Service Manual

Auto Hematology Analyzer

Model: KT-6400
 Version：A/1

Intellectual Property
SHENZHEN GENIUS® ELECTRONICS CO.,LTD.

SHENZHEN GENIUS® ELECTRONICS CO.,LTD.(hereinafter called Genius) owns all rights

to this unpublished work and intends to maintain this work as confidential. Genius may also seek
to maintain this work as an unpublished copyright. This publication is to be used solely for the
purposes of reference, maintenance or repair of Genius equipment.

Genius owns this manual and all intellectual property rights(including copyright) in this manual.
No body is allowed to use, disclose or let others obtain part or whole information of this manual in
any manner whatsoever without the written permission of Genius. Nobody is allowed to take the
following actions to part or all contents of this manual, including (but not limited to) shoot,
reproduce, copy, or translate it into other languages without the written permission of Genius.

Genius reserves the final explanation right to this manual.

Genius reserves the right to modify the content of this manual without prior notice.
Genius reserves the right to make technical alterations without prior notice.
Genius reserves the right to change the product specifications without prior notice.

Item No.: P01.91.700031-00

Statement
Regarding this manual, Genius will not take any warranty responsibility, expressed or implied,
including (but not limited to) warranties of merchantability and fitness for any particular purpose.
Genius is responsible for safety, reliability and performance of this equipment only in the
conditions that:

are conducted by Genius authorized personnel;

s used in accordance with the operation guide.

Genius will not take the responsibility for the products’ safety, reliability and performance based on
the following situations:
；
are not properly operated as the Operation Manual.

1
Warranty
Maintenance Service
Free service scope
Where the equipments conform to the provisions of Genius Warranty Services Ordinance
range can enjoy the free service.
Services to be charged
（1）Services not included in Genius Warranty Services Ordinance range should be charged
by Genius company;
（2）Damages caused by the following reasons should be charged for the services, even in
warranty period:
Man-made damage;
Network voltage exceeds the equipment’s specified range;
Irresistible natural disasters.
Genius will not be responsible for the direct, indirect or eventual damages and delay resulting
from the following conditions(including but not limited to): use the instrument improperly, replaced
parts or accessories not approved by Genius or repair the instrument by people other than Genius
authorized personnel.

This instrument must be repaired by Genius authorized personnel.

It is important for the hospital or organization that employs this equipment to carry out a
reasonable maintenance schedule. Neglect of this may result in machine breakdown or injury of
human health.
 Version：A/1

Chapter1 General ................................................................................................................................ 3

1.1 APPLICATION RANGE .................................................................................................................. 3

1.2 MANUAL GUIDE .......................................................................................................................... 3
1.3 COMMON SYMBOLS .................................................................................................................... 4

Chapter2 Hardware ............................................................................................................................. 5

2.1 INTRODUCTION OF CPU BOARD UNIT .......................................................................................... 5

2.1.1 CPU Board Unit Modules’ Connection .................................................................................... 5
2.1.2 Brief Instruction of Unit Modules .......................................................................................... 6
2.1.3 CPU Board Connection Terminals and Instruction ................................................................. 7
2.2 INTRODUCTION OF DRIVER BOARD UNIT ...................................................................................... 9
2.2.1 Driver Board Unit Modules’Connection ................................................................................... 9
2.2.2 Brief Instruction of Unit Modules ............................................................................................. 9
2.2.3 Driver Board Connection Terminals and Instruction ............................................................... 9
2.3 INTRODUCTION OF LCD ADAPTER BOARD UNIT .......................................................................... 11
2.4 INTRODUCTION OF PRINTER ADAPTER BOARD ............................................................................ 12
2.5 HARDWARE DEBUG................................................................................................................... 12
2.5.1 Power Supply Module Detection ........................................................................................ 12
2.5.2 CPU Board Detection ......................................................................................................... 13
2.5.3 Driver Board Detection ....................................................................................................... 13
2.5.4 Display Module Debugging ................................................................................................ 14
2.5.5 Printer Module Debugging ................................................................................................. 14
2.5.6 Microswitch(SS-5-Omron) Debugging ............................................................................... 14

Chapter3 Components Disassembling ........................................................................................... 15

3.1 HOST STRUCTURE .................................................................................................................... 15

3.2 HOST DISASSEMBLING .............................................................................................................. 17
3.2.1 Removal of Left/Right Board and Top Cover ..................................................................... 17
3.2.2 Removal of Back Cover, Power Supply & Its Fixing Plate ................................................. 19
3.2.3 Removal of Front Panel, LCD Screen &Its Adapter Board ................................................ 20
3.2.4 Disassembling of Front Plate’s Fixing Assembly................................................................ 21
3.2.5 Disassembling of CPU Board and Driver Board................................................................. 24
3.2.6 Disassembling of Mechanical Parts’ Fixing Plate Assembly .............................................. 25

Chapter4 Fluidic System .................................................................................................................. 28

4.1 OVERVIEW ............................................................................................................................... 28

4.2 FLUIDIC SYSTEM SCHEMATIC OF THE INSTRUMENT .................................................................... 28
4.3 FUNCTIONS OF THE FLUIDIC SYSTEM ......................................................................................... 28
4.4 COMPONENTS OF FLUIDIC SYSTEM ............................................................................................ 29
4.5 SEQUENTIAL MODULES OF THE FLUIDIC SYSTEM ........................................................................ 29
4.5.1 Lyse Infusion Module .......................................................................................................... 29
4.5.2 Counting Module ................................................................................................................ 30
4.5.3 Cleaning Module ................................................................................................................. 30
4.5.3.1 Counting Chambers Cleaning .................................................................................................. 30

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 4.5.3.2 Sample Probe Cleaning............................................................................................................ 32
4.5.4 Mixing Module .................................................................................................................... 32
4.5.5 Waste Discharging Module................................................................................................. 33
4.6 OVERVIEW OF COUNTING PROCESS .......................................................................................... 33
4.6.1 Venous and Capillary Mode ............................................................................................... 33
4.6.2 Prediluted Mode .................................................................................................................... 34
4.6.3 Hibernation and Wake Up .................................................................................................. 35

Chapter5 Histograms ........................................................................................................................ 36

5.1 OVERVIEW ............................................................................................................................... 36

5.2 NORMAL HISTOGRAMS .............................................................................................................. 36
5.2.1 Neutrophilic Leukocytosis .................................................................................................. 36
5.2.2 Lymphocytosis .................................................................................................................... 36
5.2.3 Monocytosis ....................................................................................................................... 37
5.2.4 Tumor Patients ................................................................................................................... 37
5.3 ABNORMAL HISTOGRAM ............................................................................................................ 37
5.3.1 Unclassified White Blood Cells .......................................................................................... 37
5.3.2 Increased Nucleated Erythrocytes or Interference or Inadequate Hemolysis.................... 38
5.3.3 Severe Interference in WBC Channel ................................................................................ 38
5.3.4 No Lyse or Poor Hemolysis ................................................................................................ 38

