5

HumaLyte Plus
3
HumaLyte Plus
| Service Manual

|
Cat.No. 17470/2
 Revision List of the Manual
No. Rev./DATE REVISION DESCRIPTION
01 01/2010-11 First edition

Copyright
Copyright 2010, Human GmbH, Wiesbaden, Germany. All rights reserved.
No part of this documentation may be reproduced in any form, nor processed, copied or distributed by means of
electronic systems, without prior permission of Human GmbH in writing. Since all precautionary measures were
taken into account in producing these operating instructions, the manufacturer accepts no responsibility for any
errors or omissions. This includes any liability for damage that could arise from possible incorrect operation based
on this information. Subject to changes without notice as result of technical development.

Service and Support

i
ii
 Preface
In order to operate and service the instrument properly, please read the operation manual and service manual
carefully before operating. When receiving the goods, please check the product model and spare parts against the
packing list. If missing or mistake, please contact the supplier immediately. Non-authorized personals are not
allowed to open or repair the instrument. The instrument must be grounded properly. Never connect the plugs of
the rear panel with electric metal materials. Always remember to disconnect the power cable from the instrument
before open it. Do not remove any symbols from the instrument. The waste may contain materials harmful to
human body. It should be discarded according to the safe laboratory procedures and government regulations.

1. System introduction

1.1.1 Front panel Thermal

printer

Sampler
mechanics

Touch screen

Positioner

Electrode
Liquid distribution
assembly
valve

Auto Sampler
tray

Pump head Peristaltic

tube holder
Auto Sampler
bottom tray

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1.1.2 Rear panel
1 Power switch
2 Power socket (including fuse holder)
3 RS-232 serial port
4 Grounding pole
5 Machine label

1.2 Environment requirements

1 The instrument should be installed on a stable and solid platform that is free of mechanical vibration and
away from vibration source.
2 The environment should be as free as possible from dust, corrosive gas, loud noises and electrical
interference.
3 Avoid placing the instrument in direct sunlight or in front of a source of heat or vent.
4 Ambient temperature: 10~35, relative humidity:20%-85%.
5 The power supply should be 110/220/110VAC 10%, 50/60 Hz
6 Power supply and grounding should be connected correctly.
7 No strong electric field and magnetic field interference.

1.3 External Connections

1 Power cable: should be connected to the power socket firmly and reliably without loose or poor connection.
2 Grounding: the grounding pole should be connected to ground properly. The grounding wire should be as
short as possible and the grounding resistance must be less than 10
3 If the power supply to the instrument is not stable, or there are high-power equipments on the same line,
then a power stabilizer (output 100W) is strongly suggested.
4 Avoid operating the instrument under high humidity environment for long time; otherwise the instrument
performance may be affected. Use desiccant or air conditioner in case required. Do not place the
instrument near water.

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1.4 Internal Connections

1 Press the front door to open it. The tubing and electrodes have been installed at the factory, the operator still
need to check them before using.
2 Check the electrode assembly: take out the electrode assembly from the accessory kit. Check if the setting screws
are tightened, inlet and outlet port are clean, the filling solution of each electrode is sufficient. If there are
bubbles on the electrode probe, tap the electrode slightly to let the bubbles disappear.
3 Installation of the electrode assembly: insert the whole assembly to the mounting base, connect the lead wire of
each electrode (KNaCaPHCLRef) to the plug of amplifier board correctly. Connect the tube to the inlet
and outlet port of the assembly.
4 Check the liquid positioner: replug the positioner and make sure it is well connected.
5 Check the tubing: make sure all the tubing are connected tightly and there is no leaking.
6 Insert the reagent pack.
7 Installation of printing paper: open the printer cover, insert the printing paper into the guide slot (the thermal
side face down), and feed the paper out from the slot on the printer cover.

Note:
Make sure all tubings are connected firmly and there is no leakage.
Make sure the positioner is connected firmly.
Make sure all the lead wires of the electrodes are connected firmly.
Make sure there is no bubble on the electrode probes.
The printing paper is using out when you see two red lines on it. Change the printing paper in time.

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2. Power-on check

2.1 Self-test and adjustment

When switch on the power to the instrument, system self-test will be performed automatically. The installation
personnel or operator may be required to do some adjustments to ensure the instrument under good conditions.

