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Lec.1 MEET THE EXPERT - PATHOLOGIST

Pathology is the study of disease, while histology focuses on the structure of cells and organs. Histopathology involves using microscopic slides to diagnose cancer, starting with a tissue biopsy that is processed and stained for examination. Key techniques include the use of standard stains like haematoxylin and eosin, as well as immunohistochemical stains for identifying specific tumor markers.

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0% found this document useful (0 votes)
5 views1 page

Lec.1 MEET THE EXPERT - PATHOLOGIST

Pathology is the study of disease, while histology focuses on the structure of cells and organs. Histopathology involves using microscopic slides to diagnose cancer, starting with a tissue biopsy that is processed and stained for examination. Key techniques include the use of standard stains like haematoxylin and eosin, as well as immunohistochemical stains for identifying specific tumor markers.

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• Pathology is the study of disease.

• Histology is the study of disease involving the knowledge of the basic structure of cells and organs.
- An understanding of the structure and function of cells as historically come from looking at
microscope slides of tissues and organs.
• The study of using microscopic slides to diagnose cancer is called histopathology.
• A very important part of diagnosing disease and tumours is what the organ or structure looks like
with the disease with the naked eye (or possibly by radiology, x-rays or CT scans). This is called the
macroscopic appearance or a gross image.
• when patients presents to their doctor with suspicious lumps or bumps, small tissue fragments are
taken from this by the physicians or surgeons. This is called a tissue biopsy. The tissue biopsy is fixed
in formaldehyde and sent to the Pathology Department.
• The next part of the process is that the tissue is embedded into paraffin wax to make it hard and to
preserve it.
• Then a formaldehyde fixed paraffin and wax embedded block is created
• The standard stain that we look at is called haematoxylin and eosin. This makes the nuclei purple
and the cytoplasm bright pink “for iron (Pearls stain), fibrosis (Trichrome stain).”
• immunohistochemical stains that target specific tumour markers that help us diagnose tumours.

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