Chapter6 Troubleshooting ............................................................................................................... 39

6.1 OVERVIEW ............................................................................................................................... 39

6.2 INSTRUCTIONS FOR TROUBLESHOOTING .................................................................................... 39
6.3 FAULTS CAUSED BY EXTERNAL FACTORS .................................................................................. 40
6.3.1 Reagent Factors ................................................................................................................... 40
6.3.2 External Interference ............................................................................................................. 40
6.3.3 Sample Factor ....................................................................................................................... 40
6.3.4 Instrument Settings ............................................................................................................... 41
6.4 INTERNAL FAULTS OF THE INSTRUMENT ..................................................................................... 41
6.4.1 Faults Detection ................................................................................................................. 41
6.4.2 Gain Adjustment ................................................................................................................. 43
6.5 INSTRUMENT SPECIAL ............................................................................................................... 45
6.5.1 Factory Calibration ............................................................................................................. 45
6.5.2 System Settings ................................................................................................................. 46
6.5.3 Continuous Tests ............................................................................................................... 47
6.5.4 All Pulse Graphic ................................................................................................................ 48
6.5.5 History Query ..................................................................................................................... 49
6.5.6 Version Setting ................................................................................................................... 50
6.5.7 Measured Times ................................................................................................................. 50
6.5.8 System Adjustment ............................................................................................................ 51
6.5.9 Parameters Setting ............................................................................................................. 51
6.5.10 Vent Diluent ...................................................................................................................... 52
6.6 COMMON FAULTS AND SOLUTIONS ................................................................................................ 52

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 Chapter1 General
This chapter explains how to use your KT-6400 service manual, which provides detailed
reference information and procedures needed for servicing your KT-6400 analyzer. Thoroughly
read this manual and understand its meaning before servicing your analyzer, only by this, you can
do the maintenance properly and ensure the safety of service personnel.
This manual is to be used in conjunction with the KT-6400 analyzer operation manual and
does not contain information and procedures already covered in the operation manual.

Be sure to service your analyzer strictly as instructions in this manual and operation manual.

1.1 Application Range

This service manual is written for people who

have a thorough understanding of electronic and fluidic principles and can make
corresponding analysis;
have a thorough understanding of reagent systems;
have a thorough understanding of quality control;
have a thorough understanding of troubleshooting concepts;
have an operator’s knowledge of the analyzer;
ve the ability to use basic mechanical tools and understand related terminology;
have the ability to use a digital voltmeter (DVM) and an oscilloscope.

1.2 Manual Guide

This service manual contains 6 chapters. Users can search for the related chapter according
to their needs:

If you want to… See…

learn about the hardware and how to test the
Chapter 2 Hardware
boards of KT-6400
learn about the mechanical structure and how to Chapter 3
disassemble parts and components of KT-6400 Components Disassembling
learn about the fluidic system functions Chapter 4 Fluidic System

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 learn about the faults reflected by histograms and
Chapter 5 Histograms
pulse graphs
learn about how to troubleshoot your KT-6400 Chapter 6 Troubleshooting

1.3 Common Symbols

Symbols used in this service manual and their meanings:

Symbols Meaning
Alerts the operator to follow the statement below
the symbol while in operation, otherwise it may
cause personal injury.
Alerts the operator to follow the statement below
the symbol, otherwise it may lead to products’
faulty, damage or unreliable analysis results.
Alerts the operator to follow the statement below
the symbol, which emphasizes the important
information or special attention to be paid while in
operation.
Alerts the operator to follow the statement below
the symbol. Otherwise it takes the risk of potential
biohazard.

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 Version：A/1

Chapter2 Hardware
In order to let users and maintenance personnel understand relevant knowledge of the
hardware, namely the connection of relevant circuit modules. Below is an introduction for the host
structure and circuit unit modules:
Connection schematic of all unit modules inside the host, the host mainly consists of CPU
board, driver board, LCD adapter board, printer adapter board, power supply and LCD assembly
and so on.

2.1 Introduction of CPU Board Unit

2.1.1 CPU Board Unit Modules’ Connection

Fig. 2-1 Schematic of CPU board’s functional modules

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2.1.2 Brief Instruction of Unit Modules

1）CPU（OK6410 core board）

Control the whole system’s running, dispatch tasks for each interfaces and receive HGB,
WBC, RBC, PLT data from the FPGA.
2）CF card interface, internet access, SD card interface
CF card is used to store part of the programs, help to extend the Core board’s memory
space. Internet access circuit adopts the DAVICOM ’s DM9000A Ethernet control IC, which
supports the IEEE802.3u and 802.3 x transport protocols and connects with Ethernet network. SD
card interface externally connects with SD card to send or read data.
3）Serial interface circuit
J16 serial port is used to communicate with external-connected PC, J18 serial port is used to
communicate with the inner printer adapter board, J21 is used to communicate with driver board
and transmit the controlling data package to the motors and valves.
4）4 USB ports and 2 PS2 interface
UPD720114 controls 4 USB ports’data sending and receiving, MIC2526-2BM provides power
management, J24, J25 USB ports are extended to the front surface of the shell through USB
adapter cables. J26, J27 USB ports are welded on the CPU board.
PS2 interface usually adopts PCA9306 to control the data’s receiving and sending, these
two PS2 interfaces are used to connect with mouse and keyboard.
5）LCD interface, VGA interface circuit
Use the three 10 video D/A of SDA7123 to convert IC, then transit the data to LCD adapter
board through the 40 pin soft ribbon cable. After the processing of THC63LVDF63A, the data is
displayed on the LCD screen through LCD cable. While VGA interface circuit is used for an
external monitor.
6）FPGA control circuit
Extract HGB, WBC, RBC, PLT data from AD converting chip, then transmit them to the core
board.
7）WBC, RBC channels and WBC, RBC gain adjustment.
Magnify the detected WBC, RBC signals to be easily recognized and transformed into
corresponding digital signals and make the gain adjustment to them through digital potentiometer.
8）HGB channel and HGB gain adjustment
Magnify the detected HGB signals to be easily recognized and transformed into
corresponding digital signals and make the gain adjustment to them through digital potentiometer.
9）Voltage monitoring circuit
Monitor ±12V and constant current source voltage
10）Two ignition circuits and constant current source circuit

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 Ignition circuit is used to cauterize and unclog the apertures with a voltage of 110v ignition
voltage. Constant current source is mainly composed of two transistor (2SA970) and the 60v is
used as a constant input voltage to generate constant current sent to the signal terminal. When the
signal end (i.e. inner side of measuring cup) gets particles pass, the resistance will change while
the current constant, so the voltage will also change, this changed voltage is the voltage we need,
namely signal current.