Operation Function properly Possible problems

Switch on the power, the - External power supply, power switch, - External power supply, power
LCD light on. internal power supply works properly - switch connections or fuse is not
LCD and cables works properly good - Internal power supply
doesnt work properly
Detect the positioner, - The positioner works properly - The positioner is loose.
printer and auto sampler - The printer works properly - Auto - The printer cables or internal
(if have) sampler works properly power supply is not good
- The system will not give alarm or
stop even if errors found on the
positioner or printer.
The operator should turn off the
instrument manually and solve
the problems.
Feed paper and print-out - The power supply and paper feeding - Internal power supply is not
the system version of the printer is good - Data good
transmission of the printer is good - The printing paper is not
installed correctly.
- The printer cables are loose or
not good.
Sample probe moves up - The lifting motor, limit switch and - The lifting mechanism is not
until it touches the cables work properly. good
position limit switch, then - Check if the sample probe is bend or - Adjust or replace the sample
moves down to the wash out of position during the movement probe if necessary
block
Calibrating - The liquid distribution valve and - The system gives alarm and
optical coupler, lifting mechanism, stops if errors found on liquid
switches and software work properly distribution valve, lifting
mechanism or switches
The instrument aspirates - Pump motor works properly - The - Pump motor is malfunction -The
Std B and Std A in turn for tubing system is good if Std B and Std liquid cannot reach correct
test and display the A can be aspirated into the position, showing the tubing
voltages distribution valve correctly -Details of system is leaking - The inlet tubes
voltage display and print please refer for Std A and B are connected to
to the operation manual the wrong ports - Std A or B
-The operator can turn off the function cannot be aspirated
of voltage display or print by extended
program

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Screen display after - All electrodes, Std A, Std B, tubing - If slope is not stable, then it
calibration: connections, pump, valve, signal shows shift - If slope is
Slope K 54.5 OK detection and data processing work abnormal, then it shows X -
normally Refer to trouble-shooting for the
Na 52.3 OK
reason of unstable or abnormal
Cl 51.6 OK slope.
Ca 25.5 OK
pH 55.6 OK
The system enters into Main Menu Sample Cal. W. List STD
main menu after self-test Service Results
If there is no operation for - the internal clock works normally -The internal clock is malfunction
more than 2 minutes, the if the system cannot enter into
system will enters into standby mode
standby mode to maintain
the electrodes.

Note:
Try the other side of the printing paper or change the whole paper roll if there is no printing on the paper. Adjust the
position of the paper if it is misalignment. If the sample probe is found distortion, misaligned or bended, the
operator should adjust or change the probe and make sure it can move into the wash block correctly. If the
aspiration is abnormal, the operator should check the tubing connections, reagent connections and electrode
connections and make sure there is no blockage or leakage. Activate the electrode for 10-30 minutes before using if
the electrode has been stored or not used for long time. Run calibration when the electrode is stable.

2.2 Prompts during self-test and calibration

2.2.1 Display Prompts

Code Error message
1# The optical coupler of liquid distribution valve failed
2# Elevator switch failed

2.2. Print Prompts

Code Error message

Error 0# Liquid positioning failed

Error 1# Liquid detecting failed

Warn 2# Bubbles detected

Error 3# Too much or less sample aspirated

Error 4# Slope abnormal

Error 5# Slope unstable

Error 6# AB Slope abnormal

Error 7# AB Slope unstable

For the solutions please refer to Trouble-shooting

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3. Function description and operation

3.1 Flow chart of the operation

Please refer to the operation manual.

3.2 System functions

Please refer to the operation manual.

3.3 Extended programs:

Press Service in the main menu, press Time and enter 983, press Exit, then the system will enter into extended
program menu, the screen displays:

(Attention: the extended programs are not made for the end-users to avoid wrong setting)

3.3.1 Print voltage

Press Pr. Vol , the button turns white. The voltages of the liquid positioner and all electrodes will be printed out
during calibration or sample measurement. Press Pr. Vol again, white color disappear from the button. The voltages
will not be. This is the default setting.

