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TS EN ISO 9308-1/A1
Mart 2017

ICS 07.100.20

Su kalitesi - Escherichia coli ve koliform

bakterilerin sayımı - Bölüm 1: Düşük bakterili
zemin floralı sular için membranla süzme yöntemi
(ISO 9308-1:2014/Amd 1:2016)

Water quality - Enumeration of Escherichia coli and coliform bacteria

- Part 1: Membrane filtration method for waters with low bacterial
background flora

Qualiteé de l'eau - Deé nombrement des Escherichia coli et des bacteé ries
coliformes - Partie 1: Meé thode par filtration sur membrane pour les
eaux aà faible teneur en bacteé ries

Wasserbeschaffenheit Zaä hlung von Escherichia coli und coliformen

Bakterien Teil 1: Membranfiltrationsverfahren fuä r Waä sser mit
niedriger Begleitflora

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 TS EN ISO 9308-1/A1 : 2017-03
ICS 07.100.20 TS EN ISO 9308-1/A1 : 2017-03
TÜRK STANDARDI
EN ISO 9308-1:2014/A1:2017
Milli Önsöz

Bu düzeltme, üç resmi dilde yayınlanan EN'nin eki olarak Ocak 2017 tarihinde CEN tarafından
onaylanan ve Türk Standardları Enstitüsü tarafından adapte edilerek yayınlanan TS EN ISO
9308-1:2014 standardının ekidir.

TS EN ISO 9308-1/A1 : 2017 standardı, EN ISO 9308-1:2014/A1:2017 standardı ile birebir aynı olup, Avrupa Standardizasyon Komitesi 'nin
(Avenue Marnix 17, B-1000 Brussels) izniyle basılmıştır.

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 TS EN ISO 9308-1/A1 : 2017-03

EUROPEAN STANDARD EN ISO 9308-1:2014/A1

NORME EUROPÉENNE
EUROPÄISCHE NORM January 2017

ICS 07.100.20

English Version

Water quality - Enumeration of Escherichia coli and

coliform bacteria - Part 1: Membrane filtration method for
waters with low bacterial background flora (ISO 9308-
1:2014/Amd 1:2016)
Qualité de l'eau - Dénombrement des Escherichia coli Wasserbeschaffenheit Zählung von Escherichia coli
et des bactéries coliformes - Partie 1: Méthode par und coliformen Bakterien Teil 1:
filtration sur membrane pour les eaux à faible teneur Membranfiltrationsverfahren für Wässer mit niedriger
en bactéries (ISO 9308-1:2014/Amd 1:2016) Begleitflora (ISO 9308-1:2014/Amd 1:2016)

This amendment A1 modifies the European Standard EN ISO 9308-1:2014; it was approved by CEN on 18 January 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION

COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9308-1:2014/A1:2017 E
worldwide for CEN national Members.

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 TS EN ISO 9308-1/A1 : 2017-03
EN ISO 9308-1:2014/A1:2017 (E)

Contents Page

European foreword....................................................................................................................................................... 3

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 TS EN ISO 9308-1/A1 : 2017-03
EN ISO 9308-1:2014/A1:2017 (E)

European foreword

This document (EN ISO 9308-1:2014/A1:2017) has been prepared by Technical Committee ISO/TC 147
“Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat
of which is held by DIN.

This Amendment to the European Standard EN ISO 9308-1:2014 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by July 2017, and
conflicting national standards shall be withdrawn at the latest by July 2017.

Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.

Endorsement notice

The text of ISO 9308-1:2014/Amd 1:2016 has been approved by CEN as EN ISO 9308-1:2014/A1:2017
without any modification.

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 TS EN ISO 9308-1/A1 : 2017-03
INTERNATIONAL ISO
STANDARD 9308-1

Third edition
2014-09-15
AMENDMENT 1
2016-12-15

Water quality — Enumeration

of Escherichia coli and coliform
bacteria —
Part 1:
Membrane filtration method for waters
with low bacterial background flora
AMENDMENT 1
Qualité de l’eau — Dénombrement des Escherichia coli et des
bactéries coliformes —
Partie 1: Méthode par filtration sur membrane pour les eaux à faible
teneur en bactéries
AMENDEMENT 1

Reference number
ISO 9308-1:2014/Amd.1:2016(E)

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 TS EN ISO 9308-1/A1 : 2017-03
ISO 9308-1:2014/Amd.1:2016(E)


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ISO 9308-1:2014/Amd.1:2016(E)


Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment,
as well as information about ISO’s adherence to the World Trade Organization (WTO) principles in the
Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.
Amendment 1 to ISO 9308-1:2014 was prepared by Technical Committee ISO/TC 147, Water quality,
Subcommittee SC 4, Microbiological methods.

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 TS EN ISO 9308-1/A1 : 2017-03

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 TS EN ISO 9308-1/A1 : 2017-03
ISO 9308-1:2014/Amd.1:2016(E)

Water quality — Enumeration of Escherichia coli and

coliform bacteria —
Part 1:
Membrane filtration method for waters with low bacterial
background flora
AMENDMENT 1
Page 2, Clause 4, second paragraph
Replace the second paragraph with the following.
Incubation of the membrane filter at (36 ± 2) °C for (21 to 24) h.
Page 3, 8.3, first paragraph
Replace the first paragraph with the following.
After filtration (8.2), place the membrane filter on the Chromogenic Coliform Agar (CCA) (B.1),
ensuring that no air is trapped underneath, invert the Petri dish, and incubate at (36 ± 2) °C for
(21 to 24) h.
Page 4, 11.2
Replace Table 1 with the following.

Table 1 — Performance testing of Chromogenic Coliform Agar

Function Incubation Control strainsa Reference Method of Criteria (Pro- Characteristic
medium control ductivity) reactions
Productivity (21 to 24) h/ E. coli
(36 ± 2) °C Dark-blue to
WDCM 00013 or TSA Quantitative PR ≥ 0,7
violet colonies
WDCM 00012
Ent. aerogenes
WDCM 00175 or Pink to red
TSA Quantitative PR ≥ 0,7
C. freundii colonies
WDCM 00006
Selectivity (21 to 24) h/ E. faecalis
(36 ± 2) °C Total or partial
WDCM 00087 or — Qualitative —
inhibition
WDCM 00009
Specificity (21 to 24) h/ P. aeruginosa Colourless
(36 ± 2) °C — Qualitative —
WDCM 00024 colonies
a Refer to the reference strain catalogue available (viewed 2016-10-28) on http://www.wfcc.info/pdf/WDCM_Reference_
Strain_Catalogue.pdf on culture collection strain numbers and contact details.

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ICS 07.100.20
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