2.1.3 CPU Board Connection Terminals and Instruction

A. Schematic of the connection terminals

Fig. 2-2 Schematic of CPU board connection terminals

B. Instruction
1）Connecting port for external display screen
CON1：Used to connect with external display screen
2) Communicating port for external PC
J16：Communicating port for external PC
3) USB port
J25、J24：USB sockets, transferred to front panel through USB cables.
J26、J27：USB ports
4) PS2 port
J36: PS2 port, connected to external mouse

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 J37: PS2 port, connected to external keypad
5) Internet access
U53: Ethernet network interface
6) Serial port
J18: Connected with JP3 on the printer adapter board, transmitting the printing data package
to the printer adapter board’s processor to print through the inner printer.
J21: Connected with J13 on driver board, transmitting the motors and valves controlling data
packages into the driver board’s processor to be processed.
7) Test microswitch
J19: Microswitch terminal for sampling test, touch the microswitch, the sampling needle
aspirate blood to test.
J23: Microswitch terminal for door open alarm
8) FPGA program download port
J7：Download program into FPGA
9) WBC/RBC/HGB signal input
J34:WBC signal input
J33:RBC signal input
J9:HGB signal input
10) Power input
J35:+110V voltage input terminal
J13:±12V voltage input terminal
J12:+5V voltage input terminal
11) LCD screen interface
J11: Connected with J1 on LCD adapter board
12) CF card port
J15: CF card socket
13)SD card port
SD: SD card socket
14) Reserved ports
J5, J6, J17, J20, J22, J28, J40 are reserved ports, not be processed.

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2.2 Introduction of Driver Board Unit

2.2.1 Driver Board Unit Modules’Connection

Fig 2-3 Schematic of driver board’s functional modules

2.2.2 Brief Instruction of Unit Modules

1）ARM control circuit

Through the serial port to extract controlling data package from the CPU board, then transmits
the data to FPGA and FPGA will execute the driving commands to motors and valves.
2）Serial port receives data from CPU board
The CPU board transmits the motors and valves’controlling data package to the driver board
by serial port communication.
3）FPGA control circuit
The drive motors and valves extract the sensor’s signal to control their motions.

2.2.3 Driver Board Connection Terminals and Instruction

A. Schematic of connection terminals

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 Fig 2-4 Schematic of driver board’s connection terminals
B. Instruction
1) Signal input port of the sensor
J1: sensor1-6，only three of them are used (four lines for each way), negative pressure pump
sensor, diluent sensor and lyse sensor, respectively connected with the corresponding sensors on
the mechanical parts’ fixing plate assembly.
J2: sensor7-12，only five of them are used (four lines for each way), X left sensor, X right
sensor, Y up sensor, Y down sensor, aspirating pump sensor, respectively connected with the
corresponding sensors on the mechanical parts’ fixing plate assembly.
2) Valve drive port
J16: valve drive port, valve 1-12 (SV1 - SV12) respectively connected with SV1-SV12 on the
mechanical parts’ fixing plate assembly.
3) Motor driver ports
MOTO4: Vertical motor driver port, connected with the corresponding motor on the
mechanical parts’ fixing plate assembly.
MOTO3: Horizontal motor driver port, connected with the corresponding motor on the
mechanical parts’ fixing plate assembly.
MOTO2: Aspirating pump motor driver port, connected with the corresponding motor on the
mechanical parts’ fixing plate assembly.

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 MOTO1: Negative pressure pump motor driver port, connected with the corresponding motor
on the mechanical parts’ fixing plate assembly.
4) ARM download end
J12: Download programs into ARM
5) FPGA download end
J9: Download programs into FPGA
J8: FPGA debugging interface
6) Serial port communication terminal
J13: receive the motors and valves controlling data packages from the CPU board’s serial port.
7) Input terminal of waste liquid sensor
J20: directly connected to the corresponding cable terminal on the mechanical parts’ fixing
plate assembly.
8) Power input
J11: +5/+12V voltage input terminal
J10: +24V voltage input terminal
9) Reserved ports
J3, J4, J19, MOTO5 are reserved terminals

2.3 Introduction of LCD Adapter Board Unit

Fig. 2-2

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J1: LCD adapter board communicate with the CPU board J11 through J1 to extract video data.
After being processed by THC63LVDF63A chips, displayed on the screen through J5 port.
J3: connected with the high-voltage slat’s J2 to control the display backlighting.
J4: connected with the touch screen data cable to receive the touch screen data. After being
processed by THC63LVDF63A chip, transmitted to the CPU through J1.
J2：+12V voltage input terminal

2.4 Introduction of Printer Adapter Board

Fig. 2-3
JP1：+5V/+12V voltage input terminals
JP2: printer soft ribbon cable socket, the other end is plugged into the socket of soft ribbon cable
adapter board.
JP3: serial port communicating with CPU board, to receive the printing data packages from CPU
board.
J1: single chip microcomputer program interface on the printer adapter board.

2.5 Hardware Debug

2.5.1 Power Supply Module Detection

The power supply model of KT - 6400 is: FYH192M12E. To determine whether the power
failure, the power supply’s no-load output should be detected. The detecting methods as follows:
1). Resistance detection with power on：①Use a multimeter to measure the resistance
between the switch power +12 V and the ground, usually between 490-510 ohms; ②Use a
multimeter to measure the resistance between the switch power -12 V and the ground, usually

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between 470-490 ohms;③Use a multimeter to measure the resistance between the switch power
+5 V and the ground, usually between 90-110 ohms;④Use a multimeter to measure the resistance
between the switch power +24 V and the ground, usually between 700-900 ohms;⑤Use a
multimeter to measure the resistance between the switch power +110 V and the ground, usually
between 80K-100K ohms;when the resistance is zero, power failure.
2). Short circuit detection before power on: use a multimeter to measure wether short circuit
or not between the power supply +5 V and the ground, +12 V and the ground, -12V and the ground,
+24 V and the ground, +110 V and the ground, if short out, then power failure.
3). Detection of the power supply’s no-load output voltage while power on
While no problems found in 1) and 2), disconnect the plugs between the power and the main
board, driver board, LCD adapter board, printer adapter board, then power on the switch and use a
multimeter to measure its output voltage. The corresponding output voltages’ error range are:
+5 V： +4.75V ～ +5.25V
+12V：+11.75V ～ +12.25V
-12 V：-11.75V ～ -12.25V
24 V: +22.75V ～ +24.25V
110 V: +109.75V ～ +110.25V