3.3.2 Print pH value

Press Pr. PH , the button turns white. The pH value will be printed out when the sample measurement is finished.
Press Pr. PH again, the button turns dark. The pH value will not be printed. This is the default setting.

3.3.3 Print TCa

TCa is a calculated parameter. Press Pr. TCa, the button turns white. The value of iCa and TCa will be printed out
when the sample measurement finish. Press Pr. TCa again, the button turns white. Only the value of iCa will be
printed out when the sample measurement finish. This is the default setting.

3.3.4 Adjust pH
Factory adjusted no need to adjust it again.

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3.3.5 Standby mode setting
The instrument will enter into Standby mode if there is no operation in 2 minutes. In standby mode, the operator
should press Wake Up to activate the instrument to the working mode. If the instrument stays in standby mode for 4
hours, it will maintain the electrodes automatically. When wake up the instrument, it will carry out a calibration
before returning to the working mode. The operator can change the waiting time for entering into standby mode.
The suggested waiting time is 2-60. To change the waiting time, press Standby, input the value, and then press Yes
to confirm.

3.3.6 Test Valve

The liquid distribution valve may be blocked or leaking after using for a long time. The measuring results could be
affected. Therefore it is necessary to check the valve periodically. Touch Valve, the system will aspirate Std A and Std
B automatically. Observe the aspiration volume, flow speed, the switch of Std A / Std B in the valve and the bubbles
to determine if the valve is working properly or not. (Details please refer to trouble-shooting)

3.3.7 Show
Press Show mv, the button turns white. The voltages of the liquid positioner and all electrodes will be displayed on
the screen during calibration or sample measurement. Press Show mv again, the button turns white. The voltages of
the liquid positioner and all electrodes will not be displayed on the screen during calibration or sample
measurement.

3.3.8 Setup the reference range

Touch Range, the screen displays:

K 1 xxx 2 xxx Na 3 xxx 4 xxx

Cl 5 xxx 6 xxx iCa 7 xxx 8 xxx
TCa 9 xxx 10 xxx PH

Set Exit

Press Set , input all the reference values, then press Exit to quit.

3.3.9 Record of de-protein operation

Press Pr. Clea to print out the last date that the de-protein program been performed.

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3.4 Routine operation

3.4.1 Safety precautions

The electric voltage inside the instrument may be harmful to human body. Do not open the instrument before
disconnect it from the power supply.
Because the samples may have pathogenesis bacterium or viruses, all replaced tubes, electrodes and waste
containers should be discarded according to the safe laboratory procedures and government regulations.
The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab
coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.

The reagents are harmful to human body. If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go to see a doctor.
If the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go to see a
doctor.

3.4.2 Sample Collection and handling

Sample collection and handling must be carried out by the professionals. Always avoid the Hematolysis. In addition,
the following points should be noted:
1 The serum or plasma can be stored in the refrigerator, but they must be warmed up to the room
temperature before test.
2 When preparing the blood serum samples, do not add any materials like the surface active agent that may
interfere in the measurement or even damage the sensor.
3 Hemolysis can affect K result significantly as well as other parameters. Special remark should be made for
hemolysis sample, and the results are for reference to the lab only.
4 If the serum does not been separated completely, the fibrins in the sample may affect the aspiration, and
the proteins may deposit inside the tubing thus cause contamination or blockage. In such case, the results
will be affected, so the operator should clean the tubing in time.
5 The sample should be tested within 2 hours, otherwise the result may be not accurate.

3.4.3 Operation precautions

1 The analyzer is designed to work continuously for 24 hours a day. No need to shut down the machine every
day.
2 Do not use the standard solutions for flame luminosity. They include strong acid and other supplements
that may damage the electrodes.
3 Not all commercial controls are suitable for ISE measurement. Some of them contain too much chemical
additives that may interfere in the measurement.
4 Bubbles should not be aspirated during the sample aspiration; otherwise the results will be unreliable.
5 When the sample reaches the checking point, make sure there are no bubbles inside; otherwise the sample
should be measured again.
6 Never aspirate the sample directly from the blood separating tube, because it is very easy to block the
tubing system. Always move the serum to a small and clean tube or cup with a pipette before testing on
the instrument.
7 If the ambient temperature fluctuates for more than 10, the instrument should be calibrated again.
8 The pH value of the standard solutions and the samples should be within 6-9, otherwise it will interfere in
the measurement of sodium ions.
9 Discard the reagent if mildew or deposition found.
10 Perform the routine maintenance according to the manual.
11 Every electrode has a serial number. Keep it on the electrode. The warranty to the electrode will become
invalid if the number cannot be recognized.

