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Exercise 4 Metho

The document discusses an experiment that tested how molecular size affects the rate of diffusion in a colloid. Potassium permanganate, methylene blue, and congo red, which have different molecular weights, were diffused through a 5% gel colloid. It was hypothesized that larger molecules would diffuse more slowly. The results showed that potassium permanganate, with the lowest molecular weight, diffused the farthest, while congo red, with the highest weight, diffused the shortest distance, supporting the hypothesis.

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100% found this document useful (2 votes)
762 views19 pages

Exercise 4 Metho

The document discusses an experiment that tested how molecular size affects the rate of diffusion in a colloid. Potassium permanganate, methylene blue, and congo red, which have different molecular weights, were diffused through a 5% gel colloid. It was hypothesized that larger molecules would diffuse more slowly. The results showed that potassium permanganate, with the lowest molecular weight, diffused the farthest, while congo red, with the highest weight, diffused the shortest distance, supporting the hypothesis.

Uploaded by

Taggart Siao
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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Diffusion through a Colloid

Diffusion refers to the process by which molecules mix up due to their kinetic energy. It is also the process by which molecules spread from due to the differences in concentration. The experiment was designed to test the diffusion in different settings. The part that was discussed is the diffusion in a colloid. Colloids are mixtures whose particles are larger than the size of a molecule but smaller than particles that can be seen with the naked eye. This colloid is composed of H20 and 5% gel. This part of the experiment is created to address the question: Can molecular size affect rate of diffusion of particles in a colloid?

The tentative answer by which we all proposed is that the larger the molecular weight resulting in a large size of the molecules, the slower it would diffuse through the colloid.

Distance Traveled (cm) Time (min) 5 10 15 20 25 30 35 40 45 KMnO4 0.6 0.7 0.8 0.9 1.00 1.00 1.00 1.1 1.2 Methylene Blue 0.5 0.5 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Congo Red 0.3 0.4 0.4 0.4 0.4 0.4 0.5 0.5 0.5

50
55 60

1.2
1.2 1.3

0.6
0.6 0.6

0.5
0.5 0.5

The colloid used as a medium made it possible to observe the effect of molecular mass in determining the rate of diffusion. Since a gel has a porous framework, substances with a high molecular mass will travel slower. This is due to the pores trapping the particles of the solution thus slowing their diffusion. Potassium permanganate, methylene blue, and congo red have the following molecular weights: 158.034 g/mol, 696.665g/mol, and 319.85 g/mol respectively.

It was hypothesized that molecules with smaller masses diffuse slower than those with higher molecular weights. In this table, it is clearly seen that KMnO4 traveled the farthest among the three solutions and Congo red has the least travelled distance. Looking at the molecular weights, KMnO4 has the lightest while the Congo red has the largest. With this information, it can be concluded that the larger the molecular weight resulting in a larger particle size of the substances molecules, the slower it will travel over a porous framework, e.g. a colloidal gel thus confirming the given hypothesis by the students.

However, it should be noted that the distribution of gel in the colloid is unequal. This results to the stopping of the solution at a certain distance.

This part of the experiment dealt with the effect of molecular weight on the rate of diffusion. By utilizing three reagents potassium permanganate (KMnO4), methylene blue and congo red with different molecular masses, to show the differences in the diffusion rate in a colloidal setting.

By observing and tabulating the data gathered from the experiment, it was found that potassium permanganate diffused the farthest along the colloid which was followed by methylene blue and congo red, respectively. By relating the masses and the size of the molecules with their diffusion along the gel, it can be seen that the lower the molecular mass, the less it travels, the slower the diffusion rate.

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