2.5.2 CPU Board Detection

Power on the CPU board, measure the voltage on the board normal or not (voltage test points
on the board include: +12 V,-12V, +5 V,-5V, +3.3 V, +1.2 V, 2.5V, GND, PGND, AGND),
measuring method as follows: (To measure in accordance with the corresponding relationship
between the voltage and ground while in short circuit tests)
Corresponding voltage’s error range for each voltage test point:
Voltage test points Correct measurement range
+12V +11.85V ~ +12.30V
-12V -12.30V ~ -11.85V
2.5V 2.4V ~ 2.6V
+5V +4.95V ~ +5.15V
+3.3V +3.29V ~ +3.35V
+1.2V +1.18V ~ +1.30V

2.5.3 Driver Board Detection

Power on the driver board, use a multimeter to measure the voltage on the board normal or
not (voltage test points on the board include: +12 V,+5 V, +3.3 V, +1.2 V, +2.5V,+24V, GND,

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PGND, AGND), measuring method as follows: (To measure in accordance with the corresponding
relationship between the voltage and ground while in short circuit tests)
Corresponding voltage’s error range for each voltage test point:

Voltage test points Correct measurement range

+12V +11.85V ~ +12.30V
+24V +23.5V ~ +24.5V
+2.5V +2.4V ~ +2.6V
+5V +4.95V ~ +5.15V
+3.3V +3.29V ~ +3.35V
+1.2V +1.18V ~ +1.30V

2.5.4 Display Module Debugging

Check whether the screen and CPU board are well connected or not, and any situations like
virtual welding, missed welding, short circuit, wrong welding, etc. on the revealed circuit part of
PCB board.

2.5.5 Printer Module Debugging

Connect the printer, display screen with CPU board well. Turn on the power without print
paper to observe whether report “No paper inside the printer”, if error reported, it’s normal. Put on
the paper, then turn on the power to observe whether the printer rotates, if rotates, it’s normal.
Detect if foot 1 of Q1 chip is 8 V, for around 8 V, it’s normal; remove the print paper, then detect
whether foot 1 of U3 chip is high or not (around 3.3 V), if high, it’s normal.

2.5.6 Microswitch(SS-5-Omron) Debugging

Place well the properly-connected microswitch with socket, use a multimeter to measure the
2 pin on the socket: while switch not pressed down, it’s open circuit state, while pressed down it is
in short circuit state, otherwise it is abnormal; Plug the microswitch into J19 on the CPU board,
then turn on the power. Use a multimeter to measure J19 foot 1 voltage, while not pressed down, it
is high level (3.3 V), while pressed down, it is low level (0 V), otherwise abnormal.

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 Version：A/1

Chapter3 Components Disassembling

3.1 Host Structure

Fig. 3-1 Front view of the host

Fig. 3-2 Front view with front panel open

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 Fig. 3-3 Back view of the host

Fig. 3-4 Left-side view with door open

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1-- Sampling Pump 2-- Negative Pressure Pump 3-- Solenoid Valve 4-- Right Board
Fig. 3-5 Right-side view with door open

3.2 Host Disassembling

Disconnect the instrument with power supply before trying to assemble/disassemble your
analyzer or do the maintenance.
The surfaces of all the analyzer components are potentially infectious, properly protective
measures should be taken before the operation or maintenance.
Use the specified tools and spare parts to service the instrument, when the work’s done, clean
the used tools according to the tools’ use instructions.

3.2.1 Removal of Left/Right Board and Top Cover

1). Removal of right board

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 Fig 3-6 Removal of the right board
As shown in figure 3- 6，the right board is fixed on the backboard through a hinge. So, while in
disassembling, use M3 cross screwdriver to slowly remove the screws. In case of damaging the
hinge, please pay attention to the balance of right board.
2). Removal of left board

Fig. 3-7 Removal of the left board

As shown in figure 3- 7，the left board, namely the lateral board at the circuit boards side. The
disassembling method as follows: use M3 cross screwdriver to remove the three screws fixing the
lateral board, then push the board backward to remove it.
3). Removal of the top cover

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 Fig 3-8 Removal of the top cover
As shown in figure 3- 8，use M3 cross screwdriver to remove the three PM3*6 screws, then
push the top cover backward to remove it.

3.2.2 Removal of Back Cover, Power Supply & Its Fixing Plate

1). Removal of the back cover

Fig 3-9 Removal of the back cover

The back cover is fixed on the fluidic baffle and circuit baffle. While in disassembling, use M3
cross screwdriver to remove the six PM3*6 screws on the back cover, pay attention to the balance
of back cover.

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2). Removal of the power supply

Fig. 3-10 Removal of the power supply

There are four screw holes on the power supply lateral side. And the power supply is fixed on
the back cover through these four screw holes. While in disassembling, just remove the four PM3 *
6 screws on the back cover.

3.2.3 Removal of Front Panel, LCD Screen &Its Adapter Board

1). Removal of the front panel

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 Fig. 3-11 Removal of the front panel
Front panel of the machine is fixed by bolts which fix the door hinge active plate to the door
hinge fixed socket. While in disassembling, just use a pliers to remove E washer and pull out the
bolt, then the front panel can be taken down. Pay attention to the balance of the front panel while in
push, in case of damaging the panel’s internal components.
2). Removal of the LCD Unit Assembly

Fig. 3-12 Removal of the LCD Unit Assembly

As shown in figure 3- 12, the display and display fixing cover are assembled together to form
the display assembly, then fixed on the front panel of the machine. While in disassembling, remove
the display assembly from the front panel first, use the M3 cross screwdriver to loose the six PM3
*6 cross pan head screws on the back of display fixing cover, then remove the display assembly.
After that, loose the 4 PM3 * 6 cross pan head screws fixed on the display using the M3 cross
screwdriver, then take out the display screen.

3.2.4 Disassembling of Front Plate’s Fixing Assembly

To disassemble WBC and RBC measuring cup assembly, use M3 cross screwdriver to loose
the fixing screws on the shielding box and take the box out. Then use M3 cross screwdriver to take
down the measuring cup’s fixing plate assembly from the mechanical part’s fixing plate. While in
loosing the screws, please take care the insulating spacer between the fixing hole of measuring
cup’s fixing plate and the mechanical part’s fixing plate. This insulating spacer is used to isolate

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the measuring cup’s fixing plate from mechanical part’s fixing plate. When the screws are loosed,
please take care to prevent the insulating spacer falling down. Parts scattergram of measuring cup
assembly as Fig. 3-13, Fig. 3-14.

Fig.3-13 Parts scattergram of WBC measuring cup assembly

Fig. 3-14 Parts scattergram of RBC measuring cup assembly

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 Disassemble the sampling assembly. Loose the three screws on the sampling baseplate, the
sampling assembly can be taken down. Parts scattergram of the sampling assembly shown in
Figure 3- 15.

Fig. 3-15 Disassembling diagram of the sampling assembly

Disassembling of the deflection assembly. Remove the bottom deflection motor first before
disassembling the deflection assembly. Method as follows: first loose the jackscrew on the lower
deflection plate which connects the motor with the deflection assembly; then loose the four motor
fixing screws on the sampling - lower plate, then the motor can be removed and the deflection
assembly can be taken down at the same time.