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12 When remove the sample tube after aspiration, be careful not to hit the sample probe, otherwise the probe
will bend or misaligned and cannot move into the wash block properly.
13 Do not open the front door during calibration or sample measurement; otherwise the result will be
affected.
14 Check and make sure the grounding is good if static interference is found

3.4.4 Daily operation

Please refer to the operation manual.

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4. Routine maintenance

4.1 Daily maintenance

Keep the instrument clean, especially the sampling unit.

4.2 Weekly maintenance

1 Check the electrodes and make sure the filling solutions are sufficient. Add filling solution if the volume is
less than 2/3.
2 Check if there is salty crystal on the internal electrode, if so, clean it with wet tampon
3 Run deprotein program in service menu. (If less than 5 samples per day, then just run deprotein program
every 2 weeks) Check and clean the tubing system.
4 Run Na adjust program if the slope of Na electrode is less than 50.

4.3 Monthly maintenance

1 Check the tubing system and make sure there is no blockage or leakage.
2 Check the internal electrode and make sure it doesnt blanch or its coating doesnt flake off. Change the
internal electrode if necessary. Check the filling solution; change it if it becomes feculent.
3 Observe the voltage of the electrode. If the voltage of K, Na, Cl are all less than 20 mV, then change the
reference filling solution first. If it doesnt improve, change the reference membrane.
4 Run calibration if the slopes are low.

4.4 Check tubing system

Check the tubing system if the aspiration volume or flow speed is abnormal, or bubbles found. Observe the flow
speed, liquid position and bubble status during sample test or calibration.
1 Blockage will cause insufficient aspiration volume for sample, Std A and Std B, or no aspiration at all if the
blockage is very bad.
2 Leakage will cause bubbles or no aspiration.
3 A segment of air will appear in the tube when the liquid distribution valve switches between Std A and Std
B. When aspirating Std A, if small bubbles found at the outlet of distribution valve, then the flow path
inside the distribution valve for Std A is leaking. If bubbles always stay at the inlet of Std A and cannot be
excluded, then the flow path for Std A is blocked slightly. Check flow path for Std B by the same way.
4 The blockage in the distribution valve is usually caused by floccules in Std A or Std B. It is important to keep
the reagents under proper temperature.
5 The bubbles will appear near the connector if it is leaking. Tighten the tube or connect it again. Cut a small
piece of tube if necessary.
6 If leakage found between the electrodes, then the O ring between the electrodes may miss or out of
position, or the setting screws are not tightened.
7 If the pump tube conglutinated, distortion, leaking or aged, it will cause no aspiration, insufficient
aspiration volume or incorrect liquid position. Change the pump tube if necessary.
8 Wash the tubing system regularly to prevent the blockage caused by protein deposition. Clean the external
of the sample probe. Clean the wash block.
When the instrument cannot aspirate, check the tubing system with Valve program in Service menu
Pull out the liquid outlet tube from the mixing chamber and put it into a waste container temporarily. Check if there
is blockage and leakage at the segment in front of mixing chamber.
Pull out the liquid outlet tube from the mixing chamber and put it into a waste container temporarily. Check if there
is blockage and leakage at the segment in front of mixing chamber.

