimal conditions for mung bean germination are maintaining
a temperature of around 30°C, using distilled or clean water, keeping the seeds
consistently moist without waterlogging, and placing them in a well-lit area away from
direct sunlight.

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