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 Fig. 3-16 Removal of sampling probe

Removal of sampling probe

Loose the locknuts on the puncture needle, as shown in figure 3-16, pull up the sampling
needle vertically to remove the sampling needle.

3.2.5 Disassembling of CPU Board and Driver Board

Fig. 3-17 Disassembling of CPU board and driver board

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 The CPU board and driver board are fixed on circuit boards’ fixing lateral plate. While in
disassembling, unplug the connecting cables between the CPU board and driver board first, loose
the corresponding screws fixing CPU on the lateral plate; while disassembling the driver board,
just use M3 cross screwdriver to loose its fixing screws.

3.2.6 Disassembling of Mechanical Parts’ Fixing Plate Assembly

1). Disassembling of whole mechanical parts’ fixing plate assembly

Fig. 3-18 Disassembling of whole mechanical parts’ fixing plate assembly

As shown in figure 3- 18: the fluidic system baffle consists of valve components, sample
aspirating pump components and negative pressure pump components. While in disassembling,
just use a cross screwdriver to loose the corresponding PM3 * 6 pan head cross screws, then use
your hands to hold the mechanical parts’ fixing plate assembly and gently move it away.
2). Disassembling of sample aspirating pump
Sample aspirating pump assembly is fixed on the mechanical parts’ fixing plate and the
aspirating piston bracket by four PM3 * 30 pan head screws. While in disassembling, use M3 cross
screwdriver to loose screws on mechanical parts’ fixing plate, then it can be removed.
3). Disassembling of the solenoid valve components. Solenoid valve components are fixed on the
mechanical parts’ fixing plate by PM3 *6 pan head screws coupled with resilient pad and flat
gasket. While in disassembling, use M3 cross screwdriver to loose the screws, then the solenoid

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valve assembly can be taken down from the mechanical parts’ fixing plate. Solenoid valve bracket
parts scattergram as below figure 3- 19:

Fig. 3-19 Disassembling of solenoid valve components

4). Disassembling of negative pressure pump assembly and motor system

Fig. 3-20 Disassembling of negative pressure pump motor system

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 As shown in figure 3- 20, first use M3 cross screwdriver to loose the four PM3*6 motor fixing

cross pan head screws on the upper plate of negative pressure pump, then remove the coupler’s

two screws to take down the motor; Remove the corresponding screws, then the components are

disassembled.

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 Version：A/1

Chapter4 Fluidic System

4.1 Overview

This chapter intends to introduce the fluidic system of KT-6400, so the users or service
personnel can acquire relative information of the fluidic system.

4.2 Fluidic System Schematic of The Instrument

Fluidic system schematic of the instrument as follows:

Fig. 4-1 Fluidic system schematic of the instrument

4.3 Functions of the Fluidic System

1). To prepare diluent for the whole blood mode and prediluted mode;
2). Count the blood cells and measure HGB;
3). Release the precise amount of diluent;
4). Auto Cleaning;

28
5). Control the pressure and vacuum

4.4 Components of Fluidic System

The fluidic system consists of the following subsystems: sensor subsystem, lyse dispensing
and mixing subsystem, diluting subsystem, vacuum/pressure subsystem and auxiliary subsystem,
etc..
The key components of the fluidic system are the solenoid valves, gem apertures, diluent
dispenser, isolated chamber, pump, counting chamber, negative/positive pressure chamber and
rubber tubes, etc..

4.5 Sequential Modules of the Fluidic System

The fluidic system can be divided into the following functional modules: counting module,
washing module, mixing module and waste discharging module, etc.. This section will introduce
you each sequential module’s function in the fluidic system.

4.5.1 Lyse Infusion Module

Infuse lyse, as shown in fig. 4-2:

Fig. 4-2 Lyse infusion module

When the piston of sampling pump moves down, NO side of valve V1 will be open, and NC
side of valve V1 will be closed to aspirate lyse; When the piston moves upward, NO side of valve
V1 will be closed and NC side open to infuse lyse into the counting chamber.

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4.5.2 Counting Module

Single channel fluidic system’s counting module structure as figure 4-3, consists of counting
chamber, valve 5, valve 7, measuring cup, volume measuring module, negative pressure pump
and relevant support system.

Fig. 4-3 counting module

The counting module completes the most important function of the analyzer - counting. The
electrodes installed on the counting chamber detect the cell pulses when they pass the aperture by
Coulter Principle. The detected pulses are then transmitted to the analog circuit to be amplified,
rectified, recognized, threshold adjusted and finally count the cells.
When the sample is mixed well in the counting chamber, the machine establishes the
vacuum and open valve 9. Sample (blood cells) in the chamber is lead to cross the aperture under
the negative pressure to generate pulses and finish the counting process.

4.5.3 Cleaning Module

Cleaning module of the fluidic system includes: counting chamber, back chamber; sampling
probe’s cleaning, etc..

4.5.3.1 Counting Chambers Cleaning

Cleaning module structure as fig. 4-4, when the instrument gives cleaning command to the
counting chamber, the cleaning process starts.

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 Fig. 4-4 Cleaning module of fluidic system
1). Cleaning of the front counting chamber: When the piston of sampling pump moves down,
NO side of valve V2 will be open and NC side closed to aspirate diluent. When the piston moves
upward, NO side of valve V2 will be closed and NC side open to prime diluent, with Valves V3 and
V4 NO sides open and NC sides closed, the diluent will be infused into the WBC counting chamber
when NO side of V5 open while NC side closed, when V5 NO side closed while NC side open, the
diluent will be infused into the RBC counting chamber. Open Valves V8 and V10, close valve V12
and pull the syringe of negative pressure pump up, the waste liquid will be aspirated into the
negative pressure pump.
2). Back chamber cleaning.

Fig. 4-5 Back chamber cleaning

As shown in Fig. 4-5, When valve V2 No side open, NC side closed and Valve V6 closed, the
syringe of the sample aspirating pump is pulled down to aspirate diluent. Then open valve V6 and

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pull up the syringe to infuse the diluent into the back chamber. Then open valve V9, the negative
pressure pump syringe is pulled up to aspirate the waste liquid into the negative pressure pump.

4.5.3.2 Sample Probe Cleaning

Fig. 4-6 Sample probe cleaning

With valve V2 NO side open and NC side closed, the syringe of sample aspirating pump is
pulled down to aspirate diluent. When the syringe of sample aspirating pump is pushed up, NC
side of valve V2 open, NO side closed, while valve V3 NO side open, NC side closed to infuse
diluent into the swab with valve V4 NC side open and NO side closed. At the same time, valve V11
is opened and the syringe of negative pressure pump is pulled up to aspirate the waste liquid into
the negative pressure chamber and complete the cleaning to the sample probe’s outer wall. When
the sample aspirating pump moves up, NC side of valve V3 open and NO side close to infuse
diluent into the syringe. Then take the pressure of the diluent syringe to push the diluent out of the
sample probe and complete the cleaning to the sample probe inner side.