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1 Take off the pump tube, check if it is conglutinated, distortion, leaking or aged. Check if the tubing
connector is blocked. Check the connection between pump tube and waste tube. Put the waste tube into
water, disconnect the connecting tube from the outlet of electrode assembly, and rotate the pump head
clockwise by hand. If the water can be aspirated normally, then the pump is working well. Otherwise check
and change the pump if necessary.
2 Put the waste tube and connecting tube back. Disconnect the aspirating tube from the sample probe and
put it into water. Rotate the pump head clockwise by hand. If the water can be aspirated normally, then the
electrode assembly is working well. Otherwise disassemble the electrode assembly for checking.
3 Put the aspirating tube back. Take off the tube from the outlet of the distribution valve and put it into
water. Rotate the pump head clockwise by hand. If the water can be aspirated normally, then the sample
probe is working well. Otherwise check the sample probe.
4 If the results of the above checking are good, then most likely the distribution valve is malfunction. Change
the valve if necessary.
5 If the segment in front of mixing chamber is normal, then the blockage and leakage should be happened in
the mixing chamber. Blockage is usually in the outlet at the bottom of mixing chamber or the stainless
steel tube for sample injection. Leakage is usually caused by -waste tube of mixing chamber broken -liquid
outlet valve closed unfirm -press tube of outlet valve distortion or broken -Four stainless steel tubes on the
cover of mixing chamber not stuck well and leakage -Air outlet valve broken -The tube connected to four
stainless steel tubes on the cover of mixing chamber broken.
6 Cleaning the mixing chamber Check all the tubes every 1-3 months. Replace the tube which is broken. The
breakage is usually caused by the aged tube or incorrect cleaning and repairing.
Blockage is usually caused by the serum protein deposit. Regular cleaning is necessary. Clean the mixing chamber by
the following steps.
Pull out the white tube connected to reagent R, which is in the middle of the mixing chamber, then inject 3ml
sodium hypochlorid solution into the chamber, wait for 5 minutes, put the tube back Pull out four tubes on the
mixing chamber cover straightly upward, loose the three setting nuts, take out three bolts and three nuts. Pull out
mixing chamber cover, clean the chamber and all the protein deposit on the outlet. Clean the protein deposit under
the cover, clean all four stainless steel tubes, cleaning mixer. Put the mixer back with small side downward, insert
the mixing chamber cover (pay attention to the direction of four stainless steel tubes), and tighten three nuts.
Connect the Std R tube to the inlet in the middle of the reaction chamber, and then connect the liquid outlet tube
(smallest tube), sensor connection tube, and air outlet tube. Do not damage the tube.

Warning:
Pay attention to the biological pollution by the sample when connect to the external waste bottle temporarily.

4.5 Check the sample probe

Adjust the sample probe if it is distortion, misaligned or bended. Make sure the sample probe can move into the
wash block smoothly and correctly.
If the sample probe is blocked, move it up to top position, take the probe out, and use a thin needle to remove
the blockage from the probe.
Dispose the waste instantly . Or add antiseptic into waste bottle in advance. z Wear the protection glove when clean
the mixing chamber. Do not touch serum protein deposit. Wash and disinfect your hand after cleaning the chamber.
If the wash block is blocked, lift up the sample probe, remove the cover of the wash block, take out the rubber and
clean it.

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4.6 Electrode maintenance and replacement

4.6.1 Structure of the electrode

4.6.2 Replacement of internal electrode and filling solution

Follow the steps below to replace the internal electrode or filling solution:
1 Pull out all electrode wires from the plugs. Remove the tubes from the inlet and outlet of the electrode
assembly.
2 Loose the fixing nuts of the electrode assembly.
3 Disassemble the whole electrode assembly.
4 Discard the electrode to be replaced.
5 Take a new electrode and add the filling solution.
6 Assemble the electrodes in correct order. Make sure that the rubber gaskets are in right position. 7.
Tighten the fixing nuts of the electrode assembly.
8 Connect the tubing to inlet and outlet. Insert the electrode wires back to the plugs.
9 Run calibration again.

If the internal electrode blanches or its coating flakes off, then remove the old filling solution, add fresh filling
solution and install a new internal electrode.

Change the filling solution if it is insufficient or the slope of the electrode is too low. Take out the internal electrode
(do not touch any metal materials), remove the old filling solution, add fresh solution to 85% of total volume, then
put the internal electrode back and tighten it.

K filling solution is for K electrode only, Ca filling solution is for Ca electrode only, and reference filling solution is for
reference electrode only. Na/Cl filling solution is for Na, Cl and pH electrodes.

4.6.3 Replace the reference membrane

Follow the steps below to replace the reference membrane:
1 Pull out all electrode wires from the plugs. Remove the tubes from the inlet and outlet of the electrode
assembly.
2 Loose the fixing nuts of the electrode assembly
3 Take out the reference electrode.

