Lec.1 MEET THE EXPERT - PATHOLOGIST
Lec.1 MEET THE EXPERT - PATHOLOGIST
• Histology is the study of disease involving the knowledge of the basic structure of cells and organs.
- An understanding of the structure and function of cells as historically come from looking at
microscope slides of tissues and organs.
• The study of using microscopic slides to diagnose cancer is called histopathology.
• A very important part of diagnosing disease and tumours is what the organ or structure looks like
with the disease with the naked eye (or possibly by radiology, x-rays or CT scans). This is called the
macroscopic appearance or a gross image.
• when patients presents to their doctor with suspicious lumps or bumps, small tissue fragments are
taken from this by the physicians or surgeons. This is called a tissue biopsy. The tissue biopsy is fixed
in formaldehyde and sent to the Pathology Department.
• The next part of the process is that the tissue is embedded into paraffin wax to make it hard and to
preserve it.
• Then a formaldehyde fixed paraffin and wax embedded block is created
• The standard stain that we look at is called haematoxylin and eosin. This makes the nuclei purple
and the cytoplasm bright pink “for iron (Pearls stain), fibrosis (Trichrome stain).”
• immunohistochemical stains that target specific tumour markers that help us diagnose tumours.