4.5.4 Mixing Module

Bubble mixing method is applied here. As shown in figure 4-7, before the cells counting, the
samples need be diluted. Once the samples are infused into the measuring cup and added with a
certain amount of diluent, the system will inform the mixing module to work. At this moment,
positive pressure is established inside the negative pressure pump, through brief on/off of valve V8,
V10, several air segments are formed inside the pressure chamber and injected into the
measuring cup from its bottom and generate the bubbles inside the cup. The bubbles pop up to the
liquid level from the cup’s bottom, which causes the liquid to slightly writhe, thus the mixing is
completed.

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 Valves’ on/off interval control is critical to take this bubble mixing method. Either too many or
too few bubbles will affect the mixing effect. While generating the bubbles, the airway should be
well drained without liquid accumulation, otherwise it will affect the bubbles quantity as well as the
dilution ratio.

Fig. 4-7 Mixing module

4.5.5 Waste Discharging Module

Waste discharging module includes: counting chamber and pressure chamber’s waste liquid
discharging. As shown in figure 4-7, when the piston of the negative pressure moves up to
establish negative pressure, valves V8 and V10 will be open and V12 close to aspirate the cups’
liquid into the pump; when the piston of the negative pressure pump moves down to establish
positive pressure, valves V8, V10 will be closed and V12 open to discharge the pump’s waste
liquid.

4.6 Overview of Counting Process

For the usual testing blood samples, all the cells are interlapped to each other, so the
analyzer can not precisely count the blood cells or calculate their volume distribution. For this
reason, samples should be diluted before the counting or calculating the volume distribution. This
analyzer has the second dilution function, under the whole blood sample and prediluted blood
sample modes, the analyzer’s second dilution ratio are different, introduction as follows:

4.6.1 Venous and Capillary Mode

As fig. 4-8, under the venous blood and capillary blood mode, the operator can directly sent

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the samples to test. The machine will aspirate 10μL sample blood and mix it with 2.49ml diluent to
form a dilution ratio of 1:250 diluted sample. Then take out 30μL diluted sample, add 0.35ml lyse
into the remaining diluted sample and form a 1: 285 cell suspension to measure the WBC and
HGB. Then inject the aspirated 30μL diluted sample into RBC chamber, add 2.57ml diluent to form
a dilution ratio of 1:21667 RBC diluted sample for the counting of RBC and PLT and generate
corresponding histograms.

Fig. 4-8

4.6.2 Prediluted Mode

As figure 4-9, under the prediluted mode, the operator should first mix the 20μL capillary
sample with 1000μL diluent to form a 1:51 diluted sample outside the machine. Then serve the
pre-diluted sample to the sample probe, the instrument will aspirate 320μL diluted sample and mix
it with 2.0ml diluent to form a dilution ratio of 1:370 diluted sample. Then take 30μL diluted sample
out of the sample, add 0.35ml lyse to the remaining diluted sample to form 1:816 cells suspension
and measure the WBC and HGB. Then inject the aspirated 30μL diluted sample into the RBC
chamber, add 2.57ml diluent to form a dilution ratio of 1:32045 RBC diluted sample for the
counting of RBC and PLT and generate the corresponding histograms.

- 34 -
 Fig. 4-9

4.6.3 Hibernation and Wake Up

Long time no operation on the instrument, it will automatically enter into dormant state.
Parameters can be adjusted to control the instrument’s dormant state and auto Sleep Time
through the control button under the setting interface of main menu:

Fig. 4-1
After 30 minutes’ hibernation, the system will automatically take back the sample needle. For
the next 30 minutes hibernation, the system will automatically run the cleaning program. If the
hibernation time is over 1 hour, the system will automatically prim for the whole machine. To wake
up the instrument, just press the sampling switch, or click the mouse’s left button or touch the
screen, then the hibernation is canceled.

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 Version：A/1

Chapter5 Histograms

5.1 Overview

This chapter demonstrates some possible WBC histograms in practical operation for the
users and service personnels to do relative faults diagnosis.

5.2 Normal Histograms

Fig. 5-1
Note: Blood cells lain between the first and the second discriminators are
lymphocytes; those between the second and the third discriminators are mid-sized cells;
those between the third and the fourth discriminators are neutrophil The fourth
discriminator is the fixed line.

5.2.1 Neutrophilic Leukocytosis

Fig. 5-2

5.2.2 Lymphocytosis

Fig. 5-3

36
 5.2.3 Monocytosis

Fig. 5-4

5.2.4 Tumor Patients

Fig. 5-5

5.3 Abnormal Histogram

5.3.1 Unclassified White Blood Cells

Fig. 5-6
Could be caused by the WBC gain setting error.

When the count is less than a certain value (WBC < 0.5), the instrument will not classify
WBC on the histogram.

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5.3.2 Increased Nucleated Erythrocytes or Interference or Inadequate Hemolysis.

Fig. 5-7

5.3.3 Severe Interference in WBC Channel

Fig. 5-8

5.3.4 No Lyse or Poor Hemolysis

Fig. 5-9

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 Version：A/1

Chapter6 Troubleshooting

6.1 Overview

This chapter introduces the displayed fault messages, possible causes and suggested
solutions. If the error remains after trying the recommended method, please check the hardware
and try to replace the fittings or PCB boards which are suspected of causing the error.

6.2 Instructions for Troubleshooting

1). Disconnect the power cord with the analyzer before disassembling your analyzer.
2). In case of leakage inside the analyzer, be sure to turn off the analyzer immediately and
wipe off the liquid, otherwise it may cause personnel injury or property damage.
3). Be sure to keep your hair, clothes, cuffs or hands away from the moving parts of the
analyzer.
4). Be sure to use specified tools or parts to maintain this analyzer and be sure to clean the
used tools as instructed by their use manual after the maintenance is finished.
5). Be sure to use soft and clean cloth, neutral detergent-soaked cloth (twisted dry), or soft
cloth washed by ethanol to clean the surface of this analyzer.
6). Be sure to pay attention to the marks or symbols on this analyzer. Be sure not to touch the
power socket at the back of this analyzer with wet hands or wet rags.
7). Be sure not to use corrosive solvent to wash the surface of this analyzer. Otherwise, it may
lead to surface corroded or color faded.
8). Be sure to avoid direct contact with the reagents. Operatives should wear Lab protective
clothing, gloves well and comply with the Lab Safety Operation Regulations. In case of contacting
with the reagents accidentally, be sure to wash them away immediately with clean water. If serious,
go to see a doctor immediately.