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4 Loose the screw cap, take out the internal electrode, remove the filling solution, O-ring and old reference
membrane (see the following figure).
5 Soak the new reference membrane in distilled water, then place it evenly on the internal interface and put
on the O-ring.
6 Refill the reference filling solution and tighten the screw cap
7 Clean and dry the electrode.
8 Assemble the electrodes in correct order. Make sure that the rubber gaskets are in right position
9 Connect the tubing to inlet and outlet. Insert the electrode wires back to the plugs.
10 Run calibration again.

4.7 Maintenance of pump, valve and lifting unit

4.7.1 Maintenance of aspirating pump and Lactic acid pump

After long time of using, the pump tube will get aged and may cause insufficient aspiration volume, therefore the
accuracy of the results will become poor. If so, it is necessary to change the pump tube.
If there is big noise when the pump head rotating, disconnect the pump tube, pull out the pump head, loose three
setting screws, take out the plate, add a little lubrication oil to the ball bearing, then assemble the pump head back.
If the pump head is damaged, then change it with a new one.

4.7.2 Maintenance of distribution valve

The function of the distribution valve is to switch the flow path to Std A or Std B according to the control program.
The valve status is detected by an optical coupler. The performance of the optical coupler can be affected by dust or
foreign objects, and may cause incorrect switch between Std A and B. In such case,1#2# or 3# error message
will show on the screen and be printed out. Clean the optical coupler periodically.
Disassemble the valve: Loose four setting screws on the valve plate, pull the whole valve out a little, loose four nuts,
take out the valve plate and four copper standings, loose the setting screws for the optical coupler. Take out the
optical coupler. Turn the valve head with a tweezers and take it out. Loose two screws on the valve cap and take the
cap out. Clean five O rings on the valve cap. Clean the valve body and rotor.
Assemble the valve: Install the rotor and cap to the valve body, tighten two screws. Pay attention to the five O rings
on the cap. Install the optical coupler to center position. Put on the valve head, tighten the screws on the valve motor
axis. Put the copper standings back, tighten the valve body to the plate. Tighten the valve plate to instrument.
Note: Pay attention to the position of the O-rings, copper standings, optical coupler and connectors for Std A and
B. Maintain the valve once every year. Keep the optical coupler clean and check it periodically.

4.7.3 Lifting unit

The maintenance of the lifting unit is to remove the dust, foreign objects and oil dirt from it. Remove the cover of the
sample probe, remove the sample probe, clean the axis and add a little grease. Let the sample probe move up and
down for several times, make sure it moves smoothly and low noise.

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Note: The electrode should be sealed and stored under dry environment. Do not add filling solution to it. The
internal electrode, tubing and O-rings should be sealed and stored under dry environment.

5. Hardware

5.1 Connector and Cable list

Connector Cable Connector Cable

J101 Optical coupler cable J203 Positioner cable

J102 Elevator switch cable J204 Signal cable

J103 Auto sampler home position
J205 RS232 cable
optical coupler cable
J104 Auto sampler counter optical J206 Pressure sensor cable
coupler cable
J107 Transformer output cable J207PRT Printer data cable

J108 Printer power cable J207Key Touch screen cable

J109 Air outlet valve connection cable J208 LCD cable

J110 Liquid outlet valve connection NBQ LCD inverter power cable
cable

J111 Mixing motor power supply cable

J112 Auto sampler motor cable

J114 Lifting motor cable

J115 Valve motor cable

J116 Aspirating Pump motor cable

J117 Lactic acid pump motor cable

5.2 PCB errors

1 Because of vibration or dust cumulating, the connectors or IC chips may have cold connections.
Disconnect the power cable, open the rear cover, and check the cables and IC chips. - 21 -
2 When switch on the power, the LCD doesnt light on, the lifting unit doesnt work, the pump doesnt work:
check 9-pin DC power cable and transformer output cable J107.
3 When switch on the power, the LCD doesnt light on, but the lifting unit work normally: Check U102
(LM7812), 9-pin DC power cable, NBQ connector and 12V voltage. The LCD inverter may be damaged.
4 The lifting unit cannot move, the valve doesnt work: Check U103 (LM7812), 9-pin DC power cable, U106,
U107
5 The pump motor doesnt work: Check U110LM7812, 9-pin DC power cable, U108, U109
6 The signals of calibration or measurement are confused or all zero: check cable J204.
7 If the program works incorrect, clean the dust cumulating around U301 IC chip and check. It is
recommended to clean with PCB washing solution (pure alcohol) and blow to dry
8 Auto sampler doesnt move; check U104, 74HC373, U105, ULN2803A
9 No AB test result, check J206 connector and U209, LM324.

