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Tolen Test

Tollens' test, also known as the silver mirror test, is used to differentiate reducing sugars from non-reducing sugars. It works by oxidizing aldehydes present in reducing sugars using Tollens' reagent, reducing the silver ions in the reagent to form a metallic silver precipitate or "silver mirror" coating the test tube. The test involves adding the sugar sample and a blank to Tollens' reagent, then observing for the formation of a silver precipitate, which indicates the presence of reducing sugars.

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518 views7 pages

Tolen Test

Tollens' test, also known as the silver mirror test, is used to differentiate reducing sugars from non-reducing sugars. It works by oxidizing aldehydes present in reducing sugars using Tollens' reagent, reducing the silver ions in the reagent to form a metallic silver precipitate or "silver mirror" coating the test tube. The test involves adding the sugar sample and a blank to Tollens' reagent, then observing for the formation of a silver precipitate, which indicates the presence of reducing sugars.

Uploaded by

Mg H
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Benedict’s Test

Tollens’ test definition


Tollens’ test is a chemical test
used to differentiate reducing
sugars from non-reducing
sugars. This test is also called the
silver mirror test based on the
end product of this test.

2
Tollens’ test definition
Tollens’ test is a chemical test
used to differentiate reducing
sugars from non-reducing
sugars. This test is also called the
silver mirror test based on the
end product of this test.

2
Principle:
When an aldehyde is introduced
to the Tollens reagent, two thing
occurs. Firstly, the aldehyde is
oxidized by the Tollens reagent
and forms a carboxylic acid and
secondly, the silver ions present
in the Tollens reagent are
reduced into metallic silver.
2
Reagent
Tollen’s reagent: Add 50 ml of 0.1 M AgNO3 to a beaker and to this, add 25 ml of 0.8 M KOH.
Now, add sufficient volume of aqueous ammonia in order to dissolve the brown precipitate.
Test sample
Materials required
Test tubes
Test tube stand
Pipette

2
Procedure of Tollens’ test
Take two clean, dry test tubes and add 1 ml of the test sample in one test tube
and 1 ml of distilled water in another as blank.
Add 2 ml of Tollen’s reagent to both the test tubes.
Keep both the test tubes in a water bath for 1 min.
Observe the formation of color and note it down.

2
•The formation of a dark grey precipitate or silver mirror on the bottom and sides of the test tube
indicates a positive result, which means that the given sample contains reducing sugars/ aldoses.
•The absence of such precipitate indicates a negative result, which means that the test sample doesn’t
have reducing sugars/ aldoses/ α-hydroxy ketoses.

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