The surfaces of all the analyzer components are potentially infectious, proper protective
measures should be taken before the operation or maintenance.
Samples, quality control, calibrators, waste liquids, etc. should be redeemed to have potential
risk of biological infection, so while in contact with the related items, operatives should comply with
the Lab Safety Operation Regulations to wear personal protective equipment well(such as
laboratory protective clothing, gloves, etc.).

39
6.3 Faults Caused by External Factors

6.3.1 Reagent Factors

KT-6400 uses Diluent, Lyse as the reagents, the common faults were reagents deteriorated,
replace the reagents will be OK. The following tips should be noted while in use of the reagents:
 Avoid direct sunshine;
 Do not use frozen reagents;
 The reagents should be placed at the same level with the instrument;
 Do not connect the reagents tubes mistakenly and pay attention if the reagents are used
properly;
 Do not contaminate the reagents, do not pour the remaining reagent into the new reagents.

6.3.2 External Interference

There are a lot of interference source, all the interference may lead to unprecise test, please
refer to the following precautious:
 The instrument should avoid direct sunshine to guarantee its performance.
 Instruments should be kept far away from the CT, MRI, X-ray machines and other high-power
electrical equipments, away from the centrifuge, electric bed, electric power, etc. equipped
with high power motors inside, away from electrical equipments starting with a strong electric
current and carrying big interference. Do not use mobile phones, wireless phones, etc. near
the instrument while in testing, which produce a strong radiation field.
 Equipotential ground terminal on rear panel of the instrument must be connected with the
ground lead, it’s better to use the dedicated ground lead.
 Frequent power interruption will lead to instrument’s serious degeneration on performance
and reliability, so it should be equipped with uninterrupted power supply (UPS).
 While installing the pipelines, they should be kept in a natural relaxed state, can not be forced
to twist, bend and rotate.

6.3.3 Sample Factor

Sample means sample blood which can be tested by the test machine. Sample factor may not
be the sample’s degeneration, should note that:

- 40 -
 The sample blood collection should strictly follow the peripheral blood collection standard of
National Health Department and this field. Note at the same time: while in collecting the blood,
if the blood does not flow well, slight pressure could be forced distally to the would, prohibiting
to exert strength around the puncture area, in case of flowing in the tissue fluid and affecting
the test results.
 While in adding the diluent, the sample tube should be inclined under the probe to let the
diluent flow down through the tube wall, in case of generating bubbles.
 If the blood sample has been placed for a long time and not blended well, it will lead to
measurement error and unprecise test results.
 The testing sample can only be stored at room temperature and should be tested within 4
hours. Do not shake the testing samples violently.

Under any circumstances, do not contact the blood samples, quality controls and calibrators
directly. Should comply with the Lab or Clinical Operation Regulations while disposing these
materials.

6.3.4 Instrument Settings

Improper corresponding settings will lead to abnormal tests, so the following tips should be
noted:
 While doing the Blank test, the instrument only tests and displays WBC, RBC, HGB, HCT,
PLT. Note at the same time: No. 999 is a dedicated number for blank test, do not input this No.
while testing the blood sample.
 While using quality control to calibrate: the dedicated quality control should be used,
otherwise it may cause the deviation of the quality control results. Note at the same time: do
not use bad, outdated quality control products, complete quality control in a fixed time every
day.

6.4 Internal faults of The Instrument

Exclude above factors, if the fault is still existed or error reported, please refer to the
following methods to test or debug the instrument:

6.4.1 Faults Detection

When the instrument reports motor fault, printer fault, or other cases suspected of motor,
solenoid valve or printer faults, please refer to this section to estimate the faults: under the main

- 41 -
menu, click "Service" button to enter into the service menu, then choose "Detection" to enter into
the following interface:

Fig. 6-1
 Solenoid Valve Detection
Click the “T”button on the right to control the valve’s on and off. If "ON" is momentarily
displayed on the corresponding box, the control part of solenoid valve is normal and you will hear
the opening sound of the valve at the same time.
 Motors detection
Motor A: correspond to sample needle’s horizontal movement
Motor B: correspond to sample needle’s up and down movement
Motor C: correspond to sample aspirating pump movement
Motor D: correspond to negative pressure pump movement
Click the corresponding“T”button on motor’s right side to test the motor and reset it. Before
testing motor A, make sure sample probe at the vertical initial position. If the sample probe is not at
the initial position, reset motor B first.
If the prompt box displays OK, the motor’s normal, if displays FAIL, then the motor’s control
part failure.
 Recorder
Click “Recorder”button, the thermal printer of the instrument will automatically do the printing
test.
 Printer
Click “Printer”button, the external connected printer(If equipped) will automatically do the
printing test.
 Return
The instrument will automatically initialize when returns, as the following diagram：

- 42 -
 Fig. 6-2

6.4.2 Gain Adjustment

Click “Special”in the service menu, input password 6868 in the popped password prompt box
to enter into special interface, as figure 6-3, click the detect window, as figure 6-4:

Fig. 6-3 Special

- 43 -
 Fig. 6-4
 Gain adjustment of WBC channel. Use the 7μm standard particle to test as sample test
method. Observe peak value of WBC histogram around 180fl or not, if not, enter into the
detection interface, click WBC input box to input the adjusted value, then click “H”to save the
modification. Then return to the main measuring interface to test the standard particle again,
observe the peak value of histogram around 180fl or not. Follow this method to adjust the
standard particle peak value to 180fl.
 Gain adjustment of RBC channel. use 5μm standard particle to test, observe WBC value
around 65fl or not. While sampling, use the pipettor to add 20μL standard particle into the
RBC chamber when the probe moves to the lower part of RBC chamber. Then observe the
peak value around 65fL or not. Adjusting method as WBC channel gain adjustment.
 HGB channel adjustment: HGB - VAL is the conversion display mode of HGB voltage, normal
value 3400 (+ 100). Click HGB input box to input the number and click "H" button to save it,
then continuously click "R" button to read RGB-VAL results and adjust HGB - VAL results to
the normal range.
 Motor compensation adjustment
The motors compensation adjustment are the compensation steps in each direction, which
have been adjusted well in the factory. So please do not change it arbitrarily unless there’s big
maintenance on the machine which leads to its change. Then adjust it to the original value as the
screen instructed.

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 Version：A/1

 Screen sensitivity adjustment

TS ABL-VAL、TS COUNT are for screen sensitivity setting, do not change them arbitrarily.

6.5 Instrument Special

6.5.1 Factory Calibration

Under the special interface, choose factory mode:

Fig. 6-5
Return to the main menu, choose the calibration to enter into factory calibration mode, as the
following figures: alternative Auto Calibration or Manual Calibration. Factory calibration coefficient
is calibrated in factory, through calibration, the user calibration coefficient is limited within the
range of requirements.