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10 No print. Check J108, J207 (PRT) connectors and the connectors between printer head and print control
board. Replace the printer head if it is broken.
Note: Only authorized personals are allowed to repair the PCB.

5.3 Description of RS-232 interface

The analyzer can send data via RS-232 interface.

5.3.1 Features
Electric feature comply with EIA RS-232C
Transmission mode asynchronism
Stop bit1 digit Data bit8 digit
Parity bitnone transmission
Speed 19200 baud

5.3.2 Pin layout

Pin no. Signal Function Note
1 NC none
2 RXD Receive data Input
3 TXD Transmit data Output
4 NC none
5 GND signal ground
6 NC none
7 NC none
8 NC none
9 NC none

5.3.3 Data format

The instrument communicates with external PC through RS-232 serial port. After each test finish, the data will be
automatically sent out during the time of printing. The content of the transmitting data is almost same as the
printing data. The operator can also send out the data manually by Send program in Service menu.

Seq. ID Flag K Na Cl Ca PH AB Newline

No. byte result result result result result result
001 111111 063 5.23 151.2 111.4 1.44 7.62 12.0
004 2224 063 5.25 151.6 110.8 1.4 7.59 42.0
Example: 001 111111 063 5.23 151.2 111.4 1.44 7.62 12.0 004 112224 063 5.25 151.6 110.8 1.40 7.59 42.0 Data
format: Sample number + 2 blank + ID + 2 blank + symbol byte + blank + k result + blank + Na result + blank + Cl
result + blank + Ca result + blank + PH result + blank + AB result + newline
Remarks
1. means blank
2. Flag byte There are 8 digits for the flag byte:

h g f e d c b a

a=1 means K electrode is failed to pass STD

a=0 means: K electrode passes STD
b=1 means Na electrode is failed to pass STD
b=0 means: Na electrode passes STD

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b=1 means Cl electrode is failed to pass STD
c=0 means: Cl electrode passes STD
d=1 means Ca electrode is failed to pass STD
d=0 means: Ca electrode passes STD
e=1 means pH electrode is failed to pass STD
e=0 means: pH electrode passes STD
f=1 means AB is failed to pass STD
f=0 means: AB passes STD

h=1 means an error affecting all the testing results, such as aspiration abnormal or there are bubbles inside the
sample.

Example:
pH electrode has failed to pass STD while other electrodes pass STD, the flag data is 016
No sample or insufficient sample is aspirated, the flag data is 128

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6 Trouble-shooting

6.1 Error prompts

Display prompts
Code Error Solution

1# Optical coupler error Check J101, clean optical coupler, adjust the position of
optical coupler

2# Elevator switch error Check J102, J114, elevator switch, U103(LM7812),

U107(ULN2803), U106(74HC373), 9-pin DC power cable

Printing prompts
Code Error Solution

Error 0# Liquid positioning failed Connect the positioner again

Error 1# Liquid detecting failed Check the optical coupler and tubing connection of the
distribution valve
Error 2# Bubbles detected Check the positioner, optical coupler, distribution valve, check
if there is any leakage in the tubing sstem
Error 3# Too much or less sample Check the distribution valve and positioner
aspirated
Error 4# Slope abnormal Refer to 6.5 Slope abnormal
Error 5# Slope unstable Refer to 6.4 Slope unstable

6.2 Possible causes of errors

1 Power supply not
May cause the instrument works abnormally; the slopes become abnormal during calibration; the testing
result not stable
2 Grounding not good
May cause result is not stable during testing or calibration, especially for Na.
3 Interfere by electromagnetism
May cause: the instrument cannot work normally; result not stable during testing or calibration
4 Interfere by static and dry
May cause: instrument works unstable, the deviation of Na, Cl is too big
5 Interfere by moisture
May cause: instrument works unstable, the deviation of Na is too big
6 Interfere by temperature
May cause: instrument works unstable.