Fig. 6-6

45
 Fig. 6-7

6.5.2 System Settings

Under the special interface, click system setting logo to enter into the system setting
interface:

Fig. 6-8
Two kinds of species for options, human or animals:

Fig. 6-9

- 46 -
 Click the version information to view or modify the instrument’s version information.

Fig. 6-10
Click“Logo”button to set the instrument’s interface logo:

Fig. 6-11

6.5.3 Continuous Tests

Click continuous tests, then return to the main measure interface. Click F9 button on the
lower right side of the screen to enter into the following interface. Set the loop times or cycle time
to let the instrument do continuous measure:

- 47 -
 Fig. 6-12

6.5.4 All Pulse Graphic

Under the special interface to click “All Pulse Graphic” to view the pulse graphic:

Fig. 6-13

- 48 -
 Fig. 6-14

Fig. 6-15

6.5.5 History Query

Search for record of historical samples, as the following figure: choose the
corresponding record files according to the patients’ testing time, then enter into the review
interface to check the historical records.

- 49 -
 Fig. 6-16

6.5.6 Version Setting

To view or modify the version number of the instrument

Fig. 6-17

6.5.7 Measured Times

To view the instrument’s measured times.

Fig. 6-18

- 50 -
6.5.8 System Adjustment

Adjust parameters coefficient:

Fig. 6-19

6.5.9 Parameters Setting

Set the instrument’s alarm parameters:

Fig. 6-20

- 51 -
6.5.10 Vent Diluent

Click“Vent Diluent”button to enter into diluent-adding amount setting menu, choose the
diluent amount to be added in the prediluent mode, as the following figure: the system defaulted
adding amount is 1000μL.

Fig. 6-21

6.6 Common Faults and Solutions

Faults Reason analysis

Solutions
phenomenon

A 1 Force to unclog；2 Cauterize；3

Aperture A Gem aperture clogged;
Clean the Germ aperture and reinstall.
clogged B Negative pressure chamber leak;
B: Replace the sealing ring.

A Cables of the HGB chamber assembly

disconnected or not; lamp damaged or
A HGB lamp working or not; not;
HGB Fault
B HGB voltage normal or not. B Enter into the mechanical interface,
adjust HGB gain, if it’s too low to adjust
up, replace the lamp.
A Check other items’measure
A If stable, check the HGB voltage, if
stable or not;
HGB unstable, check the aspirating volume
B Enter into the mechanical of
unstable and LYSE adding volume stable or not;
service, click the HGB voltage
measure B If HGB voltage change rate more than
every 2 seconds to check the
0.2V, the lamp should be replaced.
voltage change big or not.

- 52 -
 A Ground wire is well connected or
not;
B Check the shielding box is well
isolated or not(Isolated or not A Ensure it is well connected to the
Background
judgement: pull out the signal cable ground;
High
to check the testing cup’s fixing B Reinstall.
board contacted with the shell or
not, if contacted, it is not well
isolated, otherwise isolated well.)
Ａ To observe sensor indicator light’s
status while with or without the buffle in
Ａ Whether moves forward to only
Negative the middle of negative pressure pump
one direction;
pressure sensor. The light’s on with the buffle and
Ｂ Skids while in movement;
pump skids off without the buffle, otherwise abnormal;
Ｃ Negative pressure piston will
and abnormal B The pinion and rack wear down and
bounce or not at the moment of
sound need to be replaced and installed well;
stopping the measure.
C Obstructed waste pipeline, clear it.

A If no value for HGB, the LYSE

doesn’t prim in proper place; A Replace LYSE pistons’seal ring of the
WBC no B Check there’s value for HGB or sample aspirating chamber, refill the
value or not, if so, check there’s pulse for LYSE until no bubbles in LYSE pipeline;
display * WBC or not. Enter into B If there’s pulse, it could be the Gain’s
special(password 6868) to view the too high, lower it down.
pulse graphic.
Check there’s pulse for RBC or not, If there’s pulse, it could be the Gain’s too
RBC no
enter into special(password 6868) high, lower it down, or the Gain’s too low,
value
to view the pulse graphic. turn it up.
A Ground wire connected well or A Measure the zero ground line voltage
not; of the power cord <3V or not, if not, must
B Whether share the power socket replace the power cord for the ground
with high power devices; lines;
C Ambient temperature meets the B If so, use another socket to separate
PLT
requirements of the instrument or this instrument with other high power
background
not; devices;
too high(One
D Execute regular maintenance or C If the ambient temperature does not
or all of WBC,
not; match the requirements, change the
RBC, PLT
E Whether the reagents expired or ambient temperature to meet the
test results
unclean; requirements;
unstable )
F Check each board, especially Ｄ If the regular maintenance is not
the testing cup assembly rusted or executed, the pipelines could not be
not and testing cup’s fixing plate clean, so flush the pipelines first, then
isolated from the ground well or maintain.
not(Isolated or not judgement): pull Ｅ Replace the reagents;

- 53 -
 out the signal cable to check the Ｆ Derust and reinstall.
testing cup’s fixing plate contacted
with the shell or not, if contacted, it
is not well isolated.

Has pulse but too strong, mostly caused

Whether PLT has the pulse,
by the interference, the method to
PLT no result enter into special (password 6868)
eliminate the interference is the same as
to check pulse graphic.
PLT background.
A Use QC blood to readjust the
A Improper adjusted WBC gain;
Poor histograms of WBC, RBC, PLT;
B Inadequate LYSE adding
differentiation B Check the LYSE’s adding amount
C Abnormal blood samples
C Check the blood samples.
A Unproperly connected display screen
cable plugs and adapters, if not, change
A No display other functions the display;
working normally; B Check the CPU board works normally
White screen
B No display other functions or not(Judgement: power on the CPU
working abnormally. board to work alone, could enter into the
system is OK, otherwise CPU board
damaged)
A Alarm when remove waste liquid
Misreport A Problems with the driver board, replace
sensor
waste liquid the driver board;
B No alarm when remove waste
full B Waste liquid sensor damaged.
liquid sensor
Adjust potentiometer (R104, R107) to get
good voltage for C1 and C2 (test point
T-C1,T-C2) according below requirement:
After some time using, reagent Detector has reagent, voltage range
Diluent, lyse detector container transmission of 0.2V-0.4V; Detector has no reagent,
false alarm light reduced and lead this problem voltage range 0.65V-0.8V. After
adjustment, need to restart analyzer and
test voltage again. If voltage is not in
range, need to adjust again until it is in
range.

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 Add: 6F, Shanshui Building B, Nanshan Yungu Innovation
Industrial Park, 1183 Liuxian Blvd, Nanshan District, Shenzhen, China
Tel : +86 755-26835560
Fax : +86 755-26678789
Web: www.geniusmedica.com
Well Kang Limited
EC REP The Black Church, St. Mary’s Place, Dublin 7, Ireland
www.CE-Marking.EU

Item No.: P01.91.700031-00