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7 Positioned not good
May cause: liquid position not accurate, prompt bubbles frequently, slope abnormal or unstable, testing
result unstable
8 Flow path in the electrode assembly contaminated
May cause: slope abnormal or too low
9 Std A or B contaminated
May cause: slope not stable or too low
10 Std A or B contaminated
May cause: slope not stable or too low
11 Reference electrode not good
May cause: slope not stable, testing result not stable
12 Flow channel not good
Check segment by segment for proper alignment and cleanliness.

6.3 Trouble-shooting of positioner and electrode

Positioner:
First, make sure the connection of the positioner is good. Then check the positioners voltage with Voltage
program in Service menu. Rotate the pump manually and let the liquid pass through the positioner. The
positioners voltage should become small when there is liquid or big when there is no liquid. The difference should
be more than 500.
Electrode:
Check the voltage of the electrode by Voltage program in Service menu. If the voltage is low, then the electrode
should be replaced as soon as possible. If aspiration alarm appeared or no aspiration at all, replace a new reagent
pack (Std A, Std B, Std R). Restart the instrument and run self-test.

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6.4. Slope unstable
Cause Solution
Unreliable grounding Check the connection of the grounding wire
No standard A or standard B aspirated Check and replace Standard A or B; Check the tubing connection

Incorrect positioning Adjust positioning again

The reference filling solution or reference Replace when necessary
membrane not working
The internal electrode turns gray Replace when necessary
Poor connection of the electrode wire Check and connect again
Electrode membrane leaks Replace when necessary
Power supply voltage fluctuates Use UPS or power stabilizer
Humidity too high Lower the humidity or move the instrument to a dry place
Bubbles inside the tubing Check the tubing system
Liquid leakage inside the valve Replace gasket or valve when necessary
The electrode is not activated or the activating Activate the electrode first
time is insufficient
Mixing Chamber Blocked Clean Mixing Chamber

6.5 Slope abnormal

Cause Solution
Too many organic deposits on the electrode
Wash it with de-protein Solution
membrane
Reagent contaminated Replace the reagent
Insufficient filling solution Refill the filling solution
Electrode does not work Replace the failed electrode
Dust or moisture around the electrode or plug Clean and dry the electrode and plug
Tubing loose reconnected
Pump tube stuck recover the tube
Pump tube broken replace the tube
Tubing blockage clean the tubing
Gasket between the electrodes wrong placed or Re assemble it
missing
Leakage between the electrode Reinstalled firmly
Optical coupler too dirty or broken Clean or replace it
Mixing Chamber Blocked Clean Mixing Chamber

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6.6 AB slope and result abnormal
Cause Solution
Mixing Chamber Blocked Clean Mixing Chamber
Mixing chamber leakage Check tubing connection and chamber cover
condenser condensing Liquid outlet blocked
AB slope is zero No Std R, pressure sensor cable loose or damaged.
Mixer dont move Reconnect J111 , replace mixing motor
Liquid outlet valve dont work Reconnect J111 , replace liquid outlet valve
Air outlet dont work, leakage, AB slope is low Reconnect J109, replace air outlet valve
AB slope abnormal Std R invalid, leaked or blocked

6.7 Auto sampler trouble shooting.

Cause Solution

Auto Sampler cannot be detected Check J112 connector and cable, replace Auto sampler motor
The initial position of auto sampler is not on the Check J103 connectors, clean the dust on the optical coupler
clean position for auto sampler home position, replace the optical coupler
Auto sampler on incorrect position Check J104 connector and cable, clean optical coupler,
replace the optical coupler

6.8 Aspiration abnormal

Cause Solution
Aspirating tube loose or broken Connect again or replace it
Pump tube sticks Restore the tube
Pump tube broken Replace the tube
Pump tube blocked Clear the blockage
The gasket between the electrodes does not Place the gasket properly
placed properly or missing
The electrode assembly leaks Tighten the assembly again
Dust in the positioner, or positioner damaged Clean or replace the positioner

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